Publications by authors named "Ying Fu"

514 Publications

The PdeK-PdeR two-component system promotes unipolar localization of FimX and pilus extension in pv. .

Sci Signal 2021 Sep 14;14(700):eabi9589. Epub 2021 Sep 14.

Department of Plant Pathology and MOA Key Laboratory of Pest Monitoring and Green Management, and Joint International Research Laboratory of Crop Molecular Breeding, Ministry of Education, China Agricultural University, Beijing 100193, China.

[Figure: see text].
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http://dx.doi.org/10.1126/scisignal.abi9589DOI Listing
September 2021

Photoactivated Osmium Arene Anticancer Complexes.

Inorg Chem 2021 Sep 9. Epub 2021 Sep 9.

Department of Chemistry, University of Warwick, Coventry CV4 7AL, U.K.

Half-sandwich Os-arene complexes exhibit promising anticancer activity, but their photochemistry has hardly been explored. To exploit the photocytotoxicity and photochemistry of Os-arenes, -chelated complexes [Os(η--cymene)(Curc)Cl] (, Curc = curcumin) and [Os(η-biphenyl)(Curc)Cl] (), and -chelated complexes [Os(η-biphenyl)(dpq)I]PF (, dpq = pyrazino[2,3-][1,10]phenanthroline) and [Os(η-biphenyl)(bpy)I]PF (, bpy = 2,2'-bipyridine), have been investigated. The Os-arene curcumin complexes showed remarkable photocytotoxicity toward a range of cancer cell lines (blue light IC: 2.6-5.8 μM, photocytotoxicity index PI = 23-34), especially toward cisplatin-resistant cancer cells, but were nontoxic to normal cells. They localized mainly in mitochondria in the dark but translocated to the nucleus upon photoirradiation, generating DNA and mitochondrial damage, which might contribute toward overcoming cisplatin resistance. Mitochondrial damage, apoptosis, ROS generation, DNA damage, angiogenesis inhibition, and colony formation were observed when A549 lung cancer cells were treated with . The photochemistry of these Os-arene complexes was investigated by a combination of NMR, HPLC-MS, high energy resolution fluorescence detected (HERFD), X-ray adsorption near edge structure (XANES) spectroscopy, total fluorescence yield (TFY) XANES spectra, and theoretical computation. Selective photodissociation of the arene ligand and oxidation of Os(II) to Os(III) occurred under blue light or UVA excitation. This new approach to the design of novel Os-arene complexes as phototherapeutic agents suggests that the novel curcumin complex , in particular, is a potential candidate for further development as a photosensitizer for anticancer photoactivated chemotherapy (PACT).
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http://dx.doi.org/10.1021/acs.inorgchem.1c00241DOI Listing
September 2021

USPIO-SWI Shows Fingolimod Enhanced Alteplase Action on Angiographic Reperfusion in eMCAO Rats.

J Magn Reson Imaging 2021 Sep 4. Epub 2021 Sep 4.

Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, Xi'an, China.

Background: Noninvasive evaluation of the status of cerebral arteriole perfusion remains a practical challenge in murine stroke models, because conventional magnetic resonance imaging (MRI) is no longer capable of capturing these very small vessels.

Purpose: To investigate the feasibility of ultrasmall superparamagnetic iron oxide particles (USPIO)-based susceptibility weighted imaging (SWI)-MRI (USPIO-SWI) and T2* map-MRI (USPIO-T2* map) for monitoring angiographic perfusion in stroke rats.

Study Type: A preclinical randomized controlled trial.

Animal Model: Normal rats (N = 9), embolic middle cerebral artery occlusion (eMCAO) rats (N = 66).

Field Strength/sequence: 7 T; T2* map (multigradient echo), SWI (3D gradient echo).

Assessment: Experiment 1: To develop a method for angiographic reperfusion evaluation with USPIO-SWI. Normal rats were used to optimize the USPIO dosage (5.6, 16.8, and 56 mg/kg ferumoxytol) as well as scan time points for cerebral arterioles. Contrast-to-noise ratio (CNR) was measured. Stroke rats were further used and the number of visual cortical vessels were counted. Experiment 2: To examine whether fingolimod (lymphocytes inhibitor) enhances the action of tissue plasminogen activator (tPA) in eMCAO rats on cerebral angiographic reperfusion.

Statistical Tests: Mann-Whitney test and two way-ANOVA were used. P < 0.05 was considered statistically significant.

Results: CNR values of cerebral cortical penetrating arteries in normal rats were significantly increased to 4.4 ± 0.5 (5.6 mg/kg), 6.1 ± 0.5 (16.8 mg/kg), and 3.4 ± 0.9 (56 mg/kg) after USPIO injection. The number of visual cortical vessels on USPIO-SWI images in ischemic regions was significantly less than in control regions (5 ± 2 vs. 56 ± 20) of eMCAO rats. Compared with eMCAO rats who received tPA only, eMCAO rats who received the combination of fingolimod and tPA exhibited significantly higher proportion of complete angiographic reperfusion (69% vs. 17%).

Data Conclusion: This study supports the feasibility of angiographic perfusion evaluation with USPIO-SWI in stroke rats.

Level Of Evidence: 2 TECHNICAL EFFICACY STAGE: 1.
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http://dx.doi.org/10.1002/jmri.27914DOI Listing
September 2021

HOMEOBOX PROTEIN 24 Mediates the Conversion of Indole-3-butyric acid to Indole-3-acetic acid to Promote Root Hair Elongation.

New Phytol 2021 Sep 4. Epub 2021 Sep 4.

State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.

