Publications by authors named "Ying Duan"

93 Publications

Diversity and seasonal abundance of Culicoides (Diptera, Ceratopogonidae) in Shizong County, Yunnan Province, China.

Parasite 2022 11;29:26. Epub 2022 May 11.

Yunnan Tropical and Subtropical Animal Virus Diseases Laboratory, Yunnan Animal Science and Veterinary Institute, Kunming 650224, Yunnan Province, China.

Culicoides (Diptera, Ceratopogonidae) are small biting midges, some of which are vectors for animal associated arboviruses such as bluetongue virus (BTV) and Akabane virus (AKAV). BTV and AKAV are both pathogenic for livestock, with BTV in particular posing a major threat to domestic ruminants. Ongoing problems with BTV in ruminants in Shizong County of Yunnan Province, China, promoted a year-long investigation of the Culicoides in Shizong to determine relative abundance and seasonality of midges and to attempt to identify species that might be acting as vectors in the area. Culicoides were collected by UV light trap for one night per week for most weeks between May 2020 and May 2021. More than 21,000 specimens consisting of at least 21 species belonging to six subgenera and one unplaced group, including 5 species previously associated with BTV and one associated with AKAV, were collected. Culicoides tainanus dominated most collections throughout the year although C. sumatrae was often the dominant species over summer. Most species were abundant between May and October. These results indicate that C. tainanus, C. jacobsoni and C. oxystoma are the major midge pests of livestock in Shizong and should be considered in any disease investigation.
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http://dx.doi.org/10.1051/parasite/2022027DOI Listing
May 2022

The relationship between disaster preparedness, psychological capital, and coping style among nurses: A cross-sectional study from China.

Perspect Psychiatr Care 2022 Apr 28. Epub 2022 Apr 28.

Department of Geriatrics, West China, Sichuan University, Chengdu, Wuhou, China.

Purpose: To find the relationship between psychological capital, coping style, and disaster preparedness in public hospital nurses.

Design And Methods: A cross-sectional study of 515 registered nurses from a public hospital in northeast Sichuan Province, China who were enrolled to complete self-reported questionnaires sent through the WeChat app.

Results: Disaster preparedness and psychology capital (r = 0.73, p < 0.01), disaster preparedness and coping style (r = 0.55, p < 0.01), and psychological capital and coping style (r = 0.56, p < 0.01) were positively correlated. Psychological capital (B = 1.81, p < 0.01) and coping style (B = 0.87, p < 0.01) accounted for 50% of disaster preparedness. Coping style partly mediated the effect of psychological capital on disaster preparedness (ab: 0.33, 95% confidence interval [CI]: 0.21-0.45).

Practical Implications: The level of psychological capital of nurses can provide an objective reference for administrators to establish intervention strategies and to promote positive psychological resources among nurses.
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http://dx.doi.org/10.1111/ppc.13097DOI Listing
April 2022

Non-invasive prenatal paternity testing by analysis of Y-chromosome mini-STR haplotype using next-generation sequencing.

PLoS One 2022 1;17(4):e0266332. Epub 2022 Apr 1.

Institute of Cytobiology, Dalian Medical University, Dalian, China.

Objectives: To assess the efficacy of Y-chromosome mini-STR-based next-generation sequencing (NGS) for non-invasive prenatal paternity testing (NIPPT).

Methods: DNA was extracted from the plasma of 24 pregnant women, and cell-free fetal DNA (cffDNA) haplotyping was performed at 12 Y-chromosome mini-STR loci using the Illumina NextSeq 500 system. The cffDNA haplotype was validated by the paternal haplotype. Subsequentlly, the paternity testing parameters were attributed to each case quantitatively.

Results: The biological relationship between the alleged fathers and infants in all 24 family cases were confirmed by capillary electrophoresis (CE). The Y-chromosome mini-STR haplotypes of all 14 male cffDNA were obtained by NGS without any missing loci. The alleles of cffDNA and paternal genomic DNA were matched in 13 cases, and a mismatched allele was detected at the DYS393 locus in one case and considered as mutation. No allele was detected in the 10 female cffDNA. The combined paternity index (CPI) and probability of paternity calculation was based on 6 loci Y-haplotype distributions of a local population. The probability of paternity was 98.2699-99.8828% for the cases without mutation, and 14.8719% for the case harboring mutation.

