Publications by authors named "Ying Lv"

254 Publications

The pivotal alkyne group in the mutual size-conversion of Au9 with Au10 nanoclusters.

Dalton Trans 2021 Jul;50(29):10113-10118

Anhui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, Key Laboratory of Structure and Functional Regulation of Hybrid Materials of Ministry of Education, Anhui University, Hefei, Anhui, 230601 P. R. China. and Institute of Physical Science and Information Technology, Anhui University, Hefei, Anhui, 230601 P. R. China.

Herein, density functional theory (DFT) calculations were performed to elucidate the mechanism of the reversible single atom size conversion between [Au10(DMPP)4(C6H11C[triple bond, length as m-dash]C)]3+ and [Au9(DMPP)4]3+ (DMPP is 2,2'-bis-(dimethylphosphino)-1,1'-biphenyl, the simplified, theoretical model of the experimentally used 2,2'-bis-(diphenylphosphino)-1,1'-binaphthyl). The presence of a unique alkyne group is pivotal to the nucleophilic attack of the phosphine ligand on the electron-deficient Au10 core. After that, a formal ligand exchange and facile 1,2-P (of the diphosphine ligand) transfer occur to generate the Au9 cluster product. By contrast, the absence of the alkyne group results in a relatively electron-rich Au9 core, and thus an electrophilic attack of the Au(alkyne) complex on the most electron-rich metal sites occurs first. After that, the Au(alkyne) migration on the cluster surface, 1,2-P transfer and core-reconstruction occur successively to generate the thermodynamically highly stable Au10 cluster product.
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http://dx.doi.org/10.1039/d1dt01586aDOI Listing
July 2021

Screening of silicon-activating bacteria and the activation mechanism of silicon in electrolytic manganese residue.

Environ Res 2021 Jul 9;202:111659. Epub 2021 Jul 9.

Civil Engineering Department, University of Nebraska-Lincoln (Omaha Campus), Omaha, NE, 68182, USA.

Electrolytic manganese residue (EMR) is a kind of solid waste with a high silicon content. Most of the silicon in EMR, however, exist in the state of SiO, which cannot be directly absorbed by plants. Currently, it is very challenge to recover the silicon from EMR. In this study, a preliminary screening of strains with silicon-activating ability was conducted, and four strains were screened out and isolated from the soil around the tailings pond of EMR. Then, single factor experiments were conducted to obtain the optimal growth conditions of the four strains, and the results indicated that the Ochrobactrum sp. T-07 had the best silicon-activating ability from EMR after nitrosoguanidine mutagenesis (Ochrobactrum sp. T-07-B). The available silicon (in terms of SiO) in the leaching solution was up to 123.88 mg L, which was significantly higher than that produced by Bacillus circulans and Paenibacillus mucilaginosus, the two commercial available pure culture strains. Results of direct/indirect contact experiments between Ochrobactrum sp. T-07-B and EMR revealed that bioleaching was promoted under the synergistic effect of bacteria growth on the surface of and metabolism within EMR. The newly isolated strains with silicon-activating effect are different from the existing-known silicate bacteria and may be used for more efficient silicon activation in silicate minerals.
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http://dx.doi.org/10.1016/j.envres.2021.111659DOI Listing
July 2021

Polyphenols from blue honeysuckle (Lonicera caerulea var. edulis) berry inhibit lipid accumulation in adipocytes by suppressing lipogenesis.

J Ethnopharmacol 2021 Jul 8;279:114403. Epub 2021 Jul 8.

Department of Cardiology, 2nd Affiliated Hospital of Harbin Medical University, Harbin, 150001, PR China; Key Laboratory of Myocardial Ischemia, Ministry of Education, Harbin Medical University, Harbin, 150001, PR China. Electronic address:

Ethnopharmacological Relevance: Blue honeysuckle (Lonicera caerulea var. edulis) berry has been used in folk medicine for the treatment of bacterial infections, gastrointestinal disorders, and metabolic diseases. There is evidence to support its pharmacological effects in improving diabetes, fatty liver, and obesity.

Aim Of Study: To investigate the effect of blue honeysuckle berry extract (BHBE) on lipid accumulation in adipocytes and the underlying mechanism.

Materials And Methods: High-performance liquid chromatography (HPLC) coupled with mass spectrometry (MS) was applied to analyze the polyphenolic compounds in BHBE. 3T3-L1 cells were used to induce into adipocytes. Oil Red O staining combined with triglyceride (TG) content determination were carried out to evaluate intracellular lipid accumulation. Western blot was used to determine the expression of lipogenic enzymes and transcription factors. Real-time PCR was used to determine the expression of lipolytic enzymes and adipocyte markers.

Results: The primary polyphenols in BHBE are flavonoids (mainly flavonols and anthocyanins). BHBE dose-dependently inhibited lipid accumulation in adipocytes by reducing the expression of fatty acid synthase (FAS) and increasing the phosphorylation level of acetyl-CoA carboxylase (ACC). Moreover, BHBE was found to promote the phosphorylation of AMP-activated protein kinase (AMPK) and further reduce the expression of lipogenic transcription factors (PPARγ, C/EBPα, and SREBP-1c), while the selective AMPK inhibitor attenuated the suppressive effect of BHBE on lipogenesis. In addition, BHBE increased the expression of beige adipocyte markers (Cd137 and Tmem26) and uncoupling protein 1 (UCP1) without affecting the expression of brown adipocyte markers (Ebf3 and Eva1).

Conclusion: BHBE inhibits lipid accumulation in adipocytes by suppressing lipogenesis via AMPK activation as well as by promoting beiging of adipocytes, which supports the anti-obesity potential of blue honeysuckle berry.
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http://dx.doi.org/10.1016/j.jep.2021.114403DOI Listing
July 2021

The effect of warm needle moxibustion on lumbar disc herniation.

Am J Transl Res 2021 15;13(5):5059-5065. Epub 2021 May 15.

Department of Acupuncture and Massage, The Sixth People's Hospital Affiliated to Shanghai Jiaotong University Shanghai, China.

Objective: To investigate the effect of warm needle moxibustion on patients with lumbar disc herniation (LDH).

Methods: A total of 100 patients with lumbar disc herniation treated in our hospital were recruited as the study cohort and randomly assigned into a control group and an observation group. The patients in the control group underwent routine acupuncture treatment, while those in the observation group underwent warm needle moxibustion treatment. The observed indexes, including the clinical efficacy, the visual analogue scale (VAS) scores, the Japanese Orthopedic Association (JOA) scores, the Oswestry disability index (ODI) scores, and the serum inflammatory factor levels were compared between the control group and the observation group.

