Publications by authors named "Ying Zhang"

8,975 Publications

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CircDLG1 promotes malignant development of non-small cell lung cancer through regulation of the miR-630/CENPF axis.

Strahlenther Onkol 2022 Jun 24. Epub 2022 Jun 24.

Department of Clinical Oncology, Shengjing Hospital of China Medical University, No. 39 Huaxiang Road, 110022, Tiexi District, Shenyang, Liaoning Province, China.

Background: Circular RNAs (circRNAs) have been reported to be crucial modulatory molecules in the etiology of non-small cell lung cancer (NSCLC). This study aimed to probe the precise role and mechanism of circRNA discs large MAGUK scaffold protein 1 (circDLG1) in the malignant progression of NSCLC.

Methods: The abundances of circDLG1, miR-630, and centromere protein F (CENPF) mRNAs were gauged by quantitative real-time polymerase chain reaction (qRT-PCR). Cell proliferation was tested in 3‑(4, 5‑dimethylthiazol-2-yl)-2, 5‑diphenyltetrazolium bromide (MTT) assay and 5‑ethynyl-2'-deoxyuridine (EdU)-incorporation assay. Cell apoptosis was analyzed by flow cytometry. Cell migration and invasion were assessed by transwell assay. Western blot was exploited to examine the levels of all proteins. The interaction between miR-630 and circDLG1 or CENPF was verified by dual-luciferase reporter, RNA pull-down, and/or RNA immunoprecipitation assays. Tumor xenograft assay and immunohistochemistry (IHC) were executed for the role of circDLG1 in tumor growth in vivo.

Results: CircDLG1 and CENPF were highly expressed in NSCLC, while miR-630 was downregulated. CircDLG1 silencing repressed proliferation, migration, and invasion, and expedited apoptosis of NSCLC cells in vitro. Mechanistically, circDLG1 deficiency modulated NSCLC cell malignant development through interacting with miR-630. Furthermore, CENPF was targeted by miR-630, and circDLG1 could positively control CENPF expression through acting as an miR-630 sponge. Furthermore, CENPF overexpression reversed the repressive impacts of circDLG1 inhibition in the malignant behaviors of NSCLC cells. Besides, circDLG1 interference hindered tumor growth in vivo.

Conclusion: CircDLG1 knockdown could impede NSCLC advancement through modulating the miR-630/CENPF axis, manifesting as a promising molecular target for NSCLC treatment.
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http://dx.doi.org/10.1007/s00066-022-01965-8DOI Listing
June 2022

Depression, religiosity, and telomere length in the Study on Stress, Spirituality, and Health (SSSH).

Int J Ment Health Addict 2022 Jun 4;20(3):1465-1484. Epub 2021 Jan 4.

MGH/Harvard Center on Genomics, Vulnerable Populations, and Health Disparities, Mongan Institute, Massachusetts General Hospital, and Harvard Medical School, Boston, MA, USA.

Prospective studies on the association between depression and telomere length have produced mixed results and have been largely limited to European ancestry populations. We examined the associations between depression and telomere length, and the modifying influence of religion and spirituality, in four cohorts, each representing a different race/ethnic population. Relative leukocyte telomere length (RTL) was measured by a quantitative polymerase chain reaction. Our result showed that depression was not associated with RTL (percent difference: 3.0 95% CI: -3.9, 10.5; p = 0.41; p-heterogeneity across studies = 0.67) overall or in cohort-specific analyses. However, in cohort-specific analyses, there was some evidence of effect modification by the extent of religiosity or spirituality, religious congregation membership, and group prayer. Further research is needed to investigate prospective associations between depression and telomere length, and the resources of resilience including dimensions of religion and spirituality that may impact such dynamics in diverse racial/ethnic populations.
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http://dx.doi.org/10.1007/s11469-020-00455-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9211376PMC
June 2022

Immunological Study of Combined Administration of SARS-CoV-2 DNA Vaccine and Inactivated Vaccine.

Vaccines (Basel) 2022 Jun 10;10(6). Epub 2022 Jun 10.

Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, China.

Objective: We constructed two DNA vaccines containing the receptor-binding domain (RBD) genes of multiple SARS-CoV-2 variants and used them in combination with inactivated vaccines in a variety of different protocols to explore potential novel immunization strategies against SARS-CoV-2 variants.

Methods: Two DNA vaccine candidates with different signal peptides (namely, secreted and membrane signal peptides) and RBD protein genes of different SARS-CoV-2 strains (Wuhan-Hu-1, B.1.351, B.1.617.2, C.37) were used. Four different combinations of DNA and inactivated vaccines were tested, namely, Group A: three doses of DNA vaccine; B: three doses of DNA vaccine and one dose of inactivated vaccine; C: two doses of inactivated vaccine and one dose of DNA vaccine; and D: coadministration of DNA and inactivated vaccines in two doses. Subgroups were grouped according to the signal peptide used (subgroup 1 contained secreted signal peptides, and subgroup 2 contained membrane signal peptides). The in vitro expression of the DNA vaccines, the humoral and cellular immunity responses of the immunized mice, the immune cell population changes in local lymph nodes, and proinflammatory cytokine levels in serum samples were evaluated.

Results: The antibody responses and cellular immunity in Group A were weak for all SARS-CoV-2 strains; for Group B, there was a great enhancement of neutralizing antibody (Nab) titers against the B.1.617.2 variant strain. Group C showed a significant increase in antibody responses (NAb titers against the Wuhan-Hu-1 strain were 768 and 1154 for Group C1 and Group C2, respectively, versus 576) and cellular immune responses, especially for variant B.1.617.2 (3240 ( < 0.001) and 2430 ( < 0.05) for Group C1 and Group C2, versus 450); Group D showed an improvement in immunogenicity. Group C induced higher levels of multiple cytokines.

Conclusion: The DNA vaccine candidates we constructed, administered as boosters, could enhance the humoral and cellular immune responses of inactivated vaccines against COVID-19, especially for B.1.617.2.
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http://dx.doi.org/10.3390/vaccines10060929DOI Listing
June 2022

Vibration Characteristics of a Laminated Composite Double-Cylindrical Shell System Coupled with a Variable Number of Annular Plates.

Materials (Basel) 2022 Jun 15;15(12). Epub 2022 Jun 15.

