Publications by authors named "Ying Yu"

1,109 Publications

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LA14 Alleviates Liver Injury.

mSystems 2021 Jun 15:e0038421. Epub 2021 Jun 15.

Key Laboratory of Nutrition of Zhejiang Province, Institute of Health Food, Hangzhou Medical College, Hangzhou, China.

Although the probiotic Lactobacillus acidophilus LA14 is used worldwide, its effect on liver diseases remains unelucidated. Here, 32 rats were divided into four groups, gavaged with L. acidophilus LA14 (3 × 10 CFU) or phosphate-buffered saline for 7 days, and then intraperitoneally injected with d-galactosamine or saline. After 24 h, blood, liver, ileum, and feces samples were collected for liver injury, inflammation, intestinal barrier, gut microbiota, metabolome, and transcriptome analyses. Pretreatment with L. acidophilus LA14 alleviated the d-galactosamine-induced elevation of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and bile acids; mitigated the histological injury to the liver and gut; and suppressed the inflammatory cytokines macrophage inflammatory protein 1α (MIP-1α), MIP-3α, and MCP-1. L. acidophilus LA14 also ameliorated the d-galactosamine-induced dysbiosis of the gut microbiota and metabolism, such as the enrichment of sp. strain dnLKV3 and the depletion of Streptococcus, butanoic acid, and acetyl-d-glucosamine. The underlying mechanism of L. acidophilus LA14 included prevention of not only the d-galactosamine-induced upregulation of infection- and tumor-related pathways but also the d-galactosamine-induced downregulation of antioxidation-related pathways during this process, as reflected by the liver transcriptome and proteome analyses. Furthermore, the administration of L. acidophilus LA14 to healthy rats did not alter the tested liver indicators but significantly enriched the beneficial and species, promoted metabolism and regulated pathways to improve immunity. The ability of L. acidophilus LA14 to alleviate liver injury was further confirmed with an acetaminophen-induced mouse model. These results might provide a reference for future studies on the application of L. acidophilus LA14 for the prevention of liver injury. The probiotic Lactobacillus acidophilus LA14 is widely used, but its effect on liver diseases has not been elucidated. We explored the protective effect of L. acidophilus LA14 on the liver using rats with d-galactosamine-induced liver injury. Pretreatment with L. acidophilus LA14 alleviated the d-galactosamine-induced elevation of serum ALT, AST, ALP, and bile acids, mitigated the histological injury to the liver and gut, and suppressed the inflammatory cytokines MIP-1α, MIP-3α, and MCP-1. These effects were correlated with the modulations of the gut microbiome, metabolome, and hepatic gene expression induced by L. acidophilus LA14. Moreover, the ability of L. acidophilus LA14 to alleviate liver injury was further confirmed with an acetaminophen-induced mouse model. These results might provide a reference for future studies on the application of L. acidophilus LA14 for the prevention of liver injury.
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http://dx.doi.org/10.1128/mSystems.00384-21DOI Listing
June 2021

Failures of stereoscopic shape constancy over changes of viewing distance and size for bilaterally symmetric polyhedra.

J Vis 2021 Jun;21(6)

Department of Psychology, The Ohio State University, Columbus, OH, USA.

Two shape matching experiments examined the effects of viewing distance and object size on observers' judgments of 3D metric shape under binocular viewing. Unlike previous studies on this topic, the stimuli were specifically designed to satisfy the minimal conditions for computing veridical shape from symmetry. Concretely, the stimuli were complex, mirror-symmetric polyhedra whose symmetry planes were oriented at an angle of 45o relative to the line of sight in a shape-matching task. Although it is mathematically possible to accurately compute the 3D shapes of these stimuli using relatively simple algorithms, the results indicated that human observers are unable to do so. Indeed, the apparent shapes of the objects were systematically expanded or compressed in depth as a function of viewing distance, in exactly the same way as has been reported for simpler stimuli that do not satisfy the minimal conditions for an accurate computational analysis. For objects presented at near distances, we also obtained statistically significant effects of object size on observers' shape judgments.
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http://dx.doi.org/10.1167/jov.21.6.5DOI Listing
June 2021

Coagulation factors and the incidence of COVID-19 severity: Mendelian randomization analyses and supporting evidence.

Signal Transduct Target Ther 2021 06 7;6(1):222. Epub 2021 Jun 7.

The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, Tianjin Medical University, Tianjin, China.

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http://dx.doi.org/10.1038/s41392-021-00640-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8181534PMC
June 2021

Identification of new building blocks by fragment screening for discovering GyrB inhibitors.

Bioorg Chem 2021 May 31;114:105040. Epub 2021 May 31.

Research Center for Drug Discovery and Guangdong Provincial Key Laboratory of Chiral Molecule and Drug Discovery, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China. Electronic address:

DNA gyrase is an essential DNA topoisomerase that exists only in bacteria. Since novobiocin was withdrawn from the market, new scaffolds and new mechanistic GyrB inhibitors are urgently needed. In this study, we employed fragment screening and X-ray crystallography to identify new building blocks, as well as their binding mechanisms, to support the discovery of new GyrB inhibitors. In total, 84 of the 618 chemical fragments were shown to either thermally stabilize the ATPase domain of Escherichia coli GyrB or inhibit the ATPase activity of E. coli gyrase. Among them, the IC values of fragments 10 and 23 were determined to be 605.3 μM and 446.2 μM, respectively. Cocrystal structures of the GyrB ATPase domain with twelve fragment hits were successfully determined at a high resolution. All twelve fragments were deeply inserted in the pocket and formed H-bonds with Asp73 and Thr165, and six fragments formed an additional H-bond with the backbone oxygen of Val71. Fragment screening further highlighted the capability of Asp73, Thr165 and Val71 to bind chemicals and provided diverse building blocks for the design of GyrB inhibitors.
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http://dx.doi.org/10.1016/j.bioorg.2021.105040DOI Listing
May 2021

Construction of DNA Tools for Hyperexpression in Chloroplasts.

ACS Synth Biol 2021 Jun 7. Epub 2021 Jun 7.

Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, U.K.

