Publications by authors named "Ying Tian"

660 Publications

Amyloid-Related Imaging Abnormalities in 2 Phase 3 Studies Evaluating Aducanumab in Patients With Early Alzheimer Disease.

JAMA Neurol 2021 Nov 22. Epub 2021 Nov 22.

Biogen, Cambridge, Massachusetts.

Importance: The EMERGE and ENGAGE phase 3 randomized clinical trials of aducanumab provide a robust data set to characterize amyloid-related imaging abnormalities (ARIA) that occur with treatment with aducanumab, an amyloid-β (Aβ)-targeting monoclonal antibody, in patients with mild cognitive impairment due to Alzheimer disease or mild Alzheimer disease dementia.

Objective: To describe the radiographic and clinical characteristics of ARIA that occurred in EMERGE and ENGAGE.

Design, Setting, And Participants: Secondary analysis of data from the EMERGE and ENGAGE trials, which were 2 double-blind, placebo-controlled, parallel-group, phase 3 randomized clinical trials that compared low-dose and high-dose aducanumab treatment with placebo among participants at 348 sites across 20 countries. Enrollment occurred from August 2015 to July 2018, and the trials were terminated early (March 21, 2019) based on a futility analysis. The combined studies consisted of a total of 3285 participants with Alzheimer disease who received 1 or more doses of placebo (n = 1087) or aducanumab (n = 2198; 2752 total person-years of exposure) during the placebo-controlled period. Primary data analyses were performed from November 2019 to July 2020, with additional analyses performed through July 2021.

Interventions: Participants were randomly assigned 1:1:1 to high-dose or low-dose intravenous aducanumab or placebo once every 4 weeks. Dose titration was used as a risk-minimization strategy.

Main Outcomes And Measures: Brain magnetic resonance imaging was used to monitor patients for ARIA; associated symptoms were reported as adverse events.

Results: Of 3285 included participants, the mean (SD) age was 70.4 (7.45) years; 1706 participants (52%) were female, 2661 (81%) had mild cognitive impairment due to Alzheimer disease, and 1777 (54%) used symptomatic medications for Alzheimer disease. A total of 764 participants from EMERGE and 709 participants from ENGAGE were categorized as withdrawn before study completion, most often owing to early termination of the study by the sponsor. Unless otherwise specified, all results represent analyses from the 10-mg/kg group. During the placebo-controlled period, 425 of 1029 patients (41.3%) experienced ARIA, with serious cases occurring in 14 patients (1.4%). ARIA-edema (ARIA-E) was the most common adverse event (362 of 1029 [35.2%]), and 263 initial events (72.7%) occurred within the first 8 doses of aducanumab; 94 participants (26.0%) with an event exhibited symptoms. Common associated symptoms among 103 patients with symptomatic ARIA-E or ARIA-H were headache (48 [46.6%]), confusion (15 [14.6%]), dizziness (11 [10.7%]), and nausea (8 [7.8%]). Incidence of ARIA-E was highest in aducanumab-treated participants who were apolipoprotein E ε4 allele carriers. Most events (479 of 488 [98.2%]) among those with ARIA-E resolved radiographically; 404 of 488 (82.8%) resolved within 16 weeks. In the placebo group, 29 of 1076 participants (2.7%) had ARIA-E (apolipoprotein E ε4 carriers: 16 of 742 [2.2%]; noncarriers, 13 of 334 [3.9%]). ARIA-microhemorrhage and ARIA-superficial siderosis occurred in 197 participants (19.1%) and 151 participants (14.7%), respectively.

Conclusions And Relevance: In this integrated safety data set from EMERGE and ENGAGE, the most common adverse event in the 10-mg/kg group was ARIA-E, which occurred in 362 of the 1029 patients (35.2%) in the 10-mg/kg group with at least 1 postbaseline MRI scan, with 94 patients (26.0%) experiencing associated symptoms. The most common associated symptom was headache.

Trial Registrations: ClinicalTrials.gov Identifiers: NCT02484547, NCT02477800.
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http://dx.doi.org/10.1001/jamaneurol.2021.4161DOI Listing
November 2021

Complete Facial Paralysis Caused by Stabbing With a Car Key at the Stylomastoid Foramen.

Ear Nose Throat J 2021 Nov 18:1455613211056548. Epub 2021 Nov 18.

Department of Otolaryngology Head and Neck Surgery, 159407The First Affiliated Hospital of China Medical University, Shenyang, China.

Non-iatrogenic traumatic facial paralysis is most common in intratemporal facial nerve injury caused by temporal bone fracture, followed by intraparotid facial nerve branch injury. Facial paralysis caused by injury to the extratemporal trunk of the facial nerve is extremely rare. We present a case of a 60-year-old man suffering from immediate complete left peripheral facial paralysis due to blunt transection of extratemporal trunk of facial nerve by stabbing with a car key. There was a facial nerve defect about 1 cm in length. The great auricular nerve was grafted to repair the facial nerve. Over 12 months, his facial nerve function improved to a House-Brackmann III/VI.
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http://dx.doi.org/10.1177/01455613211056548DOI Listing
November 2021

Dye-loaded mesoporous polydopamine nanoparticles for multimodal tumor theranostics with enhanced immunogenic cell death.

J Nanobiotechnology 2021 Nov 17;19(1):365. Epub 2021 Nov 17.

Department of Medical Imaging, Jinling Hospital, Medical School of Nanjing University, Nanjing, 210002, People's Republic of China.

Background: Tumor phototherapy especially photodynamic therapy (PDT) or photothermal therapy (PTT), has been considered as an attractive strategy to elicit significant immunogenic cell death (ICD) at an optimal tumor retention of PDT/PTT agents. Heptamethine cyanine dye (IR-780), a promising PDT/PTT agent, which can be used for near-infrared (NIR) fluorescence/photoacoustic (PA) imaging guided tumor phototherapy, however, the strong hydrophobicity, short circulation time, and potential toxicity in vivo hinder its biomedical applications. To address this challenge, we developed mesoporous polydopamine nanoparticles (MPDA) with excellent biocompatibility, PTT efficacy, and PA imaging ability, facilitating an efficient loading and protection of hydrophobic IR-780.

Results: The IR-780 loaded MPDA ([email protected]) exhibited high loading capacity of IR-780 (49.7 wt%), good physiological solubility and stability, and reduced toxicity. In vivo NIR fluorescence and PA imaging revealed high tumor accumulation of [email protected] Furthermore, the combined PDT/PTT of [email protected] could induce ICD, triggered immunotherapeutic response to breast tumor by the activation of cytotoxic T cells, resulting in significant suppression of tumor growth in vivo.

