Publications by authors named "Ying Yang"

2,660 Publications

  • Page 1 of 1

Roles of stress response-related signaling and its contribution to the toxicity of zearalenone in mammals.

Compr Rev Food Sci Food Saf 2022 Jun 24. Epub 2022 Jun 24.

State Key Laboratory of Animal Nutrition, Department of Companion Animal Science, College of Animal Science and Technology, China Agricultural University, Beijing, P. R. China.

Zearalenone (ZEA) is a mycotoxin frequently found in cereal crops and cereal-derived foodstuffs worldwide. It affects plant productivity, and is also a serious hazard to humans and animals if being exposed to food/feed contaminated by ZEA. Studies over the last decade have shown that the toxicity of ZEA in animals is mainly mediated by the various stress responses, such as endoplasmic reticulum (ER) stress, oxidative stress, and others. Accumulating evidence shows that oxidative stress and ER stress signaling are actively implicated in and contributes to the pathophysiology of various diseases. Biochemically, the deleterious effects of ZEA are associated with apoptosis, DNA damage, and lipid peroxidation by regulating the expression of genes implicated in these biological processes. Despite these findings, the underlying mechanisms responsible for these alterations remain unclear. This review summarized the characteristics, metabolism, toxicity and the deleterious effects of ZEA exposure in various tissues of animals. Stress response signaling implicated in the toxicity as well as potential therapeutic options with the ability to reduce the deleterious effects of ZEA in animals were highlighted and discussed.
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http://dx.doi.org/10.1111/1541-4337.12974DOI Listing
June 2022

Contrasting Responses of Multispatial Soil Fungal Communities of Thuja sutchuenensis Franch., an Extremely Endangered Conifer in Southwestern China.

Microbiol Spectr 2022 Jun 23:e0026022. Epub 2022 Jun 23.

Center for Biodiversity Conservation and Utilization, School of Life Sciences, Southwest Universitygrid.263906.8, Beibei, Chongqing, China.

Thuja sutchuenensis Franch. is an endangered species in southwest China, distributed sporadically in mountainous areas. Soil property and soil fungal community play a crucial role in plant growth and survival. Nevertheless, understanding soil properties and the soil fungal community in the areas where is distributed is extremely limited. Hence, this study collected a total of 180 soil samples from five altitudinal distribution areas (altitudinal gradients) and three vertical depths throughout four horizontal distances from the base of each tree. The results found that altitudinal gradients and vertical depths altered soil properties, including pH, organic matter content, water content, total nitrogen, phosphorus, and potassium, and available nitrogen, phosphorus, and potassium. The fungal alpha diversity indexes (Chao1 and Shannon) and beta diversity were dramatically decreased with elevation. In addition, high altitudes (2,119 m) harbored the highest relative abundance of ectomycorrhizal fungi (27.57%) and the lowest relative abundance of plant-pathogenic fungi (1.81%). Meanwhile, we identified a series of fungal communities, such as , , , , and Boletaceae, that play an essential role in the survival of . The correlation analysis and random forest model identified that water content and total phosphorus showed strong relationships with fungal characteristics and were the primary variables for Zygomycota and Rozellomycota. Collectively, the findings of this integrated analysis provide profound insights into understanding the contrasting responses of soil fungal communities and provide a theoretical basis for habitat restoration and species conservation from multispatial perspectives. The present study highlights the importance of fungal communities in an endangered plant, . Comparative analysis of soil samples in nearly all extant populations identified that soil properties, especially soil nutrients, might play critical roles in the survival of . Our findings prove that a series of fungal communities (e.g., , , and ) could be key indicators for survival. In addition, this is the first time that large-scale soil property and fungal community investigations have been carried out in southwest China, offering important values for exploring the distribution pattern of regional soil microorganisms. Collectively, our findings display a holistic picture of soil microbiome and environmental factors associated with .
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http://dx.doi.org/10.1128/spectrum.00260-22DOI Listing
June 2022

Soil Microbial Network Complexity Varies With pH as a Continuum, Not a Threshold, Across the North China Plain.

Front Microbiol 2022 6;13:895687. Epub 2022 Jun 6.

School of Geography and Ocean Science, Nanjing University, Nanjing, China.

There has been little study on the biogeographical patterns of microbial co-occurrence, especially in agricultural soils. Here we investigated the biogeographical patterns and major drivers of co-occurrence network topological structure, and the relative abundance of keystone taxa for soil bacterial and fungal communities using high-throughput sequencing on a set of 90 samples across a 1,092 km transect in wheat fields of the North China Plain (NCP). We found that pH was the most important environmental factor driving network topology and relative abundance of keystone taxa. For the metacommunity composed of both bacteria and fungi, and for the bacterial community alone, lower soil pH was associated with a more complex microbial network. However, the network for fungi showed no strong trend with soil pH. In addition, keystone taxa abundance was positively correlated with ecosystem function and stability, and best explained by pH. Our results present new perspectives on impacts of pH on soil microbial network structure across large scales in agricultural environments. This improved knowledge of community processes provides a step toward understanding of functioning and stability of agricultural ecosystems.
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http://dx.doi.org/10.3389/fmicb.2022.895687DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207804PMC
June 2022

Zn Cross-Linked Alginate Carrying Hollow Silica Nanoparticles Loaded with RL-QN15 Peptides Provides Promising Treatment for Chronic Skin Wounds.

ACS Appl Mater Interfaces 2022 Jun 22. Epub 2022 Jun 22.

Department of Anatomy and Histology & Embryology, Faculty of Basic Medical Science, Kunming Medical University, Kunming, Yunnan 650500, China.

Chronic and non-healing wounds pose a great challenge to clinical management and patients. Many studies have explored novel interventions against skin wounds, with bioactive peptides, nanoparticles, and hydrogels arousing considerable attention regarding their therapeutic potential. In this study, the prohealing peptide RL-QN15 was loaded into hollow silica nanoparticles (HSNs), with these [email protected] nanocomposites then combined with zinc alginate (ZA) gels to obtain [email protected]/ZA hydrogel. The characteristics, biological properties, and safety profiles of the hydrogel composites were then evaluated. Results showed that the hydrogel had good porosity, hemocompatibility, biocompatibility, and broad-spectrum antimicrobial activity, with the slow release of loaded RL-QN15. Further analysis indicated that the hydrogel promoted skin cell proliferation and keratinocyte scratch repair, regulated angiogenesis, reduced inflammation, and accelerated re-epithelialization and granulation tissue formation, resulting in the rapid healing of both full-thickness skin wounds and methicillin-resistant biofilm-infected chronic wounds in mice. This peptide-based hydrogel provides a novel intervention for the treatment of chronic skin wounds and shows promise as a wound dressing in the field of tissue regeneration.
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http://dx.doi.org/10.1021/acsami.2c03583DOI Listing
June 2022

Resveratrol alleviates enterotoxigenic K88-induced damage by regulating SIRT-1 signaling in intestinal porcine epithelial cells.

