Publications by authors named "Ying Gao"

1,194 Publications

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Comparative Study of COVD-19 Pandemic Progressions in 175 Regions in Australia, Canada, Italy, Japan, Spain, UK and USA Using a Novel Model That Considers Testing Capacity and Deficiency in Confirming Infected Cases.

IEEE J Biomed Health Inform 2021 Jun 15;PP. Epub 2021 Jun 15.

Not identified as being exposed or infected, the group of asymptomatic and presymptomatic patients has become the key source of infectious hosts for the COVID-19 pandemic, triggering the re-emergence or second waves of outbreaks. Acknowledging the impacts of movement of unidentified patients and the limited testing capacity on understanding the spread of the virus, an augmented Susceptible-Exposed-Infectious-Confirmed-Recovered (SEICR) model integrating intercity migration data and testing capacity is developed to probe into the number of unidentified COVID-19 infected patients. This model allows evaluation of the effectiveness of active interventions, and more accurate prediction of the pandemic progression in a country, region or city. A pseudo-coevolutionary algorithm is adopted in the model fitting to provide an effective estimation of high-dimensional unknown parameter sets using a limited amount of historical data. The model is applied to 175 regions in Australia, Canada, Italy, Japan, Spain, the UK and USA to estimate the number of unconfirmed cases using limited historical data. Results showed that the actual number of infected cases could be 4.309 times as many as the official confirmed number. By implementing mass COVID-19 testing, the number of infected cases could be reduced by about 50\%. Gearing up for mass testing would be essential for health authorities to acquire accurate and realistic information regarding the scope of the outbreak, which will help formulate timely public health responses.
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http://dx.doi.org/10.1109/JBHI.2021.3089577DOI Listing
June 2021

First-generation species-selective chemical probes for fluorescence imaging of human senescence-associated β-galactosidase.

Chem Sci 2020 Jun 17;11(28):7292-7301. Epub 2020 Jun 17.

State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology Shanghai 200237 China

Human senescence-associated β-galactosidase (SA-β-gal), the most widely used biomarker of aging, is a valuable tool for assessing the extent of cell 'healthy aging' and potentially predicting the health life span of an individual. Human SA-β-gal is an endogenous lysosomal enzyme expressed from , the catalytic domain of which is very different from that of β-gal, a bacterial enzyme encoded by . However, existing chemical probes for this marker still lack the ability to distinguish human SA-β-gal from β-gal of other species, such as bacterial β-gal, which can yield false positive signals. Here, we show a molecular design strategy to construct fluorescent probes with the above ability with the aid of structure-based steric hindrance adjustment catering to different enzyme pockets. The resulting probes normally work as traditional SA-β-gal probes, but they are unique in their powerful ability to distinguish human SA-β-gal from β-gal, thus achieving species-selective visualization of human SA-β-gal for the first time. NIR-emitting fluorescent probe as their representative further displays excellent species-selective recognition performance in biological systems, which has been herein verified by testing in senescent cells, in -transfected cells and in -β-gal-contaminated tissue sections of mice. Because of our probes, it was also discovered that SA-β-gal content in mice increased gradually with age and SA-β-gal accumulated most in the kidneys among the main organs of naturally aging mice, suggesting that the kidneys are the organs with the most severe aging during natural aging.
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http://dx.doi.org/10.1039/d0sc01234cDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8159415PMC
June 2020

PID: An integrative and comprehensive platform of plant intron.

Comput Biol Chem 2021 Jun 5;93:107528. Epub 2021 Jun 5.

Agricultural Big-Data Research Center and College of Plant Protection, Shandong Agricultural University, Daizong Road No. 61, Tai'an, Shandong, 271018, China. Electronic address:

Intron is a non-coding sequence of a broken gene and participates in important biological processes, such as transcription regulation, alternative splicing, and nuclear export. With the development of plant genomes, a comprehensive platform for intron analysis in plants must be established. Plant Intron Database (PID), a publicly available searchable database, was developed to efficiently store, query, analyze, and integrate intron resources in plants. The information of intron, exon, and gene can be searched by key words in PID. Users cannot only view intron length distribution pie chart and 5' and 3' splice site sequence feature maps in a statistical interface but can also browse intron information in a graphical visualization interface through JBrowse. ViroBlast for sequence homology searches, Intron detection and sequence interception tools were also provided. PID contains annotated genes from 118 sequenced plants, 24,782,048 introns, 30,843,049 exons, and 414 visual maps. This tool will greatly accelerate research on the distribution, length characteristics, and functions of introns in plants. PID is accessible at http://biodb.sdau.edu.cn/PID/index.php.
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http://dx.doi.org/10.1016/j.compbiolchem.2021.107528DOI Listing
June 2021

Rational Modification of Two-Dimensional Donor-Acceptor Covalent Organic Frameworks for Enhanced Visible Light Photocatalytic Activity.

ACS Appl Mater Interfaces 2021 Jun 7;13(23):27041-27048. Epub 2021 Jun 7.

Beijing Key Laboratory for Science and Application of Functional Molecular and Crystalline Materials, Department of Chemistry, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.

Covalent organic frameworks (COFs) are promising crystalline materials for photocatalytic solar- to hydrogen-energy conversion due to the tunable chemical structures and energy band gaps. Herein, we report a chemical modification strategy for improving the photocatalytic activity of COFs. A benzene-1,3,5-tricarbaldehyde (BT)- and benzothiadiazole derivative-based two-dimensional donor-acceptor (D-A) COF, denoted as BT-COF, were fabricated and further modified by using an alternative electron-donating unit, 2-hydroxybenzene-1,3,5-tricarbaldehyde (HBT), to the polycondensation reaction, yielding HBT-COF with an enhanced internal D-A effect and hydrophilicity. Interestingly, the photocatalytic H production rate of HBT-COF reaches 19.00 μmol h, which is 5 times higher than that of BT-COF (3.40 μmol h) under visible light irradiation. The increase in photocatalytic activity of HBT-COF is rationally attributed to finely tuned energy levels and improved wettability, which in turn leads to broadened visible light absorption, efficient photoinduced charge separation and transfer, and enhanced interactions between the COF catalyst and reaction substrates. The present results demonstrate that a subtle structural modification can significantly modulate the band structure and interfacial property, thus providing a feasible strategy for the optimization of COF-based photocatalytic systems.
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http://dx.doi.org/10.1021/acsami.1c04880DOI Listing
June 2021

GTPBP2 positively regulates the invasion, migration and proliferation of non-small cell lung cancer.