Conclusion: This study demonstrated that the as-developed compact and biocompatible platform could induce combined PDT/PTT and accelerate immune activation via excellent tumor accumulation ability, offering multimodal tumor theranostics with negligible systemic toxicity.
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http://dx.doi.org/10.1186/s12951-021-01109-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8596951PMC
November 2021

A thiocarbonate-caged fluorescent probe for specific visualization of peroxynitrite in living cells and zebrafish.

Analyst 2021 Nov 17. Epub 2021 Nov 17.

College of Chemical and Biological Engineering, Taiyuan University of Science and Technology, Taiyuan 030024, PR China.

Peroxynitrite (ONOO), a highly reactive oxygen species (ROS), is implicated with many physiological and pathological processes including cancer, neurodegenerative diseases and inflammation. In this regard, developing effective tools for highly selective tracking of ONOO is urgently needed. Herein, we constructed a concise and specific fluorescent probe NA-ONOO for sensing ONOO by conjugating an ONOO-specific recognition group ((4-methoxyphenylthio)carbonyl, a thiocarbonate derivative) with a naphthalene fluorophore. The probe, NA-ONOO, was in a dark state because the high electrophilicity of (4-methoxyphenylthio)carbonyl disturbs the intramolecular charge transfer (ICT) in the fluorophore. Upon treatment with ONOO, the fluorescent emission was sharply boosted (quantum yield : 3% to 56.6%) owing to an ONOO triggered release of (4-methoxyphenylthio)carbonyl from NA-ONOO. Optical analyses showed that NA-ONOO presented high selectivity and sensitivity toward ONOO. With good cell permeability and biocompatibility, the NA-ONOO probe was successfully applied to imaging and tracing exogenous and endogenous ONOO in living cells and zebrafish. The probe NA-ONOO presents a new recognition group and a promising method for further investigating ONOO in living systems.
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http://dx.doi.org/10.1039/d1an00971kDOI Listing
November 2021

Emodin-Conjugated PEGylation of FeO Nanoparticles for FI/MRI Dual-Modal Imaging and Therapy in Pancreatic Cancer.

Int J Nanomedicine 2021 6;16:7463-7478. Epub 2021 Nov 6.

Department of Radiology, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province, 210029, People's Republic of China.

Background: Pancreatic cancer (PC) remains a difficult tumor to diagnose and treat. It is often diagnosed as advanced by reason of the anatomical structure of the deep retroperitoneal layer of the pancreas, lack of typical symptoms and effective screening methods to detect this malignancy, resulting in a low survival rate. Emodin (EMO) is an economical natural product with effective treatment and few side effects of cancer treatment. Magnetic nanoparticles (MNPs) can achieve multiplexed imaging and targeted therapy by loading a wide range of functional materials such as fluorescent dyes and therapeutic agents.

Purpose: The purpose of this study was to design and evaluate a multifunctional theranostic nanoplatform for PC diagnosis and treatment.

Methods: In this study, we successfully developed EMO-loaded, Cy7-functionalized, PEG-coated FeO (FeO-PEG-Cy7-EMO). Characteristics including morphology, hydrodynamic size, zeta potentials, stability, and magnetic properties of FeO-PEG-Cy7-EMO were evaluated. Fluorescence imaging (FI)/magnetic resonance imaging (MRI) and therapeutic treatment were examined in vitro and in vivo.

Results: FeO-PEG-Cy7-EMO nanoparticles had a core size of 9.9 ± 1.2 nm, which showed long-time stability and FI/MRI properties. Bio-transmission electron microscopy (bio-TEM) results showed that FeO-PEG-Cy7-EMO nanoparticles were endocytosed into BxPC-3 cells, while few were observed in hTERT-HPNE cells. Prussian blue staining also confirmed that BxPC-3 cells have a stronger phagocytic ability as compared to hTERT-HPNE cells. Additionally, FeO-PEG-Cy7-EMO had a stronger inhibition effect on BxPC-3 cells than FeO-PEG and EMO. The hemolysis experiment proved that FeO-PEG-Cy7-EMO can be used in vivo experiments. In vivo analysis demonstrated that FeO-PEG-Cy7-EMO enabled FI/MRI dual-modal imaging and targeted therapy in pancreatic tumor xenografted mice.

Conclusion: FeO-PEG-Cy7-EMO may serve as a potential theranostic nanoplatform for PC.
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http://dx.doi.org/10.2147/IJN.S335588DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8579871PMC
November 2021

Evaluating the prognosis of oral squamous cell carcinoma patients via L3 skeletal muscle index.

Oral Dis 2021 Nov 12. Epub 2021 Nov 12.

Department of Oral & Maxillofacial Surgery, Southern Medical University NanFang Hospital, Guangzhou, China.

Objectives: This study aimed to construct a formula to predict L3 skeletal muscle cross-sectional area (CSA) from C3 CSA and to select the cutoff values to evaluate the nutritional status in OSCC.

Materials And Methods: A total of 220 OSCC patients in Nanfang Hospital were divided into two groups: the training set (n = 100) and the validation set (n = 120). Patients in the training set were performed the preoperative whole-body positron emission tomography-computed tomography (PET/CT) scans, and patients in the validation set received preoperative head-and-neck computed tomography (CT) scans. C3 CSA and L3 CSA were delineated. The predictive formula was established, and the gender-specific thresholds of malnutrition were obtained by X-tile software in training set. Finally, the formula and cutoff values were validated.

Results: The predictive formula was successfully established. The gender-specific cutoff values for L3 SMI were 55.0 cm /m for men and 36.6 cm /m for women. There were no differences between the overall survival (OS) of patients diagnosed with malnutrition and that of patients who are not malnutrition.

Conclusions: Our studies reveal that the L3 CSA could be calculated by C3 CSA conveniently with our formula in OSCC, which allowed us to assess malnutrition with head-and-neck CT image. However, there is no direct connection found between malnutrition and OS in OSCC. Hence, further studies with a larger sample size may be required.
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http://dx.doi.org/10.1111/odi.14074DOI Listing
November 2021

Heavy metal pollution characteristics and health risk assessment of dust fall related to industrial activities in desert steppes.

PeerJ 2021 3;9:e12430. Epub 2021 Nov 3.

College of Agriculture, Ningxia University, Yinchuan, Ningxia, China.