College of Mechanical and Electrical Engineering, Harbin Engineering University, Harbin 150001, China.

A vibration characteristic analysis model of a laminated composite double cylindrical shell system (LCDCSS) coupled with several annular plates under general boundary conditions is established. Artificial springs are used to simulate the coupling relationship between substructures to ensure the continuity of displacement both at ends of the shells and coupling boundaries. The variable number of annular plates can be distributed unevenly and coupled elastically. Displacement functions of LCDCSS are expressed with improved Fourier series. Based on the principle of energy, obtain the unknown coefficients of the displacement components by using the Rayleigh-Ritz method. The convergence and effectiveness of the proposed method are verified by comparing with the results with literature and FEM, and then carried out parametric investigation to study the free and steady-state response vibration characteristics of LCDCSS. Rapid prediction of free vibration and response vibration of a double-layer cylindrical shell system with various structures and scales is realized by exploiting the model, and some new results of double-layer cylindrical shell system are explored, which can provide reference for further research.
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http://dx.doi.org/10.3390/ma15124246DOI Listing
June 2022

Inhibitory Mechanism of Advanced Glycation End-Product Formation by Avenanthramides Derived from Oats through Scavenging the Intermediates.

Foods 2022 Jun 20;11(12). Epub 2022 Jun 20.

School of Food and Health, Beijing Technology and Business University, Beijing 100048, China.

As a special polyphenolic compound in oats, the physiological function of oat avenanthramides (AVAs) drives a variety of biological activities, and plays an important role in the prevention and treatment of common chronic diseases. In this study, the optimum extraction conditions and structural identification of AVAs from oats was studied. The inhibitory effect of AVAs from oats on advanced glycation end-products (AGEs) in a glucose-casein simulation system was evaluated, and this revealed dose-dependent inhibitory effects. The trapping capacity of AVAs to the α-dicarbonyl compounds of AGE intermediate products was determined by HPLC-MS/MS, and the results indicate that AVA 2c, AVA 2p, and AVA 2f exhibited the ability to capture -dicarbonyl compounds. More importantly, AVA 2f was found to be more efficient than AVA 2p at inhibiting superoxide anion radical (O), hydroxyl radical (OH), and singlet oxygen (O) radical generation, which may be the main reason that AVA 2f was more efficient than AVA 2p in AGE inhibition. Thus, this research presents a promising application of AVAs from oats in inhibiting the food-borne AGEs formed in food processing.
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http://dx.doi.org/10.3390/foods11121813DOI Listing
June 2022

Super-Enhancers, Phase-Separated Condensates, and 3D Genome Organization in Cancer.

Cancers (Basel) 2022 Jun 10;14(12). Epub 2022 Jun 10.

Cancer Science Institute of Singapore, National University of Singapore, Singapore 117599, Singapore.

3D chromatin organization plays an important role in transcription regulation and gene expression. The 3D genome is highly maintained by several architectural proteins, such as CTCF, Yin Yang 1, and cohesin complex. This structural organization brings regulatory DNA elements in close proximity to their target promoters. In this review, we discuss the 3D chromatin organization of super-enhancers and their relationship to phase-separated condensates. Super-enhancers are large clusters of DNA elements. They can physically contact with their target promoters by chromatin looping during transcription. Multiple transcription factors can bind to enhancer and promoter sequences and recruit a complex array of transcriptional co-activators and RNA polymerase II to effect transcriptional activation. Phase-separated condensates of transcription factors and transcriptional co-activators have been implicated in assembling the transcription machinery at particular enhancers. Cancer cells can hijack super-enhancers to drive oncogenic transcription to promote cell survival and proliferation. These dysregulated transcriptional programs can cause cancer cells to become highly dependent on transcriptional regulators, such as Mediator and BRD4. Moreover, the expression of oncogenes that are driven by super-enhancers is sensitive to transcriptional perturbation and often occurs in phase-separated condensates, supporting therapeutic rationales of targeting SE components, 3D genome organization, or dysregulated condensates in cancer.
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http://dx.doi.org/10.3390/cancers14122866DOI Listing
June 2022

MicroRNA-23a-3p promotes macrophage M1 polarization and aggravates lipopolysaccharide-induced acute lung injury by regulating PLK1/STAT1/STAT3 signalling.

Int J Exp Pathol 2022 Jun 23. Epub 2022 Jun 23.

Department of Emergency, Tiantai Hospital of Hangzhou Medical College, Tiantai, China.

Macrophage polarization is an important effectorprocess in acute lung injury (ALI) induced by sepsis. MicroRNAs (miRNAs) have emerged as important players in regulating ALI process. Here, we showed that elevated microRNA-23a-3p (miR-23a-3p) promoted LPS-induced macrophage polarization and ALI in mice, while inhibition of miR-23a-3p led to reduced macrophage response and ameliorated ALI inflammation. Mechanically, miR-23a-3p regulated macrophage M1 polarization through targeting polo-like kinase 1 (PLK1). PLK1 was downregulated in LPS-treated macrophages and ALI mouse lung tissues. Knockdown of PLK1 increased macrophage M1 polarization through promoting STAT1/STAT3 activation, while overexpression of PLK1 reduced macrophage immune response. Collectively, our results reveal a key miRNA regulon that regulates macrophage polarization for LPS-induced immune response.
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http://dx.doi.org/10.1111/iep.12445DOI Listing
June 2022

PeTGA1 enhances disease resistance against Colletotrichum gloeosporioides through directly regulating PeSARD1 in poplar.

Int J Biol Macromol 2022 Jun 20. Epub 2022 Jun 20.

