Publications by authors named "Yingchun Li"

180 Publications

A photoelectrochemical sensor based on Z-Scheme TiO@Au@CdS and molecularly imprinted polymer for uric acid detection.

Mikrochim Acta 2021 May 15;188(6):188. Epub 2021 May 15.

College of Science, Harbin Institute of Technology (Shenzhen), Shenzhen, Guangdong, 518055, People's Republic of China.

A novel photoelectrochemical (PEC) sensor based on "Z-scheme" TiO@Au@CdS and molecularly imprinted polymer (MIP) was developed for the non-invasive detection of uric acid (UA). The "Z-scheme" material, consisting of an electron-transfer system (Au) and two isolated photochemical systems (CdS, TiO), was synthesized by chemical deposition method and it worked as a substrate for electro-polymerization of MIP. Due to the high photoelectric conversion efficiency provided by TiO@Au@CdS and specific imprinting effect afforded by MIP, the sensor displayed desirable sensing performance with the merits of sensitivity, selectivity, repeatability, and stability. The linear range for UA detection is from 1 nM to 9 μM with the detection limit of 0.3 nM (S/N = 3). Moreover, the assay was successfully utilized to measure UA in human tears and offered a reliable result. The incorporation of MIP and "Z-scheme" material into a PEC sensor system is expected to provide a promising strategy for detecting other small molecules.
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http://dx.doi.org/10.1007/s00604-021-04841-xDOI Listing
May 2021

3'-UTR Polymorphism rs15869 Alters Susceptibility to Papillary Thyroid Carcinoma via Binding hsa-mir-1178-3p.

Pharmgenomics Pers Med 2021 6;14:533-544. Epub 2021 May 6.

Department of Head and Neck Surgery, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, Shenyang, People's Republic of China.

Objective: To investigate the associations of polymorphisms in the following DNA double-strand break repair (DSBR) genes with papillary thyroid carcinoma (PTC) risk (including rs11852786, rs963917, rs12516 and rs8176318, rs15869, rs2035990 and rs2440).

Materials And Methods: A matched case-control study was implemented to examine associations between PTC risk and the above polymorphisms. Subsequently, we evaluated the effects of the potential PTC susceptibility-related variant rs15869 on BRCA2 mRNA secondary structure and BRCA2 expression through bioinformatics analysis and experiment validation. Additionally, luciferase assay was used to identify whether rs15869 polymorphism can substantially affect the binding of hsa-miR-1178-3p to mRNA. Finally, Pearson correlation analysis was performed to determine the correlation between the expression of hsa-miR-1178-3p and BRCA2 mRNA and protein in thyroid tissues harboring rs15869 different genotypes.

Results: rs15869 CC genotype was associated with a higher risk of PTC than its AA genotype. Subsequently, stratified analyses came to the same conclusion in the female or age<50 population. Furthermore, we confirmed that the A-to-C substitution of rs15869 changed BRCA2 mRNA secondary structure and contributed to a decreased BRCA2 expression. Mechanistically, a significantly decreased luciferase activity verified a greater binding between hsa-miR-1178-3p and rs15869 C allele, but not the A allele, which was evidenced by the significant negative correlation between hsa-miR-1178-3p with BRCA2 mRNA and protein levels in thyroid tissues with AC and CC genotype but not AA genotype at rs15869.

Conclusion: rs15869 is characterized as a potential biomarker associated with PTC risk, highlighting the contribution of the hsa-miR-1178-3p via functional exploration.
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http://dx.doi.org/10.2147/PGPM.S300783DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8112253PMC
May 2021

Current Situation and Key Parameters for Improving Wheat Quality in China.

Front Plant Sci 2021 15;12:638525. Epub 2021 Feb 15.

Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing, China.

Processing quality of winter-wheat is affected by genotype, environmental conditions, and crop husbandry practices. In the present study, a data set of 17 quality-related traits for 211 main winter-wheat varieties in China during 2006 to 2018 was extracted from China Wheat Quality Report. Analysis was carried out to evaluate the quality status and variations, to reveal correlation between quality-related traits, as well as to identify key influencing factors. Results indicated that the quality indicators of medium-gluten or medium-strong-gluten wheat varieties were acceptable, whereas those of weak- and strong-gluten wheat varieties were far below national standard, especially hardness index (HI), crude protein content (CPC), wet gluten content (WG), and water absorption for weak-gluten wheat and sedimentation value (SV), stability time (ST), and stretch area (SA) for strong-gluten wheat, respectively. Correlation analysis showed that WA, WG, development time, HI, CPC, falling number, ST, and tractility directly affected the overall quality of winter-wheat. CPC, SV, and WG in medium-gluten wheat had no significant correlation with the processing quality of noodles score, whereas gluten index significantly correlated with noodle score ( < 0.001). This implied that protein quality might play a more important role than protein quantity in determining medium-gluten wheat quality. Furthermore, analysis of variance showed that genetic characteristics (cultivars) had significant influences on the restriction indexes (SV, ST, and SA) of strong-gluten wheat, whereas genetic characteristics, environment conditions, and crop growing practices (cultivars, locations, and years) significantly affected the restriction indexes (HI, CPC, WG, and WA) of weak-gluten wheat. The results suggest that improvement of Chinese strong-gluten wheat should mainly focus on cultivating new varieties. As to weak-gluten wheat, cultivation and husbandry practices should be paid more attention to limit undesired high grain protein content.
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http://dx.doi.org/10.3389/fpls.2021.638525DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7917211PMC
February 2021

Spatial distribution and determinants of health loss from Kashin-Beck disease in Bin County, Shaanxi Province, China.

BMC Public Health 2021 Feb 19;21(1):387. Epub 2021 Feb 19.

Key Laboratory of Land Surface Pattern and Simulation, Institute of Geographical Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing, 100101, China.

Background: Kashin-Beck disease (KBD) is one of the major endemic diseases in China, which severely impacts the physical health and life quality of people. A better understanding of the spatial distribution of the health loss from KBD and its influencing factors will help to identify areas and populations at high risk so as to plan for targeted interventions.

Methods: The data of patients with KBD at village-level were collected to estimate and analyze the spatial pattern of health loss from KBD in Bin County, Shaanxi Province. The years lived with disability (YLDs) index was applied as a measure of health loss from KBD. Spatial autocorrelation methodologies, including Global Moran's I and Local Moran's I, were used to describe and map spatial clusters of the health loss. In addition, basic individual information and environmental samples were collected to explore natural and social determinants of the health loss from KBD.