China's desert steppe is the transition zone between the grasslands in central China and the arid desert. Ecological security in this region has long been a subject of debate, both in the local and academic communities. Heavy metals and other pollutants are readily released during industrial production, combustion, and transportation, aggravating the vulnerability of the desert steppes. To understand the impact of industrial activiteis on the heavy metal content of dust fall in the desert steppe, a total of 37 dust fall samples were collected over 90 days. An inductively-coupled plasma mass spectrometer (NexION 350X) was used to measure the concentration of heavy metals Cu, Cd, Cr, Pb, Mn, Co, and Zn in the dust. Using comprehensive pollution index and multivariate statistical analysis methods, we explored the characteristics and sources of heavy metal pollution. We also quantitatively assessed the carcinogenic risks of heavy metals resulting from dust reduction with the help of health risk assessment models. The heavy metals' comprehensive pollution index values in the study area's dust fall were ranked as follows: Zn > Cd > Pb > Mn > Cu > Co > Cr. Among these, Zn, Cd, and Pb were significant pollution factors in the study area, and were affected by industrial production and transportation. The high pollution index was concentrated in the north of the research industrial park and on both sides of a highway. The seven heavy metals' total non-carcinogenic risk index (HI) values were ranked as follows: Mn > Co > Pb > Zn > Cr > Cu > Cd (only the HI of Mn was greater than one). Excluding Mn, the non-carcinogenic and carcinogenic risk index values of the other six heavy metals were within acceptable ranges. Previous studies have also shown that industrial transportation and production have had a significant impact on the heavy metal content of dust fall in the desert steppe.
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http://dx.doi.org/10.7717/peerj.12430DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8571961PMC
November 2021

Pentacyclic spermidine alkaloids with radioprotective and anti-inflammatory activities from .

Org Biomol Chem 2021 Nov 25;19(45):9844-9848. Epub 2021 Nov 25.

Beijing Institute of Radiation Medicine, Beijing 100850, China.

Two pairs of novel pentacyclic spermidine alkaloid enantiomers, (±)-orychoviolines A and B ((±)-1 and (±)-2), were isolated from the seeds of and represented the first example of a 2-piperidinone-fused hydrodibenzofuran skeleton, constructed from a 6/5/6/6 tetracyclic system and an 18 atomic ring. The most unexpected novelty was the formation of one more piperidinone ring by a connection between C-6 and N-7. Their structures and absolute configurations were determined by spectroscopic analyses, X-ray crystallography, and ECD analysis. Compared to Ex-RAD (sodium salt of 4-carboxystyryl-4-chlorobenzylsulfone), (-)-1 exhibited a significant radioprotective effect on cell survival and DNA damage. (-)-1 also exhibited remarkable anti-inflammatory activity by inhibiting the production of NO in RAW 264.7 cells activated by lipopolysaccharide with an IC value of 20.3 ± 1.58 μM, which was equivalent to that of dexamethasone.
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http://dx.doi.org/10.1039/d1ob01973bDOI Listing
November 2021

Association of maternal exposure to perfluoroalkyl and polyfluroalkyl substances with infant growth from birth to 12 months: A prospective cohort study.

Sci Total Environ 2021 Oct 29:151303. Epub 2021 Oct 29.

Department of Environmental Health, School of Public Health, Shanghai Jiao Tong University School of Medicine, 200025 Shanghai, China; MOE-Shanghai Key Laboratory of Children's Environmental Health, Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 200092 Shanghai, China. Electronic address:

Background: Although maternal perfluoroalkyl and polyfluroalkyl substances (PFASs) were associated with adverse birth outcomes, much less is known about their impact on infant growth during early infancy.

Objectives: We investigated the association between maternal PFASs exposure and infant growth during the first 12 months of life.

Methods: Participating 2395 pregnancies were recruited from Shanghai Birth Cohort between 2013 and 2016. Ten PFASs were quantified from maternal plasma collected during early pregnancy (median, 15 gestational weeks). We measured infant length, weight, and head circumference at birth, 42 days, 6 months, and 12 months. Linear mixed regression model was used to estimate the associations between PFAS concentrations and repeated measurements of infant growth. Effect modification by infant sex was estimated.

Results: Elevated perfluoroheptanoic acid (PFHpA) concentration was negatively associated with infant length-for-age Z score (LAZ) (β = -0.06, 95% confidence interval (CI): -0.11, -0.01) during the first year. Adverse associations were also observed for perfluorobutane sulfonate (PFBS) and weight-for-length Z score (WFL) (β = -0.02, 95% CI: -0.04, -0.00) and BMI-for-age Z score (BAZ) (β = -0.02, 95% CI: -0.04, -0.00). However, perfluorododecanoic acid (PFDoA) was positively associated with WFL (β = 0.03, 95% CI: 0.00, 0.06) and BAZ (β = 0.03, 95% CI: 0.00, 0.06). The adverse association of PFHpA and LAZ was more pronounced among males (β = -0.06; 95% CI: -0.11, -0.00) than females (β = 0.06; 95% CI: 0.01, 0.12).

Conclusions: In our study, negative associations were found for maternal PFHpA exposure and infant LAZ, PFBS and WFL and BAZ. Meanwhile, maternal PFDoA exposure was positively related with WFL and BAZ. The adverse association of maternal PFHpA exposure and infant LAZ was more pronounced among males. The results should be interpreted with caution, further prospective cohort studies with longitudinal and detailed measures are warranted to confirm these findings.
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http://dx.doi.org/10.1016/j.scitotenv.2021.151303DOI Listing
October 2021

Genome-wide DNA methylation profile changes associated with shell colouration in the Yesso scallop (Patinopecten yessoensis) as measured by whole-genome bisulfite sequencing.

BMC Genomics 2021 Oct 14;22(1):740. Epub 2021 Oct 14.

Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian, China.

Background: Mollusca, a phylum of highly rich species, possess vivid shell colours, but the underlying molecular mechanism remains to be elucidated. DNA methylation, one of the most common epigenetic modifications in eukaryotes, is believed to play a vital role in various biological processes. However, analysis of the effects of DNA methylation on shell colouration has rarely been performed in molluscs, limiting the current knowledge of the molecular mechanism of shell colour formation.

