Publications by authors named "Ying Gong"

92 Publications

An improved multi-variable grey model for forecasting China's finished products from comprehensive waste utilization.

Environ Sci Pollut Res Int 2021 Apr 7. Epub 2021 Apr 7.

College of Management Science and Engineering, Chongqing Technology and Business University, Chongqing, 400067, People's Republic of China.

A reasonable prediction of the finished products from waste recycling and reprocessing is of great significance to the sustainable use of limited resources, the reduced pollution caused by waste, and the reflected comprehensive waste utilization (CWU) creativity. To this end, an improved multi-variable grey model was employed to forecast the finished products in China's CWU. Firstly, the degree of grey incidence was applied to select explanatory variables and eliminate multicollinearity between them. Then, compared with the traditional GM(1,N) model, the linear correction term and grey action quantity were added in the proposed improved model, and the response function and parameter estimation method of the improved model were deduced and proved. Thirdly, the finished products from CWU was simulated and predicted by the proposed model. The mean relative simulation percentage error of the improved model was only 0.0001%, in comparison with the ones obtained from the traditional GM(1,N) and the classical GM(1,1), which were 12.1232 and 8.8402%, respectively. Lastly, the results show that the finished products from CWU are mainly affected by the comprehensive utilization of general industrial solid waste and the number of industrial enterprises in CWU, and the future trends from 2020 to 2025 are unstable.
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http://dx.doi.org/10.1007/s11356-021-13737-5DOI Listing
April 2021

Do Drug Accessibility and OOP Burden Affect Health-Related Quality of Life of Patients With Chronic Diseases? - EQ-5D-5L Evaluation Evidence From Five Districts in China.

Front Public Health 2021 12;9:656104. Epub 2021 Mar 12.

Faculty of Science, Lund University, Lund, Sweden.

The dependence of patients with chronic diseases on drugs may affect their health-related quality of life (HRQoL). This study aims to assess the relationship between the direct economic burden caused by out-of-pocket (OOP) payments, drug accessibility, sociodemographic characteristics, and health-related quality of life. 1,055 patients with chronic diseases from Gansu, Hebei, Sichuan, Zhejiang, and Tianjin were investigated. Data collection included basic conditions and economic and health insurance conditions of patients with chronic diseases. The CLAD and Tobit regression models were used to analyze and compare the health-related quality of life and influencing factors of patients with chronic diseases in five districts. Differentiated analysis was conducted through sub-sample regression to explore the variable health effects of patients with single and multiple diseases. A total of 1,055 patients with chronic diseases participated in the study, 54.4% of whom were women. The overall average utility score was 0.727, of which Sichuan Province was the highest with 0.751. Participants reported the highest proportion of pain/discomfort problems, while patients reported the least problems with self-care. The improvement of drug accessibility and the reduction of the burden of out-of-pocket expenses have significant positive effects on HRQoL. Various sociodemographic factors such as age and gender also have significant impact on HRQoL of patients with chronic diseases. HRQoL of patients with multiple chronic diseases is more affected by various influencing factors than that of patients with single disease. In order to improve the quality of life of patients with chronic diseases, it is of great importance to ensure the accessibility of drugs and reduce patients' medication burden. Future focus should shift from preventing and controlling chronic diseases as individual diseases to meeting the comprehensive health needs of people suffering from multiple diseases.
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http://dx.doi.org/10.3389/fpubh.2021.656104DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8006263PMC
March 2021

RhoA/Rock activation represents a new mechanism for inactivating Wnt/β-catenin signaling in the aging-associated bone loss.

Cell Regen 2021 Mar 3;10(1). Epub 2021 Mar 3.

Department of Pharmacology, Zhejiang University School of Medicine, 866 Yuhangtang Road, Hangzhou, 310058, China.

The Wnt/β-catenin signaling pathway appears to be particularly important for bone homeostasis, whereas nuclear accumulation of β-catenin requires the activation of Rac1, a member of the Rho small GTPase family. The aim of the present study was to investigate the role of RhoA/Rho kinase (Rock)-mediated Wnt/β-catenin signaling in the regulation of aging-associated bone loss. We find that Lrp5/6-dependent and Lrp5/6-independent RhoA/Rock activation by Wnt3a activates Jak1/2 to directly phosphorylate Gsk3β at Tyr216, resulting in Gsk3β activation and subsequent β-catenin destabilization. In line with these molecular events, RhoA loss- or gain-of-function in mouse embryonic limb bud ectoderms interacts genetically with Dkk1 gain-of-function to rescue the severe limb truncation phenotypes or to phenocopy the deletion of β-catenin, respectively. Likewise, RhoA loss-of-function in pre-osteoblasts robustly increases bone formation while gain-of-function decreases it. Importantly, high RhoA/Rock activity closely correlates with Jak and Gsk3β activities but inversely correlates with β-catenin signaling activity in bone marrow mesenchymal stromal cells from elderly male humans and mice, whereas systemic inhibition of Rock therefore activates the β-catenin signaling to antagonize aging-associated bone loss. Taken together, these results identify RhoA/Rock-dependent Gsk3β activation and subsequent β-catenin destabilization as a hitherto uncharacterized mechanism controlling limb outgrowth and bone homeostasis.
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http://dx.doi.org/10.1186/s13619-020-00071-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7925793PMC
March 2021

Investigating genetic diversity and population phylogeny of five Chongqing local chicken populations autosomal using microsatellites.

Anim Biotechnol 2021 Feb 26:1-19. Epub 2021 Feb 26.

College of Animal Science and Technology, Chongqing Key Laboratory of Forage and Herbivore, Chongqing Engineering Research Centre for Herbivores Resource Protection and Utilization, Southwest University, Chongqing, China.