Results: The estimation of YLDs showed that patients with KBD of grade II and patients over 50 years old contributed most to the health loss of KBD in Bin County. No significant difference was observed between two genders. The spatial patterns of YLDs and YLD rate of KBD were clustered significantly at both global and local scales. Villages in the southwestern and eastern regions revealed higher health loss, while those in the northern regions exhibited lower health loss. This clustering was found to be significantly related to organically bound Se in soil and poverty rate of KBD patients.

Conclusions: Our results suggest that future treatment and prevention of KBD should focus on endemic areas with high organically bound Se in soil and poor economic conditions. The findings can also provide important information for further exploration of the etiology of KBD.
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http://dx.doi.org/10.1186/s12889-021-10407-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7893884PMC
February 2021

Intra-Individual Comparison of 18F-PSMA-1007 and 18F-FDG PET/CT in the Evaluation of Patients With Prostate Cancer.

Front Oncol 2020 1;10:585213. Epub 2021 Feb 1.

Radiation Oncology Key Laboratory of Sichuan Province, PET/CT Centre, Sichuan Cancer Hospital, Chengdu, China.

Purpose: 18F labelled PSMA-1007 presents promising results in detecting prostate cancer (PC), while some pitfalls exists meanwhile. An intra-individual comparison of 18F-FDG and 18F-PSMA-1007 in patients with prostate cancer were aimed to be performed in the present study. Then, the pitfalls of 18F-PSMA-1007 PET/CT in imaging of patients with prostate cancer were analyzed.

Methods And Material: 21 prostate cancer patients underwent 18F-PSMA-1007 PET/CT as well as 18F-FDG PET/CT before treatment. All positive lesions were noticed in both 18F-PSMA-1007 PET/CT and 18F-FDG PET/CT, then differentiated PC metastasis from benign lesions. the SUVmax, SUVmean and TBR of lesions, up to 10 metastases and 10 benign lesions per patients were recorded (5 for bone, 5 for soft tissue metastasis ). The distribution of positive lesions were analyzed for two imaging. Detection rates, SUVmax, SUVmean and TBR in 18F-PSMA-1007 PET/CT and 18F-FDG PET/CT were compared, respectively. The optimal cut-off values of SUVmax, SUVmean for metastases vs. benign lesions was found through areas under ROC in 18F-PSMA-1007.

Results: The detection rates of primary lesions in 18F-PSMA-1007 PET/CT was higher than that of 18F-FDG PET/CT(100% (21/21) vs. 67%(14/21)). For extra- prostatic lesions, 18F-PSMA-1007 PET/CT revealed 124 positive lesions, 49(49/124, 40%) attributed to a benign origin; 18F-FDG PET/CT revealed 68 positive lesions, 14(14/68, 21%) attributed to a benign origin. The SUVmax, SUVmean, TBR of primary tumor in 18F-PSMA-1007 PET/CT was higher than that in 18F-FDG PET/CT (15.20 vs. 4.20 for SUVmax; 8.70 vs. 2.80 for SUVmean; 24.92 vs. 4.82 for TBR, respectively); The SUVmax, SUVmean, TBR of metastases in 18F-PSMA-1007 PET/CT was higher than that in 18F-FDG PET/CT (10.72 vs. 4.42 for SUVmax; 6.67 vs. 2.59 for SUVmean; The TBR of metastases was 13.3 vs. 7.91). For 18F-FDG PET/CT, the SUVmax, SUVmean in metastases was higher than that in benign lesions (4.42 vs. 3.04 for SUVmax, 2.59 vs. 1.75 for SUVmean, respectively). Similarly, for 18F-PSMA-1007 PET/CT, the SUVmax, SUVmean in metastases was significantly higher than that in benign lesions(10.72 vs. 3.14 for SUVmax, 6.67 vs. 1.91 for SUVmean, respectively), ROC suggested that SUVmax=7.71, SUVmean=5.35 might be the optimal cut-off values for metastases vs. benign lesions.

Conclusion: The pilot study suggested that 18F-PSMA-1007 showed superiority over 18F-FDG because its high detecting rate of PC lesions and excellent tumor uptake. While non-tumor uptake in 18F-PSMA-1007 may lead to misdiagnosis, recognizing these pitfalls and careful analysis can improve the accuracy of diagnosis.
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http://dx.doi.org/10.3389/fonc.2020.585213DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7884904PMC
February 2021

Transient receptor potential vanilloid 4 activation inhibits the delayed rectifier potassium channels in hippocampal pyramidal neurons: An implication in pathological changes following pilocarpine-induced status epilepticus.

J Neurosci Res 2021 Mar 4;99(3):914-926. Epub 2020 Dec 4.

Department of Physiology, Nanjing Medical University, Nanjing, P.R. China.

Activation of transient receptor potential vanilloid 4 (TRPV4) can increase hippocampal neuronal excitability. TRPV4 has been reported to be involved in the pathogenesis of epilepsy. Voltage-gated potassium channels (VGPCs) play an important role in regulating neuronal excitability and abnormal VGPCs expression or function is related to epilepsy. Here, we examined the effect of TRPV4 activation on the delayed rectifier potassium current (I ) in hippocampal pyramidal neurons and on the Kv subunits expression in male mice. We also explored the role of TRPV4 in changes in Kv subunits expression in male mice following pilocarpine-induced status epilepticus (PISE). Application of TRPV4 agonists, GSK1016790A and 5,6-EET, markedly reduced I in hippocampal pyramidal neurons and shifted the voltage-dependent inactivation curve to the hyperpolarizing direction. GSK1016790A- and 5,6-EET-induced inhibition of I was blocked by TRPV4 specific antagonists, HC-067047 and RN1734. GSK1016790A-induced inhibition of I was markedly attenuated by calcium/calmodulin-dependent kinase II (CaMKII) antagonist. Application of GSK1016790A for up to 1 hr did not change the hippocampal protein levels of Kv1.1, Kv1.2, or Kv2.1. Intracerebroventricular injection of GSK1016790A for 3 d reduced the hippocampal protein levels of Kv1.2 and Kv2.1, leaving that of Kv1.1 unchanged. Kv1.2 and Kv2.1 protein levels as well as I reduced markedly in hippocampi on day 3 post PISE, which was significantly reversed by HC-067047. We conclude that activation of TRPV4 inhibits I in hippocampal pyramidal neurons, possibly by activating CaMKII. TRPV4-induced decrease in Kv1.2 and Kv2.1 expression and I may be involved in the pathological changes following PISE.
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http://dx.doi.org/10.1002/jnr.24749DOI Listing
March 2021

Generation of Thyroid Tissues From Embryonic Stem Cells Blastocyst Complementation .