Results: In the present study, to reveal the role of epigenetic regulation in shell colouration, WGBS, the "gold standard" of DNA methylation analysis, was first performed on the mantle tissues of Yesso scallops (Patinopecten yessoensis) with different shell colours (brown and white), and DNA methylomes at single-base resolution were generated. About 3% of cytosines were methylated in the genome of the Yesso scallop. A slight increase in CG percentage and methylation level was found in brown scallops. Sequence preference of nearby methylated cytosines differed between high and low methylation level sites and between the brown- and white-shelled scallops. DNA methylation levels varied among the different genomic regions; all the detected regions in the brown group exhibited higher methylation levels than the white group. A total of 41,175 DMRs (differentially methylated regions) were detected between brown and white scallops. GO functions and pathways associated with the biosynthesis of melanin and porphyrins were significantly enriched for DMRs, among which several key shell colour-related genes were identified. Further, different correlations between mRNA expression levels and DNA methylation status were found in these genes, suggesting that DNA methylation regulates shell colouration in the Yesso scallop.

Conclusions: This study provides genome-wide DNA methylation landscapes of Yesso scallops with different shell colours, offering new insights into the epigenetic regulatory mechanism underlying shell colour.
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http://dx.doi.org/10.1186/s12864-021-08055-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8515700PMC
October 2021

Autophagy triggers endoplasmic reticulum stress and C/EBP homologous protein-mediated apoptosis in OGD/R-treated neurons in a caspase-12-independent manner.

J Neurophysiol 2021 Nov 13;126(5):1740-1750. Epub 2021 Oct 13.

Department of Radiology, the Third Medical Centre, Chinese People's Liberation Army General Hospital, Beijing, People's Republic of China.

We reported that a high level of autophagy was initiated by oxygen-glucose deprivation (OGD) and was maintained in neurons even after oxygen-glucose deprivation followed by reoxygenation (OGD/R), accompanied by neuronal apoptosis. This study focused on autophagy-induced apoptosis and its signaling network, especially the role of endoplasmic reticulum stress (ERS). Analysis of primary cultured cortical neurons from mice showed that the autophagy-induced apoptosis depended on caspase-8 and -9 but not on caspase-12. This finding did not mean that the endoplasmic reticulum did not participate in this process. Increases in the levels of endoplasmic reticulum (ER) biomarkers and binding immunoglobulin protein (BiP) were induced by autophagy in OGD/R-treated neurons. In addition, as an apoptotic transcription factor induced by ER stress, C/EBP homologous protein (CHOP) expression was significantly increased in neurons after OGD/R. This result suggested that the autophagy-BiP-CHOP-caspase (8 and 9)-dependent apoptotic signaling pathway at least partly participated in autophagy-induced apoptosis in primary cortical neurons. It revealed that ER induced apoptosis in neurons suffering from OGD/R injury in an ER stress-CHOP-dependent manner rather than a caspase-12-dependent manner. However, more research on signaling or cross-linking networks and intermediate links is needed. The realization of caspase-12-independent BiP-CHOP neuronal apoptosis pathway has expanded our understanding of the neuronal apoptosis network, which may eventually provide endogenous interventional strategies for OGD/R injury after stroke. ER stress induced by autophagy mediates caspase-8- and caspase-9-dependent apoptosis pathways by regulating CHOP in neurons exposed to OGD/R. We hypothesized that the autophagy-BiP-CHOP-caspase (8 and 9)-dependent apoptotic signaling pathway at least partly participated in autophagy-induced apoptosis in primary cortical neurons.
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http://dx.doi.org/10.1152/jn.00649.2020DOI Listing
November 2021

Systematic review and meta-analysis: α-adrenergic receptor blockers in chronic prostatitis.

Ann Palliat Med 2021 Sep;10(9):9870-9878

Department of Urology Surgery, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Background: Prostatitis seriously endangers the health of men. While they have been widely used in recent years, there remains a lack of systematic evaluation of the clinical efficacy of α-receptor blockers (α-RBs)/α-adrenergic receptor blockers (α-ARBs) in its treatment. Based on this, this study was developed to systematically evaluate the clinical effect of α-ARB in the treatment of prostatitis.

Methods: Randomized controlled trials (RCTs) studying α-RBs or α-ARBs, placebos, or other measures to treat prostatitis were searched in Cochrane Library, PubMed, Embase, and CBM databases from establishment to December 2020. The quality of included articles was evaluated using the Cochrane System Review Manual and Jadad tools, and a meta-analysis was performed using Review Manager 5.3 software.

Results: A total of six articles meeting the requirements were found and included 450 patients. Meta-analysis showed that the National Institutes of Health Chronic Prostatitis Symptom Index (NIH-CPSI) score [mean difference (MD) =-1.76, 95% confidence interval (CI): (-3.35 to -0.17), and P=0.03], pain score [MD =-2.24, 95% CI: (-3.65 to -0.83), and P=0.002], voiding symptom score [MD =-1.21, 95% CI: (-2.06 to -0.35), and P=0.006], and quality of life score [MD =-1.40, 95% CI: (-1.48 to -1.33), and P<0.00001] for patients in the experimental group were lower in contrast to those in the control group after the treatment.

Discussion: The use of α-ARB could significantly improve the treatment effect of patients with prostatitis and improve their quality of life.
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http://dx.doi.org/10.21037/apm-21-2160DOI Listing
September 2021

Combined association of early exposure to long-chain n-3 polyunsaturated fatty acids, mercury and selenium with cognitive performance in 1-year-old infants.

Environ Res 2021 Oct 7:112186. Epub 2021 Oct 7.

Department of Obstetrics and Gynecology, Vagelos College of Physicians and Surgeons, Columbia University, New York, USA; Department of Epidemiology, Mailman School of Public Health, Columbia University, New York, USA. Electronic address:

Background: Previous studies on long-chain n-3 polyunsaturated fatty acids (LCn3PUFAs) and infant neurodevelopment did not consider effect modifications of mercury (Hg) and selenium (Se).

Objectives: To examine the joint association of prenatal LCn3PUFAs, Hg and Se with infant cognitive performance, and to explore whether DNA methylation may explain this potential association.

Methods: A total of 484 newborns were enrolled from the Shanghai Birth Cohort with available data on cord blood LCn3PUFA, nail Hg and Se during 2015-2016. Cord blood LCn3PUFA concentrations were assessed by gas chromatography, and nail Hg and Se concentrations were measured using clippings collected within 6 months of birth by inductively coupled plasma mass spectrometry. Five aspects of infant neurodevelopment (communication, gross motor, fine motor, problem-solving, and personal-social skills) were assessed using the Age and Stage Questionnaire (ASQ) at ages 6 and 12 months. Multivariable-adjusted generalized estimating equations models were performed to examine the associations between cord blood LCn3PUFA concentrations and ASQ test scores, and these associations were stratified by nail Hg and Se levels. Epigenome-wide DNA methylation in cord blood was compared in a random subgroup consisting of 19 infants from the highest and 21 from the lowest decile of LCn3PUFA concentrations.