Chloroplasts are attractive platforms for synthetic biology applications since they are capable of driving very high levels of transgene expression, if mRNA production and stability are properly regulated. However, plastid transformation is a slow process and currently limited to a few plant species. The liverwort is a simple model plant that allows rapid transformation studies; however, its potential for protein hyperexpression has not been fully exploited. This is partially due to the fact that chloroplast post-transcriptional regulation is poorly characterized in this plant. We have mapped patterns of transcription in chloroplasts. Furthermore, we have obtained and compared sequences from 51 bryophyte species and identified putative sites for pentatricopeptide repeat protein binding that are thought to play important roles in mRNA stabilization. Candidate binding sites were tested for their ability to confer high levels of reporter gene expression in chloroplasts, and levels of protein production and effects on growth were measured in homoplastic transformed plants. We have produced novel DNA tools for protein hyperexpression in this facile plant system that is a test-bed for chloroplast engineering.
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http://dx.doi.org/10.1021/acssynbio.0c00637DOI Listing
June 2021

A New Hydrogen Sensor Fault Diagnosis Method Based on Transfer Learning With LeNet-5.

Front Neurorobot 2021 21;15:664135. Epub 2021 May 21.

Key Laboratory of Electronics Engineering, College of Heilongjiang Province, Heilongjiang University, Harbin, China.

The fault safety monitoring of hydrogen sensors is very important for their practical application. The precondition of traditional machine learning methods for sensor fault diagnosis is that enough fault data with the same distribution and feature space under the same working environment must exist. Widely used fault diagnosis methods are not suitable for real working environments because they are easily complicated by environmental conditions such as temperature, humidity, shock, and vibration. Under the influence of such complex conditions, the acquisition of sensor fault data is limited. In order to improve fault diagnosis accuracy under complex environmental conditions, a novel method of transfer learning (TL) with LeNet-5 is proposed in this paper. Firstly, LeNet-5 is applied to learn the features of the data-rich datasets of gas sensor faults in a normal environment and to adjust the parameters accordingly. The parameters of the LeNet-5 are transferred from the task in the normal environment to a task in a complex environment by using the TL method. Then, the migrated LeNet-5 is used for the fault diagnosis of gas sensors with a small amount of fault data in a complex environment. Finally, a prototype hydrogen sensor array is designed and implemented for experimental verification. The gas sensor fault diagnosis accuracy of the traditional LeNet-5 was 88.48 ± 1.04%, while the fault diagnosis accuracy of TL with LeNet-5 was 92.49 ± 1.28%. The experimental results show that the method adopted presents an excellent solution for the fault diagnosis of a hydrogen sensor using a small quantity of fault data obtained under complex environmental conditions.
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http://dx.doi.org/10.3389/fnbot.2021.664135DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175791PMC
May 2021

A seven-gene prognostic signature predicts overall survival of patients with lung adenocarcinoma (LUAD).

Cancer Cell Int 2021 Jun 6;21(1):294. Epub 2021 Jun 6.

Center of Genome and Personalized Medicine, Institute of Cancer Stem Cell, Dalian Medical University, Dalian, 116044, Liaoning, People's Republic of China.

Background: Lung adenocarcinoma (LUAD) is one of the most common types in the world with a high mortality rate. Despite advances in treatment strategies, the overall survival (OS) remains short. Our study aims to establish a reliable prognostic signature closely related to the survival of LUAD patients that can better predict prognosis and possibly help with individual monitoring of LUAD patients.

Methods: Raw RNA-sequencing data were obtained from Fudan University and used as a training group. Differentially expressed genes (DEGs) for the training group were screened. The univariate, least absolute shrinkage and selection operator (LASSO), and multivariate cox regression analysis were conducted to identify the candidate prognostic genes and construct the risk score model. Kaplan-Meier analysis, time-dependent receiver operating characteristic (ROC) curve were used to evaluate the prognostic power and performance of the signature. Moreover, The Cancer Genome Atlas (TCGA-LUAD) dataset was further used to validate the predictive ability of prognostic signature.

Results: A prognostic signature consisting of seven prognostic-related genes was constructed using the training group. The 7-gene prognostic signature significantly grouped patients in high and low-risk groups in terms of overall survival in the training cohort [hazard ratio, HR = 8.94, 95% confidence interval (95% CI)] [2.041-39.2]; P = 0.0004), and in the validation cohort (HR = 2.41, 95% CI [1.779-3.276]; P < 0.0001). Cox regression analysis (univariate and multivariate) demonstrated that the seven-gene signature is an independent prognostic biomarker for predicting the survival of LUAD patients. ROC curves revealed that the 7-gene prognostic signature achieved a good performance in training and validation groups (AUC = 0.91, AUC = 0.7 respectively) in predicting OS for LUAD patients. Furthermore, the stratified analysis of the signature showed another classification to predict the prognosis.

Conclusion: Our study suggested a new and reliable prognostic signature that has a significant implication in predicting overall survival for LUAD patients and may help with early diagnosis and making effective clinical decisions regarding potential individual treatment.
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http://dx.doi.org/10.1186/s12935-021-01975-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183047PMC
June 2021

Comprehensive analysis of the lysine acetylome and succinylome in the hippocampus of gut microbiota-dysbiosis mice.

J Adv Res 2021 05 7;30:27-38. Epub 2020 Dec 7.

Department of Neurology, Yongchuan Hospital of Chongqing Medical University, Chongqing 402160, China.

Introduction: Major depressive disorder is caused by gene-environment interactions, and the host microbiome has been recognized as an important environmental factor. However, the underlying mechanisms of the host-microbiota interactions that lead to depression are complex and remain poorly understood.

Objectives: The present study aimed to explore the possible mechanisms underlying gut microbiota dysbiosis-induced depressive-like behaviors.

Methods: We used high-performance liquid chromatography-tandem mass spectrometry to analyze alterations in the hippocampal lysine acetylome and succinylome in male mice that had received gut microbiota from fecal samples of either patients with major depressive disorder or healthy controls. This was followed by bioinformatic analyses.

Results: A total of 315 acetylation sites on 223 proteins and 624 succinylation sites on 494 proteins were differentially expressed in the gut microbiota-dysbiosis mice. The significantly acetylated proteins were primarily associated with carbon metabolism disruption and gene transcription suppression, while the synaptic vesicle cycle and protein translation were the most significantly altered functions for succinylated proteins. Additionally, our findings suggest that gut microbiota dysbiosis disturbs mitochondria-mediated biological processes and the MAPK signaling pathway through crosstalk between acetylation and succinylation on relevant proteins.