National Engineering Research Center of Tree breeding and Ecological remediation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. Electronic address:

Basic leucine zipper (bZIP) proteins play important roles in responding to biotic and abiotic stresses in plants. However, the molecular mechanisms of plant resistance to pathogens remain largely unclear in poplar. The present study isolated a TGACG-binding (TGA) transcription factor, PeTGA1, from Populus euphratica. PeTGA1 belongs to subgroup D of the bZIP family and was localized to the nucleus. To study the role PeTGA1 plays in response to Colletotrichum gloeosporioides, transgenic triploid white poplars overexpressing PeTGA1 were generated. Results showed that poplars with overexpressed PeTGA1 showed a higher effective defense response to C. gloeosporioides than the wild-type plants. A yeast one-hybrid assay and an electrophoretic mobility shift assay revealed that PeTGA1 could directly bind to the PeSARD1 (P. euphratica SYSTEMIC ACQUIRED RESISTANCE DEFICIENT 1) promoter, an important regulator for salicylic acid biosynthesis. The transactivation assays indicated that PeTGA1 activated the expression of PeSARD1, and PR1 (PATHOGENESIS-RELATED 1), a SA marker gene involved in SA signaling. Subsequently, we observed that the PeTGA1 overexpression lines showed elevated SA levels, thereby resulting in the increased resistance to C. gloeosporioides. Taken together, our results indicated that PeTGA1 may exert a key role in plant immunity not only by targeting PeSARD1 thus participating in the SA biosynthesis pathway but also by involving in SA signaling via activating the expression of PR1.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.06.099DOI Listing
June 2022

Analyses of n-back high-load working memory in early Parkinson's disease: An fMRI study.

J Clin Neurosci 2022 Jun 20;102:80-86. Epub 2022 Jun 20.

Department of Radiology, The First Hospital of Jilin University, Changchun, Jilin, China. Electronic address:

Background: Cognitive impairment is common in Parkinson's disease (PD). Working memory is a group of basic processes that is part of many cognitive operations (from complex decision-making to selective attention). To date, there is not sufficient research on working memory impairment in early PD patients, especially patients under high load.

Aim: The aim of this study was to observe the activation of brain regions in early PD patients during verbal high-load cognitive tasks and to explore the correlation between cognitive behavior indexes and gray matter areas of brain structure.

Methods: A total of 45 participants were included: 15 healthy controls and 30 PD patients without dementia. They underwent BOLD imaging during a digital N-back working memory task in a 3 T scanner, and their high-resolution T1-weighted three-dimensional brain anatomical images were collected. The SPM12 standard method was used to generate a comparison map with 2 plus 3-back greater than 0-back, and with age, sex, levodopa equivalent daily dose (LEDD) and education as covariables, and multiple regression analysis was conducted to analyze the correlation between MOCA, MES and brain gray matter volume.

Results: Compared with the control group, the frontoparietal region was significantly activated in patients with PD under the background of high working memory burden. In addition, there was a widespread area correlation between the brain structure and basic cognitive function.

Conclusions: The frontoparietal cortex is the core hub of working memory, and there may be a compensation mechanism in PD patients without dementia with a high-load of working memory.
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http://dx.doi.org/10.1016/j.jocn.2022.06.010DOI Listing
June 2022

The Promoter Analysis of Gene: A Candidate Gene Identified through Transcriptional Profiling of Methyl Jasmonate Treated Grapevine ( L.).

Plants (Basel) 2022 Jun 9;11(12). Epub 2022 Jun 9.

Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, China.

Methyl jasmonate (MeJA) plays a vital role in plant disease resistance and also induces the expression of disease resistance genes in plants. In this study, a transcriptome analysis was performed on grapevine leaves after 12, 24 and 48 h of MeJA-100 μM treatment. A total of 1242 differentially expressed genes (DEGs) were identified from the transcriptome data, and the analysis of the DEGs showed that genes related to phytohormone signal transduction, jasmonic acid-mediated defense, Mitogen-activated protein kinase (MAPK), and flavonoid biosynthetic pathways were upregulated. As Pathogenesis-related gene 1 (PR1) is an important marker gene in plant defense also upregulated by MeJA treatment in RNA-seq data, the gene was selected for a promoter analysis with β-glucuronidase (GUS) through transient expression in tobacco leaves against abiotic stress. The results showed that the region from -1837 bp to -558 bp of the promoter is the key region in response to hormone and wound stress. In this study, we extended the available knowledge about induced defense by MeJA in a grapevine species that is susceptible to different diseases and identified the molecular mechanisms by which this defense might be mediated.
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http://dx.doi.org/10.3390/plants11121540DOI Listing
June 2022

Overexpression Pattern of miR-301b in Osteosarcoma and Its Relevance with Osteosarcoma Cellular Behaviors via Modulating SNX10.

Biochem Genet 2022 Jun 22. Epub 2022 Jun 22.

Department of Oncology & Vascular Intervention Radiology, Zhongshan Hospital of Xiamen University, School of Medicine, Xiamen University, No. 201 HuBinNan Road, Xiamen, 361000, China.

Prior studies have noted the importance of microRNAs (miRNAs) in development and progression of osteosarcoma (OS), but the influence of miR-301b is less investigated. This investigation aimed to explore the biological role of miR-301b/SNX10 in OS. GSE28423 and GSE28424 arrays delivered the corresponding miR-301b and sorting nexin 10 (SNX10) expression levels in OS samples. miR-301b and SNX10 expressions were also measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and western blotting in cells. Cell counting kit (CCK)-8 and transwell analysis were applied to measure cell characteristics. Luciferase reporter assay and Pearson correlation analysis were used to detect the relevance between miR-301b and SNX10. miR-301b was extremely increased in OS tissues compared with normal tissues, while SNX10 was decreased. The proliferation, invasion, and migration capabilities were limited following a low expression level of miR-301b whereas miR-301b overexpression promoted cellular malignant behaviors. miR-301b negatively targeted SNX10. The elevated SNX10 expression highlighted the inhibitory function on cell proliferation, migration, and invasion in OS cells treated by miR-301b inhibitor. Reduction of miR-301b induced the decrease of epithelial-mesenchymal transition (EMT)-related markers including N-cadherin, Vimentin, and matrix metallo-proteinase 9 (MMP)9. These results are added to the complete expanding field of the potential effects of miR-301b in OS cell malignant behaviors and demonstrate its promising role for further use to treat human OS.
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http://dx.doi.org/10.1007/s10528-022-10241-4DOI Listing
June 2022

Radical Brook Rearrangement: Concept and Recent Developments.

Angew Chem Int Ed Engl 2022 Jun 21. Epub 2022 Jun 21.

ShanghaiTech University, School of Physical Science and Technology, 393 Middle Huaxia Road, Pudong, 201210, Shanghai, CHINA.

