Publications by authors named "Ying Gao"

1,397 Publications

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DFTNet: Dual-Path Feature Transfer Network for Weakly Supervised Medical Image Segmentation.

IEEE/ACM Trans Comput Biol Bioinform 2022 Aug 11;PP. Epub 2022 Aug 11.

Medical image segmentation has long suffered from the problem of expensive labels. Acquiring pixel-level annotations is time-consuming, labor-intensive, and relies on extensive expert knowledge. Bounding box annotations, in contrast, are relatively easy to acquire. Thus, in this paper, we explore to segment images through a novel Dual-path Feature Transfer design with only bounding box annotations. Specifically, a Target-aware Reconstructor is proposed to extract target-related features by reconstructing the pixels within the bounding box through the channel and spatial attention module. Then, a sliding Feature Fusion and Transfer Module (FFTM) fuses the extracted features from Reconstructor and transfers them to guide the Segmentor for segmentation. Finally, we present the Confidence Ranking Loss (CRLoss) which dynamically assigns weights to the loss of each pixel based on the network's confidence. CRLoss mitigates the impact of inaccurate pseudo-labels on performance. Extensive experiments demonstrate that our proposed model achieves state-of-the-art performance on the Medical Segmentation Decathlon (MSD) Brain Tumour and PROMISE12 datasets.
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http://dx.doi.org/10.1109/TCBB.2022.3198284DOI Listing
August 2022

Effect of vitamin E and supragingival scaling on salivary gland function in patients with differentiated thyroid cancer treated with 131I.

Nucl Med Commun 2022 Sep 10;43(9):995-1003. Epub 2022 Aug 10.

Department of Stomatology.

Objective: The aim of this study was to evaluate the effect of vitamin E and supragingival scaling with vitamin C on the salivary glands of patients with differentiated thyroid carcinoma after 131I treatment.

Methods: A total of 89 prospective patients with differentiated thyroid carcinoma were enrolled and randomly divided into the following groups: vitamin E group (n = 30, group A), vitamin C group (n = 30, group B) and supragingival scaling with vitamin C group (n = 29, group C). Using functional indices (e.g. maximum uptake fraction, uptake index, excretion fraction, secretion time and excretion rate), changes in the salivary gland functions before and a month after 131I treatment were assessed by dynamic imaging of salivary gland.

Results: We compared the before and after 131I therapy results of the three groups. In group A (P  < 0.05), the excretion fraction and excretion rate of the left parotid gland were significantly higher, and the uptake index of the bilateral submandibular glands was significantly lower. No significant changes in salivary gland functional parameters were observed in group B (P > 0.05). The uptake index of the bilateral parotid glands and the excretion rate of the left parotid gland were significantly higher in group C (P < 0.05). The degree of serum amylase level reduction decreased significantly in group C (P < 0.05).

Conclusion: Vitamin E showed a protective effect on parotid excretion function in patients with differentiated thyroid carcinoma who underwent 131I treatment. Supragingival scaling may be a promising radiation protector because it is associated with a protective effect on the salivary gland functions.
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http://dx.doi.org/10.1097/MNM.0000000000001605DOI Listing
September 2022

Effect of vitamin E and supragingival scaling on salivary gland function in patients with differentiated thyroid cancer treated with 131I.

Nucl Med Commun 2022 Aug 5. Epub 2022 Aug 5.

Department of Stomatology Shanxi Medical University School and Hospital of Stomatology, Taiyuan.

Objective: The aim of this study was to evaluate the effect of vitamin E and supragingival scaling with vitamin C on the salivary glands of patients with differentiated thyroid carcinoma after 131I treatment.

Methods: A total of 89 prospective patients with differentiated thyroid carcinoma were enrolled and randomly divided into the following groups: vitamin E group (n = 30, group A), vitamin C group (n = 30, group B) and supragingival scaling with vitamin C group (n = 29, group C). Using functional indices (e.g. maximum uptake fraction, uptake index, excretion fraction, secretion time and excretion rate), changes in the salivary gland functions before and a month after 131I treatment were assessed by dynamic imaging of salivary gland.

Results: We compared the before and after 131I therapy results of the three groups. In group A (P  < 0.05), the excretion fraction and excretion rate of the left parotid gland were significantly higher, and the uptake index of the bilateral submandibular glands was significantly lower. No significant changes in salivary gland functional parameters were observed in group B (P > 0.05). The uptake index of the bilateral parotid glands and the excretion rate of the left parotid gland were significantly higher in group C (P < 0.05). The degree of serum amylase level reduction decreased significantly in group C (P < 0.05).

Conclusion: Vitamin E showed a protective effect on parotid excretion function in patients with differentiated thyroid carcinoma who underwent 131I treatment. Supragingival scaling may be a promising radiation protector because it is associated with a protective effect on the salivary gland functions.
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http://dx.doi.org/10.1097/MNM.0000000000001605DOI Listing
August 2022

Spatiotemporal evolution of pyroptosis and canonical inflammasome pathway in hSOD1 ALS mouse model.

BMC Neurosci 2022 Aug 9;23(1):50. Epub 2022 Aug 9.

Department of Neurosurgery, Brigham and Women's Hospital, Harvard Medical School, 221 Longwood Ave, Boston, MA, 02115, USA.

Background: Evidences indicate that inflammasome compounds participate in amyotrophic lateral sclerosis (ALS), a fatal progressive motoneuron degenerative disease. Researchers have observed the expressions of nucleotide oligomerization domain (NOD)-like receptor protein 3 (NLRP3) related inflammasome components in specific regions of the central nervous system in different ALS models, but the cellular spatiotemporal evolution of this canonical inflammasome pathway and pyroptosis during ALS progression are unclear.

Methods: The spinal cords of hSOD1 mice (ALS mice) and age-matched littermates (CON mice) were dissected at pre-symptomatic stage (60 d), early- symptomatic stage (95 d), symptomatic stage (108 d) and late-symptomatic stage (122 d) of the disease. By using Nissl staining, double immunofluorescence labelling, qRT-PCR or western blot, we detected morphology change and the expression, cellular location of GSDMD, NLRP3, caspase-1 and IL-1β in the ventral horn of lumbar spinal cords over the course of disease.