Conclusions: This is the first study to demonstrate modifications in acetylation and succinylation in gut microbiota-dysbiosis mice. Our findings provide new avenues for exploring the pathogenesis of gut microbiota dysbiosis-related depression, and highlight potential targets for depression treatment.
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http://dx.doi.org/10.1016/j.jare.2020.12.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8132208PMC
May 2021

Genome-wide identification and expression analysis of the WRKY transcription factor family in flax (Linum usitatissimum L.).

BMC Genomics 2021 May 22;22(1):375. Epub 2021 May 22.

Heilongjiang Academy of Agricultural Sciences, Harbin, 150086, China.

Background: Members of the WRKY protein family, one of the largest transcription factor families in plants, are involved in plant growth and development, signal transduction, senescence, and stress resistance. However, little information is available about WRKY transcription factors in flax (Linum usitatissimum L.).

Results: In this study, comprehensive genome-wide characterization of the flax WRKY gene family was conducted that led to prediction of 102 LuWRKY genes. Based on bioinformatics-based predictions of structural and phylogenetic features of encoded LuWRKY proteins, 95 LuWRKYs were classified into three main groups (Group I, II, and III); Group II LuWRKYs were further assigned to five subgroups (IIa-e), while seven unique LuWRKYs (LuWRKYs 96-102) could not be assigned to any group. Most LuWRKY proteins within a given subgroup shared similar motif compositions, while a high degree of motif composition variability was apparent between subgroups. Using RNA-seq data, expression patterns of the 102 predicted LuWRKY genes were also investigated. Expression profiling data demonstrated that most genes associated with cellulose, hemicellulose, or lignin content were predominantly expressed in stems, roots, and less in leaves. However, most genes associated with stress responses were predominantly expressed in leaves and exhibited distinctly higher expression levels in developmental stages 1 and 8 than during other stages.

Conclusions: Ultimately, the present study provides a comprehensive analysis of predicted flax WRKY family genes to guide future investigations to reveal functions of LuWRKY proteins during plant growth, development, and stress responses.
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http://dx.doi.org/10.1186/s12864-021-07697-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8141250PMC
May 2021

Clinical significance of MDRO screening and infection risk factor analysis in the ICU.

Am J Transl Res 2021 15;13(4):3717-3723. Epub 2021 Apr 15.

Department of Laboratory Medicine, West China Second University Hospital, Sichuan University Chengdu 610041, Sichuan Province, China.

Objective: This study aimed to investigate the clinical significance of multi-drug resistant organism (MDRO) screening and infection risk factor analysis in the intensive care unit (ICU).

Method: A total of 210 patients treated in the ICU of our hospital were enrolled as the study subjects, and were divided into the MDRO group (n=100 cases) and the non-MDRO group (n=110) according to the presence or absence of MDRO infection after examination of the pharyngeal swabs. The pathogens of MDRO infection and drug resistance were analyzed. The single-factor as well as multifactor logistic regression analysis of MDRO infections were carried out and the 30-d mortality rate, hospitalization time and treatment costs were compared between the two groups.

Results: A total of 158 MDRO strains were detected in 100 patients with MDRO infection, of which G-84 accounted for 53.16% and G+ 74 accounted for 46.84%. The resistance analysis revealed that G-MDRO was sensitive to imipenem and G+ MDRO was sensitive to vancomycin, and no vancomycin-resistant MDROs were found. The logistic regression model and multifactorial analysis showed that mechanical ventilation, arterial and venous intubation, implementation of fiberoptic bronchoscopy, concurrent chronic lung disease and chronic cardiovascular disease were independent risk factors for the development of MDRO infection (<0.05). The length of hospital stay, cost of treatment, and 30-d mortality rate in the MDRO group were significantly higher than those in the non-MDRO group (<0.05).

Conclusion: ICU mechanical ventilation, arterial and intravenous intubation, fiberoptic bronchoscopy, concurrent chronic lung disease and chronic cardiovascular disease are the independent risk factors for MDRO infection.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129317PMC
April 2021

A feedback loop: Interactions between Inflammatory Signals and Clonal Hematopoiesis in Cardiovascular Disease.

Mol Biol Rep 2021 Apr 13;48(4):3785-3798. Epub 2021 May 13.

First Affiliated Hospital of Harbin Medical University, Harbin, 150001, China.

Age and inflammation are powerful drivers of cardiovascular disease. With the growing recognition that traditional cardiovascular risk factors are not fully accurate predictors of cardiovascular disease, recent studies have revealed the prevalence of positive selection of somatic cell mutations in hematopoietic stem cells in the elderly population, which can cause clonal hematopoiesis. Interestingly, clonal hematopoiesis is not only associated with cancer and death, but also closely related to the risk of increased cardiovascular disease due to mutations in TET2, DNMT3A, ASXL1, and JAK2. However, the mechanism of the interaction of clonal hematopoiesis and cardiovascular disease is only partially understood. In mice, somatic mutations have led to significantly increased expression of inflammatory genes in innate immune cells, which may explain the relationship between mutations and cardiovascular disease. Here, we further discuss the association between inflammatory signaling, clonal hematopoiesis, and cardiovascular disease,and using two hypotheses to propose a feedback loop between inflammatory signaling and clonal hematopoiesis for getting insight into the pathogenesis of cardiovascular diseases in depth. Therapies targeting mutant clones or increased inflammatory mediators may be useful for ameliorating the risk of cardiovascular disease.
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http://dx.doi.org/10.1007/s11033-021-06370-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8117808PMC
April 2021

DIA proteomics analysis through serum profiles reveals the significant proteins as candidate biomarkers in women with PCOS.

BMC Med Genomics 2021 May 9;14(1):125. Epub 2021 May 9.

Institute of Laboratory Medicine, Jiangsu Key Laboratory of Laboratory Medicine, Jiangsu University, No. 301 Xuefu Road, Zhenjiang, Jiangsu, 210013, People's Republic of China.

Background: The aim of this study was to apply proteomic methodology for the analysis of proteome changes in women with polycystic ovary syndrome (PCOS).

Material And Methods: All the participators including 31 PCOS patients and 31 healthy female as controls were recruited, the clinical characteristics data was recorded at the time of recruitment, the laboratory biochemical data was detected. Then, a data-independent acquisition (DIA)-based proteomics method was performed to compare the serum protein changes between PCOS patients and controls. In addition, Western blotting was used to validate the expression of identified proteomic biomarkers.