Food Funct 2022 Jun 22. Epub 2022 Jun 22.

State Key Laboratory of Animal Nutrition, Department of Animal Nutrition and Feed Science, China Agricultural University, Beijing 100193, China.

This study found that resveratrol pretreatment attenuated porcine intestinal epithelial cell damage caused by enterotoxigenic (ETEC) K88 and the protective effects of resveratrol were associated with SIRT-1 signaling. ETEC K88 is a main intestinal pathogen for post-weaning diarrhea (PWD) in piglets. With the strict ban on antibiotics in animal feed, people are seeking effective antibiotic substitutes to protect the intestinal system against harmful pathogenic bacteria. This study was conducted to evaluate the effects of resveratrol, a natural plant polyphenol, on ETEC K88-induced cellular damage in porcine enterocytes and underlying mechanisms. Intestinal porcine epithelial cell line 1 (IPEC-1) cells, pretreated with or without resveratrol (30 μM, 4 h), were challenged with ETEC K88 (MOI = 1 : 10) for 3 h. The results showed that ETEC K88 infection induced severe damage and dysfunction in IPEC-1 cells, as evidenced by a reduced cell viability, decreased tight junctions, mitochondrial dysfunction, and autophagy. It is noteworthy that IPEC-1 cells pre-treated with resveratrol improved their capacity for resistance to most of these abnormal phenotypes caused by ETEC K88 infection. Furthermore, we found that the activation of SIRT-1 signaling was associated with the benefits of resveratrol, as demonstrated by EX-527, an inhibitor of SIRT-1, which reversed most of the protective effects of resveratrol. In conclusion, these results indicated that resveratrol could protect intestinal epithelial cells against ETEC K88 infection by activating SIRT-1 signaling. These findings provide new insights into the role of resveratrol in maintaining intestinal physiological functions.
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http://dx.doi.org/10.1039/d1fo03854kDOI Listing
June 2022

A map of the spatial distribution and tumour-associated macrophage states in glioblastoma and grade-4 IDH-mutant astrocytoma.

J Pathol 2022 Jun 20. Epub 2022 Jun 20.

Institute of Pathology and Southwest Cancer Centre, Southwest Hospital, Third Military Medical University (Army Medical University) and Key Laboratory of Tumour Immunopathology, Ministry of Education of China, Chongqing, PR China.

Tumour-associated macrophages (TAMs) abundantly infiltrate high-grade gliomas and orchestrate immune response, but their diversity in isocitrate dehydrogenase (IDH)-differential grade-4 gliomas remains largely unknown. This study aimed to dissect the transcriptional states, spatial distribution and clinicopathological significance of distinct monocyte-derived TAM (Mo-TAM) and microglia-derived TAM (Mg-TAM) clusters across glioblastoma-IDH-wildtype and astrocytoma-IDH-mutant-grade 4 (Astro-IDH-mut-G4). Single-cell RNA sequencing was performed on four cases of human glioblastoma and three cases of Astro-IDH-mut-G4. Cell clustering, single-cell regulatory network inference and gene set enrichment analysis were performed to characterize the functional states of myeloid clusters. Spatial distribution of TAM subsets was determined in human glioma tissues using multiplex immunostaining. The prognostic value of different TAM-cluster specific geneset was evaluated in the TCGA glioma cohort. Profiling and unbiased clustering of 24,227 myeloid cells from glioblastoma and Astro-IDH-mut-G4 identified 9 myeloid cell clusters including monocyte, six Mo/Mg-TAM subsets, dendritic cell, and proliferative myeloid cluster. Different Mo/Mg-TAM clusters manifest functional and transcriptional diversity controlled by specific regulons. Multiplex immunostaining of subset-specific markers identified spatial enrichment of distinct TAM clusters at peri-vascular/necrotic areas in tumour parenchyma or at tumour-brain interface. Glioblastoma harboured a substantially higher number of monocytes and Mo-TAM-inflammatory cluster, whereas Astro-IDH-mut-G4 was with higher proportion of TAM subset mediating antigen presentation. Glioblastomas with higher proportion of monocytes exhibited a mesenchymal signature, increased angiogenesis and worse patient outcome. Our findings provide insight into myeloid cell diversity and its clinical relevance in IDH-differential grade-4 gliomas, and may serve as a resource for immunotherapy development. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/path.5984DOI Listing
June 2022

Future Prospect of Oral Microbiota Influencing Allergy/Asthma Development.

Front Microbiol 2022 30;13:875664. Epub 2022 May 30.

Department of Endocrinology, Affiliated Hospital of Yunnan University, Kunming, China.

Allergic diseases have become a primary public health issue in a moderately prosperous society. Colonization of microorganisms early in life appears to be significant in guiding the regulation of childhood immune system maturation and allergy development. Since the oral cavity is the first position where most foreign antigens meet the immune system, the oral microbiota may play a key role in the development of allergies. However, the study on the effects of oral microorganisms on allergy/asthma is very restricted and should be actively investigated. It requires considerable effort to enrich our knowledge in this area of the relationship between the oral cavity and allergy/asthma. To promote the rapid progress of relevant research. In this review, we aimed to provide several insights into the role of the oral microbiota in allergy/asthma while prospecting future directions.
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http://dx.doi.org/10.3389/fmicb.2022.875664DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9198628PMC
May 2022

mA Modification Involves in Enriched Environment-Induced Neurogenesis and Cognition Enhancement.

Front Cell Dev Biol 2022 2;10:903179. Epub 2022 Jun 2.

The Children's Hospital, National Clinical Research Center for Child Health, School of Medicine, Zhejiang University, Hangzhou, China.