J Cancer 2021 5;12(13):3819-3826. Epub 2021 May 5.

Department of Pathology, The Fourth Hospital Affiliated to China Medical University, Shenyang, Liaoning. 110032 People's Republic of China.

Lung cancer is one of the most common malignant tumors in the world, and the mortality rate ranks first among various malignant tumors. GTP-binding proteins (guanosine 5'-triphosphate-binding proteins, GTPBPs) are a type of protein with signal transduction function, have GTP hydrolase activity, and play an important role in cell signal transmission, cytoskeletal regulation, protein synthesis and other activities. GTPBP2 is one of the members of the G protein superfamily. Research on GTPBP2 is currently focused on human genetics, and its research in tumors has not been reported. First, Western blot and quantitative real-time PCR were used to analyze the expression differences of 12 cases of GTPBP2 in human NSCLC fresh cancer tissues and adjacent tissues. Then we selected 112 cases of NSCLC cancer tissues and 65 adjacent tissues for immunohistochemistry experiments to analyze the relationships between the expression of GTPBP2 and clinical pathological parameters and prognosis, we found that GTPBP2 is highly expressed in NSCLC cancer tissues, and the high expression of GTPBP2 is related to pTNM stage and lymph node metastasis. In addition, after GTPBP2 knockdown, GTPBP2 can promote the proliferation and invasion of NSCLC cell lines by up-regulating RhoC and MMP-9, and up-regulate cyclinD1, CDK4 and c-myc, and down-regulate P27 to promote the invasion of NSCLC cell lines. In addition, GTPBP2 negatively regulates Axin to promote β-catenin expression, thereby activating Wnt/β-catenin signaling, and promoting the occurrence of NSCLC.
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http://dx.doi.org/10.7150/jca.48340DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176249PMC
May 2021

Physical activity accumulation along the intensity spectrum differs between children and adults.

Eur J Appl Physiol 2021 Jun 5. Epub 2021 Jun 5.

Faculty of Sport and Health Sciences, Neuromuscular Research Center, University of Jyväskylä, Jyväskylä, Finland.

Purpose: Detailed exploration of physical activity accumulation with fine grading along the intensity spectrum has indicated the potential pragmatic utility of such an approach. However, it is currently unclear what sorts of accumulation patterns along particular intensity bands are found in the children and adult populations. Therefore, we conducted a comparison of activity accumulation in specific intensity bands between four distinct populations: children, adults with sedentary lifestyles, habitual joggers, habitual marathon runners.

Methods: Free-living waist-worn accelerometry records from 28 children aged 7 to 11, and 61 adults aged 25 to 35 were analysed. Activity intensity was evaluated in 5 s non-overlapping epochs as mean amplitude deviation (MAD) and normalised to acceleration intensities corresponding to walking at 3 metabolic equivalents of a task (METs). Adult data were normalised to 0.091 g MAD based on literature, and data from children to 0.170 g MAD based on laboratory experimentation. The normalised epoch values were divided into 100 intensity gradations.

Results: Children accumulated more activity in 0.74 to 1.58 normalised acceleration intensities (all p < 0.005) compared to adults. Adult joggers/runners accumulated more activity in normalised acceleration intensities from 7.1 to 11.1 compared to the other groups (p < 0.008).

Conclusion: The primary bulk of children's free-living activities are of relatively low intensity not likely to provoke cardiometabolic improvement. These sorts of explorations could be used in informing intervention development aiming at optimising healthy development. Evidence is mounting to justify randomised controlled trials based on intervention targets identified based on exploring the intensity spectrum.
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http://dx.doi.org/10.1007/s00421-021-04731-3DOI Listing
June 2021

Chinese Herbal Medicine Xingnaojing Injection for Acute Ischemic Stroke: An Overview of Systematic Reviews and Meta-Analyses.

Front Pharmacol 2021 18;12:659408. Epub 2021 May 18.

Centre for Evidence Based Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China.

Xingnaojing injection (XNJ) is the only Chinese herbal injection approved for both intracerebral hemorrhage and ischemic stroke (IS) first-aid on ambulances in China; many systematic reviews (SRs) and meta-analyses (MAs) of XNJ on stroke have been published. The purpose of this research was to evaluate and summarize the current evidence on XNJ for IS. A comprehensive search was conducted for SRs and MAs of XNJ on IS in seven databases up to January 1, 2021. Two authors independently identified SRs and MAs, extracted data, assessed the quality of the included SRs and MAs using the Assessment of Multiple Systematic Reviews 2 (AMSTAR 2), and assessed quality of evidence using the Grading of Recommendations, Assessment, Development, and Evaluation (GRADE). A total of 10 SRs met the inclusion criteria. The quality of included SRs using AMSTAR 2 was critically low as the critical items were poorly reported. Only 10% of SRs reported 50% of the 16 items, while the remaining 90% SRs reported just less than half of the items on AMSTAR 2. For GRADE, 7 (35%) of outcomes had low-quality evidence, 10 (50%) with very low, and 3 (15%) with moderate quality evidence. Very low to low quality of evidence indicated XNJ plus conventional therapy (CT) alleviated the neurological deficits of acute IS. Moderate-quality evidence showed XNJ plus CT reduced mortality when compared to Danshen injection plus CT, and very low-quality evidence showed XNJ plus CT could not improve the degree of coma, while low-quality evidence indicated the opposite. Mild adverse events in the CT group were less than those in the XNJ plus CT group, and there were no serious adverse events, but there was no statistical difference between the two groups. The included 10 SRs indicated that XNJ was used for acute IS, but only four SRs (40%) reported the course of disease. XNJ appears to be effective and safe for acute IS in improving the neurological deficits, but the evidence is not robust enough. However, whether administering XNJ immediately after or within 24 h of IS is best remains unknown due to the lack of data. Well-designed large-scale randomized controlled trials with measurable outcomes are required in future studies.
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http://dx.doi.org/10.3389/fphar.2021.659408DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167030PMC
May 2021

Trichodermin Induces G0/G1 Cell Cycle Arrest by Inhibiting c-Myc in Ovarian Cancer Cells and Tumor Xenograft-Bearing Mice.

Int J Mol Sci 2021 May 9;22(9). Epub 2021 May 9.

College of Health, Science, Technology and Mathematics, Alderson Broaddus University, Philippi, WV 26416, USA.