Results: The total cure rate in the observation group was significantly higher than it was in the control group (76.0% vs 92%, P=0.029). Compared with the patients in the control group after the treatment, the patients' VAS and ODI scores in the observation group were significantly lower, but their JOA scores were significantly higher, and the differences were statistically significant (all P<0.001). Moreover, the serum IL-6 and tumor necrosis factor α (TNF-α) levels in the observation group were remarkably lower than the corresponding levels in the control group (all P<0.001).

Conclusions: Warm needle moxibustion has a significant effect on patients with lumbar disc herniation, because it helps to relieve the pain and other symptoms and reduces the serum inflammatory factor levels.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205828PMC
May 2021

Effective elastic modulus of an intact cornea related to indentation behavior: A comparison between the Hertz model and Johnson-Kendall-Roberts model.

Exp Eye Res 2021 Jun 12;209:108670. Epub 2021 Jun 12.

College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan, China; State Key Lab. of Traction Power, Southwest Jiaotong University, Chengdu, China. Electronic address:

In this study, a macro-indentation test on the submillimeter scale was performed to analyze the indentation behavior of an intact cornea under physiological pressures. The Hertz and Johnson-Kendall-Roberts (JKR) models were employed to solve the elastic modulus (E) of the intact cornea. The relevant detailed analysis showed that the JKR model, which accounted for the contribution from the adhesion energy, could be used to obtain the E values that were more than two-folds of those obtained from the Hertz model, which only considered the external force. Compared with the uniaxial tension test in vitro, unlike the elastic Hertz-model, the E values under physiological pressures that were obtained with the JKR model were between the lower and upper limits of corneal material. This phenomenon indicated that the JKR model could be used to obtain reasonably effective E values of an intact cornea under physiological pressures.
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http://dx.doi.org/10.1016/j.exer.2021.108670DOI Listing
June 2021

Effect of Daily Iron Supplementation on Infantile Iron Homeostasis in Preterm Infants.

Front Pediatr 2021 28;9:687119. Epub 2021 May 28.

Department of Pediatric Health Care, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, China.

The aim of this study was to investigate the effects of unified iron supplementation and identify the factors related to the iron homeostasis among preterm infants. A total of 250 preterm infants were divided into neonatal anemic (NA, = 154) and non-neonatal anemic group (NNA, = 96). Iron supplements at a dose of 2 mg/kg per day were given from 40 weeks' gestational age to 6 months. Iron status parameters were measured at 3 and 6 months, respectively. Prevalence of iron deficiency (ID) and iron deficiency anemia (IDA), and the correlated factors were analyzed. Growth and side-effects were monitored. There were no significant differences for the prevalence of ID or IDA between the two groups. Multivariate regression analyses showed that higher Hb at birth and early treatment of blood transfusion reduced the risk of ID/IDA at 3 months (all < 0.05); while higher level of Hb at 3 months ( = 0.004) and formula feeding reduced the occurrence of ID/IDA at 6 months ( < 0.05); males had a 3.35 times higher risk to develop ID/IDA than girls ( = 0.021). No differences in growth and side effects were found. A daily dose of 2 mg/kg iron supplement is beneficial to maintain iron homeostasis in majority preterm infants within 6 months regardless of their neonatal anemia history. Under the routine iron supplementation, Hb level at birth and at 3 months, early treatment of blood transfusion, gender and feeding patterns are the major factors affecting the prevalence of ID/IDA among preterm infants in infancy.
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http://dx.doi.org/10.3389/fped.2021.687119DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8192839PMC
May 2021

[Effect of electroacupuncture on lumbar disc herniation with different multifidus fatty infiltration rates].

Zhongguo Zhen Jiu 2021 Jun;41(6):593-7

School of Acupuncture-Moxibustion, Tuina and Rehabilitation, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu Province.

Objective: To observe the therapeutic effect of electroacupuncture on lumbar disc herniation (LDH) with different multifidus fatty infiltration rates.

Methods: A total of 108 eligible LDH patients received MRI test before treatment and the multifidus fatty infiltration rate (FI) was measured. According to theresults, they were divided to group A (FI<10%), group B (10%≤FI≤20%) and group C (FI>20%), 36 cases in each one. EA was provided in all of the groups. The selected acupoints were Jiaji (EX-B 2) from L to S and Dachangshu (BL 25), etc. on the affected side. EA was applied to Jiaji (EX-B 2) at L and L on the affected side (continuous wave, 20 Hz in frequency), retained for 20 min. The treatment was given once every two days, for 10 times totally. Before and after treatment, the score of Oswestry disability index (ODI) and the scores of physical component summary (PCS) and mental component summary (MCS) of medical outcomes survey short form-36 (SF-36) scale were observed in each group separately.

Results: Compared with before treatment, ODI score was reduced (<0.05) and the scores of PCS and MCS were increased (<0.05) after treatment in the three groups. After treatment, ODI score in the group A was lower than that in either the group B or the group C (<0.05) and the score in the group B was lower than that in the group C (<0.05). PCS score in the group A was higher than that in either the group B or the group C (<0.05) and the score in the group B was higher than the group C (<0.05).

Conclusion: Electroacupuncture relieves pain and improves physical and psychological health of LDH patients. Multifidus fatty infiltration rate is probably one of the factors to influence the therapeutic effect of electroacupuncture.
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http://dx.doi.org/10.13703/j.0255-2930.20200817-k0006DOI Listing
June 2021

Changes in the secondary structures and zeta potential of soybean peptide and its calcium complexes in different solution environments.

Food Funct 2021 Jul 25;12(13):5967-5974. Epub 2021 May 25.

College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China.

To illustrate the relationship between environment hydrophobicity and soybean peptide and its calcium complexes when they are absorbed transmembrane, different solution environments (HBS buffer, TFE hydrophobic solution and cell suspension) were used to simulate hydrophilic and hydrophobic environments. In this study, soybean peptides (10-30 kDa) with a high calcium binding capacity were prepared by enzymatic hydrolysis and ultrafiltration. The results of cell experiments showed that the peptide could transport calcium into cells for absorption. Secondary structure changes of the peptide and its calcium complexes in different solution environments showed that the secondary structure of the peptide changed during the transmembrane absorption, and the contents of α-helix and β-sheet structures increased. Besides, the β-sheet structures in the peptide-calcium complexes were further converted to an α-helix structure. This conversion may be induced by the hydrophobicity of peptide solutions. In addition, when the conformation changes, the positively charged peptides in the sample will be exposed and then interact with cells, which is beneficial for the transmembrane of peptide-calcium complexes.
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http://dx.doi.org/10.1039/d0fo03478aDOI Listing
July 2021

Plasma-Derived Exosomal ALIX as a Novel Biomarker for Diagnosis and Classification of Pancreatic Cancer.

Front Oncol 2021 5;11:628346. Epub 2021 May 5.

