Publications by authors named "Ying Sun"

2,276 Publications

  • Page 1 of 1

Biomarker Changes and Molecular Signatures Associated with Takayasu Arteritis Following Treatment with Glucocorticoids and Tofacitinib.

J Inflamm Res 2022 3;15:4395-4407. Epub 2022 Aug 3.

Department of Rheumatology, Zhongshan Hospital, Fudan University, Shanghai, People's Republic of China.

Objective: This study aimed to analyze biomarker changes in patients with TAK following treatment with glucocorticoids (GCs) and tofacitinib (TOF).

Methods: Seventeen patients from a prospective TAK cohort treated with GCs and TOF and 12 healthy individuals were recruited. TAK associated cytokines, chemokines, growth factors, and MMPs were analyzed in these patients before and after GCs and TOF treatment, and healthy controls. Molecular signatures associated with clinical features were evaluated.

Results: Patients' cytokines (PTX3, IL-6, IFN-γ), chemokines (IL-16, CCL22, CCL2), growth factors (VEGF), and MMP9 levels were significantly higher at baseline (all p < 0.05), while patients' FGF-2 levels were significantly lower (p = 0.02). After treatment, IL-10 was significantly increased at 6 months (p=0.007), and inflammatory cytokines such as PTX3, IL-6 demonstrated a downward trend. Patients without vascular occlusion had higher baseline CCL22 levels than patients with it (p = 0.05), which remained persistently higher after treatment. Radar plot analysis demonstrated that PTX3 was closely correlated with disease activity. In addition, patients without imaging improvement had relatively higher baseline levels of CCL22, FGF-2, and PDGF-AB (p = 0.056, p = 0.06 and p = 0.08 respectively) and lower baseline levels of TNFα, ESR, and CRP (p=0.04, p=0.056, p=0.07, respectively) compared with patients without it.

Conclusion: GCs and TOF are effective in decreasing inflammatory molecules but have limited efficacy in regulating multiple other markers involved in TAK. PTX3 is a prominent marker for disease activity, and CCL22 may have a predictive value for vascular progression.
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http://dx.doi.org/10.2147/JIR.S369963DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9357419PMC
August 2022

False-negative and false-positive outcomes of computer aided detection on brain metastasis: secondary analysis of a multicenter, multireader study.

Neuro Oncol 2022 Aug 9. Epub 2022 Aug 9.

State Key Laboratory of Oncology in South China, Collaborative Innovation Center, for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China.

Background: Errors have seldom been evaluated in computer-aided detection on brain metastases. This study aimed to analyze false negatives (FNs) and false positives (FPs) generated by a brain metastasis detection system (BMDS) and by readers.

Methods: A deep learning-based BMDS was developed and prospectively validated in a multicenter, multireader study. Ad hoc secondary analysis was restricted to the prospective participants (148 with 1,066 brain metastases and 152 normal controls). Three trainees and three experienced radiologists read the MRI images without and with the BMDS. The number of FNs and FPs per patient, jackknife alternative free-response receiver operating characteristic figure of merit (FOM), and lesion features associated with FNs were analyzed for the BMDS and readers using binary logistic regression.

Results: The FNs, FPs, and the FOM of the stand-alone BMDS were 0.49, 0.38, and 0.97, respectively. Compared with independent reading, BMDS-assisted reading generated 79% fewer FNs (1.98 vs. 0.42, P <0.001); 41% more FPs (0.17 vs. 0.24, P <0.001) but 125% more FPs for trainees (P <0.001); and higher FOM (0.87 vs. 0.98, P <0.001). Lesions with small size, greater number, irregular shape, lower signal intensity, and located on non-brain surface were associated with FNs for readers. Small, irregular, and necrotic lesions were more frequently found in FNs for BMDS. The FPs mainly resulted from small blood vessels for the BMDS and the readers.

Conclusions: Despite the improvement detection performance, attention should be paid to FPs and small lesions with lower enhancement for radiologists, especially for less-experienced radiologists.
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http://dx.doi.org/10.1093/neuonc/noac192DOI Listing
August 2022

Alanine Aminotransferase and Bilirubin Dynamic Evolution Pattern as a Novel Model for the Prediction of Acute Liver Failure in Drug-Induced Liver Injury.

Front Pharmacol 2022 22;13:934467. Epub 2022 Jul 22.

Liver Research Center, Beijing Friendship Hospital, Capital Medical University, National Clinical Research Center for Digestive Diseases, Beijing, China.

To develop, optimize, and validate a novel model using alanine aminotransferase (ALT) and total bilirubin (TB) dynamic evolution patterns in predicting acute liver failure (ALF) in drug-induced liver injury (DILI) patients. The demographics, clinical data, liver biopsy, and outcomes of DILI patients were collected from two hospitals. According to the dynamic evolution of ALT and TB after DILI onset, the enrolled patients were divided into ALT-mono-peak, TB-mono-peak, double-overlap-peak, and double-separate-peak (DSP) patterns and compared. Logistic regression was used to develop this predictive model in both discovery and validation cohorts. The proportion of ALF was significantly higher in patients with the DSP pattern than in the ALT-mono-peak pattern and DOP pattern (10.0 vs. 0.0% vs. 1.8%, < 0.05). The area under receiver operating characteristic curve (AUROC) of the DSP pattern model was 0.720 (95% CI: 0.682-0.756) in the discovery cohort and 0.828 (95% CI: 0.788-0.864) in the validation cohort in predicting ALF, being further improved by combining with international normalized ratio (INR) and alkaline phosphatase (ALP) (AUROC in the discovery cohort: 0.899; validation cohort: 0.958). Histopathologically, patients with the DSP pattern exhibited a predominantly cholestatic hepatitis pattern (75.0%, < 0.05) with a higher degree of necrosis (29.2%, = 0.084). DILI patients with the DSP pattern are more likely to progress to ALF. The predictive potency of the model for ALF can be improved by incorporating INR and ALP. This novel model allows for better identification of high-risk DILI patients, enabling timely measures to be instituted for better outcome.
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http://dx.doi.org/10.3389/fphar.2022.934467DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355525PMC
July 2022

Multifunctional probe based on modified Ag&Mn:ZnInS QDs for dual-mode fluorescence and magnetic resonance imaging of intracellular glutathione.

Anal Chim Acta 2022 Aug 16;1221:340172. Epub 2022 Jul 16.