Results: LCn3PUFAs were not significantly associated with any ASQ test scores. However, in the subgroup with lower Hg (
Conclusion: The association of prenatal LCn3PUFA concentrations with infant neurodevelopment, particularly gross motor skills, may be observed among infants with high Se and low Hg levels.
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http://dx.doi.org/10.1016/j.envres.2021.112186DOI Listing
October 2021

Chromosomal-Level Genome Assembly of a True Bug, Aspongopus chinensis Dallas, 1851 (Hemiptera: Dinidoridae).

Genome Biol Evol 2021 Oct;13(10)

Institute of Entomology, Guizhou University, Guiyang, China.

The true bug, Aspongopus chinensis Dallas, 1851 (Hemiptera: Dinidoridae), is a fascinating insect with prolonged diapause and medicinal properties but also a notorious pest. However, because of the lack of genomic resources, an in-depth understanding of its biological characteristics is lacking. Here, we report the first genome assembly of A. chinensis anchored to 10 pseudochromosomes, which was achieved by combining PacBio long reads and Hi-C sequencing data. This chromosome-level genome assembly was 1.55 Gb in size with a scaffold N50 of 156 Mb. The benchmarking universal single-copy ortholog (BUSCO) analysis of the assembly captured 96.6% of the BUSCO genes. A total of 686,888,052 bp of repeat sequences, 18,511 protein-coding genes, and 1,749 noncoding RNAs were annotated. By comparing the A. chinensis genome with that of 8 homologous insects and 2 model organisms, 213 rapidly evolving gene families were identified, including 83 expanded and 130 contracted gene families. The functional enrichment of Gene Ontology and KEGG pathways showed that the significantly expanded gene families were primarily involved in metabolism, immunity, detoxification, and DNA/RNA replication associated with stress responses. The data reported here shed light on the ecological adaptation of A. chinensis and further expanded our understanding of true bug evolution in general.
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http://dx.doi.org/10.1093/gbe/evab232DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8557641PMC
October 2021

Correction: PSC-derived Galectin-1 inducing epithelial-mesenchymal transition of pancreatic ductal adenocarcinoma cells by activating the NF-κB pathway.

Oncotarget 2021 Sep 28;12(20):2111-2113. Epub 2021 Sep 28.

Department of General Surgery, Institute of General Surgery, Northern Jiangsu Province Hospital, Clinical Medical College, Yangzhou University, Yangzhou, P.R. China.

[This corrects the article DOI: 10.18632/oncotarget.21212.].
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http://dx.doi.org/10.18632/oncotarget.28087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8487725PMC
September 2021

The complete chloroplast genome of (Rosaceae), a medicinal plant found in northwest China.

Mitochondrial DNA B Resour 2021 24;6(10):3057-3058. Epub 2021 Sep 24.

College of Forestry, Gansu Agriculture University, Lanzhou, Gansu, China.

Batalin 1892 is a medicinal plant widely distributed in northwest China. In this study, we report and characterize the complete chloroplast (cp) genome sequence of . The entire sequence is 159,179 bp in length, consisting of the large single-copy region (LSC) and small single copy region (SSC) (87,239 and 19,180 bp, respectively); these two regions are separated by a pair of 26,380-bp inverted repeat (IR) regions. The genome contains 131 genes, including 86 protein-coding genes, 37 tRNA genes, and eight rRNA genes. The overall GC content of the genome is 36.7%. A phylogenetic tree constructed from 18 chloroplast genomes revealed that was clustered with and all of which belong to the genus which is supported as a sister group by a moderate bootstrap support value of 55% with the and genera.
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http://dx.doi.org/10.1080/23802359.2021.1978892DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8475127PMC
September 2021

The Infection and Pathogenicity of SARS-CoV-2 Variant B.1.351 in hACE2 Mice.

Virol Sin 2021 Sep 27. Epub 2021 Sep 27.

State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMS, Beijing, 100071, China.

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http://dx.doi.org/10.1007/s12250-021-00452-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8475296PMC
September 2021

Circ_PIP5K1A regulates cisplatin resistance and malignant progression in non-small cell lung cancer cells and xenograft murine model via depending on miR-493-5p/ROCK1 axis.

Respir Res 2021 Sep 18;22(1):248. Epub 2021 Sep 18.

The Public Health, Xi'an Jiaotong University Health Science Center, No.76 Yanta West Road, Xi'an, 710061, Shaanxi Province, China.

Background: Chemoresistance limits the therapeutic effect of cisplatin (DDP) on non-small cell lung cancer (NSCLC). Circular RNAs (circRNAs) function as important regulators in chemoresistance. This study aimed to explore the regulation of circRNA Phosphatidylinositol-4-Phosphate 5-Kinase Type 1 Alpha (circ_PIP5K1A) in DDP resistance.

Methods: The expression analysis of circ_PIP5K1A, micoRNA-493-5p (miR-493-5p) and Rho Associated Coiled-Coil Containing Protein Kinase 1 (ROCK1) was conducted through reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Cell sensitivity was determined using 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyl tetrazolium bromide (MTT) assay. Cell proliferation and cell viability were evaluated by colony formation assay and MTT assay, respectively. Cell cycle and apoptosis detection was performed via flow cytometry. Cell motility was examined by transwell migration or invasion assay. Dual-luciferase reporter assay was applied to confirm the target binding. ROCK1 protein level was assayed via Western blot. In vivo assay was carried out using xenograft model in mice.

Results: Circ_PIP5K1A level was abnormally increased in DDP-resistant NSCLC tissues and cells. Silencing circ_PIP5K1A reduced DDP resistance, proliferation, cell cycle progression and cell motility in DDP-resistant NSCLC cells. Circ_PIP5K1A directly interacted with miR-493-5p in NSCLC cells. The function of circ_PIP5K1A was dependent on the negative regulation of miR-493-5p. MiR-493-5p directly targeted ROCK1 and circ_PIP5K1A regulated the ROCK1 level via acting as a sponge of miR-493-5p. Overexpression of miR-493-5p inhibited chemoresistance and cancer progression by downregulating ROCK1 expression in DDP-resistant NSCLC cells. Circ_PIP5K1A regulated DDP sensitivity in vivo via the miR-493-5p/ROCK1 axis.

Conclusion: These findings suggested that circ_PIP5K1A upregulated the ROCK1 expression to promote DDP resistance and cancer progression in NSCLC by sponging miR-493-5p.
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http://dx.doi.org/10.1186/s12931-021-01840-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8449484PMC
September 2021

Curative Effect of Early Full Nursing Combined with Postdischarge Continuation Nursing on Patients after Craniocerebral Trauma.