Results: Neural morphology changes and GSDMD/NeuN double positive cells were observed in ventral horn from ALS mice even at 60 d of age, even though there were no changes of GSDMD mRNA and protein expressions at this stage compared with CON mice. With disease progression, compared with age-matched CON mice, increased expressions of GSDMD, NLRP3, activated caspase-1 and IL-1β were detected. Double immunofluorescence labeling revealed that NLRP3, caspase-1, IL-1β positive signals mainly localized in ventral horn neurons at pre- and early-symptomatic stages. From symptomatic stage to late-symptomatic stage, robust positive signals were co-expressed in reactive astrocytes and microglia.

Conclusions: Early activation of the canonical NLRP3 inflammasome induced pyroptosis in ventral horn neurons, which may participate in motor neuron degeneration and initiate neuroinflammatory processes during ALS progression.
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http://dx.doi.org/10.1186/s12868-022-00733-9DOI Listing
August 2022

DMCGNet: A Novel Network for Medical Image Segmentation with Dense Self-Mimic and Channel Grouping Mechanism.

IEEE J Biomed Health Inform 2022 Aug 8;PP. Epub 2022 Aug 8.

Automatic Medical Image Segmentation (MIS) can assist doctors by reducing labor and providing a unified standard. Nowadays, approaches based on Deep Learning have become mainstream for MIS because of their ability of automatic feature extraction. However, due to the plain network design and targets variety in medical images, the semantic features can hardly be extracted adequately. In this work, we propose a novel Dense Self-Mimic and Channel Grouping based Network (DMCGNet) for MIS for better feature extraction. Specifically, we introduce a Pyramid Target-aware Dense Self Mimic (PTDSM) module, which is capable of exploring deeper and better feature representation with no parameter increase. Then, to utilize features efficiently, an effective Channel Split based Feature Fusion Module (CSFFM) is proposed for feature reuse, which strengthens the adaptation of multi-scale targets by utilizing the channel grouping mechanism. Finally, to train the proposed method adequately, Deep Supervision with Group Ensemble Learning (DSGEL) is equipped to the network. Extensive experiments demonstrate that our proposed model achieves state-of-the-art performance on 4 medical image segmentation datasets.
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http://dx.doi.org/10.1109/JBHI.2022.3192277DOI Listing
August 2022

Cuproptosis-Related lncRNAs are Biomarkers of Prognosis and Immune Microenvironment in Head and Neck Squamous Cell Carcinoma.

Front Genet 2022 22;13:947551. Epub 2022 Jul 22.

Department of Prosthodontics, Hospital of Stomatology, Jilin University, Changchun, China.

Cuproptosis is a new type of cell death that induces protein toxic stress and eventually leads to cell death. Hence, regulating cuproptosis in tumor cells is a new therapeutic approach. However, studies on cuproptosis-related long noncoding RNA (lncRNA) in head and neck squamous cell carcinoma (HNSC) have not been found. This study aimed to explore the cuproptosis-related lncRNAs prognostic marker and their relationship to immune microenvironment in HNSC by using bioinformatics methods. RNA sequencing, genomic mutations, and clinical data of TCGA_HNSC were downloaded from The Cancer Genome Atlas. HNSC patients were randomly assigned to either a training group or a validation cohort. The least absolute shrinkage and selection operator Cox regression and multivariate Cox regression models were used to determine the prognostic model in the training cohort, and its independent prognostic effect was further confirmed in the validation and entire cohorts. Based on previous literature, we collected 19 genes associated with cuproptosis. Afterward, 783 cuproptosis-related lncRNAs were obtained through coexpression. Cox model revealed and constructed eight cuproptosis-related lncRNAs prognostic marker (AL132800.1, AC090587.1, AC079160.1, AC011462.4, AL157888.1, GRHL3-AS1, SNHG16, and AC021148.2). Patients were divided into high- and low-risk groups based on the median risk score. The Kaplan-Meier survival curve revealed that the overall survival between the high- and low-risk groups was statistically significant. The receiver operating characteristic curve and principal component analysis demonstrated the accurate prognostic ability of the model. Univariate and multivariate Cox regression analysis showed that risk score was an independent prognostic factor. In addition, we used multivariate Cox regression to establish a nomogram of the predictive power of prognostic markers. The tumor mutation burden showed significant differences between the high- and low-risk groups. HNSC patients in the high-risk group responded better to immunotherapy than those in the low-risk group. We also found that risk scores were significantly associated with drug sensitivity in HNSC. In summary, our study identified eight cuprotosis-related lncRNAs signature of HNSC as the prognostic predictor, which may be promising biomarkers for predicting the benefit of HNSC immunotherapy as well as drug sensitivity.
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http://dx.doi.org/10.3389/fgene.2022.947551DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354258PMC
July 2022

The dynamic effects of maternal high-calorie diet on glycolipid metabolism and gut microbiota from weaning to adulthood in offspring mice.

Front Nutr 2022 28;9:941969. Epub 2022 Jul 28.

Department of Endocrinology, Peking University First Hospital, Beijing, China.

Dysbiosis of gut microbiota can contribute to the progression of diabetes and obesity. Previous studies have shown that maternal high-fat (HF) diet during the perinatal period can alter the microbiota and induce metabolic disorders at weaning. However, whether dysbiosis of gut microbiota and metabolism could be recovered by a normal diet after weaning and the dynamic changes of gut microbiota have not been fully studied. In this study, C57BL/6J female mice were fed with a normal chow (NC) or HF diet for 4 weeks preconception, during gestation, and until pup weaning. After weaning, male offspring were fed with an NC diet until 9 weeks of age. The microbiota of offspring at weaning and 9 weeks of age was collected for 16S rRNA gene amplicon sequencing. We found that dams fed with an HF diet showed glucose intolerance after lactation. Compared with the offspring from NC dams, the offspring from HF dams exhibited a higher body weight, hyperglycemia, glucose intolerance, hyperinsulinemia, hypercholesterolemia, and leptin resistance and lower adiponectin at weaning. Fecal analysis indicated altered microbiota composition between the offspring of the two groups. The decrease in favorable bacteria (such as ) and increase in unfavorable bacteria (such as and ) were strongly associated with a disturbance of glucose and lipid metabolism. After 6 weeks of normal diet, no difference in body weight, glucose, and lipid profiles was observed between the offspring of the two groups. However, the microbiota composition of offspring in the HF group was still different from that in the NC group, and microbiota diversity was lower in offspring of the HF group. The abundance of was lower in the offspring of the HF group. In conclusion, a maternal HF diet can induce metabolic homeostasis and gut microbiota disturbance in offspring at weaning. Gut microbiota dysbiosis can persist into adulthood in the offspring, which might have a role in the promotion of susceptibility to obesity and diabetes in the later life of the offspring.
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http://dx.doi.org/10.3389/fnut.2022.941969DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343994PMC
July 2022

Pyrroloquinoline quinone (PQQ) protects mitochondrial function of HEI-OC1 cells under premature senescence.