Brook rearrangement has already established as one of the important molecular rearrangements in synthetic chemistry and been applied in the complex generation, drug discovery, material science and natural products synthesis. Comparing to its widely known ionic mechanism, radical Brook rearrangement is less explored due to the difficult generation of alkoxyl radical species. This minireview will summarize the early development and general concept of radical Brook rearrangement, highlight the recent advances in photocatalytic reactions and transition-metal catalysed cross-couplings involving radical Brook rearrangement. Furthermore, we hope this survey will inspire further development in this emerging area.
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http://dx.doi.org/10.1002/anie.202205671DOI Listing
June 2022

Effects of Selenoprotein S Knockdown on Endoplasmic Reticulum Stress in ATDC5 Cells and Gene Expression Profiles in Hypertrophic Chondrocytes.

Biol Trace Elem Res 2022 Jun 20. Epub 2022 Jun 20.

Key Laboratory of Environment and Genes Related to Diseases in the Education Ministry and Key Laboratory of Trace Elements and Endemic Diseases in Ministry of Health, School of Public Health, Xi'an Jiaotong University, Xi'an, Shaanxi, China.

Selenoprotein S (SelS), a member of the selenoprotein family, is mainly located on the endoplasmic reticulum (ER) membrane. SelS is involved in a variety of biological processes, including oxidative stress, inflammation, glucose metabolism regulation, and ER-associated protein degradation (ERAD). This study was designed to explore the role of SelS in chondrocytes. It was confirmed that SelS is a Se-sensitive selenoprotein in low-selenium rat and cell models. ER stress was not induced in SelS knockdown ATDC5 cells. However, treatment of ATDC5 cells with tunicamycin (Tm), an ER stress inducer, increased the expression of SelS, and knockdown of SelS aggravated ER stress induced by Tm, suggesting that SelS is a regulatory molecule involved in ER stress in chondrocytes. Both osteoarthritis and Kashin-Beck disease are osteochondral diseases associated with hypertrophic chondrocyte abnormalities. Therefore, ATDC5 cells were induced to hypertrophic chondrocytes. SelS was knocked down and RNA sequencing was performed. Bioinformatics analysis of the differentially expressed genes (DEGs) revealed that SelS knockdown affected a variety of biological processes, including cell adhesion, osteoclast differentiation, and extracellular matrix homeostasis. Collectively, this study verified that SelS is sensitive to selenium levels and is an ER stress-responsive molecule. Knocking down SelS can cause abnormal expression of adhesion molecules and matrix homeostasis disorder in hypertrophic chondrocytes.
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http://dx.doi.org/10.1007/s12011-022-03313-4DOI Listing
June 2022

A photochemical-responsive nanoparticle boosts doxorubicin uptake to suppress breast cancer cell proliferation by apoptosis.

Sci Rep 2022 Jun 20;12(1):10354. Epub 2022 Jun 20.

Department of Rehabilitation Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.

In the course of chemotherapy for breast cancer, doxorubicin (DOX) is one of the most commonly prescribed agents. However, it has been recognized as clinically circumscribed on account of its poor selectivity and toxic reactions to normal tissues. Fortunately, the distinct merit of photochemical-responsive nanoparticle delivery systems to enhance cellular drugs uptake through localized concentration, adequate selective and minimizing systemic toxicity has aroused substantial interest recently. In this study, we synthesized photochemical-responsive nanoparticle by incorporating DOX, curcumin (CUR), and perfluorooctyl bromide (PFOB) into poly(lactic-co-glycolic acid) (PLGA) via double emulsification (DOX-CUR-PFOB-PLGA). The synthesized composite nanoparticles, which featured good ultrasound imaging, engendered photochemical activation for drug release when given laser irradiation. Cumulative release rates for DOX were 76.34%, and for CUR were 83.64%, respectively. Also, MCF-7 cells displayed significant intracellular DOX uptake and reactive oxygen species (ROS) levels, degraded cytoskeleton, and decreased cell growth and migration capacity. At the molecular level, cellular pAKT levels decreased, which resulted in downregulated HIF-1α and BAX/BCl-2 levels, leading to Caspase-3 activation and thus induction of apoptosis. Therefore, the photochemical-responsive nanoparticles possess the potential to elicit apoptosis in MCF-7 cells via enhanced DOX uptake.
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http://dx.doi.org/10.1038/s41598-022-14518-xDOI Listing
June 2022

pH-Responsive Regulation of a Surfactant-Free Microemulsion Based on Hydrophobic Deep Eutectic Solvents.

Langmuir 2022 Jun 18. Epub 2022 Jun 18.

School of New Energy and Materials, Southwest Petroleum University, Chengdu 610500, P. R. China.

Microemulsions containing a responsive hydrophobic deep eutectic solvent (HDES) as the oil phase that can replace conventional organic solvents are considered to be a green strategy. It is anticipated that a pH-responsive HDES is synthesized to prepare rapid responsive surfactant-free microemulsions (SFMEs), which enable the transition from SFMEs to nanoemulsions. Menthol and -octanoic acid (OA) were assembled into HDES by hydrogen bonding at a molar ratio of 1:2. The pH-responsive HDES as the oil phase and isopropyl alcohol (IPA) as the double solvent could form HDES/IPA/water SFMEs, which have unique responsiveness. Specifically, from the nuclear magnetic resonance hydrogen spectrum, pH, thermogravimetry, and Fourier transform infrared spectroscopy investigations, the excellent switchability and stability of menthol-OA were demonstrated. On the basis of these complexes, microemulsions were successfully prepared. Electrical conductivity and pH measurements were used to determine the structures of microemulsions and the phase inversion process. The effects of the contents of water and HDES, NaCl concentration, and pH of the system were investigated. Nanoemulsions were successfully prepared on the basis of the pH response of the microemulsions. In addition, the prepared nanoemulsion has a unique pH-responsive behavior that can be controllably regulated among nanoemulsions, microemulsions, and phase separation systems.
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http://dx.doi.org/10.1021/acs.langmuir.2c00181DOI Listing
June 2022

Phillyrin Prevents Ovariectomy-Induced Osteolysis by Inhibiting Osteoclast Differentiation.

Evid Based Complement Alternat Med 2022 10;2022:6065494. Epub 2022 Jun 10.