Front Endocrinol (Lausanne) 2020 14;11:609697. Epub 2020 Dec 14.

Department of Medical Oncology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.

The generation of mature, functional, thyroid follicular cells from pluripotent stem cells would potentially provide a therapeutic benefit for patients with hypothyroidism, but differentiation remains difficult. We earlier reported the generation of lung organs blastocyst complementation in fibroblast growth factor 10 (), compound, heterozygous mutant ( Ex1/Ex3) mice. Fgf10 also plays an essential role in thyroid development and branching morphogenesis, but any role thereof in thyroid organogenesis remains unclear. Here, we report that the thyroids of Ex1/Ex3 mice exhibit severe hypoplasia, and we generate thyroid tissues from mouse embryonic stem cells (ESCs) in Ex1/Ex3 mice blastocyst complementation. The tissues were morphologically normal and physiologically functional. The thyroid follicular cells of Ex1/Ex3 chimeric mice were derived largely from GFP-positive mouse ESCs although the recipient cells were mixed. Thyroid generation blastocyst complementation will aid functional thyroid regeneration.
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http://dx.doi.org/10.3389/fendo.2020.609697DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767966PMC
December 2020

Selenium Nutritional Status of Rural Residents and Its Correlation with Dietary Intake Patterns in a Typical Low-Selenium Area in China.

Nutrients 2020 Dec 13;12(12). Epub 2020 Dec 13.

Centre for Disease Prevention and Control of Binxian County, Xianyang 713500, China.

China is recognized as a selenium-deficient country, and nutritional selenium intake has always been a concern. To clarify the current inhabitants' selenium nutrition status and the characteristics of dietary consumption in low-selenium areas, samples of human hair and grains were collected, and food frequency questionnaires were administered in Binxian County, Shaanxi Province, a typical low-selenium area in the Loess Plateau. The subject number of the study is 85, and the age range is from 11 to 81 years, with an average of 60. The results showed that the average hair selenium content of the residents was 231.7 μg/kg, and 62.4% of the participants had levels higher than the selenium deficiency threshold (200 μg/kg). There was a significant positive correlation between the hair selenium content and the food consumption score after adjusting for rice outsourcing. Three different dietary patterns were noted according to hierarchical cluster analysis. This study provides a tool for assessing the selenium nutrition of inhabitants in low-selenium areas and has considerable significance for improving the dietary pattern of residents.
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http://dx.doi.org/10.3390/nu12123816DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764644PMC
December 2020

Novel long noncoding RNA LINC02323 promotes cell growth and migration of ovarian cancer via TGF-β receptor 1 by miR-1343-3p.

J Clin Lab Anal 2021 Feb 28;35(2):e23651. Epub 2020 Nov 28.

Department of Gynaecology, CangZhou Central Hospital, Cangzhou City, China.

Background: This study was aimed at investigating the effects of long noncoding RNA (lncRNA) LINC02323 in ovarian cancer and its possible mechanism.

Methods: Microarray analysis and QPCR were utilized to identify lncRNA LINC02323 expression in patients with ovarian cancer. MTT assay was used for analysis of ovarian cancer cell proliferation. Western blot was utilized to investigate its possible mechanism.

Results: In patients with ovarian cancer, lncRNA LINC02323 expression was up-regulated and miR-1343-3p expression was down-regulated. Over-expression of lncRNA LINC02323 promoted cell growth and reduced LDH activity levels in vitro model by suppression of miR-1343-3p expression. Down-regulation of lncRNA LINC02323 reduced cell growth and increased LDH activity levels in vitro model by induction of miR-1343-3p expression. Over-expression of miR-1343-3p reduced cell growth and reduced LDH activity levels in vitro model by suppression of TGF-β receptor. Down-regulation of miR-1343-3p promoted cell growth and reduced LDH activity levels in vitro model by induced of TGF-β receptor.

Conclusion: Our findings show that Novel long noncoding RNA LINC02323 promotes cell growth of ovarian cancer via TGF-β receptor 1 by miR-1343-3p.
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http://dx.doi.org/10.1002/jcla.23651DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7891524PMC
February 2021

Differentiated mineral nutrient management in two bamboo species under elevated CO environment.

J Environ Manage 2021 Feb 5;279:111600. Epub 2020 Nov 5.

Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, 311400, Zhejiang, China. Electronic address:

Mineral nutrients play a critical role in maintaining plant growth, but are vulnerable to climate change, such as elevated atmospheric carbon dioxide (CO) concentrations. Previous studies reported that impact of elevated CO concentrations on plant growth vary among plant species, which may affect differential mineral nutrient cycling among plant species. However, little is known about how increasing CO concentrations affect mineral nutrient uptake and allocation in bamboo species. Using open top chambers (OTCs), we investigated the effects of elevated CO concentrations on three key mineral nutrients (iron (Fe), calcium (Ca), and magnesium (Mg)) in two mature bamboo species (Phyllostachys edulis and Oligostachyum lubricum). Results showed increased leaf and root biomass under elevated CO concentrations (P. edulis: 30.24% and 10.94%; O. lubricum: 24.47% and 13.84%, respectively). Conversely, elevated CO concentrations had negligible effects on the biomass of other bamboo organs (e.g., branches and culms). To a certain extent, elevated CO concentrations also caused nutrient variation among the various organs of these two species. For Ph. edulis, elevated CO concentrations increased mineral content (Fe, Ca, and Mg) in and allocation to leaves while it decreased Fe and Mg allocation to roots. By contrast, elevated CO concentrations only increased mineral content in and allocation to O. lubricum leaves and decreased Mg to its roots. Results confirmed that elevated CO concentrations resulted in differential mineral nutrient uptake and allocation response between these two species. Understanding such differences is critical to the sustainable nutrient management of bamboo ecosystems under increasing CO concentrations.
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http://dx.doi.org/10.1016/j.jenvman.2020.111600DOI Listing
February 2021

Quick Automatic Synthesis of Solvent-Free 16α-[F] Fluoroestradiol: Comparison of Kryptofix 222 and Tetrabutylammonium Bicarbonate.

Front Oncol 2020 25;10:577979. Epub 2020 Sep 25.

PET/CT Center, Sichuan Cancer Center, School of Medicine, Sichuan Cancer Hospital & Institute, University of Electronic Science and Technology of China, Chengdu, China.

