Publications by authors named "Ying Liu"

7,838 Publications

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One Health approach to improve the malaria elimination programme in Henan Province.

Adv Parasitol 2022 26;116:153-186. Epub 2022 Mar 26.

Henan Center for Disease Control and Prevention, Zhengzhou, Henan, PR China. Electronic address:

One Health is a collaborative, multi-sectoral, trans-disciplinary approach with the goal of achieving optimal health outcomes by recognizing the interconnection between people, animals, plants, and the environment and determining how this relates to the control of infectious diseases such as malaria, schistosomiasis and so on. Malarias caused by Plasmodium that commonly infects female Anopheles mosquitoes, which feed on human blood and act as a disease vector. It has been a worldwide important public health problem from ancient times. Also, malaria is one of the infectious diseases with the longest epidemic time and the most serious harm in the history of Henan Province, China. During the past decades, the multi-sectoral, cross-regional, and multi-disciplinary One Health approach contributed to a significant reduction in malaria incidence, resulting in initiation of the Henan Malaria Elimination Action Plan. Herein, we reviewed the history of the fight against malaria in Henan Province. A full picture of malaria epidemics, prevention, and control strategies were showed, with the objective that it will help stakeholders, and policy-makers to take informed decisions on public health issues and intervention designs on malaria control towards elimination in the similar areas.
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http://dx.doi.org/10.1016/bs.apar.2022.02.001DOI Listing
March 2022

Serum prolactin level as a predictive factor for abiraterone response in patients with metastatic castration-resistant prostate cancer.

Prostate 2022 Jun 23. Epub 2022 Jun 23.

Department of Urology, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.

Background: To investigate the prognostic value and potential therapeutic target of the baseline serum hormones in patients with metastatic castration-resistant prostate cancer (mCRPC) treated with abiraterone.

Methods: This retrospective study was performed in patients with mCRPC receiving abiraterone acetate (AA) from July 2016 to September 2020. Patients who had serum hormone tests within 2 weeks before AA treatment were included. Univariate analysis and Cox regression were performed to evaluate the correlation of sex hormones with progression-free survival (PFS) and overall survival (OS). Prolactin (PRL) expression in the clinical specimens was evaluated by immunohistochemistry. Bone metastases were quantified by automated Bone Scan Index (aBSI).

Results: The study included 61 patients with a median follow-up of 19.0 months. Patients with lower baseline PRL levels (median) responded better to AA than those with higher baseline PRL levels as indicated by prostate-specific antigen (PSA) reduction (PSA90, 66.7% vs. 25.8%, p = 0.001), PFS (19.6 vs. 7.9 months), and OS (52.8 vs. 19.2 months). Cox regression adjusted for clinical factors also confirmed that baseline PRL level was an independent predictive factor for PFS (hazard ratio = 1.096, p = 0.007). Prostatic PRL expression increased as the disease progressed. PRL expression was also detected in biopsy samples from bone metastasis but not in normal bone tissue, and the serum PRL levels were positively correlated with aBSIs (r = 0.28, p = 0.037).

Conclusions: Serum PRL levels are predictive of response to AA in patients with mCRPC. Serum PRL levels are positively correlated with the volume of metastatic bone disease.
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http://dx.doi.org/10.1002/pros.24402DOI Listing
June 2022

A New Low-Temperature Solder Assembly Technique to Replace Eutectic Sn-Bi Solder Assembly.

Micromachines (Basel) 2022 May 31;13(6). Epub 2022 May 31.

Department of Advanced Design and System Engineering, City University of Hong Kong, Hong Kong, China.

We successfully achieved low-temperature assembly by reflowing the 13.5Sn-37.5Bi-45In-4Pb quaternary eutectic solder paste and the SAC 305 solder ball together at 140 °C for 5 min. The wetting angle of the mixed solder joint is 17.55°. The overall atomic percent of Pb in the mixed solder joint is less than 1%, which can be further reduced or eliminated. Moreover, after aging at 80 °C for 25 days, we observed no obvious decrease in shear strength of the fully mixed solder joint, which is the most advantage of this assembly technique over Sn58Bi solder assembly. The Bi phase segregation at the interface is slowed down compared with Sn-Bi solder joint. This low-temperature assembly is promising to be applied in advanced packaging technology to replace the eutectic Sn-Bi solder.
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http://dx.doi.org/10.3390/mi13060867DOI Listing
May 2022

Transcriptomic Profile Analysis of × Response and Tolerance under Salt Stress Conditions.

Genes (Basel) 2022 Jun 8;13(6). Epub 2022 Jun 8.

College of Life Science and Technology, Tarim University, Alar 843300, China.

A new variety with a strong salt tolerance was obtained from cross breeding as the female parent and as the male parent. In order to elucidate the molecular mechanism and find out the major differentially expressed genes of salt tolerance of , after being subjected to salt stress, at 0, 200, and 400 mmol/L NaCl, the root, stem, and leaf transcriptomes (denoted as R0, S0, and L0; R200, S200, and L200; and R400, S400, and L400, respectively) of × were sequenced. In total, 41,617 differentially expressed genes (DEGs) were identified in all the comparison groups with 21,603 differentially upregulated genes and 20,014 differentially downregulated genes. Gene Ontology analysis showed that DEGs were significantly enriched in biological processes that may be involved in salt stress, such as 'cell communication', 'ion transport', 'signaling', and signal 'transmission'. Kyoto Encyclopedia of Genes and Genomes analysis showed that DEGs were mainly enriched in pathways of 'plant-pathogen interaction', 'carbon metabolism', and 'plant hormone signal transmission'. The pathways and related gene information formed a basis for future research on the mechanisms of salt stress, the development of molecular markers, and the cloning of key genes in × .
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http://dx.doi.org/10.3390/genes13061032DOI Listing
June 2022

RNA m6A Modification Changes in Postmortem Nucleus Accumbens of Subjects with Alcohol Use Disorder: A Pilot Study.