Although previous studies have shown that an enriched environment (EE) promotes neurogenesis and alters DNA and histone modifications, it remains largely unknown whether an EE affects epitranscriptome in the context of neuronal development. Here, we showed that EE exposure enhanced the pool of adult neural stem/progenitor cells (aNSPCs) and promoted neuronal differentiation of aNSPCs. EE exposure also improved cognitive capabilities and altered the expression of genes relating to neuronal development, neurogenesis, and memory. -Methyladenosine (mA) immunoprecipitation combined with deep sequencing (MeRIP-seq) data analysis revealed that EE exposure increased the global level of mA and led to differential mA mRNA modification. Differential mA modification-associated genes are involved in neuronal development, neurogenesis, and so on. Notably, EE exposure decreased the protein level of mA eraser Fto, but did not affect the protein level of mA writers METTL3 and METTL14. Taken together, our results suggest that enriched environment exposure induces differential mA mRNA modification and adds a novel layer to the interaction between the environment and epigenetics in the context of postnatal neuronal development.
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http://dx.doi.org/10.3389/fcell.2022.903179DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201454PMC
June 2022

Dual nicotinic acetylcholine receptor subunit gene knockouts reveal limits to functional redundancy.

Pestic Biochem Physiol 2022 Jun 11;184:105118. Epub 2022 May 11.

Bio21 Molecular Science and Biotechnology Institute, School of BioSciences, The University of Melbourne, Parkville 3010, Australia. Electronic address:

The nicotinic acetylcholine receptor (nAChR) subunit gene family consists of ten members in Drosophila melanogaster. The mature nAChR is a pentamer assembled from these subunits. Despite recent advances in the in vitro expression of some receptor subunit combinations (nAChR subtypes), the in vivo combinations and stoichiometry of these subtypes remains poorly defined. In addition, there are many potential nAChR signalling roles for different subtypes in insect behaviour, development and physiology. Prior work has shown that nAChR subunit mutants can display altered sleep and mating behaviour, disrupted hormone signalling and reduced locomotion, climbing ability and longevity. Teasing out the specific receptor subunits that are involved in these different functions is potentially made more difficult given that the structural similarity between members of gene families often means that there is a degree of functional redundancy. In order to circumvent this, we created a dual knockout strain for the Dα1 and Dβ2 nAChR subunit genes and examined four traits including insecticide resistance. These subunits had been previously implicated in the response to a neonicotinoid insecticide, imidacloprid. The use of the dual knockout revealed that Dα1 and Dβ2 subunits are involved in signalling that leads to the inflation of wings following adult emergence from the pupal case. The Dβ1 subunit had previously been implicated as a contributor to this function. The lack of a phenotype or low penetrance of the phenotype in the Dα1 and Dβ2 single mutants compared to the dual knockout suggests that these subunits are, to some extent, functionally redundant. We also observed stronger reductions in climbing ability and longevity in the dual knockout. Our findings demonstrate that a dual knockout approach to examining members of the nAChR subunit gene family may increase the power of genetic approaches linking individual subunits and combinations thereof to particular biological functions. This approach will be valuable as the nAChRs are so widely expressed in the insect brain that they are likely to have many functions that hereto remain undetected.
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http://dx.doi.org/10.1016/j.pestbp.2022.105118DOI Listing
June 2022

A Novel Fluorescent Dye Extracted from for Labeling Mitochondria after Fixation.

Scanning 2022 3;2022:7486005. Epub 2022 Jun 3.

Department of Oral Pathology, School of Dentistry, Chonnam National University, Gwangju 61186, Republic of Korea.

Mitochondria are versatile organelles and function by communicating with cellular ecosystems. The fluorescent colocalization analysis after fixation is a highly intuitive method to understand the role of mitochondria. However, there are few fluorescent dyes available for mitochondrial staining after fixation. In this study, a novel fluorescent dye (BO-dye), extracted from , was applied for mitochondrial staining in fixed immortalized human oral keratinocytes. The BO-dye (excitation: 414 nm, emission: 677 nm) is a small fluorescent molecular dye, which can cross the cytomembrane without permeabilization. We assume that the BO-dye could aggregate and bind to the mitochondria stably. BO-dye exhibited a mega-Stokes shift (>250 nm), which is an important feature that could reduce self-quenching and enhance the signal-to-noise ratio. Analysis of photophysical properties revealed that the BO-dye is temperature and pH insensitive, and it exhibits superior photostability. These results indicate that BO-dye can be considered an alternative fluorescent dye for labeling mitochondria after fixation.
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http://dx.doi.org/10.1155/2022/7486005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9187463PMC
June 2022

Insights into diet-associated oxidative pathomechanisms in inflammatory bowel disease and protective effects of functional amino acids.

Nutr Rev 2022 Jun 15. Epub 2022 Jun 15.

are with the State Key Laboratory of Animal Nutrition, Department of Animal Nutrition and Feed Science, China Agricultural University, Beijing, China.

There has been a substantial rise in the incidence and prevalence of clinical patients presenting with inflammatory bowel disease (IBD), which includes Crohn's disease and ulcerative colitis. Accumulating evidence has corroborated the view that dietary factors (particularly diets with high levels of saturated fat or sugar) are involved in the development and progression of IBD, which is predominately associated with changes in the composition of the gut microbiota and an increase in the generation of reactive oxygen species. Notably, the ecological imbalance of the gut microbiome exacerbates oxidative stress and inflammatory responses, leading to perturbations of the intestinal redox balance and immunity, as well as mucosal integrity. Recent findings have revealed that functional amino acids, including L-glutamine, glycine, L-arginine, L-histidine, L-tryptophan, and hydroxyproline, are effectively implicated in the maintenance of intestinal redox and immune homeostasis. These amino acids and their metabolites have oxygen free-radical scavenging and inflammation-relieving properties, and they participate in modulation of the microbial community and the metabolites in the gut. The principal focus of this article is a review of recent advances in the oxidative pathomechanisms of IBD development and progression in relation to dietary factors, with a particular emphasis on the redox and signal transduction mechanisms of host cells in response to unbalanced diets and enterobacteria. In addition, an update on current understanding of the protective effects of functional amino acids against IBD, together with the underlying mechanisms for this protection, have been provided.
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http://dx.doi.org/10.1093/nutrit/nuac039DOI Listing
June 2022

Emerging Role of Epitranscriptomics in Diabetes Mellitus and Its Complications.

Front Endocrinol (Lausanne) 2022 27;13:907060. Epub 2022 May 27.

Department of Endocrinology, The Affiliated Hospital of Yunnan University and the Second People's Hospital of Yunnan Province, Kunming, China.