Estrogen receptor (ER) expression level of human breast cancer often reflects the stage of disease and is usually monitored by immunohistochemical staining . The preferable non-invasive and real-time diagnosis is more accessible by PET scan using 16α-[F]FES. The objective of this study was to develop a quick automatic method for synthesis of solvent-free 16α-[F]FES using a CFN-MPS-200 synthesis system and compare the catalytic efficiency of two phase transfer catalysts, Kryptofix 222/KCO (K222/KCO) and tetrabutylammonium hydrogen carbonate (TBA·HCO). In this method, phase transfer catalysts K222/KCO and TBA·HCO were used, respectively. The intermediate products were both hydrolyzed with hydrochloric acid and neutralized with sodium bicarbonate. The crude product was purified with semi-preparative HPLC, and the solvent was removed by rotary evaporation. The effects of radiofluorination temperature and time on the synthesis were also investigated. Radiochemical purity of solvent-free product was above 99% and the decay-corrected radiochemical yield of 16α-[F]FES was obtained in 48.7 ± 0.95% (catalyzed by K222/KCO, = 4) and 46.7 ± 0.77% (catalyzed by TBA·HCO, = 4, respectively). The solvent-free 16α-[F]FES was studied in clinically diagnosed breast cancer patients, and FES-PET results were compared with pathology diagnosis results to validate the diagnosis value of 16α-[F]FES. The new method was more reliable, efficient, and time-saving. There was no significant difference in catalytic activity between K222/KCO and TBA·HCO.
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http://dx.doi.org/10.3389/fonc.2020.577979DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7546761PMC
September 2020

The Regulation of circRNA RNF13/miRNA-1224-5p Axis Promotes the Malignant Evolution in Acute Myeloid Leukemia.

Biomed Res Int 2020 6;2020:5654380. Epub 2020 Oct 6.

Department of Hematology, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China.

Objective: To study the biological function of circular RNA RNF13 (circRNF13) in acute myeloid leukemia (AML) and its relationship with prognosis.

Methods: We constructed stable AML cell lines with downregulated expression of circRNF13, and then, we explored the effect of downregulation of circRNF13 expression on the proliferation, migration, and invasion through qRT-PCR, MTT curve, colony formation, transwell migration and invasion experiment, cell cycle, apoptosis, Caspase 3/7 assay, and other experiments. We also studied the expression of C-myc and Tenascin-C by qRT-PCR to explore the role of circRNF13.

Results: When the expression of circRNF13 was downregulated, the proliferation rate of AML cells decreased significantly, the cell cycle was blocked to G1 phase, and apoptosis rate increased significantly. C-myc related to cell proliferation decreased significantly at RNA level. Furthermore, when the expression of circRNF13 was downregulated, the migration and invasion ability of AML cells was significantly reduced, and the expression of Tenascin-C related to migration and invasion also decreased significantly. The luciferase reporter assay system confirmed that miRNA-1224-5p was the direct target of circRNF13.

Conclusion: CircRNF13 inhibited the proliferation, migration, and invasion of AML cells by regulating the expression of miRNA-1224-5p. This study provides some clues for the diagnosis and treatment of AML.
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http://dx.doi.org/10.1155/2020/5654380DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7557902PMC
May 2021

Type 2 and interferon inflammation regulate SARS-CoV-2 entry factor expression in the airway epithelium.

Nat Commun 2020 10 12;11(1):5139. Epub 2020 Oct 12.

Center for Genes, Environment, and Health, National Jewish Health, Denver, CO, USA.

Coronavirus disease 2019 (COVID-19) is caused by SARS-CoV-2, an emerging virus that utilizes host proteins ACE2 and TMPRSS2 as entry factors. Understanding the factors affecting the pattern and levels of expression of these genes is important for deeper understanding of SARS-CoV-2 tropism and pathogenesis. Here we explore the role of genetics and co-expression networks in regulating these genes in the airway, through the analysis of nasal airway transcriptome data from 695 children. We identify expression quantitative trait loci for both ACE2 and TMPRSS2, that vary in frequency across world populations. We find TMPRSS2 is part of a mucus secretory network, highly upregulated by type 2 (T2) inflammation through the action of interleukin-13, and that the interferon response to respiratory viruses highly upregulates ACE2 expression. IL-13 and virus infection mediated effects on ACE2 expression were also observed at the protein level in the airway epithelium. Finally, we define airway responses to common coronavirus infections in children, finding that these infections generate host responses similar to other viral species, including upregulation of IL6 and ACE2. Our results reveal possible mechanisms influencing SARS-CoV-2 infectivity and COVID-19 clinical outcomes.
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http://dx.doi.org/10.1038/s41467-020-18781-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7550582PMC
October 2020

Prenatal Stress Leads to the Altered Maturation of Corticostriatal Synaptic Plasticity and Related Behavioral Impairments Through Epigenetic Modifications of Dopamine D2 Receptor in Mice.

Mol Neurobiol 2021 Jan 15;58(1):317-328. Epub 2020 Sep 15.

Department of Physiology, Nanjing Medical University, Longmian Avenue 101, Jiangning District, Nanjing City, 211166, Jiangsu Province, China.

Prenatal stress (PRS) had a long-term adverse effect on motor behaviors. Corticostriatal synaptic plasticity, a cellular basis for motor controlling, has been proven to participate in the pathogenesis of many behavior disorders. Based on the reports about the involvement of epigenetic DNA alterations in PRS-induced long-term effects, this research investigated the influence of PRS on the development and maturation of corticostriatal synaptic plasticity and related behaviors and explored the underlying epigenetic mechanism. Subjects were male offspring of dams that were exposed to stress three times per day from the 10th day of pregnancy until delivery. The development and maturation of plasticity at corticostriatal synapses, dopamine signaling, behavioral habituation, and DNA methylation were examined and analyzed. Control mice expressed long-term potentiation (LTP) at corticostriatal synapses during postnatal days (PD) 12-14 and produced long-term depression (LTD) during PD 20-60. However, PRS mice exhibited sustained LTP during PD 12-60. The treatment with dopamine 2 receptor (D2R) agonist quinpirole recovered striatal LTD and improved the impaired behavioral habituation in PD 45 adult PRS mice. Additionally, adult PRS mice showed reduced D2R, excess DNA methyltransferase 1 (DNMT1), increased binding of DNMT1 to D2R promoter, and hypermethylation at D2R promoter in the striatum. The DNMT1 inhibitor 5-aza-deoxycytidine restored striatal synaptic plasticity and improved behavioral habituation in adult PRS mice via D2R-mediated dopamine signaling. DNMT1-associated D2R hypermethylation is responsible for altering the maturation of plasticity at corticostriatal synapses and impairing the behavioral habituation in PRS mice.
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http://dx.doi.org/10.1007/s12035-020-02127-6DOI Listing
January 2021

A ratiometric electrochemiluminescence sensing platform for robust ascorbic acid analysis based on a molecularly imprinted polymer modified bipolar electrode.