Department of Orthopedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Postmenopausal osteoporosis is a metabolic disease caused by an imbalance between osteoclasts and osteoblasts. At present, the drug strategy for treating postmenopausal osteoporosis has some limitations and is unable to satisfy the demands of patients. is an herbal extract from , with an inhibitory effect on osteolysis. In this study, we described the role of in ovariectomy-induced osteoporosis and its effect on osteoclast differentiation . Eighteen female C57BL/6 mice were randomly divided into three groups: sham group (sham surgery and injection with 0.9% normal saline), ovariectomized group (ovariectomy and injection with 0.9% normal saline), and group (ovariectomy and injecting with 100 mg/kg for 2 days). Mice were sacrificed after 6-week administration and femurs were harvested for microcomputed tomography (micro-CT) and histomorphology analyses. In vitro, we used different concentrations of to study its effect on osteoclastogenesis. The results showed that the BV/TV, Tb.Th, and Tb.N in trabecular bone were increased in the group compared with the OVX group, and the trabecular bone mass was remarkably decreased in the OVX group compared with the sham group. The number of osteoclasts was increased in the OVX group compared to the sham group, and the number and area of osteoclasts were decreased in the group compared to the control group. Compared with the OVX group, the number and area of osteoclasts were reduced in the group. In conclusion, could inhibit the formation of osteoclasts, promote the growth of bone trabecular, and relieve osteoporosis caused by ovariectomy, with a certain clinical adoption value.
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http://dx.doi.org/10.1155/2022/6065494DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9205725PMC
June 2022

PAP Polypeptide Promotes Osteogenesis in Jaw Bone Defect Repair by Inhibiting Inflammatory Reactions.

Front Bioeng Biotechnol 2022 2;10:916330. Epub 2022 Jun 2.

Department of Stomatology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Jaw defects are common in oral and maxillofacial diseases and require surgical repair in extreme cases. Given the limitations in availability and efficacy of autologous bone grafts or allografts, great effort has been made in finding suitable, biocompatible, and effective artificial bone materials. Considering the key role of inflammation in bone resorption, we sought to identify a polypeptide with anti-inflammatory and bone-promoting effects. Rat bone marrow-derived mesenchymal cells (BMSCs) were treated with lipopolysaccharide (LPS) to induce an inflammatory environment, and 1,538 differentially abundant polypeptides were identified using mass spectrometry. Based on mass spectrometry signal intensity, multiple of difference, and structural stability, PAP was screened out as the polypeptide with the lowest abundance in the inflammatory condition. PAP showed no cytotoxicity to BMSCs with increasing concentrations. PAP (10 μM) also increased alkaline phosphatase activity and mRNA expression of , , and in a concentration-dependent manner, which confirmed that it can promote osteogenic induction of rat BMSCs. Moreover, PAP reduced LPS-induced expression of inflammatory cytokines (TNF-α, IL-1β, IL-6) and reactive oxygen species and inhibited polarization of RAW 264.7 macrophages to the inflammatory type. Finally, a skull defect mouse model was established, and mice were injected with LPS and/or PAP. Micro-CT, histological analysis, and immunohistochemical staining showed that PAP significantly reduced the number of LPS-induced bone resorption pits and maintained bone integrity. Overall, the polypeptide PAP screened using LPS stimulation of BMSCs is not cytotoxic and can inhibit the inflammatory reaction process to promote osteogenesis. This study thus provides a basis for development of PAP as a new osteogenic material in the repair of jaw defects.
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http://dx.doi.org/10.3389/fbioe.2022.916330DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201685PMC
June 2022

Contrast Agents for Hepatocellular Carcinoma Imaging: Value and Progression.

Front Oncol 2022 2;12:921667. Epub 2022 Jun 2.

Department of Gastroenterology, Graduate School of Medicine, Yokohama City University, Yokohama, Japan.

Hepatocellular carcinoma (HCC) has the third-highest incidence in cancers and has become one of the leading threats to cancer death. With the research on the etiological reasons for cirrhosis and HCC, early diagnosis has been placed great hope to form a favorable prognosis. Non-invasive medical imaging, including the associated contrast media (CM)-based enhancement scan, is taking charge of early diagnosis as mainstream. Meanwhile, it is notable that various CM with different advantages are playing an important role in the different imaging modalities, or even combined modalities. For both physicians and radiologists, it is necessary to know more about the proper imaging approach, along with the characteristic CM, for HCC diagnosis and treatment. Therefore, a summarized navigating map of CM commonly used in the clinic, along with ongoing work of agent research and potential seeded agents in the future, could be a needed practicable aid for HCC diagnosis and prognosis.
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http://dx.doi.org/10.3389/fonc.2022.921667DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200965PMC
June 2022

A difficult-to-diagnose pericardial fistula.

Am J Med Sci 2022 Jun 16. Epub 2022 Jun 16.

Department of Oncology, Changzhi People's Hospital, The Affiliated Hospital of Changzhi Medical College, Changzhi, Shanxi 046000, China. Electronic address:

Pericardial fistula is a rare complication. Generally, the diagnosis can be confirmed by imaging examination, but our patient was an exception. We present a 71-year-old female patient that complained of remnant gastric cancer for five months and dyspnea for seven days; the dyspnea became aggravated during the last two days. After admission, emergency thoracic computed tomography and echocardiography showed pericardial effusion, and pericardiocentesis was performed. After conventional treatment, the pericardial effusion was unchanged and no cancer cells were found in the pericardial drainage. However, the color changed from turbid to golden yellow and, finally, to green. After 20 days of repeated laboratory, imaging, and gastrointestinal contrast examinations, no cause was found. Moreover, a clinical diagnosis could not be obtained following numerous comprehensive clinical analyses. Given the color change of the pericardial drainage, we strongly suspected pericardial fistula, but the imaging examinations were negative. Finally, a methylene blue test confirmed the existence of a pericardial fistula. When the color of the pericardial effusion changes, the existence of a pericardial fistula must be considered in advance, and other methods should be evaluated if imaging cannot assist in the diagnosis.
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http://dx.doi.org/10.1016/j.amjms.2022.06.008DOI Listing
June 2022

Discovery of a potent and selective proteolysis targeting chimera (PROTAC) degrader of NSD3 histone methyltransferase.