Department of Gastroenterology, Nanjing University Medical School Affiliated Nanjing Drum Tower Hospital, Nanjing, China.

Background: Pancreatic cancer (PC) has a dismal prognosis due to its insidious early symptoms and poor early detection rate. Exosomes can be released by various cell types and tend to be a potential novel biomarker for PC detection. In this study, we explored the proteomic profiles of plasma exosomes collected from patients with PC at different stages and other pancreatic diseases.

Methods: Plasma samples were collected from six groups of patients, including PC at stage I/II, PC at stage III/IV, well-differentiated pancreatic neuroendocrine tumor (P-NET), pancreatic cystic lesions (PCLs), chronic pancreatitis (CP), and healthy controls (HCs). Plasma-derived exosomes were isolated by ultracentrifugation and identified routinely. Isobaric tags for relative and absolute quantification (iTRAQ) based proteomic analysis along with bioinformatic analysis were performed to elucidate the biological functions of proteins. The expression of exosomal ALIX was further confirmed by enzyme-linked immunosorbent assay in a larger cohort of patients. Furthermore, receiver operating characteristic curve analysis was applied to evaluate the potential of ALIX as a novel diagnostic biomarker.

Results: The proteomic profile revealed a total of 623 proteins expressed among the six groups, and 16 proteins with differential degrees of abundance were found in PC other pancreatic diseases (including P-NET, PCLs, and CP). Based on the results of proteomic and bioinformatic analyses, exosomal ALIX was subsequently selected as a novel biomarker for PC detection and validated in another clinical cohort. We noticed that ALIX expression was elevated in PC patients compared with patients with other pancreatic diseases or HC, and it was also closely associated with TNM stage and distant metastasis. Interestingly, the combination of exosomal ALIX and serum CA199 has greater values in differentiating both early late PC (AUC value 0.872) and PC other pancreatic diseases (AUC value 0.910) than either ALIX or CA199 alone.

Conclusion: In summary, our study demonstrated that based on proteomic profiling, proteins isolated from the plasma-derived exosomes may function as ideal non-invasive biomarkers for the clinical diagnosis of PC. Importantly, exosomal ALIX combined with CA199 has great potentials in detection of PC, especially in distinguishing PC patients at early stages from advanced stages.
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http://dx.doi.org/10.3389/fonc.2021.628346DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8131866PMC
May 2021

Ultra-selective ion sieve for thorium recovery from rare earth elements using oxygen-rich microporous carbon adsorption.

J Hazard Mater 2021 Sep 15;417:126115. Epub 2021 May 15.

Department of Chemistry and Macromolecules Innovation Institute, Virginia Tech, Blacksburg, VA 24061, United States; School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, PR China. Electronic address:

The ultra-selective extraction of thorium ions (Th(IV)) from lanthanides is of significance to both solve the radioactive pollution issue in rare earth (RE) production and sustainably provide thorium fuel for the liquid fluoride thorium reactors (LFTR). However, it remains a great challenge. Here, we reported an oxygen-rich microporous carbon for ultra-selective extraction of Th(IV) from rare earth elements (REEs) in a wide pH range. This selectivity was derived from the synergy of the oxygen-rich nature, microporous structure of the carbons, the chemical valence, and the ionic size of Th(IV) species. This oxygen-rich microporous carbon presented an ultra-high distribution coefficient (K) of 1.15 × 10 mL g for Th(IV) at pH 4.9 in the presence of 15 REEs and revealed outstanding performance for Th(IV) extraction from three simulated RE solutions with high ionic strength of lanthanides. Meanwhile, an exceptional adsorption capacity of 624.98 mg g was obtained in the single Th(IV) solution. Both values were superior to those of reported adsorbents. More importantly, the new adsorbent developed here could be prepared from cigarette butts. These features ensured the oxygen-rich carbon as a promising and cost-effective adsorbent for high-purity thorium extraction from REEs.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126115DOI Listing
September 2021

Effects of a 4-year intervention on hand hygiene compliance and incidence of healthcare associated infections: a longitudinal study.

Infection 2021 May 14. Epub 2021 May 14.

School of Public Health, Zhengzhou University, 100 Kexue Avenue, Gaoxin District, Zhengzhou, 450001, China.

Purpose: Studies have demonstrated improvements in hand hygiene (HH) compliance through interventions, noting the negative association of HH and healthcare associated infections (HAIs), but how to sustain long-term improvement is still unknown in the Chinese population. This study sought to determine the extent of change in HH compliance after multimodal HH interventions, and to evaluate the relationship between that change and HAI incidence.

Methods: We conducted a longitudinal study in a general teaching hospital in China from 2017 to 2020. Trained investigators observed HH practices based on the World Health Organization's 5 moments for HH. We identified the incidence of HAIs using semi-automated constant surveillance software. We used the Cochran-Mantel-Haenszel test to assess the secular trend of HH compliance and HAIs. The Spearman correlation coefficient (r) was used to evaluate the relationship between the incidence of HAIs and compliance with HH.

Results: The study made 480,943 observations, where HH was occurring between 2017 and 2020. HH compliance increased from 68.90 to 91.76% during that period (P < 0.01), while the incidence of HAIs decreased from 1.10 to 0.91%. Compliance also increased for each moment type and for each healthcare worker (P < 0.01). Lower HH compliance was observed in before-patient contact and after contact with patient surroundings, and among interns and cleaners. We also observed a weak but statistically significant negative correlation between the monthly HH compliance and incidence of HAIs (r =  - 0.27; P = 0.037).

Conclusions: The multimodal HH implementation delivered sustained improvement in HH compliance, and this change was associated with a decline in the incidence of HAIs.
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http://dx.doi.org/10.1007/s15010-021-01626-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120257PMC
May 2021

Differential prognostic implications of gastric adenocarcinoma based on Lauren's classification: a Surveillance, Epidemiology, and End Results (SEER)-based cohort study.

Ann Transl Med 2021 Apr;9(8):646

Department of Gastroenterology, Nanjing Drum Tower Hospital, Affiliated Drum Tower Hospital, Medical School of Nanjing University, Nanjing, China.

Background: Our study aims to analyze the association between Lauren's classification and gastric adenocarcinoma prognosis using comprehensive statistical analyses.

Methods: According to the selection criteria, patients were included from the Surveillance, Epidemiology, and End Results (SEER) database. Univariate and multivariate Cox regression, propensity score matching, and a multivariate competing risk model were used to investigate the association between Lauren's classification and prognosis. Subgroup analysis was used to investigate the role of confounding factors on the association between Lauren types and prognosis.

