Publications by authors named "Ying Liang"

789 Publications

Effect of multiple freezing/thawing cycles on the physicochemical properties and structural characteristics of starch from wheat flours with different gluten strength.

Int J Biol Macromol 2021 Nov 22. Epub 2021 Nov 22.

College of Food Science and Engineering, Henan University of Technology, Zhengzhou 450001, China.

The physicochemical properties and structural characteristics of starches from three wheat flours with different gluten strength (S-YM20, S-ZM27, and S-ZM366) during freezing/thawing (F/T) cycles were studied. After F/T treatment, the damaged starch content of these three starches all increased, and the lowest increment of damaged starch content after 8 F/T cycles was S-ZM366; the most serious distribution of particle surface concave hole and fracture was S-YM20, followed by S-ZM27 and S-ZM366; additionally, the results of solubility, swelling power, thermal stability and pasting properties indicated S-ZM366 exhibited the strongest resistance to F/T cycles. The differences of freezing resistance among the three starches were possibly ascribed to the differences in compositions, crystallinity and microstructure among these three starches. This study provides theoretical contribution to the development of frozen dough industry from the perspective of wheat variety.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.11.105DOI Listing
November 2021

Antibacterial fluorescent nano-sized lanthanum-doped carbon quantum dot embedded polyvinyl alcohol for accelerated wound healing.

J Colloid Interface Sci 2021 Oct 7;608(Pt 1):973-983. Epub 2021 Oct 7.

National and Local Joint Engineering Research Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, 210023 Nanjing, China; Jiangsu Engineering Research Center of Interfacial Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, 163 Xianlin Avenue, Qixia District, Nanjing 210023, China. Electronic address:

Bacteria is one of the main culprits that cause human diseases and pose long-term challenges to people's health. Rare earth elements have unique antibacterial advantages, but little research is available. In this paper, we reported an antibacterial composite film based on lanthanum-doped carbon quantum dot nanoparticles ([email protected]) and polyvinyl alcohol (PVA) film for fluorescence of antibiotics and accelerating wound healing. PVA/[email protected] composite film presented excellent hydrophilicity, biocompatibility, fluorescence intensity, and antibacterial effects. The antibacterial activity of [email protected] was evaluated by employing antibacterial assay using Escherichia coli (E.coli)and Staphylococcus aureus (S.aureus) in vitro. [email protected] showed enhanced antibacterial activity compared with N-P-CQDs. Moreover, the PVA/[email protected] composite film with 0.5 mg/mL [email protected] showed better antibacterial capability and wound healing performance than PVA and PVA/N-P-CQDs films in bacterial adhesion experiment. PVA/[email protected] composite film could be used for wound dressing in vivo experiment and had no side effects on major organs in mice. The antibacterial composite film significantly promoted in vivo wound healing process because of its multifunctional properties. Therefore, it was an excellent candidate for wound dressing.
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http://dx.doi.org/10.1016/j.jcis.2021.10.018DOI Listing
October 2021

Corrigendum to 'High mobility group box 1 antibody represses autophagy and alleviates hippocampus damage in pilocarpine-induced mouse epilepsy model' [Acta Histochemica 122 (2020) 151485].

Acta Histochem 2021 Nov 11:151815. Epub 2021 Nov 11.

Department of Neurology, Qilu Hospital of Shandong University, Jinan, Shandong 250012, People's Republic of China. Electronic address:

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http://dx.doi.org/10.1016/j.acthis.2021.151815DOI Listing
November 2021

NR4A2 alleviates cardiomyocyte loss and myocardial injury in rats by transcriptionally suppressing CCR5 and inducing M2 polarization of macrophages.

Microvasc Res 2021 Nov 10;140:104279. Epub 2021 Nov 10.

Department of Cardiology, The People's Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, Guangxi Zhuang Autonomous Region, PR China. Electronic address:

Background: CC chemokine receptor 5 (CCR5) has been demonstrated to be correlated to activation of pro-inflammatory immune cells and tissue injury. This study focused on the role of CCR5 in myocardial injury in rats with diabetic cardiomyopathy (DCM) and the mechanism of action.

Methods: A rat model of DCM was induced by streptozotocin (STZ). CCR5 was knocked down in rats to determine its role in myocardial injury and immune cell infiltration. The upstream regulators of CCR5 were bioinformatically predicted and the binding between nuclear receptor subfamily 4 group A member 2 (NR4A2) and CCR5 was validated. The portion of M1 and M2 macrophages in tissues was determined by flow cytometry or double-labeling immunofluorescence. Rat bone marrow mononuclear cells (BMMCs) were treated with granulocyte/macrophage colony stimulating factor (GM-CSF/M-CSF) and co-cultured with H9C2 cells for in vitro experiments.

Results: STZ-treated rats had impaired cardiac function and increased levels of creatine kinase-MB, cardiac troponin I and lactate dehydrogenase. CCR5 inhibition significantly alleviated myocardial injury in rats and reduced the portion of M1 macrophages in rat cardiac tissues. NR4A2, which could suppress CCR5 transcription, was poorly expressed in rats with DCM. NR4A2 overexpression played a similar myocardium-protective role in rats. In vitro, overexpression of NR4A2 induced M2 polarization of macrophages, which protected the co-cultured H9C2 cells from high glucose-induced damage, but the protective role was blocked after CCR5 overexpression.

Conclusion: This study demonstrated that NR4A2 suppresses CCR5 expression and promotes M2 polarization of macrophages to alleviate cardiomyocyte loss and myocardial injury.
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http://dx.doi.org/10.1016/j.mvr.2021.104279DOI Listing
November 2021

Measurement of cell compressibility changes during epithelial-mesenchymal transition based on acoustofluidic microdevice.

Biomicrofluidics 2021 Dec 8;15(6):064101. Epub 2021 Nov 8.

Sino-French Institute of Nuclear Engineering and Technology, Sun Yat-sen University, Zhuhai 519082, China.