Indole-3-acetic acid (IAA) is a predominant form of active auxin in plants. In addition to de novo biosynthesis and release from its conjugate forms, IAA can be converted from its precursor indole-3-butyric acid (IBA). The IBA-derived IAA may help drive root hair elongation in Arabidopsis thaliana seedlings, but how the IBA-to-IAA conversion is regulated and affects IAA function requires further investigation. In this study, HOMEOBOX PROTEIN 24 (HB24), a transcription factor in the zinc finger-homeodomain family (ZF-HD family) of proteins, was identified. With loss of HB24 function, defective growth occurred in root hairs. INDOLE-3-BUTYRIC ACID RESPONSE 1 (IBR1), which encodes an enzyme involved in the IBA-to-IAA conversion, was identified as a direct target of HB24 for the control of root hair elongation. The exogenous IAA or auxin analog 1-naphthalene acetic acid (NAA) both rescued the root hair growth phenotype of hb24 mutants, but IBA did not, suggesting a role for HB24 in the IBA-to-IAA conversion. Thus, HB24 participates in root hair elongation by up-regulating the expression of IBR1 and subsequently promoting the IBA-to-IAA conversion. Moreover, IAA also elevated the expression of HB24, suggesting a feedback loop is involved in IBA-to-IAA conversion-mediated root hair elongation.
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http://dx.doi.org/10.1111/nph.17719DOI Listing
September 2021

Specific Secondary Bile Acids Control Chicken Necrotic Enteritis.

Pathogens 2021 Aug 17;10(8). Epub 2021 Aug 17.

Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA.

Necrotic enteritis (NE), mainly induced by the pathogens of and coccidia, causes huge economic losses with limited intervention options in the poultry industry. This study investigated the role of specific bile acids on NE development. Day-old broiler chicks were assigned to six groups: noninfected, NE, and NE with four bile diets of 0.32% chicken bile, 0.15% commercial ox bile, 0.15% lithocholic acid (LCA), or 0.15% deoxycholic acid (DCA). The birds were infected with at day 18 and at day 23 and 24. The infected birds developed clinical NE signs. The NE birds suffered severe ileitis with villus blunting, crypt hyperplasia, epithelial line disintegration, and massive immune cell infiltration, while DCA and LCA prevented the ileitis histopathology. NE induced severe body weight gain (BWG) loss, while only DCA prevented NE-induced BWG loss. Notably, DCA reduced the NE-induced inflammatory response and the colonization and invasion of compared to NE birds. Consistently, NE reduced the total bile acids in the ileal digesta, while dietary DCA and commercial bile restored it. Together, this study showed that DCA and LCA reduced NE histopathology, suggesting that secondary bile acids, but not total bile acid levels, play an essential role in controlling the enteritis.
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http://dx.doi.org/10.3390/pathogens10081041DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8427939PMC
August 2021

Anastatin Derivatives Alleviate Myocardial Ischemia-Reperfusion Injury via Antioxidative Properties.

Molecules 2021 Aug 6;26(16). Epub 2021 Aug 6.

China International Science and Technology Cooperation Base of Food Nutrition/Safety and Medicinal Chemistry, College of Bioengineering, Tianjin University of Science and Technology, Tianjin 300457, China.

(±)-Anastatins A and B are flavonoids isolated from . In a previous study, twenty-four di- and tri-substituted novel derivatives of anastatins were designed and their preliminary antioxidant activities were evaluated. In the present study, the protective effect of myocardial ischemia-reperfusion (I/R) and the systematic antioxidant capacity of 24 derivatives were further studied. Compound was the most potent among all the compounds studied, which increased the survival of H9c2 cells to 80.82%. The antioxidant capability of compound was evaluated in ferric reducing antioxidant power, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging, and 2,2-diphenyl-1-picrylhydrazyl assays. It was observed that compound significantly reduced infarcted areas and improved histopathological and electrocardiogram changes in rats with myocardial I/R injury. Moreover, compound decreased the leakage rates of serum lactate dehydrogenase, creatine kinase, and malonyldialdehyde from rat myocardial tissues and increased the level of glutathione and superoxide dismutase activities following myocardial I/R injury in rats. Taken together, we concluded that compound had potent cardioprotective effects against myocardial I/R injury both in vitro and in vivo owing to its extensive antioxidant activities.
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http://dx.doi.org/10.3390/molecules26164779DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8399290PMC
August 2021

Identification and Development of KASP Markers for Novel Mutant Alleles Associated With Elevated Oleic Acid in .

Front Plant Sci 2021 26;12:715633. Epub 2021 Jul 26.

Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.

The fatty acid desaturase genes are the main contributors to oleic acid content, and different alleles can result in different oleic acid contents in rapeseed oil. Hence, identification of allelic variation in is an extremely desirable breeding goal. By performing QTL mapping using 190 F lines genotyped by genome-wide single nucleotide polymorphism (SNP) markers assayed by the 60 K Infinium BeadChip Array, four quantitative trait loci (QTL) for C18:1 content were mapped on chromosomes A01, A05, A09 and C05 over 3 years in a population segregating for oleic acid content. Two genes on A05 and C05 were anchored within the QTL intervals, explaining 45-52 and 15-44% of the observed variation for C18:1 content. Sequence polymorphisms between the corresponding coding regions of the parental lines found two single-nucleotide polymorphisms (SNPs) in and , respectively, which led to the amino acid changes (C421T and G1073E) in the corresponding proteins. The mutation sites of and alleles were located within the second H-box and near the third H-box motif of the protein, respectively, and were found to be novel mutant alleles. Lines resulting from the combination of these two alleles contained up to 88% oleic acid in their seed oil, compared with 63% in wild-type controls. Two competitive allele-specific PCR (KASP) markers based on these two mutation sites were successfully developed and validated in segregating F populations. These markers will facilitate breeding for ultra-high seed oleic acid content in oilseed rape.
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http://dx.doi.org/10.3389/fpls.2021.715633DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8350730PMC
July 2021

Physics-based Noise Modeling for Extreme Low-light Photography.

IEEE Trans Pattern Anal Mach Intell 2021 Aug 10;PP. Epub 2021 Aug 10.

Enhancing the visibility in extreme low-light environments is a challenging task. Under nearly lightless condition, existing image denoising methods could easily break down due to significantly low SNR. In this paper, we systematically study the noise statistics in the imaging pipeline of CMOS photosensors, and formulate a comprehensive noise model that can accurately characterize the real noise structures. Our novel model considers the noise sources caused by digital camera electronics which are largely overlooked by existing methods yet have significant influence on raw measurement in the dark. It provides a way to decouple the intricate noise structure into different statistical distributions with physical interpretations. Moreover, our noise model can be used to synthesize realistic training data for learning-based low-light denoising algorithms. In this regard, although promising results have been shown recently with deep convolutional neural networks, the success heavily depends on abundant noisy-clean image pairs for training, which are tremendously difficult to obtain in practice. Extensive experiments on multiple low-light denoising datasets -- including a newly collected one in this work covering various devices -- show that a deep neural network trained with our proposed noise formation model can reach surprisingly-high accuracy. The results are on par with or sometimes even outperform training with paired real data.
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http://dx.doi.org/10.1109/TPAMI.2021.3103114DOI Listing
August 2021

Time-Resolved FDTD and Experimental FTIR Study of Gold Micropatch Arrays for Wavelength-Selective Mid-Infrared Optical Coupling.