NPJ Aging 2022 Apr 19;8(1). Epub 2022 Apr 19.

Department of Otolaryngology and Head and Neck Surgery, Faculty of Medicine, University of Tokyo, Tokyo, Japan.

The aim of this study was to investigate the effects of pyrroloquinoline quinone (PQQ), an oxidoreductase cofactor, on the HO-induced premature senescence model in HEI-OC1 auditory cells and to elucidate its mechanism of action in vitro. Cells were treated with PQQ for 1 day before HO (100 μM) exposure. Mitochondrial respiratory capacity was damaged in this premature senescence model but was restored in cells pretreated with PQQ (0.1 nM or 1.0 nM). A decrease in mitochondrial potential, the promotion of mitochondrial fusion and the accelerated movement of mitochondria were all observed in PQQ-pretreated cells. The protein expression of sirtuin 1 (SIRT1) and peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α) were significantly decreased under HO exposure while they were increased with PQQ pretreatment, and PGC-1α acetylation was significantly decreased. In conclusion, PQQ has a protective effect on the premature senescence model of HEI-OC1 auditory cells and is associated with the SIRT1/PGC-1α signaling pathway, mitochondrial structure, and mitochondrial respiratory capacity.
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http://dx.doi.org/10.1038/s41514-022-00083-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9158787PMC
April 2022

Pharmacological Inhibition of STING/TBK1 Signaling Attenuates Myeloid Fibroblast Activation and Macrophage to Myofibroblast Transition in Renal Fibrosis.

Front Pharmacol 2022 18;13:940716. Epub 2022 Jul 18.

Department of Anesthesiology, Foshan Women and Children Hospital, Foshan, China.

Renal fibrosis is an important pathological biomarker of chronic kidney disease (CKD). Stimulator of interferon genes/TANK binding kinase 1 (STING/TBK1) axis has been identified as the main regulator of innate immune response and closely related to fibrotic disorder. However, the role of STING/TBK1 signaling pathway in kidney fibrosis is still unknown. In this study, we investigated the effect of pharmacological inhibition of STING/TBK1 signaling on renal fibrosis induced by folic acid (FA). In mice, TBK1 was significantly activated in interstitial cells of FA-injured kidneys, which was markedly inhibited by H-151 (a STING inhibitor) treatment. Specifically, pharmacological inhibition of STING impaired bone marrow-derived fibroblasts activation and macrophage to myofibroblast transition in folic acid nephropathy, leading to reduction of extracellular matrix proteins expression, myofibroblasts formation and development of renal fibrosis. Furthermore, pharmacological inhibition of TBK1 by GSK8612 reduced myeloid myofibroblasts accumulation and impeded macrophage to myofibroblast differentiation, resulting in less deposition of extracellular matrix protein and less severe fibrotic lesion in FA-injured kidneys. In cultured mouse bone marrow-derived monocytes, TGF-β1 activated STING/TBK1 signaling. This was abolished by STING or TBK1 inhibitor administration. In addition, GSK8612 treatment decreased levels of α-smooth muscle actin and extracellular matrix proteins and prevents bone marrow-derived macrophages to myofibroblasts transition . Collectively, our results revealed that STING/TBK1 signaling has a critical role in bone marrow-derived fibroblast activation, macrophages to myofibroblasts transition, and kidney fibrosis progression.
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http://dx.doi.org/10.3389/fphar.2022.940716DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340478PMC
July 2022

Effects of Glycolysis-Related Genes on Prognosis and the Tumor Microenvironment of Hepatocellular Carcinoma.

Front Pharmacol 2022 18;13:895608. Epub 2022 Jul 18.

Department of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics and National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China.

Hepatocellular carcinoma (HCC) is a common and deadly malignancy worldwide. Current treatment methods for hepatocellular carcinoma have many disadvantages; thus, it is urgent to improve the efficacy of these therapies. Glycolysis is critical in the occurrence and development of tumors. However, survival and prognosis biomarkers related to glycolysis in HCC patients remain to be fully identified. Glycolysis-related genes (GRGs) were downloaded from "The Molecular Signatures Database" (MSigDB), and the mRNA expression profiles and clinical information of HCC patients were obtained from TCGA. Consensus clustering was performed to classify the HCC patients into two subgroups. We used the least absolute shrinkage and selection operator (LASSO) regression analysis to construct the risk signature model. Kaplan-Meier (K-M) survival analysis was performed to evaluate the prognostic significance of the risk model, and the receiver operating characteristic (ROC) curve analysis was used to evaluate the prediction accuracy. The independent prediction ability of the risk model was validated by univariate and multivariate Cox regression analyses. The differences of immune infiltrates and relevant oncogenic signaling between different risk groups were compared. Finally, biological experiments were performed to explore the functions of screened genes. HCC patients were classified into two subgroups, according to the expression of prognostic-related GRGs. Almost all GRGs categorized in cluster 2 showed upregulated expressions, whereas GRGs in cluster 1 conferred survival advantages. GSEA identified a positive correlation between cluster 2 and the glycolysis process. Ten genes were selected for risk signature construction. Patients were assigned to high-risk and low-risk groups based on the median risk score, and K-M survival analysis indicated that the high-risk group had a shorter survival time. Additionally, the risk gene signature can partially affect immune infiltrates within the HCC microenvironment, and many oncogenic pathways were enriched in the high-risk group, including glycolysis, hypoxia, and DNA repair. Finally, knockdown of ME1 suppressed proliferation, migration, and invasion of hepatocellular carcinoma cells. In our study, we successfully constructed and verified a novel glycolysis-related risk signature for HCC prognosis prediction, which is meaningful for classifying HCC patients and offers potential targets for the treatment of hepatocellular carcinoma.
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http://dx.doi.org/10.3389/fphar.2022.895608DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340275PMC
July 2022

Why Do Chinese Employees Complain at the Workplace? An Exploratory Study Based on the Theory of Work Adjustment.

Front Psychol 2022 15;13:920041. Epub 2022 Jul 15.

Sun Wah International Business School, Liaoning University, Shenyang, China.