Diabetes mellitus (DM) and its related complications are among the leading causes of disability and mortality worldwide. Substantial studies have explored epigenetic regulation that is involved in the modifications of DNA and proteins, but RNA modifications in diabetes are still poorly investigated. In recent years, posttranscriptional epigenetic modification of RNA (the so-called 'epitranscriptome') has emerged as an interesting field of research. Numerous modifications, mainly -methyladenosine (mA), have been identified in nearly all types of RNAs and have been demonstrated to have an indispensable effect in a variety of human diseases, such as cancer, obesity, and diabetes. Therefore, it is particularly important to understand the molecular basis of RNA modifications, which might provide a new perspective for the pathogenesis of diabetes mellitus and the discovery of new therapeutic targets. In this review, we aim to summarize the recent progress in the epitranscriptomics involved in diabetes and diabetes-related complications. We hope to provide some insights for enriching the understanding of the epitranscriptomic regulatory mechanisms of this disease as well as the development of novel therapeutic targets for future clinical benefit.
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http://dx.doi.org/10.3389/fendo.2022.907060DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9184717PMC
June 2022

Dihydromyricetin ameliorates diet-induced obesity and promotes browning of white adipose tissue by upregulating IRF4/PGC-1α.

Nutr Metab (Lond) 2022 Jun 11;19(1):38. Epub 2022 Jun 11.

Department of Endocrinology, Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Background: Promoting the browning of white adipose tissue (WAT) is a promising approach for the treatment of obesity and related comorbidities because it increases energy expenditure. In this study, we investigated whether Dihydromyricetin (DHM), a flavonoid component, could ameliorate diet-induced obesity through promoting the browning of WAT.

Methods: Male C57BL/6 J mice were received a high-fat diet (HFD) to induce obesity and subsequently were treated with DHM (100 mg/kg/day) or vehicle for 4 weeks. The effects of DHM on weight reduction and metabolic phenotype improvement were observed in the mice. The expression of genes and protein involved in browning of WAT were assessed in inguinal WAT (iWAT) of the mice. Then, the effect of DHM on the inducing browning program was verified in adipocytes differentiated from stromal vascular fraction (SVF) cells of mouse iWAT. Finally, the mechanism by which DHM improves the browning of WAT was explored using RNA-seq and luciferase reporter assay.

Results: We find that DHM reduces body weight, decreases WAT mass, improves glucose and lipid metabolic disorders, and ameliorates hepatic steatosis in diet-induced obese (DIO) mice. Further studies show that DHM induces WAT browning, which is manifested by increased expression of uncoupling protein 1 (UCP1) and peroxisome proliferator-activated receptor gamma coactivator (PGC)-1α and enhanced mitochondrial activity in iWAT and primary adipocytes. In addition, we also find that DHM enhances interferon regulatory factor 4 (IRF4) expression, which is a key transcriptional regulator of PGC-1α.

Conclusion: Our findings identify that DHM prevents obesity by inducing the browning of WAT through the upregulation of IRF4/PGC-1α, which may have potential therapeutic implications for the treatment of obesity.
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http://dx.doi.org/10.1186/s12986-022-00672-6DOI Listing
June 2022

Nitrogen-doped graphene-poly(hydroxymethylated-3,4-ethylenedioxythiophene) nanocomposite electrochemical sensor for ultrasensitive determination of luteolin.

RSC Adv 2022 May 23;12(24):15517-15525. Epub 2022 May 23.

College of Mechanical and Electrical Engineering, Jiaxing University Jiaxing 314001 PR China

An ultrasensitive luteolin electrochemical sensor was constructed by co-electropolymerization of nitrogen-doped graphene (N-GR) and hydroxymethylated-3,4-ethylenedioxythiophene (EDOT-MeOH) using cyclic voltammetry (CV). Because of the synergistic effects of the large surface area, superior electrical conductivity, and large amount of chemically active sites of N-GR together with the satisfactory water solubility and high conductivity of poly(hydroxymethylated-3,4-ethylenedioxythiophene) (PEDOT-MeOH), the N-GR-PEDOT-MeOH nanocomposite sensor exhibited high electrochemical sensitivity towards luteolin with a wide linear range of 0.005-10.06 μM and low detection limit of 0.05 nM. Satisfactory reproducibility, selectivity, and stability were exhibited by this electrochemical sensor. Additionally, the proposed sensor was employed for trace-level analysis of luteolin in actual samples of herbal medicines (thyme ( L.), honeysuckle ( Thunb.), and Tibetan Duyiwei ( (Benth.) Kudo)) with satisfactory results.
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http://dx.doi.org/10.1039/d2ra01669aDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9125232PMC
May 2022

Multi-Party Cryptographic Key Distribution Protocol over a Public Network Based on a Quick-Response Code.

Sensors (Basel) 2022 May 25;22(11). Epub 2022 May 25.

Center for Quantum Technology Research, School of Physics, Beijing Institute of Technology, Beijing 100081, China.

In existing cryptographic key distribution (CKD) protocols based on computational ghost imaging (CGI), the interaction among multiple legitimate users is generally neglected, and the channel noise has a serious impact on the performance. To overcome these shortcomings, we propose a multi-party interactive CKD protocol over a public network, which takes advantage of the cascade ablation of fragment patterns (FPs). The server splits a quick-response (QR) code image into multiple FPs and embeds different "watermark" labels into these FPs. By using a CGI setup, the server will acquire a series of bucket value sequences with respect to different FPs and send them to multiple legitimate users through a public network. The users reconstruct the FPs and determine whether there is an attack in the public channel according to the content of the recovered "watermark" labels, so as to complete the self-authentication. Finally, these users can extract their cryptographic keys by scanning the QR code (the cascade ablation result of FPs) returned by an intermediary. Both simulation and experimental results have verified the feasibility of this protocol. The impacts of different attacks and the noise robustness have also been investigated.
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http://dx.doi.org/10.3390/s22113994DOI Listing
May 2022

Polysaccharides Better Regulate the Microbiota of Women Than Men.

Foods 2022 Jun 2;11(11). Epub 2022 Jun 2.

Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.

is widely used as a health supplement, but its specific impact on healthy gut microbiota has not yet been clarified, nor has its impact on different human genders. To overcome the problems mentioned above. DOP was extracted and purified with an 8000-12,000 Da dialysis bag. The molecular weight and monosaccharide composition were determined using HPGPC and GC. Gas chromatography was used to detect the content of SCFA. 16S rDNA sequencing was used to analyze the diversity of human microbiota. The results showed that DOP contained two fractions, with an average molecular weight of 277 kDa and 1318 Da, and mainly composed of mannose and glucose. DOP can increase the relative abundance of benign microbiota and decrease the harmful types. Propionic acid content in women was significantly increased after DOP treatment. Finally, the correlation analysis revealed that DOP was beneficial to the microbiota of both men and women. It can be concluded from the results that DOP is a health supplement suitable for humans, and especially women.
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http://dx.doi.org/10.3390/foods11111641DOI Listing
June 2022

Effect of radiofrequency catheter ablation on left atrial structure and function in patients with different types of atrial fibrillation.

Sci Rep 2022 Jun 9;12(1):9511. Epub 2022 Jun 9.

Division of Cardiology, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, People's Republic of China.

Radiofrequency catheter ablation (RFCA) is widely used to treat atrial fibrillation (AF), but its effect on left atrial (LA) remodeling in patients with AF is not completely clarified. Few studies have reported the changes in structure and function of the left atrium in patients with different types of AF after RFCA. To analyze the effect of RFCA on the LA structure and function in patients with nonvalvular paroxysmal AF, persistent AF and long-standing persistent AF (LSPAF). RFCA was performed in 180 patients with paroxysmal AF, persistent AF and LSPAF. The changes of LA structure and function in echocardiogram and speckle-tracking echocardiography findings were compared before the procedure, and at 1, 2, 3, 4 weeks, and 2, 3, 6, and 9-12 months after the procedure. There were 60 patients in the paroxysmal AF group, 60 in the persistent AF group and 60 patients in LSPAF group. The pre-procedure LA diameter and volume were smaller in the paroxysmal AF group than persistent AF and LSPAF group. There was no significant change of in the LA structure and function in the paroxysmal AF group within 1 year. In the persistent AF and LSPAF groups, LA structure (anteroposterior diameter, LA volume) significantly decreased, but remained larger than that in paroxysmal AF group. In persistent and LSPAF, function (LA ejection fraction, strain, strain rate) increased significantly within 1 week, then gradually increased. RFCA improved the LA structure and function and resulted in heart reverse remodeling, especially for persistent AF and LSPAF.
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http://dx.doi.org/10.1038/s41598-022-13725-wDOI Listing
June 2022

The Accuracy and Clinical Relevance of the Multi-echo Dixon Technique for Evaluating Changes to Hepatic Steatosis in Patients with Non-alcoholic Fatty Liver Disease Treated with Formulated Food.

Magn Reson Med Sci 2022 Jun 7. Epub 2022 Jun 7.

Department of Radiology, Hangzhou Normal University Affiliated Hospital.

Purpose: The Multi-echo Dixon (ME-Dixon) is a non-invasive quantitative MRI technique to diagnose non-alcoholic fatty liver disease (NAFLD). In this study, the hydrogen proton MR spectroscopy (H-MRS) was used as a reference to explore the accuracy of the ME-Dixon technique in evaluating hepatic steatosis in NAFLD patients after ingesting formulated food and its correlation with changes in clinical indicators.

Methods: Twenty-seven patients with NAFLD were enrolled. Fifteen patients completed 12 weeks of treatment with prebiotics and dietary fiber. In addition, abdominal MRI scans and blood tests were performed before and after treatment. The MRI-proton density fat fraction (MRI-PDFF) and MRS-PDFF were measured using the ME-Dixon and H-MRS techniques. The Bland-Altman method and Pearson correlation analysis were used to test the consistency of the two techniques for measuring the liver fat content and the changed values. Besides, correlation analysis was conducted between the MRI-PDFF value and metabolic indicators.

Results: In the PDFF quantification of 42 person-times and the monitoring of the PDFF change in 15 patients under treatment, there was a good consistency and a correlation between MRI and MRS. At baseline, MRI-PDFF was positively correlated with insulin resistance index (HOMA-IR), fatty liver index (FLI), and liver enzymes. After treatment, the changes in MRI-PDFF were positively correlated with the recovery degree of FLI and liver enzymes.

Conclusion: ME-Dixon has a good consistency and a correlation with MRS in quantifying the liver fat content and monitoring the treatment effect, which may be used as an accurate indicator for clinical monitoring of changes in the liver fat content.
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http://dx.doi.org/10.2463/mrms.mp.2021-0168DOI Listing
June 2022

Rapid analysis of differential chemical compositions of using thin-layer chromatography spray ionization-mass spectrometry.

Analyst 2022 Jun 8. Epub 2022 Jun 8.

The MOE Key Laboratory of Standardization of Chinese Medicines, The SATCM Key Laboratory of New Resources and Quality Evaluation of Chinese Medicines, The Shanghai Key Laboratory for Compound Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.

, with medicinal and edible properties, contains multiple chemical components that pose difficulties in its quality control. Herein, we established a simple thin-layer chromatography spray ionization-mass spectrometry (TLCSI-MS) device that allows the simultaneous separation and identification of bioactive compounds. The triterpene acids in different medicinal parts of were characterized, and the markers of differences were revealed. The quantitative analysis of its pharmaceutical preparation has also been performed to verify the feasibility. The study provides a new analytical perspective for the screening and identification of multiple compounds and a good option for the quality evaluation of herbal medicines and foods.
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http://dx.doi.org/10.1039/d2an00565dDOI Listing
June 2022

Evaluation of a Novel Simulation Curriculum With the Segmented Model in Pediatric Cardiovascular Education.

Front Public Health 2022 20;10:887405. Epub 2022 May 20.

Education Office, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, China.

Objective: The need to develop the full range of knowledge, skills, and professionalism poses new challenges for pediatric cardiovascular education. This study aimed to investigate the effectiveness of a novel simulation curriculum with the segmented model for pediatric cardiovascular residents.

Materials And Methods: First, the simulation course was designed according to a prior survey and based on a human patient simulator setting. Then, another 55 residents were randomly selected and assigned to participate in a simulation course (about acute fulminant myocarditis in children), either in the experimental group or the control group. Taking full advantage of the simulation education, the simulation case in the experimental group was divided into three segments and included a micro-debriefing at the end of each segment. The three segments were independent but together formed the whole case. It was designed through three cycles of running and debriefing, and more challenging tasks were gradually proposed to residents. The case in the control group was not split and included only one case running and debriefing. The assessments of the residents' knowledge, skills, professionalism performance, and satisfaction feedback from residents were analyzed to evaluate the effectiveness of the course.