Evid Based Complement Alternat Med 2021 4;2021:7424855. Epub 2021 Sep 4.

Department of Nursing, The First Affiliated Hospital of Kunming Medical University, Kunming 650000, Yunnan, China.

Early full nursing helps patients with some dysfunctions speed up the reorganization of central nervous system functions and coordinate muscle and limb activities. Postdischarge continuation nursing for patients who have not fully recovered after being discharged from the hospital can transfer nursing work from the hospital to the family to meet their nursing needs. In this study, early full nursing combined with postdischarge continuation nursing was used for patients with traumatic brain injury to explore its efficacy and its impact on patients' motor function, quality of life, and complications. The results of the study show that the total effective rate of the observation group (95.92%) was higher than that of the control group (85.71%). At discharge and 1 month, 3 months, and 6 months after discharge, the upper limb Fugl-Meyer score, lower limb Fugl-Meyer score, ARAT score, FIM score, 4 dimensions of GQOLI-74 score, and Barthel index scores of the observation group were higher than those of the control group in the same period. The postoperative complication rate (10.20%) of the observation group was lower than that of the control group (26.53%). Early full nursing combined with postdischarge continuation nursing can improve the rehabilitation effect, effectively promote the recovery of motor function in patients with traumatic brain injury, improve the quality of life, and reduce postoperative complications.
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http://dx.doi.org/10.1155/2021/7424855DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8437603PMC
September 2021

Interleukin-6: A Novel Target for Cardio-Cerebrovascular Diseases.

Front Pharmacol 2021 24;12:745061. Epub 2021 Aug 24.

Institute of Clinical Research, Affiliated Nanhua Hospital, Hengyang Medical College, University of South China, Hengyang, China.

Cardio-Cerebrovascular Disease is a collective term for cardiovascular disease and cerebrovascular disease, being a serious threat to human health. A growing number of studies have proved that the content of inflammatory factors or mediators determines the stability of vascular plaque and the incidence of cardio-cerebrovascular event, and involves in the process of Cardio-Cerebrovascular Diseases. Interleukin-6 is a widely used cytokine that causes inflammation and oxidative stress, which would further result in cardiac and cerebral injury. The increased expression of interleukin-6 is closely related to atherosclerosis, myocardial infarction, heart failure and ischemic stroke. It is a key risk factor for these diseases by triggering inflammatory reaction and inducing other molecules release. Therefore, interleukin-6 may become a potential target for Cardio-Cerebrovascular Diseases in the future. This paper is aimed to discuss the expression changes and pathological mechanisms of interleukin-6 in Cardio-Cerebrovascular Diseases, and to provide a novel strategy for the prevention and treatment of Cardio-Cerebrovascular Diseases.
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http://dx.doi.org/10.3389/fphar.2021.745061DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421530PMC
August 2021

Enhancing red luminescence by doping Yb into Er self-sensitized GdOS upconverting nanoparticles under excitation at 1530 nm.

Dalton Trans 2021 Oct 5;50(38):13468-13475. Epub 2021 Oct 5.

School of Science, Dalian Maritime University, Dalian, Liaoning 1160126, P. R. China.

Red upconversion luminescence (UCL) nanoparticles are of significant importance for applications in the fields of deep tissue imaging, photothermal therapy and security ink. In this work, a highly efficient red emission was achieved by introducing Yb ions as mediators in Er self-sensitized GdOS nanoparticles under excitation at 1530 nm. The results show that the GdOS:Yb,Er nanoparticles synthesized by a homogeneous precipitation method exhibit a uniform spherical shape and narrow size distribution with a mean particle diameter of ≈65 nm. Moreover, the integral emission intensity ratio of red to green of the GdOS:Yb,Er sample is significantly enhanced 3-fold compared with the GdOS:Er sample without Yb doping. The enhancement mechanisms are discussed in detail on the basis of steady-state luminescence spectra and decay dynamics measurements under various excitations at 380, 808, 980 and 1530 nm, respectively. It has been demonstrated that the enhanced red luminescence is induced by cross-relaxation energy transfer from Er to YbS (Er) + F (Yb) → I (Er) + F (Yb) and I (Er) + F (Yb) → I (Er) + F (Yb), and further followed by back energy transfer from Yb to Er through I (Er) + F (Yb) → F (Er) + F (Yb). The former cross-relaxation procedure effectively populates the red emission level of F by depopulating the green emission level of S. Our findings provide a feasible way to enhance the red UCL and new insights into red UCL mechanisms in the Er self-sensitized system under ≈1500 nm excitation, by combining with the nontoxic oxysulfide host, indicating their potential application as safe fluorescent nanoprobes in the bio-field.
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http://dx.doi.org/10.1039/d1dt01929eDOI Listing
October 2021

Comprehensive evaluation of soil quality in a desert steppe influenced by industrial activities in northern China.

Sci Rep 2021 09 1;11(1):17493. Epub 2021 Sep 1.

College of Agriculture, Ningxia University, Yinchuan, 750021, Ningxia, China.

Desert steppe soil security issues have been the focus of attention. Therefore, to understand the impact of industrial activities on the soil quality of desert grasslands, this experiment investigated the Gaoshawo Industrial Concentration Zone in Yanchi County. Based on the distance and direction from the industrial park, sample plots were established at intervals of 1-2 km. A total of 82 surface soil samples (0-20 cm) representing different pollution sources were collected. The samples were analysed for pH, total nitrogen, total phosphorus, available phosphorus, available potassium, organic matter, copper (Cu), cadmium (Cd), chromium (Cr), lead (Pb), and zinc (Zn). The desert steppe soil quality was analysed based on the integrated fertility index (IFI) and the Nemerow pollution index (PN), followed by the calculation of the comprehensive soil quality index (SQI), which considers the most suitable soil quality indicators through a geostatistical model. The results showed that the IFI was 0.393, indicating that the soil fertility was relatively poor. Excluding the available potassium, the nugget coefficients of the fertility indicators were less than 25% and showed strong spatial autocorrelation. The average values of Cu, Cd, Cr, Pb and Zn were 21.64 ± 3.26, 0.18 ± 0.02, 44.99 ± 21.23, 87.18 ± 25.84, and 86.63 ± 24.98 mg·kg, respectively; the nugget coefficients of Cr, Pb and Zn were 30.79-47.35%. Pb was the main element causing heavy metal pollution in the study area. Higher PN values were concentrated north of the highway in the study area, resulting in lower soil quality in the northern region and a trend of decreasing soil quality from south to north. The results of this research showed that the average SQI was 0.351 and the soil quality was extremely low. Thus, industrial activities and transportation activities in the Gaoshawo Industrial Zone significantly impact the desert steppe soil quality index.
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http://dx.doi.org/10.1038/s41598-021-96948-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8410822PMC
September 2021

High-Performance Transparent PEDOT: PSS/CNT Films for OLEDs.