Conclusions: Our proof-of-concept study demonstrated that Y-chromosome mini-STR can be used for NGS-based NIPPT with high accuracy in real cases, and is a promising tool for familial searching, paternity exclusion and sex selection in forensic and medical applications.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0266332PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8974964PMC
April 2022

Tungsten Promoted Ni/AlO as a Noble-Metal-Free Catalyst for the Conversion of 5-Hydroxymethylfurfural to 1-Hydroxy-2,5-Hexanedione.

Front Chem 2022 9;10:857199. Epub 2022 Mar 9.

Henan Key Laboratory of Function-Oriented Porous Material, College of Chemistry and Chemical Engineering, Luoyang Normal University, Luoyang, China.

The conversion of 5-hydroxymethylfurfural (HMF) to 1-hydroxy-2,5-hexanedione (HHD) represented a typical route for high-value utilization of biomass. However, this reaction was often catalyzed by the noble metal catalyst. In this manuscript, W promoted Ni/AlO was prepared as a noble-metal-free catalyst for this transformation. The catalysts were characterized by XRD, XPS, NH-TPD, TEM, and EDS-mapping to study the influence of the introduction of W. There was an interaction between Ni and W, and strong acid sites were introduced by the addition of W. The W promoted Ni/AlO showed good selectivity to HHD when used as a catalyst for the hydrogenation of HMF in water. The influences of the content of W, temperature, H pressure, reaction time, and acetic acid (AcOH) were studied. NiWOx/AlO-0.5 (mole ratio of W:Ni = 0.5) was found to be the most suitable catalyst. The high selectivity to HHD was ascribed to the acid sites introduced by W. This was proved by the fact that the selectivity to HHD was increased a lot when AcOH was added just using Ni/AlO as catalysts. 59% yield of HHD was achieved on NiWOx/AlO-0.5 at 393 K, 4 MPa H reacting for 6 h, which was comparable to the noble metal catalyst, showing the potential application in the production of HHD from HMF.
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http://dx.doi.org/10.3389/fchem.2022.857199DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8959628PMC
March 2022

Predictive value of combining clinicopathological, multimodal ultrasonic characteristics in axillary lymph nodal metastasis burden of patients with cT1-2N0 breast cancer.

Clin Hemorheol Microcirc 2022 Mar 25. Epub 2022 Mar 25.

Department of Ultrasound, Gansu Cancer Hospital, Lanzhou, China.

Objective: To improving diagnosis of axillary lymph node metastasis (ALNM), we assessed the value of combining clinicopathological, conventional ultrasound, SWE features in the cT1-2N0 breast cancer patients.

Methods: Retrospective analysis of 285 patients with cT1-2N0 breast cancer who underwent preoperative ultrasound examination of the lesion and axillary, with shear wave elastography (SWE) of the lesions. According to the postoperative pathological results, they were divided into ≤2 metastatic ALNs group (low nodal burden, LNB) and > 2 metastatic ALNs group (high nodal burden, HNB). Binary logistic regression analysis was used to screen independent risk factors and establish prediction models. The best cut-off value of continuous variables is determined by the receiver operating characteristic curve, and the performance of the prediction model is evaluated.

Results: Presence of lymphovascular invasion (OR = 7.966, P = 0.010), tumor size (OR = 2.485, P = 0.019), Emean of intratumor (OR = 0.939, P = 0.002) and cortical thickness of lymph node (OR = 9.277, P < 0.001) were independent risk predictors for HNB of cT1-2N0 Group. The predictive model of combined method had better performance in predicting HNB of cT1-2N0 compared with models based on SWE and conventional ultrasound alone (area under the curve: 0.824 vs 0.658, P < 0.001; 0.824 vs 0.789, P = 0.035).

Conclusions: The predictive models of combined method obtained from significant clinicopathological and ultrasonographic features can potentially improve the diagnosis and individual treatment of ALNM in patients with cT1-2N0 breast cancer.
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http://dx.doi.org/10.3233/CH-221398DOI Listing
March 2022

Pyrolysis of Methyl Ricinoleate: Distribution and Characteristics of Fast and Slow Pyrolysis Products.