Eur J Med Chem 2022 Jun 13;239:114528. Epub 2022 Jun 13.

Department of Medicinal Chemistry, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 ZuChongZhi Road, Shanghai, 201203, PR China; University of Chinese Academy of Sciences, NO. 19A Yuquan Road, Beijing, 100049, PR China; School of Chinese Materia Medica, Nanjing University of Chinese Medicine, Qixia District, Nanjing, 210023, PR China. Electronic address:

Nuclear receptor binding SET domain protein 3 (NSD3) is an attractive potential target in the therapy for human cancers. Herein, we report the discovery of a series of small-molecule NSD3 degraders based on the proteolysis targeting chimera (PROTAC) strategy. The represented compound 8 induces NSD3 degradation with DC values of 1.43 and 0.94 μM in NCI-H1703 and A549 lung cancer cells, respectively, and shows selectivity over two other NSD proteins. 8 reduces histone H3 lysine 36 methylation and induces apoptosis and cell cycle arrest in lung cancer cells. Moreover, the RNA sequencing and immunohistochemistry assays showed that 8 downregulates NSD3-associated gene expression. Significantly, 8, but not 1 (a reported NSD3-PWWP antagonist) could inhibit the cell growth of NCI-H1703 and A549 cells. A single administration of 8 effectively decreases the NSD3 protein level in lung cancer xenograft models. Therefore, this study demonstrated that inducing NSD3 degradation is a more effective approach inhibiting the function of NSD3 than blocking the NSD3-PWWP domain, which may provide a potential therapeutic approach for lung cancer.
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http://dx.doi.org/10.1016/j.ejmech.2022.114528DOI Listing
June 2022

Ultrahigh sensitivity nitrogen-doping carbon nanotubes-based metamaterial-free flexible terahertz sensing platform for insecticides detection.

Food Chem 2022 Jun 11;394:133467. Epub 2022 Jun 11.

Beijing Technology and Business University (BTBU), No. 11 Fucheng Road, Beijing 100048, People's Republic of China.

With the rapid advances in terahertz (THz) spectroscopy, metamaterial-free THz sensors have been of importance due to efficient cost, high sensitivity and overcoming the limited tunability of the optical constants of metals. Here, a metamaterial-free and flexible THz sensor based on nitrogen-doping carbon nanotubes (N-CNTs) coupled with signal-enhancing Au NPs was proposed for detecting nereistoxin-related insecticides (NRIs). Sensitivity and selectivity for NRIs detection have been realized over the range of 3.3-100 μg/L with good linear fitting (R ≥ 0.9003) and LOD was 1.33 μg/L. Accuracy was validated by the recovery rates of 105.87-109.75% of NRI in spiked food-matrix sample. These results indicated the developed signal-enhancing THz method, validated by LC-MS/MS, exhibited high sensitivity and simplicity detection, which has noteworthy potential for applications in food safety and environment monitoring.
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http://dx.doi.org/10.1016/j.foodchem.2022.133467DOI Listing
June 2022

Inhibitory mechanism of tangeretin, a citrus flavone on the sphingosylphosphorylcholine (SPC)-induced vascular smooth muscle contraction.

J Pharmacol Sci 2022 Aug 13;149(4):189-197. Epub 2022 May 13.

Department of Advanced Preventive Medicine, School of Medicine, Yamaguchi University, 1-1-1 Minami-Kogushi, Ube, 755-8505, Japan. Electronic address:

We previously discovered that the SPC/Fyn/Rho-kinase (ROK) pathway mediates the Ca-sensitization of coronary arterial smooth muscle (CASM) contraction leading to vasospasm, a major cause of sudden death. Lately, we have been trying to find and develop more natural edible compounds which can treat and/or prevent the SPC-induced abnormal CASM contraction, and finally the first to discover that tangeretin (5,6,7,8,4'-pentamethoxyflavone), a natural compound extracted from citrus plants, can inhibit the SPC-induced CASM contraction both in the pretreatment and posttreatment. In porcine CASM tissues, tangeretin showed remarkable inhibitory effects on the SPC-induced contraction with modest inhibitory effects on the high K-depolarization-induced Ca-dependent contraction, both in pretreatment and posttreatment at the optimal concentrations; Regarding the mechanisms, tangeretin markedly abolished the SPC-induced cell contraction through inhibiting the SPC-induced activation and translocation of Fyn and ROK from the cytoplasm to the cell membrane in cultured CASM cells, resulting in the reduction of phosphorylation of myosin light chain. Taken together, these findings indicate that tangeretin, upon pre- or post-treatment, inhibits the SPC-induced CASM contraction through suppressing the Fyn/ROK signaling pathway, thereby suggesting that tangeretin can be a potential candidate for the treatment and/or prevention of vasospasm.
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http://dx.doi.org/10.1016/j.jphs.2022.05.002DOI Listing
August 2022

Ammonia mitigation potential in an optimized crop-layer production system.

Sci Total Environ 2022 Jun 15;841:156701. Epub 2022 Jun 15.

National Academy of Agriculture Green Development, China Agricultural University, Beijing 100193, China; College of Resources and Environmental Sciences, Key Laboratory of Plant-Soil Interactions of MOE, China Agricultural University, Beijing 100193, China.

Livestock and crop production are the main sources of ammonia (NH) emissions, which are known to degrade the air quality. Numerous studies have been conducted to explore the mitigation potential of various approaches, although few have examined the systematic NH emission mitigation potential when considering both crop and livestock systems based on coherent in situ measurement results. Herein, we design an optimal system wherein coupled crop and layer production systems reveal feasible approaches for significant mitigation potential at each stage of the process. Specifically, these measures involve (i) using a low crude protein (LCP) feed, (ii) composting manure with certain additives, and (iii) substituting manure with optimal fertilization in a summer maize-winter wheat cropping system. The results show that (i) LCP feed leads to a 14 % reduction in NH emissions at the housing stage, (ii) introducing additives during the composing stage reduces NH emissions by 16 %-46 %, and (iii) the NH reduction potential reaches 35 %-44 % at the field application stage. In the overall crop-layer system, the optimal system with the improved management strategy applied at every stage results in a 48 % and 56 % reduction in NH emissions for per unit eggs and grain production, respectively, relative to a traditional production system. This study confirms that NH emissions can be cut in half by implementing optimal crop-livestock systems with appropriate mitigation approaches. This is a feasible model that can be promoted and extended in various agricultural areas, which together with technological, policy, and economic support can enable significant mitigation potential for sustainable agriculture development.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156701DOI Listing
June 2022

A retrospective study of the role of preoperative ultrasonography in the detection of deep vein thrombosis in 1750 patients with gastric and colorectal cancers.