The genetic diversity and population structures of five Chongqing local chicken populations were investigated using by 24 microsatellite markers. Results revealed that the mean number of alleles () ranged from 7.08 (Daninghe chicken, DN) to 8.46 (Nanchuan chicken, NC). The highest observed heterozygosity () and expected heterozygosity () were observed in DN ( = 0.7252; = 0.7409) and the lowest and were observed in XS (Xiushan native chicken [XS], = 0.5910 and = 0.6697). The inbreeding coefficient () within population ranged from 0.022 (DN) to 0.119 (XS). Among the 24 microsatellite markers, four loci (, , , and ) deviated from the Hardy-Weinberg equilibrium in all the studied populations. The results of population polygenetic analysis based on Nei's genetic distance and STRUCTURE software showed that the clustering of the five populations was incomplete consistent with geographical distribution. Moreover, a large number of gene flows were widespread among different populations, suggesting that genetic material exchanges occurred due to human activities and migration which was also verified by . In summary, this study preliminarily showed that Chongqing local chicken populations had rich genetic diversity and remarkable genetic divergence, but still high risk in conversion. These findings would be useful to the management of conservation strategies and the utilization of local chicken populations in further.
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http://dx.doi.org/10.1080/10495398.2021.1880421DOI Listing
February 2021

A case report of streptococcal toxic shock syndrome caused by Streptococcus mitis in a healthy adult.

BMC Infect Dis 2021 Feb 6;21(1):154. Epub 2021 Feb 6.

Department of Gynecology, Affiliated Hospital of Jining Medical University, N89 Guhuai Road, Jining, 272029, Shandong Province, China.

Background: Streptococcal toxic shock syndrome (STSS) is an acute, multisystem and toxin-mediated disease that usually causes shock and multiple organ failure in the early stages of its clinical course. It is associated with a substantial increase in mortality rate. The disease has been associated with invasive group A Streptococcus and is rarely caused by Streptococcus mitis (S. mitis). In healthy adults, S. mitis is closely related to endocarditis but rarely related to STSS.

Case Presentation: We report a case of STSS caused by S. mitis in a healthy 45-year-old woman. She presented with fever 14 h after surgery and with hypotension 24 h later, and she subsequently suffered from septic shock, low albumin, dysfunction of coagulation, acute kidney dysfunction, respiratory alkalosis and metabolic acidosis, acute respiratory distress syndrome and cellulitis of the incision. The diagnosis was obtained through clinical manifestation and blood culture examination. The patient was treated with aggressive fluid resuscitation, adequate antibiotics for a total of 4 weeks, respiratory support, and surgical debridement and drainage of the incision. She was discharged after her vital signs returned to normal and the incision healed on day 40 after surgery.

Conclusions: The diagnosis of STSS is often delayed or missed, which leads to a high mortality rate. It is possible to cure patients if the disease can be identified early and treated with aggressive fluid resuscitation, adequate antibiotics and control of the source of infection. Clinicians should consider the disease in the differential diagnosis of septic shock to prevent death.
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http://dx.doi.org/10.1186/s12879-021-05852-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7866958PMC
February 2021

Corrigendum to "A PLK1 kinase inhibitor enhances the chemosensitivity of cisplatin by inducing pyroptosis in oesophageal squamous cell carcinoma" [EBioMedicine 41 (2019) 244-255].

EBioMedicine 2021 Jan 6;63:103041. Epub 2021 Jan 6.

Key laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Laboratory of Molecular Oncology, Peking University Cancer Hospital & Institute, Beijing 100142, China. Electronic address:

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http://dx.doi.org/10.1016/j.ebiom.2020.103041DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7804599PMC
January 2021

Defect of SLC38A3 promotes epithelial-mesenchymal transition and predicts poor prognosis in esophageal squamous cell carcinoma.

Chin J Cancer Res 2020 Oct;32(5):547-563

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Laboratory of Molecular Oncology, Peking University Cancer Hospital & Institute, Beijing 100142, China.

Objective: Solute carrier family 38 (SLC38s) transporters play important roles in amino acid transportation and signaling transduction. However, their genetic alterations and biological roles in tumors are still largely unclear. This study aimed to elucidate the genetic signatures of SLC38s transporters and their implications in esophageal squamous cell carcinoma (ESCC).

Methods: Analyses on somatic mutation and copy number alterations (CNAs) of SLC38A3 were performed as described. Immunohistochemistry (IHC) assay and Western blot assay were used to detect the protein expression level. MTS assay, colony formation assay, transwell assay and wound healing assay were used to explore the malignant phenotypes of ESCC cells. Immunofluorescence assay was used to verify the colocalization of two indicated proteins and immunopreciptation assay was performed to confirm the interaction of proteins.

Results: Our findings revealed that SLC38s family was significantly disrupted in ESCC, with high frequent CNAs and few somatic mutations. was the most frequent loss gene among them and was linked to poor survival and lymph node metastasis. The expression of SLC38A3 was lower in tumor tissues compared to that in normal tissues, which was also significantly associated with worse clinical outcome. Further experiments revealed that depletion of SLC38A3 could promote EMT in ESCC cell lines, and the interaction of SLC38A3 and SETDB1 might lead to the reduced transcription of Snail. Pharmacogenomic analyses demonstrated that fifteen inhibitors were showed significantly correlated with SLC38A3 expression.

Conclusions: Our investigations have provided insights that SLC38A3 could act as a suppressor in EMT pathway and serve as a prognostic factor and predictor of differential drug sensitivities in ESCC.
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http://dx.doi.org/10.21147/j.issn.1000-9604.2020.05.01DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7666777PMC
October 2020

miR-100-3p inhibits the adipogenic differentiation of hMSCs by targeting PIK3R1 via the PI3K/AKT signaling pathway.

Aging (Albany NY) 2020 11 20;12(24):25090-25100. Epub 2020 Nov 20.

Key Laboratory of System Bio-Medicine of Jiangxi Province, Jiujiang University, Jiujiang 332000, China.