Genes (Basel) 2022 May 27;13(6). Epub 2022 May 27.

Department of Psychiatry, Boston University School of Medicine, Boston, MA 02118, USA.

Background: The nucleus accumbens (NAc) is a key brain structure mediating the rewarding effect of alcohol and drug abuse. Chronic alcohol consumption may alter RNA methylome (or epitranscriptome) in the NAc, leading to altered gene expression and thus behavioral neuroadaptation to alcohol.

Methods: This pilot study profiled the epitranscriptomes of mRNAs, long noncoding RNAs (lncRNAs), and microRNAs (miRNAs) in postmortem NAc of three male Caucasian subjects with alcohol use disorder (AUD) and three matched male Caucasian control subjects using Arraystar's m6A-mRNA&lncRNA Epitranscriptomic Microarray assay. Differentially methylated (DM) RNAs and the function of DM RNAs were analyzed by biostatistics and bioinformatics programs.

Results: 26 mRNAs were hypermethylated and three mRNAs were hypomethylated in the NAc of AUD subjects (≥2-fold changes and ≤ 0.05). Most of these 29 DM mRNAs are involved in immune-related pathways (e.g., IL-17 signaling). Moreover, four lncRNAs were hypermethylated and one lncRNA was hypomethylated in the NAc of AUD subjects (≥2-fold changes and ≤ 0.05). Additionally, three miRNAs were hypermethylated in the NAc of AUD subjects (≥2-fold changes and ≤ 0.05).

Conclusions: This study revealed RNA methylomic changes in the NAc of AUD subjects, suggesting that chronic alcohol consumption may lead to AUD through epitranscriptomic RNA modifications. Our findings need to be replicated in a larger sample.
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http://dx.doi.org/10.3390/genes13060958DOI Listing
May 2022

Autophagy triggered by the ROS/ERK signaling pathway protects mouse embryonic palatal cells from apoptosis induced by nicotine.

Environ Sci Pollut Res Int 2022 Jun 23. Epub 2022 Jun 23.

Laboratory of Orofacial Development, Laboratory of Molecular Signaling and Stem Cells Therapy, Molecular Laboratory for Gene Therapy and Tooth Regeneration, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Beijing, 100050, China.

Maternal cigarette smoking during pregnancy is a known high-risk factor for having a child with a cleft lip and/or palate (CLP), a common congenital malformation. Nicotine is the major teratogen component of cigarettes and e-cigarettes, and nicotine plays an important role in the development of CLP. However, the mechanism underlying nicotine's effect on CLP remains unclear. Here, we aimed to determine the role and molecular mechanisms of nicotine-induced autophagy, an important process involved in regulating the cellular stress response in mouse embryonic palatal cells (MEPCs). First, we found that nicotine promoted MEPCs proliferation and inhibited their apoptosis from 0 to 12 h. After 12 h, the proliferation was inhibited, and apoptosis was promoted. The migration of MEPCs was also inhibited by nicotine. Simultaneously, long-term nicotine stimulation inhibited the osteogenic differentiation of MEPCs. We then found that nicotine significantly increased autophagy flux in MEPCs at 12 h by increasing the expression of microtubule-associated protein light chain 3 (LC3) and reducing P62 expression levels. After nicotine exposure, intracellular reactive oxygen species (ROS) and extracellular signal-regulated kinase-1/2 (ERK1/2) expression significantly increased, and the expression of ERK1/2 was reversed by the ROS scavenging agent N-acetylcysteine (NAC). Moreover, the autophagy induced by nicotine was reversed by SCH772984, a specific inhibitor of ERK1/2, and the autophagy inhibitor chloroquine (CQ). These results suggest that in the early stage of nicotine exposure, MEPCs may trigger autophagy through the ROS/ERK1/2 signaling pathway to avoid cell damage caused by nicotine.
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http://dx.doi.org/10.1007/s11356-022-21496-0DOI Listing
June 2022

LC-MS/MS assay of fluoropezil and its two major metabolites in human plasma: an application to pharmacokinetic studies.

Bioanalysis 2022 Jun 23. Epub 2022 Jun 23.

Shanghai Institute of Materia Medica, Chinese Academy of Sciences, No. 501 Haike Road & No. 555 Zuchongzhi Road, Shanghai, 201203, PR China.

LC-MS/MS methods were developed for pharmacokinetic analysis and verified to measure fluoropezil, a new AchE inhibitor for Alzheimer's disease treatment, and its two primary metabolites (-debenzyl fluoride fluoropezil [M1] and -oxidized fluoropezil [M11]) in human plasma. Analytes were extracted from 50 μl plasma using protein precipitation and separated by HPLC using a bridged ethyl hybrid column and gradient elution procedure. Analytical detection was performed with a triple quadrupole mass spectrometer and electrospray ionization source in multiple reaction monitoring mode. The LC-MS/MS method was fully validated. The quantification linear ranges were 0.100-50.0 ng/ml (fluoropezil), 0.0500-25.0 ng/ml (M1) and 0.0500-25.0 ng/ml (M11). A sensitive, reliable LC-MS/MS method was established and used successfully to explore the pharmacokinetics of fluoropezil.
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http://dx.doi.org/10.4155/bio-2022-0045DOI Listing
June 2022

Comment on: Treatment efficacy for rectal Neisseria gonorrhoeae: a systematic review and meta-analysis of randomized controlled trials.

J Antimicrob Chemother 2022 Jun 23. Epub 2022 Jun 23.

Emergency Department, Wan Fang Hospital, Taipei Medical University, Taipei, Taiwan.

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http://dx.doi.org/10.1093/jac/dkac194DOI Listing
June 2022

Genetic polymorphisms in DNA repair genes and their association with risk of cervical cancer: A systematic review and meta-analysis.

J Obstet Gynaecol Res 2022 Jun 22. Epub 2022 Jun 22.