Results: There were 80 proteins differentially expressed between PCOS patients and controls significantly, including 54 downregulated and 26 upregulated proteins. Gene ontology and Kyoto Encyclopedia of Genes and Genomes analysis showed that downregulated proteins were enriched in platelet degranulation, cell adhesion, cell activation, blood coagulation, hemostasis, defense response and inflammatory response terms; upregulated proteins were enriched in cofactor catabolic process, hydrogen peroxide catabolic process, antioxidant activity, cellular oxidant detoxification, cellular detoxification, antibiotic catabolic process and hydrogen peroxide metabolic process. Receiver operating characteristic curves analysis showed that the area under curve of Histone H4 (H4), Histone H2A (H2A), Trem-like transcript 1 protein (TLT-1) were all over than 0.9, indicated promising diagnosis values of these proteins. Western blotting results proved that the detected significant proteins, including H4, H2A, TLT-1, Peroxiredoxin-1, Band 3 anion transport protein were all differently expressed in PCOS and control groups significantly.

Conclusion: These proteomic biomarkers provided the potentiality to help us understand PCOS better, but future studies comparing systemic expression and exact role of these candidate biomarkers in PCOS are essential for confirmation of this hypothesis.
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http://dx.doi.org/10.1186/s12920-021-00962-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106864PMC
May 2021

Field study on the transportation characteristics of PFASs from water source to tap water.

Water Res 2021 Jun 18;198:117162. Epub 2021 Apr 18.

Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Perfluoroalkyl substances (PFASs) can occur in water sources, pass through drinking water treatment plants (DWTPs), drinking water distribution systems (DWDSs), to the consumer taps. This investigation was carried out to present the transportation behaviors of 17 PFASs, involving seven DWTPs with different water sources, raw water transportation modes, treatment processes, and DWDS structures in eastern and northern China. The results showed that the long-distance raw water transportation pipelines removed a certain extent of PFASs from raw water, probably due to the accumulation of loose deposits. The long-distance, open-channel South-to-North water diversion increased PFAS contamination risk. In the DWTPs, granular activated carbon (GAC) adsorption and ultraviolet radiation removed less than 25% of PFASs, but ozonation-biological activated carbon (O-BAC) was superior to GAC alone in removing PFASs. Loose deposits couldsignificantly influence PFAS accumulation and release within branch-structured DWDSs. In loop-structured DWDSs, finished water with different PFAS characteristics could mix along the pipeline, with the corresponding DWTP as the center, ultimately forming a relatively uniform distribution in the entire DWDS.
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http://dx.doi.org/10.1016/j.watres.2021.117162DOI Listing
June 2021

Obesity, Even in the Metabolically Healthy, Increases the Risk of Poor Physical Performance: A Cross-Sectional Study of Older People in a Chinese Community.

Clin Interv Aging 2021 27;16:697-706. Epub 2021 Apr 27.

Department of Rehabilitation Medicine, Shanghai University of Medicine and Health Sciences, Shanghai, People's Republic of China.

Objective: We examined the association between obesity and physical performance under different metabolic status.

Methods: The sample included 1395 Chinese community-dwelling participants (mean age, 71.88 ± 5.87 years; 40.9% men). Being metabolically healthy was defined as having the presence of < 3 of 5 components of metabolic syndrome (MetS); obesity was defined as having a BMI > 28 kg/m. Participants were divided into four groups based on BMI (non-obese/obese) and metabolic health (healthy/unhealthy). Physical performance was measured by grip strength, 4-m walking speed, and the timed up and go test (TUGT).

Results: After multiple adjustments, compared with metabolically healthy non-obese group, the metabolically unhealthy obese group showed lower relative grip strength, lower 4-m walking speed, and higher TUGT ( all < 0.05), and only relative grip strength of the metabolically healthy obese group was significantly lower than that of metabolically healthy non-obese ( < 0.01). Relative grip strength was negatively associated with impaired fasting glucose (β = -0.071), elevated triglycerides (β = -0.062), abdominal obesity (β = -0.230) and general obesity (β = -0.225) ( all < 0.01). Walking speed and TUGT were only associated with general obesity, rather than other metabolic components. The associations of MetS with physical performance were mainly driven by abdominal obesity.

Conclusion: Even in those who are metabolically healthy, obesity (especially general obesity) increases the risk of poor physical performance. Elderly people with general obesity and MetS, whether in combination or alone, have an increased risk of muscle dysfunction, and that combination produces a higher risk of impaired mobility.
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http://dx.doi.org/10.2147/CIA.S302167DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8088299PMC
June 2021

Sevoflurane Postconditioning Attenuates Hepatic Ischemia-Reperfusion Injury by Limiting HMGB1/TLR4/NF-κB Pathway Modulating microRNA-142 .

Front Pharmacol 2021 16;12:646307. Epub 2021 Apr 16.

Department of Anesthesiology, Zhongshan Hospital Fudan University, Shanghai, China.

Preconditioning of sevoflurane (Sevo) has been demonstrated to protect the liver from ischemia/reperfusion (I/R) injury. However, it is unknown whether it has hepatoprotective when given at the onset of reperfusion (postconditioning), a protocol with more clinical impact. The present study aimed to explore the hepatoprotective effects of Sevo postconditioning against hepatic IR injury and and the possible mechanisms. Using a mouse model of hepatic I/R, Sevo postconditioning significantly improved hepatic injury after reperfusion, as demonstrated by reduced AST, ALT, and LDH serum levels and reduced histologic damage in liver tissues. Furthermore, Sevo postconditioning could suppress the apoptosis, inhibit oxidative stress and inflammatory response in liver tissue of HIRI mice, as well as improve the survival rate of HIRI mice. Through analyzing GSE72314 from the gene expression omnibus (GEO) database, it was demonstrated that microRNA (miR)-142 is downregulated by HIRI, which was reversed by Sevo treatment. Further investigation showed that agomiR-142 injection could enhance the hepatoprotective effects of Sevo postconditioning on I/R injury, while antagomiR-142 reversed these effects in mice. Notably, high mobility group box 1 (HMGB1), an important inflammatory factor, was directly targeted by miR-142 in hepatic cells, and we further found that Sevo could inhibit the expression of HMGB1 through up-regulating miR-142 expression in HIRI mice model. In addition, we found that I/R injury induced the activation of TLR4/NF-κB inflammatory pathway was partially suppressed by Sevo postconditioning, and miR-142 mediated the regulatory role of Sevo postconditioning. In line with the results, Sevo treatment improved the cell viability, inhibited cell apoptosis, oxidative stress and inflammatory response HIRI model, while these effects were reversed by antagomiR-142 transfection. Collectively, our findings demonstrated that Sevo postconditioning counteracts the downregulation of miR-142 provoked by I/R, in turn decreased the expression of HMGB1, blocking TLR4/NF-κB pathway activation, thus improving hepatic I/R injury. Our data suggest that Sevo may be a valuable alternative anaesthetic agent in liver transplantation and major liver surgeries.
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http://dx.doi.org/10.3389/fphar.2021.646307DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085516PMC
April 2021

REGγ regulates hair cycle by activating Lgr5 positive hair follicle stem cells.