Thromb Res 2022 Jun 13;216:52-58. Epub 2022 Jun 13.

Department of Gastrointestinal Surgery, Xijing Hospital, Air Force Military Medical University, Xi'an, Shaanxi, China. Electronic address:

Background: There has been a lack of research in the past on the prevalence and risk factors associated with deep vein thrombosis (DVT) in patients with resectable gastric and colorectal cancers. The purpose of this study was to review the anatomical distribution, prevalence and risk factors associated with lower limb DVT in 1750 patients with preoperative gastric and colorectal cancers and to evaluate the role of preoperative ultrasonography in the detection of DVT in preventing postoperative pulmonary thromboembolism (PTE) in patients with gastric and colorectal cancers.

Methods: A total of 1750 patients with gastric and colorectal cancers who underwent preoperative venous ultrasonography of the lower limbs were retrospectively reviewed. The risk factors associated with preoperative DVT were identified using univariate and multivariate logistic regression analysis.

Results: Seventy-three of the 1750 patients with gastric and colorectal cancers had DVT detected by preoperative venous ultrasonography of the lower limb and the incidence of lower limb DVT was 4.17 % in 1750 patients with gastric and colorectal cancers. Univariate analysis showed a higher risk of DVT in patients who met the following criteria: aged ≥80 years, female sex, the performance status ≥1, stage IV, ASA class ≥ III/IV, and hypertension. Multivariate logistic regression analysis showed that female sex, stage IV and ASA class ≥ III/IV were significantly associated with DVT before gastric and colorectal cancer surgery.

Conclusions: Our study showed that female sex, stage IV and ASA class ≥ III/IV were significantly associated with DVT before gastric and colorectal cancer surgery. Routine venous ultrasonography for the lower limb can identify the risk of PTE, which is of great significance in the prevention and occurrence of PTE.
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http://dx.doi.org/10.1016/j.thromres.2022.06.003DOI Listing
June 2022

NRF2 Genetic Polymorphism Modifies the Association of Plasma Selenium Levels With Incident Coronary Heart Disease among Type 2 Diabetes Individuals.

Diabetes 2022 Jun 17. Epub 2022 Jun 17.

Department of Occupational and Environmental Health, Ministry of Education and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Existing studies found that both plasma selenium and NRF2 promoter variants (e.g., rs6721961) were associated with cardiovascular disease risk in the general population. However, epidemiological evidence on the interaction between plasma selenium and NRF2 genetic susceptibility in relation to incident coronary heart disease (CHD) risk remains scarce, especially among individuals with type 2 diabetes (T2D). Thus, we examined whether rs6721961 in the NRF2 gene might modify the association between plasma selenium levels and incident CHD risk among T2D. During a mean (SD) follow-up period of 6.90 (2.96) years, 798 incident CHD cases were identified in 2,251 T2D cases. Risk allele carriers (GT/TT) of rs6721961 showed a higher risk of incident CHD among T2D (adjusted HR = 1.17, 95% CI: 1.02-1.35) vs. non-risk allele carriers (GG). Each 22.8 μg/L increase in plasma selenium levels was associated with a reduced risk of incident CHD among T2D with risk allele (GT/TT; HR = 0.80, 95% CI: 0.71-0.89), whereas no association was found in those with non-risk allele (GG; Pinteraction = 0.004), indicating that NRF2 promoter polymorphism might modify the association between plasma selenium levels and incident CHD risk among T2D. Our study suggests that redox-related genetic variants should be considered to identify populations that might benefit most from selenium supplementation. Further mechanistic studies are warranted.
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http://dx.doi.org/10.2337/db21-1124DOI Listing
June 2022

Inflammatory-Dependent Bidirectional Effect of Bile Acids on NLRP3 Inflammasome and Its Role in Ameliorating CPT-11-Induced Colitis.

Front Pharmacol 2022 31;13:677738. Epub 2022 May 31.

Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education), State Key Laboratory of Natural Medicine, China Pharmaceutical University, Nanjing, China.

Irinotecan (CPT-11) in combination with 5-fluorouracil and leucovorin is a first-line chemotherapy regimen for the treatment of colorectal cancer; however, its clinical application is limited by the dose-limiting gastrointestinal toxicity of colitis. In our previous studies, several bile acids (BAs) were found significantly elevated in the colon of the CPT-11-induced rat colitis model. On the other hand, NLRP3 inflammasome has been reported to play important roles in mediating colitis. Interestingly, BA was stated to activate the NLRP3 inflammasome in some studies, while in some other reports, it showed an inhibitory effect. We assumed that the inflammatory status in different circumstances might have contributed to the controversial findings. In this study, we first discovered, under non-inflammatory conditions, that supplementing BA could activate the NLRP3 inflammasome in THP-1-differentiated macrophages and promote inflammation. In lipopolysaccharide (LPS)-induced inflammatory macrophages, however, BA inhibited the NLRP3 inflammasome and reduced inflammation. Further experiments demonstrated that Takeda G protein-coupled receptor 5 (TGR5) is essential in mediating the inhibitory effect of BA, while phospho-SP1 (p-SP1) is key to the activation. Furthermore, we applied the above findings to ameliorate CPT-11-caused colitis in rats by inhibiting SP1 with mithramycin A (MitA) or activating TGR5 using oleanolic acid (OA). Our findings may shed light on the discovery of effective interventions for reducing dose-limiting chemotherapy-induced colitis.
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http://dx.doi.org/10.3389/fphar.2022.677738DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9193974PMC
May 2022

Prevalence and Correlates of Risky Drinking Among the Oldest-Old in China: A National Community-Based Survey.

Front Psychiatry 2022 30;13:919888. Epub 2022 May 30.

NHC Key Laboratory of Mental Health (Peking University), National Clinical Research Center for Mental Disorders (Peking University Sixth Hospital), Peking University Institute of Mental Health (Sixth Hospital), Beijing, China.