Sensors (Basel) 2021 Jul 31;21(15). Epub 2021 Jul 31.

RISE Research Institutes of Sweden AB, Box 1070, SE-164 25 Kista, Sweden.

Infrared radiation reflection and transmission of a single layer of gold micropatch two-dimensional arrays, of patch length ∼1.0 μm and width ∼0.2 μm, have been carefully studied by a finite-difference time-domain (FDTD) method, and Fourier-transform infrared spectroscopy (FTIR). Through precision design of the micropatch array structure geometry, we achieve a significantly enhanced reflectance (85%), a substantial diffraction (10%), and a much reduced transmittance (5%) for an array of only 15% surface metal coverage. This results in an efficient far-field optical coupling with promising practical implications for efficient mid-infrared photodetectors. Most importantly we find that the propagating electromagnetic fields are transiently concentrated around the gold micropatch array in a time duration of tens of ns, providing us with a novel efficient near-field optical coupling.
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http://dx.doi.org/10.3390/s21155203DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8347955PMC
July 2021

Artificial Intelligence Pulse Coupled Neural Network Algorithm in the Diagnosis and Treatment of Severe Sepsis Complicated with Acute Kidney Injury under Ultrasound Image.

J Healthc Eng 2021 20;2021:6761364. Epub 2021 Jul 20.

Department of Emergency Medicine, Changzhou Cancer Hospital, Changzhou 213000, Jiangsu, China.

The objective of this study was to explore the diagnosis of severe sepsis complicated with acute kidney injury (AKI) by ultrasonic image information based on the artificial intelligence pulse coupled neural network (PCNN) algorithm. In this study, an algorithm of ultrasonic image information enhancement based on the artificial intelligence PCNN was constructed and compared with the histogram equalization algorithm and linear transformation algorithm. After that, it was applied to the ultrasonic image diagnosis of 20 cases of severe sepsis combined with AKI in hospital. The condition of each patient was diagnosed by ultrasound image performance, change of renal resistance index (RRI), ultrasound score, and receiver operator characteristic curve (ROC) analysis. It was found that the histogram distribution of this algorithm was relatively uniform, and the information of each gray level was obviously retained and enhanced, which had the best effect in this algorithm; there was a marked individual difference in the values of RRI. Overall, the values of RRI showed a slight upward trend after admission to the intensive care unit (ICU). The RRI was taken as the dependent variable, time as the fixed-effect model, and patients as the random effect; the parameter value of time was between 0.012 and 0.015, =0.000 < 0.05. Besides, there was no huge difference in the ultrasonic score among different time measurements ( = 1.348 and =0.128 > 0.05). The area under the ROC curve of the RRI for the diagnosis of AKI at the 2 day, 4 day, and 6 day was 0.758, 0.841, and 0.856, respectively, which was all greater than 0.5 ( < 0.05). In conclusion, the proposed algorithm in this study could significantly enhance the amount of information in ultrasound images. In addition, the change of RRI values measured by ultrasound images based on the artificial intelligence PCNN was associated with AKI.
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http://dx.doi.org/10.1155/2021/6761364DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8315850PMC
July 2021

Fragmenlt Recombination Design, Synthesis, and Safener Activity of Novel Ester-Substituted Pyrazole Derivatives.

J Agric Food Chem 2021 Aug 26;69(30):8366-8379. Epub 2021 Jul 26.

Department of Chemistry, College of Arts and Sciences, Northeast Agricultural University, Harbin 150030, China.

Fenoxaprop--ethyl (FE), a type of acetyl-CoA carboxylase (ACCase) inhibitor, has been extensively applied to a variety of crop plants. It can cause damage to wheat () even resulting in the death of the crop. On the prerequisite of not reducing herbicidal efficiency on target weed species, herbicide safeners selectively protect crops from herbicide injury. Based on fragment splicing, a series of novel substituted pyrazole derivatives was designed to ultimately address the phytotoxicity to wheat caused by FE. The title compounds were synthesized in a one-pot way and characterized infrared spectroscopy, H nuclear magnetic resonance, C nuclear magnetic resonance, and high-resolution mass spectrometry. The bioactivity assay proved that the FE phytotoxicity to wheat could be reduced by most of the title compounds. The molecular docking model indicated that compound prevented fenoxaprop acid (FA) from reaching or acting with ACCase. The absorption, distribution, metabolism, excretion, and toxicity predictions demonstrated that compound exhibited superior pharmacokinetic properties to the commercialized safener mefenpyr-diethyl. The current work revealed that a series of newly substituted pyrazole derivatives presented strong herbicide safener activity in wheat. This may serve as a potential candidate structure to contribute to the further protection of wheat from herbicide injury.
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http://dx.doi.org/10.1021/acs.jafc.1c02221DOI Listing
August 2021

Application and significance of PiCCO monitoring technique combined with troponin I detection in fluid resuscitation of elderly patients with septic myocardial dysfunction.

Am J Transl Res 2021 15;13(6):6846-6854. Epub 2021 Jun 15.

Department of Critical Care Medicine, Changzhou Fourth People's Hospital Changzhou 213000, Jiangsu Province, China.

Objective: This study was designed to demonstrate the predictive value of Pulse indicate Contour Cardiac Output (PiCCO) monitoring technique combined with troponin I (cTnI) detection in septic myocardial dysfunction (SMD) of the elderly.