MicroRNAs play an important role in the adipogenic differentiation of human bone marrow mesenchymal stem cells (hMSCs). How miR-100-3p influences such adipogenesis, however, remains uncertain. In this study, hMSC adipogenic differentiation was associated with miR-100-3p downregulation, and overexpressing this miRNA inhibited adipogenesis and the expression of adipogenic marker genes. Through bioinformatics approaches, miR-100-3p can bind the 3'-untranslated region (3'-UTR) of the mRNA encoding phosphoinositide 3-kinase regulatory subunit 1 (PIK3R1) such that miR-100-3p overexpression resulted in significant reductions in PIK3R1 expression. Importantly, overexpressing PIK3R1 was sufficient to reverse the anti-adipogenic effects of miR-100-3p overexpression. PIK3R1 is a critical component of the PI3K/AKT signaling pathway, and miR-100-3p overexpression resulted in reduced AKT phosphorylation in the context of adipogenesis. In addition, the adipogenic differentiation of hMSCs in which miR-100-3p was overexpressed was further enhanced upon treatment with the PI3K/AKT agonist 740Y-P relative to miR-100-3p overexpression alone. Taken together, these findings provide evidence that miR-100-3p inhibits the adipogenic differentiation of hMSCs by targeting PIK3R1 via the PI3K/AKT signaling pathway.
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http://dx.doi.org/10.18632/aging.104074DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7803504PMC
November 2020

Measurement of the Equality of the Drug Welfare Induction Level of Chinese Patients With Chronic Diseases in Gansu, Sichuan, Hebei, and Zhejiang Based on the Bivariate Theil-T Index Method.

Front Public Health 2020 28;8:581533. Epub 2020 Oct 28.

School of Health Economics and Management, Nanjing University of Chinese Medicine, Nanjing, China.

This study aimed to measure the induction level of drug welfare in Chinese patients with chronic diseases using a bivariate Theil index. The bivariate Theil-T index was used to hierarchically decompose the relevant survey data, and the contribution rate of the intragroup gap and the intergroup gap to the total gap was investigated to better understand the current drug welfare induction level of Chinese patients with chronic diseases. The study was based in Gansu, Sichuan, Hebei, and Zhejiang provinces in China. Survey data was from patients with chronic diseases in 20 hospitals in four provinces. Data was collected through a questionnaire designed by the research team after expert consultation. Using the variables represented by the index system to decompose the Theil index from the two dimensions of the region and urban and rural areas. SPSS 22.0 was used for reliability and validity analysis and Theil index calculation. The overall level of drug welfare induction in Chinese patients with chronic diseases had a high degree of equalization. The overall Theil index was 0.0003, but there were still some differences among groups. To improve the drug welfare equalization induction level of patients with chronic diseases in China, the government should start from western rural areas, and policy should target the provinces that were in a disadvantaged position within the region to promote the equalization of drug welfare induction level for patients with chronic diseases in China.
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http://dx.doi.org/10.3389/fpubh.2020.581533DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7655781PMC
October 2020

HMGB1: A novel, potential therapeutic target in the treatment of cardiac fibrosis?

Int J Cardiol 2021 Jan 23;323:262. Epub 2020 Oct 23.

Department of Cardiology, Liaocheng People(,)s Hospital, Liaocheng 252000, PR China.. Electronic address:

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http://dx.doi.org/10.1016/j.ijcard.2020.10.058DOI Listing
January 2021

Relation of Gut Microbes and L-Thyroxine Through Altered Thyroxine Metabolism in Subclinical Hypothyroidism Subjects.

Front Cell Infect Microbiol 2020 18;10:495. Epub 2020 Sep 18.

Central Laboratory, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China.

Thyroxine metabolism is an important topic of pathogenesis research and treatment schedule of subclinical hypothyroidism (SCH). L-Thyroxine replacement therapy (LRT) is usually recommended for severe SCH patients only. Our previous studies reported that disordered serum lipid of mild SCH people could also benefit from LRT. However, the benefits were different among individuals, as shown by the variations in drug dosage that required to maintain thyroid-stimulating hormone (TSH) stability. Alternative pathways, such as sulfation and glucuronidation of iodothyronine, may play a role in thyroid hormones metabolism in peripheral tissues aside from thyroid. Conjugated thyroxine can be hydrolyzed and reused in tissues including gastrointestinal tract, in which gut microbiota are one of the most attractive physiological components. On this site, the roles of gut microbiota in thyroidal metabolism should be valued. In this study, a cross-sectional study was performed by analyzing 16S rDNA of gut microbiota in mild SCH patients treated with L-thyroxine or not. Subjects were divided by serum lipid level, L-thyroxine treatment, or L-thyroxine dosage, respectively. Relationship between gut microbiome and serum profile, L-thyroxine treatment, and dose were discussed. Other metabolic disorders such as type 2 diabetes and hypertension were also taken into consideration. It turned out that microbiome varied among individuals divided by dose and the increment of L-thyroxine but not by serum lipid profile. Relative abundance of certain species that were associated with thyroxine metabolism were found varied among different L-thyroxine doses although in relatively low abundance. Moreover, serum cholesterol may perform relevance effects with L-thyroxine in shaping microbiome. Our findings suggested that the differences in L-thyroxine dosage required to maintain TSH level stability, as well as the SCH development, which was displayed by the increased L-thyroxine doses in subsequent follow-up, had relationship with gut microbial composition. The reason may due to the differences in thyroxine metabolic capacity in gut. In addition, the metabolic similarity of iodothyronines and bile acid in gut also provides possibilities for the correlation between host's thyroxine and cholesterol levels. This study was registered with ClinicalTrials.gov as number NCT01848171.
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http://dx.doi.org/10.3389/fcimb.2020.00495DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7531258PMC
September 2020

Loganin inhibits macrophage M1 polarization and modulates sirt1/NF-κB signaling pathway to attenuate ulcerative colitis.

Bioengineered 2020 12;11(1):628-639

Department of Gastroenterology, The First Affiliated Hospital of Dalian Medical University , Dalian, People's Republic of China.