Biosens Bioelectron 2020 Nov 31;167:112490. Epub 2020 Jul 31.

College of Science, Harbin Institute of Technology, Shenzhen, Guangdong, 518055, PR China; College of Optoelectronic Engineering, Shenzhen University, Shenzhen, Guangdong, 518060, PR China. Electronic address:

Herein, a novel molecularly imprinted polymer (MIP) modified spatial-resolved "on-off" ratiometric electrochemiluminescence (ECL) sensing platform based on a closed bipolar electrode (BPE) has been reported for highly accurate and selective detection of ascorbic acid (AA). AA-imprinted MIP was decorated on the anode of the BPE, and Ru (bpy) in the anode electrolyte served as anode-emitter, while ZnInS as the other ECL emitter was coated on the cathode. Rebinding of AA at anode promoted ECL response of ZnInS (440 nm) at cathode. Meanwhile, the ECL response at 605 nm decreased, arising from the hindered reaction of Ru (bpy) on the anode surface. Therefore, an "on-off" BPE-ECL sensing platform was fabricated and showed distinguished performance in repeatability and selectivity thanks to the ratio correction effect and the specific recognition from MIP. The linear range for AA detection is from 50 nM to 3 μM with a low detection limit of 20 nM (S/N = 3). The assay deviation of the ratio responses largely declined by about 15 and 5 times compared with the ones from single pole in the aspect of repeatability and long-term stability, respectively. This work provides a reliable and stable sensing pattern for practical application, which also furnishes a strategy for designing simple and low-cost ECL sensing devices.
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http://dx.doi.org/10.1016/j.bios.2020.112490DOI Listing
November 2020

Electrochemical sensor based on dual-template molecularly imprinted polymer and nanoporous gold leaf modified electrode for simultaneous determination of dopamine and uric acid.

Mikrochim Acta 2020 08 15;187(9):496. Epub 2020 Aug 15.

College of Science, Harbin Institute of Technology (Shenzhen), Shenzhen, 518055, Guangdong, People's Republic of China.

A novel electrochemical sensor based on dual-template molecularly imprinted polymer (MIP) with nanoporous gold leaf (NPGL) was established for the simultaneous determination of dopamine (DA) and uric acid (UA). NPGL acts as an enlarged loading platform to enhance sensing capacity, and the MIP layer was synthesized in situ in the presence of monomer and dual templates (DA and UA) to provide specific recognition. Under the optimal conditions, the sensor shows a good linear range of 2.0~180 μM for DA at a working potential of 0.15 V (vs. Ag/AgCl) and 5.0~160 μM for UA at 0.35 V (vs. Ag/AgCl), with the respective detection limit of 0.3 μM and 0.4 μM (S/N = 3). Good selectivity of the sensor to its dual templates was confirmed as the sensing signals are significantly different between templates and interfering species. The responses maintained higher than 96% of the initial values after 30-day storage, and the day-to-day relative standard deviation is less than 3.0%. Real sample simultaneous determination of DA and UA was conducted with bovine serum, and the results were in good agreement with those from high-performance liquid chromatography. It can be concluded that this work offers a reliable, facile, fast, and cost-effective method of simultaneous quantification of two or more chem-/bio-molecules. Graphical abstract.
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http://dx.doi.org/10.1007/s00604-020-04413-5DOI Listing
August 2020

Application of Multiplex Microfluidic Electrochemical Sensors in Monitoring Hematological Tumor Biomarkers.

Anal Chem 2020 09 19;92(17):11981-11986. Epub 2020 Aug 19.

School of Science, Harbin Institute of Technology, Shenzhen 518055, China.

Rapid yet accurate detection of disease-related biomarkers is key for point of care testing, where there is an increasing demand for multi-index analysis. Here, we present a versatile device for multianalyte quantification based on the microfluidic technique and electrochemical sensor array. The analytes were shunted through inner-built channels to screen-printed electrodes installed at different positions of the chip. These electrodes were modified with different nanomaterials and sensing agents to afford specific responses to the corresponding indicators. To prove the applicability of the platform for multifunction, we chose leukemia as the model disease and determined four relevant markers: methotrexate (MTX), lactate dehydrogenase (LDH), uric acid (UA), and urea. They are indicative as/for the therapeutic drug (MTX), prognosis (LDH), and side effect (UA and urea). The sensing chip exhibited low detection limits of 35 nM, 25 U/L, 450 nM, and 20 μM toward the four analytes, which are much lower than their minimum contents in human serum. Furthermore, practical application of the chip was demonstrated by simultaneous detection of the four analytes in the blood plasma of rabbit. By simply replacing the modification agents, the sensing platform is expected to serve the detection of a wide range of chem/biosubstances in various fields.
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http://dx.doi.org/10.1021/acs.analchem.0c02430DOI Listing
September 2020

Heavy Metals in Grains from Jilin Province, China, and Human Health Risk.

J Food Prot 2020 Dec;83(12):2193-2199

Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, School of Life Science, Jilin University, Changchun, 130021, People's Republic of China.

Abstract: Heavy metals are an indispensable part of industrial and agricultural development. As the cradle of China's industry and an important province for agricultural production, Jilin Province has been an area of concern about heavy metal pollution in the local environment and grains. In this study, we focused on four heavy metals that are harmful to humans: arsenic (As), cadmium (Cd), methylmercury (MeHg), and inorganic arsenic (iAs). We determined the contents of these metals in 341 grain samples by using graphite furnace atomic absorption spectrometry and liquid chromatography-atomic fluorescence spectrometry and compared our results with the limit value of national standards. To evaluate the potential risk to human health, we determined the target hazard quotient and hazard index. Heavy metals were detected at these rates, from high to low: Cd (48%) > iAs (20.8%) > MeHg (4.6%) > Pb (3%). Most of these values are far below the limit of national standards. The target hazard quotient and hazard index were both smaller than 1; thus, we conclude that heavy metal pollution in grains in Jilin Province is not serious and that people are not at high risk from heavy metals in grains.