College of Chemistry, Jilin Province Research Center for Engineering and Technology of Spectral Analytical Instruments, Jilin University, Qianjin Street 2699, Changchun, 130012, China. Electronic address:

Glutathione (GSH) plays vital roles in a variety of biological processes, and the development of simple and effective GSH detection method is an important research topic. Herein, a multifunctional probe based on Ag&Mn:ZnInS quantum dots (QDs) was developed for bimodal imaging of GSH. MnO, as an efficient fluorescence quencher, was in-situ grown on the surface of QDs, and then modified with hyaluronic acid (HA) to improve the stability and targeted recognition capability of the probe due to the binding between HA and CD44 receptors. After MnO was deconstructed by GSH, the fluorescence of the probe was recovered and the generated Mn could serve as good magnetic resonance imaging (MRI) contrast agent. Moreover, the near-infrared emission probe was successfully employed in living cell and zebrafish imaging due to its low toxicity and high anti-biological interference performance. This strategy provides a simple dual-mode fluorescence/MRI imaging of GSH, which may have a broad application in biological detection.
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http://dx.doi.org/10.1016/j.aca.2022.340172DOI Listing
August 2022

Low-dose metronomic chemotherapy improves tumor control in nasopharyngeal carcinoma.

Cancer Commun (Lond) 2022 Aug 4. Epub 2022 Aug 4.

State Key Laboratory of Oncology in South China, Collaborative Innovation Center of Cancer Medicine, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Center for Precision Medicine of Sun Yat-sen University, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong, 510060, P. R. China.

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http://dx.doi.org/10.1002/cac2.12347DOI Listing
August 2022

Computational models, databases and tools for antibiotic combinations.

Brief Bioinform 2022 Aug 1. Epub 2022 Aug 1.

Department of Respiratory Medicine, the First Hospital of Jilin University, Changchun, China.

Antibiotic combination is a promising strategy to extend the lifetime of antibiotics and thereby combat antimicrobial resistance. However, screening for new antibiotic combinations is both time-consuming and labor-intensive. In recent years, an increasing number of researchers have used computational models to predict effective antibiotic combinations. In this review, we summarized existing computational models for antibiotic combinations and discussed the limitations and challenges of these models in detail. In addition, we also collected and summarized available data resources and tools for antibiotic combinations. This study aims to help computational biologists design more accurate and interpretable computational models.
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http://dx.doi.org/10.1093/bib/bbac309DOI Listing
August 2022

Packed-Fiber Solid Phase-Extraction Coupled with HPLC-MS/MS for Rapid Determination of Lipid Oxidative Damage Biomarker 8-Iso-Prostaglandin F in Urine.

Molecules 2022 Jul 10;27(14). Epub 2022 Jul 10.

Key Laboratory of Child Development and Learning Science (Ministry of Education), School of Biological Science & Medical Engineering, Southeast University, Nanjing 210096, China.

The 8-iso-prostaglandin F (8-iso-PGF) biomarker is used as the gold standard for tracing lipid oxidative stress in vivo. The analysis of urinary 8-iso-PGF is challenging when dealing with trace amounts of 8-iso-PGF and the complexity of urine matrixes. A packed-fiber solid-phase extraction (PFSPE)-coupled with HPLC-MS/MS-method, based on polystyrene (PS)-electrospun nanofibers, was developed for the specific determination of 8-iso-PGF in urine and compared with other newly developed LC-MS/MS methods. The method, which simultaneously processed 12 samples within 5 min on a self-made semi-automatic array solid-phase extraction processor, was the first to introduce PS-electrospun nanofibers as an adsorbent for the extraction of 8-iso-PGF and was successfully applied to real urine samples. After optimizing the PFSPE conditions, good linearity in the range of 0.05-5 ng/mL with R > 0.9996 and a satisfactory limit of detection of 0.015 ng/mL were obtained, with good intraday and interday precision (RSD < 10%) and recoveries of 95.3-103.8%. This feasible method is expected to be used for the batch quantitative analysis of urinary 8-iso-PGF.
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http://dx.doi.org/10.3390/molecules27144417DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318247PMC
July 2022

Tax Incentives, Tax Enforcement, and Enterprise R&D Investment: Evidence From Chinese A-Share Listed.

Authors:
Ying Sun

Front Psychol 2022 8;13:953313. Epub 2022 Jul 8.

Department of Economics and Management, Shanghai Administration Institute, Shanghai, China.

This study investigates the relationship between tax incentives, tax enforcement, and R&D investment in Chinese enterprises. Tax compliance is an important part of organizational behavioral psychology, which will impact organizational innovation activities. Therefore, the present study utilizes the panel data of Chinese A-share listed companies collected from the China Stock Market & Accounting Research (CSMAR) database from 2011 to 2020 to conduct empirical research. It tests the policy effect of tax incentives on enterprise R&D investment and examines the impact of tax enforcement on enterprise R&D behavior and its moderating effect on the relationship between tax incentives and enterprise R&D investment. The results show that China's preferential tax policies positively affect enterprise innovation activities, and stable and continuous tax incentives can stimulate enterprises to increase R&D investment. Increasing the intensity of tax enforcement has a significant positive impact on enterprise R&D investment, which means that the promotion effect of "governance effect" and "incentive effect" caused by tax enforcement may exceed the negative impact brought by its "taxation effect" and "rent-seeking effect." The study suggests that with the strengthening of tax enforcement, the promotion effect of tax incentives is weakened, which is only reflected in non-state-owned enterprises. Finally, we propose relevant policy recommendations based on the research results. This includes further optimizing the collocation of tax types and preferential methods, encouraging enterprises to face up to the role of tax enforcement as an external governance mechanism, and promoting the tax department to standardize the enforcement process and taxation services. The paper presents a range of theoretical and practical implications for both firm managers and policymakers.
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http://dx.doi.org/10.3389/fpsyg.2022.953313DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304986PMC
July 2022

Evaluation of the effect of perioperative administration of S(+)-ketamine hydrochloride injection for postoperative acute pain in children: study protocol for a prospective, multicenter, randomized, open-label, parallel-group, pragmatic clinical trial.

Trials 2022 Jul 23;23(1):586. Epub 2022 Jul 23.

Department of Biostatistics, School of Public Health, Southern Medical University, Guangzhou, 510515, People's Republic of China.

Background: Inadequate postoperative pain management increases the risk of adverse events after the surgery and aggressive perioperative pain prevention has both short-term and long-term benefits. S(+)-ketamine is an N-methyl-D-aspartic acid (NMDA) receptor antagonist with a strong analgesic effect and can significantly relieve postoperative acute pain and reduce opioid consumption. However, for children, it still needs to be confirmed by large sample clinical studies.

Methods: This is a pragmatic, randomized controlled trial which will evaluate the effect of perioperative administration of S(+)-ketamine hydrochloride injection for postoperative acute pain in children in a pragmatic clinical setting. A total of 3000 children (≤17 years old) undergoing surgery will be included in this protocol. Subjects will be randomized 2:1 to either receive S(+)-ketamine hydrochloride injection or conventional therapy without S(+)-ketamine during the entire perioperative period. The primary endpoints are the area under the receiver operating characteristic (ROC) curve of Face Legs Activity Cry and Consolability (FLACC, 0-7 years old) scale score or Numerical Rating Scale (NRS, 8-17 years old) score within 48 h after surgery, and the consumption of opioids within 48 h after surgery. The secondary endpoints include the time of first use of rescue analgesics after surgery, rescue analgesia rate within 48 h after surgery, anesthesia recovery time, incidence of emergency delirium (for 0-7 years old), changes of anxiety and depression scale scores at 48 h after surgery (for 8-17 years old), incidence of intraoperative adverse events (AEs), and incidence of postoperative AEs and pharmacoeconomic indicators. AEs and serious AEs were recorded to evaluate safety.