Ovarian cancer is a fatal gynecological cancer because of a lack of early diagnosis, which often relapses as chemoresistant. Trichodermin, a trichothecene first isolated from , is an inhibitor of eukaryotic protein synthesis. However, whether trichodermin is able to suppress ovarian cancer or not was unclear. In this study, trichodermin (0.5 µM or greater) significantly decreased the proliferation of two ovarian cancer cell lines A2780/CP70 and OVCAR-3. Normal ovarian IOSE 346 cells were much less susceptible to trichodermin than the cancer cell lines. Trichodermin predominantly inhibited ovarian cancer cells by inducing G0/G1 cell cycle arrest rather than apoptosis. Trichodermin decreased the expression of cyclin D1, CDK4, CDK2, retinoblastoma protein, Cdc25A, and c-Myc but showed little effect on the expression of p21, p27, or p16. c-Myc was a key target of trichodermin. Trichodermin regulated the expression of Cdc25A and its downstream proteins via c-Myc. Overexpression of c-Myc attenuated trichodermin's anti-ovarian cancer activity. In addition, trichodermin decelerated tumor growth in BALB/c nude mice, proving its effectiveness in vivo. These findings suggested that trichodermin has the potential to contribute to the treatment of ovarian cancer.
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http://dx.doi.org/10.3390/ijms22095022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8126000PMC
May 2021

Urinary C-peptide/creatinine ratio: A useful biomarker of insulin resistance and refined classification of type 2 diabetes mellitus.

J Diabetes 2021 May 29. Epub 2021 May 29.

Departments of Endocrinology and Metabolism, Peking University People's Hospital, Peking University Diabetes Center, Beijing, China.

Background: The urinary C-peptide/creatinine ratio (UCPCR) is low in patients with type 1 diabetes mellitus, but it has not been well characterized in patients with type 2 diabetes mellitus (T2DM). We aimed to measure the UCPCRs in patients with T2DM and explore the relationships among UCPCR, insulin resistance (IR), and chronic vascular complications of diabetes.

Methods: A cross-sectional study was performed of 1299 Chinese hospitalized patients with T2DM. Binary logistic regression was used to evaluate the relationships between the chronic vascular complications of diabetes and UCPCR. K-means analysis was used to allocate participants to subgroups with five to six variables (age at diagnosis, body mass index [BMI], glycosylated hemoglobin, homoeostasis model assessment 2-estimated beta-cell function (HOMA2-B), and HOMA2-insulin resistance (HOMA2-IR), with or without UCPCR).

Results: UCPCR positively correlated with HOMA2-IR (r = 0.448, P < .001). After adjustment for sex, age, duration of diabetes, and other cardiovascular risk factors, UCPCR was positively associated with diabetic kidney disease (DKD) (odds ratio [OR] = 1.198, 95% CI 1.019-1.408, P = .029) and coronary heart disease (CHD) (OR = 1.312, 95% CI 1.079-1.594, P = .006). When UCPCR was added, cluster analysis using the six variables identified five subgroups of T2DM, characterized by differing age at diagnosis, BMI, beta-cell function, IR, and prevalence of vascular complications.

Conclusions: UCPCR is positively associated with IR, DKD, and CHD and represents a promising biomarker that could refine the classification of T2DM.
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http://dx.doi.org/10.1111/1753-0407.13203DOI Listing
May 2021

Synergistic effect of carbon starvation and exogenous redox mediators on corrosion of X70 pipeline steel induced by Desulfovibrio singaporenus.

Sci Total Environ 2021 Sep 23;788:147573. Epub 2021 May 23.

Key Laboratory of Marine Environmental Corrosionand Bio-Fouling, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Open Studio for Marine Corrosion and Protection, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China.

In microbiologically influenced corrosion (MIC) induced by sulfate-reducing bacteria (SRB), the electrons released from iron were transferred via extracellular electron transfer (EET) to the inner cells. Electron mediators and carbon starvation have also been found to promote steel corrosion. This study aimed to investigate the synergistic effects of electron mediators and carbon starvation on MIC and their effect on biofilm catalytic activity. The results demonstrated that the weight losses of X70 steel were 0.68 and 1.03 mg/cm in 100% and 10% carbon source (CS) SRB solution, respectively. The addition of riboflavin and cytochrome c increased the corrosion rate by 1.76 and 1.87 times, respectively, in the 100% CS SRB medium compared to the medium without exogenous redox mediators. For the 10% CS SRB medium, the corrosion rate increased by 1.40 and 1.89 times, respectively, when riboflavin and cytochrome c were added. The addition of riboflavin and cytochrome c also enhanced the biocatalytic activity of the SRB biofilm in both the 100% and 10% CS SRB media.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147573DOI Listing
September 2021

Evaluating the effect of fluoxetine on mesophilic anaerobic dark biohydrogen fermentation of excess sludge.

Bioresour Technol 2021 May 21;336:125320. Epub 2021 May 21.

School of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao 266033, China.

Recently, the influence behavior of new pollutants in the environment has been widely concerned. However, the effect of antidepressants widely detected in excess sludge (ES) on biohydrogen production from anaerobic dark fermentation has never been explored. To fill this gap, fluoxetine (FLX), a typical antidepressant, was selected to evaluate its effect on ES mesophilic anaerobic dark biohydrogen fermentation. The results showed that FLX reduced biohydrogen production even at low content (0.1 mg/Kg). The biohydrogen yield was only 12.8 mL/g in the 1.8 mg/Kg (based on total suspended solids) FLX group, decreased by about 34.7%, compared with the control group (without FLX). Further mechanism investigation implied that high levels (more than 0.6 mg/Kg) of FLX reduced every step associated with the biohydrogen production. FLX reduced the concentration of ammonia nitrogen and phosphate in fermentation broth. FLX also had a significant negative effect on enzyme activity in ES dark fermentation.
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http://dx.doi.org/10.1016/j.biortech.2021.125320DOI Listing
May 2021

Nucleation and growth mechanisms of an electrodeposited Ni-Se-Cu coating on nickel foam.

J Colloid Interface Sci 2021 May 5;600:492-502. Epub 2021 May 5.

Powder Metallurgy Research Institute, Central South University, Changsha 410083, China.