Epithelial-mesenchymal transition (EMT) confers migratory and invasiveness abilities on cancer cells, as well as leading to changes in biomechanical properties and cytoskeletal structure. Cell mechanical properties are considered to be promising label-free markers for diagnosis of cancer metastasis. In this work, cell compressibility, a novel and important parameter of cell mechanical properties, was measured directly and quickly using a specially designed acoustofluidic microdevice. The compressibilities of cells with different metastatic potentials were investigated. Based on a comparison of the measurement results, non-metastatic cells exhibited lower compressibility than metastatic cells. The correlation between cell compressibility and EMT status was further studied; the results showed that the acquisition of mesenchymal status was accompanied by an increase in cell compressibility. These findings imply strong correlations among cell compressibility, EMT status, and invasiveness. Therefore, cell compressibility represents a novel biomechanical marker for evaluating malignant transformation and metastasis of cancer.
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http://dx.doi.org/10.1063/5.0072126DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8577866PMC
December 2021

Did breeding alter the light environment, photosynthetic apparatus and photosynthetic capacity of wheat leaves?

J Exp Bot 2021 Nov 10. Epub 2021 Nov 10.

State Key Lab of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong Province, China.

Whether photosynthesis has improved with increasing yield in major crops remains controversial. Research in this area has often neglected to account for differences in light intensity experienced by cultivars released in different years. Light intensity is expected to be positively associated with photosynthetic capacity and resistance of the photosynthetic apparatus to high light but negatively associated with light utilization efficiency under low light. Here, we analyzed the light environment, photosynthetic activity, and protein components of leaves of 26 winter wheat cultivars released during the past 60 years in China. Over time, light levels on flag leaves significantly decreased due to architectural changes, but photosynthetic rates under high or low light and the resistance of the photosynthetic apparatus to high light remained steady, contrary to expectations. We propose that the difference between the actual and expected trends is due to breeding. Specifically, breeding has optimized photosynthetic performance under high light rather than low light. Moreover, breeding selectivity altered the stoichiometry of several proteins related to dynamic photosynthesis, canopy light distribution, and photoprotection. These results indicate that breeding has significantly altered the photosynthetic mechanism in wheat and its response to the light environment. These changes likely have helped increase wheat yields.
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http://dx.doi.org/10.1093/jxb/erab495DOI Listing
November 2021

Inflammatory alveolar macrophage-derived microvesicles damage lung epithelial cells and induce lung injury.

Immunol Lett 2021 Nov 2;241:23-34. Epub 2021 Nov 2.

Department of Cardiopulmonary Bypass, State key Laboratory of Cardiovascular Disease, National Center for Cardiovascular Diseases, Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. Electronic address:

Emerging evidence has demonstrated that several microvesicles (MVs) are secreted in bronchoalveolar lavage fluid (BALF) during the pathogenesis of acute lung injury/acute respiratory distress syndrome (ALI/ARDS). However, the impact of alveolar macrophage (AM)-derived MVs on epithelial cells and their in vivo effects on ALI/ARDS require further exploration. In this study, MVs were isolated from BALF of mice or mouse alveolar macrophage (MHS) cells by sequential centrifugation and then delivered to epithelial cells or mice. Enzyme-linked immunosorbent assay revealed that BALF-derived MVs (BALF-MVs) and MHS-derived MVs (AM-MVs) were rich in tumor necrosis factor-α (TNF-α) at the early stage of lung injury. In vitro, both inflammatory BALF-MVs and AM-MVs decreased the expression of α subunit of epithelial sodium channel (α-ENaC), γ-ENaC, and Na,K-ATPase α1 and β1 in lung epithelial cells. However, antibodies against TNF-α inhibited the effects of inflammatory AM-MVs in epithelial cells. In vivo, the inflammatory AM-MVs, delivered intratracheally to mice, impaired lung tissues and increased the injury score. They also resulted in decreased alveolar fluid clearance and increased lung wet weight/dry weight ratio. Furthermore, inflammatory AM-MVs downregulated the α-ENaC, γ-ENaC, and Na,K-ATPase α1 and β1 levels in lung tissues. According to our results, inflammatory AM-derived MVs may potentially contribute to lung injury and pulmonary edema, thereby indicating a potential novel therapeutic approach against ALI/ARDS based on AM-MVs.
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http://dx.doi.org/10.1016/j.imlet.2021.10.008DOI Listing
November 2021

Multifunctional Switchable Nanocoated Membranes for Efficient Integrated Purification of Oil/Water Emulsions.

ACS Appl Mater Interfaces 2021 Nov 4;13(45):54315-54323. Epub 2021 Nov 4.

Civil & Environmental Engineering Department, University of Nebraska-Lincoln, Omaha, Nebraska 68182-0178, United States.

Surfaces with unusual under-liquid dual superlyophobicity are attractive on account of their widespread applications, but their development remains difficult due to thermodynamic contradiction. Additionally, these surfaces may suffer from limited antifouling ability, which has restricted their practical applications. Herein, we report a successful in situ growth of a hybrid zeolitic imidazolate framework-8 and zinc oxide nanorod on a porous poly(vinylidene fluoride) membrane ([email protected]) and its application as a self-cleaning switchable barrier material in rapid filtration for emulsified oily wastewater. The novel [email protected] exhibits superior mechanical strength, reversible under-liquid dual superlyophobicity, photocatalytic self-cleaning property, and an effective alternate separation capacity toward both oil-in-water (O/W) and water-in-oil (W/O) emulsions with ultrahigh fluxes and efficiencies (>99%). By simply using a "bait-hook-eliminate" method to separate the O/W emulsions containing soluble organic pollutants, we demonstrate that the [email protected] can achieve stable separation fluxes over 600 L m h with high efficiencies and be completely/nondestructively regenerated by visible-light irradiation after each cycle. This study would demonstrate a new approach to prepare an under-liquid dual superlyophobic revivable membrane for various applications.
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http://dx.doi.org/10.1021/acsami.1c15024DOI Listing
November 2021

Epitopes prediction for microcystin-LR by molecular docking.

Ecotoxicol Environ Saf 2021 Dec 28;227:112925. Epub 2021 Oct 28.

School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China; Key Laboratory of Food Quality and Safety of Jiangsu Province, Nanjing 210014, China.