This study aims to investigate the causes of workplace complaints among Chinese employees and to develop a scale to measure them, drawing on the theory of work adjustment. We first obtained 49 items regarding employees' complaints following rigorous item generation and refinement procedures. Then, we conducted a survey with convenience sampling and obtained a sample of 268 employees. The exploratory factor analysis based on this sample generated a six-factor solution that explained 65.85% of the variance. The six factors include four person-environment (P-E) interactional factors, namely, dissatisfaction due to (a) interpersonal relationships; (b) management systems; (c) work conditions; and (d) authoritarian leadership; and two P-E misfit factors, namely, perceived misfit regarding (e) work content; and (f) job responsibilities. Furthermore, we obtained another sample of 349 employees through snowball sampling, on which we further validated the six-correlated-factor solution through confirmatory factor analysis. This study contributes to the literature by identifying causes of Chinese employees' complaints different from those attributed to their counterparts in Western cultures. This outcome particularly reveals that "dissatisfaction with interpersonal relationships" with colleagues was the leading cause of complaints among Chinese employees, rather than the "misfit between employees' needs and organizational rewards" revealed by Western culture-based studies. Both our findings and the scale we developed have practical implications for companies that employ Chinese employees.
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http://dx.doi.org/10.3389/fpsyg.2022.920041DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9335288PMC
July 2022

High-Altitude Drives the Convergent Evolution of Alpha Diversity and Indicator Microbiota in the Gut Microbiomes of Ungulates.

Front Microbiol 2022 7;13:953234. Epub 2022 Jul 7.

College of Life Sciences, Qufu Normal University, Qufu, China.

Convergent evolution is an important sector of evolutionary biology. High-altitude environments are one of the extreme environments for animals, especially in the Qinghai Tibet Plateau, driving the inquiry of whether, under broader phylogeny, high-altitude factors drive the convergent evolution of Artiodactyla and Perissodactyla gut microbiomes. Therefore, we profiled the gut microbiome of Artiodactyla and Perissodactyla at high and low altitudes using 16S rRNA gene sequencing. According to cluster analyses, the gut microbiome compositions of high-altitude Artiodactyla and Perissodactyla were not grouped together and were far from those of low-altitude Artiodactyla and Perissodactyla. The Wilcoxon's test in high-altitude ungulates showed significantly higher Sobs and Shannon indices than in low-altitude ungulates. At the phylum level, Firmicutes and Patescibacteria were significantly enriched in the gut microbiomes of high-altitude ungulates, which also displayed a higher Firmicutes/Bacteroidetes value than low-altitude ungulates. At the family level, Ruminococcaceae, Christensenellaceae, and Saccharimonadaceae were significantly enriched in the gut microbiomes of high-altitude ungulates. Our results also indicated that the OH and FH groups shared two significantly enriched genera, and . These findings indicated that a high altitude cannot surpass the order level to drive the convergent evolution of ungulate gut microbiome composition but can drive the convergent evolution of alpha diversity and indicator microbiota in the gut microbiome of ungulates. Overall, this study provides a novel perspective for understanding the adaptation of ungulates to high-altitude environments.
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http://dx.doi.org/10.3389/fmicb.2022.953234DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301279PMC
July 2022

A prospective study of total urinary deoxynivalenol in adolescents in Shanghai, China.

Chemosphere 2022 Jul 18:135727. Epub 2022 Jul 18.

CAS Key Laboratory of Nutrition, Metabolism and Food Safety, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, 200031, China. Electronic address:

There is growing interest in deoxynivalenol (DON) exposure during puberty because experimental evidence shows that DON-exposed to adolescents are more sensitive to DON and have limited detoxification ability. Nevertheless, there have been few surveys of DON exposure for adolescents in China. Furthermore, little is known about the effects of collection times on risk exposure. In the study, we estimated the risk of DON exposure for adolescents in Shanghai and explore the effects of collection time, areas, sex and BMI on intake estimates. A total of 315 adolescents aged 14-16 years, including 161 boys and 154 girls, were recruited. Urine samples were collected for three consecutive days and digested with β-glucuronidase and then purified using a DON-immunoaffinity column (IAC). Total deoxynivalenol levels were measured using liquid chromatography-tandem mass spectrometry (LC-MS/MS) in combination with a stable isotope dilution assay (SIDA). DON was detected in 945 morning urine samples taken from 315 individuals for three consecutive days. The mean estimated dietary intake of DON did not exceed the tolerable daily intake (TDI) of l μg/kg·bw/day, showing that exposure of adolescents in Shanghai is not of concern. However, 10-20% of probable daily intake (PDI) values exceed the TDI, indicating potential adverse effects. In addition, the DON concentration at the population level did not differ for urine samples collected at different times except for those of overweight adolescents. Therefore, assessment of exposure to DON by monitoring the morning urine of a healthy adolescent, except for overweight people, provides an appropriate estimate of exposure and related risk at the population level, but intake estimates for individuals are uncertain; these could be used to assess exposure of adolescents to DON rapidly and effectively for epidemiological investigations.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135727DOI Listing
July 2022

Efficacy and safety of a sequential treatment with clearing heat and eliminating phlegm and tonifying and activating blood circulation in treating acute ischemic stroke: study protocol for a randomized controlled trial.

J Tradit Chin Med 2022 Aug;42(4):604-610

The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou 510120, China.

Objective: To investigate the short-term efficacy and safety outcomes following a sequential treatment with clearing heat and eliminating phlegm (CHEP) formula and tonifying Qi and activating blood circulation (TQABC) formula in patients with acute ischemic stroke (AIS) within a 72 h time window.

Methods: In this randomized, multicenter, double-blinded, placebo-controlled trial, 500 participants will be randomly assigned in a ratio of 1∶1 to the CHEP+ TQABC group or control group. In addition to guideline-based standard medical care, participants in the treatment group will receive the CHEP formula for the first 5 consecutive days followed by the TQABC formula for another 10 consecutive days, while those in the control group will receive CHEP formula placebo and TQABC formula placebo consecutively. The primary outcome measure will be the comparison of the change in the National Institutes of Health Stroke Scale score from baseline to 15 days after randomization. The secondary outcome measures will include the scores on the modified Rankin Scale, Barthel Index, Patient-Reported Outcomes, TCM symptom pattern (Zheng-hou) evaluation Scale, and the incidence of in-hospital complications. Safety assessment will include the physical examination, laboratory detection, any adverse events or serious adverse events, and the proportion of any complications during hospitalization.