Discussion: This trial will be the first pragmatic clinical trial to prospectively assess the effect of perioperative administration of S(+)-ketamine hydrochloride injection for postoperative acute pain in children, which is of great significance to the continuous optimization of clinical anesthesia and analgesia programs for children.

Trial Registration: This trial was registered in the U.S. National Institutes of Health ClinicalTrials.gov database ( http://clinicaltrials.gov ; Registration number: NCT04834427). Registered on 8 April 2021.
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http://dx.doi.org/10.1186/s13063-022-06534-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9308221PMC
July 2022

Spatial distribution, source apportionment and health risk assessment of inorganic pollutants of surface water and groundwater in the southern margin of Junggar Basin, Xinjiang, China.

J Environ Manage 2022 Jul 18;319:115757. Epub 2022 Jul 18.

College of Water Conservancy and Civil Engineering, Xinjiang Agricultural University, Urumqi, 830052, China; Xinjiang Hydrology and Water Resources Engineering Research Center, Urumqi, 830052, China; Xinjiang Key Laboratory of Hydraulic Engineering Security and Water Disasters Prevention, Urumqi, 830052, China. Electronic address:

Surface water (SW) and groundwater (GW) are crucial water supply sources in the southern margin of Junggar Basin in Xinjiang. The sources of toxic components in SW and GW and their negative effects on human health are of great concern. A total of 40 SW and 596 GW samples were collected at the oasis belt to analyze distribution, sources and potential health risks of inorganic pollutants in SW and GW. Results revealed that SW quality was severely affected by Hg, 30.0% of which had Hg concentration greater than the national drinking water standard. High Hg SW was mainly distributed near Manas County and Urumqi City. GW quality was mostly affected by SO, 24.7% of which had SO concentration greater than the national drinking water standard. High SO GW primarily occurred in the northwest and middle of the study area. Source apportionment of inorganic pollutants identified geological background, municipal wastewater disposal, water-rock interaction, geological environment, geological structure and industrial emission were the prominent potential sources of inorganic pollution in SW, with contribution rates of 1.2%, 10.0%, 43.6%, 35.1%, 6.3% and 3.8%, respectively. Five potential pollution sources in GW (including geological background, municipal wastewater disposal, water-rock interaction, geological environment and aquifer burial depth) were identified, with contribution rates of 0.7%, 9.6%, 77.6%, 11.1% and 1.0%, respectively. Probabilistic health risk assessment showed that Cl and As in SW and GW were the main inorganic pollutants threatening human health. Non-carcinogenic risks for adults and children were negligible, while carcinogenic risks cannot be negligible. Furthermore, the contribution of potential pollution sources to health risks was quantified using positive matrix factorization coupling with health risk assessment model. Based on which, we offered the suggestion that water quality improvement in contaminated areas should be implemented in combination with pollution monitoring systems.
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http://dx.doi.org/10.1016/j.jenvman.2022.115757DOI Listing
July 2022

The Correlation Between Biofilm-Forming Ability of Community-Acquired Methicillin-Resistant Isolated from the Respiratory Tract and Clinical Characteristics in Children.

Infect Drug Resist 2022 12;15:3657-3668. Epub 2022 Jul 12.

Department of Pulmonology, Children's Hospital, Zhejiang University School of Medicine, Hangzhou, 310052, People's Republic of China.

Objective: This study aimed to investigate the biofilm-forming ability, molecular typing, and antimicrobial resistance of community-acquired methicillin-resistant (CA-MRSA) strains isolated from the respiratory tract of children and their correlation with clinical characteristics.

Methods: All CA-MRSA strains were isolated from hospitalized children, and their presentation, molecular typing, antimicrobial susceptibility, and biofilm formation were investigated. The clinical characteristics were compared between the strong and weak biofilm producer groups.

Results: Fifty-three CA-MRSA strains were isolated from the respiratory samples of 53 children, with nearly half of them being young infants (0-12 months). Approximately, 88.7% (47/53) of the isolates were resistant to four or more antibiotics, mainly β-lactam antibiotics, lincosamides, and macrolides. Twelve sequence types (STs) and 20 subtypes of staphylococcal protein A (spa) typing were identified, with ST59-t437 (39.6%, 21/53) as the predominant subtype. All strains showed the ability to form biofilms. When compared to children with weak biofilm-forming CA-MRSA strains, those with strong biofilm-forming strains had higher proportions of lower respiratory tract infections (LRTI) (88.5% vs 59.3%), obvious cough symptoms (84.6% vs 51.9%), and severe chest imaging manifestations (76.9% vs 37.0%). Furthermore, a strong biofilm-forming ability significantly increased the risk of prolonged cough in children with LRTI (44.4% vs 14.3%), and a positive correlation between the duration of cough and the extent of biofilm formation was observed. Medical history investigation revealed that the strong biofilm-forming group had a much higher percentage of macrolides intake than the weak biofilm-forming group in the last month before admission (61.5% vs 14.8%).

Conclusion: ST59-t437 was the most prevalent clone in CA-MRSA respiratory isolates among the hospitalized children. All CA-MRSA strains formed biofilms. The stronger the biofilm-forming ability, the more serious and prolonged were the respiratory symptoms.
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http://dx.doi.org/10.2147/IDR.S370755DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9288189PMC
July 2022

Dendrobium officinale polysaccharide attenuates cognitive impairment in circadian rhythm disruption mice model by modulating gut microbiota.

Int J Biol Macromol 2022 Jul 16;217:677-688. Epub 2022 Jul 16.

Department of Food Science and Engineering, Ningbo University, Ningbo 315211, PR China. Electronic address:

Dendrobium officinale polysaccharide (DOP) has received an increasing amount of attention as it could alleviate AD-related cognitive impairment via the regulation of microglial activation. However, the modulatory mechanism of DOP on circadian rhythm disruption (CRD) and related cognitive impairment needs further investigation. In our study, the circadian rhythm disruption mice showed a deficit in recognition and spatial memory. DOP treatment reshaped the perturbation of gut microbiota caused by CRD, including up-regulated the abundance of Akkermansia and Alistipes, down-regulated the abundance of Clostridia. In addition, DOP restored histopathological changes, reduced inflammatory cells infiltration and strengthened mucosal integrity. Mechanistically, DOP ameliorated intestinal barrier dysfunction by up-regulating tight junction protein expression, which in turn improved the invasion of lipopolysaccharide to blood and brain. The change of these contributes to inhibiting the NF-κB activation and neuroinflammation, and thus attenuating hippocampus neuronal damage and the deposition of Aβ. Meanwhile, our results revealed that DOP could reverse the levels of metabolites derived related to cognitive function improvement, and these metabolites were closely associated with the key microbiota. Therefore, we speculated that DOP has the potential to provide neuroprotection against cognitive impairment by modulating the gut microbiota.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.07.090DOI Listing
July 2022

Two case reports of non-small cell lung cancer patients harboring acquired T790M--C797S benefit from immune checkpoint inhibitor combined with platinum-based doublet chemotherapy.