Electrocatalysts for water splitting have been widely explored among recent years. In this study, nickel-selenium-copper (Ni-Se-Cu) coating was synthesized on nickel foam through potentiostatic electrodeposition. The electrochemical kinetics and nucleation mechanisms of the deposition were investigated, and the diffusion coefficient D from different deposition potentials and temperatures was calculated. Results reveal that the electrodeposition of Ni-Se-Cu follows an instantaneous nucleation and diffusion-controlled three-dimensional (3D) growth mechanism. Deposition potential and bath temperature slightly effect the nucleation mechanism of electrodeposition. The apparent activation energy E of the hydrogen evolution reaction (HER) in 1.0 M KOH electrolyte of Ni-Se-Cu is 21.1 kJ·mol, which is lower than that of Ni-Se (37.7 kJ·mol). The majority phase formed by nickel and selenium is NiSe, and a Ni(Cu) solid solution forms after the incorporation of Cu atoms into a Ni lattice.
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http://dx.doi.org/10.1016/j.jcis.2021.05.002DOI Listing
May 2021

Ultra-High Dose Rate FLASH Irradiation Induced Radio-Resistance of Normal Fibroblast Cells Can Be Enhanced by Hypoxia and Mitochondrial Dysfunction Resulting From Loss of Cytochrome C.

Front Cell Dev Biol 2021 30;9:672929. Epub 2021 Apr 30.

State Key Laboratory of Nuclear Physics and Technology, School of Physics and CAPT, Peking University, Beijing, China.

Ultra-high dose rate FLASH irradiation (FLASH-IR) has got extensive attention since it may provide better protection on normal tissues while maintain tumor killing effect compared with conventional dose rate irradiation. The FLASH-IR induced protection effect on normal tissues is exhibited as radio-resistance of the irradiated normal cells, and is suggested to be related to oxygen depletion. However, the detailed cell death profile and pathways are still unclear. Presently normal mouse embryonic fibroblast cells were FLASH irradiated (∼10 Gy/s) at the dose of ∼10-40 Gy in hypoxic and normoxic condition, with ultra-fast laser-generated particles. The early apoptosis, late apoptosis and necrosis of cells were detected and analyzed at 6, 12, and 24 h post FLASH-IR. The results showed that FLASH-IR induced significant early apoptosis, late apoptosis and necrosis in normal fibroblast cells, and the apoptosis level increased with time, in either hypoxic or normoxic conditions. In addition, the proportion of early apoptosis, late apoptosis and necrosis were significantly lower in hypoxia than that of normoxia, indicating that radio-resistance of normal fibroblast cells under FLASH-IR can be enhanced by hypoxia. To further investigate the apoptosis related profile and potential pathways, mitochondria dysfunction cells resulting from loss of cytochrome c (cyt c) were also irradiated. The results showed that compared with irradiated normal cells (cyt c), the late apoptosis and necrosis but not early apoptosis proportions of irradiated cyt c cells were significant decreased in both hypoxia and normoxia, indicating mitochondrial dysfunction increased radio-resistance of FLASH irradiated cells. Taken together, to our limited knowledge, this is the first report shedding light on the death profile and pathway of normal and cyt c cells under FLASH-IR in hypoxic and normoxic circumstances, which might help us improve the understanding of the FLASH-IR induced protection effect in normal cells, and thus might potentially help to optimize the future clinical FLASH treatment.
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http://dx.doi.org/10.3389/fcell.2021.672929DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121317PMC
April 2021

Association of Cancer Stem Cell Radio-Resistance Under Ultra-High Dose Rate FLASH Irradiation With Lysosome-Mediated Autophagy.

Front Cell Dev Biol 2021 29;9:672693. Epub 2021 Apr 29.

State Key Laboratory of Nuclear Physics and Technology, School of Physics, CAPT, Peking University, Beijing, China.

Cancer stem cell (CSC) is thought to be the major cause of radio-resistance and relapse post radiotherapy (RT). Recently ultra-high dose rate "FLASH-RT" evokes great interest for its decreasing normal tissue damages while maintaining tumor responses compared with conventional dose rate RT. However, the killing effect and mechanism of FLASH irradiation (FLASH-IR) on CSC and normal cancer cell are still unclear. Presently the radiation induced death profile of CSC and normal cancer cell were studied. Cells were irradiated with FLASH-IR (∼10 Gy/s) at the dose of 6-9 Gy via laser-accelerated nanosecond particles. Then the ratio of apoptosis, pyroptosis and necrosis were determined. The results showed that FLASH-IR can induce apoptosis, pyroptosis and necrosis in both CSC and normal cancer cell with different ratios. And CSC was more resistant to radiation than normal cancer cell under FLASH-IR. Further experiments tracing lysosome and autophagy showed that CSCs had higher levels of lysosome and autophagy. Taken together, our results suggested that the radio-resistance of CSC may associate with the increase of lysosome-mediated autophagy, and the decrease of apoptosis, necrosis and pyroptosis. To our limited knowledge, this is the first report shedding light on the killing effects and death pathways of CSC and normal cancer cell under FLASH-IR. By clarifying the death pathways of CSC and normal cancer cell under FLASH-IR, it may help us improve the understanding of the radio-resistance of CSC and thus help to optimize the future clinical FLASH treatment plan.
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http://dx.doi.org/10.3389/fcell.2021.672693DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116574PMC
April 2021

No obvious association exists between mean platelet volume and hypertension subtypes.

Biomark Med 2021 Jun 14;15(8):577-584. Epub 2021 May 14.

Tianjin Key Laboratory of Lung Cancer Metastasis & Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin, 300052, PR China.

To determine the association between mean platelet volume (MPV) and hypertension subtypes. 44,281 Chinese individuals were enrolled in this cross-sectional study. The mean blood pressure decreased with increasing MPV in females (p = 0.001) and increased MPV seemed to be a potential protective factor for isolated diastolic hypertension in models 1 and 2. The OR (CI) was 0.878 (0.789-0.976) for model 1 and 0.880 (0.789-0.981) for model 2 in males and 0.646 (0.495-0.841) for model 1 and 0.657 (0.503-0.858) for model 2 in females, when MPV was analyzed as a categorical variable. The OR (CI) was 0.947 (0.911-0.985) for Model 1 and 0.947 (0.910-0.985) for Model 2 in males, and 0.886 (0.807-0.973) for Model 1 and 0.892 (0.813-0.978) for Model 2 in females when MPV was analyzed as a continuous variable. However, the statistical difference of OR disappeared when we added blood-related covariates in Model 3. No obvious association exists between MPV and hypertension subtypes. Other blood parameters might have a greater impact on hypertension subtypes.
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http://dx.doi.org/10.2217/bmm-2020-0305DOI Listing
June 2021

The Effects of CSF Neurogranin and ε4 on Cognition and Neuropathology in Mild Cognitive Impairment and Alzheimer's Disease.