Discussion: The results of this study will provide objective and scientific data with which to assess the efficacy and safety of a sequential treatment based on "integrating disease and symptom pattern" for patients with AIS.
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http://dx.doi.org/10.19852/j.cnki.jtcm.20220617.003DOI Listing
August 2022

Functional network characteristics based on EEG of patients in acute ischemic stroke: A pilot study.

NeuroRehabilitation 2022 Jul 15. Epub 2022 Jul 15.

Department of Neurology, Dongzhimen Hospital, Beijing University of ChineseMedicine, Beijing, China.

Background: Ischemic stroke is a common type of stroke associated with reorganization of functional network of the brain.

Objective: This pilot study aimed to investigate the characteristics of functional brain networks based on EEG in patients with acute ischemic stroke.

Methods: Seven patients with ischemic stroke within 72 hours of onset and seven healthy controls were enrolled in the study. Dynamic EEG monitoring and clinical information were repeatedly collected within 72 hours (T1), on the 5th day (T2), and on the 7th day (T3) of stroke onset. A directed transfer function was employed to construct functional brain connection patterns. Graph theoretical analysis was performed to evaluate the characteristics of functional brain networks.

Results: First, we found that the brain networks of ischemic stroke patients were quite different from the healthy controls. The clustering coefficient (0.001 <  Threshold <  0.2) in Delta, Theta, and Alpha bands for the patients were significantly lower (P <  0.01) and the shortest path length in all bands (0.001 <  Threshold <  0.2) for the patients were significantly longer (P <  0.01). Moreover, the peaks of the shortest path length for the patients seemed to be higher in all bands with larger thresholds. Secondly, the brain networks for the patients showed a characterized time-variation pattern. The clustering coefficient (0.001 <  Threshold <  0.2) of T1 was higher than that of T2 in alpha band (P <  0.01). The shortest path length (0.001 <  Threshold <  0.2) of T3 was shorter than that of T2 (P <  0.01) in all bands, and the peak of T3 was numerically higher than that of T2 in all bands with narrower thresholds.

Conclusion: Functional brain networks in patients with acute ischemic stroke showed impaired global functional integration and decreased efficiency of information transmission compared with healthy subjects. The shortening of the shortest path length during the recovery indicates neural plasticity and reorganization.
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http://dx.doi.org/10.3233/NRE-220107DOI Listing
July 2022

Atractylenolide III Attenuates Angiogenesis in Gastric Precancerous Lesions Through the Downregulation of Delta-Like Ligand 4.

Front Pharmacol 2022 30;13:797805. Epub 2022 Jun 30.

TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Blocking and even reversing gastric precancerous lesions (GPL) is a key measure to lower the incidence of gastric cancer. Atractylenolide III (AT-III) is a mainly active component of the Atractylodes rhizome and has been widely used in tumor treatment. However, the effects of AT-III on GPL and its mechanisms have not been reported. H & E staining and AB-PAS staining were employed to evaluate the histopathology in the gastric mucosa. In parallel, CD34 immunostaining was performed for angiogenesis assessment, and transmission electron microscope for microvessel ultrastructural observation. Investigation for the possible mechanism and was conducted using immunohistochemistry, RT-qPCR and western blotting. In most GPL specimens, AT-III treatment reduced microvascular abnormalities and attenuated early angiogenesis, with the regression of most intestinal metaplasia and partial dysplasia. Meanwhile, the expression of VEGF-A and HIF-1α was enhanced in GPL samples of model rats, and their expressions were decreased in AT-III-treated GPL rats. Moreover, DLL4 mRNA and protein expression were higher in GPL rats than in control rats. DLL4 protein expression was significantly enhanced in human GPL tissues. In addition, AT-III treatment could diminish DLL4 mRNA level and protein expression in the MNNG-induced GPL rats. study showed that in AGS and HGC-27 cells, DLL4 mRNA level and protein expression were significantly decreased after AT-III treatment. However, AT-III had no significant regulatory effect on Notch1 and Notch4. AT-III treatment is beneficial in lessening gastric precancerous lesions and attenuating angiogenesis in rats, and that may be contributed by the decrease of angiogenesis-associated HIF-1α and VEGF-A, and downregulation of DLL4.
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http://dx.doi.org/10.3389/fphar.2022.797805DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9282052PMC
June 2022

Pretransplant Dialysis and Preemptive Transplant in Living Donor Kidney Recipients.

Kidney360 2022 06 18;3(6):1080-1088. Epub 2022 Apr 18.

Division of Nephrology, Department of Medicine, University of California San Francisco, San Francisco, California.

Background: The optimal timing of dialysis access placement in individuals with stage 5 CKD is challenging to estimate. Preemptive living donor kidney transplant (LDKT) is the gold-standard treatment for ESKD due to superior graft survival and mortality, but dialysis initiation is often required. Among LDKT recipients, we sought to determine which clinical characteristics were associated with preemptive transplant. Among non-preemptive LDKT recipients, we sought to determine what dialysis access was used, and their duration of use before receipt of living donor transplant.

Methods: We retrospectively extracted data on 569 LDKT recipients, >18 years old, who were transplanted between January 2014 and July 2019 at UCSF, including dialysis access type (arteriovenous fistula [AVF], arteriovenous graft [AVG], peritoneal dialysis catheter [PD], and venous catheter), duration of dialysis, and clinical characteristics.

Results: Preemptive LDKT recipients constituted 30% of our cohort and were older, more likely to be White, more likely to have ESKD from polycystic kidney disease, and less likely to have ESKD from type 2 diabetes. Of the non-preemptive patients, 26% used AVF, 0.5% used AVG, 32% used peritoneal catheter, 11% used venous catheter, and 31% used more than one access type. Median (IQR) time on dialysis for AVF/AVG use was 1.86 (0.85-3.32) years; for PD catheters, 1.12 (0.55-1.92) years; for venous catheters, 0.66 (0.23-1.69) years; and for multimodal access, 2.15 (1.37-3.72) years.

Conclusions: We characterized the dialysis access landscape in LDKT recipients. Venous catheter and PD were the most popular modality in the first quartile of dialysis, and patients using these modalities had shorter times on dialysis compared with those with an AVF. Venous catheter or PD can be considered a viable bridge therapy in patients with living donor availability given their shorter waitlist times. Earlier referral of patients with living donor prospects might further minimize dialysis need.
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http://dx.doi.org/10.34067/KID.0007652021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9255866PMC
June 2022

[11]-chaetoglobosins with cytotoxic activities from Pseudeurotium bakeri.