Materials (Basel) 2022 Feb 19;15(4). Epub 2022 Feb 19.

Biodiesel Engineering Lab of China Petroleum & Chemical Industry Federation, Zhejiang Province Key Lab of Biofuel, Zhejiang University of Technology, Hangzhou 310014, China.

A stable temperature site and the speed of heating the feedstocks play a key role in pyrolysis processes. In this study, the product distribution arising from pyrolysis of methyl ricinoleate (MR) at 550 °C with low and high heating rates was first studied by pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS). The results show that fast pyrolysis of MR favored the production of undecylenic acid methyl ester (UAME) and heptanal (HEP). Density functional theory (DFT) calculations were employed to reveal the UAME and HEP formation process from pyrolysis of MR. The bond dissociation energies (BDEs) of C-C bonds in MR showed that the C11-C12 bond is the weakest. This suggests that UAME and HEP are two major products. The process of slow and fast MR pyrolysis was the dehydration-first and the pyrolysis-first trend, respectively. The calculated activation energies of MR pyrolysis to UAME and HEP and MR dehydration to 9,12-octadecadienoic acid methyl ester were 287.72 and 238.29 kJ/mol, respectively. The much higher product yields obtained in the fast pyrolysis reactors than those from conventional tubular reactors confirmed the proposed process.
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http://dx.doi.org/10.3390/ma15041565DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8875455PMC
February 2022

Diagnosis and follow-up of glycogen storage disease (GSD) type VI from the largest GSD center in China.

Hum Mutat 2022 May 24;43(5):557-567. Epub 2022 Feb 24.

Department of Pediatric Endocrinology and Genetics, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Glycogen storage disease (GSD) Type VI is a glycogenolysis disorder caused by variants of PYGL. Knowledge about this disease is limited because only approximately 50 cases have been reported. We investigated the clinical profiles, molecular diagnosis, and treatment outcomes in patients with GSD VI from 2000 to 2021. The main initial clinical features of this cohort include hepatomegaly, short stature, elevated liver transaminases, hypertriglyceridemia, fasting hypoglycemia, and hyperuricemia. After uncooked cornstarch treatment, the stature and biochemical parameters improved significantly (p < 0.05). However, hyperuricemia recurred in most patients during adolescence. Among the 56 GSD VI patients, 54 biallelic variants and two single allelic variants of PYGL were identified, of which 43 were novel. There were two hotspot variants, c.1621-258_2178-23del and c.2467C>T p.(Gln823*), mainly in patients from Southwest and South China. c.1621-258_2178-23del is a 3.6 kb deletion that results in an out-of-frame deletion r.1621_2177del and an in-frame deletion r.1621_2265del. Our data show for the first time that long-term monitoring of uric acid is recommended for older GSD VI patients. This study also broadens the variant spectrum of PYGL and indicates that there are two hot-spot variants in China.
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http://dx.doi.org/10.1002/humu.24345DOI Listing
May 2022

SGK1 mutation status can further stratify patients with germinal center B-cell-like diffuse large B-cell lymphoma into different prognostic subgroups.

Cancer Med 2022 03 1;11(5):1281-1291. Epub 2022 Feb 1.

Department of Chemotherapy, Guangxi Medical University Affiliated Tumor Hospital, Nanning, China.

There are over a 100 driver gene mutations in patients with diffuse large B-cell lymphoma (DLBCL), but their clinical significance remains unclear. Here, we first analyzed the DLBCL dataset from the UK-based Haematological Malignancy Research Network. Patients were divided into high- and low-risk groups based on whether lymphoma progressed within 24 months. Genes showing significantly different frequencies between groups were selected. Survival data for patients with the selected mutant genes were analyzed. The results were validated using two other large databases to evaluate the relationship between the selected mutant genes and prognosis. The mutation frequencies of 11 genes (MYD88[L265P], SGK1, MPEG1, TP53, SPEN, NOTCH1, ETV6, TNFRSF14, MGA, CIITA, and PIM1) significantly differed between the high- and low-risk groups. The relationships between these mutant genes and patient survival were analyzed. Patients who harbored SGK1 (serum and glucocorticoid-inducible kinase 1) mutations exhibited the best prognosis. Most patients with SGK1 mutation are germinal center B-cell (GCB) subtype. Among patients with GCB DLBCL, those harboring SGK1 mutations exhibited better prognosis than those without SGK1 mutations. Most SGK1 mutations were single-base substitutions, primarily scattered throughout the catalytic domain-encoding region. Multiple SGK1 mutations were identified in a single patient. Thus, SGK1 mutations are a marker of good prognosis for DLBCL and occur predominantly in the GCB subtype of DLBCL. SGK1 mutation status can further stratify patients with GCB DLBCL into different prognostic subgroups.
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http://dx.doi.org/10.1002/cam4.4550DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8894717PMC
March 2022