Institute of Traditional Chinese Medicine Literature, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.

Background: There have been a large number of epidemiologic studies regarding the association between genetic polymorphisms in DNA repair genes and onset of cervical cancer. However, results are inconsistent.

Methods: Articles published before June 2021 and regarding genetic polymorphisms in DNA repair genes and cervical cancer were searched in following databases: PubMed, Web of Science, Google Scholar, and CNKI. With at least three articles for each polymorphism, we made meta-analysis to compute multivariate odds ratios (ORs) and their 95% confidence intervals (CIs).

Results: The present study showed significant associations between XRCC1 Arg399Gln polymorphisms and risk of cervical cancer in Asian, whereas no significant association between them were showed in Caucasian (Asian: GA vs. GG: OR = 1.27, 95%CI 1.06-1.52; AA vs. GG: OR = 1.91, 95%CI 1.29-2.83; GA + AA vs. GG: OR = 1.36, 95%CI 1.12-1.65; AA vs. GG + GA: OR = 1.66, 95%CI 1.17-2.37; Caucasian: GA vs. GG: OR = 1.08, 95%CI 0.83-1.41; AA vs. GG: OR = 2.18, 95%CI 0.75-6.31; GA + AA vs. GG: OR = 1.23, 95%CI 0.85-1.78; AA vs. GG + GA: OR = 1.70, 95%CI 0.69-4.18). In addition, there were significant associations between ERCC2 rs13181 polymorphisms and risk of cervical cancer in Asian (AC vs AA: OR = 0.53, 95%CI 0.37-0.75, I  = 0.0%, p value of Q test = 0.847; AC + CC vs AA: OR = 0.50, 95%CI 0.36-0.70, I  = 0.0%, p value of Q test = 0.856).

Conclusions: The meta-analysis showed that there were significant associations between XRCC1 Arg399Gln and ERCC2 rs13181 polymorphisms and risk of cervical cancer.
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http://dx.doi.org/10.1111/jog.15325DOI Listing
June 2022

DNA Origami-Platelet Adducts: Nanoconstruct Binding without Platelet Activation.

Bioconjug Chem 2022 Jun 22. Epub 2022 Jun 22.

Department of Mechanical Engineering, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, United States.

. Platelets are small, mechanosensitive blood cells responsible for maintaining vascular integrity and activatable on demand to limit bleeding and facilitate thrombosis. While circulating in the blood, platelets are exposed to a range of mechanical and chemical stimuli, with the platelet membrane being the primary interface and transducer of outside-in signaling. Sensing and modulating these interface signals would be useful to study mechanochemical interactions; yet, to date, no methods have been defined to attach adducts for sensor fabrication to platelets without triggering platelet activation. We hypothesized that DNA origami, and methods for its attachment, could be optimized to enable nonactivating instrumentation of the platelet membrane. . We designed and fabricated multivalent DNA origami nanotile constructs to investigate nanotile hybridization to membrane-embedded single-stranded DNA-tetraethylene glycol cholesteryl linkers. Two hybridization protocols were developed and validated (Methods I and II) for rendering high-density binding of DNA origami nanotiles to human platelets. Using quantitative flow cytometry, we showed that DNA origami binding efficacy was significantly improved when the number of binding overhangs was increased from two to six. However, no additional binding benefit was observed when increasing the number of nanotile overhangs further to 12. Using flow cytometry and transmission electron microscopy, we verified that hybridization with DNA origami constructs did not cause alterations in the platelet morphology, activation, aggregation, or generation of platelet-derived microparticles. . Herein, we demonstrate that platelets can be successfully instrumented with DNA origami constructs with no or minimal effect on the platelet morphology and function. Our protocol allows for efficient high-density binding of DNA origami to platelets using low quantities of the DNA material to label a large number of platelets in a timely manner. Nonactivating platelet-nanotile adducts afford a path for advancing the development of DNA origami nanoconstructs for cell-adherent mechanosensing and therapeutic agent delivery.
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http://dx.doi.org/10.1021/acs.bioconjchem.2c00197DOI Listing
June 2022

[Corrigendum] BMI‑1 promotes invasion and metastasis in endometrial adenocarcinoma and is a poor prognostic factor.

Oncol Rep 2022 Aug 22;48(2). Epub 2022 Jun 22.

Department of Obstetrics and Gynecology, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, Shandong 264100, P.R. China.

Following the publication of the above paper, an interested reader drew to the authors' attention that, in Fig. 5 on p. 1637, the data panel selected to represent the 'Ishikawa/+BMI‑1 siRNA/Invasion' experiment in Fig. 5A appeared to overlap with the data shown for the 'JEC/+BMI‑1 siRNA/Migration' panel in Fig. 5C, such that they were potentially derived from the same original source. The authors have re‑examined their original data, and realize that the data panel selected for the 'Ishikawa/+BMI‑1 siRNA/Invasion' experiment in Fig. 5A was inadvertently selected incorrectly. The corrected version of Fig. 5, showing the correct data for the 'Ishikawa/+BMI‑1 siRNA/Invasion' experiment in Fig. 5A, is shown below. Note that this error did not affect the overall conclusions reported in the study. The authors are grateful to the Editor of for granting them the opportunity to publish this Corrigendum; furthermore, they apologize for any inconvenience caused to the readership of the Journal. [Oncology Reports 43: 1630‑1640, 2020; DOI: 10.3892/or.2020.7539].
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http://dx.doi.org/10.3892/or.2022.8352DOI Listing
August 2022

The clinical and pathological relevance of waxy casts in urine sediment.

Ren Fail 2022 Dec;44(1):1038-1044

Renal Division, Peking University First Hospital, Institute of Nephrology, Peking University, Beijing, China.