Highlights:
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http://dx.doi.org/10.4315/JFP-20-075DOI Listing
December 2020

Risk Stratification Based on Chronic Liver Failure Consortium Acute Decompensation Score in Patients With Child-Pugh B Cirrhosis and Acute Variceal Bleeding.

Hepatology 2021 Apr 23;73(4):1478-1493. Epub 2021 Jan 23.

State Key Laboratory of Cancer Biology, National Clinical Research Center for Digestive Diseases and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an, China.

Background And Aims: Optimal candidates for early transjugular intrahepatic portosystemic shunt (TIPS) in patients with Child-Pugh B cirrhosis and acute variceal bleeding (AVB) remain unclear. This study aimed to test the hypothesis that risk stratification using the Chronic Liver Failure Consortium Acute Decompensation score (CLIF-C ADs) may be useful to identify a subgroup at high risk of mortality or further bleeding that may benefit from early TIPS in patients with Child-Pugh B cirrhosis and AVB.

Approach And Results: We analyzed the pooled individual data from two previous studies of 608 patients with Child-Pugh B cirrhosis and AVB who received standard treatment between 2010 and 2017 in China. The concordance index values of CLIF-C ADs for 6-week and 1-year mortality (0.715 and 0.708) were significantly better than those of active bleeding at endoscopy (0.633 [P < 0.001] and 0.556 [P < 0.001]) and other prognostic models. With X-tile software identifying an optimal cutoff value, patients were categorized as low risk (CLIF-C ADs <48), intermediate risk (CLIF-C ADs 48-56), and high risk (CLIF-C ADs >56), with a 5.6%, 16.8%, and 25.4% risk of 6-week death, respectively. Nevertheless, the performance of CLIF-C ADs for predicting a composite endpoint of 6-week death or further bleeding was not satisfactory (area under the receiver operating characteristics curve [AUC], 0.588). A nomogram incorporating components of CLIF-C ADs and albumin, platelet, active bleeding, and ascites significantly improved the prediction accuracy (AUC, 0.725).

Conclusions: In patients with Child-Pugh B cirrhosis and AVB, risk stratification using CLIF-C ADs identifies a subgroup with high risk of death that may derive survival benefit from early TIPS. With improved prediction accuracy for 6-week death or further bleeding, the data-driven nomogram may help to stratify patients in randomized trials. Future external validation of these findings in patients with different etiologies is required.
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http://dx.doi.org/10.1002/hep.31478DOI Listing
April 2021

Recent advances of two-dimensional materials in smart drug delivery nano-systems.

Bioact Mater 2020 Dec 15;5(4):1071-1086. Epub 2020 Jul 15.

Shenzhen Engineering Laboratory of Phosphorene and Optoelectronics, Collaborative Innovation Center for Optoelectronic Science &Technology, International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education, Shenzhen University, Shenzhen, 518060, China.

Smart drug delivery nano-systems show significant changes in their physical or chemical properties in response to slight change in environmental physical and/or chemical signals, and further releasing drugs adjusted to the progression of the disease at the right target and rate intelligently. Two-dimensional materials possess dramatic status extend all over various scientific and technological disciplines by reason of their exceptional unique properties in application of smart drug delivery nano-systems. In this review, we summarized current progress to highlight various kinds of two-dimensional materials drug carriers which are widely explored in smart drug delivery systems as well as classification of stimuli responsive two-dimensional materials and the advantages and disadvantages of their applications. Consequently, we anticipate that this review might inspire the development of new two-dimensional materials with smart drug delivery systems, and deepen researchers' understanding of smart nano-carries based on two-dimensional materials.
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http://dx.doi.org/10.1016/j.bioactmat.2020.06.012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7363990PMC
December 2020

Optoelectronic Gas Sensor Based on Few-Layered InSe Nanosheets for NO Detection with Ultrahigh Antihumidity Ability.

Anal Chem 2020 08 4;92(16):11277-11287. Epub 2020 Aug 4.

School of Science, Harbin Institute of Technology, Shenzhen 518055, China.

Two-dimensional (2D) transition-metal/metal chalcogenides including MoS, MoSe, WS, SnS, etc. have shown considerable potential for the fabrication of gas sensors for NO detection. However, these sensors usually suffer from sluggish and incomplete recovery at room temperature, and their sensitivities are limited by presorbed O. In this work, a novel optoelectronic gas sensor based on direct-bandgap InSe nanosheets was demonstrated. Because of the excellent photoelectric and sensing properties in few-layer InSe, detection of NO at room temperature was realized. Ultrahigh and reversible responses were obtained under ultraviolet (UV) light illumination, and the limit of detection (0.98 ppb) was ∼40 times lower than that observed without UV light. Furthermore, the effects of O and HO molecules on sensor performance were fully studied through experiments and density functional theory. Some new mechanisms of NO detection in high relative humidity conditions under UV illumination were proposed, including regulation of proton transfer and induction of HO reduction. In all, this work not only broadens the application field of 2D InSe, but also demonstrates the potential prospect of detecting ppb-level NO in complex circumstances such as human breath by using 2D material-based sensors with light activation.
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http://dx.doi.org/10.1021/acs.analchem.0c01941DOI Listing
August 2020

An electrochemical sensor based on MOF-derived NiO@ZnO hollow microspheres for isoniazid determination.

Mikrochim Acta 2020 06 9;187(7):380. Epub 2020 Jun 9.

Key Laboratory of Xinjiang Phytomedicine Resources for Ministry of Education, School of Pharmacy, Shihezi University, Shihezi, 832000, China.

The electrocatalytic activity of metal-organic framework (MOF)-derived NiO@ZnO hollow microspheres was studied for its application to an isoniazid sensor. The MOF-derived NiO@ZnO hollow spheres were synthesized by the coordination reaction of terephthalic acid with Zn and Ni and followed by calcination. Morphology characterization showed that the MOF-derived NiO@ZnO sphere has circular core-shell structure with pores on its surface. Further electrochemical characterization of the prepared sensor by cyclic voltammetry and differential pulse voltammetry proved that the material has good electrical conductivity and strong catalytic ability. Distinct oxidation peaks occur for INZ at potential of 0.22 V (vs. saturated calomel electrode). Under the optimal experimental conditions, the linear range of the sensor for isoniazid determination was 0.8 ~ 800 μM, and the detection limit was 0.25 μM (S/N = 3). In addition, the sensor displayed good stability, repeatability, and reproducibility. The established method was successfully applied for determination of isoniazid in tablets and mouse serum with admirable accuracy and reliability. Graphical abstract Schematic presentation of an electrochemical sensor based on MOF-derived NiO@ZnO hollow microspheres for isoniazid determination.
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http://dx.doi.org/10.1007/s00604-020-04305-8DOI Listing
June 2020

High fidelity underwater wireless optical communication with a phase-conjugated frame structure.