Loganin, a major bioactive iridoid glycoside derived from Cornus officinalis, exerts different beneficial biological properties. Recently, loganin has been reported to exhibit potential anti-inflammatory effects in the intestinal tissues, while the detailed mechanisms remain elusive. This study aimed to investigate whether loganin could inhibit the inflammatory response in dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) and to explore possible molecular mechanisms involved in this process. Results showed that oral administration of loganin significantly decreased body weight loss, disease activity index, colon shortening, myeloperoxidase (MPO) activity and pathologic abnormalities in UC mice. Loganin obviously inhibited the mRNA and protein levels of IL-6, TNF-α and IL-1β in colon tissues from UC mice. Furthermore, loganin remarkably reduced macrophage M1 polarization in UC mice evidenced by reduced the number of F4/80 and iNOS dual-stained M1 macrophages, and the expression of M1 macrophage-related pro-inflammatory chemokines/cytokines including MCP-1, CXCL10 as well as COX-2. Further investigation showed that loganin upregulated the mRNA and protein levels of Sirt1, with the inhibition of NF-κB-p65 acetylation in colon tissues from UC mice. Moreover, Sirt1-specific inhibitor Ex527 administration abolished the anti-inflammatory and anti-macrophage M1 polarization effects of loganin in UC. Thus, loganin could inhibit M1 macrophage-mediated inflammation and modulate Sirt1/NF-κB signaling pathway to attenuate DSS-induced UC. Loganin was considered as a viable natural strategy in the treatment of UC.[Figure: see text].
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http://dx.doi.org/10.1080/21655979.2020.1774992DOI Listing
December 2020

MicroRNA-936/ERBB4/Akt axis exhibits anticancer properties of gastric cancer through inhibition of cell proliferation, migration, and invasion.

Kaohsiung J Med Sci 2021 Feb 5;37(2):111-120. Epub 2020 Oct 5.

Department of Gastroenterology, The First Affiliated Hospital of Dalian Medical University, Dalian, China.

Gastric cancer is one of the most common cancers globally and has a poor prognosis. MiR-936 has been reported to regulate cell activity and tumor progression in non-small cell lung cancer, glioma, and epithelial ovarian cancer. However, the specific role and mechanism of miR-936 in gastric cancer have not been explored. In present study, gastric cancer cells were transfected with miR-936 mimic, and cell proliferation, cell cycle distribution, cell apoptosis, migration and invasion were assessed via cell-counting kit-8, flow cytometry, wound healing, and transwell assay, respectively. Dual luciferase reporter assay was used to check miR-936 binding to its downstream target. It was shown that miR-936 was downregulated in gastric cancer tissues and cells. Erb-B2 Receptor Tyrosine Kinase 4 (ERBB4) was confirmed as a direct target of miR-936 and negatively regulated its expression by miR-936. Overexpression of miR-936 suppressed cell proliferation, cell cycle progression, cell migration and invasion, and enhanced cell apoptosis in gastric cancer cells, which could be reversed by further ERBB4 overexpression. Western blot results showed that miR-936/ERBB4 axis regulated Akt-related pathways to control gastric cancer cell activities. Therefore, our data suggest that miR-936 overexpression inhibits cell proliferation and invasion and promotes cell apoptosis through Akt-related pathways by targeting ERBB4, which provides novel insight to target miR-936 or miR-936/ERBB4 axis for the treatment of gastric cancer.
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http://dx.doi.org/10.1002/kjm2.12304DOI Listing
February 2021

Differential diagnosis of pancreatoblastoma (PB) and solid pseudopapillary neoplasms (SPNs) in children by CT and MR imaging.

Eur Radiol 2021 Apr 30;31(4):2209-2217. Epub 2020 Sep 30.

Department of Radiology, Children's Hospital of Fudan University, 399 Wanyuan Rd., Shanghai, 200032, China.

Objectives: To determine whether features on computed tomographic and/or magnetic resonance imaging can differentiate pancreatoblastoma (PB) from solid pseudopapillary neoplasms (SPNs) of the pancreas in children.

Methods: Clinical and imaging data for 20 cases of SPNs and 14 cases of PB confirmed by surgery or biopsy were retrospectively analysed. The size, border, calcification, haemorrhage, solid/cystic component proportion, intratumoural vessels, tumour capsulation, pancreatic duct dilatation, peripancreatic vessel invasion, distant metastasis status and apparent diffusion coefficient (ADC) values of the two groups were examined, and key diagnostic features were identified. Statistical analysis was performed using the chi-square test and Student's t test. Sensitivity and specificity values were calculated when a single criterion was used.

Results: Age ≤ 5 years, elevated serum α-fetoprotein (AFP), larger size, ill-defined border, calcification, absence of haemorrhage, intratumoural vessel, peripancreatic vessel invasion and distant metastasis differentiated PB from SPN (p < 0.05). ADC values of SPN were higher than those of PB (p = 0.001). There were no significant differences regarding tumour capsule (p = 0.435), pancreatic duct dilatation (p = 1.000) or cystic degeneration area over 50% of the tumour volume (p = 1.000) between the two groups.

Conclusions: The following features are helpful for differentiating PB from SPN: age ≤ 5 years, elevated serum AFP, larger size, ill-defined border, calcification, haemorrhage absence, intratumoural vessel, peripancreatic vessel invasion, distant metastasis and lower ADC value.

Key Points: • CT and MRI are helpful to differentiate pancreatoblastoma (PB) from solid pseudopapillary neoplasms (SPNs) of the pancreas in children. • The following features are helpful to differentiate PB from SPN: age ≤ 5 years, elevated serum AFP, larger size, ill-defined border, calcification, absence of haemorrhage, intratumoural vessel, peripancreatic vessel invasion, distant metastasis and lower ADC value.
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http://dx.doi.org/10.1007/s00330-020-07309-3DOI Listing
April 2021

Both environmental and spatial variables affect bacterial functional diversity in mangrove sediments at an island scale.

Sci Total Environ 2021 Jan 31;753:142054. Epub 2020 Aug 31.