Results: In total, 44 residents completed the whole experimental period, including 23 participants in the experimental group and 21 participants in the control group. The pre-course knowledge assessment scores of the two groups were comparable, while the mean post-course score in the experimental group was 82.61 ± 17.38, which was significantly higher than that in the control group (50.48 ± 18.57, < 0.01). The mean skills assessment score of residents in the experimental group was 84.17 ± 6.01, which was significantly higher than the control group (54.50 ± 5.72, < 0.01). In terms of the professionalism assessment, the residents in the experimental group achieved better performance than those in the control group in all aspects (respect, privacy, communication, responsibility, and cooperation) ( < 0.05). Satisfaction feedback from residents showed that self-confidence regarding knowledge mastery in the experimental group was significantly higher than that in the control group ( < 0.05), while there were no significant differences in the evaluations of the teacher's performance ( > 0.05).

Conclusions: The novel simulation curriculum with the segmented model helps residents achieve better performance in terms of knowledge, skills, and professionalism while improving self-confidence. It has some value in pediatric cardiovascular education and is worthy of further promotion.
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http://dx.doi.org/10.3389/fpubh.2022.887405DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9163956PMC
June 2022

Lanthanum nitrate as aqueous electrolyte additive for favourable zinc metal electrodeposition.

Nat Commun 2022 Jun 6;13(1):3252. Epub 2022 Jun 6.

State Key Laboratory of Material Processing and Die & Mold Technology, School of Materials Science and Engineering, Huazhong University of Science and Technology, Wuhan, Hubei Province, 430074, China.

Aqueous zinc batteries are appealing devices for cost-effective and environmentally sustainable energy storage. However, the zinc metal deposition at the anode strongly influences the battery cycle life and performance. To circumvent this issue, here we propose the use of lanthanum nitrate (La(NO)) as supporting salt for aqueous zinc sulfate (ZnSO) electrolyte solutions. Via physicochemical and electrochemical characterizations, we demonstrate that this peculiar electrolyte formulation weakens the electric double layer repulsive force, thus, favouring dense metallic zinc deposits and regulating the charge distribution at the zinc metal|electrolyte interface. When tested in Zn||VS full coin cell configuration (with cathode mass loading of 16 mg cm), the electrolyte solution containing the lanthanum ions enables almost 1000 cycles at 1 A g (after 5 activation cycles at 0.05 A g) with a stable discharge capacity of about 90 mAh g and an average cell discharge voltage of ∼0.54 V.
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http://dx.doi.org/10.1038/s41467-022-30939-8DOI Listing
June 2022

Association between polymorphisms of ABCB1 and prognosis in esophageal squamous cell carcinoma patients treated with taxane.

J Gene Med 2022 Jun 4:e3434. Epub 2022 Jun 4.

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education, China), The VIP-II Gastrointestinal Cancer Division of Medical Department, Peking University Cancer Hospital & Institute, Beijing, China.

Background: In China, most esophageal cancer patients are squamous cell carcinomas and are treated with taxane-containing regimens; however, few studies have examined taxane pharmacokinetics genes and esophageal squamous cell carcinoma (ESCC) prognosis.

Methods: In total, 227 pathologically confirmed ESCC patients receiving chemotherapy with taxane were included in the analysis. We genotyped seven SNPs (rs1045642, rs2032582 and rs3213619 of ABCB1; rs2231137 and rs2231142 of ABCG2; and ABCC1 rs246221 and ABCC2 rs3740066) and analyzed their relationship with overall survival.

Results: With a retrospective cohort study design, by Cox regression and semi-Bayesian shrinkage, in the genetic recessive model, the variant homozygote of ABCB1 rs1045642 was inversely associated with survival (semi-Bayesian shrinkage crude hazard ratio = 1.82, 95% confidence interval = 1.00, 3.31; p = 0.0482).

Conclusions: Because of inherent defects of the research itself, the finding that the ABCB1 rs1045642 variant was related to poor prognosis in ESCC patients treated with taxane-containing regimens needs to be tested in a larger population and by using more genetic and molecular mechanism experiments.
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http://dx.doi.org/10.1002/jgm.3434DOI Listing
June 2022

Phase separation of Ddx3xb helicase regulates maternal-to-zygotic transition in zebrafish.

Cell Res 2022 Jun 3. Epub 2022 Jun 3.

CAS Key Laboratory of Genomic and Precision Medicine, Collaborative Innovation Center of Genetics and Development, College of Future Technology, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China.

Vertebrate embryogenesis involves a conserved and fundamental process, called the maternal-to-zygotic transition (MZT), which marks the switch from a maternal factors-dominated state to a zygotic factors-driven state. Yet the precise mechanism underlying MZT remains largely unknown. Here we report that the RNA helicase Ddx3xb in zebrafish undergoes liquid-liquid phase separation (LLPS) via its N-terminal intrinsically disordered region (IDR), and an increase in ATP content promotes the condensation of Ddx3xb during MZT. Mutant form of Ddx3xb losing LLPS ability fails to rescue the developmental defect of Ddx3xb-deficient embryos. Interestingly, the IDR of either FUS or hnRNPA1 can functionally replace the N-terminal IDR in Ddx3xb. Phase separation of Ddx3xb facilitates the unwinding of 5' UTR structures of maternal mRNAs to enhance their translation. Our study reveals an unprecedent mechanism whereby the Ddx3xb phase separation regulates MZT by promoting maternal mRNA translation.
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http://dx.doi.org/10.1038/s41422-022-00655-5DOI Listing
June 2022

Cullin-associated and neddylation-dissociated 1 protein (CAND1) governs cardiac hypertrophy and heart failure partially through regulating calcineurin degradation.

Pharmacol Res 2022 May 31;182:106284. Epub 2022 May 31.