Ann Transl Med 2022 Jun;10(12):719

Department of Thoracic surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China.

Background: Acquired resistance is inevitable in non-small cell lung cancer (NSCLC) patients treated with epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs). The emergence of exon 20 C797S is one of the major resistance mechanisms to osimertinib as a third-generation EGFR-TKI. To date, there is no standard of care for NSCLC patients after acquiring C797S. Immune checkpoint inhibitors (ICIs) have revolutionized the treatment of various types of cancers in the last decade. Whether NSCLC patients with acquired C797S could benefit from ICIs remains elusive.

Case Description: Herein, we reported two cases of -mutant NSCLC patients who acquired a tertiary mutation C797S benefited from ICIs. A 28-year-old woman presented with anepithymia and nausea. Chest computed tomography (CT) revealed a mass in the right lung. She was diagnosed with stage IV lung adenocarcinoma (LUAD) with exon 19 deletion (19del) based on imaging and next-generation sequencing (NGS) findings. She received icotinib followed by osimertinib, then acquired T790M--C797S. She had low tumor mutation burden (TMB) and achieved partial response (PR) to a programmed cell death-1 (PD-1) inhibitor sintilimab combined with platinum-based doublet chemotherapy as late-line treatment lasting more than 5 months. A 66-year-old man complained with chest tightness, hemoptysis, and back pain. CT scans revealed a mass in the right lung and metastases to the bilateral lungs, liver, adrenal gland, mediastinal lymph nodes, and bone. He was also diagnosed with 19del-positive LUAD and treated with icotinib followed by osimertinib. He also acquired T790M--C797S. The patient had low TMB also and benefited from a PD-1 inhibitor camrelizumab combined with platinum-based doublet chemotherapy as late-line treatment with a progression-free survival (PFS) of 8 months. Two cases had no treatment-related adverse events leading to discontinuation of PD-1 inhibitors.

Conclusions: Our study provides the first clinical evidence that ICIs combined with platinum-based doublet chemotherapy may be effective treatment options for overcoming resistance mediated by T790M--C797S. Clinical trials are needed to evaluate the efficacy and safety of PD-1 inhibitors in the treatment of NSCLC patients harboring T790M--C797S.
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http://dx.doi.org/10.21037/atm-22-2436DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279785PMC
June 2022

Path Planning Optimization of Intelligent Vehicle Based on Improved Genetic and Ant Colony Hybrid Algorithm.

Front Bioeng Biotechnol 2022 1;10:905983. Epub 2022 Jul 1.

Hubei Key Laboratory of Hydroelectric Machinery Design and Maintenance, Three Gorges University, Yichang, China.

Intelligent vehicles were widely used in logistics handling, agriculture, medical service, industrial production, and other industries, but they were often not smooth enough in planning the path, and the number of turns was large, resulting in high energy consumption. Aiming at the unsmooth path planning problem of four-wheel intelligent vehicle path planning algorithm, this article proposed an improved genetic and ant colony hybrid algorithm, and the physical model of intelligent vehicle was established. This article first improved ant colony optimization algorithm about heuristic function with the adaptive change of evaporation factor. Then, it improved the genetic algorithm on fitness function, adaptive adjustment of crossover factor, and mutation factor. Last, this article proposed the improved hybrid algorithm with the addition of a deletion operator, adoption of an elite retention strategy, and addition of suboptimal solutions obtained from the improved ant colony algorithm to improved genetic algorithm to obtain optimized new populations. The simulation environment for this article is windows 10, the processor is Intel Core i5-5257U, the running memory is 4GB, the compilation environment is MATLAB2018b, the number of ant samples is 50, the maximum number of iterations is 100, the initial population size of the genetic algorithm is 200, and the maximum number of iterations is 50. Simulation and physical experiments show that the improved hybrid algorithm is effective. Compared with the traditional hybrid algorithm, the improved hybrid algorithm reduced by 46% in the average number of iterations and 75% in the average number of turns in a simple grid. The improved hybrid algorithm reduced by 47% in the average number of iterations and 21% in the average number of turns in a complex grid. The improved hybrid algorithm works better to reduce the number of turns in simple maps.
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http://dx.doi.org/10.3389/fbioe.2022.905983DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283690PMC
July 2022

Inhibition of mPGES-2 ameliorates NASH by activating NR1D1 via heme.

Hepatology 2022 Jul 15. Epub 2022 Jul 15.

Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu, P. R. China.

Background And Aims: Nonalcoholic fatty liver disease (NAFLD), a complex metabolic syndrome, has limited therapeutic options. Microsomal prostaglandin E synthase-2 (mPGES-2) was originally discovered as a prostaglandin E (PGE ) synthase; however, it does not produce PGE in the liver. Moreover, the role of mPGES-2 in NAFLD remains undefined. Herein, we aimed to determine the function and mechanism of mPGES-2 in liver steatosis and steatohepatitis.

Approach And Results: To evaluate the role of mPGES-2 in NAFLD, whole-body or hepatocyte-specific mPGES-2-deficient mice fed a high-fat or methionine-choline-deficient diet were used. Compared with control mice, mPGES-2-deficient mice showed reduced hepatic lipid accumulation, along with ameliorated liver injury, inflammation, and fibrosis. Furthermore, the protective effect of mPGES-2 deficiency against NAFLD was dependent on decreased cytochrome P450 4A14 and increased acyl-CoA thioesterase 4 levels regulated by the heme receptor nuclear receptor subfamily 1 group D member 1 (NR1D1), but not PGE . Heme regulated the increased NR1D1 activity mediated by mPGES-2 deficiency. Further, we confirmed the protective role of the mPGES-2 inhibitor SZ0232 in NAFLD therapy.

Conclusion: Our study indicates the pathogenic role of mPGES-2 and outlines the mechanism in mediating NAFLD, thereby highlighting the therapeutic potential of mPGES-2 inhibition in liver steatosis and steatohepatitis.
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http://dx.doi.org/10.1002/hep.32671DOI Listing
July 2022

MicroRNA-21-5p promotes mucosal type 2 inflammation via regulating GLP1R/IL-33 signaling in chronic rhinosinusitis with nasal polyps.

J Allergy Clin Immunol 2022 Jul 11. Epub 2022 Jul 11.