Microcystin-LR (MC-LR) is one of the most worldwide harmful cyanobacterial toxins. A lots of antibodies against MC-LR have been generated and characterized. However, the knowledge about the epitopes of MC-LR was still limited. The objective of this study was to analyze the epitopes of MC-LR and demonstrate the binding mode of MC-LR with its antibody. The variable genes of a mouse hybridoma cell line (Mab5H1-3B3) raised against MC-LR have been cloned and assembled in a single chain variable fragment (scFv), and then soluble expressed in E.coli BL21. Based on the scFv, the IC and IC for MC-LR were determined to be 7.45 nM and 0.30 nM by competitive ELISA. And the scFv also showed 115% and 112% cross-reactivities to MC-RR and MC-YR, and 59% to MC-LA. By molecular docking, the binding mode between MC-LR and its scFv was demonstrated. A hydrogen bond interaction was observed between the carbonyl group of Adda residue of MC-LR and its scFv, and the guanidyl group of Arg residue and phenyl group of Adda residue of MC-LR were also involved in the interaction. These predicted epitopes were supported by antibody cross-reactivity data. By comparing the antibody informatics of MC-LR scFv with its predicted paratopes, VH-CDR1 was crucial for MC-LR binding, and its specificity could be tuned by engineering in Vκ-CDR1 and Vκ-CDR3. These information would be useful for the hapten design for microcystins or improving the properties of MC-LR scFv in vitro.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112925DOI Listing
December 2021

COVID-19: risk factors for severe cases of the Delta variant.

Aging (Albany NY) 2021 10 28;13(20):23459-23470. Epub 2021 Oct 28.

Guangzhou Eighth People's Hospital, Guangzhou Medical University, Guangzhou, China.

Background: Since April 2021, the SARS-CoV-2 (B.1.167) Delta variant has been rampant worldwide. Recently, this variant has spread in Guangzhou, China. Our objective was to characterize the clinical features and risk factors of severe cases of the Delta variant in Guangzhou.

Methods: A total of 144 patients with the Delta variant were enrolled, and the data between the severe and non-severe groups were compared. Logistic regression methods and Cox multivariate regression analysis were used to investigate the risk factors of severe cases.

Results: The severity of the Delta variant was 11.1%. Each 1-year increase in age (OR, 1.089; 95% CI, 1.035-1.147; = 0.001) and each 1-μmol/L increase in total bilirubin (OR, 1.198; 95% CI, 1.021-1.406; = 0.039) were risk factors for severe cases. Moreover, the risk of progression to severe cases increased 13.444-fold and 3.922-fold when the age was greater than 58.5 years (HR, 13.444; 95% CI, 2.989-60.480; = 0.001) or the total bilirubin level was greater than 7.23 μmol/L (HR, 3.922; 95% CI, 1.260-12.207; = 0.018), respectively.

Conclusion: Older age and elevated total bilirubin were independent risk factors for severe cases of the Delta variant in Guangzhou, especially if the age was greater than 58.5 years or the total bilirubin level was greater than 7.23 μmol/L.
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http://dx.doi.org/10.18632/aging.203655DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8580340PMC
October 2021

Determination of ammonium in natural water using a quinoline-based -dialdehyde fluorescent reagent with visible excitation wavelength.

Anal Methods 2021 Nov 11;13(43):5231-5239. Epub 2021 Nov 11.

School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, Guangxi, 541004, China.

In this paper, a novel and stable fluorescent reagent, quinoline-2,3-dicarbaldehyde (QDA), is synthesized as a probe to detect ammonium in natural water. Ammonium reacts with QDA in the presence of SO and Ca to form a fluorescence product, which has maximum excitation and emission wavelengths at 429 nm and 518 nm. The concentration of reagents, the reaction temperature, the reaction time, and the pH in the final solution are investigated and optimized. The interferences of typical organic nitrogen and inorganic compounds are evaluated, and results prove that most volatile amines have little or negligible effect. Under the optimized conditions, this method provides a limit of detection of 0.065 μmol L, a calibration range of 0.216-9 μmol L, and reproducibility (with a relative standard deviation) of 1.9% for 1.5 μmol L ammonium. For water sample analysis, the proposed method provides comparable results to those of the conventional -phthalaldehyde method but has longer reagent stability (42 days).
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http://dx.doi.org/10.1039/d1ay01462eDOI Listing
November 2021

An Effective Method to Accurately Extract the Parameters of Single Diode Model of Solar Cells.

Nanomaterials (Basel) 2021 Oct 4;11(10). Epub 2021 Oct 4.

College of Information Science and Engineering, Huaqiao University, Xiamen 361021, China.

A non-iterative method is presented to accurately extract the five parameters of single diode model of solar cells in this paper. This method overcomes the problems of complexity and accuracy by simplifying the calculation process. Key parts of the equation are to be adjusted dynamically so that the desired five parameters can be obtained from the curve. Then, the and characteristic curves of solar cells are used to compare the effectiveness of this method with other methods. Furthermore, the root mean square error analysis shows that this method is more applicable than other methods. Finally, the and characteristics simulated by using the extracted parameters in this method are compared and discussed with the experimental data of solar cells under different conditions. In fact, this extraction process can be regarded as an effective and accurate method to estimate solar cells' single diode model parameters.
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http://dx.doi.org/10.3390/nano11102615DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539323PMC
October 2021

Functional Mechanism of Antimicrobial Peptide Bomidin and Its Safety for Macrobrachium rosenbergii.

Probiotics Antimicrob Proteins 2021 Oct 12. Epub 2021 Oct 12.

MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.

Macrobrachium rosenbergii is an economically important source of crustacean seafood worldwide. Vibrio parahaemolyticus is an important aquatic pathogen that causes epidemics of acute hepatopancreatic necrosis in shrimp populations, which results in significant economic losses to aquaculture farmers. To prevent the antibiotics abuse, which has become a serious threat to human health, novel anti-infective strategies are urgently required to control V. parahaemolyticus. Antimicrobial peptides, which exhibit favourable germicidal activity compared to traditional antibiotics, can be used as a key method to prevent and treat bacterial diseases. Herein, an antimicrobial peptide, bomidin, was expressed through genetic engineering technology. The minimum inhibitory concentration (MIC) of bomidin showed a significant inhibitory effect on V. parahaemolyticus that was equivalent to that of ampicillin. Subsequently, the mechanism of action of recombinant bomidin was explored using PNP and ONPG assays to investigate the effects on membrane permeability. These assays indicated that bomidin penetrated the germ membrane and induced the release of cytoplasmic contents and ultimately interacted with DNA to form a bomidin-DNA complex that inhibits bacterial survival. Transmission electron microscopy and scanning electron microscopy revealed that bomidin could cause damage and dysfunction to the cell wall and membrane. Bomidin was nontoxic to mouse red blood cells within a concentration range that was much larger than the MIC. Toxicity assays revealed that 0.02 mg/mL bomidin was safe for use with juvenile freshwater prawns of M. rosenbergii and significantly inhibited the growth of V. parahaemolyticus in cultured water. These results demonstrated that synthetic peptide bomidin had great antibacterial effect against V. parahaemolyticus and therefore a therapeutic potential in aquaculture.
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http://dx.doi.org/10.1007/s12602-021-09857-6DOI Listing
October 2021

A novel multi-layer prediction approach for sweetness evaluation based on systematic machine learning modeling.