Methods: One hundred and nineteen elderly patients with SMD treated in our hospital from March 2016 to September 2019 were enrolled and allocated into the joint group (JG; 64 cases) for capacity management of fluid resuscitation under the guidance of PiCCO monitoring technique and cTnI detection, and the control group (CG; 55 cases) for conventional capacity management. Clinical indicators, hemodynamics, improvement of myocardial injury markers and inflammatory factors 6 h and 36 h post intervention, fluid balance 6 h, 12 h and 36 h post intervention, drug consumption (norepinephrine), treatment effect and 28-day hospitalization mortality were compared between the two groups.

Results: After resuscitation, the urine volume per hour and the fluid resuscitation volume were higher while the blood lactic acid (BLA) expression was lower in JG as compared to CG. JG presented a remarkably lower central venous pressure (CVP) than CG after resuscitation, with notably higher mean arterial pressure (MAP) and central venous oxygen saturation (ScvO2). In comparison with CG, JG displayed dramatically lower cTnI and N-terminal pro-brain natriuretic peptide (NT-ProBNP) 6 h and 36 h post intervention, as well as evidently reduced interleukin-6 (IL-6), procalcitonin (PCT) and high-sensitivity C-reactive protein (hs-CRP). After 36 h of intervention, the fluid balance was evidently lower in JG than in CG. JG showed statistically less use of norepinephrine, less time of mechanical ventilation and ICU stay, and noticeably lower incidence of multiple organ dysfunction syndrome (MODS), as well as dramatically lower 28-day hospitalization mortality than CG post intervention.

Conclusions: PiCCO monitoring technique combined with cTnI detection is high-performing in fluid resuscitation of elderly patients with SMD, which can meliorate the myocardial function of patients, reduce medication and facilitate disease recovery.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290674PMC
June 2021

Mitochondrial Protection and Against Glutamate Neurotoxicity via Shh/Ptch1 Signaling Pathway to Ameliorate Cognitive Dysfunction by Kaixin San in Multi-Infarct Dementia Rats.

Oxid Med Cell Longev 2021 9;2021:5590745. Epub 2021 Jul 9.

Institute of Material Medica Integration and Transformation for Brain Disorders, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 611137, China.

Multi-infarct dementia (MID), a prominent subtype of vascular dementia (VD), is responsible for at least 15 to 20 percent of dementia in the elderly. Mitochondrial dysfunctions and glutamate neurotoxicity due to chronic hypoperfusion and oxidative stress were regarded as the major risk factors in the pathogenesis. (KXS), a classic prescription of , was applied to treatment for "amnesia" and has been demonstrated to alleviate the cognitive deficit in a variety of dementias, including MID. However, little is known whether mitochondria and glutamate are associated with the protection of KXS in MID treatment. The aim of this study was to investigate the role of KXS in improving the cognitive function of MID rats through strengthening mitochondrial functions and antagonizing glutamate neurotoxicity via the Shh/Ptch1 signaling pathway. Our data showed that KXS significantly ameliorated memory impairment and hippocampal neuron damage in MID rats. Moreover, KXS improved hippocampal mitochondrial functions by reducing the degree of mitochondrial swelling, increasing the mitochondrial membrane potential (MMP), and elevating the energy charge (EC) and ATP content in MID rats. As expected, the concentration of glutamate and the expression of p-NMDAR1 were significantly reduced by KXS in the brain tissue of MID rats. Furthermore, our results showed that KXS noticeably activated the Shh/Ptch1 signaling pathway which was demonstrated by remarkable elevations of Ptch1, Smo, and Gli1 protein levels in the brain tissue of MID rats. Intriguingly, the inhibition of the Shh signaling pathway with cyclopamine significantly inhibited the protective effects of KXS on glutamate-induced neurotoxicity in PC12 cells. To sum up, these findings suggested that KXS protected MID rats from memory loss by rescuing mitochondrial functions as well as against glutamate neurotoxicity through activating Shh/Ptch1 signaling pathway.
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http://dx.doi.org/10.1155/2021/5590745DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8285175PMC
July 2021

Phenoxypyridine derivatives containing natural product coumarins with allelopathy as novel and promising proporphyrin IX oxidase-inhibiting herbicides: Design, synthesis and biological activity study.

Pestic Biochem Physiol 2021 Aug 9;177:104897. Epub 2021 Jun 9.

Department of Chemistry, College of Arts and Sciences, Northeast Agricultural University, Harbin 150030, China. Electronic address:

To seek novel and safe protoporphyrinogen oxidase (PPO, EC 1.3.3.4) inhibitors with excellent herbicidal activity. A series of novel phenoxypyridine derivatives containing natural product coumarins with allelopathy were designed and synthesized based on bioisosterism and active subunit combination in this research. Compounds W3.1 and W3.4, with the half-maximal inhibitory concentration (IC) value of 0.02653 mg/L and 0.01937 mg/L, respectively, displayed excellent herbicidal activity in greenhouse. Their herbicidal activity was similar to commercial herbicide oxyfluorfen (IC = 0.04943 mg/L). The best field inhibitory effect of compounds W3.1 and W3.4 recorded was at doses of 450 g ai/ha and 300 g ai/ha, respectively. Compound W3.4 had the best herbicidal activity among all the target compounds in this paper. Molecular docking analysis revealed that compounds W3.1 and W3.4 could form a hydrogen bonds with the amino acid AGR-98 and a π-π superposition with the amino acid PHE-398, respectively, which was similar to the oxyfluorfen. The crop selectivity tests results indicated that maize, cotton and soybean showed high tolerance to compound W3.4. Compound W3.4 reduced the C and C contents of wheat and rice, but had less effect on maize, cotton and soybean. Selectivity of compound W3.4 in maize, cotton and soybean were appeared to be due to reduced absorption of the herbicide compared to wheat and rice. Compound W3.4 deserves further attention as a candidate structure for new herbicides.
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http://dx.doi.org/10.1016/j.pestbp.2021.104897DOI Listing
August 2021

Acinetobacter baumannii strains isolated from cerebrospinal fluid (CSF) and bloodstream analysed by cgMLST: the dominance of clonal complex CC92 in CSF infections.

Int J Antimicrob Agents 2021 Jul 21:106404. Epub 2021 Jul 21.