Promoter variation in a homeobox gene, CpDll, is associated with deeply lobed leaf in Cucurbita pepo L.

Theor Appl Genet 2022 Apr 5;135(4):1223-1234. Epub 2022 Jan 5.

Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture and Rural Affairs, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.

Key Message: CpDll, encoding an HD-Zip I transcription factor, positively regulates formation of deeply lobed leaf shape in zucchini, Cucurbita pepo, which is associated with sequence variation in its promoter region. Leaf shape is an important horticultural trait in zucchini (Cucurbita pepo L.). Deeply lobed leaves have potential advantages for high-density planting and hybrid production. However, little is known about the molecular basis of deeply lobed leaf formation in this important vegetable crop. Here, we conducted QTL analysis and fine mapping of the deeply lobed leaf (CpDll) locus using recombinant inbred lines and large F populations developed from crosses between the deeply lobed leaf HM-S2, and entire leaf Jin-GL parental lines. We show that CpDll exhibited incomplete dominance for the deeply lobed leaf shape in HM-S2. Map-based cloning provided evidence that CpCll encodes a type I homeodomain (HD)- and Leu zipper (Zip) element-containing transcription factor. Sequence analysis between HM-S2 and Jin-GL revealed no sequence variations in the coding sequences, whereas a number of variations were identified in the promoter region between them. DUAL-LUC assays revealed significantly stronger promoter activity in HM-S2 than that in Jin-GL. There was also significantly higher expression of CpDll in the leaf base of deeply lobed leaves of HM-S2 compared with entire leaf Jin-GL. Comparative analysis of CpDll gene homologs in nine cucurbit crop species (family Cucurbitaceae) revealed conservation in both structure and function of this gene in regulation of deeply lobed leaf formation. Our work provides new insights into the molecular basis of leaf lobe formation in pumpkin/squash and other cucurbit crops. This work also facilitates marker-assisted selection for leaf shape in zucchini breeding.
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http://dx.doi.org/10.1007/s00122-021-04026-3DOI Listing
April 2022

Clinical, genetic profile and therapy evaluation of 55 children and 5 adults with sitosterolemia.

J Clin Lipidol 2022 Jan-Feb;16(1):40-51. Epub 2021 Dec 6.

Department of Pediatric Endocrinology and Genetic Metabolism, Xinhua Hospital, Shanghai Institute of Pediatric Research, School of Medicine, Shanghai Jiao Tong University, 1665 Kong Jiang Road, Shanghai 200092, China. Electronic address:

Background: Sitosterolemia is a rare autosomal recessive disease characterized by phytosterol accumulation in the blood and tissues. However, the detailed clinical and genetic spectra are lacking.

Objective: To describe and compare the clinical, biochemical, genetic, therapeutic, and follow-up characteristics of 55 pediatric and five adult sitosterolemia patients.

Methods: Clinical, genetic and therapeutic data from 60 patients at Xinhua Hospital from January 2016 to June 2021 were retrospectively collected.

Results: Pediatric patients' manifestations included xanthomas(93%), hematological disorders(30%), arthralgia(24%), splenomegaly(11%), atherosclerosis(10%). Adult patients had symptoms such as atherosclerosis(5/5), xanthomas(4/5), hematological disorders(3/5), arthralgia(3/5), splenomegaly(3/5). Elevated total cholesterol(TC) and low-density lipoprotein cholesterol(LDL-C) were observed in 96% patients (pediatric 98%, adult 3/4), and phytosterol levels in 100% patients. The age of onset was also negatively correlated with blood TC (P < 0.0001, r = -0.5548) and LDL-C (P = 0.0001, r = -0.4859) levels. Targeted treatments resulted in symptomatic remission(pediatric 96%, adult 4/5), and significantly decreased lipid and phytosterol levels(all P<0.05). In the dietary-therapy cohort(n=34), blood lipid levels decreased(all P<0.05). In the 13 pediatric patients from the dietary-therapy cohort who switched from dietary to combination therapy with ezetimibe, dietary therapy decreased TC and LDL-C levels by 54% and 52%, and ezetimibe further decreased them by 18% and 20%, respectively. Further, we identified 15 novel ABCG5/ABCG8 variants.