Nanomaterials (Basel) 2021 Aug 15;11(8). Epub 2021 Aug 15.

Tianjin Key Laboratory of Advanced Fibers and Energy Storage, State Key Laboratory of Separation Membranes and Membrane Processes, School of Material Science and Engineering, Tiangong University, Tianjin 300387, China.

Improved OLED systems have great potential for next-generation display applications. Carbon nanotubes (CNTs) and the conductive polymers poly (3,4-ethylenedioxythiophene): poly (styrene sulfonate) (PEDOT: PSS) have attracted great interest for advanced applications, such as optoelectronic products. In this paper, the simultaneous enhancement of the conductivity, roughness, and adhesion properties of transparent conductive films with PEDOT: PSS/CNTs is reported. These films prepared by a simple spin-coating process were successfully used to produce high-performance organic light-emitting diodes (OLEDs) with an improved lifetime. Addition of PEDOT: PSS lowered the film sheet resistance and CNTs helped to enhance the stability and maintain the lifetime of the OLEDs. In addition, treatment with methanol and nitric acid changed the morphology of the polymer film, which led to greatly reduced sheet resistance, enhanced substrate adhesion, and reduced film roughness. The best performance of the film (PEDOT: PSS: CNT = 110: 1, W/W) was 100.34 Ω/[email protected] 90.1 T%. High transmittance, low sheet resistance, excellent adhesion, and low roughness (3.11 nm) were achieved synchronously. The fabricated OLED demonstrated a low minimum operating voltage (3 V) and could endure high voltage (20 V), at which its luminance reached 2973 cd/m. Thus, the incorporation of CNTs within PEDOT: PSS electrodes has great potential for the improvement of the performance of OLED devices.
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http://dx.doi.org/10.3390/nano11082067DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8398071PMC
August 2021

In vivo and in vitro antiproliferative and antimetastatic effects of hemolymph of Aspongopus chinensis Dallas on breast cancer cell.

J Tradit Chin Med 2021 Aug;41(4):523-529

Institute of Entomology, Guizhou Provincial Key Laboratory for Agricultural Pest Management in Mountainous Region, Guizhou University, Guiyang 550025, China.

Objective: To investigate the effects of Aspongopus(A.)chinensis hemolymph on the proliferation and metastasis of breast cancer cells.

Methods: The in vitro effects of A. chinensis hemolymph were investigated in murine (4T1) and human (HCC1937) breast cancer cell lines. Cytotoxicity, cell apoptosis, and cell migration were evaluated by using the cell counting kit-8 assay, Hoechst staining, and wound healing experiments, respectively. A syngeneic mouse model was established to evaluate the in vivo effects of the hemolymph extract on tumor growth and metastasis. Mouse body weight, tumor size, blood levels of function-related enzymes, and pathological features of the liver and kidney tissues were evaluated.

Results: The hemolymph of A. chinensis significantly inhibited in vitro tumor cell migration and viability while inducing apoptosis. Furthermore, it inhibited in vivo tumor growth and metastasis with a minimal effect on mouse body weight, and did not induce liver or kidney damage.

Conclusion: Our results suggested that the A.chinensis hemolymph has antitumorigenic properties, suggesting it has potential as a novel therapeutic option for the treatment of metastatic breast cancer.
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http://dx.doi.org/10.19852/j.cnki.jtcm.2021.03.004DOI Listing
August 2021

The Boundary Proportion Differential Control Method of Micro-Deformable Manipulator with Compensator Based on Partial Differential Equation Dynamic Model.

Micromachines (Basel) 2021 Jul 5;12(7). Epub 2021 Jul 5.

School of Mechanical Engineering, Hebei University of Technology, Tianjin 300000, China.

It is challenging to accurately judge the actual end position of the manipulator-regarded as a rigid body-due to the influence of micro-deformation. Its precise and efficient control is a crucial problem. To solve the problem, the Hamilton principle was used to establish the partial differential equation (PDE) dynamic model of the manipulator system based on the infinite dimension of the working environment interference and the manipulator space. Hence, it resolves the common overflow instability problem in the micro-deformable manipulator system modeling. Furthermore, an infinite-dimensional radial basis function neural network compensator suitable for the dynamic model was proposed to compensate for boundary and uncertain external interference. Based on this compensation method, a distributed boundary proportional differential control method was designed to improve control accuracy and speed. The effectiveness of the proposed model and method was verified by theoretical analysis, numerical simulation, and experimental verification. The results show that the proposed method can effectively improve the response speed while ensuring accuracy.
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http://dx.doi.org/10.3390/mi12070799DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8306330PMC
July 2021

Reduction of choroidal neovascularization via cleavable VEGF antibodies conjugated to exosomes derived from regulatory T cells.

Nat Biomed Eng 2021 09 26;5(9):968-982. Epub 2021 Jul 26.

State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, P. R. China.

Choroidal neovascularization induced by age-related macular degeneration and retinal neovascularization induced by diabetic retinopathy-two leading causes of blindness-are often treated using antibodies targeting vascular endothelial growth factor (VEGF). Here we report a strong association between inflammation and high VEGF expression in aqueous humour samples from patients with choroidal or retinal neovascularization, and show that intravitreally injected exosomes derived from regulatory T cells and conjugated with an anti-VEGF antibody via a peptide linker that is cleavable by matrix metalloproteinases markedly suppressed ocular neovascularization in mouse and non-human primate models of choroidal neovascularization. The engineered exosomes, which selectively accumulate in the neovascularization lesions, could be adapted for other combination therapies of therapeutic antibodies and anti-inflammatory cargo.
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http://dx.doi.org/10.1038/s41551-021-00764-3DOI Listing
September 2021

Circular RNA circSETD3 hampers cell growth, migration, and stem cell properties in bladder cancer through sponging miR-641 to upregulate PTEN.

Cell Cycle 2021 Aug 21;20(16):1589-1602. Epub 2021 Jul 21.

Department of Urology Surgery, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, P. R. China.