Department of Pharmacology, Key Laboratory of Cardiovascular Research, Ministry of Education, College of Pharmacy, Harbin Medical University, Harbin 150086, China; Research Unit of Noninfectious Chronic Diseases in Frigid Zone, Chinese Academy of Medical Sciences, 2019RU070, China; NHC Key Laboratory of Cell Transplantation, Harbin Medical University, Harbin, China. Electronic address:

Pathological cardiac hypertrophy is a process characterized by significant disturbance of protein turnover. Cullin-associated and Neddylation-dissociated 1 (CAND1) acts as a coordinator to modulate substrate protein degradation by promoting the formation of specific cullin-based ubiquitin ligase 3 complex in response to substrate accumulation, which thereby facilitate the maintaining of normal protein homeostasis. Accumulation of calcineurin is critical in the pathogenesis of cardiac hypertrophy and heart failure. However, whether CAND1 titrates the degradation of hypertrophy related protein eg. calcineurin and regulates cardiac hypertrophy remains unknown. Therefore, we aim to explore the role of CAND1 in cardiac hypertrophy and heart failure and the underlying molecular mechanism. Here, we found that the protein level of CAND1 was increased in cardiac tissues from heart failure (HF) patients and TAC mice, whereas the mRNA level did not change. CAND1-KO+ /- aggravated TAC-induced cardiac hypertrophic phenotypes; in contrast, CAND1-Tg attenuated the maladaptive cardiac remodeling. At the molecular level, CAND1 overexpression downregulated, whereas CAND1-KO+ /- or knockdown upregulated calcineurin expression at both in vivo and in vitro conditions. Mechanistically, CAND1 overexpression favored the assembly of Cul1/atrogin1/calcineurin complex and rendered the ubiquitination and degradation of calcineurin. Notably, CAND1 deficiency-induced hypertrophic phenotypes were partially rescued by knockdown of calcineurin, and application of exogenous CAND1 prevented TAC-induced cardiac hypertrophy. Taken together, our findings demonstrate that CAND1 exerts a protective effect against cardiac hypertrophy and heart failure partially by inducing the degradation of calcineurin.
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http://dx.doi.org/10.1016/j.phrs.2022.106284DOI Listing
May 2022

Succession of function, assembly, and interaction of microbial community in sequencing biofilm batch reactors under selenite stress.

Environ Res 2022 Sep 2;212(Pt E):113605. Epub 2022 Jun 2.

Key Laboratory of Industrial Ecology and Environmental Engineering (MOE), Dalian POCT Laboratory, School of Environmental Science and Technology, Dalian University of Technology, Dalian, 116024, China.

The mechanism of interaction between selenite, a toxic substance, and the microbial community in wastewater is still not well understood. Herein, nine sequencing biofilm batch reactors were used to systematically investigate the response of the microbial community to the continuous selenite stress. The results showed that selenite affected the reactor performance and reduced the biofilm mass. Also, it increased the proportion of the living cells, and changed the protein and polysaccharide composition of the biofilm as well as cellular secretions. Selenite facilitated the removal of NO-N, according to water-quality and bioinformatics analyses. As such, the selenite was converted into selenium nanoparticles. α-diversity analysis further revealed that 20 μM selenite enhanced the microbial community resilience, while 200 μM selenite had the reverse effect. Community composition analysis showed that Variovorax, Rhizobium, and Simkania had positive correlations with selenite (P < 0.05). Functional prediction suggested that selenite changed the C, N, and S cycle functions. Furthermore, determinism dominated the community assembly process, and the deterministic proportion increased with the increase of selenite concentration. Network analysis showed that selenite improved the stability and positive correlation ratio of the overall microbial network, and accelerated the communication between microorganisms. However, when compared with the 20 μM selenite, the 200 μM selenite boosted the competition and parasitism/predation among microorganisms. Low-abundance genera played a key role in the network of selenite-reducing microbial community. In addition, under selenite stress, biofilm network exhibited better stability and faster information exchange than suspended network, and the positive association between biofilm and suspended microorganisms increased. All in all, this research sheds light on the interaction between selenite and microbial community, as well as provides crucial information on selenium-containing wastewater.
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http://dx.doi.org/10.1016/j.envres.2022.113605DOI Listing
September 2022

A Systematic Review and Single-center Experience of Azygos Vein Defibrillator Lead Insertion.

J Innov Card Rhythm Manag 2022 May 15;13(5):4987-4993. Epub 2022 May 15.

Department of Internal Medicine, University of Iowa Health Care, Carver College of Medicine, Iowa City, IA, USA.

Defibrillation threshold (DFT) testing is performed in individuals with higher predicted risks of defibrillation failure. Many strategies have been explored to overcome the challenge of high DFT, including an insertion of a defibrillator lead into the azygos vein. We performed a systematic review of the literature to evaluate the safety and efficacy of azygos vein implantable cardioverter-defibrillator insertion for high DFT combined with the analysis of a single-center experience of the procedure at our institution. The literature search was performed in PubMed and Embase from database inception to December 2020 to identify all case reports and case series related to azygos vein defibrillator lead insertion. Our search identified 291 records. After excluding duplicate studies and those without DFT thresholds and non-azygos vascular destinations, 12 studies (23 cases) were reviewed from the current database. We also conducted a retrospective analysis of 5 cases performed at our institution, and a total of 28 patients were included in our final analysis. The mean age of the pooled cohort was 47 years (range, 17-88 years). Men composed 92% of the total cases, and the average body mass index was 34 kg/m. The mean ejection fraction (EF) was 25%, with 78% having non-ischemic cardiomyopathy. The left axillary (36%) or subclavian (48%) vein was the common percutaneous access point. The mean duration of azygos vein access and lead delivery was 22 min (range, 13-60 min). The average DFT prior to azygos coil insertion was 35 J (range, 20-45 J). Fifty-seven percent of cases achieved substantial DFT improvement, whereas 18% achieved relative improvement compared to the pre-procedural threshold. No immediate or remote procedure-related complications were reported in 24 cases where data were available. During an average follow-up period of 18 months, 5 patients had ventricular arrhythmic events requiring device therapy and 4 had successful cardioversion from the device. One patient died from cardiac arrest with variable device therapies of both unsuccessful and successful events. In conclusion, azygos vein defibrillator lead insertion has a considerable rate of success, ease of vascular access with minimal procedural time, and lower risks and complications. Larger studies and longer follow-up periods are warranted to establish its efficacy and safety.
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http://dx.doi.org/10.19102/icrm.2022.130502DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9154010PMC
May 2022

Support Vector Machine versus Multiple Logistic Regression for Prediction of Postherpetic Neuralgia in Outpatients with Herpes Zoster.

Pain Physician 2022 05;25(3):E481-E488

Department of Anesthesiology, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

Background: Postherpetic neuralgia (PHN), as the most common complication of herpes zoster (HZ), is very refractory to current therapies. Studies of HZ have indicated that early aggressive pain interventions can effectively prevent PHN; therefore, accurately predicting PHN in outpatients with HZ and treating HZ promptly, would be of great benefit to patients. Multiple logistic regression (MLR) has often been used to predict PHN. However, support vector machine (SVM) has been poorly studied in predicting PHN in outpatients with HZ.