Department of Infectious Diseases, Sir Run Run Shaw Hospital, School of Medicine Zhejiang University, Hangzhou, China; and Key Laboratory of Microbial Technology and Bioinformatics of Zhejiang Province, Hangzhou, China. Electronic address:

A total of 54 unique clinical Acinetobacter baumannii strains isolated from cerebrospinal fluid (CSF) together with 21 bloodstream isolates collected from five tertiary hospitals in East China between April 2013 and November 2016 were studied for antimicrobial susceptibility patterns and the prevalence of antimicrobial resistance genes. Molecular epidemiological characteristics of CSF isolates and the phylogenetic relationship of isolates from different sources were assessed using multilocus sequence typing (MLST) and core genome MLST (cgMLST). Of the 54 CSF isolates, 51 (94.4%) were bla-carrying carbapenem-resistant A. baumannii. Their average resistance rate to different classes of antibiotics was extremely high (>90%), except for tigecycline and colistin. According to the Oxford MLST scheme, all CSF isolates fell into 10 defined sequence types (STs) and 4 novel STs. ST195 and ST208 were the leading STs in isolates from either source. A total of 50 CSF isolates and 20 bloodstream isolates were assigned to clonal complex 92 (CC92), revealing a wild distribution of CC92 in the hospitals of East China. In combination with epidemiological data linked in time and space, cgMLST results elucidated intrahospital and interhospital polyclonal dissemination of A. baumannii causing meningitis. Based on cgMLST, there was no correlation between phylogeny and the source of isolation of A. baumannii. These results emphasise that the genetic potential of this pathogen is vast enough to infect multiple human body sites.
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http://dx.doi.org/10.1016/j.ijantimicag.2021.106404DOI Listing
July 2021

A simple and rapid fluorescent approach for Pb determination and application in water samples and living cells.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Dec 10;263:120168. Epub 2021 Jul 10.

Department of Chemistry, College of Arts and Sciences, Northeast Agricultural University, Harbin, 150030, PR China. Electronic address:

A novel selective fluorescent chemosensor, thiosemicarbazide-appended naphthalimide derivative (TND), has been designed and synthesized, which exhibited good selectivity and sensibility for Pb in CHCN:HO (1:1) solution. The probe TND showed obvious color changes under UV light of 365 nm and displayed turn-on fluorescence response with Pb added. The binding mode of TND with Pb was found to be 1:1 based on the Job's plot analysis. The detection limit of Pb was 4.7 nM, which is far below the allowable concentration determined by WHO in drinking water. Moreover, the fortified recoveries of Pb were from 100.54% to 113.68% in water samples. TND is also applied for fluorescence imaging of Pb in lysosomes of human stromal cell line (HSC). This study indicated that TND would be a potential sensor detecting Pb in real sample.
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http://dx.doi.org/10.1016/j.saa.2021.120168DOI Listing
December 2021

Intron-targeted mutagenesis reveals roles for Dscam1 RNA pairing architecture-driven splicing bias in neuronal wiring.

Cell Rep 2021 Jul;36(2):109373

MOE Laboratory of Biosystems Homeostasis & Protection and Innovation Center for Cell Signaling Network, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang ZJ310058, China; Department of Neurosurgery, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang ZJ310058, China. Electronic address:

Drosophila melanogaster Down syndrome cell adhesion molecule (Dscam1) can generate 38,016 different isoforms through largely stochastic, yet highly biased, alternative splicing. These isoforms are required for nervous functions. However, the functional significance of splicing bias remains unknown. Here, we provide evidence that Dscam1 splicing bias is required for mushroom body (MB) axonal wiring. We generate mutant flies with normal overall protein levels and an identical number but global changes in exon 4 and 9 isoform bias (DscamΔ4D and DscamΔ9D), respectively. In contrast to DscamΔ4D, DscamΔ9D exhibits remarkable MB defects, suggesting a variable domain-specific requirement for isoform bias. Importantly, changes in isoform bias cause axonal defects but do not influence the self-avoidance of axonal branches. We conclude that, in contrast to the isoform number that provides the molecular basis for neurite self-avoidance, isoform bias may play a role in MB axonal wiring by influencing non-repulsive signaling.
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http://dx.doi.org/10.1016/j.celrep.2021.109373DOI Listing
July 2021

Tissue-specific expression of Cas9 has no impact on whole-body metabolism in four transgenic mouse lines.

Mol Metab 2021 Jul 8;53:101292. Epub 2021 Jul 8.

Baker Heart & Diabetes Institute, Melbourne, 3004, Australia; Baker Department of Cardiometabolic Health, The University of Melbourne, Melbourne, Australia; Central Clinical School, Monash University, Melbourne, 3004, Australia. Electronic address:

Objective: CRISPR/Cas9 technology has revolutionized gene editing and fast tracked our capacity to manipulate genes of interest for the benefit of both research and therapeutic applications. Whilst many advances have, and continue to be made in this area, perhaps the most utilized technology to date has been the generation of knockout cells, tissues and animals. The advantages of this technology are many fold, however some questions still remain regarding the effects that long term expression of foreign proteins such as Cas9, have on mammalian cell function. Several studies have proposed that chronic overexpression of Cas9, with or without its accompanying guide RNAs, may have deleterious effects on cell function and health. This is of particular concern when applying this technology in vivo, where chronic expression of Cas9 in tissues of interest may promote disease-like phenotypes and thus confound the investigation of the effects of the gene of interest. Although these concerns remain valid, no study to our knowledge has yet to demonstrate this directly.

Methods: In this study we used the lox-stop-lox (LSL) spCas9 ROSA26 transgenic (Tg) mouse line to generate four tissue-specific Cas9-Tg models that express Cas9 in the heart, liver, skeletal muscle or adipose tissue. We performed comprehensive phenotyping of these mice up to 20-weeks of age and subsequently performed molecular analysis of their organs.

Results: We demonstrate that Cas9 expression in these tissues had no detrimental effect on whole body health of the animals, nor did it induce any tissue-specific effects on whole body energy metabolism, liver health, inflammation, fibrosis, heart function or muscle mass.

Conclusions: Our data suggests that these models are suitable for studying the tissue specific effects of gene deletion using the LSL-Cas9-Tg model, and that phenotypes observed utilizing these models can be confidently interpreted as being gene specific, and not confounded by the chronic overexpression of Cas9.
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http://dx.doi.org/10.1016/j.molmet.2021.101292DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8361256PMC
July 2021

Corrigendum to "Dynamics of bacteriophages in gut of giant pandas reveal a potential regulation of dietary intake on bacteriophage composition" [Sci. Total Environ. 734 (2020) 139424].