Front Aging Neurosci 2021 27;13:667899. Epub 2021 Apr 27.

Cerebrospinal fluid (CSF) measurements of neurogranin (Ng) have emerged as a promising biomarker for cognitive decline in mild cognitive impairment (MCI) and Alzheimer's disease (AD). The apolipoprotein E ε4 ( ε4) allele is by far the most consistent genetic risk factor for AD. However, it is not known whether the pathophysiological roles of Ng in MCI or AD are related to ε4. We stratified 250 participants from the Alzheimer's Disease Neuroimaging Initiative (ADNI) database into cognitively normal (CN) ε4 negative (CN ε4-), CN ε4 positive (CN ε4+), MCI ε4 negative (MCI ε4-), MCI ε4 positive (MCI ε4+), AD ε4 negative (AD ε4-), and AD ε4 positive (AD ε4+). CSF Ng levels were significantly increased in ε4 carriers compared to ε4 non-carriers with MCI. In addition, CSF Ng identified MCI ε4+ versus CN ε4-, but not MCI ε4- versus CN ε4-. Similarly, CSF Ng negatively correlated with Mini-Mental State Examination (MMSE) scores at baseline in the MCI ε4+ group. Our findings support the use of CSF Ng as a biomarker of synaptic pathology for AD. We propose that the roles of CSF Ng in the pathophysiology of MCI may be related to ε4.
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http://dx.doi.org/10.3389/fnagi.2021.667899DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8110906PMC
April 2021

[Screening of key genes and pathways of ischemic stroke and prediction of traditional Chinese medicines based on bioinformatics].

Zhongguo Zhong Yao Za Zhi 2021 Apr;46(7):1803-1812

Department of Neurology, Dongzhimen Hospital, Beijing University of Chinese Medicine Beijing 100700, China Institute for Brain Disorders, Beijing University of Chinese Medicine Bejing 100700, China.

The aim of this paper was to explore the key genes and pathogenesis of ischemic stroke(IS) by bioinformatics, and predict the potential traditional Chinese medicines for IS. Based on the gene-chip raw data set of GSE22255 from National Center of Biotechnology Information(NCBI), the article enrolled in 20 patients with ischemic stroke and 20 sex-and age-matched controls, and differentially expressed genes(DEGs) were screened based on R language software. The DAVID tool and R language software were used to perform gene ontology(GO) biological process enrichment analysis and Kyoto encyclopedia of genes and gnomes(KEGG) pathway enrichment analysis. The DEGs were imported into STRING to construct a protein-protein interaction network, and the Molecular Complexity Module(MCODE) plug-in of Cytoscape software was used to visualize and analyze the key functional modules. Moreover, the core genes and the medical ontology information retrieval platform(Coremine Medical) were mapped to each other to screen the traditional Chinese medicines and construct drug-active ingredient-target network. Compared with healthy controls, 14 DEGs were obtained, of which 12 genes were up-regulated and 2 genes were down-regulated. DEGs were mainly involved in immune response, inflammatory process, signal transduction, and cell proliferation regulation. The interleukin-17(IL-17), nuclear factor kappaB(NF-κB), tumor necrosis factor(TNF), nucleotide binding oligomerization domain(NOD)-like receptor and other signaling pathways were involved in KEGG pathway enrichment analysis. The key modules of the DEGs-encoding protein interaction network mainly focused on 7 genes of TNF, JUN, recombinant immediate early response 3(IER3), recombinant early growth response protein 1(EGR1), prostaglandin-endoperoxide synthase 2(PTGS2), C-X-C motif chemokine ligand 8(CXCL8) and C-X-C motif chemokine ligand 2(CXCL2), which were involved in biological processes widely such as neuroinflammation and immunity. TNF and JUN were the key nodes in this module, which might become potential biological markers for diagnosis and prognosis evaluation of IS. The potential traditional Chinese medicines for the treatment of IS includes Salviae Miltiorrhizae Radix et Rhizoma, Croci Stigma, Scutellariae Radix, and Cannabis Fructus. The occurrence of stroke was the result of multiple factors. Dysregulation of genes and pathways related to immune regulation and inflammation may be the key link for the development of IS. This study provided research direction and theoretical basis for further exploring the mechanism of action of traditional Chinese medicine in the treatment of IS and searching for potential drug targets.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20210218.401DOI Listing
April 2021

Glycosylation of Anti-Thyroglobulin IgG1 and IgG4 Subclasses in Thyroid Diseases.

Eur Thyroid J 2021 Apr 27;10(2):114-124. Epub 2020 Aug 27.

Department of Endocrinology, Peking University First Hospital, Beijing, China.

Objective: Thyroglobulin antibodies (TgAb), principally comprising immunoglobulin G (IgG), are frequently found in healthy individuals. Previously, we showed that the glycosylation levels of TgAb IgG differed across various thyroid diseases, suggesting an important role of glycosylation on antibodies in the pathogenesis of thyroid diseases. Since IgG1 and IgG4 are the primary TgAb IgG subclasses, this study aimed to investigate the glycosylation of TgAb IgG1 and IgG4 subclasses in thyroid diseases.

Methods: TgAb IgG was purified by affinity chromatography from the serum of patients with Hashimoto's thyroiditis (HT) ( = 16), Graves' disease (GD) ( = 8), papillary thyroid carcinoma (PTC) ( = 6), and PTC with histological lymphocytic thyroiditis (PTC-T) ( = 9) as well as healthy donors ( = 10). TgAb IgG1 and IgG4 concentrations were determined by enzyme-linked immunosorbent assay, and a lectin microassay was used to assess TgAb IgG1 and IgG4 glycosylation.

Results: Significantly elevated mannose, sialic acid, and galactose levels on TgAb IgG1 were found in HT and PTC patients compared to GD patients and healthy controls (all < 0.05). The mannose, sialic acid, and core fucose levels on TgAb IgG1 in PTC-T patients were higher than in healthy controls (all < 0.05). Additionally, TgAb IgG1 from PTC-T patients exhibited lower sialylation than that from patients with PTC and higher fucosylation than that from patients with HT (both < 0.05). However, TgAb IgG4 glycosylation did not differ among the five groups ( < 0.05).