Although casts in urine may imply the underlying pathogenesis and the diagnosis, the waxy cast is poorly understood yet. We aim to investigate the association between waxy casts and clinicopathological indices. Patients undergone renal biopsy and urine sediment examination were enrolled. Waxy casts referred to those presented with a homogeneous melted wax appearance and pre-waxy casts referred to those in which one or more segments demonstrated a waxy-cast appearance. Multivariable logistic regression was used to assess the factors associated with waxy casts. In 1282 patients, the detection rate of waxy casts was 26.3%. If either waxy or pre-waxy cast was considered as a diagnostic marker for renal insufficiency (eGFR < 60 ml/min/1.73 m), the sensitivity was 0.58 and the specificity was 0.88. If the only waxy cast was considered as the diagnostic marker, the sensitivity was 0.29 and the specificity was 0.97. The patients with waxy or pre-waxy casts had higher blood pressure, more proteinuria, and worse renal function. Waxy or pre-waxy cast was independently associated with eGFR (odds ratio: 0.73 per 10 mL/min/1.73 m increase, 95% confidence interval: 0.69-0.77,  < 0.001), proteinuria (odds ratio: 1.07 per 1 g/day increase, 95% confidence interval: 1.03-1.10,  < 0.001) and pathological lesions. Waxy or pre-waxy casts are closely related to impaired renal function. Their presence is a specific indicator of renal insufficiency but is not sensitive enough.
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http://dx.doi.org/10.1080/0886022X.2022.2088388DOI Listing
December 2022

Endovascular thrombectomy with or without intravenous alteplase for acute ischemic stroke due to large vessel occlusion: a systematic review and meta-analysis of randomized trials.

Stroke Vasc Neurol 2022 Jun 20. Epub 2022 Jun 20.

Department of Pharmacy, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China

Background: Among patients who had an ischaemic stroke presenting directly to a stroke centre where endovascular thrombectomy (EVT) is immediately available, there is uncertainty regarding the role of intravenous thrombolysis agents before or concurrently with EVT. To support a rapid guideline, we conducted a systematic review and meta-analysis to examine the impact of EVT alone versus EVT with intravenous alteplase in patients who had an acute ischaemic stroke due to large vessel occlusion.

Methods: In November 2021, we searched MEDLINE, Embase, PubMed, Cochrane, Web of Science, clincialtrials.gov and the ISRCTN registry for randomised controlled trials (RCTs) comparing EVT alone versus EVT with alteplase for acute ischaemic stroke. We conducted meta-analyses using fixed effects models and assessed the certainty of evidence using the GRADE approach.

Results: In total 6 RCTs including 2334 participants were eligible. Low certainty evidence suggests that, compared with EVT and alteplase, there is possibly a small decrease in the proportion of patients independent with EVT alone (risk ratio (RR) 0.97, 95% CI 0.89 to 1.05; risk difference (RD) -1.5%; 95% CI -5.4% to 2.5%), and possibly a small increase in mortality with EVT alone (RR 1.07, 95% CI 0.88 to 1.29; RD 1.2%, 95% CI -2.0% to 4.9%) . Moderate certainty evidence suggests that there is probably a small decrease in symptomatic intracranial haemorrhage (sICH) with EVT alone (RR 0.75, 95% CI 0.52 to 1.07; RD -1.0%; 95%CI -1.8% to 0.27%).

Conclusions: Low certainty evidence suggests that there is possibly a small decrease in the proportion of patients that achieve functional independence and a small increase in mortality with EVT alone. Moderate certainty evidence suggests that there is probably a small decrease in sICH with EVT alone. The accompanying guideline provides contextualised guidance based on this body of evidence.

Prospero Registration Number: CRD42021249873.
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http://dx.doi.org/10.1136/svn-2022-001547DOI Listing
June 2022

Glucose-responsive biomimetic nanoreactor in bacterial cellulose hydrogel for antibacterial and hemostatic therapies.

Carbohydr Polym 2022 Sep 13;292:119615. Epub 2022 May 13.

Laboratory of Eco-textiles of Ministry of Education, College of Textile Science and Engineering, Jiangnan University, 214122 Wuxi, Jiangsu, PR China. Electronic address:

Anti-infections therapy accompanied with effective hemorrhage control is highly urgent in clinics. Herein, a biomimetic nanoreactor encapsulated self-healing hydrogel with glucose-responsive catalytic activity was constructed for synergetic antibacterial defense and hemostasis. A metal-organic frameworks (MOF)-based nanocatalyst loaded with glucose oxidase (GOx) was fabricated and encapsulated in the bacterial cellulose (BC) reinforced hydrogel, while the sustained release of GOx could catalyze the decomposition of glucose for triggering the MOF-mediated catalytic activity to in situ generate OH for bacteria killing. Importantly, this nanozyme-based hydrogel exhibited excellent hemostatic property owing to the enhanced absorption capacity, which provides the essential glucose from blood for triggering the glucose-responsive antibacterial activity simultaneously. Antibacterial tests revealed that the hydrogel exhibited significant antibacterial efficacy and biofilm inhibition ability in the presence of normal blood level of glucose. Such design of biocatalytic hydrogel for synergistic hemostatic and antibacterial application brings new insight for nanozyme-based biomedical therapies.
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http://dx.doi.org/10.1016/j.carbpol.2022.119615DOI Listing
September 2022

mutation landscape in non-small cell lung cancer patients in Northeast China.

Tumori 2022 Jun 19:3008916221101426. Epub 2022 Jun 19.

Translational Oncology Research Lab, Jilin Cancer Hospital, Changchun, China.

Objective: This study aimed to explore erb-b2 receptor tyrosine kinase 2 () gene mutations in patients with non-small cell lung cancers (NSCLC) in Northeast China, and to analyze mutation subtypes and clinicopathological characteristics related to the presence of mutations.

Methods: In this study, 1087 tissue samples, 368 whole blood samples, and 68 pleural effusion samples from 1349 NSCLC were collected. Next-generation sequencing (NGS) was used to perform genetic testing on the samples. The proportion of patients with mutations and related clinicopathological characteristics were analyzed.