Aims: To investigate the prevalence and correlates of risky drinking in Chinese elderly people aged 80 and over.

Methods: Data were obtained from the Chinese Longitudinal Healthy Longevity Survey (CLHLS) conducted in 2018. A total of 10,141 respondents aged 80 years or older were included in this analysis. Risky drinking was defined as drinking > 2 drinks per day. The participants were divided into no risky drinking, past risky drinking, and current risky drinking groups. The prevalence of risky drinking, daily dosage, and type of alcohol beverages were assessed. The correlates of risky drinking were analyzed using logistic regression.

Results: The prevalence of past and current risky drinking was 6.2 and 4.4%, respectively. A total of 12.2% of males and 2.1% of females reported past risky drinking, and 8.9% of males and 1.4% of females reported current risky drinking. The median of the daily dosage of the past risky drinking group was 4.5 and 4 drinks in males and females, respectively, and were 4 and 3.3, respectively, of the current risky drinking group. Strong liquor was the most popular alcohol beverage in all groups. Men who were older or had white-collar work were less likely to be past risky drinkers, while those with smoking in past or current or heart disease were more likely to be past risky drinkers. Women who smoked in the past were more likely to be past risky drinkers. Men with older age or living in the urban areas or with heart disease were less likely to be current risky drinkers. Women with higher education or with heart disease were less likely to be current risky drinkers. Women with current smoking were more likely to have current risky drinking.

Conclusions: Our findings indicated that risky drinking among the oldest-old was not rare in China. The correlates of past and current risky drinking were different. Men and women had various correlates of risky drinking as well. Those with higher socioeconomic status seemed less likely to be risky drinking. More attention should be given to risky drinking among the oldest old, and sex-specific intervention may be needed.
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http://dx.doi.org/10.3389/fpsyt.2022.919888DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9195865PMC
May 2022

Cardiovascular Magnetic Resonance in Early Detection of Radiation Associated Cardiotoxicity With Chest Radiation.

Front Cardiovasc Med 2022 31;9:867479. Epub 2022 May 31.

Cardiovascular Division, Department of Medicine, Perelman School of Medicine of the University of Pennsylvania, Philadelphia, PA, United States.

Background: Chest radiation therapy (RT) is known to be associated with cardiotoxicity. However, the changes in myocardial tissue characterization with radiation-induced cardiotoxicity are not well-understood.

Objectives: This study sought to assess the changes in left ventricular function and tissue characterization using cardiovascular magnetic resonance (CMR) in patients receiving RT.

Materials And Methods: Between June 2015 and July 2018, we enrolled patients with breast, lung cancer, or lymphoma with plan to receive chest radiation after chemotherapy. CMR was performed using a 1.5T scanner at baseline and 6 months after RT. Myocardial volume, function, strain analysis using feature tracking, and tissue characterization including late gadolinium enhancement (LGE), T1, T2, T1ρ (rho), and extracellular volume fraction (ECV) were measured and compared using non-parametric methods.

Results: The final cohort consisted of 16 patients, 11 of whom completed both baseline and follow-up CMRs. Patients were matched to 10 healthy controls. At baseline prior to RT, compared to controls, patients had lower global circumferential strain (GCS) (15.3 ± 2.2% vs.18.4 ± 2.1%, = 0.004), and elevated T2 (47.9 ± 4.8 ms vs. 45.0 ± 1.5 ms, = 0.04) and T1ρ values (78.4 ± 5.9 vs. 66.9 ± 4.6 ms, < 0.001). Two patients had LGE. There was no significant difference in the average T1 values or ECV. There was a trend toward lower LV ejection fraction and global longitudinal strain (GLS). At 6-month follow-up after RT, there were no significant changes in all the CMR parameters.

Conclusion: At 6-month following chest radiation therapy, there was no change in LV and RV EF, LV and RV GLS, LV GCS, and myocardial tissue characterization using LGE, T1, ECV, T2, and T1ρ in a small cohort of patients. However, the baseline T2 and T1ρ were elevated and LV GCS was reduced compared to controls indicating ongoing myocardial edema and subclinical dysfunction post-chemotherapy.
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http://dx.doi.org/10.3389/fcvm.2022.867479DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9192956PMC
May 2022

Cavitation assisted endoplasmic reticulum targeted sonodynamic droplets to enhanced anti-PD-L1 immunotherapy in pancreatic cancer.

J Nanobiotechnology 2022 Jun 16;20(1):283. Epub 2022 Jun 16.

Department of Ultrasound in Medicine, The Second Affiliated Hospital of Zhejiang University School of Medicine, Zhejiang University, Hangzhou, 310000, China.

Background: Sonodynamic therapy (SDT) induces immunogenic cell death (ICD) in tumors and promises to play an assistive role in immunotherapy in pancreatic cancer. However, the short half-life and limited diffusion distance of reactive oxygen species (ROS) impair ICD induction, especially in tumors with relatively poor blood perfusion and dense stroma.

Results: To address this problem, we fabricated cavitation-assisted endoplasmic reticulum (ER) targeted sonodynamic nanodroplets (PMPS NDs, 329 nm). The good sonodynamic effect and precise endoplasmic reticulum target effect was verified. After intravenous injection, the cRGD peptide modified nanodroplets initially aggregated around the tumor vascular endothelial cells. Stimulated by ultrasound, the liquid-to-gas bubbles began to oscillate and cavitate. This acoustic droplet evaporation strategy facilitated transport of the nanoparticle across the vessel, with deep penetration. This loosened the tumor stroma and facilitated accumulation and penetration of loaded sonosensitizer after 6 h. The modified sonosensitizer can selectively accumulate in the ER to generate a large amount of ROS in situ, inducing potent ER stress, amplified ICD and dendritic cell maturation in vitro and in vivo. Furthermore, the elevated antitumor effect of SDT plus anti-PD-L1 immunotherapy was verified using an orthotopic tumor model.

Conclusions: This study reports a cavitation assisted ER targeted sonodynamic therapy that can enhance the effect of anti-PD-L1 immunotherapy effectively in orthotopic and distant pancreatic cancer.
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http://dx.doi.org/10.1186/s12951-022-01459-wDOI Listing
June 2022
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