Bioorg Chem 2022 Oct 10;127:106011. Epub 2022 Jul 10.

School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, Wuhan 430030, People's Republic of China. Electronic address:

Fourteen new [11]-chaetoglobosins (1-14), along with two known congeners, cytochalasins X and Y (15 and 16), were isolated from the cultures of an endophytic fungus Pseudeurotium bakeri P1-1-1. Their structures incorporating absolute configurations were elucidated based on the comprehensive analyses of one- and two-dimensional NMR data, HRESIMS spectrometry, chemical methods, and single-crystal X-ray diffraction analysis (Cu Kα). All isolates were evaluated for their cytotoxic activities and chaetopseudeurin M (1) displayed significant cytotoxic effects against seven human cancer cell lines, with IC values ranging from 5.1 ± 0.9 to 10.8 ± 0.1 μM. Western blot experiments exhibited that compound 1 exerted its cytotoxic effect in MCF-7 cells by inducing G2/M cell cycle arrest and apoptosis via downregulating the expression of cyclin B1 and Cdk1, and activating Bcl-2/caspase-3/PARP pathway, respectively.
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http://dx.doi.org/10.1016/j.bioorg.2022.106011DOI Listing
October 2022

Modified Endoscopic Dacryocystorhinostomy: A Clubhead-Shaped Nasal Mucoperiosteal Flap Technique.

Laryngoscope 2022 Jul 15. Epub 2022 Jul 15.

Department of Otolaryngology-Head & Neck Surgery, Peking University First Hospital, Beijing, China.

A modified endoscopic dacryocystorhinostomy technique using a clubhead-shaped nasal flap combined with a posteriorly hinged lacrimal flap was designed for tension-free anastomosis with the surrounding mucoperiosteum. This technique showed satisfactory outcomes that facilitated the maintenance of the rhinostomy in eight cases with nasolacrimal duct obstruction. Laryngoscope, 2022.
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http://dx.doi.org/10.1002/lary.30265DOI Listing
July 2022

Tough Love: Impact of High-Performance Work System on Employee Innovation Behavior.

Front Psychol 2022 23;13:919993. Epub 2022 Jun 23.

School of Economics and Management, Shaanxi University of Science and Technology, Xi'an, China.

Based on the social exchange theory, this paper discusses the impact of high-performance work system (HPWS) on employee innovation behavior, constructs the mediating model of challenge stress and the moderated mediation model, and explores the influence mechanism of HPWS on employee innovation behavior under the management mode of combining strictness and love formed by "strictness" under the effect of challenge stress and "love" given by perceived organizational support. Through hierarchical regression analysis of 227 employees' survey data, the results show that HPWS positively influences employee innovation behavior. Challenge stress partially mediates the above relationship, and perceived organizational support positively moderates the mediating effect of challenge stress between HPWS and employee innovation behavior.
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http://dx.doi.org/10.3389/fpsyg.2022.919993DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9260179PMC
June 2022

Analysis of the safety and efficacy of tacrolimus combined with glucocorticoid in the treatment of lupus nephritis.

Pak J Med Sci 2022 May-Jun;38(5):1285-1291

Xiao-qiang Shi, Department of Urology, Affiliated Hospital of Hebei University, Baoding 071000, Hebei, P.R. China.

Objective: To evaluate the safety and efficacy of tacrolimus combined with glucocorticoids in the treatment of lupus nephritis.

Methods: A total of 80 patients with lupus nephritis were admitted to the Affiliated Hospital of Hebei University and the First Hospital of Baoding from February 2017 to January 2019 randomly divided into two groups: the experimental group and the control group, with 40 cases in each group. Patients in the experimental group were treated with tacrolimus combined with glucocorticoids, while patients in the control group were treated with cyclophosphamide combined with glucocorticoids for one year. Clinical efficacy and adverse drug reactions were evaluated for all patients after treatment. The changes of CRP, IL-6, 24h urinary protein, serum albumin, serum creatinine, urea nitrogen and other indicators after treatment, as well as the differences in the erythrocyte sedimentation rate (ESR), complement C3, C4, anti-dsDNA antibody positive rate and SLEDAI score and other indicators were also evaluated.

Results: The total efficacy of the experimental group was 92.5%, which was significantly better than the 75% of the control group (p=0.03); The incidence of adverse reactions was 20% in the experimental group and 42.5% in the control group, with a statistically significant difference (p=0.03). After treatment, the levels of CRP, IL-6 and other inflammatory factors in the experimental group were lower than those in the control group, with a statistical significance (p<0.05); The indicators of the experimental group such as 24h urine protein quantification, serum albumin, blood creatinine, and urea nitrogen were improved compared with the control group, with statistically highly significant differences (p<0.001). In addition, ESR, anti-DSDNA antibody positive rate and SLEDAI score were decreased compared with the control group, while complement C3 and C4 levels were significantly increased (p<0.05).

Conclusion: Tacrolimus combined with glucocorticoids is a safe and effective treatment regimen for patients with lupus nephritis, boasting a variety of benefits, such as significant efficacy and fewer adverse reactions. With such a regimen, the level of inflammatory factors can be significantly reduced, renal function indicators can be ameliorated, the ESR, complement C3, C4, anti-dsDNA antibody positive rate and SLEDAI score of the patients can be significantly improved.
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http://dx.doi.org/10.12669/pjms.38.5.5117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9247779PMC
August 2021

Effects of Continuous Fascia Iliaca Compartment Block on Early Quality of Recovery After Total Hip Arthroplasty in Elderly Patients: A Randomized Controlled Trial.

J Pain Res 2022 28;15:1837-1844. Epub 2022 Jun 28.

Department of Anesthesiology, Shenzhen Hospital of Southern Medical University, Shenzhen, 518110, People's Republic of China.

Purpose: In recent years, patient-centered postoperative quality of recovery has gained attention. This study aimed to assess the influence of ultrasound-guided continuous fascia iliaca compartment block (CFICB) on early quality of recovery in elderly patients after total hip arthroplasty (THA) using the QoR-15 score.