Results: After exclusion, a total of 20,218 patients from the SEER database were included, with 14,374 intestinal types and 5,844 diffuse types. The univariate Cox regression analysis revealed that the diffuse type had a poorer cancer-specific survival (CSS) rate [hazard ratio (HR), 1.44; 95% confidence interval (CI), 1.38-1.50]. After adjusting for confounding variables, the diffuse type also showed a higher risk of cancer-specific death (HR, 1.20; 95% CI, 1.15-1.20). Sensitivity analysis showed that after propensity score matching, the diffuse type had a poorer CSS rate (HR, 1.23; 95% CI, 1.10-1.36), and the competing risk model further validated these results [subdistribution HR (SHR), 1.32; 95% CI, 1.23-1.41]. Moreover, subgroup analysis demonstrated stable results in the subgroups, except for patients with T1 stage (HR, 1.06; 95% CI, 0.87-1.28) and a tumor size <2 cm (HR, 1.00; 95% CI, 0.83-1.21).

Conclusions: Diffuse-type gastric adenocarcinoma had an overall poorer prognosis compared to the intestinal type. However, in patients with T1 stage and tumor size <2 cm, the diffuse type had a comparable survival rate with the intestinal type.
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http://dx.doi.org/10.21037/atm-20-7953DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106066PMC
April 2021

Gastric metastasis from pancreatic cancer characterized by mucosal erosion: a case report and literature review.

J Int Med Res 2021 Apr;49(4):3000605211003759

Department of Gastroenterology, Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University, Nanjing, Jiangsu, China.

Pancreatic cancer with gastrointestinal tract metastasis is a fairly rare occurrence, and gastric metastasis in such cases has been seldom reported. We herein present a case of gastric involvement secondary to pancreatic cancer in a 74-year-old woman in whom the metastatic lesion only presented as mucosal erosion in the stomach. The patient had a 1-month history of progressive right upper quadrant pain before admission. Computed tomography and endoscopic examinations revealed a solid and hypo-enhancing mass in the head of the pancreas. The patient underwent conventional upper endoscopy before pancreatic biopsy, and mucosal erosion was observed in the gastric pylorus. We obtained gastric and pancreatic biopsies by gastroscopy and endoscopic ultrasound-guided fine needle aspiration, respectively. Pathologically, the biopsies taken from the area of gastric erosion showed poorly differentiated invasive adenocarcinoma that was morphologically consistent with the pancreatic specimens. Moreover, the gastric section showed tumor thrombi within the vessels. Hence, the suspected diagnosis was unresectable pancreatic cancer with gastric metastasis. The patient immediately underwent two courses of chemotherapy, but her condition rapidly deteriorated and she died 2 months later.
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http://dx.doi.org/10.1177/03000605211003759DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044569PMC
April 2021

Silencing XIST mitigated lipopolysaccharide (LPS)-induced inflammatory injury in human lung fibroblast WI-38 cells through modulating miR-30b-5p/CCL16 axis and TLR4/NF-κB signaling pathway.

Open Life Sci 2021 6;16(1):108-127. Epub 2021 Feb 6.

Department of Pediatrics, Guangdong Second Hospital of Traditional Chinese Medicine, No. 60 Hengfu Road, 510000, Guangzhou, Guangdong, China.

Background: Emerging evidence shows that long noncoding RNA (lncRNA) has been a novel insight in various diseases, including pneumonia. Even though lncRNA X-inactive-specific transcript (XIST) is well studied, its role in pneumonia remains to be largely unrevealed.

Methods: Expression of XIST, miRNA-30b-5p (miR-30b-5p), and CC chemokine ligand 16 (CCL16) was detected using reverse transcriptase quantitative polymerase chain reaction and western blotting; their interaction was confirmed by dual-luciferase reporter assay. Apoptosis, inflammation, and toll-like receptor 4 (TLR4)/NF-κB signaling pathway were measured using methyl thiazolyl tetrazolium assay, flow cytometry, western blotting, and enzyme-linked immunosorbent assay.

Results: Lipopolysaccharide (LPS) stimulation decreased cell viability and B cell lymphoma (Bcl)-2 expression, and increased cell apoptosis rate and expression of Bcl-2-associated X protein (Bax), cleaved-caspase-3, interleukin (IL)-6, IL-1β, and tumor necrosis factor α (TNF-α) in WI-38 cells. Expression of XIST and CCL16 was upregulated in the serum of patients with pneumonia and LPS-induced WI-38 cells, respectively; silencing XIST and CCL16 could suppress LPS-induced apoptosis and inflammation in WI-38 cells, and this protection was abolished by miR-30b-5p downregulation. Moreover, XIST and CCL16 could physically bind to miR-30b-5p, and XIST regulated CCL16 expression via sponging miR-30b-5p. TLR4 and phosphorylated P65 (p-P65) and p-IκB-α were highly induced by LPS treatment, and this upregulation was diminished by blocking XIST, accompanied with CCL16 downregulation and miR-30b-5p upregulation.

Conclusions: Silencing XIST could alleviate LPS-induced inflammatory injury in human lung fibroblast WI-38 cells through modulating miR-30b-5p/CCL16 axis and inhibiting TLR4/NF-κB signaling pathway.
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http://dx.doi.org/10.1515/biol-2021-0005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7988359PMC
February 2021

Redox-Induced Interconversion of Two Au Nanoclusters: the Mechanism and the Structure-Bond Dissociation Activity Correlations.

Inorg Chem 2021 Apr 29;60(8):5724-5733. Epub 2021 Mar 29.

Department of Chemistry and Centre for Atomic Engineering of Advanced Materials, Anhui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, Key Laboratory of Structure and Functional Regulation of Hybrid Materials of Ministry of Education, Anhui University, Hefei, Anhui 230601, P. R. China.

The interconversion of atomically precise nanoclusters represents an excellent platform to understand the structural correlations of nanomaterials at the atomic level. Herein, density functional theory calculations were performed to elucidate the mechanism of the redox-induced interconversion of [Au(dppp)] and [Au(dppp)Cl] (dppp is short for 1,3-bis(diphenylphosphino)propane) nanoclusters. Reduction is the driving force for the conversion of [Au(dppp)Cl] to [Au(dppp)], while the Au-Au and first Au-Cl bond dissociations occur asynchronously on the two different corner Au atoms to avoid the formation of an electron-deficient Au atom. By contrast, the reduced electron density of [Au(dppp)] by oxidation with O weakens the outmost Au-Au bond therein and facilitates the coordination of the electron-rich chloride(s). The reduction- and oxidation-induced activations, respectively, of Au-Cl and Au-Au bonds and the elucidated principles on the structure-activity correlations might also be generalized to other size conversions upon redox treatment.
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http://dx.doi.org/10.1021/acs.inorgchem.0c03828DOI Listing
April 2021

Rapid SARS-CoV-2 antigen detection potentiates early diagnosis of COVID-19 disease.

Biosci Trends 2021 May 26;15(2):93-99. Epub 2021 Mar 26.