Conclusions: This study expands the clinical and genetic spectra of sitosterolemia. The low-phytosterol diet is the cornerstone of sitosterolemia treatment. Ezetimibe can further decrease blood lipid levels and increase daily dietary phytosterol tolerance.
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http://dx.doi.org/10.1016/j.jacl.2021.11.015DOI Listing
April 2022

Analysis of Salivary Microbiome and Its Association With Periodontitis in Patients With Obstructive Sleep Apnea.

Front Cell Infect Microbiol 2021 7;11:752475. Epub 2021 Dec 7.

Department of Orthodontics, Peking University School and Hospital of Stomatology, Beijing, China.

Objectives: This study aimed to analyze the periodontal conditions of patients with obstructive sleep apnea (OSA) in relation to the salivary microbiome.

Materials And Methods: In total, 54 male adults (27 with OSA, 27 controls) completed this cross-sectional study. All participants were monitored by overnight polysomnography (PSG) and underwent full-mouth periodontal examination. Saliva samples were then collected, and the microbial 16S ribosomal RNA gene was sequenced. The data were analyzed to determine the microbial distribution and the community structure of the two groups.

Results: Demonstrated by alpha and beta diversity, the OSA group had a lower microbial richness and a lower observed species than the controls. There was no significant difference in the microbial species diversity or evenness between the OSA and the non-OSA groups. The OSA group had fewer operational taxonomic units (OTUs), and the distribution of microbiome showed that several gram-positive bacteria had higher abundance in the OSA group. As for periodontal pathogens, the relative abundance of was significantly increased in the OSA group. No significant difference was observed in the relative abundance of other pathogens at either the genus or species level.

Conclusions: The salivary microbial community structure was altered in patients with OSA in terms of species richness and trans-habitat diversity, along with an increase in , a specific periodontal pathogen. These findings might explain the high prevalence of periodontitis in OSA patients.
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http://dx.doi.org/10.3389/fcimb.2021.752475DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8688821PMC
January 2022

Ultrasound-based radiomics: current status, challenges and future opportunities.

Med Ultrason 2021 Nov 11. Epub 2021 Nov 11.

Ultrasound Medicine Center, Lanzhou University Second Hospital, Lanzhou, Gansu, China.

Ultrasound (US) imaging is part of conventional medical imaging in clinical practice that is low-cost, non-ionizing, portable and capable of real-time image acquisition and display. However, in certain cases, US has limited sensitivity and specificity in differentiating between malignant and benign lesions. Ultrasound-based radiomics, as a new branch of radiomics, can provide additional features such as heterogeneity of lesions that are invisible to the naked eye, alone or in combination with demographic, histological, genomic or proteomic data, thereby improving the accuracy of US in diagnosis of disease. This article provides an introduction to ultrasound-based radiomics, covering its workflow, the application of machine learning, and current research status. Current limitations of radiomics, such as consistency of image acquisition, parameter variations, and difficulty in calibrating quantitative methods in ultrasound, will also be covered.
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http://dx.doi.org/10.11152/mu-3248DOI Listing
November 2021

Orbitofrontal cortex and dorsal striatum functional connectivity predicts incubation of opioid craving after voluntary abstinence.