J Dermatol Sci 2021 May 18;102(2):101-108. Epub 2021 Apr 18.

Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, School of Life Sciences, East China Normal University, Shanghai, China. Electronic address:

Background: REGγ acts as a proteasome activating factor mediating proteasome degradation of substrate proteins in an ATP and ubiquitination independent manner and also as an important regulator of cell cycle, proliferation and apoptosis. Hair cycle involves dynamic, continuous morphological changes of three stages (anagen, catagen and telogen).

Objective: The function of REGγ in hair cycling is still unclear.

Methods: Here, we used REGγ knockout 293 T cells, inducible 293WT and 293N151Y cell, REGγ knockout mice to identify the novel molecular mechanism of REGγ in regulating hair follicle stem cells.

Results: In the present study, we found that REGγ deletion markedly delayed the transition of hair follicles from telogen to anagen and hair regeneration in mice. We also observed significant decrease of hair follicle stem cell number, stem-like property and proliferation ability. Interestingly, the results from real-time PCR, FACS, Western Blot and immunofluorescent analysis showed that REGγ deletion could greatly downregulate Lgr5 expression in the hair follicles. Meanwhile, REGγ was demonstrated to directly interact with LHX2 and promotes its degradation. Importantly, REGγ specific deletion in Lgr5 stem cells induced the marked delay of hair regeneration after depilation.

Conclusion: These data together indicate that REGγ was a new mediator of Lgr5 expression in hair follicle at least partly by promoting the degradation of its suppressive transcription factor LHX2. It seemed that REGγ regulated hair anagen entry and hair regrowth by activating Lgr5 positive hair follicle stem cells.
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http://dx.doi.org/10.1016/j.jdermsci.2021.04.002DOI Listing
May 2021

Functional consequences of a rare missense BARD1 c.403G>A germline mutation identified in a triple-negative breast cancer patient.

Breast Cancer Res 2021 May 1;23(1):53. Epub 2021 May 1.

State Key Laboratory of Genetic Engineering, School of Life Sciences and Shanghai Cancer Center, Fudan University, Shanghai, China.

We identified a rare missense germline mutation in BARD1 (c.403G>A or p.Asp135Asn) as pathogenic using integrated genomics and transcriptomics profiling of germline and tumor samples from an early-onset triple-negative breast cancer patient who later was administrated with a PARP inhibitor for 2 months. We demonstrated in cell and mouse models that, compared to the wild-type, (1) c.403G>A mutant cell lines were more sensitive to irradiation, a DNA damage agent, and a PARP inhibitor; (2) c.403G>A mutation inhibited interaction between BARD1 and RAD51 (but not BRCA1); and (3) c.403G>A mutant mice were hypersensitive to ionizing radiation. Our study shed lights on the clinical interpretation of rare germline mutations of BARD1.
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http://dx.doi.org/10.1186/s13058-021-01428-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8088670PMC
May 2021

Classification of Prostate Transitional Zone Cancer and Hyperplasia Using Deep Transfer Learning From Disease-Related Images.

Cureus 2021 Mar 25;13(3):e14108. Epub 2021 Mar 25.

Department of Radiology, Fourth Military Medical University, Shaanxi, CHN.

Purpose The diagnosis of prostate transition zone cancer (PTZC) remains a clinical challenge due to their similarity to benign prostatic hyperplasia (BPH) on MRI. The Deep Convolutional Neural Networks (DCNNs) showed high efficacy in diagnosing PTZC on medical imaging but was limited by the small data size. A transfer learning (TL) method was combined with deep learning to overcome this challenge. Materials and methods A retrospective investigation was conducted on 217 patients enrolled from our hospital database (208 patients) and The Cancer Imaging Archive (nine patients). Using T2-weighted images (T2WIs) and apparent diffusion coefficient (ADC) maps, DCNN models were trained and compared between different TL databases (ImageNet vs. disease-related images) and protocols (from scratch, fine-tuning, or transductive transferring). Results PTZC and BPH can be classified through traditional DCNN. The efficacy of TL from natural images was limited but improved by transferring knowledge from the disease-related images. Furthermore, transductive TL from disease-related images had comparable efficacy to the fine-tuning method. Limitations include retrospective design and a relatively small sample size. Conclusion Deep TL from disease-related images is a powerful tool for an automated PTZC diagnostic system. In developing regions where only conventional MR scans are available, the accurate diagnosis of PTZC can be achieved via transductive deep TL from disease-related images.
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http://dx.doi.org/10.7759/cureus.14108DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8075764PMC
March 2021

Wafer-Scale Epitaxial Low Density InAs/GaAs Quantum Dot for Single Photon Emitter in Three-Inch Substrate.

Nanomaterials (Basel) 2021 Apr 6;11(4). Epub 2021 Apr 6.

State Key Laboratory of Optoelectronic Materials and Technologies, School of Electronics and Information Technology, Sun Yat-sen University, Guangzhou 510275, China.

In this work, we successfully achieved wafer-scale low density InAs/GaAs quantum dots (QDs) for single photon emitter on three-inch wafer by precisely controlling the growth parameters. The highly uniform InAs/GaAs QDs show low density of μ0.96/μm2 within the radius of 2 cm. When embedding into a circular Bragg grating cavity on highly efficient broadband reflector (CBR-HBR), the single QDs show excellent optoelectronic properties with the linewidth of 3± 0.08 GHz, the second-order correlation factor g2(τ)=0.0322 ±0.0023, and an exciton life time of 323 ps under two-photon resonant excitation.
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http://dx.doi.org/10.3390/nano11040930DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8067461PMC
April 2021

Gastric polyp detection in gastroscopic images using deep neural network.

PLoS One 2021 28;16(4):e0250632. Epub 2021 Apr 28.