Food Chem 2022 Mar 28;372:131249. Epub 2021 Sep 28.

Xiangya School of Pharmaceutical Sciences, Central South University, Changsha 410013, PR China. Electronic address:

Nowadays, computational approaches have drawn more and more attention when exploring the relationship between sweetness and chemical structure instead of traditional experimental tests. In this work, we proposed a novel multi-layer sweetness evaluation system based on machine learning methods. It can be used to evaluate sweet properties of compounds with different chemical spaces and categories, including natural, artificial, carbohydrate, non-carbohydrate, nutritive and non-nutritive ones, suitable for different application scenarios. Furthermore, it provided quantitative predictions of sweetness. In addition, sweetness-related chemical basis and structure transforming rules were obtained by using molecular cloud and matched molecular pair analysis (MMPA) methods. This work systematically improved the data quality, explored the best machine learning algorithm and molecular characterizing strategy, and finally obtained robust models to establish a multi-layer prediction system (available at: https://github.com/ifyoungnet/ChemSweet). We hope that this study could facilitate food scientists with efficient screening and precise development of high-quality sweeteners.
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http://dx.doi.org/10.1016/j.foodchem.2021.131249DOI Listing
March 2022

Functionalized triazine-based covalent organic frameworks containing quinoline via aza-Diels-Alder reaction for enhanced lithium-sulfur batteries performance.

J Colloid Interface Sci 2021 Sep 27;608(Pt 1):652-661. Epub 2021 Sep 27.

State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, PR China. Electronic address:

The development of functional covalent organic frameworks (COFs) with specific properties is an emerging research field. In the current work, COF-SQ-Ph was synthesized through the aza-Diels-Alder reaction between phenylacetylene and the matrix COF-SQ (triazine-based COF) generated from the organic monomers 2, 4, 6-tris(4-aminophenyl)-1, 3, 5-triazine and 2, 5-dimethoxyterephthalaldehyde in flask. The functionalized COF-SQ-Ph with an extended π-conjugated structure and enhanced structural stability was used as the sulfur loading recipient to prepare sulfur cathodes for lithium-sulfur batteries. Sulfur-impregnated COF-SQ-Ph marked as COF-SQ-Ph-S displayed better cycling stability with a specific capacity of 618 mA h g after 150 cycles due to the lithiophilic interaction between lithium polysulfides and nitrogen atoms from quinoline and triazine moieties in COF-SQ-Ph-S. The functionalization of triazine-based COFs through a cycloaddition reaction in flask could promote the large-scale preparation of tailored COFs and the post-synthesis modification of COF-SQ.
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http://dx.doi.org/10.1016/j.jcis.2021.09.150DOI Listing
September 2021

Sensitive electrochemical aptasensor for determination of sulfaquinoxaline based on AuPd [email protected]/CoSe and RecJf exonuclease-assisted signal amplification.

Anal Chim Acta 2021 Oct 13;1182:338948. Epub 2021 Aug 13.

School of Food Science and Technology, Henan University of Technology, Zhengzhou, Henan, 450001, PR China.

The authors designed a sensitive label-free electrochemical aptasensor for the detection of sulfaquinoxaline (SQX), including the AuPd [email protected]/CoSe nanocomposites and RecJf exonuclease-assisted target recycle signal amplification strategy. AuPd [email protected]/CoSe nanocomposite with excellent conductivity and numerous active sites was successfully synthesized to provide a favorable sensing platform and load more double-strand DNA (dsDNA) on the electrode surface. The negatively charged phosphate group of the oligonucleotide and [Fe (CN)] repel each other electrostatically, resulting in very low electrical signals. In the presence of SQX, its corresponding aptamer will be released from the double-stranded structure and then digested by RecJf exonuclease, which resulted in the SQX being released to initiate the next recycling to help amplify the DPV signal. Under the optimal conditions, the peak current has a linear relationship with the logarithmic of SQX concentration in the range of 1 pg/mL∼100 ng/mL and the obtained detection limit was 0.547 pg/mL. Furthermore, the contrasted aptasensor possess reliable specificity, reproducibility and stability toward SQX, and has been applied to detect SQX in pork samples with a satisfied recovery varied from 94.40% to 95.98%.
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http://dx.doi.org/10.1016/j.aca.2021.338948DOI Listing
October 2021

Intermittent exposure to airborne particulate matter induces subcellular dysfunction and aortic cell damage in BALB/c mice through multi-endpoint assessment at environmentally relevant concentrations.

J Hazard Mater 2021 Sep 9;424(Pt A):127169. Epub 2021 Sep 9.

Key Laboratory of Environmental Medicine Engineering of Ministry of Education, Southeast University, Nanjing, Jiangsu 210009, China. Electronic address:

Airborne particulate matter (PM) has been linked to cardiovascular diseases, but the underlying mechanisms remain unclear, especially at realistic exposure levels. In this study, both male and female BALB/c mice were employed to assess vascular homeostasis following a standard urban particulate matter, PM SRM1648a, via oropharyngeal aspiration at three environmentally relevant concentrations. The tested indicators included histopathological observation and lipid deposition, as well as redox biology and inflammatory responses. Furthermore, endothelial monolayer, vascular cell apoptosis and subcellular function were assessed to decipher whether episodic PM SRM1648a exposure leads to vascular damage after multiple periods of treatment, including subacute (4 weeks) and subchronic (8 weeks) durations. As a result, PM aspiration caused thickening of airways, leukocytes infiltration and adhesion to alveoli, with the spot of particles engulfed by pulmonary macrophages. Meanwhile, it induced local and systemic oxidative stress and inflammation, but limited pathological changes were captured throughout aortic tissues after either subacute or subchronic treatment. Furthermore, even in the absence of aortic impairment, vascular cell equilibrium has been disturbed by the characteristics of endothelial monolayer disintegration and cell apoptosis. Mechanistically, PM SRM1648a activated molecular markers of ER stress (BIP) and mitochondrial dynamics (DRP1) at both transcriptional and translational levels, which were strongly correlated to ox-inflammation and could serve as early checkpoints of hazardous events. In summary, our data basically indicate that episodic exposure of BALB/c mice to PM SRM1648a exerts limited effects on vascular histopathological alterations, but induces vascular cell apoptosis and subcellular dysfunction, to which local and systemic redox biology and inflammation are probably correlated.
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http://dx.doi.org/10.1016/j.jhazmat.2021.127169DOI Listing
September 2021

The Relationship Between Mental Health Problems and Systemic Family Dynamics Among High School and University Students in Shaanxi Province, China.