Bladder cancer (BLCA) is a common malignant urothelial cancer in the world. Although circular RNAs (circRNAs) involve in regulating BLCA progression, the role of a novel circular RNA circSETD3 in regulating BLCA pathogenesis has not been studied. The expression of circSETD3, miR-641, PTEN mRNA in BLCA tissues and cell lines were measured using RT-qPCR. The gain-of-function experiments were performed and to detect the effects of circSETD3 on cell proliferation, migration, EMT, and stemness maintenance. Besides, rescue experiments were performed to demonstrate the regulatory mechanism of circSETD3/miR-641/PTEN in BLCA cell malignant phenotypes . CircSETD3 was remarkably downregulated in the cancerous clinical tissues and cell lines, in contrast with their normal counterparts, and circSETD3 tended to be deficient in BLCA patients with larger tumor size, advanced clinical stages, positive lymph metastasis and worse prognosis. In addition, circular isoforms of circSETD3 were more resistant to RNase R+ and actinomycetes D treatment compared to their linear isoforms, and circSETD3 mainly distributed in the cytoplasm of the BLCA cells. Further gain-of-function experiments showed that circSETD3 acted as a tumor suppressor to suppress BLCA cell proliferation, migration, EMT and stemness, and the underlying mechanisms had also been elucidated. Mechanistically, circSETD3 sponged miR-641 to upregulate PTEN, resulting in the blockage of BLCA progression. Our findings indicated that circSETD3 acted as a vital tumor suppressor in BLCA via regulating the miR-641/PTEN axis.
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http://dx.doi.org/10.1080/15384101.2021.1954758DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8409755PMC
August 2021

Associations of paternal and maternal per- and polyfluoroalkyl substances exposure with cord serum reproductive hormones, placental steroidogenic enzyme and birth weight.

Chemosphere 2021 Dec 12;285:131521. Epub 2021 Jul 12.

Department of Environmental Health, School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China; MOE-Shanghai Key Laboratory of Children's Environmental Health, Xin Hua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200093, China. Electronic address:

Background: Maternal per- and polyfluoroalkyl substances (PFAS) exposure has been associated with placental function and fetal growth measures. However, few studies have simultaneously investigated paternal and maternal exposure effects.

Objectives: We evaluated the associations of paternal or maternal PFAS levels with placental function and fetal growth measures.

Methods: We studied six PFAS measured in matched parental serums collected within 3 days before delivery in a birth cohort from LaiZhouWan, China. Outcomes evaluated include cord serum estradiol (n = 351), testosterone (n = 349), placental P450aromatase (n = 125), and birth weight (n = 369). Multiple linear regression was applied to estimate the associations for these outcomes according to paternal or maternal PFAS level after adjusting for socio-demographic confounders. Co-adjustment analysis of both paternal and maternal PFAS in the same model was performed.

Results: Maternal and paternal PFAS levels were correlated (Spearman's r = 0.23-0.45). Maternal PFAS were associated with increased estradiol (e.g.

, Pfoa: β = 0.03, 95%CI: 0.00, 0.07), testosterone (e.g.

, Pfua: β = 0.14, 95%CI: 0.00, 0.27), and P450aromatase (e.g.

, Pfoa: β = 0.13, 95%CI: 0.04, 0.22). Maternal PFAS were also associated with a lower mean of birth weight but the estimated 95% CI included the null. Paternal PFAS were not associated with any of the outcomes evaluated.

Conclusions: Several maternal PFAS were associated with fetal steroid hormones and placental enzymes. Despite a correlation of PFAS level within the couples, no association was found for paternal PFAS exposure on these outcomes. The findings suggest the intrauterine PFAS exposure effect on fetal endocrine hormones and growth is unlikely to be confounded by exposure sources or familial factors shared within the couples.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131521DOI Listing
December 2021

Advances in Supercritical Carbon Dioxide Extraction of Bioactive Substances from Different Parts of L.

Molecules 2021 Jun 30;26(13). Epub 2021 Jun 30.

Department of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine, Beijing 100850, China.

L. has always been a popular area of research due to its various active ingredients and pharmacological effects. is rich in ginkgo flavonoids, ginkgolides, and ginkgolic acid, with anti-inflammation, antioxidation, neuroprotection, anti-platelet agglutination, hypolipidemic effect, anti-cancer, and anti-radiation properties. There are many methods to extract and separate the active components of ginkgo. Among them, supercritical carbon dioxide fluid extraction (SFE-CO) is known for its green, clean, and environment-friendly properties. In this paper, the pharmacological activities, the active components, and structures of different parts of ginkgo, the extraction methods of its effective ingredients, and the application of the SFE-CO method for the extraction and separation of active ingredients in from leaves, seeds, pollen, and roots were reviewed, in order to make best use of ginkgo resources, and provide support and references for the development of SFE-CO of active components from .
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http://dx.doi.org/10.3390/molecules26134011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8271647PMC
June 2021

Self-assembled small messenger RNA nanospheres for efficient therapeutic apoptin expression and synergistic Gene-Chemotherapy of breast cancer.

J Colloid Interface Sci 2021 Dec 12;603:191-198. Epub 2021 Jun 12.

Department of Medical Imaging, Jinling Hospital, School of Medicine, Nanjing University, 210002 Jiangsu, China. Electronic address:

In this work, small self-assembled messenger RNA nanospheres (mRNA-NSs) were successfully prepared by rolling circle transcription on a constructed apoptin plasmid. The self-assembled mRNA-NSs have a uniform diameter of approximately 65 nm, good dispersity in solution, and efficient therapeutic apoptin expression ability. In addition, the mRNA-NSs have a high loading capacity of 8.2% for the antitumor drug doxorubicin (Dox), which can effectively deliver the loaded Dox into 4 T1 cells. Cellular experiments show that Dox-loaded self-assembled messenger RNA nanospheres ([email protected]) can reduce the viability of 4 T1 breast cancer cells by significantly upregulating Bax protein, thereby inducing the activation of Caspase 3 in 4 T1 cells. In vivo experiments show that [email protected] can effectively increase the necrosis of tumor tissue, reduce the expression of Ki67, and exhibit a synergistic gene-chemotherapy effect in breast cancer-bearing mice. Taken together, this study successfully prepared self-assembled apoptin messenger RNA nanospheres (mRNA-NSs), which can improve the expression of the therapeutic protein apoptin and exhibit excellent synergistic antitumor effects after loading Dox, providing new ideas for the gene treatment and chemotherapy of breast cancer.
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http://dx.doi.org/10.1016/j.jcis.2021.06.061DOI Listing
December 2021
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