School of Automation, Southeast University, Nanjing, China.

This paper presents the research results of detecting gastric polyps with deep learning object detection method in gastroscopic images. Gastric polyps have various sizes. The difficulty of polyp detection is that small polyps are difficult to detect from the background. We propose a feature extraction and fusion module and combine it with the YOLOv3 network to form our network. This method performs better than other methods in the detection of small polyps because it can fuse the semantic information of high-level feature maps with low-level feature maps to help small polyps detection. In this work, we use a dataset of gastric polyps created by ourselves, containing 1433 training images and 508 validation images. We train and validate our network on our dataset. In comparison with other methods of polyps detection, our method has a significant improvement in precision, recall rate, F1, and F2 score. The precision, recall rate, F1 score, and F2 score of our method can achieve 91.6%, 86.2%, 88.8%, and 87.2%.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0250632PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081222PMC
April 2021

[Research progress on pyroptosis and cardiovascular diseases].

Sheng Li Xue Bao 2021 Apr;73(2):329-341

Department of Physiology, Bengbu Medical College, Bengbu 233000, China.

Pyroptosis is a form of programmed cell death which is closely related to the inflammatory response, mediated by Gasdermin protein and depends on the activity of cysteine aspartate specific protease (caspase). Pyroptosis is typically characterized by swelling and rupture of cell membrane, release of proinflammatory factors and cell contents from the plasma membrane to the extracellular environment, which aggravates inflammatory response. During the inflammatory response, NLRP3, caspase, Gasdermin D (GSDMD) and IL-1β play important roles in the occurrence and development of cardiovascular diseases. In this review, we focus on the role of pyroptosis in cardiovascular diseases including atherosclerosis, coronary heart disease, myocardial infarction, diabetic cardiomyopathy, pressure overload-induced ventricular remodeling and cardiac hypertrophy, myocarditis, arrhythmia and so on, and summarize the potential treatment targeting pyroptosis. It will provide the basis for prevention and treatment of clinical cardiovascular diseases.
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April 2021

Comprehensive RNA-Seq Profiling Reveals Temporal and Tissue-Specific Changes in Gene Expression in Sprague-Dawley Rats as Response to Heat Stress Challenges.

Front Genet 2021 9;12:651979. Epub 2021 Apr 9.

Key Laboratory of Animal Genetics, Breeding and Reproduction, MARA, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China.

Understanding heat stress physiology and identifying reliable biomarkers are paramount for developing effective management and mitigation strategies. However, little is known about the molecular mechanisms underlying thermal tolerance in animals. In an experimental model of Sprague-Dawley rats subjected to temperatures of 22 ± 1°C (control group; CT) and 42°C for 30 min (H30), 60 min (H60), and 120 min (H120), RNA-sequencing (RNA-Seq) assays were performed for blood (CT and H120), liver (CT, H30, H60, and H120), and adrenal glands (CT, H30, H60, and H120). A total of 53, 1,310, and 1,501 differentially expressed genes (DEGs) were significantly identified in the blood ( < 0.05 and |fold change (FC)| >2), liver ( < 0.01, false discovery rate (FDR)-adjusted = 0.05 and |FC| >2) and adrenal glands ( < 0.01, FDR-adjusted = 0.05 and |FC| >2), respectively. Of these, four DEGs, namely , and , were shared among the three tissues in CT vs. H120 comparison. Functional enrichment analyses of the DEGs identified in the blood (CT vs. H120) revealed 12 biological processes (BPs) and 25 metabolic pathways significantly enriched (FDR = 0.05). In the liver, 133 BPs and three metabolic pathways were significantly detected by comparing CT vs. H30, H60, and H120. Furthermore, 237 BPs were significantly (FDR = 0.05) enriched in the adrenal glands, and no shared metabolic pathways were detected among the different heat-stressed groups of rats. Five and four expression patterns ( < 0.05) were uncovered by 73 and 91 shared DEGs in the liver and adrenal glands, respectively, over the different comparisons. Among these, 69 and 73 genes, respectively, were proposed as candidates for regulating heat stress response in rats. Finally, together with genome-wide association study (GWAS) results in cattle and phenome-wide association studies (PheWAS) analysis in humans, five genes (, and ) were considered as being associated with heat stress response across mammal species. The datasets and findings of this study will contribute to a better understanding of heat stress response in mammals and to the development of effective approaches to mitigate heat stress response in livestock through breeding.
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http://dx.doi.org/10.3389/fgene.2021.651979DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063118PMC
April 2021

Autophagy blockage promotes the pyroptosis of ox-LDL-treated macrophages by modulating the p62/Nrf2/ARE axis.

J Physiol Biochem 2021 Apr 22. Epub 2021 Apr 22.

Department of Laboratory Diagnostics, the First Affiliated Hospital of Harbin Medical University, 23 Youzheng Street, NanGang, Harbin, Heilongjiang, 150001, People's Republic of China.

Atherosclerosis, a chronic comprehensive cardiovascular disease, is characterized by the lipid infiltration, formation of foam cells derived from macrophages and inflammation in the vessel wall. Substantial evidence confirms that the activity of autophagic bodies plays a pivot role in regulating cell deaths, but the mechanisms of autophagy to regulate the pyroptosis of macrophages in atherosclerosis remain unclear. In our study, we explored that ox-LDL decreased the cell viability and destroyed the integrity of cell membrane, resulting in the pyroptosis of THP-1 derived macrophages in a dose-dependent manner. Western blotting, qRT-PCR and ELISA also showed that chloroquine (CQ) could up-regulate the expression of p62 through impairing autophagy and induce the pyroptosis of macrophages treated by ox-LDL, as evidenced by the decrease of cell viability and membrane integrity, and the increase of pro-caspase-1, GSDMD, and proinflammatory factors IL-1β and IL-18. Further researches demonstrated that Nrf2, a nuclear factor activated by p62, was linked to macrophage pyroptosis. Overactivating or suppressing Nrf2/ARE signaling would correspondingly aggravate or alleviate pyroptosis, in which the level of p62 was regulated by Nrf2 feedback. Then, bioinformatic analysis verified that there was a close interaction between p62, Nrf2/ARE signaling proteins and pyroptosis-related proteins. Taken together, our results show that blocking autophagy promotes the pyroptosis of ox-LDL-treated macrophages via the p62/Nrf2/ARE axis, providing a novel therapeutic target for atherosclerosis.
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http://dx.doi.org/10.1007/s13105-021-00811-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8060691PMC
April 2021

Effectiveness and Safety of Romiplostim Among Patients with Newly Diagnosed, Persistent and Chronic Immune Thrombocytopenia in European Clinical Practice.