Int J Public Health 2021 6;66:1603988. Epub 2021 Sep 6.

Department of Health Statistics and Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, Air Force Medical University, Xi'an, China.

The present study aimed to correlate relationships between systemic family dynamics and mental health and to explore family factors that influence adolescent mental health in Shaanxi Province, China. A cross-sectional survey was conducted to sample adolescents aged 12-23 using a questionnaire including Self-rating Scale of Systemic Family Dynamics, Symptom Checklist-90 Revised, and general demographic. More educated parents in white-collar employment and higher family income were associated with better mental health and better family dynamic scores. The total score of family dynamics was positively correlated with mental health scores. The generalized linear mixed model found that poorer mental health was associated with increased age, being in senior high school, having a father in a blue-collar profession, and SSFD square. The structural equation modelling suggested that this is largely a mediated effect those characteristics impacting family dynamics, which in turn affect mental health. Family dynamics may be an important contributor to adolescent mental health. Education and interventions aimed at improving family dynamics may be useful for reducing the prevalence of mental health problems amongst adolescents.
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http://dx.doi.org/10.3389/ijph.2021.1603988DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8450291PMC
September 2021

A single-center analysis of outcomes, risk factors, and new valves in Asian patients treated with early transcatheter aortic valve implantation.

Cardiovasc Diagn Ther 2021 Aug;11(4):967-979

Department of Extracorporeal Circulation, Fuwai Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.

Background: Transcatheter aortic valve implantation (TAVI) continues to expand as an optimal treatment in Western countries; however, Asian countries have been slower to adopt this procedure. This research aimed to explore the outcomes and status of early TAVI performed at a single center in Asia, and provide comparative outcomes of the newly designed Chinese valves.

Methods: We enrolled 175 consecutive patients who successfully underwent TAVI from September 2012 to January 2018 at Fuwai Hospital (Beijing, China). After a preliminary assessment of age, we included 109 older patients (≥69 years) who underwent surgical aortic valve replacement (SAVR) during the same period. The primary endpoint was all-cause mortality. The inverse probability of treatment weighting (IPTW) was used to reduce potential bias. Cox regression was used to identify the risk factors of a poor prognosis.

Results: The TAVI cohort had higher rates of all-cause mortality [11.4% 2.4%, hazard ratio (HR): 4.79, 95% confidence interval (CI): 1.47 to 15.57, IPTW-adjusted P=0.009] and permanent pacemaker implantation (PPI; 14.6% 1.6%, HR: 9.98, 95% CI: 2.71 to 36.67, IPTW-adjusted P<0.001) at 3 years than the SAVR cohort. In the multivariable Cox regression analysis based on the entire sample, liver disease was associated with all-cause mortality (HR: 5.080, 95% CI: 1.067 to 24.174, P=0.041). A smoking history was associated with an increased risk of postoperative heart failure (HF) (HR: 4.902, 95% CI: 1.265 to 18.999, P=0.022). Additionally, age (HR: 1.141, 95% CI: 1.010 to 1.288, P=0.034) and diabetes (HR: 7.301, 95% CI: 2.414 to 22.079, P<0.001) were identified as predictors of postoperative stroke. In the new valve subgroups, the 1-year composite endpoints were 38.2% (Venus A), 35.3% (TaurusOne), 34% (J-Valve), and 28% (VitaFlow) (P=0.857).

Conclusions: Not all TAVI procedures had satisfactory outcomes compared with SAVR when initiated. At first, our center faced some challenges in delivering TAVI, and this is probably one of the reasons why the use of TAVI has developed slowly in Asia. Further investigations are needed to explore the underlying factors precluding the rapid expansion of TAVI in Asia.
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http://dx.doi.org/10.21037/cdt-20-928DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8410484PMC
August 2021

Dengzhanxixin Injection Ameliorates Cognitive Impairment Through a Neuroprotective Mechanism Based on Mitochondrial Preservation in Patients With Acute Ischemic Stroke.

Front Pharmacol 2021 30;12:712436. Epub 2021 Aug 30.

State Key Laboratory of Cognitive Neuroscience and Learning & IDG/McGovern Institute for Brain Research, Beijing Normal University, Beijing, China.

Acute ischemic stroke (AIS) is a global health burden and cognitive impairment is one of its most serious complication. Adequate interventions for AIS may have the potential to improve cognitive outcomes. In the present study, we selected (Vaniot) Hand.-Mazz. injection (Dengzhanxixin injection, DZXI), a widely used Chinese herbal injection, in contrast to edaravone as the positive control drug to test its potential to ameliorates neurological and cognitive impairments caused by AIS. We performed a 2-week randomized trial with these two drugs in AIS patients presenting mild to moderate cognitive impairments. Neuropsychological tests and MRI examinations showed that DZXI attenuated the neurological and cognitive impairments of patients and protected the grey matter in specific regions from ischemic damage. Notably, DZXI exerted better effects than edaravone in some neuropsychological tests, probably due to the protective effect of DZXI on grey matter. To explore the therapeutic mechanisms, we carried out an experiment with a middle cerebral artery occlusion rat model. We found that DZXI decreased the infarct volume and increased the survival of neuronal cells in the ischemic penumbra; furthermore, DZXI modulated the mitochondrial respiratory chain process and preserved the mitochondrial structure in the brain tissue. Overall, our data suggested that the administration of DZXI is effective at ameliorating neurological and cognitive impairments in AIS, and the underlying mechanisms are related to the protective effects of DZXI on cerebral neurons and neuronal mitochondria.
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http://dx.doi.org/10.3389/fphar.2021.712436DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435665PMC
August 2021

Jianpi Qushi Heluo Formula alleviates renal damages in Passive Hemann nephritis in rats by upregulating Parkin-mediated mitochondrial autophagy.

Sci Rep 2021 09 15;11(1):18338. Epub 2021 Sep 15.

China Department of Nephrology, Xiyuan Hospital of China Academy of Chinese Medical Sciences, Beijing, 100091, China.