Results: The mutation rate of in NSCLC was 5.58% (85/1523). Of the patients with mutations, 27.63% (21/76) were over 65 years old, 59.21% (45/76) were women, and 68.42% (52/76) were non-smokers. The majority of tumors were adenocarcinomas (92.1%, 70/76) and stage III and IV diseases accounted for 81.58% (62/76) of all cases. There were 14 subtypes of mutations; the most frequently seen were copy number alteration (41.76%, 38/91) and exon 20 in-frame insertion (36.26%, 33/91). Of the patients with mutations, 24 had concurrent epidermal growth factor receptor mutations, seven had mesenchymal epithelial transition factor amplifications, and three had anaplastic lymphoma kinase mutations. The agreement between tissue and paired blood samples in the presence of mutations was 64.3% (9/14).

Conclusion: mutations in Northeast China NSCLC patients have a unique molecular spectrum. Our work can provide guidance for the clinical diagnosis and treatment of patients with mutations in Northeast China.
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http://dx.doi.org/10.1177/03008916221101426DOI Listing
June 2022

Study on the intervention effect and mechanism of bacillus Calmette-Guerin polysaccharide and nucleic acid injection on atopic dermatitis by targeting the transient receptor potential vanilloid subtype 1 pathway.

Ann Transl Med 2022 May;10(10):608

Department of Dermatology, The First Affiliated Hospital of Qiqihar Medical University, Qiqihar, China.

Background: This study aimed to explore the mechanism of Bacillus Calmette-Guerin polysaccharide and nucleic acid injection (BCG-PSN) targeting the transient receptor potential vanilloid subtype 1 (TRPV1) pathway for atopic dermatitis (AD) in mice.

Methods: Experiment 1: a total of 30 Kunming (KM) mice were randomized into blank control, model, BCG-PSN low-dose (25 g/kg), BCG-PSN medium-dose (75 g/kg), BCG-PSN high-dose (225 g/kg), and positive drug (hydrocortisone 25 mg/kg) control groups. The AD model mice were established by induction with 2,4-Dinitrochlorobenzene (DNCB). After treatment in groups, the symptom score and scratching frequency in skin lesions were observed. The levels of immunoglobulin E (IgE), interleukin (IL)-4, IL-31, and IL-13 in serum were detected, as well as the levels of tumor necrosis factor-α (TNF-α), TRPV1, and nuclear factor (NF)-κB p65 in skin lesions in each group. Experiment 2: the optimal dose of BCG-PSN in Experiment 1 was adopted. A total of 20 KM mice were randomized into blank control, model, BCG-PSN, and BCG-PSN + PAC (PAC-14028) groups. The symptom score and scratching frequency in skin lesions were observed. The levels of IgE, IL-4, IL-31, and IL-13 in serum were detected, as well as the levels of TNF-α and TRPV1 in skin lesions in each group.

Results: In Experiment 1, compared with the blank control group, the ear tissues of mice in model groups developed AD, with increased symptom score, scratching frequency, levels of IgE, IL-4, IL-31, and IL-13 in serum and levels of TNF-α, TRPV1, and NF-κB p65 in skin lesions. Compared with the model group, BCG-PSN low-dose, BCG-PSN medium-dose, BCG-PSN high-dose, and positive drug control groups had reduced AD symptoms, decreased symptom score, and decreased scratching frequency, with declined expression of each inflammatory substance, including the greatest decrease in the medium-dose group. In Experiment 2, after BCG-PSN was combined with PAC, the inflammation indexes decreased compared with those in the model group, and increased compared with those in the BCG-PSN group.

Conclusions: Intramuscular BCG-PSN can target the TRPV1 pathway, inhibit inflammation, and improve the symptoms of AD mice.
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http://dx.doi.org/10.21037/atm-22-2101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201144PMC
May 2022

Mutation in Enhanced the Adaptation of to Long-Term Acid Stress.

Front Microbiol 2022 26;13:829719. Epub 2022 May 26.

College of Plant Protection, Southwest University, Chongqing, China.

Bacterial wilt, caused by the plant pathogen , occurs more severely in acidified soil according to previous reports. However, cannot grow well in acidic environments under barren nutrient culture conditions, especially when the pH is lower than 5. With the worsening acidification of farmland, further determination of how adapts to the long-term acidic environment is worthwhile. In this study, experimental evolution was applied to evaluate the adaptability and mechanism of the experimental population responding to long-term acid stress. We chose the CQPS-1 strain as the ancestor, and minimal medium (MM medium) with different pH values as the culture environment to simulate poor soil. After 1500 generations of serial passage experiments in pH 4.9 MM, acid-adapted experimental strains (denoted as C49 strains) were obtained, showing significantly higher growth rates than the growth rates of control experimental strains (serial passage experiment in pH 6.5 MM, denoted as C65 strains). Competition experiments showed that the competitive indices (CIs) of all selected clones from C49 strains were superior to the ancestor in acidic environment competitiveness. Based on the genome variation analysis and functional verification, we confirmed that loss of function in the gene was associated with the acid fitness gain of , which meant that the inactivation of the PhcA regulator caused by gene mutation mediated the population expansion of when growing in an acidic stress environment. Moreover, the swimming motility of acid evolution strains and the deletion mutant was significantly enhanced compared to CQPS-1. This work provided evidence for understanding the adaptive strategy of to the long-term acidic environment.
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http://dx.doi.org/10.3389/fmicb.2022.829719DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204249PMC
May 2022

Case Report: Multimodal Imaging Guides the Management of an Eosinophilic Leukemia Patient With Eosinophilic Myocarditis and Intracardiac Thrombus.

Front Cardiovasc Med 2022 3;9:903323. Epub 2022 Jun 3.

Department of Cardiology, First Affiliated Hospital of Dalian Medical University, Dalian, China.