Conclusion: Our study describes different distributions of TgAb IgG1 glycosylation in various thyroid diseases. The aberrantly increased glycosylation levels of TgAb IgG1 observed in HT, PTC, and PTC-T might be indicative of immune disorders and participate in the pathogenesis of these diseases.
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http://dx.doi.org/10.1159/000507699DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8077608PMC
April 2021

The Traditional Uses, Phytochemistry, Pharmacology, Toxicology, and Clinical Uses of (Franch.) T.N.Ho and S.W.Liu, an Ethnomedicine in Southwest China.

Front Pharmacol 2021 26;12:658628. Epub 2021 Apr 26.

The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang, China.

(Franch.) T.N.Ho and S.W.Liu (MR) belongs to Gentianales and it is often called Hong-hua-long-dan in Chinese. Traditionally, it has been used to cure acute icteric hepatitis, sore throat, dysentery, acute gastritis, carbuncle, and furuncle based on traditional Chinese medicine (TCM) concepts. This review manages to provide a critical and comprehensive analysis on the traditional uses, phytochemistry, pharmacology, toxicology, and clinical uses of MR and to evaluate the therapeutic potential of this plant. Relevant data mainly literatures on MR were selected from available database. All the papers reviewed provided evidence that the source herbs were reliably identified. The heat-clearing and removing the phlegm, and purging fire and removing toxicity of MR contribute to its dispelling jaundice, and clearing lung heat and cough. The compounds isolated from this plant include iridoids and secoiridoids, phenolic acids, ketones, triterpenoids, flavonoids, benzophenone glycosides, and others. Mangiferin (MAF) is a characteristic substance from this plant. The pharmacological studies show that some extracts and compounds from MR exhibit anti-inflammatory, antinociceptive, antibacterial, hepatoprotective, cardioprotective, and other effects which are associated with the traditional uses of this plant. The toxicological studies suggest that MAF is less toxic in mice and dogs. Nowadays, Chinese patent drugs such as Feilike Jiaonang and Kangfuling Jiaonang containing MR have been used to cure cough, asthma, chronic bronchitis, dysmenorrhea, and appendagitis. Although the current studies provide related research information of MR, it is still necessary to systemically evaluate the chemistry, pharmacology, toxicity, and safety of the extracts or compounds from this plant before clinical trials in the future. In addition, except for lung infection-related diseases, analgesia, anti-tumor, and hypertriglycemia may be new and prior therapeutic scopes of this ethnomedicine in the future.
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http://dx.doi.org/10.3389/fphar.2021.658628DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107381PMC
April 2021

Engineering Broad-Spectrum Resistance to Bacterial Blight by CRISPR/Cas9-Mediated Precise Homology Directed Repair in Rice.

Mol Plant 2021 May 7. Epub 2021 May 7.

National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China. Electronic address:

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http://dx.doi.org/10.1016/j.molp.2021.05.012DOI Listing
May 2021

Efficacy and Safety of (Xueshuantong) in Patients With Acute Ischemic Stroke (EXPECT) Trial: Rationale and Design.

Front Pharmacol 2021 22;12:648921. Epub 2021 Apr 22.

Institute for Brain Disorders, Beijing University of Chinese Medicine, Beijing, China.

Although revascularization treatment is recommended as the first-line therapy for patients with non-minor acute ischemic stroke (AIS), it only benefits a minority of patients. Previous studies have reported the positive effects of (PNS) (Xueshuantong lyophilized powder) on AIS, however, there have been no rigorous trials. This study aims to assess the efficacy and safety of PNS therapy for patients with AIS. The Evaluation of Xueshuantong in Patients with acutE ischemiC sTroke (EXPECT) trial is a multicenter, randomized, placebo-controlled, double-blind study aiming to enroll 480 patients in China. Eligible patients with AIS within 72 h of symptom onset will randomly receive either PNS or PNS placebo for 10 days and subsequently be followed up to 90 days. The primary outcome will be a change in the National Institute of Health Stroke Scale (NIHSS) score from baseline to 10 post-randomization days. The secondary outcomes include early neurological improvement (proportion of patients with NIHSS score 0-1), and Patient-Reported Outcomes Scale for Stroke score at 10 post-randomization days, the proportion of patients with life independence (modified Rankin Scale score of 0-1), the proportion of patients with a favorable outcome (Barthel Index ≥90), and Stroke-Specific Quality of Life score at 90 days. Adverse events or clinically significant changes in vital signs and laboratory parameters, regardless of the severity, will be recorded during the trial to assess the safety of PNS. To our knowledge, this study is the first double-blind trial to assess the efficacy and safety of PNS in patients with AIS. Findings of the EXPECT trial will be valuable in improving evidence regarding the clinical application of PNS therapy in patients with AIS ineligible for revascularization treatment in the reperfusion era.
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http://dx.doi.org/10.3389/fphar.2021.648921DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8101545PMC
April 2021

Anlotinib exerts anti-cancer efficiency on lung cancer stem cells in vitro and in vivo through reducing NF-κB activity.

J Cell Mol Med 2021 Jun 6;25(12):5547-5559. Epub 2021 May 6.

Lung Cancer Center, State Key Laboratory of Biotherapy, West China Hospital of Sichuan University, Chengdu, China.

Anlotinib is a multi-target tyrosine kinase inhibitor. Previous studies confirmed that anlotinib exerts anti-cancer efficiency. However, the functional roles of anlotinib on cancer stem cells (CSCs) are yet to be elucidated. In this study, lung CSCs were isolated and identified in vitro, and mouse xenografts were established in vivo. MTT assays, tumour sphere formation assays, TdT-mediated dUTP nick-end labelling (TUNEL) staining, Annexin V-FITC/PI staining, immunofluorescence analysis and Western blot were performed to investigate the anti-cancer effects of anlotinib on lung CSCs. The results showed that anlotinib inhibits the growth of lung CSCs in vitro and in vivo. In addition, anlotinib induced apoptosis of these cells along with down-regulated expression level of Bcl-2 whereas up-regulated Bax and cleaved caspase-3 expression. It also sensitized lung CSCs to the cytotoxicity of cisplatin and paclitaxel; the tumour sphere formation and expression levels of multiple stemness-associated markers, such as ALDH1 and CD133, were also decreased. Furthermore, the underlying mechanism indicated that anlotinib reduces the phosphorylated levels of NF-κB p65 and IκB-α in lung CSCs. Taken together, these findings suggested that anlotinib exerts potent anti-cancer effects against lung CSCs through apoptotic induction and stemness phenotypic attenuation. The mechanism could be associated with the suppression of NF-κB activity.
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http://dx.doi.org/10.1111/jcmm.16564DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184695PMC
June 2021

Neck circumference and waist circumference associated with cardiovascular events in type 2 diabetes (Beijing Community Diabetes Study 23).