Appl Opt 2020 May;59(13):4000-4007

In this paper, the influence factors of phase noise are first analyzed in detail and verified by experiments, and its principle is theoretically derived. Besides, we propose a novel, to the best of our knowledge, frame structure for m-quadrature amplitude modulation-orthogonal frequency division multiplexing (m-QAM-OFDM) in underwater wireless optical communication. The frame structure contains OFDM signals and their phase-conjugated signals. At the receiving end, by the simple superposition of phase-conjugated symbols, the noise suppression can be achieved. The feasibility is experimentally demonstrated by transmitting m-QAM-OFDM signal in different modulation formats and scenarios. The results show that bit error rate performance can be significantly improved, and there is also a significant increase in transmission capacity compared to the traditional phase-conjugated method. Moreover, the proposed frame structure can provide a robust and simple compromise between transmission capacity and distance.
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http://dx.doi.org/10.1364/AO.388536DOI Listing
May 2020

Smart Glove Integrated with Tunable MWNTs/PDMS Fibers Made of a One-Step Extrusion Method for Finger Dexterity, Gesture, and Temperature Recognition.

ACS Appl Mater Interfaces 2020 May 14;12(21):23764-23773. Epub 2020 May 14.

Flexible wearable devices have proven to be emerging tools for motion monitoring, personal healthcare, and rehabilitation training. The development of a multifunctional, flexible sensor and the integration of sensors and a smart chip for signal reading and transmission play a critical role in building a smart wearable device. In this work, a smart glove based on multiwalled carbon nanotubes/poly(dimethylsiloxane) (MWNTs/PDMS) fibers is developed for gesture and temperature recognition. First, the well-tunable, stretchable, and thermal-sensitive MWNTs/PDMS fibers are fabricated via a facile and cost-effective one-step extrusion method. The obtained fibers exhibit an outstanding linear relationship between resistance change and strain in the range of 0-120% and excellent cyclic stability and durability after 20 000 cycles of 50% tension. They also present a linear relationship of resistance change and temperature of 0.55% °C with a correlation coefficient of 0.998 in the range of 0-100 °C. The fibers, as parts of wearable sensors, are then integrated into a smart glove along with a custom-made data acquisition chip to recognize finger dexterity, gestures, and temperature signals and output them through a screen display, an audio system, and Bluetooth transmission. The highly integrated, low-cost, and multifunctional glove holds great potential for various applications, such as sign language recognition, rehabilitation training, and telemedicine in the Internet-of-Things era.
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http://dx.doi.org/10.1021/acsami.0c08114DOI Listing
May 2020

Epigenetic Modifications of GABAergic Interneurons Contribute to Deficits in Adult Hippocampus Neurogenesis and Depression-Like Behavior in Prenatally Stressed Mice.

Int J Neuropsychopharmacol 2020 04;23(4):274-285

Department of Physiology, Nanjing Medical University, Jiangsu, China.

Background: Prenatal stress (PRS) is considered a risk factor for depressive disorder. Adult hippocampal neurogenesis is believed to play a role in the regulation of affective behaviors. GABAergic interneuron is a key modulator in adult hippocampal neurogenesis. Growing evidence indicates that PRS has adverse effects on adult hippocampal neurogenesis and DNA epigenetic modifications of the GABAergic system. The aim of this study was to investigate whether epigenetic GABAergic dysfunction participates in the negative impact of PRS on adult hippocampal neurogenesis and related emotional behaviors.

Methods: Behavioral tests were used to explore PRS-induced depression-like behaviors of adult female mice. Immunohistochemistry staining, real-time reverse transcription-polymerase chain reaction, western blot, and chromatin immunoprecipitation were employed to detect adult neurogenesis and epigenetic changes of the GABAergic system in the hippocampus of PRS mice.

Results: PRS mice developed a depression phenotype accompanied by the inhibited maturation of hippocampal newborn neurons. Compared with control mice, PRS mice showed decreased expression of glutamic acid decarboxylase 67 at the mRNA and protein levels. GABAA receptor agonist phenobarbital could rectify the decrease of 5-bromo-2-deoxyuridine/neuronal nuclei double-positive (BrdU+/NeuN+) cells in PRS mice. PRS mice also showed increased expression of DNA methyltransferase 1 and increased binding of DNA methyltransferase 1 to glutamic acid decarboxylase 67 promoter region. The treatment with DNA methyltransferase 1 inhibitor 5-aza-deoxycytidine restored the decrease of BrdU+/NeuN+ cells and depression-like behaviors in PRS mice via improving GABAergic system.

Conclusions: The present results indicate that epigenetic changes of the GABAergic system are responsible for adult hippocampus neurogenesis and depression-like behaviors in PRS mice.
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http://dx.doi.org/10.1093/ijnp/pyaa020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7177164PMC
April 2020

Effects of Machining Errors on Optical Performance of Optical Aspheric Components in Ultra-Precision Diamond Turning.

Micromachines (Basel) 2020 Mar 23;11(3). Epub 2020 Mar 23.

School of Mechanical and Aerospace Engineering, Jilin University, Changchun 130022, China.

Optical aspheric components are inevitably affected by various disturbances during their precision machining, which reduces the actual machining accuracy and affects the optical performance of components. In this paper, based on the theory of multi-body system, we established a machining error model for optical aspheric surface machined by fast tool servo turning and analyzed the effect of the geometric errors on the machining accuracy of optical aspheric surface. We used the method of ray tracing to analyze the effect of the surface form distortion caused by the machining error on the optical performance, and identified the main machining errors according to the optical performance. Finally, the aspheric surface was successfully applied to the design of optical lens components for an aerial camera. Our research has a certain guiding significance for the identification and compensation of machining errors of optical components.
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http://dx.doi.org/10.3390/mi11030331DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7143373PMC
March 2020

N-Octanoyl-Dopamine inhibits cytokine production in activated T-cells and diminishes MHC-class-II expression as well as adhesion molecules in IFNγ-stimulated endothelial cells.

Sci Rep 2019 12 18;9(1):19338. Epub 2019 Dec 18.

Department of Nephrology, Endocrinology and Rheumatology, Fifth Department of Medicine, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.