State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, People's Republic of China; School of Biology, Hainan Normal University, Haikou 571158, People's Republic of China. Electronic address:

Sediment microorganisms are influenced by various biotic and abiotic factors. However, information concerning the spatial factors that determine the functional diversity of sediment bacterial communities at an island scale is limited. Here, we conducted an island-scale study to assess the driving forces governing the functional diversity of sediment bacterial communities in different mangroves around the coast of Hainan Island, southern China. For mangrove sediments in Hainan Island, differences in the metabolic activity and functional diversity among four sites were context dependent, while that showed a trend of East > North > West > South. Furthermore, total carbon, nitrite nitrogen, and salinity are important environmental factors that determine the metabolic functional diversity of bacterial communities. This study also provided important insights for explaining the metabolic functional diversity of bacterial communities in tropical mangrove sediments. The metabolic activity had a significantly response to environmental variables (13.2% of pure variance was explained) and spatial variables (12.4%). More importantly, given that spatial variables may contribute to the bacterial functional as important as environmental variables, this spatial variety of bacterial functional provides new insight into studying bacterial functional biogeographic patterns and impacts on sediment-associated function.
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http://dx.doi.org/10.1016/j.scitotenv.2020.142054DOI Listing
January 2021

Improved Dielectric Properties of Thermoplastic Polyurethane Elastomer Filled with Core-Shell Structured PDA@TiC Particles.

Materials (Basel) 2020 Jul 27;13(15). Epub 2020 Jul 27.

School of Chemistry and Chemical Engineering, Xi'an University of Science and Technology, Xi'an 710054, China.

Insulating interlayer between nanoparticles and polymer matrix is crucial for suppressing the dielectric loss of polymer composites. In this study, titanium carbide (TiC) particles were surface modified by polydopamine (PDA), and the obtained PDA@TiC powders were used to reinforce thermoplastic polyurethane (TPU). The results indicate that the PDA@TiC were homogenously dispersed in the matrix compared with the pristine TiC, and that the PDA@TiC/TPU composites show improved dielectric and mechanical properties, i.e., much lower dissipation factors and obviously enhanced dielectric breakdown strength, as well as higher tensile strength and elongation at break as compared to the raw TiC/TPU. The nanoscale PDA interlayer contributes to the dielectric and mechanical enhancements because it not only serves as an insulating shell that prevents TiC particles from direct contacting and suppresses the loss and leakage current to very low levels, but also enhances the interfacial interactions thereby leading to improved mechanical strength and toughness. The prepared flexible PDA@TiC/TPU with high permittivity but low loss will find potential applications in electronic and electrical applications.
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http://dx.doi.org/10.3390/ma13153341DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7435405PMC
July 2020

[Expert consensus on clinical application of Chinese herbal medicine decoction pieces (First Edition)].

Zhongguo Zhong Yao Za Zhi 2020 Jul;45(13):3238-3244

Dongfang Hospital, Beijing University of Chinese Medicine Beijing 100078, China.

Chinese herbal medicine decoction pieces(CHMDP), one of the main forms of traditional Chinese medicine(TCM) in clinic, have been widely used. However, the irrational use is increasingly serious due to the lack of the indicators for judging the rational use of CHMDP in medical institutions and the codes and standards for the clinical use of CHMDP. In order to regulate the rational clinical use of CHMDP, improve the clinical efficacy and ensure the drug safety for the patients, clinical pharmaceutical experts and clinical medical experts from 40 third-grade class-A hospitals nationwide were organized to give the "expert consensus on clinical application of CHMDP" in terms of prescription writing, combined use of drugs, use of special drugs, and drug use for special population. Detailed analysis and argumentation were conducted in accordance with the laws and regulations, Chinese Pharmacopoeia 2015 edition, Chinese Pharmacopoeia Code Notice for Clinical Use of Medicine, Administrative Regulations for Prescriptions, Administrative Specifications for Hospital Prescription Review(interim), and Chinese Traditional Medicine Prescription Format and Writing Specifications, as well as relevant project findings.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20200424.308DOI Listing
July 2020

Characterization of a Novel Chitinase from Sweet Potato and Its Fungicidal Effect against .

J Agric Food Chem 2020 Jul 8;68(29):7591-7600. Epub 2020 Jul 8.

Jiangsu Key Laboratory of Phylogenomics and Comparative Genomics, School of Life Sciences, Jiangsu Normal University, Xuzhou, Jiangsu Province 221116, China.

Black rot, caused by , is a destructive disease of sweet potatoes (). In this study, a novel chitinase () was screened from sweet potatoes, which showed a remarkably higher expression level in resistant varieties than in susceptible ones after inoculation with . Sequence analysis indicated that IbChiA belongs to family 19 class II extracellular chitinase with a MW of 26.3 kDa and pI of 5.96. Recombinant IbChiA, produced by , displayed antifungal activity and stability. IbChiA could restrain the mycelium extension of . FDA/PI double staining combined with transmission electron microscopy observation revealed the remarkable fungicidal effect of IbChiA on the conidia of . The disease symptoms on the surface of slices and tuberous roots of sweet potatoes were significantly reduced after treatment with IbChiA. These results indicated that IbChiA could be used as a potential biofungicide to replace chemical fungicides.
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http://dx.doi.org/10.1021/acs.jafc.0c01813DOI Listing
July 2020

Cut the weeds and dig up the roots: clip-and-snare assisted endoscopic mucosal resection of a rectal neuroendocrine tumor.

Endoscopy 2021 Jan 29;53(1):E13-E14. Epub 2020 May 29.

Department of Gastroenterology, The First Affiliated Hospital, Dalian Medical University, Dalian, Liaoning, China.

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http://dx.doi.org/10.1055/a-1163-7140DOI Listing
January 2021

PFKP is transcriptionally repressed by BRCA1/ZBRK1 and predicts prognosis in breast cancer.

PLoS One 2020 29;15(5):e0233750. Epub 2020 May 29.

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Laboratory of Molecular Oncology, Peking University Cancer Hospital & Institute, Beijing, China.

Objectives: The present study aims to elucidate the underlying mechanism how PFKP is regulated by BRCA1 and the clinical significance of PFKP in breast cancer.

Methods: MEF-BRCA1△/△ and the wild type counterpart MEF-BRCA1+/+ cell lines were used to test the sensitivity of glucose depletion in culture medium. Glucose Assay Kit was used to quantify glucose levels in cultural supernatant and cell lysate. Real time PCR was used to measure the mRNA expression levels of genes. Western blot was used to detect protein levels. Chromatin immunoprecipitation was used to verify the bindings between transcription factors and DNA elements. Luciferase reporter assay was performed to determine the transcriptional activity. Histochemistry assay was performed on tissue microarray.