Background: Eosinophilic leukemia (EL) is a rare, serious and potentially life-threatening condition characterized by the overproduction of eosinophils leading to tissue eosinophilic infiltration and damage. Although multiple organ systems may be involved, progressive eosinophilic myocarditis (EM) is the most common cause of morbidity and mortality. Early diagnosis and follow-up surveillance combined with multimodal imaging are crucial for appropriate treatment of EM.

Case Summary: It's a rare case of EL with EM and intracardiac thrombus in a 59-year-old patient who presented with asthenia for 3 weeks. Full blood count analysis indicated significant eosinophilia. Bone marrow aspirate revealed dysplastic eosinophilia and a FIP1L1-PDGFRA fusion gene (4q12) was detected, confirming EL. Echocardiography revealed EM with intracardiac thrombus. This was later confirmed by cardiac magnetic resonance imaging. The patient was commenced on imatinib and prednisolone and good clinical response was obtained. Through 18F-FAPI PET/CT imaging, we obtained visualization of fibroblast activation changes in the early stage of cardiac structure remodeling. With anti-fibrotic therapy after heart failure, the patient achieved a good clinical response.

Conclusion: This case demonstrates visualization of fibroblast activation after EM. Multimodality imaging can provide early diagnosis and may guide tailored antifibrotic therapy in early stage of EM.
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http://dx.doi.org/10.3389/fcvm.2022.903323DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204136PMC
June 2022

15-Hydroxyprostaglandin Dehydrogenase Is a Predictor of Stroke-Associated Pneumonia.

Front Neurol 2022 26;13:893624. Epub 2022 May 26.

Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha, China.

Background And Purpose: Stroke is a serious fatal and disabling disease. Stroke-associated pneumonia (SAP) is the most common complication of stroke, which may further aggravate the stroke. The prevention and early prediction of SAP is a key clinical strategy. 15-hydroxyprostaglandin dehydrogenase (15-PGDH) is involved in pneumonia, while its relationship with SAP has yet to be determined. Therefore, we investigated the predictive value of 15-PGDH for SAP and visualized their relationship.

Methods: Stroke patients were recruited and divided into SAP group and Non-SAP group. Baseline demographic and clinical data were obtained from the medical record system, blood samples were collected to detect relevant variables and 15-PGDH levels. Patient characteristics were compared with a t-test. Binary logistic regression analysis was performed to determine the predictive value of 15-PGDH for SAP. Restricted cubic splines (RCS) were performed to visualize the relationship between 15-PGDH and SAP risk. Finally, the SAP patient characteristics between the severe group and mild group were compared.

Results: 50 patients were enrolled and divided into SAP group ( = 26) and Non-SAP group ( = 24). 15-PGDH in the SAP group was lower than that in the Non-SAP group (0.258 ± 0.275 vs. 0.784 ± 0.615, = 0.025). Binary logistic regression analysis revealed that the lower 15-PGDH, the higher the risk of SAP (OR = 0.04, 95%CI, 0.010-0.157, < 0.001). The RCS model showed the L-shaped relationship between 15-PGDH and SAP.

Conclusions: In stroke patients, serum 15-PGDH is a valuable biomarker for predicting SAP. There is an L-shaped relationship between the level of 15-PGDH and the risk of SAP.
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http://dx.doi.org/10.3389/fneur.2022.893624DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202497PMC
May 2022

Benefit Modeling and Analysis of Wind Power Generation under Social Energy Economy and Public Health.

J Environ Public Health 2022 9;2022:5635853. Epub 2022 Jun 9.

Center for Sustainable and Inclusive Development Studies (SID), Faculty of Economics and Management, Universiti Kebangsaan Malaysia (UKM), Bangi 43600, Selangor, Malaysia.

Wind is a renewable energy source. Overall, using wind to produce energy has fewer effects on the environment than many other energy sources. Wind and solar energy provide public health and environmental benefits to the social. Wind turbines may also reduce the amount of electricity generation from fossil fuels, which results in lower total air pollution and carbon dioxide emissions. In order to better optimize the effect of social energy economic management and facilitate the multiobjective decision making of coordinated development of energy and socioeconomic environment, a modeling and analysis method of economic benefits of wind power generation based on deep learning is proposed. In this paper, based on the principle of deep learning, the evaluation indicators of wind power economic benefits are excavated, a scientific and reasonable economic benefit evaluation system is constructed, a wind power economic benefit analysis model supported by public management is constructed, and the steps of wind power economic benefit analysis are simplified. It is concluded that the modeling and analysis method of wind power economic benefits based on deep learning has high practicability in the actual application process, which is convenient for the prediction and analysis of energy demand for social and economic development.
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http://dx.doi.org/10.1155/2022/5635853DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9203199PMC
June 2022

Mitogen-activated protein kinase TaMPK3 suppresses ABA response by destabilizing TaPYL4 receptor in wheat.

New Phytol 2022 Jun 20. Epub 2022 Jun 20.

Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS)/National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing, 100081, China.

Abscisic acid (ABA) receptors are considered as the targeted manipulation of ABA sensitivity and water productivity in plants. Regulation of their stability or activity will directly affect ABA signaling. Mitogen-activated protein kinase (MAPK) cascades link multiple environmental and plant developmental cues. However, the molecular mechanism of ABA signaling and MAPK cascade interaction remains largely elusive. TaMPK3 overexpression decreases drought tolerance and wheat sensitivity to ABA, significantly weakening ABA's inhibitory effects on growth. Under drought stress, overexpression lines show lower survival rates, shoot fresh weight, and proline content, but higher malondialdehyde (MDA) levels at seedling stage, as well as decreased grain width and thousand grain weight in both greenhouse and field conditions at the adult stage. TaMPK3-RNAi increases drought tolerance. TaMPK3 interaction with TaPYL4 leads to decreased TaPYL4 levels by promoting its ubiquitin-mediated degradation, while ABA treatment diminishes TaMPK3-TaPYL interactions. In addition, the expression of ABA signaling proteins is impaired in TaMPK3-overexpressing wheat plants under ABA treatment. The MPK3-PYL interaction module was found to be conserved across monocots and dicots. Our results suggest that the MPK3-PYL module could serve as a negative regulatory mechanism for balancing appropriate drought stress response with normal plant growth signaling in wheat.
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http://dx.doi.org/10.1111/nph.18326DOI Listing
June 2022

[HPLC fingerprint and content determination of three principal components in seeds of Crataegus pinnatifida].