Sci Total Environ 2021 Oct 1;789:148661. Epub 2021 Jul 1.

State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China. Electronic address:

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http://dx.doi.org/10.1016/j.scitotenv.2021.148661DOI Listing
October 2021

Conversion of marginal land into switchgrass conditionally accrues soil carbon but reduces methane consumption.

ISME J 2021 Jul 1. Epub 2021 Jul 1.

The Institute for Environmental Genomics, University of Oklahoma, Norman, OK, USA.

Switchgrass is a deep-rooted perennial native to the US prairies and an attractive feedstock for bioenergy production; when cultivated on marginal soils it can provide a potential mechanism to sequester and accumulate soil carbon (C). However, the impacts of switchgrass establishment on soil biotic/abiotic properties are poorly understood. Additionally, few studies have reported the effects of switchgrass cultivation on marginal lands that have low soil nutrient quality (N/P) or in areas that have experienced high rates of soil erosion. Here, we report a comparative analyses of soil greenhouse gases (GHG), soil chemistry, and microbial communities in two contrasting soil types (with or without switchgrass) over 17 months (1428 soil samples). These soils are highly eroded, 'Dust Bowl' remnant field sites in southern Oklahoma, USA. Our results revealed that soil C significantly increased at the sandy-loam (SL) site, but not at the clay-loam (CL) site. Significantly higher CO flux was observed from the CL switchgrass site, along with reduced microbial diversity (both alpha and beta). Strikingly, methane (CH) consumption was significantly reduced by an estimated 39 and 47% at the SL and CL switchgrass sites, respectively. Together, our results suggest that soil C stocks and GHG fluxes are distinctly different at highly degraded sites when switchgrass has been cultivated, implying that carbon balance considerations should be accounted for to fully evaluate the sustainability of deep-rooted perennial grass cultivation in marginal lands.
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http://dx.doi.org/10.1038/s41396-021-00916-yDOI Listing
July 2021

Clinical Features of COVID-19 Patients With Venous Thromboembolism.

Clin Appl Thromb Hemost 2021 Jan-Dec;27:10760296211013104

Department of Vascular Surgery, 89632Henan Provincial People's Hospital, the Affiliated People's Hospital of Zhengzhou University, the Affiliated People's Hospital of Henan University, Zhengzhou, Henan, China.

This study aimed to assess the clinical features of coronavirus disease 2019 (COVID-19) patients with VTE, to help develop preventive measures for venous thromboembolism (VTE in COVID-19) cases. COVID-19 patients admitted to Henan Provincial People's Hospital were retrospectively analyzed, including 23, 4 and 8 cases with mild to moderate, severe and critical symptoms, respectively. VTE incidence, age at onset, relevant laboratory parameters and prognosis were analyzed. Overall, VTE incidence in the 35 patients was 20.0%, occurring in severe (n = 1) and critical (n = 6) cases. D-dimer showed statistical significance in laboratory examination, representing except a diagnostic index and especial can be a prognostic factor in VTE among COVID-19 patients. Severe and critical COVID-19 cases had significantly reduced platelet counts, with a risk of hemorrhage. During treatment, the risk of both hemorrhage and thrombosis should be considered. VTE occurs in COVID-19 cases, affecting individuals with severe and critical symptoms. Significant D-dimer increase is of great significance in the risk assessment of death in critical cases of COVID-19. Appropriate measures should be taken to prevent VTE during treatment.
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http://dx.doi.org/10.1177/10760296211013104DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8236764PMC
July 2021

Design, synthesis, and herbicidal activity of novel phenoxypyridine derivatives containing natural product coumarin.

Pest Manag Sci 2021 Oct 6;77(10):4785-4798. Epub 2021 Jul 6.

Department of Chemistry, College of Arts and Sciences, Northeast Agricultural University, Harbin, China.

Background: In recent years, protoporphyrinogen oxidase (PPO, EC 1.3.3.4) inhibitors have been widely studied as important agricultural herbicides. Our research focused on the design and synthesis of novel PPO inhibitor herbicides, through linking of a diphenylether pyridine bioisostere structure to substituted coumarins, which aims to enhance environmental and crop safety while retaining high efficacy.

Results: A total of 21 compounds were synthesized via acylation reactions and all compounds were characterized using infrared, H NMR, C NMR, and high-resolution mass spectra. The respective configurations of compounds IV-6 and IV-12 were also confirmed using single crystal X-ray diffraction. The bioassay results showed that the title compounds displayed notable herbicidal activity, particularly compound IV-6 which displayed better herbicidal activity in greenhouse and field experiments, crop selectivity and safety for cotton and soybean compared with the commercial herbicide oxyfluorfen.

Conclusion: The work revealed that compound IV-6 deserves further attention as a candidate structure for a novel and safe herbicide. © 2021 Society of Chemical Industry.
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http://dx.doi.org/10.1002/ps.6523DOI Listing
October 2021

Deeply Infiltrating iRGD-Graphene Oxide for the Intensive Treatment of Metastatic Tumors through PTT-Mediated Chemosensitization and Strengthened Integrin Targeting-Based Antimigration.

Adv Healthc Mater 2021 08 16;10(16):e2100536. Epub 2021 Jun 16.

Department of Pharmaceutics, China Pharmaceutical University, 639 Longmian Avenue, Nanjing, 211198, China.

A limited infiltration and the subsequent low effective drug concentration result in poor chemotherapeutic outcomes against tumors, and even further promote tumor resistance and metastatic. Herein, iRGD-modified graphene oxide (GO) nanosheets (IPHG) are developed for the intensive treatment of metastatic tumors using focus-specific penetrated delivery together with photothermal therapy-mediated chemosensitization and photothermal therapy-strengthened integrin targeting-based antimigration. In vitro and in vivo data verified the mechanism of the tumor-selective infiltration of IPHG is based on a rigid 2D structure-associated advantage regarding hemodynamics and endothelial contact, followed by iRGD-endowed transendothelial and intratumoral transport. Once IPHG-DOX-penetrated 4T1 tumors are exposed to near-infrared irradiation, hyperthermia stress and photothermal therapy-elevated effective drug concentrations result in chemosensitization and prominent tumor suppression. Meanwhile, the specific binding of iRGD to integrins and photothermal therapy leads to the synergistic perturbation of cytoskeleton remodeling and subsequent impairment of cell motility and metastasis. The tailored design of IPHG validates a promising paradigm for drug delivery to combat tumor resistance and metastasis resulting from poor target access for single chemotherapy.
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http://dx.doi.org/10.1002/adhm.202100536DOI Listing
August 2021

Multifunctional peptide-assembled micelles for simultaneously reducing amyloid-β and reactive oxygen species.