Proc Natl Acad Sci U S A 2021 10;118(43)

Neuroimaging Research Branch, Intramural Research Program/National Institute on Drug Abuse/NIH, Baltimore, MD 21224

We recently introduced a rat model of incubation of opioid craving after voluntary abstinence induced by negative consequences of drug seeking. Here, we used resting-state functional MRI to determine whether longitudinal functional connectivity changes in orbitofrontal cortex (OFC) circuits predict incubation of opioid craving after voluntary abstinence. We trained rats to self-administer for 14 d either intravenous oxycodone or palatable food. After 3 d, we introduced an electric barrier for 12 d that caused cessation of reward self-administration. We tested the rats for oxycodone or food seeking under extinction conditions immediately after self-administration training (early abstinence) and after electric barrier exposure (late abstinence). We imaged their brains before self-administration and during early and late abstinence. We analyzed changes in OFC functional connectivity induced by reward self-administration and electric barrier-induced abstinence. Oxycodone seeking was greater during late than early abstinence (incubation of oxycodone craving). Oxycodone self-administration experience increased OFC functional connectivity with dorsal striatum and related circuits that was positively correlated with incubated oxycodone seeking. In contrast, electric barrier-induced abstinence decreased OFC functional connectivity with dorsal striatum and related circuits that was negatively correlated with incubated oxycodone seeking. Food seeking was greater during early than late abstinence (abatement of food craving). Food self-administration experience and electric barrier-induced abstinence decreased or maintained functional connectivity in these circuits that were not correlated with abated food seeking. Opposing functional connectivity changes in OFC with dorsal striatum and related circuits induced by opioid self-administration versus voluntary abstinence predicted individual differences in incubation of opioid craving.
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http://dx.doi.org/10.1073/pnas.2106624118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8639358PMC
October 2021

Isolation of Tibet Orbivirus from Culicoides jacobsoni (Diptera, Ceratopogonidae) in China.

Parasit Vectors 2021 Aug 28;14(1):432. Epub 2021 Aug 28.

Yunnan Tropical and Subtropical Animal Virus Diseases Laboratory, Yunnan Animal Science and Veterinary Institute, Kunming, Yunnan, China.

Background: Tibet Orbivirus (TIBOV) is a recently discovered Orbivirus known to infect cattle, Asian buffalo and goats in south-western China. It was first isolated from mosquitoes and subsequently from biting midges (Culicoides spp.) in Yunnan, China, indicating that it is an arbovirus. Little is known of its potential to cause disease, but the economic importance of related viruses promoted an investigation of potential Culicoides spp. vectors of TIBOV.

Methods: Biting midges were collected approximately once per week between May and December 2020, at a cattle farm in Wulong village, Shizong County, Yunnan Province, China. Approximately 3000 specimens of nine species were subsequently used in attempts to isolate virus, and a further 2000 specimens of six species were tested for the presence of bluetongue virus (BTV) and TIBOV using a RT-qPCR test.

Results: Virus isolation attempts resulted in the isolation of three viruses. One isolate from a pool of Culicoides jacobsoni was identified as TIBOV, while the other two viruses from C. orientalis and C. tainanus remain unidentified but are not BTV or TIBOV. RT-qPCR analysis did not detect BTV in any specimens, but a single pool containing five specimens of C. jacobsoni and another containing five specimens of C. tainanus produced PCR quantification cycle (Cq) values of around 28 that may indicate infection with TIBOV.

Conclusions: The isolation of TIBOV from C. jacobsoni satisfies one criterion required to prove its status as a vector of this virus. This isolation is supported by a low Cq value produced from a different pool of this species in the RT-qPCR test. The low Cq value obtained from a pool of C. tainanus suggests that this species may also be able to satisfy this criterion. Both of these species are widespread throughout Asia, with C. jacobsoni extending into the Pacific region, which raises the possibility that TIBOV may be more widespread than is currently known.
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http://dx.doi.org/10.1186/s13071-021-04899-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401062PMC
August 2021

Nighttime Temperatures and Sunlight Intensities Interact to Influence Anthocyanin Biosynthesis and Photooxidative Sunburn in "Fuji" Apple.

Front Plant Sci 2021 23;12:694954. Epub 2021 Jul 23.

State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Xianyang, China.