Zhongguo Zhong Yao Za Zhi 2022 Jun;47(11):3000-3006

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.

The purpose of this paper is to establish the HPLC fingerprint and content determination method for Crataegus pinnatifida seeds. The separation was developed on a Welch Ultimate XB C_(18) column(4.6 mm×250 mm, 5 μm) by gradient elution with acetonitrile-water containing 0.1% formic acid as the mobile phase at the flow rate of 1 mL·min~(-1), the column temperature of 30 ℃, the injection volume of 10 μL, and the detection wavelength of 320 nm. Eighteen batches of samples were analyzed under the above chromatographic conditions to establish the fingerprint of C. pinnatifida seeds from different producing areas and a total of 24 common peaks were selected. The structures of three main chromatographic peaks were identified by comparison to reference substances, and the three compounds were simultaneously analyzed for content determination. They were identified as erythro-(7S,8R)-guaiacylglycerol-β-coniferyl aldehyde ether, threo-(7R,8R)-guaiacylglycerol-β-coniferyl aldehyde ether, and balanophonin, respectively. The relative similarity of fingerprints of 18 batches of samples and references ranged from 0.928 to 0.999, and the content of the three compounds was 0.055 1-0.182 7, 0.061 8-0.225 8, and 0.156 8-0.405 6 mg·g~(-1), respectively. SPSS 17.0 and SIMCA 14.1 were used for cluster analysis, principal component analysis(PCA), and orthogonal partial least squares discriminant analysis(OPLS-DA) on the common peaks of the HPLC fingerprint of C. pinnatifida seeds. The results showed that there were significant differences between the two batches of samples from Liaoning province and the other samples, and the three compounds to be tested were the main components leading to the difference of C. pinnatifida seeds. The established method was simple and reliable and can be used for qualitative and quantitative analysis of C. pinnatifida seeds. The findings of this study are expected to provide a scientific basis for quality control of C. pinnatifida seeds.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20211227.202DOI Listing
June 2022

The first high-quality reference genome of sika deer provides insights for high-tannin adaptation.

Genomics Proteomics Bioinformatics 2022 Jun 16. Epub 2022 Jun 16.

Key Laboratory of Genetics, Breeding and Reproduction of Special Economic Animals, Ministry of Agriculture and Rural Affairs, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China.

Sika deer are known to prefer oak leaves, which are rich in tannins and toxic to most mammals; however, the genetic mechanisms underlying their unique ability to adapt to living in the jungle are still unclear. In identifying the mechanism responsible for the tolerance of a highly toxic diet, we have made a major advancement by explaining the genomics of sika deer. We generated the first high-quality, chromosome-level genome assembly of sika deer and measured the correlation between tannin intake and RNA expression in 15 tissues through 180 experiments. Comparative genome analyses showed that the UGT and CYP gene families are functionally involved in the adaptation of sika deer to high-tannin food, especially the expansion of the UGT family 2 subfamily B of UGT genes. The first chromosome-level assembly and genetic characterization of the tolerance to a highly toxic diet suggest that the sika deer genome may serve as an essential resource for understanding evolutionary events and tannin adaptation. Our study provides a paradigm of comparative expressive genomics that can be applied to the study of unique biological features in non-model animals.
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http://dx.doi.org/10.1016/j.gpb.2022.05.008DOI Listing
June 2022

Retraction Note to: LncRNA BCRT1 promotes breast cancer progression by targeting miR-1303/PTBP3 axis.

Mol Cancer 2022 Jun 17;21(1):131. Epub 2022 Jun 17.

Department of Breast Surgery, Qilu Hospital of Shandong University, Jinan, Shandong, 250012, People's Republic of China.

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http://dx.doi.org/10.1186/s12943-022-01576-yDOI Listing
June 2022

Astragalus polysaccharide: a review of its immunomodulatory effect.

Arch Pharm Res 2022 Jun 17. Epub 2022 Jun 17.

Department of Anorectal Surgery, Third People's Hospital of Chengdu, Chengdu, China.

The Astragalus polysaccharide is an important bioactive component derived from the dry root of Astragalus membranaceus. This review aims to provide a comprehensive overview of the research progress on the immunomodulatory effect of Astragalus polysaccharide and provide valuable reference information. We review the immunomodulatory effect of Astragalus polysaccharide on central and peripheral immune organs, including bone marrow, thymus, lymph nodes, spleen, and mucosal tissues. Furthermore, the immunomodulatory effect of Astragalus polysaccharide on a variety of immune cells is summarized. Studies have shown that Astragalus polysaccharide can promote the activities of macrophages, natural killer cells, dendritic cells, T lymphocytes, B lymphocytes and microglia and induce the expression of a variety of cytokines and chemokines. The immunomodulatory effect of Astragalus polysaccharide makes it promising for the treatment of many diseases, including cancer, infection, type 1 diabetes, asthma, and autoimmune disease. Among them, the anticancer effect is the most prominent. In short, Astragalus polysaccharide is a valuable immunomodulatory medicine, but further high-quality studies are warranted to corroborate its clinical efficacy.
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http://dx.doi.org/10.1007/s12272-022-01393-3DOI Listing
June 2022

Qualitative and quantitative determination of the primary active components and metabolites in human plasma after oral administration of Shuanghuanglian liquid.

J Sep Sci 2022 Jun 17. Epub 2022 Jun 17.