Patients And Methods: In this single-center, randomized, prospective study, 60 patients scheduled for unilateral THA were randomized to the CFICB or patient-controlled intravenous analgesia (PCIA) group. The primary outcome was the QoR-15 score. The secondary outcomes were pain score, number of patients requiring rescue analgesics, time of first postoperative ambulation, incidence of postoperative complications, Bromage score, and length of hospital stay.

Results: The QoR-15 score was significantly higher in the CFICB group than in the PCIA group at 24 h (P < 0.001) after surgery. However, the QoR-15 score was not significantly different at 48 h (P = 0.074) between the two groups. Pain scores at rest and during movement were lower in the CFICB group than in the PCIA group at 12, 24, and 48 h postoperatively (P < 0.05). There was no difference in the number of patients requiring rescue analgesics, time of first postoperative ambulation, incidence of postoperative complications apart from dizziness, or length of hospital stay between the two groups. In addition, Bromage score of 1 point was reported by four patients in the CFICB group at 24 h (P = 0.048) after THA.

Conclusion: In elderly patients following THA, CFICB improved the quality of recovery at 24 h and reduced pain scores compared with PCIA. The time of first postoperative ambulation and length of hospital stay were not significantly affected.
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http://dx.doi.org/10.2147/JPR.S368285DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9250328PMC
June 2022

Molecular Spectrum, Ethnic and Geographical Distribution of Thalassemia in the Southern Area of Hainan, China.

Front Pediatr 2022 10;10:894444. Epub 2022 Jun 10.

Department of Laboratory Medicine, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Background: Thalassemia is one of the most common genetic diseases in southern China. Accurate population frequency data regarding the occurrence and distribution of thalassemia are important for designing appropriate prevention strategies for thalassemia. This study aims to reveal the molecular spectrum, ethnic and geographical distribution of thalassemia in the southern area of Hainan Province, China.

Methods: A total of 9813 suspected carriers of thalassemia were screened for genetic analysis by using the PCR-reverse dot blot hybridization method targeting three known deletions of α-thalassemias (--, -α, and -α), three nondeletional mutations of α-thalassaemias (α, α, and α) and the 17 most common mutations of β-thalassaemias in the Chinese population.

Results: Approximately 6,924 subjects were genetically diagnosed as thalassemia carriers or patients, including 5812 cases of α-thalassemia (83.9%), 369 cases of β-thalassemia (5.3%), and 743 cases of α-composite β-thalassemia (10.7%). A total of 21 distinct genotypes were identified among the 5,812 α-thalassemia carriers, -α/αα, -α/αα, and -α/-α were the most common α-thalassemia genotypes. The most frequent β-thalassemia genotype was β/β, with a notable proportion of 69.6%, followed by the β /β, β/β, β/β, β/β, and β/β genotypes. In addition, 37 genotypes were detected among the 743 cases of both α- and β-thalassemia mutations. The α-thalassemia genotypes were most commonly found in the Li people, who accounted for 73.5% of α-thalassemia carriers. The β-thalassemia genotypes were most commonly identified in the Han people, who accounted for 59.4% of β-thalassemia carriers. Among the subjects carrying both α- and β-thalassemia variations, only three ethnic minorities were identified, including the Li, Han, and Miao people, accounting for 82.0, 17.4, and 0.7%, respectively.

Conclusions: Our study indicates that there is high genetic heterogeneity, geographical and ethnic differences in thalassemia in populations in the southern area of Hainan Province. These findings will be helpful in guiding genetic counseling and prenatal diagnosis of thalassemia in Hainan Province.
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http://dx.doi.org/10.3389/fped.2022.894444DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9245522PMC
June 2022

Cerebral Small Vessel Disease: Neuroimaging Features, Biochemical Markers, Influencing Factors, Pathological Mechanism and Treatment.

Front Neurol 2022 14;13:843953. Epub 2022 Jun 14.

Department of Neurology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China.

Cerebral small vessel disease (CSVD) is the most common chronic vascular disease involving the whole brain. Great progress has been made in clinical imaging, pathological mechanism, and treatment of CSVD, but many problems remain. Clarifying the current research dilemmas and future development direction of CSVD can provide new ideas for both basic and clinical research. In this review, the risk factors, biological markers, pathological mechanisms, and the treatment of CSVD will be systematically illustrated to provide the current research status of CSVD. The future development direction of CSVD will be elucidated by summarizing the research difficulties.
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http://dx.doi.org/10.3389/fneur.2022.843953DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9237477PMC
June 2022

Development, validation, and clinical application of a rapid UPLC-MS/MS method for detection of colchicine in human whole blood and urine.

Biomed Chromatogr 2022 Jun 29:e5437. Epub 2022 Jun 29.

Drug and Poison Testing Center, Tianjin Medical University General Hospital, Tianjin, China.

A rapid, simple, and economical method has been developed to determine colchicine in both human whole blood and urine using UPLC-MS/MS. Colchicine and isotope-labeled internal standard were extracted from the matrix by liquid-liquid extraction with saturated borax and ethyl acetate, and separated by a reversed-phase chromatography C18 column. Gradient elution was carried out using acetonitrile and water spiked with 0.01% formic acid. Multiple reaction monitoring was performed at positive ion mode. The quantitative transitions were m/z 400.27 → 310.28 for colchicine and m/z 406.16 → 313.18 for colchicine-D6. The method has good linearity in the range of 0.5-200 ng/mL for blood and 2-2000 ng/mL for urine. The sensitivity, accuracy, and matrix effect were all in line with the guidelines of Food and Drug Administration and European Medicines Agency. The extraction recovery was above 63.94%. The samples were stable under various storage conditions. Six deuterium-substituted isotopic internal standard was used to demonstrate a different mode of colchicine cleavage from the existing literature. This method has been successfully used in the diagnosis of patients with colchicine poisoning. Blood is recommended as the optimal sample compared with urine.
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http://dx.doi.org/10.1002/bmc.5437DOI Listing
June 2022

A Hemagglutinin Stem Vaccine Designed Rationally by AlphaFold2 Confers Broad Protection against Influenza B Infection.

Viruses 2022 06 14;14(6). Epub 2022 Jun 14.

State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Life Sciences, Xiamen University, Xiamen 361102, China.