Jianpi Qushi Heluo Formula (JQHF) is an empirical traditional Chinese medicine prescription for treating Membranous Nephropathy (MN) clinically in China. The therapeutic effect of JQHF has been reported in our previous studies. However, the exact mechanism is still unknown. In this study, by establishing an experimental rat model of MN induced by Sheep anti-rat Fx1A serum, we evaluated the effects of JQHF and Tetrandrine (TET), and Benazepril was used as a positive control. As an autophagy agonist, TET is one of the most active components in JQHF. After 4 weeks, significant kidney damage was observed in the rats in the Model group; comparatively, JQHF markedly decreased 24 h urinary protein, Total Cholesterol (TC), and increased serum total Albumin (ALB). Histology showed that JQHF caused significant improvements in glomerular hyperplasia, renal tubular damage, IgG immune complex deposition, and the ultrastructure of mitochondria in MN rats. Flow cytometry analysis showed that treatment with JQHF reduced the level of reactive oxygen species and apoptosis rate, and upregulated mitochondrial membrane potential. Western blot analysis demonstrated that JQHF could protect against mitochondrial dysfunction and apoptosis by upregulating the expression of PINK1, Mitochondrial Parkin, and LC3-II/I, downregulating the expression of Cytoplasmic Parkin, P62, Cytochrome c, and Caspase-3 in the kidneys of MN rats. From images of co-immunofluorescence, it is observed significantly increase in the co-localization of PINK1 and Parkin, as well as LC3 and mitochondria. Similarly, TET treatment significantly upregulated the mitochondrial autophagy and reduced apoptosis in rats after 4 weeks compared with the model group. Comparatively, the ability of JQHF to alleviate renal damage was significantly higher than those of Benazepril and TET. It was demonstrated that JQHF could delay pathology damage to the kidney and hold back from the progression of MN by inhibiting apoptosis and upregulating the mitochondrial autophagy by PINK1/Parkin pathways.
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http://dx.doi.org/10.1038/s41598-021-97137-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8443625PMC
September 2021

Synergistic adsorption-photocatalytic degradation of different antibiotics in seawater by a porous g-CN/calcined-LDH and its application in synthetic mariculture wastewater.

J Hazard Mater 2021 08 23;416:126183. Epub 2021 May 23.

School of Environment, South China Normal University, University Town, Guangzhou 510006, China; Guangdong Technology Research Center for Ecological Management and Remediation of Urban Water System, Guangzhou 510006, China.

In this work, a modified g-CN/MgZnAl-calcined layered double hydroxide composite (M-CN/cLDH) was successfully fabricated via a template method. The composite material is a hierarchical porous flower-like nanostructure self-assembled from stacked hybrid flakes. The 3D M-CN/cLDH architectures exhibit a synergistic effect of adsorption and photocatalysis for eliminating typical tetracycline antibiotics in seawater, i.e., oxytetracycline (OTC), tetracycline (TC), chlortetracycline (CTC), and doxycycline (DXC). The synergistic removal rate of OTC in seawater of M-CN/cLDH is 2.73 times higher than that of g-CN after 120 min of visible-light illumination, and M-CN/cLDH also performs better adsorption-photocatalytic degradation on OTC in the continuous flow reaction process. The superior adsorption capability of the M-CN/cLDH is attributed to the open porous structures of cLDH, and its excellent photocatalytic degradation activity is ascribed to the closely bonded heterojunctions between g-CN (CN) and cLDH double layers. The mass spectra reveals the degradation pathways of OTC, and its byproducts are less toxic after degradation for 120 min. The exploration of the M-CN/cLDH in synthetic mariculture wastewater suggested a huge potential for its practical application. With the assistance of magnesium ammonium phosphate (MAP) precipitation pretreatment, the material can effectively retain the high OTC removal rate in the synthetic mariculture wastewater circumstance.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126183DOI Listing
August 2021

Clinical and Radiological Features of COPD Patients Living at ≥3000 m Above Sea Level in the Tibet Plateau.

Int J Chron Obstruct Pulmon Dis 2021 26;16:2445-2454. Epub 2021 Aug 26.

Department of Respiratory and Critical Care Medicine, Peking University Third Hospital, Beijing, 100191, People's Republic of China.

Background: COPD at high altitude may have different risk factors and unique clinical and radiological phenotypes. We aimed to investigate the demographic data, clinical and radiological features of COPD patients permanently residing at the Tibet Plateau (≥3000 meters above sea level).

Methods: We conducted an observational cross-sectional study which consecutively enrolled COPD patients visiting the outpatient of Respiratory Medicine at Tibet Autonomous Region People's Hospital from January 2018 to March 2021. All patients were Tibetan permanent residents aging ≥40 years and met the diagnosis of COPD according to Global Initiative for Chronic Obstructive Lung Disease (GOLD) guidelines. Data including demographic characteristics, altitude of residence, risk factors, respiratory symptoms, comorbidities and medications, as well as computed tomography (CT) measurements were collected.

Results: Eighty-four patients with definite COPD were enrolled for analysis. Their mean age was 64.7 (±9.1) years. All patients lived at ≥3000 m above sea level and 34.5% of them lived at ≥4000 m. About 8.3% of the patients were current smokers and 44.0% were ex-smokers. Up to 88.1% of the patients reported long-term exposure to indoor biomass fuels. Most of the patients were classified as having mild-to-moderate (GOLD I: 27.4%; GOLD II: 51.2%) COPD, while 89.3% had a CAT score ≥10. Only 36.9% of the patients received regular long-term medications for COPD in the past year, in whom ICS/LABA and oral theophylline were the most common used pharmacological therapy. On CT scanning, the majority of our patients (70.7%) showed no or minimal emphysema, while signs of previous tuberculosis were found in 45.1% of the patients.

Conclusion: COPD patients living at the Tibet Plateau had a heavy respiratory symptom burden, but most of them did not receive adequate pharmacological treatment. Indoor biomass fuel exposure and previous tuberculosis were prevalent, while the emphysema phenotype was less common in this population.
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http://dx.doi.org/10.2147/COPD.S325097DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8408343PMC
September 2021

A competitive-type electrochemical immunosensor based on [email protected] and [email protected] core-shell for nitrofuran metabolites residues detection.

Bioelectrochemistry 2021 Dec 21;142:107934. Epub 2021 Aug 21.

School of Food Science and Technology, Henan University of Technology, Zhengzhou, Henan 450001, PR China.