Adv Ther 2021 05 18;38(5):2673-2688. Epub 2021 Apr 18.

NoviSci, Inc., 201 W Main St, Ste 101, Durham, NC, 27701, USA.

Introduction: Romiplostim has been approved in Europe since 2009 to treat patients with chronic primary immune thrombocytopenia (ITP). Using real-world data from seven European countries, we measured the effectiveness and safety outcomes within 24 weeks following romiplostim initiation by duration of ITP: less than 3 months ("newly diagnosed"), 3-12 months ("persistent"), and more than 12 months ("chronic").

Methods: Adults with ITP and ≥ 1 romiplostim administration between 2009 and 2012 were included. Endpoints included durable platelet response, median platelet count, rescue therapy, bleeding and adverse events. We used inverse probability of censoring weighted estimators to estimate cumulative risk of each outcome. There were 64 newly diagnosed, 50 persistent, and 226 chronic ITP patients at romiplostim initiation.

Results: Durable platelet response at 24 weeks ranged from 32% [confidence interval (CI): 18-46%] in newly diagnosed patients to 53% (CI 37-68%) in persistent patients. Median platelet count during follow-up ranged from 88 (CI 80-96) × 10/L in chronic patients to 131 (CI 102-160) × 10/L in newly diagnosed patients.

Conclusion: Regardless of ITP duration, over half of patients discontinued concomitant ITP medications. Few adverse events were observed. Although only approved for chronic patients, estimates of the romiplostim treatment effect were similar across patients being managed in European clinical practice, regardless of ITP duration at romiplostim initiation.
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http://dx.doi.org/10.1007/s12325-021-01727-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107157PMC
May 2021

A verified genomic reference sample for assessing performance of cancer panels detecting small variants of low allele frequency.

Genome Biol 2021 Apr 16;22(1):111. Epub 2021 Apr 16.

Marketing, Integrated DNA Technologies, Inc., 1710 Commercial Park, Coralville, IA, 52241, USA.

Background: Oncopanel genomic testing, which identifies important somatic variants, is increasingly common in medical practice and especially in clinical trials. Currently, there is a paucity of reliable genomic reference samples having a suitably large number of pre-identified variants for properly assessing oncopanel assay analytical quality and performance. The FDA-led Sequencing and Quality Control Phase 2 (SEQC2) consortium analyze ten diverse cancer cell lines individually and their pool, termed Sample A, to develop a reference sample with suitably large numbers of coding positions with known (variant) positives and negatives for properly evaluating oncopanel analytical performance.

Results: In reference Sample A, we identify more than 40,000 variants down to 1% allele frequency with more than 25,000 variants having less than 20% allele frequency with 1653 variants in COSMIC-related genes. This is 5-100× more than existing commercially available samples. We also identify an unprecedented number of negative positions in coding regions, allowing statistical rigor in assessing limit-of-detection, sensitivity, and precision. Over 300 loci are randomly selected and independently verified via droplet digital PCR with 100% concordance. Agilent normal reference Sample B can be admixed with Sample A to create new samples with a similar number of known variants at much lower allele frequency than what exists in Sample A natively, including known variants having allele frequency of 0.02%, a range suitable for assessing liquid biopsy panels.

Conclusion: These new reference samples and their admixtures provide superior capability for performing oncopanel quality control, analytical accuracy, and validation for small to large oncopanels and liquid biopsy assays.
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http://dx.doi.org/10.1186/s13059-021-02316-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8051128PMC
April 2021

Cross-oncopanel study reveals high sensitivity and accuracy with overall analytical performance depending on genomic regions.

Genome Biol 2021 Apr 16;22(1):109. Epub 2021 Apr 16.

Greenwood Genetic Center, 106 Gregor Mendel Circle, Greenwood, SC, 29646, USA.

Background: Targeted sequencing using oncopanels requires comprehensive assessments of accuracy and detection sensitivity to ensure analytical validity. By employing reference materials characterized by the U.S. Food and Drug Administration-led SEquence Quality Control project phase2 (SEQC2) effort, we perform a cross-platform multi-lab evaluation of eight Pan-Cancer panels to assess best practices for oncopanel sequencing.

Results: All panels demonstrate high sensitivity across targeted high-confidence coding regions and variant types for the variants previously verified to have variant allele frequency (VAF) in the 5-20% range. Sensitivity is reduced by utilizing VAF thresholds due to inherent variability in VAF measurements. Enforcing a VAF threshold for reporting has a positive impact on reducing false positive calls. Importantly, the false positive rate is found to be significantly higher outside the high-confidence coding regions, resulting in lower reproducibility. Thus, region restriction and VAF thresholds lead to low relative technical variability in estimating promising biomarkers and tumor mutational burden.

Conclusion: This comprehensive study provides actionable guidelines for oncopanel sequencing and clear evidence that supports a simplified approach to assess the analytical performance of oncopanels. It will facilitate the rapid implementation, validation, and quality control of oncopanels in clinical use.
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http://dx.doi.org/10.1186/s13059-021-02315-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8051090PMC
April 2021

Effect of quorum-sensing molecule 2-phenylethanol and ARO genes on Saccharomyces cerevisiae biofilm.

Appl Microbiol Biotechnol 2021 May 14;105(9):3635-3648. Epub 2021 Apr 14.

National Engineering Research Center for Biotechnology, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, China.