Objective: The aim of our retrospective study was to analyze the data of outpatients with HZ to evaluate the use of SVM for predicting PHN by comparing it with MLR.

Study Design: A retrospective study.

Setting: Department of Anesthesiology in China.

Methods: The data of 732 outpatients with HZ from January 1, 2015 to May 31, 2020 were reviewed. Risk factors for having PHN in outpatients with HZ were screened using least absolute shrinkage and selection operator (LASSO) algorithm. Then, SVM and MLR were used to predict PHN in outpatients with HZ based on screened risk factors. The data from 600 patients were used for training set and another 132 patients for test set. The receiver operating characteristic (ROC) curve was drawn from the 132 test set of patients. The prediction accuracy of the models was assessed using the area under curve (AUC).

Results: The incidence of having PHN in outpatients with HZ was 19.4%. The risk factors selected by LASSO algorithm were gender, age, VAS scores, skin lesion area, initial treatment time, anxiety, sites of HZ (multiple skin lesions), types of HZ (bullous) and types of pain (knife cutting). The AUC for the SVM and MLR in test set were 0.884 versus 0.853. According to the ROC curve, the specificity and the sensitivity were 0.879 and 0.840 for SVM, and 0.780 and 0.840 for MLR, respectively.

Limitations: Retrospective study and relatively small sample size.

Conclusions: Both SVM and MLR had good discriminative power, but SVM has better performance in predicting PHN in outpatients with HZ, regarding the prediction accuracy and specificity.
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May 2022

Integratable photodetectors based on photopolymerized conductive polymer via femtosecond laser direct writing.

Opt Lett 2022 Jun;47(11):2630-2633

Conductive polymers have attracted a great deal of attention due to their remarkable electrical conductivity. However, the low solubility and inability to meet the limit for the flexible patterning fabrication ability of conductive polymers hinders their applications in miniaturized and integrated electronic devices. Here, femtosecond laser direct writing (FsLDW) is employed to achieve the in situ fabrication of polypyrrole (PPy) with flexibility. Notably, high-precision flexible patterning with a minimum feature size of 5.2 µm and spatial control over the polymerization of PPy is achieved. Moreover, PPy microwires are constructed into a photodetector that exhibits a responsivity of 644 A/W at 0.1-V bias under ultraviolet (UV) irradiation. Ultimately, an image sensor is fabricated by integrating multiple photodetectors, demonstrating the application potential of FsLDW technology for developing miniaturized and integrated electronic devices based on conductive polymers.
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http://dx.doi.org/10.1364/OL.461197DOI Listing
June 2022

Dynamic DNA 5-hydroxylmethylcytosine and RNA 5-methycytosine reprogramming during early human development.

Genomics Proteomics Bioinformatics 2022 May 26. Epub 2022 May 26.

Center for Reproductive Medicine, Henan Key Laboratory of Reproduction and Genetics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. Electronic address:

After implantation, complex and highly specialized molecular events render functionally distinct organ formation, whereas how the epigenome shapes organ-specific development remains to be fully elucidated. Here, nano-hmC-Seal, RNA bisulfite sequencing (RNA-BisSeq), and RNA sequencing (RNA-Seq) were performed, and the first multilayer landscapes of DNA 5-hydroxymethylcytosine (5hmC) and RNA 5-methylcytosine (mC) epigenome were obtained in the heart, kidney, liver, and lung of the human foetuses at 13-28 weeks with 123 samples in total. We identified 70,091 and 503 organ-stage-specific differentially hydroxymethylated regions (DhMRs) and mC-modified mRNAs, respectively. The key transcription factors (TFs), T-box transcription factor 20 (TBX20), paired box protein pax-8 (PAX8), krueppel-like factor 1 (KLF1), transcription factor 21 (TCF21), and CCAAT enhancer binding protein beta (CEBPB), specifically contribute to the formation of distinct organs at different stages. Additionally, 5hmC-enriched Alu elements may participate in the regulation of expression of TF-targeted genes. Our integrated studies revealed a putative essential link between DNA modification and RNA methylation, and illustrate the epigenetic maps during human foetal organogenesis, which provide a foundation for understanding the in-depth epigenetic mechanisms for early development and birth defects.
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http://dx.doi.org/10.1016/j.gpb.2022.05.005DOI Listing
May 2022

Genistein accelerates glucose catabolism via activation the GPER-mediated cAMP/PKA-AMPK signaling pathway in broiler chickens.

Life Sci 2022 Aug 28;303:120676. Epub 2022 May 28.

Key Laboratory of Animal Physiology and Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address:

Genistein, the most abundance of phytoestrogens in soybeans, has beneficial effects in regulating metabolism-related disease; however, there is few available literatures about whether genistein regulates glucose metabolism that in turn affects the lipid accumulation in animals or humans. The current study showed that genistein promoted glucose uptake by enhancing glucose transporter-2 (GLUT2) protein level; and it also increased the activity of phosphofructokinase-1 (PFK) and pyruvate dehydrogenase (PDH), and the mRNA level of succinate dehydrogenase (SDH) both in broiler chickens or hepatocytes. Moreover, genistein obviously increased the p-LKB1 and p-AMPKα protein levels both in vivo and in vitro. Furthermore, the enhancement of genistein on glucose uptake and catabolism were reversed in hepatocytes pre-treated with AMPK inhibitor Compound C, and the increasing of genistein on the p-LKB1 and p-AMPKα protein levels were also reversed in hepatocytes pre-treated with PKA inhibitor H89. Importantly, the results showed that genistein simultaneously increased the estrogen receptor β (ERβ) and G protein-coupled estrogen receptor (GPER) protein levels, but the elevation effect of genistein on cAMP content was completely reversed in hepatocytes pre-treated with GPER antagonist G15, rather than ERβ inhibitor PHTPP. Meanwhile, the increasing of p-LKB1 and p-AMPKα protein levels induced by genistein were also reversed in hepatocytes pre-treated with G15. Collectively, our data demonstrated that genistein improves glucose metabolism via activating the GPER-mediated cAMP/PKA-AMPK signaling pathway. These findings provide theoretical basis for genistein as a promising nutritional supplemental to alleviate metabolism disorders and related diseases in animals or even humans.
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http://dx.doi.org/10.1016/j.lfs.2022.120676DOI Listing
August 2022
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