Two lineages of influenza B viruses (IBV) co-circulating in human beings have been posing a significant public health burden worldwide. A substantial number of broadly neutralizing antibodies (bnAbs) have been identified targeting conserved epitopes on hemagglutinin (HA) stem domain, posing great interest for universal influenza vaccine development. Various strategies to design immunogens that selectively present these conserved epitopes are being explored. However, it has been a challenge to retain native conformation of the HA stem region, especially for soluble expression in prokaryotic systems. Here, using a structure prediction tool AlphaFold2, we rationally designed a stable stem antigen "B60-Stem-8071", an HA stem vaccine derived from B/Brisbane/60/2006 grafted with a CR8071 epitope as a linker. The B60-Stem-8071 exhibited better solubility and more stable expression in the system compared to the naïve HA stem antigen. Immunization with B60-Stem-8071 in mice generated cross-reactive antibodies and protected mice broadly against lethal challenge with Yamagata and Victoria lineages of influenza B virus. Notably, soluble expression of B60-stem-8071 in the system showed the potential to produce the influenza B vaccine in a low-cost way. This study represents a proof of concept for the rational design of HA stem antigen based on structure prediction and analysis.
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http://dx.doi.org/10.3390/v14061305DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9229588PMC
June 2022

Preceding Phenological Events Rather than Climate Drive the Variations in Fruiting Phenology in the Desert Shrub .

Plants (Basel) 2022 Jun 15;11(12). Epub 2022 Jun 15.

Institute of Desertification Studies, Chinese Academy of Forestry, Beijing 100091, China.

Fruit setting and ripening are crucial in the reproductive cycle of many desert plant species, but their response to precipitation changes is still unclear. To clarify the response patterns, a long-term in situ water addition experiment with five treatments, namely natural precipitation (control) plus an extra 25%, 50%, 75%, and 100% of the local mean annual precipitation (145 mm), was conducted in a temperate desert in northwestern China. A whole series of fruiting events including the onset, peak, and end of fruit setting and the onset, peak, and end of fruit ripening of a locally dominant shrub, , were observed from 2012 to 2018. The results show that (1) water addition treatments had no significant effects on all six fruiting events in almost all years, and the occurrence time of almost all fruiting events remained relatively stable compared with leaf phenology and flowering phenology after the water addition treatments; (2) the occurrence times of all fruiting events were not correlated to the amounts of water added in the treatments; (3) there are significant inter-annual variations in each fruiting event. However, neither temperature nor precipitation play key roles, but the preceding flowering events drive their inter-annual variation.
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http://dx.doi.org/10.3390/plants11121578DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9227467PMC
June 2022

Rare Variants in Novel Candidate Genes Associated With Nonsyndromic Patent Ductus Arteriosus Identified With Whole-Exome Sequencing.

Front Genet 2022 6;13:921925. Epub 2022 Jun 6.

Department of Pediatric Cardiology, Xin Hua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Patent ductus arteriosus (PDA) is one of the most common congenital heart defects causing pulmonary hypertension, infective endocarditis, and even death. The important role of genetics in determining spontaneous ductal closure has been well-established. However, as many of the identified variants are rare, thorough identification of the associated genetic factors is necessary to further explore the genetic etiology of PDA. We performed whole-exome sequencing (WES) on 39 isolated nonsyndromic PDA patients and 100 healthy controls. Rare variants and novel genes were identified through bioinformatic filtering strategies. The expression patterns of candidate genes were explored in human embryo heart samples. Eighteen rare damaging variants of six novel PDA-associated genes (, , , , , and ) were newly identified, which were highly expressed in human embryo hearts. WES is an efficient diagnostic tool for exploring the genetic pathogenesis of PDA. These findings contribute new insights into the molecular basis of PDA and may inform further studies on genetic risk factors for congenital heart defects.
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http://dx.doi.org/10.3389/fgene.2022.921925DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207465PMC
June 2022

A Novel Light Field Image Compression Method Using EPI Restoration Neural Network.

Biomed Res Int 2022 13;2022:8324438. Epub 2022 Jun 13.

School of Mechanical, Electrical & Information Engineering, Putian University, Putian, Fujian 351100, China.

Different from traditional images, light field images record not only spatial information but also angle information. Due to the large volume of light field data brings great difficulties to storage and compression, light field compression technology has attracted much attention. The epipolar plane image (EPI) contains a lot of low rank information, which is suitable for recovering the complete EPI from a part of EPI. In this paper, a light field image coding framework based on EPI restoration neural network has been proposed. Compared with previous algorithms, the proposed algorithm further takes advantage of the inherent similarity in light field images, and the proposed framework has higher performance and robustness. Experimental results show that the proposed method has superior performance compared to the state-of-the-art both in quantitatively and qualitatively.
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http://dx.doi.org/10.1155/2022/8324438DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9209000PMC
June 2022

Construction of m6A-Related lncRNA Prognostic Signature Model and Immunomodulatory Effect in Glioblastoma Multiforme.

Front Oncol 2022 2;12:920926. Epub 2022 Jun 2.

Department of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics, and National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China.

Background: Glioblastoma multiforme (GBM), the most prevalent and aggressive of primary malignant central nervous system tumors (grade IV), has a poor clinical prognosis. This study aimed to assess and predict the survival of GBM patients by establishing an m6A-related lncRNA signaling model and to validate its validity, accuracy and applicability.

Methods: RNA sequencing data and clinical data of GBM patients were obtained from TCGA data. First, m6A-associated lncRNAs were screened and lncRNAs associated with overall survival in GBM patients were obtained. Subsequently, the signal model was established using LASSO regression analysis, and its accuracy and validity are further verified. Finally, GO enrichment analysis was performed, and the influence of this signature on the immune regulation response and anticancer drug sensitivity of GBM patients was discussed.

Results: The signature constructed by four lncRNAs AC005229.3, SOX21-AS1, AL133523.1, and AC004847.1 is obtained. Furthermore, the signature proved to be effective and accurate in predicting and assessing the survival of GBM patients and could function independently of other clinical characteristics (Age, Gender and mutation). Finally, Immunosuppression-related factors, including APC co-inhibition, T-cell co-inhibition, CCR and Check-point, were found to be significantly up-regulated in GBM patients in the high-risk group. Some chemotherapeutic drugs (Doxorubicin and Methotrexate) and targeted drugs (AZD8055, BI.2536, GW843682X and Vorinostat) were shown to have higher IC50 values in patients in the high-risk group.

Conclusion: We constructed an m6A-associated lncRNA risk model to predict the prognosis of GBM patients and provide new ideas for the treatment of GBM. Further biological experiments can be conducted on this basis to validate the clinical value of the model.
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http://dx.doi.org/10.3389/fonc.2022.920926DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201336PMC
June 2022
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