Department of Otolaryngology, Head and Neck Surgery, Beijing TongRen Hospital, Capital Medical University, Beijing 100730, China; Beijing Laboratory of Allergic Diseases and Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otolaryngology, Beijing 100005, China; Department of Allergy, Beijing TongRen Hospital, Capital Medical University, Beijing 100730, China. Electronic address:

Background: It has been known that chronic rhinosinusitis with nasal polyps (CRSwNP) is a type 2 inflammation dominated disease, however, the reasons causing such type of mucosal inflammation in CRSwNP are not well elucidated.

Objective: We sought to investigate the role of microRNA-21-5p (miR-21-5p) in regulating mucosal type 2 inflammation in CRSwNP.

Methods: MiR-21-5p expression was detected in nasal mucosa of patients with CRSwNP. Correlations between miR-21-5p and indicators of type 2 inflammation were further analyzed. MiR-21 knockout (KO) mice were used to explore the role of miR-21-5p in a murine model of eosinophilic (E) CRSwNP. Target gene of miR-21-5p related to type 2 inflammation in CRSwNP was identified.

Results: The up-regulated miR-21-5p in nasal mucosa of CRSwNP patients, compared to control subjects, was expressed higher in ECRSwNP than nonECRSwNP patients. MiR-21-5p expression was positively correlated with mucosal eosinophil infiltrations, and the expression of type 2 inflammatory cytokines. In the CRSwNP mice, miR-21KO significantly attenuated type 2 inflammation, as indicated by eosinophil infiltrations and cytokines/chemokines expression in nasal mucosa and lavage fluid; moreover, genes associated with type 2 inflammation were extensively down-regulated at the transcriptome level in miR-21KO mice. Glucagon-like peptide-1 receptor (GLP1R), which was negatively correlated with miR-21-5p expression in human nasal mucosa, was identified as the target of miR-21-5p. Overexpression of miR-21-5p induced IL-33 expression, whereas GLP1R agonist decreased IL-33 production in airway epithelial cells.

Conclusion: MiR-21-5p aggravates type 2 inflammation in nasal mucosa of CRSwNP via targeting GLP1R/IL-33 signaling, which may be a potential therapeutic target for CRSwNP.
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http://dx.doi.org/10.1016/j.jaci.2022.05.030DOI Listing
July 2022

Association of Intratumoral Microbiota With Prognosis in Patients With Nasopharyngeal Carcinoma From 2 Hospitals in China.

JAMA Oncol 2022 Jul 14. Epub 2022 Jul 14.

Department of Experimental Research, State Key Laboratory of Oncology in South China, Sun Yat-sen University Cancer Center, Guangzhou, People's Republic of China.

Importance: Microbiota-tumor interactions have qualified microbiota as a promising prognostic biomarker in various types of cancers. Although the nasopharynx acts as a crucial niche of the upper respiratory tract microbiome, whether the intratumoral microbiota exists and its clinical significance in nasopharyngeal carcinoma (NPC) remain uncertain.

Objective: To evaluate the clinical significance of intratumoral microbiota for individual prognostication in patients with NPC.

Design, Setting, And Participants: This retrospective cohort study included NPC biopsy samples from 2 hospitals: Sun Yat-sen University Cancer Center (Guangzhou, China) and Zhejiang Cancer Hospital (Hangzhou, China) between January 2004 and November 2016, with follow-up through November 2020. A total of 802 patients were included according to the following criteria: with histologically proven NPC, without distant metastasis at initial diagnosis, had not received antitumor treatment before biopsy sampling, aged between 18 and 70 years, with complete medical records and regular follow-up, without a history of cancer, and successfully extracted enough DNA for experiments.

Main Outcomes And Measures: The primary end point was disease-free survival, and the secondary end points included distant metastasis-free survival and overall survival. To assess the existence and load of intratumoral microbiota in 96 patients with NPC with or without tumor relapse, 16S rRNA sequencing and quantitative polymerase chain reaction were used. The associations between intratumoral bacterial load and clinical outcome were evaluated in 241 fresh-frozen NPC samples (training cohort) and validated in paraffin-embedded NPC samples of internal (n = 233) and external (n = 232) validation cohorts. Metagenomic and transcriptome analyses were performed to ascertain the origin and underlying mechanism of intratumoral bacteria.

Results: A total of 802 patients with NPC (mean [SD] age, 46.2 [10.6] years; 594 [74.1%] male) were enrolled. Microbiota presented within NPC tumor tissues, among which Corynebacterium and Staphylococcus predominated. Patients with a high bacterial load in the training cohort had inferior rates of disease-free survival (hazard ratio [HR], 2.90; 95% CI, 1.72-4.90; P < .001), distant metastasis-free survival (HR, 3.18; 95% CI, 1.58-6.39; P < .001), and overall survival (HR, 3.41; 95% CI, 1.90-6.11, P < .001) than those with a low bacterial load, a finding that was validated by the internal and external validation cohorts. Single-nucleotide variant analysis revealed that the nasopharyngeal microbiota was the main origin of NPC intratumoral bacteria. Transcriptome and digital pathology analyses demonstrated that a higher intratumoral bacterial load was negatively associated with T-lymphocyte infiltration.

Conclusions And Relevance: Intratumoral bacterial load was a robust prognostic tool for patients with NPC in this cohort study, indicating potential guidance for treatment decisions in patients at different levels of risk of malignant progression.
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http://dx.doi.org/10.1001/jamaoncol.2022.2810DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9284409PMC
July 2022

TRPC1 correlates with poor tumor features, radiotherapy efficacy and survival in tongue squamous cell carcinoma.

Biomark Med 2022 08 14;16(11):867-877. Epub 2022 Jul 14.

Department of Stomatology, HanDan Central Hospital, Hebei Handan, 056000, China.

The present study aimed to explore the clinical association of TRPC1 with tongue squamous cell carcinoma (TSCC) tumor features and prognosis. A total of 246 TSCC patients who underwent surgical resection were retrospectively analyzed, and their tissue specimens were acquired for TRPC1 protein and mRNA detection. TRPC1 protein immunohistochemistry score and mRNA expression were of good value in differentiating TSCC tissue from tumor-adjacent tissue and were positively correlated with pathological grade and tumor node metastasis stage. A high TRPC1 protein score was negatively correlated with overall survival, and this correlation was dramatically obvious in patients who received adjuvant radiotherapy. TRPC1 correlates with poor tumor features and unfavorable survival in TSCC patients.
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http://dx.doi.org/10.2217/bmm-2021-0208DOI Listing
August 2022

Nursing Research on Benign Prostatic Hyperplasia Based on Continuous Nursing Care.

Comput Math Methods Med 2022 4;2022:9855770. Epub 2022 Jul 4.

Department of Nephrology, People's Hospital of Dongxihu District, China.

Purpose: To explore the nursing research of prostatic hyperplasia based on continuous nursing and based on the combination of medical care and nursing.