IFNγ enhances allograft immunogenicity and facilitates T-cell mediated rejection. This may cause interstitial fibrosis and tubular atrophy (IFTA), contributing to chronic allograft loss. We assessed if inhibition of T-cell activation by N-octanoyl dopamine (NOD) impairs adherence of activated T-cells to endothelial cells and the ability of activated T-cells to produce IFNγ. We also assessed if NOD affects IFNγ mediated gene expression in endothelial cells. The presence of NOD during T-cell activation significantly blunted their adhesion to unstimulated and cytokine stimulated HUVEC. Supernatants of these T-cells displayed significantly lower concentrations of TNFα and IFNγ and were less capable to facilitate T-cell adhesion. In the presence of NOD VLA-4 (CD49d/CD29) and LFA-1 (CD11a/CD18) expression on T-cells was reduced. NOD treatment of IFNγ stimulated HUVEC reduced the expression of MHC class II transactivator (CIITA), of MHC class II and its associated invariant chain CD74. Since IFTA is associated with T-cell mediated rejection and IFNγ to a large extent regulates immunogenicity of allografts, our current data suggest a potential clinical use of NOD in the treatment of transplant recipients. Further in vivo studies are warranted to confirm these in vitro findings and to assess the benefit of NOD on IFTA in clinically relevant models.
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http://dx.doi.org/10.1038/s41598-019-55983-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6920350PMC
December 2019

Rational design of a novel turn-on fluorescent probe for the detection and bioimaging of hydrazine with barbituric acid as a recognition group.

Analyst 2020 Jan 2;145(2):636-642. Epub 2019 Dec 2.

Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an, Shaanxi 710127, P. R. China.

A novel turn-on fluorescent probe with barbituric acid as a unique recognition group has been rationally designed and synthesized using a facile method for detecting hydrazine. The 5-((7-(dimethylamino)-4,5-dihydronaphtho [1,2-b] thiophen-2-yl)methylene)pyrimidine-2,4,6 (1H,3H,5H)-trione (DPT) probe displays a large emission signal ratio variation (more than a 40-fold enhancement) in the presence of hydrazine under neutral conditions. Interestingly, a novel recognition mechanism based on a hydrazine-triggered addition-cyclisation-retro aldol was proposed and confirmed. Additionally, the DPT probe exhibits a low detection limit (5 × 10 M), applicable to the physiological pH range (3-12), a broad linear response range for hydrazine concentrations between 0 and 34 μM and a large Stokes shift (147 nm) for hydrazine detection in aqueous solution. Moreover, the DPT probe was successfully implemented for hydrazine imaging in vivo.
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http://dx.doi.org/10.1039/c9an02058fDOI Listing
January 2020

Postnatal Lipopolysaccharide Exposure Impairs Adult Neurogenesis and Causes Depression-like Behaviors Through Astrocytes Activation Triggering GABAA Receptor Downregulation.

Neuroscience 2019 12 1;422:21-31. Epub 2019 Nov 1.

Department of Physiology, Nanjing Medical University, Jiangsu, China. Electronic address:

Growing evidence indicates that early-life inflammation has adverse effects on adult hippocampal neurogenesis and GABA system. Based the report that hippocampal GABA system is a key modulator in adult hippocampal neurogenesis, the aim of this study was to investigate whether and how early inflammation affects GABAergic system resulting in the alterations of adult hippocampal neurogenesis and related behaviors. Neonatal mice received a daily subcutaneous injection of lipopolysaccharide (LPS, 50 μg/kg) or saline on postnatal days (PND) 3-5. Behavioral tests were used to explore LPS-induced depression-like behaviors of adult mice. Immunohistochemistry staining and western blot were employed to detect adult neurogenesis, GABAergic system, glia activation and BDNF-TrkB pathway in the hippocampus. LPS-treated mice developed a depression phenotype with the inhibited maturation of hippocampal newborn neurons in adulthood. Compared with controls, LPS mice showed a decreased expression of GABAA receptor (GABAR) protein. GABAR agonist phenobarbital could rectify the decrease of BrdU/NeuN cells in LPS mice. Additionally, postnatal LPS treatment resulted in the activation of astrocytes and the increase expression of toll-like receptor 4 (TLR4) in the second postnatal week and the downregulation of BDNF-TrkB pathway in adulthood. The treatment with TLR4 inhibitor TAK-242 restored the decrease of BrdU/NeuN cells and depression-like behaviors in LPS mice via improving GABAR. The results indicate that postnatal LPS exposure impairs adult hippocampal neurogenesis and causes depression-like behaviors through early astrocytes activation triggering the later GABAR downregulation.
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http://dx.doi.org/10.1016/j.neuroscience.2019.10.025DOI Listing
December 2019

Simultaneous determination of paracetamol and p-aminophenol using glassy carbon electrode modified with nitrogen- and sulfur- co-doped carbon dots.

Mikrochim Acta 2019 10 31;186(11):733. Epub 2019 Oct 31.

Key Laboratory of Xinjiang Phytomedicine Resources for Ministry of Education, School of Pharmacy, Shihezi University, Shihezi, 832000, China.

Walnut shell was processed for preparing nanoporous carbon, which further underwent element doping in order to boost its performance. A novel electrochemical sensor was then built by using the nitrogen and sulfur co-doped walnut shell carbon (N,S-WSC). Morphology and microstructure of the materials were characterized by scanning electron microscopy and Brunauer-Emmett-Teller (de)sorption which showed that N,S-WSC has a large specific surface with abundant pores. Electrochemical properties of differently modified sensors were investigated by cyclic voltammetry and electrochemical impedance spectroscopy. They demonstrated enhanced conductivity and enlarged surface after N,S co-doping. The modified electrode exhibits good catalytic ability towards paracetamol (ACOP) and p-aminophenol (PAP), and baseline separation of their oxidation peaks (peak potential difference is 0.24 V) allows for simultaneous detection of these two compounds. Under the optimal conditions, the calibration plot is linear in the 0.1 to 220 μM ACOP concentration range, with a 26 nM detection limit. Response to PAP is linear from 1.0 to 300 μM, and the detection limit is 38 nM (at S/N = 3). The sensor was successfully applied to quantify ACOP and PAP in tablets, and the accuracy of results is validated by HPLC. Graphical abstract Schematic representation of a novel electrochemical sensor based on N, S co-doped walnut shell carbon modified glassy carbon electrode for determination of paracetamol and p-aminophenol.
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http://dx.doi.org/10.1007/s00604-019-3870-2DOI Listing
October 2019