Results: We found that MEF-BRCA1△/△ cells consumed more glucose and were more vulnerable to glucose-deprived culture medium. The mRNA profiles and qPCR assay of MEF-BRCA1△/△ and MEF-BRCA1+/+ cells revealed that PFKP, the rate-limiting enzyme of glycolysis, was significantly upregulated in MEF-BRCA1△/△ cells. Consistently, the repressive effects of BRCA1 on PFKP were confirmed by overexpression or knockdown of BRCA1. Moreover, we also demonstrated that PFKP was suppressed by ZBRK1 as well, which was the co-repression partner of BRCA1. Mechanistically, we figured out that BRCA1 formed a transcriptional repression complex with ZBRK1 on the promoter of PFKP and consequently restrained its expression. Importantly, the expression levels of PFKP were demonstrated to associate with poor survival of patients with breast cancer.

Conclusion: Our study provided a new insight into the dysregulation of glycolysis in breast cancer, which might be partially due to the deficiency of BRCA1/ZBRK1 axis and subsequently reversed the transcriptional repressive effect on PFKP. We also found that PFKP overexpressed in a subset of breast cancer patients and could serve as a prognostic factor, which represented a potential target for BC therapy.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0233750PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7259711PMC
September 2020

Rosuvastatin Enhances VSV-G Lentiviral Transduction of NK Cells via Upregulation of the Low-Density Lipoprotein Receptor.

Mol Ther Methods Clin Dev 2020 Jun 29;17:634-646. Epub 2020 Mar 29.

Department of Internal Medicine, Division of Hematology, Maastricht University Medical Center+, Maastricht, the Netherlands.

Adoptive natural killer (NK) cell therapy is attaining promising clinical outcomes in recent years, but improvements are needed. Genetic modification of NK cells with a tumor antigen-specific receptor on their surface coupled to intracellular signaling domains may lead to enhanced cytotoxicity against malignant cells. One of the most common approaches is by lentivirus-mediated transduction. However, NK cells are difficult to transduce and various methods have been attempted with different success rates. Because the low-density lipoprotein-receptor (LDLR) is the receptor of vesicular stomatitis virus (VSV) and is expressed only at low levels on NK cells, we tested the potential of 5 statins and 5 non-statin compounds to increase the LDLR expression, thereby facilitating viral transduction. We found that the transduction efficiency of VSV-G pseudotyped lentivirus is augmented by statins that induced higher LDLR expression. In both NK-92 cells and primary NK cells, the transduction efficiency increased after treatment with statins. Furthermore, statins have been reported to suppress NK cell cytotoxicity; however, we showed that this can be completely reversed by adding geranylgeranyl-pyrophosphate (GGPP). Among the statins tested, we found that the combination of rosuvastatin with GGPP most potently improved viral transduction without affecting the cytotoxic properties of the NK cells.
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http://dx.doi.org/10.1016/j.omtm.2020.03.017DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7150439PMC
June 2020

and activity of chelerythrine against and underlying mechanisms.

Future Microbiol 2019 12 29;14:1545-1557. Epub 2020 Jan 29.

Department of Pharmacy, Shandong Provincial Qianfoshan Hospital, The First Hospital Affiliated with Shandong First Medical University, Jinan, 250014, PR China.

To evaluate whether chelerythrine (CHT) exhibited antifungal activity against and and to explore the underlying mechanisms. Broth microdilution assay and model were used to evaluate the antifungal effect and , respectively. Mechanism studies were investigated by morphogenesis observation, Fluo-3/AM, DCFH-DA and rhodamine6G assay, respectively. CHT exhibited antifungal activity against and preformed biofilms with minimum inhibitory concentrations ranged from 2 to 16 μg/ml. Besides, CHT protected larvae infected by . Mechanisms studies revealed that CHT inhibited hyphal growth, increased intracellular calcium concentration, induced accumulation of reactive oxygen species and inhibited drug transporter activity. CHT exhibited antifungal activity against .
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http://dx.doi.org/10.2217/fmb-2019-0178DOI Listing
December 2019

Complications of enterostomy and related risk factor analysis of very early onset inflammatory bowel disease with interleukin-10 signalling deficiency: a single-centre retrospective analysis.

BMC Gastroenterol 2020 Jan 13;20(1). Epub 2020 Jan 13.

Department of Gastroenterology, Children's Hospital of Fudan University, 399 Wanyuan Road, Minhang District, Shanghai, 201102, People's Republic of China.

Background: Interleukin-10 (IL10) signalling pathway deficiency results in severe very early onset inflammatory bowel disease (VEOIBD), and enterostomy is often inevitable. However, studies in these surgical populations are lacking. This study aims to determine the enterostomy characteristics, postoperative complications and related risk factors in enterostomy patients.

Methods: From March 1, 2015, to December 31, 2018, patients with IL10R-mutation who underwent enterostomy were recruited for analysis. We collected data on the patients' clinical characteristics, enterostomy characteristics, postoperative complications and related risk factors.

Results: Twelve patients required emergency enterostomy, and 10 patients underwent elective enterostomy. Twelve patients experienced postoperative complications, including wound infection (27.3%), wound dehiscence (18.2%), reoperation (18.2%), etc. Compared with the pre-enterostomy values, there was a decrease in C-reactive protein (CRP) (P = 0.001), an increase in albumin (P = 0.001) and an improvement in the weight-for-age (P = 0.029) and body mass index (BMI) Z-scores (P = 0.004) after enterostomy. There was a significant difference between the pre-operation and postoperation medicine expenses (P = 0.002). Univariate binary logistic regression analysis revealed a statistically significant influence of CRP (OR: 1.43, 95% CI: 1.07-1.91, P = 0.016) and a tendency towards a significant influence of intestinal perforation, albumin level, BMI Z-score and weighted paediatric Crohn's disease activity index (wPCDAI). Multivariate logistic regression analysis showed that CRP (OR: 1.40), wPCDAI (OR: 2.88) and perforation (OR: 1.72) showed a tendency to behave as independent risk factors for postoperative complications, but the results were not significant (all P > 0.05).