Chem Sci 2021 Apr 13;12(18):6449-6457. Epub 2021 Apr 13.

Shanghai Engineering Research Center of Molecular Therapeutics and New Drug Development, East China Normal University Shanghai 200062 China

The excessive production and deposition of amyloid-β (Aβ) is one of the most important etiologies of Alzheimer's disease (AD). The interaction between Aβ and metal ions produces aberrant reactive oxygen species (ROS), which induce oxidative stress and accelerate the progression of AD. To reduce Aβ plaques and ROS to maintain their homeostasis is an emerging and ingenious strategy for effective treatment of AD. Herein, we report the rational design of multifunctional micelles (MPGLT) based on a polymer-grafted peptide to simultaneously clear Aβ and ROS for AD therapy. The MPGLT integrating three functional peptides as a ROS scavenger (tk-GSH), β-sheet breaker (LP) and an autophagy activator (TK) respectively, could capture and degrade Aβ. Meanwhile, the tk-GSH on the surface of MPGLT effectively scavenges the intracellular ROS. Consequently, MPGLT reduced the cytotoxicity of Aβ and ROS. animal studies using an AD mouse model further showed that MPGLT could transport across the blood-brain barrier for decreasing the Aβ plaque and eliminating ROS . This peptide micelle-based synergistic strategy may provide novel insight for AD therapy.
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http://dx.doi.org/10.1039/d1sc00153aDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8115327PMC
April 2021

Resistance evolution of hypervirulent carbapenem-resistant ST11 during treatment with tigecycline and polymyxin.

Emerg Microbes Infect 2021 Dec;10(1):1129-1136

Department of Infectious Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, People's Republic of China.

Hypervirulent carbapenem-resistant (hv-CRKP) has recently aroused increasing attention, especially ST11, the predominant CRKP clone in China. Here, we report a case of hv-CRKP-associated infection and reveal the in-host evolution of its mechanism of resistance to tigecycline and polymyxin under clinical therapy. A total of 11 carbapenemase (KPC)-producing CRKP strains were consecutively isolated from a male patient who suffered from continuous and multisite infections. String and antimicrobial susceptibility tests identified seven hypermucoviscous strains and three tigecycline-resistant and four colistin-resistant strains. larvae infection model confirmed the hypervirulence. Pulsed-field gel electrophoresis (PFGE) separated three PFGE clusters among all strains, and further Southern blotting detected that was located on the same-sized plasmid. Whole-genome sequencing showed that all strains belonged to the hv-CRKP ST11-KL64 clone. Diverse hypervirulence factors and resistance genes were identified. Further sequencing with the Nanopore platform was performed on the CRKP-Urine1 strain, which contained one virulence plasmid (pVi-CRKP-Urine1) and two resistance plasmids (pKPC-CRKP-Urine1 and pqnrS1-CRKP-Urine1). The gene mutations responsible for tigecycline or colistin resistance were then amplified with PCR followed by sequencing, which indicated that mutations of and were the potential loci for tigecycline resistance and that the , and genes for colistin resistance. A novel frameshift mutation of was identified in the high-level tigecycline-resistant strain (MIC, 128 mg/L). The results indicate that the hypervirulent ST11-KL64 clone is a potential threat to antiinfection treatment and is capable of rapid and diverse evolution of resistance during tigecycline and polymyxin treatment.
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http://dx.doi.org/10.1080/22221751.2021.1937327DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205050PMC
December 2021

[Landscape pattern of built-up land in a typical mining city, Datong, Shanxi, China from 1986 to 2018.]

Ying Yong Sheng Tai Xue Bao 2021 May;32(5):1614-1622

School of Earth Science and Surveying and Mapping Engineering, China University of Mining and Technology (Beijing), Beijing 100083, China.

Analyzing the landscape spatial layout and evolution characteristics of built-up land in a mining city is helpful to solve the environment problem, and can provide scientific basis for optimizing the urban land structure and taking timely intervention measures for transformation. Taking Datong, a typical mining city as the case, we examined the spatiotemporal characteristics of landscape of built-up land based on the spatial distribution data of built-up land from 1986 to 2018 with the landscape index analysis method, and detected the landscape change period with graphs obtained by Fourier transform and wavelet transform. The results showed that, during the study period, the area of built-up land in Datong City increased continually, with the growth rate gradually slowing down and the extent spreading from a "northeast-southwest" belt zone to the east. The fragmentation and complexity of the landscape pattern were increasing. The physical connectivity gradually decreased, while functional connectivity alternated between high and low values. The space expansion of Datong City and the fragmentation and complication of landscape pattern of its built-up land may all have a cycle of about 35 years, while the functional connectivity had a longer cycle than the physical connectivity.
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http://dx.doi.org/10.13287/j.1001-9332.202105.011DOI Listing
May 2021

Dynamic fingerprint of fractionalized excitations in single-crystalline CuZn(OH)FBr.

Nat Commun 2021 May 24;12(1):3048. Epub 2021 May 24.

Shenzhen Institute for Quantum Science and Engineering, and Department of Physics, Southern University of Science and Technology, Shenzhen, China.