Biofilms are a form of microbial community that can be beneficial for industrial fermentation because of their remarkable environmental resistance. However, the mechanism of biofilm formation in Saccharomyces cerevisiae remains to be fully explored, and this may enable improved industrial applications for this organism. Although quorum-sensing (QS) molecules are known to be involved in bacteria biofilm formation, few studies have been undertaken with these in fungi. 2-phenylethanol (2-PE) is considered a QS molecule in S. cerevisiae. Here, we found that exogenous 2-PE could stimulate biofilm formation at low cell concentrations. ARO8p and ARO9p are responsible for the synthesis of 2-PE and were crucial to the formation of biofilm. Deletion of the ARO8 and ARO9 genes reduced the content of 2-PE in the early stage of fermentation, reduced ethanol yield and decreased biofilm formation. The expression of FLOp, which is involved in cell adhesion, and the content of extracellular polysaccharides of mutant strains ΔARO8 and ΔARO9 were also significantly reduced. These findings indicate that the production of 2-PE had a positive effect on biofilm formation in S. cerevisiae, thereby providing further key details for studying the formation of biofilm mechanism in the future. KEY POINTS: • Quorum-sensing molecule 2-PE positively affects biofilm formation in S. cerevisiae. • 2-PE synthetic genes ARO8 and ARO9 deletion reduced extracellular polysaccharide. • ARO8 and ARO9 deletion reduced the gene expression of the FLO family.
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http://dx.doi.org/10.1007/s00253-021-11280-4DOI Listing
May 2021

Early rehabilitation after lung transplantation with extracorporeal membrane oxygenation (ECMO) of COVID-19 patient: a case report.

Ann Transl Med 2021 Mar;9(6):512

Department of Rehabilitation Medicine, The First Affiliated Hospital of Zhejiang University, Hangzhou, China.

Even when severe acute respiratory syndrome coronavirus 2-related coronavirus disease 2019 (COVID-19) is treated with first-line drugs, it progresses and leads to irreversible loss of lung function in some critically ill patients, and lung transplantation is an effective treatment for end-stage chronic pulmonary disease. This case report mainly describes the rehabilitation of a 66-year-old female patient with severe COVID-19 after bilateral lung transplantation. The old patient had a body mass index of 31.2 kg/m. She underwent bilateral lung transplantation due to severe and irreversible injury of both lungs. Long-term mechanical ventilation and extracorporeal membrane oxygenation (ECMO) treatment and preoperative and postoperative high-dose corticosteroid therapy and due to the size of the donor lung does not match the size of the recipient's diseased lung, and the right middle lobe of the graft is removed before transplantation. Weaning from the ventilator failed due to weak neuromuscular drive, and muscle strength. A full, personalized pulmonary rehabilitation program was initiated with the help of the physical therapists, the respiratory therapy, the doctors, the nurses and psychotherapist team based on the functional levels. The rehabilitation intervention was conducted on postoperative day 4, This included posture management, airway clearance techniques, respiratory training, muscle strength training, transfer training, daily therapeutic bronchoscopy and psychological support. The ECMO was removed successfully on the fifth day. the patient's physical function, muscle strength and the quality of life has been improved. The good prognosis after rehabilitation indicates that early rehabilitation intervention is effective and feasible and safety for patients after lung transplantation.
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http://dx.doi.org/10.21037/atm-21-456DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039682PMC
March 2021

High-Frequency Oscillations in the Pallidum: A Pathophysiological Biomarker in Parkinson's Disease?

Mov Disord 2021 Apr 13. Epub 2021 Apr 13.

Department of Neurology, University of Minnesota, Minneapolis, Minnesota, USA.

Background: Abnormal oscillatory neural activity in the beta-frequency band (13-35 Hz) is thought to play a role in Parkinson's disease (PD); however, increasing evidence points to alterations in high-frequency ranges (>100 Hz) also having pathophysiological relevance.

Objectives: Studies have found that power in subthalamic nucleus (STN) high-frequency oscillations is increased with dopaminergic medication and during voluntary movements, implicating these brain rhythms in normal basal ganglia function. The objective of this study was to investigate whether similar signaling occurs in the internal globus pallidus (GPi), a nucleus increasingly used as a target for deep brain stimulation (DBS) for PD.

Methods: Spontaneous and movement-related GPi field potentials were recorded from DBS leads in 5 externalized PD patients on and off dopaminergic medication, as well as from 3 rhesus monkeys before and after the induction of parkinsonism with the neurotoxin 1-methyl-4-phenyl-1,2,3,6 tetrahydropyridine.

Results: In the parkinsonian condition, we identified a prominent oscillatory peak centered at 200-300 Hz that increased during movement. In patients the magnitude of high-frequency oscillation modulation was negatively correlated with bradykinesia. In monkeys, high-frequency oscillations were mostly absent in the naive condition but emerged after the neurotoxin 1-methyl-4-phenyl-1,2,3,6 tetrahydropyridine. In patients, spontaneous high-frequency oscillations were significantly attenuated on-medication.

Conclusions: Our findings provide evidence in support of the hypothesis that exaggerated, movement-modulated high-frequency oscillations in the GPi are pathophysiological features of PD. These findings suggest that the functional role(s) of high-frequency oscillations may differ between the STN and GPi and motivate additional investigations into their relationship to motor control in normal and diseased states.
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http://dx.doi.org/10.1002/mds.28566DOI Listing
April 2021

A novel fluorescent strategy based on double modifications of metal organic framework material CAU-10-NH for low background and high sensitivity determination of HS.

Talanta 2021 Jul 6;229:122271. Epub 2021 Mar 6.

School of Chemistry, Guangzhou Key Laboratory of Analytical Chemistry for Biomedicine, South China Normal University, Guangzhou, 510006, PR China.

Hydrogen sulfide is typical metabolic marker and environmental pollutant which is worthwhile to determine. Herein, a low background and high sensitivity fluorescent strategy based on double modifications of metal organic framework material CAU-10-NH is proposed for the determination of hydrogen sulfide. Firstly, a functional monomer 3,5-diaminobenzoic acid is employed to modify on the CAU-10-NH, the product CAU-10-NH-dAba has strong fluorescent performance at 412 nm under an excitation wavelength of 320 nm. Subsequently, it is further modified by the azide group to form CAU-10-NH-dAba-N. This azidation inhibits the fluorescent signal. However, in the presence of hydrogen sulfide, the azide group is specifically reduced to amidogen, and results in the recovery of the fluorescence. The CAU-10-NH-DABA-N was characterized by solid state NMR, XPS, fluorescence, IR, XRD, SEM and specific surface area. After the optimization of pH value, temperature and interaction time, the detection results of hydrogen sulfide demonstrate the linear range of this strategy is from 20 to 140 nM with a detection limit of 1.51 nM, which is significantly better than that of the CAU-10-NH merely modified by 3,5-dinitrobenzoic acid. Meanwhile, the satisfactory assay results of hydrogen sulfide in serum sample and Pearl river water suggest a potential application prospect of this strategy in clinical diagnosis and environment monitoring.
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http://dx.doi.org/10.1016/j.talanta.2021.122271DOI Listing
July 2021