A novel competitive-type electrochemical immunosensor based on square wave voltammetry (SWV) response was developed for the quantitative detection of 1-Aminohydantoin (AHD). To improve the conductivity of this immunosensor nanocomposites with good electrical conductivity were prepared as a signal amplification platform for the immunosensor by growing Au nanoparticles on the surface of Ce-based metal-organic framework (Ce-MOF). In addition, methylene blue (MB)-loaded [email protected] and coating antigen (OVA-AHD) connected as a signal label. When the target was introduced, it competed with the coating antigen for the Ab, which led to a reduction in the number of signal probes bound to the Ab. The concentration of AHD can be determined by SWV detection of the MB signal loaded on the signal labels. Under optimal conditions, the wide linear range of 0.001-1000 μg /L and a low detection limit of 1.35 × 10 μg/L were achieved. Ultimately, the developed method displayed excellent specificity in practical applications, providing a promising probability to detect nitrofuran metabolites residues to guarantee food safety.
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http://dx.doi.org/10.1016/j.bioelechem.2021.107934DOI Listing
December 2021

Whole-body hyperthermia combined with chemotherapy and intensity-modulated radiotherapy for treatment of advanced nasopharyngeal carcinoma: a retrospective study with propensity score matching.

Int J Hyperthermia 2021 ;38(1):1304-1312

Department of Radiotherapy, Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou, PR China.

Background: Several studies have reported the combination of intracavity or cervical lymph node hyperthermia with chemoradiotherapy (CRT) to improve clinical outcomes in nasopharyngeal carcinoma (NPC), but the combination with whole-body hyperthermia (WBH) for treating NPC is unexplored. We aimed to assess the efficacy of the combination of radiotherapy, chemotherapy and WBH in patients with locoregionally advanced NPC.

Methods: Between July 2008 and November 2012, 239 newly diagnosed NPC patients were enrolled in a pre-propensity score-matched cohort, including 193 patients who received CRT (CRT group) and 46 who underwent CRT with WBH (HCRT group). The feasibility and clinical outcomes of both groups were evaluated and toxicities assessed. Survival rates were assessed using the Kaplan-Meier method, log-rank test and Cox regression.

Results: Following propensity score matching, 46 patients from each group were included. The 5-year overall survival (OS) rates were 65.2% in the CRT group and 80.3% in the HCRT group (=.027). In contrast, the other survival outcomes at 5 years were similar between the groups: locoregional recurrence-free survival (LRRFS), 74.7% 87.6% (=.152); distant metastasis-free survival (DMFS), 67.4% 77.9% (=.125); and progression-free survival (PFS), 53.1% 69.2% (=.115). In the multivariate analyses, the only two independent predictors of OS were clinical stage and HCRT.

Conclusions: These results suggest that WBH, when combined with CRT, can improve the OS of patients with advanced NPC.
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http://dx.doi.org/10.1080/02656736.2021.1971778DOI Listing
September 2021

Knockdown of MIR9‑3HG inhibits proliferation and promotes apoptosis of cervical cancer cells by miR‑498 via EP300.

Mol Med Rep 2021 Nov 1;24(5). Epub 2021 Sep 1.

Department of Gynecology and Obstetrics, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310006, P.R. China.

Cervical cancer is a serious gynecological cancer and one of the primary causes of mortality in female patients with cancer. Despite advances in cancer research, the molecular mechanism underlying cancer remains poorly understood. High levels of MIR9‑3 host gene (HG) are associated with the occurrence and development of cervical cancer. However, the specific role of MIR9‑3HG during the development of cervical cancer is unclear. In the present study, the expression of MIR9‑3HG was silenced in C33A and SiHa cervical cancer cell lines. Proliferation and apoptosis were measured in these cells using 5‑ethynyl‑2'‑deoxyuridine assay and flow cytometry, respectively. In addition, targeting microRNAs (miRs) of MIR9‑3HG and mRNAs of miR‑498 were predicted using public databases. The predicted interactions between these molecules were validated using RNA immunoprecipitation, RNA pull‑down and luciferase reporter assays. Lastly, C33A cells transfected with short hairpin MIR‑3HG alone or in combination with miR‑498 inhibitor or PC‑EP300 were subcutaneously injected into mice. The levels of miR‑498, EP300 and Ki67 in tumor tissue were measured via reverse transcription‑quantitative PCR or western blotting. MIR9‑3HG knockdown inhibited the proliferation of cervical cancer cells, whilst promoting apoptosis. MIR9‑3HG sponged miR‑498 and inhibited its expression. Additionally, miR‑498 interacted with EP300 and inhibited its expression. Transfection with miR‑498 inhibitor significantly decreased apoptosis levels; this effect was abolished following EP300 silencing . , both miR‑498 inhibition and EP300 overexpression reversed the inhibition of tumor growth mediated by MIR‑3HG knockdown. MIR9‑3HG promoted the proliferation cervical cancer cells via EP300 and miR‑498. These and findings demonstrate the regulatory role of the MIR9‑3HG/miR‑498/EP300 axis in cervical cancer cell growth. Thus, the present study identified novel molecular targets for the diagnosis and treatment of cervical cancer and provided new insight into the pathogenesis of cervical cancer.
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http://dx.doi.org/10.3892/mmr.2021.12388DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8430307PMC
November 2021

A review on graphitic carbon nitride (g-CN) based hybrid membranes for water and wastewater treatment.

Sci Total Environ 2021 Oct 16;792:148462. Epub 2021 Jun 16.

State Key Joint Laboratory of Environmental Simulation and Pollution Control, China-Canada Center for Energy, Environment and Ecology Research, UR-BNU, School of Environment, Beijing Normal University, Beijing 100875, China.

Graphitic carbon nitride (g-CN) has gained enormous attention for water and wastewater treatment. Compared with g-CN nanopowders, g-CN based hybrid membranes have demonstrated great potential for its superior practicability. This review outlines the preparation and characterization of g-CN based hybrid membranes and presents their representative applications in water and wastewater treatment (e.g., removal of organic dyes, phenolic compounds, pharmaceuticals, salt ions, heavy metals, and oils). Meanwhile, g-CN based films for the removal of contaminants through photocatalytic degradation is also summarized. In addition, the corresponding mechanisms and relevant findings are discussed. Finally, the challenges and research needs in the future and application of g-CN based hybrid membranes are highlighted.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148462DOI Listing
October 2021

The Associations Between White Matter Disruptions and Cognitive Decline at the Early Stage of Subcortical Vascular Cognitive Impairment: A Case-Control Study.