Methods: A prospective study of 96 patients with benign prostatic hyperplasia admitted to our hospital from November 2019 to May 2021 was selected. According to the random number table method, they were divided into an observation group and a control group with 48 cases each. The control group used routine nursing and discharge guidance, and the observation group implemented continuous care based on the combination of medical care and nursing on the basis of the control group. The differences in the scores of quality of life, self-care ability, quality of life score, and sleep quality were compared between the two groups.

Results: After 3 months of nursing, the quality of life scores (health status, psychology, social relationship, environment, physiology, and quality of life scores) of the observation group was higher than those of the control group ( < 0.05). After 3 months of nursing, the quality of life scores of disease, physiology, society, psychology, and satisfaction of the observation group was significantly higher than those of the control group, which was statistically significant ( < 0.05). After 3 months of nursing, the observation group's sleep quality scores in all dimensions (time to fall asleep, sleep time, sleep quality, sleep efficiency, hypnotics, sleep disorders, and day disorders) were higher than those of the control group ( < 0.05). After 3 months of nursing, the observation group's self-care skill score, self-responsibility score, health knowledge score, and self-concept score were better than those of the control group ( < 0.05).

Conclusion: Continued nursing care based on the combination of medical and health care for prostate hyperplasia is beneficial to improve sleep quality and improve the patient's quality of life score and self-care ability and provide certain references for clinical care of patients with benign prostatic hyperplasia.
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http://dx.doi.org/10.1155/2022/9855770DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273421PMC
July 2022

Granal thylakoid structure and function: explaining an enduring mystery of higher plants.

New Phytol 2022 Jul 13. Epub 2022 Jul 13.

School of Integrative Plant Science, Cornell University, Ithaca, NY, 14853, USA.

In higher plants, photosystems II and I are found in grana stacks and unstacked stroma lamellae, respectively. To connect them, electron carriers negotiate tortuous multi-media paths and are subject to macromolecular blocking. Why does evolution select an apparently unnecessary, inefficient bipartition? Here we systematically explain this perplexing phenomenon. We propose that grana stacks, acting like bellows in accordions, increase the degree of ultrastructural control on photosynthesis through thylakoid swelling/shrinking induced by osmotic water fluxes. This control coordinates with variations in stomatal conductance and the turgor of guard cells, which act like an accordion's air button. Thylakoid ultrastructural dynamics regulate macromolecular blocking/collision probability, direct diffusional pathlengths, division of function of Cytochrome  b f complex between linear and cyclic electron transport, luminal pH via osmotic water fluxes, and the separation of pH dynamics between granal and lamellar lumens in response to environmental variations. With the two functionally asymmetrical photosystems located distantly from each other, the ultrastructural control, nonphotochemical quenching, and carbon-reaction feedbacks maximally cooperate to balance electron transport with gas exchange, provide homeostasis in fluctuating light environments, and protect photosystems in drought. Grana stacks represent a dry/high irradiance adaptation of photosynthetic machinery to improve fitness in challenging land environments. Our theory unifies many well-known but seemingly unconnected phenomena of thylakoid structure and function in higher plants.
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http://dx.doi.org/10.1111/nph.18371DOI Listing
July 2022

A validated LC-MS/MS method for the determination of hederasaponin C: Application to pharmacokinetics-pharmacodynamics studies in the therapeutic area of acetic acid-induced ulcerative colitis in rats.

Biomed Chromatogr 2022 Jul 13:e5450. Epub 2022 Jul 13.

Academy of Chinese Medical Sciences, Henan University of Chinese Medicine, Zhengzhou, China.

Hederasaponin C (HSC), one of the main components of Pulsatilla chinensis, is considered as a potential drug for the treatment of inflammatory bowel disease. In the present research, we developed a pharmacokinetics-pharmacodynamics model to describe the concentration-effect course of drug action following the intraperitoneal injection of HSC in colitis rats. A sensitive UPLC-MS/MS method was established for the the determination of HSC in rat plasma to explore the pharmacokinetics properties. The separation was performed on an Accucore C column (2.1 × 100 mm, 2.6 μm) with a flow phase consisting of acetonitrile and 0.1% formic acid water. The assay method was validated and demonstrated good adaptability for application in the pharmacokinetics study. Then the levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β) in colon tissues were measured using an ELISA assay. The levels of TNF-α, IL-1β and IL-6 were decreased after HSC administration, suggesting that HSC can significantly improve the level of inflammatory syndrome factor. The pharmacokinetics study showed that the time to peak concentration of HSC was 1 h. The concentration-effect curves showed a hysteresis loop. There was also a hysteresis between the peaked concentration and the maximum effect of HSC. The present study established in vivo pharmacokinetics-pharmacodynamics models and the results showed a great potential of HSC for treating ulcerative colitis.
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http://dx.doi.org/10.1002/bmc.5450DOI Listing
July 2022

Prediction of Above-Room-Temperature Superconductivity in Lanthanide/Actinide Extreme Superhydrides.

J Am Chem Soc 2022 Jul 12;144(29):13394-13400. Epub 2022 Jul 12.

State Key Laboratory of Superhard Materials and International Center for Computational Method & Software, College of Physics, Jilin University, Changchun 130012, China.

Achieving room-temperature superconductivity has been an enduring scientific pursuit driven by broad fundamental interest and enticing potential applications. The recent discovery of high-pressure clathrate superhydride LaH with superconducting critical temperatures () of 250-260 K made it tantalizingly close to realizing this long-sought goal. Here, we report a remarkable finding based on an advanced crystal structure search method of a new class of extremely hydrogen-rich clathrate superhydride MH (M: rare-earth/actinide atom) stoichiometric compounds stabilized at an experimentally accessible pressure of 350 GPa. These compounds are predicted to host up to 330 K, which is well above room temperature. The bonding and electronic properties of these MH clathrate superhydrides closely resemble those of atomic metallic hydrogen, giving rise to the highest hitherto found in a thermodynamically stable hydride compound. An in-depth study of these extreme superhydrides offers insights for elucidating phonon-mediated superconductivity above room temperature in hydrogen-rich and other low-Z materials.
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http://dx.doi.org/10.1021/jacs.2c05834DOI Listing
July 2022

Source, occurrence and risks of twenty antibiotics in vegetables and soils from facility agriculture through fixed-point monitoring and numerical simulation.

J Environ Manage 2022 Jul 9;319:115652. Epub 2022 Jul 9.