Shanghai Institute of Materia Medica, Chinese Academy of Sciences, No.501 Haike Road, Shanghai, 201203, P.R. China.

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http://dx.doi.org/10.1002/jssc.202200121DOI Listing
June 2022

Self-assembled cupric oxide nanoclusters for highly efficient chemodynamic therapy.

Chem Asian J 2022 Jun 17. Epub 2022 Jun 17.

Nanjing University, Department of Chemistry, Xian Lin Da Dao 163#, 210023, Nanjing, CHINA.

Chemodynamic Therapy (CDT) that based on Fenton and Fenton-like reaction induce cancer cell killing via in situ catalyzing H 2 O 2 and generating highly oxidative hydroxyl radicals ( •OH ) in tumor sites. Their application isn't limited by tumor grown depth or hypoxic microenvironment. However, the reaction efficiency is still hampered due to the structure of catalytic agents and the requirement for low pH environment. Here, we design a porous CuO nanocluster (CuO NC) through self-assembly of oleylamine stabilized CuO NPs (OAm-CuO NPs), and functionalize it with folic acid (CuO NC-FA) for specific tumor cell targeting. It can catalyze H 2 O 2 with high efficiency in nearly neutral environment. Besides, the porous structure of CuO NC also helps the diffusion of H 2 O 2 to the interior of nanocluster to further improve Fenton-like reaction efficiency. The convenient synthesis of CuO NC-FA with good Fenton-like reaction efficiency at neutral environment demonstrates good chemodynamic therapy effect.
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http://dx.doi.org/10.1002/asia.202200296DOI Listing
June 2022

Research progress in the role and mechanism of LPCAT3 in metabolic related diseases and cancer.

J Cancer 2022 1;13(8):2430-2439. Epub 2022 May 1.

Institute of Digestive Diseases, Longhua Hospital, Shanghai University of Traditional Chinese Medicine - Shanghai 200032, China.

Lysophosphatidylcholine acyltransferases (LPCATs) are among the lysophopholipid acyltransferases (LPLATs) that specifically regulate the abundance of different phosphatidylcholine (PC) species in a variety of cell and tissue types, thereby playing an important role in lipid metabolism and homeostasis. Lysophosphatidylcholine acyltransferase 3 (LPCAT3, MBOAT5) is a member of the LPCAT family that primarily regulates the levels of arachidonic PC species. LPCAT3 is regulated by the liver X receptor, which plays an important role in lipoprotein production in the liver and small intestine. Increasing lines of research have demonstrated that LPCAT3 plays important roles in the occurrence and development of many diseases, such as atherosclerosis, intestinal tumors, and nonalcoholic steatohepatitis (NASH). The development of many diseases has been linked to the proinflammatory effects of LPCAT3. This review focuses on the current knowledge of LPCAT3, including its function and mechanism in different diseases. We aim to provide a comprehensive and in-depth understanding of LPCAT3 and to provide new ideas for the treatment of some diseases.
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http://dx.doi.org/10.7150/jca.71619DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9174858PMC
May 2022

Fumarate suppresses B-cell activation and function through direct inactivation of LYN.

Nat Chem Biol 2022 Jun 16. Epub 2022 Jun 16.

School of Life Sciences, Tsinghua University, Beijing, China.

Activated B cells increase central carbon metabolism to fulfill their bioenergetic demands, yet the mechanistic basis for this, as well as metabolic regulation in B cells, remains largely unknown. Here, we demonstrate that B-cell activation reprograms the tricarboxylic acid cycle and boosts the expression of fumarate hydratase (FH), leading to decreased cellular fumarate abundance. Fumarate accumulation by FH inhibition or dimethyl-fumarate treatment suppresses B-cell activation, proliferation and antibody production. Mechanistically, fumarate is a covalent inhibitor of tyrosine kinase LYN, a key component of the BCR signaling pathway. Fumarate can directly succinate LYN at C381 and abrogate LYN activity, resulting in a block to B-cell activation and function in vitro and in vivo. Therefore, our findings uncover a previously unappreciated metabolic regulation of B cells, and reveal LYN is a natural sensor of fumarate, connecting cellular metabolism to B-cell antigen receptor signaling.
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http://dx.doi.org/10.1038/s41589-022-01052-0DOI Listing
June 2022

Assessment of efficacy and prognostic factors by Gelfoam for DEB-TACE in unresectable large hepatocellular carcinoma with portal vein tumor thrombus: a multi-center retrospective study.

Expert Rev Gastroenterol Hepatol 2022 Jun 21:1-8. Epub 2022 Jun 21.

Department of Radiology, Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning Province, China.

Objective: To explore the clinical efficacy and prognostic factors of the use of Gelfoam for drug-eluting bead (DEB) transarterial chemoembolization (GMD-TACE) in patients with unresectable large hepatocellular carcinoma (HCC) and portal vein tumor thrombus (PVTT).

Methods: A retrospective analysis was conducted using the mRECIST standard to evaluate tumor response after GMD-TACE. Overall survival time, median survival time, time to progression (TTP) after the first intervention, and other treatment methods were recorded.

Results: The follow-up time was 2-110 months (mean 17.97 + 19.12 months), the median follow-up time was 12.5 months, and the first TTP after the first GMD-TACE was 4 months (95% CI 3.020-4.980). The median overall survival (OS) time was 14 months (95% CI 9.801-18.199). The 1-, 3-, and 5-year survival rates were 53.6%, 32.3%, and 8.9%, respectively. Multivariate analysis showed that the type of tumor thrombus was an independent factors affecting prognosis, and combination therapy was a protective factor affecting prognosis.

Conclusions: GMD-TACE can be used as the core treatment for unresectable large HCC combined with a PVTT. This can improve the quality of life and further improve the median OS, and is worthy of clinical promotion and application.
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http://dx.doi.org/10.1080/17474124.2022.2091545DOI Listing
June 2022
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