Beyond the absence of long-range magnetic orders, the most prominent feature of the elusive quantum spin liquid (QSL) state is the existence of fractionalized spin excitations, i.e., spinons. When the system orders, the spin-wave excitation appears as the bound state of the spinon-antispinon pair. Although scarcely reported, a direct comparison between similar compounds illustrates the evolution from spinon to magnon. Here, we perform the Raman scattering on single crystals of two quantum kagome antiferromagnets, of which one is the kagome QSL candidate CuZn(OH)FBr, and another is an antiferromagnetically ordered compound EuCu(OH)Cl. In CuZn(OH)FBr, we identify a unique one spinon-antispinon pair component in the E magnetic Raman continuum, providing strong evidence for deconfined spinon excitations. In contrast, a sharp magnon peak emerges from the one-pair spinon continuum in the E magnetic Raman response once EuCu(OH)Cl undergoes the antiferromagnetic order transition. From the comparative Raman studies, we can regard the magnon mode as the spinon-antispinon bound state, and the spinon confinement drives the magnetic ordering.
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http://dx.doi.org/10.1038/s41467-021-23381-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144382PMC
May 2021

Electrospun Polymer-Free Nanofibers Incorporating Hydroxypropyl-β-cyclodextrin/Difenoconazole via Supramolecular Assembly for Antifungal Activity.

J Agric Food Chem 2021 Jun 20;69(21):5871-5881. Epub 2021 May 20.

Department of Chemistry, Northeast Agricultural University, Harbin 150030, China.

In this study, flexible and self-standing hydroxypropyl-β-cyclodextrin/difenoconazole inclusion complex (HPβCD/DZ-IC) nanofibers were prepared by polymer-free electrospinning, which exhibited potential to be a new fast-dissolving pesticide formulation. Scanning electron microscopy and optical microscopy were applied to evaluate the morphology of nanofibers, which showed that the resulting HPβCD/DZ-IC nanofibers were bead-free and uniform. In addition, the proton nuclear magnetic resonance (H NMR) spectrum suggested a stoichiometric ratio of 1:0.9 (HPβCD/DZ). Other characterization methods, such as UV-vis absorption, fluorescence spectroscopy, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and thermogravimetric analysis (TGA), were applied in this study. On the one hand, UV-vis absorption, fluorescence spectroscopy, FT-IR, XRD, and TGA provided useful information for the successful formation of an inclusion complex; on the other hand, the results of TGA indicated the thermal stability of DZ was enhanced after the formation of inclusion complexes. Besides, the phase solubility test could explain the increased water solubility of the nanofibers of inclusion complexes formed by DZ and HPβCD. The results of molecular docking studies demonstrated the most favorable binding interactions when HPβCD combined with DZ. The dissolution test and the antifungal performance test exhibited the characteristics of fast dissolution and the excellent antifungal performance of HPβCD/DZ-IC nanofibers, respectively.
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http://dx.doi.org/10.1021/acs.jafc.1c01351DOI Listing
June 2021

AtNUF2 modulates spindle microtubule organization and chromosome segregation during mitosis.

Plant J 2021 Aug 2;107(3):801-816. Epub 2021 Jun 2.

State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China.

The NDC80 complex is a conserved eukaryotic complex composed of four subunits (NUF2, SPC25, NDC80, and SPC24). In yeast and animal cells, the complex is located at the outer layer of the kinetochore, connecting the inner layer of the kinetochore and spindle microtubules (MTs) during cell division. In higher plants, the relationship of the NDC80 complex with MTs is still unclear. In this study, we characterized the biological function of AtNUF2, a subunit of the Arabidopsis NDC80 complex. We found that AtNUF2 is widely expressed in various organs, especially in different stages of embryonic development. It was verified that AtNUF2 co-localized with α-tubulin on MTs during mitosis by immunohistochemical assays. Mutation of AtNUF2 led to severe mitotic defects, not only in the embryo and endosperm, but also in seedlings, resulting in seed abortion and stagnating seedling growth. Furthermore, the biological function of AtNUF2 was studied using partially complemented nuf2-3/- (nuf2-3/- ) seedlings. The chromosome bridge and lagging chromatids occurred in nuf2-3/- root apical meristem cells, along with aberration of spindle MTs, resulting in blocked root growth. Meanwhile, the direct binding of AtNUF2 and AtSPC25 to MTs was determined by an MT co-sedimentation assay in vitro. This study revealed the function of AtNUF2 in mitosis and the underlying mechanisms, modulating spindle MT organization and ensuring chromosome segregation during embryo, endosperm, and root development, laying the foundation for subsequent research of the NDC80 complex.
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http://dx.doi.org/10.1111/tpj.15347DOI Listing
August 2021

Integrating Self-determination Theory and Upper Limb Factors to Predict Physical Activity in Patients With Breast Cancer During Chemotherapy.

Cancer Nurs 2021 Apr 21. Epub 2021 Apr 21.

Author Affiliations: Nursing School of Jilin University (Ms Fu, Dr Li Ms Wei, Ms Wang, Ms Dong, and Dr Peng); and Department of Breast Oncology, Jilin Provincial Cancer Hospital, Changchun, the People's Republic of China (Dr Zhou).

Background: Some empirical investigations try to describe the factors that affect the physical activity of breast cancer survivors, but these investigations rarely focus on the basic psychological needs or motivation regulation of breast cancer survivors in physical activity.

Objective: The aim of this study was to construct structural equation modeling of the self-determination theory and upper limb factors to predict the physical activity of breast cancer patients during chemotherapy.

Methods: A total of 236 breast cancer survivors completed measures of basic psychological needs, behavioral regulation in exercise, symptoms and dysfunction of upper limb factors, and physical activity. Path analyses structured the direct and indirect effects (via motivational orientations) of basic psychological needs and upper limb factors on physical activity.

Results: A total of 216 (91.5%) participants met the standard physical activity metabolic equivalent recommended. The final model has a good degree of fit (χ2 = 809.363, df = 391, χ2/df ≤ 2.07 [1-3]; root-mean-square error of approximation, 0.067 [<0.008]). The competence (0.309) of basic psychological needs and amotivation (-0.38) of motivation regulations have the greatest effect on physical activity.

Conclusions: Competence is the most important basic psychological need of breast cancer patients during chemotherapy, and amotivation is the most important motivation regulation.

Implications For Practice: Nurses need to pay more attention to the basic psychological needs and the motivation regulation in breast cancer patients during chemotherapy. Randomized controlled trials of breast cancer survivor interventions based on the self-determination theory are needed.
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http://dx.doi.org/10.1097/NCC.0000000000000951DOI Listing
April 2021
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