Department of Infectious Disease, Shanghai Public Health Clinical Center, Shanghai, China.

As the COVID-19 epidemic is still ongoing, a more rapid detection of SARS-CoV-2 infection such as viral antigen-detection needs to be evaluated for early diagnosis of COVID-19 disease. Here, we report the dynamic changes of SARS-CoV-2 viral antigens in nasopharyngeal swabs of COVID-19 patients and its association with the viral nucleic acid clearance and clinical outcomes. Eighty-five COVID-19 patients were enrolled for detection of SARS-CoV-2 viral antigens, including 57 anti-SARS-CoV-2 antibody negative cases and 28 antibody positive cases. The viral antigen could be detected in 52.63% (30/57) patients with SARS-CoV-2 antibody negative at the early stage of SARS-CoV-2 infection, especially in the first 5 days after disease onset (p = 0.0018) and disappeared in about 8 days after disease onset. Viral antigens were highly detectable in patients with low Ct value (less than 30) of SARS-CoV-2 nucleic acid RT-PCT assay, suggesting the expression of viral antigen was associated with high viral load. Furthermore, positive antigen detection indicated disease progression, nine cases with positive antigen (9/30, 30.0%), in contrast to two cases (2/27, 7.40%) (p = 0.0444) with negative antigen, which progressed into severe disease. Thus, the viral antigens were persistent in early stages of infection when virus was in highly replicating status, and viral antigen detection promises to rapidly screen positive patients in the early stage of SARS-CoV-2 infection.
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http://dx.doi.org/10.5582/bst.2021.01090DOI Listing
May 2021

Clinical outcomes of early gastric cardiac cancer treated with endoscopic submucosal dissection in patients with different indications.

BMC Gastroenterol 2021 Mar 12;21(1):119. Epub 2021 Mar 12.

Department of Gastroenterology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, China.

Background: Endoscopic submucosal dissection (ESD) has been accepted as a standard treatment for early gastric cardiac cancer (EGCC). Here, we investigate the clinical outcomes of the EGCC patients who underwent ESD in different indications.

Methods: From January 2011 to October 2019, we enrolled 502 EGCC lesions from 495 patients which were resected by ESD at our center. We retrospectively analyzed the short-term and long-term clinical outcomes among different indication groups.

Results: The number of the patients in the absolute indication (AI), expanded indication (EI) and beyond the expanded indication (BEI) groups was 265, 137 and 93, respectively. The en bloc resection rate was 100%, 100% and 98.9% (P = 0.185). The complete resection rate was 99.3%, 98.5% and 74.5%, respectively (P < 0.001). During a median follow-up of 48.1 months, the lymph node metastasis rate was 0%, 0% and 2.3% (P < 0.001). The distant metastasis rate was 0.4%, 0% and 2.3% (P = 0.150). The five-year disease-specific survival rate in the BEI group was 96.6% (P = 0.016), compared to 99.6% in the AI group and 100% in the EI group.

Conclusion: The efficacy for ESD patients in EI group was almost equal to the AI group. Patients in the BEI group showed generally favorable clinical outcomes and needed to be carefully checked after ESD. ESD may be an optional treatment for patients unsuitable for gastrectomy.
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http://dx.doi.org/10.1186/s12876-021-01700-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7953789PMC
March 2021

Analysis of T-cell receptor repertoire in peripheral blood of patients with pancreatic cancer and other pancreatic diseases.

J Cell Mol Med 2021 Apr 8;25(8):3991-4000. Epub 2021 Mar 8.

Department of Gastroenterology, Nanjing University Medical School Affiliated Drum Tower Hospital, Nanjing, China.

Pancreatic cancer (PC) has been the fourth cancer-related death worldwide, diagnosed at an unresectable stage due to its rapid progression and few symptoms of this disease at early stages. The aim of this study was to determine the association between the diversity of T-cell receptor (TCR) repertoire and clinicopathological characteristics of patients with PC and other benign pancreatic diseases. In order to make a comprehensive analysis the TCR repertoire, high-throughput sequencing was used to differentiate complementarity determining region 3 (CDR3) of the TCR β chain in peripheral blood samples from 3 PC, 3 chronic pancreatitis, 3 pancreatic cystic lesions and 3 pancreatic neuroendocrine tumour patients. We found that there were significant differences related to TCR repertoire between PC and other pancreatic diseases, and PC is a relatively immunosuppressive tumour. Changes of peripheral TCR repertoire may be used to predict the progression of PC and the response to immunotherapy. And there may exist novel-specific antigens in PC patients which could be used to design targeting immunotherapy in the nearly future.
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http://dx.doi.org/10.1111/jcmm.16358DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8051704PMC
April 2021

Hypoxia promotes the metastasis of pancreatic cancer through regulating NOX4/KDM5A-mediated histone methylation modification changes in a HIF1A-independent manner.

Clin Epigenetics 2021 01 26;13(1):18. Epub 2021 Jan 26.

Department of Gastroenterology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, No. 321 Zhongshan Road, Nanjing, 210008, Jiangsu, China.

Background: Hypoxia is a characteristic of the tumor microenvironments within pancreatic cancer (PC), which has been linked to its malignancy. Recently, hypoxia has been reported to regulate the activity of important carcinogenic pathways by changing the status of histone modification. NOX4, a member of NADPH oxidase (NOX), has been found to be activated by hypoxia and promote cancer progression in several cancers. But whether it is involved in the epigenetic changes of tumor cells induced by hypoxia is still unclear, and its biological roles in PC also need to be explored.

Methods: A hypoxic-related gene signature and its associated pathways in PC were identified by analyzing the pancreatic cancer gene expression data from GEO and TCGA database. Candidate downstream gene (NOX4), responding to hypoxia, was validated by RT-PCR and western blot. Then, we evaluated the relationship between NOX4 expression and clinicopathologic parameters in 56 PC patients from our center. In vitro and in vivo assays were preformed to explore the phenotype of NOX4 in PC. Immunofluorescence, western blot and chromatin immunoprecipitation assays were further applied to search for a detailed mechanism.

Results: We quantified hypoxia and developed a hypoxia signature, which was associated with worse prognosis and elevated malignant potential in PC. Furthermore, we found that NADPH oxidase 4 (NOX4), which was induced by hypoxia and upregulated in PC in a HIF1A-independent manner, caused inactivation of lysine demethylase 5A (KDM5A), increased the methylation modification of histone H3 and regulated the transcription of EMT-associated gene_ snail family transcriptional repressor 1 (SNAIL1). This served to promote the invasion and metastasis of PC. NOX4 deficiency repressed hypoxia-induced EMT, reduced expression of H3K4ME3 and impaired the invasion and metastasis of PC cells; however, knockdown of KDM5A reversed the poor expression of H3KEME3 induced by NOX4 deficiency, thereby promoting EMT.