Sci Rep 2021 May 4;11(1):9491. Epub 2021 May 4.

Diabetes Trials Unit, Radcliffe Department of Medicine, University of Oxford, Oxford, UK.

Obesity increases the risk of developing cardiovascular disease and other metabolic diseases. We intended to compare three different anthropometric indicators of obesity, in predicting the incidence of cardiovascular events in Chinese type 2 diabetes. Beijing Community Diabetes Study was a prospective multi-center study conducted in Beijing community health centers. Type 2 diabetes patients from fourteen community health centers were enrolled at baseline. The primary endpoint was cardiovascular events. The upper quartile of neck circumference (NC) was set as greater NC. A total of 3299 diabetes patients were enrolled. In which, 941 (28.52%) had cardiovascular disease at baseline. Logistic analysis showed that central obesity (waist circumference (WC) above 90 cm in men and 85 cm in women) and greater NC were all related to baseline cardiovascular disease (adjusted OR = 1.49, and 1.55). After 10-year follow-up, 340 (10.31%) had cardiovascular events. Compared with patients without cardiovascular events, those having cardiovascular events had higher BMI, larger WC and NC. Cox regression analysis showed that greater WC and NC were all associated with the occurrence of cardiovascular events (adjusted HR = 1.41, and 1.38). A higher NC and WC might increase the risk of cardiovascular events by about 40% in type 2 diabetes patients in Beijing communities.
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http://dx.doi.org/10.1038/s41598-021-88927-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097072PMC
May 2021

The Responses to Long-Term Water Addition of Soil Bacterial, Archaeal, and Fungal Communities in A Desert Ecosystem.

Microorganisms 2021 Apr 30;9(5). Epub 2021 Apr 30.

Institute of Desertification Studies, Chinese Academy of Forestry, Beijing 100091, China.

The response of microbial communities to continual and prolonged water exposure provides useful insight when facing global climate changes that cause increased and uneven precipitation and extreme rainfall events. In this study, we investigated an in situ manipulative experiment with four levels of water exposure (ambient precipitation +0%, +25%, +50%, and +100% of local annual mean precipitation) in a desert ecosystem of China. After 9 years of water addition, Illumina sequencing was used to analyze taxonomic compositions of the soil bacterial, archaeal, and fungal communities. The results showed significant increases in microbial biomass carbon (MBC) at higher amended precipitation levels, with the highest values reported at 100% precipitation. Furthermore, an increase in the bacterial species richness was observed along the water addition gradient. In addition, the relative abundance of several bacterial phyla, such as Proteobacteria significantly increased, whereas that of some drought-tolerant taxa, including Actinobacteria, Firmicutes, and Bacteroidetes, decreased. In addition, the phyla Planctomycetes and Nitrospirae, associated with nitrification, positively responded to increased precipitation. Archaeal diversity significantly reduced under 100% treatment, with changes in the relative abundance of Thaumarchaeota and Euryarchaeota being the main contributors to shifts in the archaeal community. The fungal community composition was stable in response to water addition. Results from the Mantel test and structural equation models suggested that bacterial and archaeal communities reacted contrastingly to water addition. Bacterial community composition was directly affected by changing soil moisture and temperature, while archaeal community composition was indirectly affected by changing nitrogen availability. These findings highlight the importance of soil moisture and nitrogen in driving microbial responses to long-term precipitation changes in the desert ecosystem.
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http://dx.doi.org/10.3390/microorganisms9050981DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147197PMC
April 2021

Emerging role of interleukin-13 in cardiovascular diseases: A ray of hope.

J Cell Mol Med 2021 Jun 4;25(12):5351-5357. Epub 2021 May 4.

Department of Cardiology, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Despite the great progress made in the treatment for cardiovascular diseases (CVDs), the morbidity and mortality of CVDs remains high due to the lack of effective treatment strategy. Inflammation is a central pathophysiological feature of the heart in response to both acute and chronic injury, while the molecular basis and underlying mechanisms remains obscure. Interleukin (IL)-13, a pro-inflammatory cytokine, has been known as a critical mediator in allergy and asthma. Recent studies appraise the role of IL-13 in CVDs, revealing that IL-13 is not only involved in more obvious cardiac inflammatory diseases such as myocarditis but also relevant to acute or chronic CVDs of other origins, such as myocardial infarction and heart failure. The goal of this review is to summarize the advancement in our knowledge of the regulations and functions of IL-13 in CVDs and to discuss the possible mechanisms of IL-13 involved in CVDs. We highlight that IL-13 may be a promising target for immunotherapy in CVDs.
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http://dx.doi.org/10.1111/jcmm.16566DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184673PMC
June 2021

Genetic analysis of the "head top shape" quality trait of Chinese cabbage and its association with rosette leaf variation.

Hortic Res 2021 May 1;8(1):106. Epub 2021 May 1.

State Key Laboratory of North China Crop Improvement and Regulation, Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, College of Horticulture, Hebei Agricultural University, 071000, Baoding, China.

The agricultural and consumer quality of Chinese cabbage is determined by its shape. The shape is defined by the folding of the heading leaves, which defines the head top shape (HTS). The overlapping HTS, in which the heading leaves curve inward and overlap at the top, is the shape preferred by consumers. To understand the genetic regulation of HTS, we generated a large segregating F population from a cross between pak choi and Chinese cabbage, with phenotypes ranging from nonheading to heading with either outward curving or inward curving overlapping heading leaves. HTS was correlated with plant height, outer/rosette leaf length, and petiole length. A high-density genetic map was constructed. Quantitative trait locus (QTL) analysis resulted in the identification of 22 QTLs for leafy head-related traits, which included five HTS QTLs. Bulked segregant analysis (BSA) was used to confirm HTS QTLs and identify candidate genes based on informative single-nucleotide polymorphisms. Interestingly, the HTS QTLs colocalized with QTLs for plant height, outer/rosette leaf, and petiole length, consistent with the observed phenotypic correlations. Combined QTL analysis and BSA laid a foundation for molecular marker-assisted breeding of Chinese cabbage HTS and directions for further research on the genetic regulation of this trait.
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http://dx.doi.org/10.1038/s41438-021-00541-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087666PMC
May 2021

Efficacy of statins on renal function in patients with chronic kidney disease: a systematic review and meta-analysis.

Ren Fail 2021 Dec;43(1):718-728

Health Management Center, Tianjin Medical University General Hospital, Tianjin, P.R. China.