Beijing Key Laboratory of Farmland Soil Pollution Prevention and Remediation, College of Resources and Environmental Sciences, China Agricultural University, Beijing, 100193, PR China. Electronic address:

In this study, a universal method that combined fixed-point monitoring and numerical simulation was used to understand the source, fate and risks of antibiotics in environment. Results showed that the antibiotic concentration in vegetables, soil and manure from 53 fixed-point monitoring sampling sites were ND-18.47, ND-1438.50 and ND-24710.00 μg kg, respectively. There were positive correlations between the antibiotic concentrations of vegetables and soil as well as between soil and manure. The average Amount values were 1.48-46.02, indicating that antibiotics built up pseudo persistent residues in soil due to repeated fertilization. The modified level-III fugacity model showed that tetracyclines and fluoroquinolones tend to remain in soil given their sorption and mobility, while sulfonamides were highly distributed in plants, especially in leaves. Norfloxacin, ofloxacin, sulfadiazine, sulfamethoxazole and sulfisoxazole were found to be risk factors in facility agriculture and should be continuously monitored during agricultural production. Most importantly, we used the inversion method to determine the recommended maximum residue limits of antibiotics in soil. This will not only allow for better control of the amount of the antibiotics in the environment, but also act as a potential method to assess the risks of pollutants without maximum residue limits in the environment.
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http://dx.doi.org/10.1016/j.jenvman.2022.115652DOI Listing
July 2022

Mechanism of Resistance to Pyroxsulam in Multiple-Resistant from China.

Plants (Basel) 2022 Jun 22;11(13). Epub 2022 Jun 22.

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

Black grass ( Huds.) is a highly competitive weed in winter wheat fields of China. Due to repeated use of acetolactate synthase (ALS) inhibitors, many populations have evolved resistance to pyroxsulam in some wheat fields. Research was conducted to determine the molecular basis of herbicide resistance in the AH93 population. Whole-plant dose-response assay confirmed that the AH93 population was resistant to pyroxsulam with a resistance index of 4.2. Cross- and multiple-resistance assays indicated that the AH93 population was cross-resistant to mesosulfuron-methyl and multiple-resistant to pinoxaden. Sequencing of the and gene revealed that there was no target-site mutation in ALS, but Trp-2027-Cys and Cys-2088-Arg amino acid mutations in ACCase in the AH93 population. A malathion pretreatment study indicated that the AH93 population might have cytochrome P450-mediated herbicide metabolic resistance. This is the first report of pyroxsulam resistance in a multiple-resistant population in China, and the Cys-2088-Arg mutation is the first reported case of an ACCase mutant conferring herbicide resistance in .
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http://dx.doi.org/10.3390/plants11131645DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268964PMC
June 2022

Emerging Approach for Fish Freshness Evaluation: Principle, Application and Challenges.

Foods 2022 Jun 26;11(13). Epub 2022 Jun 26.

Department of Food Science and Engineering, Ningbo University, Ningbo 315211, China.

Affected by micro-organisms and endogenous enzymes, fish are highly perishable during storage, processing and transportation. Efficient evaluation of fish freshness to ensure consumer safety and reduce raw material losses has received an increasing amount of attention. Several of the conventional freshness assessment techniques have plenty of shortcomings, such as being destructive, time-consuming and laborious. Recently, various sensors and spectroscopic techniques have shown great potential due to rapid analysis, low sample preparation and cost-effectiveness, and some methods are especially non-destructive and suitable for online or large-scale operations. Non-destructive techniques typically respond to characteristic substances produced by fish during spoilage without destroying the sample. In this review, we summarize, in detail, the principles and applications of emerging approaches for assessing fish freshness including visual indicators derived from intelligent packaging, active sensors, nuclear magnetic resonance (NMR) and optical spectroscopic techniques. Recent developments in emerging technologies have demonstrated their advantages in detecting fish freshness, but some challenges remain in popularization, optimizing sensor selectivity and sensitivity, and the development of algorithms and chemometrics in spectroscopic techniques.
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http://dx.doi.org/10.3390/foods11131897DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9265959PMC
June 2022

Systematic review of mental health symptom changes by sex or gender in early-COVID-19 compared to pre-pandemic.

Sci Rep 2022 Jul 6;12(1):11417. Epub 2022 Jul 6.

Lady Davis Institute for Medical Research, Jewish General Hospital, 3755 Cote Ste-Catherine, Pavilion H4.83, Montréal, QC, H3T 1E2, Canada.

Women and gender-diverse individuals have faced disproportionate socioeconomic burden during COVID-19. There have been reports of greater negative mental health changes compared to men based on cross-sectional research that has not accounted for pre-COVID-19 differences. We compared mental health changes from pre-COVID-19 to during COVID-19 by sex or gender. MEDLINE (Ovid), PsycINFO (Ovid), CINAHL (EBSCO), EMBASE (Ovid), Web of Science Core Collection: Citation Indexes, China National Knowledge Infrastructure, Wanfang, medRxiv (preprints), and Open Science Framework Preprints (preprint server aggregator) were searched to August 30, 2021. Eligible studies included mental health symptom change data by sex or gender. 12 studies (10 unique cohorts) were included, all of which reported dichotomized sex or gender data. 9 cohorts reported results from March to June 2020, and 2 of these also reported on September or November to December 2020. One cohort included data pre-November 2020 data but did not provide dates. Continuous symptom change differences were not statistically significant for depression (standardized mean difference [SMD] = 0.12, 95% CI -0.09-0.33; 4 studies, 4,475 participants; I = 69.0%) and stress (SMD = - 0.10, 95% CI -0.21-0.01; 4 studies, 1,533 participants; I = 0.0%), but anxiety (SMD = 0.15, 95% CI 0.07-0.22; 4 studies, 4,344 participants; I = 3.0%) and general mental health (SMD = 0.15, 95% CI 0.12-0.18; 3 studies, 15,692 participants; I = 0.0%) worsened more among females/women than males/men. There were no significant differences in changes in proportions above cut-offs: anxiety (difference = - 0.05, 95% CI - 0.20-0.11; 1 study, 217 participants), depression (difference = 0.12, 95% CI -0.03-0.28; 1 study, 217 participants), general mental health (difference = - 0.03, 95% CI - 0.09-0.04; 3 studies, 18,985 participants; I = 94.0%), stress (difference = 0.04, 95% CI - 0.10-0.17; 1 study, 217 participants). Mental health outcomes did not differ or were worse by small amounts among women than men during early COVID-19.
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http://dx.doi.org/10.1038/s41598-022-14746-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9258011PMC
July 2022

Multi-Objective Optimization Design of Ladle Refractory Lining Based on Genetic Algorithm.

Front Bioeng Biotechnol 2022 15;10:900655. Epub 2022 Jun 15.

Key Laboratory of Metallurgical Equipment and Control Technology of Ministry of Education, Wuhan University of Science and Technology, Wuhan, China.