Conclusions: This study highlights the prognostic role of hypoxia-related genes in PC and strong correlation with EMT pathway. Our results also creatively discovered that NOX4 was an essential mediator for hypoxia-induced histone methylation modification and EMT in PC cells.
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http://dx.doi.org/10.1186/s13148-021-01016-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7836598PMC
January 2021

Modulation of the HMGB1/TLR4/NF-κB signaling pathway in the CNS by matrine in experimental autoimmune encephalomyelitis.

J Neuroimmunol 2021 03 16;352:577480. Epub 2021 Jan 16.

Department of Pharmacy, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China. Electronic address:

The inflammatory mediator high-mobility group box 1 (HMGB1)-induced signaling pathway has been shown to play an important role in the pathogenesis of multiple sclerosis (MS) and its animal model, experimental autoimmune encephalomyelitis (EAE). Matrine (MAT), a quinolizidine alkaloid component derived from the root of Sophorae flavescens, has the capacity to effectively suppress EAE. However, the impact of MAT treatment on HMGB1-induced signaling is not known. In the present study, we show that MAT treatment alleviated disease severity of ongoing EAE, reduced inflammatory infiltration and demyelination, and reduced the production of inflammatory factors including TNF-α, IL-6, and IL-1β in the CNS. Moreover, MAT administration significantly reduced the protein and RNA expression of HMGB1 and TLR4 in the spinal cord, particularly in astrocytes and microglia/infiltrating macrophages. The expression of MyD88 and TRAF6, and the phosphorylation of NF-κB p65, was also down-regulated after MAT treatment. In contrast, the level of IκB-α, an inhibitory molecule for NF-κB activation, was significantly increased. Furthermore, the direct inhibitory effect of MAT on HMGB1/TLR4/NF-κB signaling in macrophages was further confirmed in vitro. Taken together, these findings demonstrate that MAT treatment alleviated CNS inflammatory demyelination and activation of astrocytes and microglia/macrophages in EAE rats, and that the mechanism underlying these effects may be closely related to modulation of HMGB1/TLR4/NF-κB signaling pathway.
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http://dx.doi.org/10.1016/j.jneuroim.2021.577480DOI Listing
March 2021

Factors influencing development of non-cardiac chest pain after endoscopic submucosal dissection for esophageal neoplasms: a retrospective case-control study of 309 patients from a single center.

Dis Esophagus 2021 Jan 14. Epub 2021 Jan 14.

Department of Gastroenterology, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, China.

Endoscopic submucosal dissection (ESD) is widely used for early stage esophageal cancer and precancerous lesions. Non-cardiac chest pain (NCCP) is a frequent complication of ESD. However, little is known about its incidence and associated factors. This study investigated the pain incidence and predictive factors for pain development after ESD for esophageal neoplasms. We enrolled a total of 309 patients with esophageal neoplasms, who underwent ESD in our center from January 2018 to June 2019. Sociodemographic and clinicopathological information for all patients was collected, and patients were divided into either a pain-free group (n = 156) or a pain group (n = 153) according to whether there was onset of NCCP 24-48 hours after surgery. We made comparisons between groups using Student's t test or the χ2 test. Logistic-regression analysis was used to screen for risk factors. There were statistically significant differences in histories of previous surgery (P = 0.039), lesion size (P = 0.026), operation time (P = 0.009), and postoperative fever (P = 0.001). History of previous surgery (P = 0.043) and postoperative fever (P = 0.007) were independent risk factors for chest pain after esophageal ESD treatment. Chest pain and fever prolonged postoperative hospitalization time (P = 0.005, P = 0.001) and increased hospitalization cost (P = 0.034, P < 0.001). A history of previous surgery and postoperative fever was associated with the occurrence of NCCP after ESD in patients with esophageal neoplasms. NCCP and fever after esophageal ESD increased both hospitalization time and cost.
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http://dx.doi.org/10.1093/dote/doaa126DOI Listing
January 2021

A combination of CMC and α-MSH inhibited ROS activated NLRP3 inflammasome in hyperosmolarity stressed HCECs and scopolamine-induced dry eye rats.

Sci Rep 2021 01 13;11(1):1184. Epub 2021 Jan 13.

Tianjin Medical University Eye Hospital, Eye Institute and School of Optometry, Tianjin International Joint Research and Development Centre of Ophthalmology and Vision Science, Tianjin, 300384, China.

An important mechanism involved in dry eye (DE) is the association between tear hyperosmolarity and inflammation severity. Inflammation in DE might be mediated by the NLRP3 inflammasome, which activated by exposure to reactive oxygen species (ROS). A combination of carboxymethylcellulose (CMC) and α-melanocyte stimulating hormone (α-MSH) may influence DE through this mechanism, thus avoiding defects of signal drug. In this study, we assessed whether treatment comprising CMC combined with α-MSH could ameliorate ocular surface function; we found that it promoted tear secretion, reduced the density of fluorescein sodium staining, enhanced the number of conjunctival goblet cells, and reduced the number of corneal apoptotic cells. Investigation of the underlying mechanism suggested that the synergistic effect of combined treatment alleviated DE inflammation through reduction of ROS level and inhibition of the NLRP3 inflammasome in human corneal epithelial cells. These findings indicate that combined CMC + α-MSH treatment could ameliorate lesions and restore ocular surface function in patients with DE through reduction of ROS level and inhibition of NLRP3 signalling.
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http://dx.doi.org/10.1038/s41598-020-80849-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7807058PMC
January 2021

Effect of Tai Chi and Resistance Training on Cancer-Related Fatigue and Quality of Life in Middle-Aged and Elderly Cancer Patients.

Chin J Integr Med 2021 Apr 9;27(4):265-272. Epub 2021 Jan 9.

Department of Rehabilitation Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, China.

Objective: To study the effect of Tai Chi (TC) and resistance training (RT) with different intensity on the cancer-related fatigue (CRF) and quality of life (QoL) of middle-aged and elderly cancer patients.

Methods: Totally 120 cancer patients were enrolled and randomly assigned to 4 groups by a random number table, including TC group, high-intensity 60% one repetition maximum (1-RM) RT group (HIRT), low-intensity (30% 1-RM) RT group (LIRT) and control group, 30 patients in each group. Participants in the TC group received 24-form simplified Yang-style TC training at a frequency of 40 min per day, 3 days per week for 12 weeks. Patients in the two RT groups received 10 sessions, 6 designated movements per day, 3 days per week for 12 weeks. The 1-RM of 6 muscle groups, fat mass (FM), lean body mass (LBM), along with the scores of Brief Fatigue Inventory (BFI), QoL questionnaire for Chinese cancer patients receiving chemobiotherapy (QLQ-CCC), Generalized Anxiety Disorder-7 (GAD-7), Patient Health Questionnaire-9 (PHQ-9) and Pittsburgh Sleep Quality Index (PSQI) were measured before and after training. The adverse effect was also observed.