Light and low temperatures induce anthocyanin accumulation, but intense sunlight causes photooxidative sunburn. Nonetheless, there have been few studies of anthocyanin synthesis under different sunlight intensities and low nighttime temperatures. Here, low nighttime temperatures followed by low light intensity were associated with greater anthocyanin accumulation and the expression of anthocyanin biosynthesis genes in "Fuji" apple peel. UDP-glucose flavonoid-3-O-glucosyltransferase (UFGT) activity was positively associated with anthocyanin enrichment. Ascorbic acid can be used as an electron donor of APX to scavenge HO in plants, which makes it play an important role in oxidative defense. Exogenous ascorbate altered the anthocyanin accumulation and reduced the occurrence of high light-induced photooxidative sunburn by removing hydrogen peroxide from the peel. Overall, low light intensity was beneficial for the accumulation of anthocyanin and did not cause photooxidative sunburn, whereas natural light had the opposite effect on the apple peel at low nighttime temperatures. This study provides an insight into the mechanisms by which low temperatures induce apple coloration and high light intensity causes photooxidative sunburn.
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http://dx.doi.org/10.3389/fpls.2021.694954DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8343144PMC
July 2021

A Flavoprotein Dioxygenase Steers Bacterial Tropone Biosynthesis via Coenzyme A-Ester Oxygenolysis and Ring Epoxidation.

J Am Chem Soc 2021 07 1;143(27):10413-10421. Epub 2021 Jul 1.

Faculty of Biology, University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany.

Bacterial tropone natural products such as tropolone, tropodithietic acid, or the roseobacticides play crucial roles in various terrestrial and marine symbiotic interactions as virulence factors, antibiotics, algaecides, or quorum sensing signals. We now show that their poorly understood biosynthesis depends on a shunt product from aerobic CoA-dependent phenylacetic acid catabolism that is salvaged by the dedicated acyl-CoA dehydrogenase-like flavoenzyme TdaE. Further characterization of TdaE revealed an unanticipated complex catalysis, comprising substrate dehydrogenation, noncanonical CoA-ester oxygenolysis, and final ring epoxidation. The enzyme thereby functions as an archetypal flavoprotein dioxygenase that incorporates both oxygen atoms from O into the substrate, most likely involving flavin-N5-peroxide and flavin-N5-oxide species for consecutive CoA-ester cleavage and epoxidation, respectively. The subsequent spontaneous decarboxylation of the reactive enzyme product yields tropolone, which serves as a key virulence factor in rice panicle blight caused by pathogenic edaphic . Alternatively, the TdaE product is most likely converted to more complex sulfur-containing secondary metabolites such as tropodithietic acid from predominant marine (e.g., ).
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http://dx.doi.org/10.1021/jacs.1c04996DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8283759PMC
July 2021

FDG PET/CT in Tenosynovial Giant Cell Tumor of the Lumbar Facet Joint.

Clin Nucl Med 2022 Jan;47(1):66-67

From the Shanghai University, Shanghai Universal Medical Imaging Diagnostic Center.

Abstract: Tenosynovial giant cell tumor rarely affects the facet joints of the spine. We describe FDG PET/CT findings in a case of tenosynovial giant cell tumor arising from the left L2 to L3 facet joint. The tumor caused osteolytic bone destruction of the facet joint and showed intense FDG uptake with SUVmax of 10.4. This case indicates tenosynovial giant cell tumor should be included in the differential diagnosis of abnormal facet joint FDG accumulation.
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http://dx.doi.org/10.1097/RLU.0000000000003729DOI Listing
January 2022

Triazole/thiadiazole substituted 4'-demethylepipodophyllotoxin derivatives induced apoptosis in HeLa cells by up-regulating TMEM133.

Eur J Pharmacol 2021 Aug 24;905:174189. Epub 2021 May 24.

State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, China. Electronic address:

Heterocycle modification has been widely and successfully employed in the antitumor drugs. However, the different antitumor efficacy was corelated with the heterocycle substituted, and the genetic mechanism underlying these effects has not been elucidated. In this study, the intrinsic regularity between different types of heterocycle-substituted DMEP derivative compounds and the mechanisms of their antitumor activity was preliminarily disclosed. Triazole/thiadiazole substituted 4'-demethylepipodophyllotoxin derivatives induced more severe DNA damage and higher levels of 26S proteasomal Topo IIβ degradation, though inhibited the recruition of γH2AX to resist the DNA damage. The reduced DNA repair led to higher up-regulation of cell cycle arrest proteins, and ultimately DNA damage mediated-ATM/ATR apoptotic pathways and specifically activated DNA damage response gene TMEM133, which induced apoptosis through up-regulation of G/M cell cycle arrest-related genes. Over-expression and knock-out of TMEM133 demonstrated that TMEM133 is essential for inhibition of the tumor cell growth during treatment with triazole/thiadiazole substituted 4'-demethylepipodophyllotoxin derivatives.
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http://dx.doi.org/10.1016/j.ejphar.2021.174189DOI Listing
August 2021

Increased cocaine motivation in tree shrews is modulated by striatal dopamine D1 receptor-mediated upregulation of Ca 1.2.