Front Aging Neurosci 2021 2;13:681208. Epub 2021 Aug 2.

Department of Neurology, China-Japan Friendship Hospital, Beijing, China.

Objective: Emerging evidence suggests that white matter (WM) disruption is associated with the incidence of subcortical vascular cognitive impairment (SVCI). However, our knowledge regarding this relationship in the early stage of SVCI is limited. We aimed to investigate the associations between WM disruptions and cognitive declines at the early stage of SVCI.

Method: We performed a case-control study, involving 22 cases and 19 controls. The cases were patients at the early stage of SVCI, which was defined as subcortical ischemic vascular disease with normal global cognitive measures (pre-SVCI). The controls were healthy people matched by age, sex, and education years. We assessed the differences in a battery of neuropsychological tests between the two groups, investigated the diffusion changes in 40 WM tracts among the participants an atlas-based segmentation strategy, and compared the differences between the cases and controls by multiple linear regression analysis. We then evaluated the relationships between diffusion indices and cognitive assessment scores by Pearson's correlation.

Results: The pre-SVCI group exhibited significant differences in the Montreal cognitive assessment (MoCA), Rey-Osterrieth Complex Figure (R-O)-copy, and Trail Making Test (TMT)-B test compared with the controls. Compared with the controls, some long associative and projective bundles, such as the right anterior corona radiata (ACR), the right inferior fronto-occipital fasciculus (IFOF), and the left external capsule (EC), were extensively damaged in cases after Bonferroni correction ( < 0.05/40). Damages to specific fibers, such as the right ACR, IFOF, and posterior thalamic radiation (PTR), exhibited significant correlations with declines in MoCA, R-O delay, and the Mini-Mental State Examination (MMSE), respectively, after Bonferroni correction ( < 0.05/14).

Conclusion: Long WM tracts, especially those in the right hemisphere, were extensively damaged in the pre-SVCI patients and correlated with declines in executive functions and spatial processing. Patients of pre-SVCI are likely at an ultra-early stage of SVCI, and there is a very high risk of this condition becoming SVCI.
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http://dx.doi.org/10.3389/fnagi.2021.681208DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8364958PMC
August 2021

ADGRG1 Is a Predictor of Chemoresistance and Poor Survival in Cervical Squamous Carcinoma.

Front Oncol 2021 22;11:671895. Epub 2021 Jul 22.

Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan, China.

Background: Cisplatin is the first-line chemotherapy for cervical cancer. Cisplatin resistance has always been one of the most significant barriers to acquiring better outcomes. However, the complex molecular mechanisms accounting for the phenomenon are not completely clear.

Methods: Construction of the cisplatin-resistant cell model of cervical cancer, then performing RNA sequencing and bioinformatic analysis of the differential expression genes. Then Adhesion G protein-coupled receptor G1 (ADGRG1) was screened out as our target gene. Gene Expression Profiling Interactive Analysis (GEPIA) was searched to show the expression level of ADGRG1 in cervical cancer and normal tissue. Kaplan-Meier Plotter (Kmplot) was used to explore the relationship of its expression with survival data. Tissue specimens were used to verify the relationship between the clinicopathological characteristics and ADGRG1 expression. Then we explored the roles of ADGRG1 in tumorigenesis through and assays.

Results: We found the ADGRG1 was significantly overexpressed in cervical cancer tissues compared to corresponding normal tissues. Higher ADGRG1 expression was correlated with poor progress-free survival. Knockdown of ADGRG1 markedly suppressed cell proliferation, migration, and invasion and increased cell sensitivity to cisplatin . Similarly, the role of ADGRG1 knockdown on tumorigenicity and sensitivity to cisplatin treatment was verified . The underlying mechanism was explored by western blotting that ADGRG1 knockdown inhibited tumorigenesis by PI3K/Akt/mTOR signaling pathway.

Conclusion: ADGRG1 acts as an oncogene to maintain tumorigenicity, migration, and invasion, and its depressed expression prompts sensitivity to cisplatin. Thus, ADGRG1 may represent a potential prognostic marker and possible therapeutic target for cervical cancer.
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http://dx.doi.org/10.3389/fonc.2021.671895DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8340018PMC
July 2021

Genetically Predicted Fibroblast Growth Factor 23 and Major Cardiovascular Diseases, Their Risk Factors, Kidney Function, and Longevity: A Two-Sample Mendelian Randomization Study.

Front Genet 2021 23;12:699455. Epub 2021 Jul 23.

LKS Faculty of Medicine, School of Public Health, The University of Hong Kong, Hong Kong, China.

Introduction: Fibroblast growth factor 23 (FGF23), a potential biomarker for kidney function, is related to cardiovascular disease (CVD) and diabetes, although it is unclear whether the relation is causal. This study evaluated the associations of genetically predicted FGF23 with major CVDs, their risk factors, kidney function, and longevity using Mendelian randomization (MR).

Methods: This is a two-sample MR study using summary statistics from large genome-wide association studies. Primary outcomes included coronary artery disease (CAD), myocardial infarction, heart failure, and atrial fibrillation. Secondary outcomes included cardiovascular risk factors, kidney function, and longevity. We used four single-nucleotide polymorphisms (SNPs) predicting FGF23, excluding rs2769071 in the gene, which likely violates the MR exclusion-restriction assumption. We used inverse-variance weighted (IVW) as the primary statistical method to assess associations of FGF23 with the outcomes. Sensitivity analyses included weighted median (WM) and MR-Egger. We repeated the analyses including all five SNPs. Last, we validated the positive findings from the main analyses in a smaller study, i.e., FinnGen.

Results: Using IVW, genetically predicted higher FGF23 was inversely associated with risk of CAD [odds ratio (OR): 0.69 per logtransformed FGF23 (pg/ml) increase, 95% confidence interval (CI): 0.52-0.91] and type 2 diabetes mellitus (T2DM) (OR: 0.70, 95% CI: 0.52-0.96), but not with the other outcomes. The WM and MR-Egger estimates were directionally consistent.

Conclusion: This study suggests that genetically predicted higher FGF23 may be protective against CAD and T2DM. Future studies should explore the underlying mechanisms related to the potential protective effect of FGF23. FGF23 was unlikely a cause of poorer renal function.
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http://dx.doi.org/10.3389/fgene.2021.699455DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8343174PMC
July 2021
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