Results: After 12-week intervention, patients in both TC and RT groups showed significant improvements in CRF and QLQ-CCC compared to pre-treatment (P<0.05). Compared with the LIRT and TC groups, patients in the HIRT group improved more significantly in increasing muscle strength and LBM, and reducing in FM (P<0.05). Patients in the TC group significantly increased in lower limb muscle strength compared with the LIRT group (P<0.05). In addition, patients in the TC group showed more significant improvements in scores of GAD-7, PHQ-9 and PSQI than 2 RT groups (P<0.05).

Conclusions: TC and RT, both low- and high-intensity training, can significantly increase muscle strength, reduce CRF and improve QoL in the middle-aged and elderly cancer patients. TC has a better effect than RT in terms of sleep quality and mental health. The long-term application is needed to substantiate the effect of TC as an alternative exercise in cancer patients.
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http://dx.doi.org/10.1007/s11655-021-3278-9DOI Listing
April 2021

Current Evidence of Interleukin-6 Signaling Inhibitors in Patients With COVID-19: A Systematic Review and Meta-Analysis.

Front Pharmacol 2020 15;11:615972. Epub 2020 Dec 15.

Department of Emergency Medicine, Qilu Hospital of Shandong University, Jinan, China.

Interleukin-6 (IL-6) is known to be detrimental in coronavirus disease 2019 (COVID-19) because of its involvement in driving cytokine storm. This systematic review and meta-analysis aimed to assess the safety and efficacy of anti-IL-6 signaling (anti-IL6/IL-6R/JAK) agents on COVID-19 based on the current evidence. Studies were identified through systematic searches of PubMed, EMBASE, ISI Web of Science, Cochrane library, ongoing clinical trial registries (clinicaltrials.gov), and preprint servers (medRxiv, ChinaXiv) on August 10, 2020, as well as eligibility checks according to predefined selection criteria. Statistical analysis was performed using Review Manager (version 5.3) and STATA 12.0. Thirty-one studies were included in the pooled analysis of mortality, and 12 studies were identified for the analysis of risk of secondary infections. For mortality analysis, 5630 COVID-19 cases including 2,132 treated patients and 3,498 controls were analyzed. Anti-IL-6 signaling agents plus standard of care (SOC) significantly decreased the mortality rate compared to SOC alone (pooled OR = 0.61, 95% CI 0.45-0.84, = 0.002). For the analysis of secondary infection risk, 1,624 patients with COVID-19 including 639 treated patients and 985 controls were included, showing that anti-IL-6 signaling agents did not increase the rate of secondary infections (pooled OR = 1.21, 95% CI 0.70-2.08, = 0.50). By contrast, for patients with critical COVID-19 disease, anti-IL-6 signaling agents failed to reduce mortality compared to SOC alone (pooled OR = 0.75, 95% CI 0.42-1.33, = 0.33), but they tended to increase the risk of secondary infections (pooled OR = 1.85, 95% CI 0.95-3.61, = 0.07). A blockade of IL-6 signaling failed to reduce the mechanical ventilation rate, ICU admission rate, or elevate the clinical improvement rate. IL-6 signaling inhibitors reduced the mortality rate without increasing secondary infections in patients with COVID-19 based on current studies. For patients with critical disease, IL-6 signaling inhibitors did not exhibit any benefit.
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http://dx.doi.org/10.3389/fphar.2020.615972DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7769953PMC
December 2020

Response.

Authors:
Wei Zhang Ying Lv

Gastrointest Endosc 2021 01;93(1):287-288

Department of Gastroenterology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, 210008, Nanjing, Jiangsu, P.R. China.

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http://dx.doi.org/10.1016/j.gie.2020.09.032DOI Listing
January 2021

Hierarchical Multimodal Adaptive Fusion (HMAF) Network for Prediction of RGB-D Saliency.

Authors:
Ying Lv Wujie Zhou

Comput Intell Neurosci 2020 20;2020:8841681. Epub 2020 Nov 20.

School of Information and Electronic Engineering, Zhejiang University of Science & Technology, Hangzhou 310023, China.

Visual saliency prediction for RGB-D images is more challenging than that for their RGB counterparts. Additionally, very few investigations have been undertaken concerning RGB-D-saliency prediction. The proposed study presents a method based on a hierarchical multimodal adaptive fusion (HMAF) network to facilitate end-to-end prediction of RGB-D saliency. In the proposed method, hierarchical (multilevel) multimodal features are first extracted from an RGB image and depth map using a VGG-16-based two-stream network. Subsequently, the most significant hierarchical features of the said RGB image and depth map are predicted using three two-input attention modules. Furthermore, adaptive fusion of saliencies concerning the above-mentioned fused saliency features of different levels (hierarchical fusion saliency features) can be accomplished using a three-input attention module to facilitate high-accuracy RGB-D visual saliency prediction. Comparisons based on the application of the proposed HMAF-based approach against those of other state-of-the-art techniques on two challenging RGB-D datasets demonstrate that the proposed method outperforms other competing approaches consistently by a considerable margin.
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http://dx.doi.org/10.1155/2020/8841681DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7700038PMC
July 2021

A novel secretagogin/ATF4 pathway is involved in oxidized LDL-induced endoplasmic reticulum stress and islet β-cell apoptosis.

Acta Biochim Biophys Sin (Shanghai) 2021 Jan;53(1):54-62

Department of Metabolism & Endocrinology, The First Affiliated Hospital of the University of South China, Hengyang 421001, China.

Excessive accumulation of cholesterol in β cells initiates endoplasmic reticulum (ER) stress and associated apoptosis. We have reported that excessive uptake of cholesterol by MIN6 cells decreases the expression of secretagogin (SCGN) and then attenuates insulin secretion. Here, we aimed to determine whether cholesterol-induced SCGN decrease is involved in the modulation of ER stress and apoptosis in pancreatic β cells. In this study, MIN6 cells were treated with oxidized low-density lipoprotein (ox-LDL) for 24 h, and then intracellular lipid droplets and cell apoptosis were quantified, and SCGN and ER stress markers were identified by western blot analysis. Furthermore, small interfer RNA (siRNA)-mediated SCGN knockdown and recombinant plasmid-mediated SCGN restoration experiments were performed to confirm the role of SCGN in ER stress and associated cell apoptosis. Finally, the interaction of SCGN with ATF4 was computationally predicted and then validated by a co-immunoprecipitation assay. We found that ox-LDL treatment increased the levels of ER stress markers, such as phosphorylated protein kinase-like endoplasmic reticulum kinase, phosphorylated eukaryotic initiation factor 2 alpha, activating transcription factor 4 (ATF4), and transcription factor CCAAT-enhancer-binding protein homologous protein, and promoted MIN6 cell apoptosis; in addition, the expression of SCGN was downregulated. siRNA-mediated SCGN knockdown exacerbated β-cell ER stress by increasing ATF4 expression. Pretreatment of MIN6 cells with the recombinant SCGN partly antagonized ox-LDL-induced ER stress and apoptosis. Furthermore, a co-immunoprecipitation assay revealed an interaction between SCGN and ATF4 in MIN6 cells. Taken together, these results demonstrated that pancreatic β-cell apoptosis induced by ox-LDL treatment can be attributed, in part, to an SCGN/ATF4-dependent ER stress response.
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http://dx.doi.org/10.1093/abbs/gmaa142DOI Listing
January 2021

[Autophagy activation attenuates the neurotoxicity of local anaesthetics by decreasing caspase-3 activity in rats].