Background: Studies have shown that the use of statins could significantly improve lipid profiles; however, it remains controversial whether the use of statins could improve renal function in patients with chronic kidney disease (CKD). Therefore, we conducted a meta-analysis of randomized controlled trials (RCTs) to evaluate the effects of statins on renal function in patients with CKD.

Methods: We systematically searched PubMed, EMBASE, and the Cochrane Library databases for eligible RCTs from inception to October 2020. Pooled effect estimates were assigned as weighted mean differences (WMDs) with 95% confidence intervals (CIs) using the random-effects model.

Results: We selected 33 RCTs that recruited 37,391 patients with CKD patients. The summary results suggested that statin use significantly reduced urinary albumin (WMD: -2.04; 95%CI: -3.53 to -0.56;  = .007) and protein (WMD: -0.58; 95%CI: -0.95 to -0.21;  = .002) excretions and increased creatinine clearance (WMD: 0.86; 95%CI: 0.32-1.41;  = .002). However, there were no significant differences between statin and control groups in terms of changes in estimated glomerular filtration rate (WMD: 0.38; 95%CI: -0.04 to 0.79;  = .075), and serum creatinine levels (WMD: -0.07; 95%CI: -0.25, 0.12;  = .475).

Conclusions: We found that statin use in patients with CKD may slow CKD progression by lowering urinary albumin and protein excretions or increasing creatinine clearance. Further large-scale RCTs should be conducted to evaluate the long-term effects of statins on renal outcomes. CKD: chronic kidney disease; RCT: randomized controlled trials; WMD: weighted mean differences; CI: confidence intervals; ACEI: angiotensin-converting enzyme inhibitors; eGFR: estimated glomerular filtration rate.
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http://dx.doi.org/10.1080/0886022X.2021.1915799DOI Listing
December 2021

Corrigendum: Combination of Tanshinone IIA and Cisplatin Inhibits Esophageal Cancer by Downregulating NF-κB/COX-2/VEGF Pathway.

Front Oncol 2021 6;11:670798. Epub 2021 Apr 6.

Department of Traditional Chinese Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

[This corrects the article DOI: 10.3389/fonc.2020.01756/full.].
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http://dx.doi.org/10.3389/fonc.2021.670798DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8057108PMC
April 2021

Prognostic value of NT-proBNP in patients with chronic coronary syndrome and normal left ventricular systolic function according to glucose status: a prospective cohort study.

Cardiovasc Diabetol 2021 04 22;20(1):84. Epub 2021 Apr 22.

State Key Laboratory of Cardiovascular Disease, FuWai Hospital, National Center for Cardiovascular Diseases, National Clinical Research Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 167 BeiLiShi Road, XiCheng District, 100037, Beijing, China.

Background: The prognostic value of N-terminal pro-brain natriuretic peptide (NT-proBNP) in patients with coronary artery disease (CAD) with different glucose status has not been established. This study sought to evaluate the significance of NT-proBNP in predicting major adverse cardiovascular events (MACEs) in patients with chronic coronary syndrome (CCS) and normal left-ventricular systolic function (LVSF) according to different glucose status, especially in those with abnormal glucose metabolism.

Methods: A total of 8062 patients with CCS and normal LVSF were consecutively enrolled in this prospective study. Baseline plasma NT-proBNP levels were measured. The follow-up data of all patients were collected. Kaplan-Meier and Cox regression analyses were used to assess the risk of MACEs according to NT-proBNP tertiles stratified by glucose status.

Results: Over an average follow-up of 59.13 ± 18.23 months, 569 patients (7.1 %) suffered from MACEs, including cardiovascular death, non-fatal myocardial infarction, and non-fatal stroke. Kaplan-Meier analysis showed that high NT-proBNP levels had a significant association with MACEs in subjects with prediabetes mellitus (pre-DM) or DM, but not in patients with normoglycemia. Multivariate Cox regression analysis revealed that NT-proBNP remained an independent predictor of MACEs in patients with pre-DM [hazard ratio (HR): 2.56, 95% confidence interval (CI): 1.34-4.91] or DM (HR: 2.34, 95% CI: 1.32-4.16). Moreover, adding NT-proBNP to the original Cox model including traditional risk factors significantly increased the C-statistic by 0.035 in pre-DM and DM, respectively.

Conclusions: The present study indicated that NT-proBNP could well predict worse outcomes in dysglycemic patients with CCS and normal LVSF, suggesting that NT-proBNP may help with risk stratification in this population.
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http://dx.doi.org/10.1186/s12933-021-01271-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063320PMC
April 2021

Health care reform and stroke prognosis in low-income Chinese populations from 1992 to 2018.

J Glob Health 2021 Apr 17;11:08002. Epub 2021 Apr 17.

Department of Neurology, Tianjin Medical University General Hospital, Tianjin, China.

Background: To assess the impact of the health care reform on stroke prognoses among low-income Chinese residents.

Methods: Stroke events and all-cause deaths were registered during 1992-2018 in Tianjin, China. Trends in stroke management and prognoses were compared during the study periods1992-2008 and 2009-2018.

Results: A total of 1462 patients were diagnosed with first-ever stroke during the study periods. For patients aged ≥45 years, the rates of neuroimaging-based diagnoses and hospitalization were greater in 2009-2018 than in 1992-2008, regardless of patient sex or stroke type. Overall, the one-year case fatality rate was significantly lower in 2009-2018 than in the earlier period; the case fatality rate for women aged ≥65 years decreased by 30.0%. Between both periods, the stroke recurrence rate increased 1.9-fold, including a 2.5-fold increase in men (all  < 0.05). During the 2009-2018 period, the one-year case fatality rate was higher among elderly male patients not using medical insurance than among those using it (32.8% vs 20.7%;  = 0.050). After 2009, a significant decline in the recurrence rate ( = 0.001) and a significant increase in the hospitalization rate ( = 0.004) were observed in the interrupted time-series analysis.

Conclusions: These findings suggest that the implementation of universal medical insurance for residents in urban and rural China played a major role in improving the prognoses of low-income, rural, first-ever stroke patients, especially for elderly (≥65 years old) residents. However, elderly male patients not using medical insurance benefits had a high case fatality rate. Thus, restructuring of the government medical insurance policy to facilitate its use by low-income, rural residents is crucial for reducing the stroke burden in China.
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http://dx.doi.org/10.7189/jogh.11.08002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8053405PMC
April 2021