Genetic algorithm is widely used in multi-objective mechanical structure optimization. In this paper, a genetic algorithm-based optimization method for ladle refractory lining structure is proposed. First, the parametric finite element model of the new ladle refractory lining is established by using ANSYS Workbench software. The refractory lining is mainly composed of insulating layer, permanent layer and working layer. Secondly, a mathematical model for multi-objective optimization is established to reveal the functional relationship between the maximum equivalent force on the ladle lining, the maximum temperature on the ladle shell, the total mass of the ladle and the structural parameters of the ladle refractory lining. Genetic algorithm translates the optimization process of ladle refractory lining into natural evolution and selection. The optimization results show that, compared with the unoptimized ladle refractory lining structure (insulation layer thickness of 0 mm, permanent layer thickness of 81 mm, and working layer thickness of 152 mm), the refractory lining with insulation layer thickness of 8.02 mm, permanent layer thickness of 76.20 mm, and working layer thickness of 148.61 mm has the best thermal insulation performance and longer service life within the variation of ladle refractory lining structure parameters. Finally, the results of the optimization are verified and analyzed in this paper. The study found that by optimizing the design of the ladle refractory lining, the maximum equivalent force on the ladle lining, the maximum temperature on the ladle shell and the ladle mass were reduced. The thermal insulation performance and the lightweight performance of the ladle are improved, which is very important for improving the service life of the ladle.
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http://dx.doi.org/10.3389/fbioe.2022.900655DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240744PMC
June 2022

The role of Dendrin in IgA Nephropathy.

Nephrol Dial Transplant 2022 Jun 29. Epub 2022 Jun 29.

Department of Clinical Science, Intervention and Technology, Divison of Renal Medicine Karolinska Institutet, Stockholm, Sweden.

Background: IgA nephropathy (IgAN) and its systemic variant IgA vasculitis (IgAV) damage the glomeruli, resulting in proteinuria, hematuria and kidney impairment. Dendrin is a podocyte-specific protein suggested to be involved in the pathogenesis of IgAN. Upon cell injury, dendrin translocates from the slit diaphragm to the nucleus where it is suggested to induce apoptosis and cytoskeletal changes, resulting in proteinuria and accelerated disease progression in mice. Here we investigated gene and protein expression of dendrin in relation to clinical and histopathological findings to further elucidate its role in IgAN/IgAV.

Methods: Glomerular gene expression was measured using microarrays on 30 IgAN/IgAV patients, 5 patients with membranous nephropathy (MN) and 20 deceased kidney donors. Dendrin was spatially evaluated on kidney tissue sections by immunofluorescence (IFL) staining (IgAN patients; n = 4 and nephrectomized kidneys; n = 3) and semi-quantitatively by immunogold electron microscopy (IgAN/IgAV patients; n = 21, MN; n = 5 and living kidney donors; n = 6). Histopathological grading was performed according to Oxford and Banff classifications. Clinical data was collected at the time of biopsy and follow-up.

Results: Dendrin mRNA levels were higher (p = 0.01) in IgA patients compared to MN patients and controls, and most prominently in patients with preserved kidney function and less chronic histopathological changes. Whereas IFL staining did not differ between groups, iEM revealed that a higher relative nuclear dendrin concentration in IgAN patients was associated with slower annual progression rate and milder histopathological changes.

Conclusion: Dendrin mRNA levels and relative nuclear protein concentrations are increased and associated with a more benign phenotype and progression in IgAN/IgAV patients.
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http://dx.doi.org/10.1093/ndt/gfac208DOI Listing
June 2022

Effects of different interventions on internet addiction: A meta-analysis of random controlled trials.

J Affect Disord 2022 09 26;313:56-71. Epub 2022 Jun 26.

Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, Hefei 230032, China; Center for Evidence Based Medicine, Anhui Medical University, Hefei 230032, China. Electronic address:

Objective: To evaluate the effects of different interventions on Internet addiction (IA), a meta-analysis and network meta-analyses were performed.

Methods: We searched PubMed, Cochrane, Embase, Web of Science, PsycINFO, ProQuest, CNKI, WanFang, VIP database, and CBM from their inception to August 2021 to identify randomized controlled trials (RCTs) where the effects of interventions on IA were assessed. The risk of bias was evaluated according to the Revised Cochrane risk-of-bias tool for randomized trials (RoB2). The R studio Software and Stata 14.0 were used to perform traditional meta-analysis and network meta-analyses.

Results: A total of 59 RCTs including 3832 participants were incorporated into meta-analysis. The results of the traditional meta-analysis of 24 studies showed that CBT, group counseling, sports intervention, and Internet-based intervention could significantly reduce IA levels (SMD = -1.90, 95%CI: -2.26 to -1.55, P < 0.01, I = 85.9%) as compared to no-treatment control groups. Network meta-analyses based on different scales showed that combined interventions had the highest probability of being the best interventions for IA (SUCRA = 91.0 % based on IAT; SUCRA = 89.0 % based on CIAS).

Discussion: Most interventions have significant effects on the treatment of IA. Compared with single interventions, combined interventions showed a more pronounced improvement in Internet addiction symptoms.
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http://dx.doi.org/10.1016/j.jad.2022.06.013DOI Listing
September 2022

Association between bedroom light exposure at night and allostatic load among Chinese young adults.

Environ Pollut 2022 Sep 22;308:119671. Epub 2022 Jun 22.

Department of Maternal, Child and Adolescent Health, School of Public Health, Anhui Medical University, Hefei, Anhui, China; Key Laboratory of Population Health Across Life Cycle (Anhui Medical University), Ministry of Education of the People's Republic of China, No 81 Meishan Road, Hefei, 230032, Anhui, China; Anhui Provincial Key Laboratory of Population Health and Aristogenics, Anhui Medical University, No 81 Meishan Road, Hefei, 230032, Anhui, China. Electronic address:

Light at night (LAN) has received increasing attention for its potential health hazards to human and animals. However, to our knowledge, no study has explored the specific effects of bedroom nighttime light exposure on allostatic load (AL). To investigate the association between bedroom individual-level LAN exposure and AL among young adults, an integrative index manifests multiple system dysregulation. Using data from a cohort of 484 Chinese young adults aged 16-22 years. Bedroom light was objectively recorded at 1-min intervals for two nights using a portable illuminance meter. Fasting blood samples were collected at one-year follow-up for the detection of AL parameters. AL score was derived as sum of the top quartile of twelve physiological biomarkers in four systems: metabolic system (BMI, WC, TC, HDL, LDL, TG, HbA1c, INS, GLU); cardiovascular system (SBP, DBP); immune and inflammatory systems (hs-CRP), with HDL was lowest quartile. Univariate and multivariate linear regression models were used to evaluate the association between LAN intensity with AL score and separate AL parameters. The average age of subjects was 18.7 years, 64.3% were female. The mean AL score of LAN group (average LAN intensity ≥ 3lx) was significantly higher than Dim group (3.6 ± 2.6 vs. 2.7 ± 2.1; P = 0.007). For each 1 lx increase of LAN intensity was associated with 0.15-unit increase in AL score (95% CI: 0.06, 0.24; P = 0.001). Moreover, LAN group was associated with increased 1.01-unit in AL score (95% CI: 0.36-1.66; P = 0.003) compared to Dim group. Significant associations between bedroom LAN exposure with allostatic load and separate AL biomarkers were observed in our study. Keeping bedroom darkness at night may be a practicable option to reduce the wear of multiple body systems and improve human cardiometabolic health from early in life.
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http://dx.doi.org/10.1016/j.envpol.2022.119671DOI Listing
September 2022
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