Braz J Anesthesiol 2020 Nov-Dec;70(6):627-634. Epub 2020 Nov 18.

The First Hospital of Lanzhou University, Department of Anaesthesiology, Lanzho, China.

Background And Objectives: The mechanisms by which local anaesthetics cause neurotoxicity are very complicated. Apoptosis and autophagy are highly coordinated mechanisms that maintain cellular homeostasis against stress. Studies have shown that autophagy activation serves as a protective mechanism in vitro. However, whether it also plays the same role in vivo is unclear. The aim of this study was to explore the role of autophagy in local anaesthetic-induced neurotoxicity and to elucidate the mechanism of neurotoxicity in an intrathecally injected rat model.

Methods: Eighteen healthy adult male Sprague-Dawley rats were randomly divided into three groups. Before receiving an intrathecal injection of 1% bupivacaine, each rat received an intraperitoneal injection of vehicle or rapamycin (1 mg.kg) once a day for 3 days. The pathological changes were examined by Haematoxylin and Eosin (HE) staining. Apoptosis was analysed by TdT-mediated dUTP Nick-End Labelling (TUNEL) staining. Caspase-3, Beclin1 and LC3 expression was examined by Immunohistochemical (IHC) staining. Beclin1 and LC3 expression and the LC3-II/LC3-I ratio were detected by western blot analysis.

Results: After bupivacaine was injected intrathecally, pathological damage occurred in spinal cord neurons, and the levels of apoptosis and caspase-3 increased. Enhancement of autophagy with rapamycin markedly alleviated the pathological changes and decreased the levels of apoptosis and caspase-3 while increasing the expression of LC3 and Beclin1 and the ratio of LC3-II to LC3-I.

Conclusions: Enhancement of autophagy decreases caspase-3-dependent apoptosis and improves neuronal survival in vivo. Activation of autophagy may be a potential therapeutic strategy for local anaesthetic-induced neurotoxicity.
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http://dx.doi.org/10.1016/j.bjan.2020.04.020DOI Listing
November 2020

Development and validation of a real-time artificial intelligence-assisted system for detecting early gastric cancer: A multicentre retrospective diagnostic study.

EBioMedicine 2020 Dec 27;62:103146. Epub 2020 Nov 27.

Department of Gastroenterology, Nanjing Drum Tower Hospital, Affiliated Drum Tower Hospital, Medical School of Nanjing University, 321 Zhongshan Road, Nanjing, Jiangsu 210008, China. Electronic address:

Background: We aimed to develop and validate a real-time deep convolutional neural networks (DCNNs) system for detecting early gastric cancer (EGC).

Methods: All 45,240 endoscopic images from 1364 patients were divided into a training dataset (35823 images from 1085 patients) and a validation dataset (9417 images from 279 patients). Another 1514 images from three other hospitals were used as external validation. We compared the diagnostic performance of the DCNN system with endoscopists, and then evaluated the performance of endoscopists with or without referring to the system. Thereafter, we evaluated the diagnostic ability of the DCNN system in video streams. The accuracy, sensitivity, specificity, positive predictive value, negative predictive value and Cohen's kappa coefficient were measured to assess the detection performance.

Finding: The DCNN system showed good performance in EGC detection in validation datasets, with accuracy (85.1%-91.2%), sensitivity (85.9%-95.5%), specificity (81.7%-90.3%), and AUC (0.887-0.940). The DCNN system showed better diagnostic performance than endoscopists and improved the performance of endoscopists. The DCNN system was able to process oesophagogastroduodenoscopy (OGD) video streams to detect EGC lesions in real time.

Interpretation: We developed a real-time DCNN system for EGC detection with high accuracy and stability. Multicentre prospective validation is needed to acquire high-level evidence for its clinical application.

Funding: This work was supported by the National Natural Science Foundation of China (grant nos. 81672935 and 81871947), Jiangsu Clinical Medical Center of Digestive System Diseases and Gastrointestinal Cancer (grant no. YXZXB2016002), and Nanjing Science and Technology Development Foundation (grant no. 2017sb332019).
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http://dx.doi.org/10.1016/j.ebiom.2020.103146DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7708824PMC
December 2020

The Removal of Platinum Group Metals, Cs, Se, and Te from Nuclear Waste Glass Using Liquid Sb Extraction and Phase Separation Methods.

Materials (Basel) 2020 Nov 23;13(22). Epub 2020 Nov 23.

Shaanxi Key Laboratory of Advanced Nuclear Energy and Technology, and Shaanxi Engineering Research Center of Advanced Nuclear Energy, School of Nuclear Science and Technology, Xi'an Jiaotong University, No. 28, Xianning West Road, Xi'an 710049, Shaanxi, China.

Recovery of platinum group metals (PGMs: Pd, Ru, Rh), Cs, Se, and Te from molten borosilicate glass containing simulated high level radwaste through the combination of liquid metal extraction and phase separation method under reductive heat-treatment was studied. In this process, the PGMs were extracted in recovered liquid metal phase, where Sb and Bi metals were used as the collecting metals. Meanwhile, Cs, Se, and Te were enriched in the phase separated potassium-rich materials on glass surface, which were extracted by water. The type of liquid metals had profound influence on the extraction behaviors of PGMs and other fission products from the glass melt. As a result, except the near extraction efficiency of Pd, Sb showed higher affinity for Ru and Rh than Bi metal. The higher phase separation efficiency of potassium-rich materials led to the higher extraction efficiencies of Cs, Se, and Te in liquid Sb extraction than Bi. Among the examined conditions, using liquid Sb extraction, the Pd, Ru, and Rh extraction efficiencies were 78.6%, 62.1% and 100% in liquid Sb metal phase, and 93.76% of Cs, 60.4% of Se, and 23.65% of Te in leachate were obtained.
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http://dx.doi.org/10.3390/ma13225305DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7700306PMC
November 2020
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