Conclusions: Surgery and enterostomy showed benefits for VEOIBD with IL-10 signalling deficiency. The timing of intervention, potential postoperative complications, economic burden and other related problems should be considered.
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http://dx.doi.org/10.1186/s12876-020-1160-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6958732PMC
January 2020

The Synergistic Antifungal Effect and Potential Mechanism of D-Penicillamine Combined With Fluconazole Against .

Front Microbiol 2019 18;10:2853. Epub 2019 Dec 18.

Department of Pharmacy, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, China.

Over the last few decades, candidiasis has exhibited an increasing incidence worldwide, causing high mortality in immunocompromised patients. is one of the leading opportunistic fungal pathogens. However, due to the increased use of antifungal agents, resistance of to conventional agents, especially fluconazole, has frequently emerged. Therefore, research on the use of combinations of current drugs to sensitize antifungal agents and overcome fungal resistance has attracted considerable attention. This study demonstrated for the first time that D-penicillamine (PCA) combined with fluconazole showed a synergistic effect against . PCA combined with fluconazole not only showed synergistic effects against planktonic cells of , but also showed synergistic effects against biofilms formed within 12 h . In addition, a infection model was used to evaluate the effects of this drug combination. The results showed that the combination of the two drugs could improve the survival rate, decrease the fungal burden, and reduce the tissue invasion of larvae. Finally, we explored the potential synergistic mechanisms of the drug combination, mainly including inhibition of the morphological transformation, reduction of the intracellular calcium concentration, and the activation of metacaspase, which is closely related to cell apoptosis. These findings might provide novel insights into antifungal drug discovery and the treatment of candidiasis caused by .
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http://dx.doi.org/10.3389/fmicb.2019.02853DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6930176PMC
December 2019

Occurrence and potential health risks assessment of polycyclic aromatic hydrocarbons (PAHs) in different tissues of bivalves from Hainan Island, China.

Food Chem Toxicol 2020 Feb 31;136:111108. Epub 2019 Dec 31.

College of Ecology and Environment, Hainan University, Haikou, 570228, China; State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, 570228, China; School of Biology, Hainan Normal University, Haikou, 571158, China. Electronic address:

The levels of 16 PAHs were determined in the adductor, gills, gonads, hepatopancreas and mantles of the pearl oyster (Pinctada martensii) and the mussel (Perna viridis) collected from coasts of Li'an and Xincun Bays. The levels of ΣPAHs ranged from 597.1 to 2332 ng g d w in the various tissues of bivalves. The pyrolytic source played an important role in the local coastal environment. Significantly higher levels of M-PAHs and H-PAHs were observed in Pinctada martensii than in Perna viridis. The ΣPAHs at different tissues showed the following order from high to low: mantles > hepatopancreas > gonads > gills > adductor. When levels of individual PAHs in the five bivalve tissues have been compared with each other, high correlations have been found (r = 0.793-0.975). A general trend was observed that log transformed BSAFs declined with increase of K values. The estimated amount of ΣPAHs via ingestion of oyster and mussel varied from 1.35 × 10-1.70 × 10 and 2.15 × 10-1.91 × 10 μg kg body weight day, respectively. The THQs and CRs calculated for regular consumption of raw bivalves were in the acceptable ranges and may not pose health risk concerns. But for certain population with higher consumption rate for PAHs contaminated bivalves, cautions should be taken for their higher cancer risk.
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http://dx.doi.org/10.1016/j.fct.2019.111108DOI Listing
February 2020

Renal artery assessment with non-enhanced MR angiography versus digital subtraction angiography: comparison between 1.5 and 3.0 T.

Eur Radiol 2020 Mar 3;30(3):1747-1754. Epub 2019 Dec 3.

Department of Radiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.

Objectives: To compare non-enhanced magnetic resonance angiography (NE-MRA) between 1.5 and 3.0-T using a balanced steady-state free precession (bSSFP) sequence in the assessment of renal artery stenosis (RAS) with digital subtraction angiography (DSA) as a reference standard.

Methods: From March 2016 to May 2018, 81 patients suspected to have significant RAS were scheduled for DSA. All patients underwent NE-MRA at either 1.5 T or 3.0 T randomly before DSA. In total, 49 patients underwent 1.5-T NE-MRA, and 32 patients underwent 3.0-T NE-MRA. Image quality was assessed. Degree of stenosis evaluated with NE-MRA was compared with that with DSA.

Results: NE-MRA provided excellent image qualities for segment 1 and segment 2 at 1.5 T and 3.0 T. Image qualities for segment 3 and segment 4 and the degree of renal artery branches were significantly higher at 3.0 T than at 1.5 T (p < 0.01). Stenoses evaluated with NE-MRA at 1.5 T (r = 0.853, p < 0.01) and 3.0 T (r = 0.811, p < 0.01) were highly correlated with those of DSA. The Bland-Altman plots showed overestimated degrees of stenosis at 1.5 T (mean bias, 3.5% ± 20.4) and 3.0 T (mean bias, 8.4% ± 21.7). The sensitivity and specificity for significant stenosis were 97.4% and 89.8% for 1.5 T and 95.7% and 91.1% for 3.0 T.

Conclusions: Both 1.5-T and 3.0-T bSSFP NE-MRA can reliably assess RAS, with high image quality and good diagnostic accuracy. Performing NE-MRA at 3.0 T significantly improved visualization of renal artery branches but showed greater tendency to overestimate stenosis compared with that at 1.5 T.

Key Points: • Both 1.5-T and 3.0-T NE-MRA provide excellent image quality and good diagnostic accuracy for RAS. • NE-MRA at 3.0 T improved visualization of renal artery branches compared with that at 1.5 T.
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http://dx.doi.org/10.1007/s00330-019-06440-0DOI Listing
March 2020

Capsaicin metabolites and GSH-associated detoxification and biotransformation pathways in human liver microsomes revealed by LC-HRMS/MS with data-mining tools.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 Dec 21;1133:121843. Epub 2019 Oct 21.

Laboratory of Toxicant Analysis, Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, and State Key Laboratory of Toxicology and Medical Countermeasures, Beijing 100850, China.

Capsaicin (CAP) is a principal pungent ingredient in hot peppers, it is also employed as a common food additive, an efficient pharmaceutical component, or even a riot control agent. CAP exerts various pharmacological activities as well as associated adverse physiological responses and causes moderate toxicity if overused. A full screening and identification of CAP metabolites in combination with its main detoxification pathways are crucial for the clear demonstration on its pharmacological and toxicological significance. Here, we employed a post-acquisition data-mining metabolic screening approach to rapidly find and identify a broad range of CAP metabolites generated from in vitro human liver microsomes, based on an ultra-performance liquid chromatography-quadrupole orbitrap high resolution tandem mass spectrometric method. First, we collected full scan MS and MS/MS data sets by a data-dependent acquisition method in positive ion mode, and then we employed a modified mass defect filter and a diagnostic ion filter to screen and identify all the probable CAP metabolites, combining with information including retention time, accurate mass, characteristic fragments, and relevant drug biotransformation patterns. In comparison with the stable isotope-labeled CAP involved biotransformation products, we confirmed 19 functionalized metabolites and 13 glutathione (GSH) conjugates of CAP, in which 13 metabolites are reported for the first time. We then briefly depicted an overview metabolic pathway of CAP from the GSH detoxification viewpoint, revealed that various metabolites of CAP can be generated from single or multiple biotransformation and metabolic reactions. Both CAP and its reactive metabolites produced relevant GSH conjugates, which indicates a wide and important detoxification value of GSH conjugation way.
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http://dx.doi.org/10.1016/j.jchromb.2019.121843DOI Listing
December 2019

Long-term outcomes of total hip arthroplasty in patients younger than 55 years: a systematic review of the contemporary literature

Can J Surg 2019 08;62(4):249-258

From the Department of Orthopaedic Surgery, Mount Sinai Hospital, Toronto, Ont. (Mei, Safir, Gross, Kuzyk); and the Department of Family Medicine, Queen’s University, Kingston, Ont. (Gong).

Background: Total hip arthroplasty (THA) is increasingly performed in younger patients despite the lack of comprehensive assessment of long-term outcomes. We systematically reviewed the contemporary literature to assess the 1) indications, 2) implant selection and long-term survivorship, 3) complication and reoperation rates and 4) radiographic and functional outcomes of primary THA in patients younger than 55 years.

Methods: We searched the Embase and MEDLINE databases for English-language articles published between 2000 and 2018 that reported outcomes of primary THA in patients younger than 55 years with a minimum follow-up duration of 10 years.

Results: Thirty-two studies reporting on 3219 THA procedures performed in 2434 patients met our inclusion criteria. The most common preoperative diagnoses were avascular necrosis (1044 [32.4%]), osteoarthritis (870 [27.0%]) and developmental dysplasia of the hip (627 [19.5%]). Modular implants (3001 [93.2%]), cementless fixation (2214 [68.8%]) and metal-on-polyethylene bearings (1792 [55.7%]) were frequently used. The mean 5- and 10-year survival rates were 98.7% and 94.6%, respectively. Data on survival beyond 10 years were heterogeneous, with values of 27%–99.5% at 10–14 years, 59%–84% at 15–19 years, 70%–77% at 20–24 years and 60% at 25–30 years. Rates of dislocation, deep infection and reoperation for any reason were 2.4%, 1.2% and 16.3%, respectively. The mean Harris Hip Score improved from 43.6/100 to 91.0/100.

Conclusion: Total hip arthroplasty in patients younger than 55 years provides reliable outcomes at up to 10 years. Future studies should evaluate the outcomes of THA in this population at 15–20 years’ follow-up.
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http://dx.doi.org/10.1503/cjs.013118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6660275PMC
August 2019

Antifungal Activity and Potential Mechanism of N-Butylphthalide Alone and in Combination With Fluconazole Against .

Front Microbiol 2019 2;10:1461. Epub 2019 Jul 2.

Department of Pharmacy, Shandong Provincial Qianfoshan Hospital, The First Hospital Affiliated with Shandong First University, Jinan, China.

is a common opportunistic fungal pathogen that may cause nosocomial fungal infections. The resistance of to traditional antifungal drugs has been increasing rapidly in recent years, and it brings a great challenge in clinical treatment. N-butylphthalide is originally extracted from the seed of and is currently used for the treatment of ischemic stroke in the clinic. This study demonstrated that n-butylphthalide exhibited antifungal activity against with minimum inhibitory concentrations of 128 μg/ml; moreover, n-butylphthalide combined with fluconazole showed synergistic antifungal effects against resistant , resulting in a decrease in the minimum inhibitory concentrations of fluconazole from >512 to 0.25-1 μg/ml. Time-killing curves verified the antifungal activity in dynamic. Besides, n-butylphthalide exhibited anti-biofilm activity against , biofilms preformed <12 h with sessile minimum inhibitory concentrations of 128-256 μg/ml and synergism was observed when n-butylphthalide combined with fluconazole against resistant biofilms preformed <12 h, resulting in a decrease in the sessile minimum inhibitory concentrations of fluconazole from >1,024 to 0.5-8 μg/ml. Furthermore, antifungal effects of n-butylphthalide were confirmed . N-butylphthalide prolonged survival rate of larvae infected by , reduced the fungal burden in larvae and caused less damage to larval tissues. Notably, n-butylphthalide inhibited hyphal growth and induced intracellular reactive oxygen species accumulation and a loss in mitochondrial membrane potential, which was a potential antifungal mechanism. Besides, the synergistic effects between n-butylphthalide and fluconazole potentially relied on the mechanism that n-butylphthalide significantly promoted drug uptake, and suppressed drug efflux down-regulating the drug transporter encoding genes and . These findings demonstrated the antifungal effects and mechanisms of n-butylphthalide against for the first time, which might provide broad prospects for the identification of new potential antifungal targets.
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http://dx.doi.org/10.3389/fmicb.2019.01461DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6614440PMC
July 2019