Addict Biol 2021 11 13;26(6):e13053. Epub 2021 May 13.

CAS Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing, China.

The progressively increased motivation for cocaine during abstinence is closely associated with the dysfunction of dopamine (DA) system. As DA receptors also dynamically regulate L-type calcium channels (LTCCs), in this study we examined how DA receptors (D1R or D2R) and LTCCs (Ca 1.2 or Ca 1.3) exert their influences on cocaine-seeking in a tree shrew (Tupaia belangeri chinensis) model. First, we demonstrated the 'incubation' effect by showing tree shrews exhibited a significantly higher seeking behaviour on withdrawal day (WD) 45 than on WD1. Then, we confirmed that longer abstinence period induced higher D1R expression in the nucleus accumbens (NAc). Next, we showed that LTCCs in the NAc participated in drug seeking. Moreover, Ca 1.2 expression in the NAc was increased on WD45, and disruption of the Ca 1.2 inhibited drug seeking. Finally, we found that D1R antagonist blocked the increase of Ca 1.2 on drug-seeking test. Collectively, these findings suggest that D1R-mediated upregulation of Ca 1.2 is involved in the incubation of cocaine craving.
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http://dx.doi.org/10.1111/adb.13053DOI Listing
November 2021

Genetic Mapping and Identification of the Candidate Gene for White Seed Coat in .

Int J Mol Sci 2021 Mar 15;22(6). Epub 2021 Mar 15.

Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of Ministry of Agriculture and Rural Affairs, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.

Seed coat color is an important agronomic trait of edible seed pumpkin in . In this study, the development pattern of seed coat was detected in yellow and white seed coat accessions Wuminglv and Agol. Genetic analysis suggested that a single recessive gene () is involved in seed coat color regulation in . An F segregating population including 2798 plants was used for fine mapping and a candidate region containing nine genes was identified. Analysis of 54 inbred accessions revealed four main Insertion/Deletion sites in the promoter of encoding an MYB transcription factor were co-segregated with the phenotype of seed coat color. RNA-seq analysis and qRT-PCR revealed that some genes involved in phenylpropanoid/flavonoid metabolism pathway displayed remarkable distinction in Wuminglv and Agol during the seed coat development. The flanking InDel marker S1548 was developed to predict the seed coat color in the MAS breeding with an accuracy of 100%. The results may provide valuable information for further studies in seed coat color formation and structure development in Cucurbitaceae crops and help the molecular breeding of .
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http://dx.doi.org/10.3390/ijms22062972DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000038PMC
March 2021

Widely Targeted Metabolomics Analysis of Different Parts of Pall.

Molecules 2021 Feb 20;26(4). Epub 2021 Feb 20.

School of Food and Drug, College of Life Science, Luoyang Normal University, Jiqing Road 6, Luoyang 471934, China.

Pall has a long history of being used as a traditional medicine to treat hypertension, headache, insomnia, constipation and vertigo. However, only a few biologically active substances have been identified from . Here, the shoots and roots of , namely L-Sc and R-Sc, were studied. The primary and secondary metabolites were investigated using ultrahigh-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS). A total of 637 putative metabolites were identified and these metabolites were mainly classified into ten different categories. Correlation analysis, hierarchical clustering analysis, principal component analysis and orthogonal partial least squares discriminant analysis of metabolites showed that the L-Sc samples could be clearly separated from the R-Sc samples. Differential accumulated metabolite analysis revealed that most of differential primary metabolites were significantly lower in the L-Sc than in the R-Sc. Conversely, the major differential secondary metabolites had higher levels in the L-Sc than in the R-Sc. Further analysis indicated that the flavonoids were the major putative antioxidant components and most of putative antioxidant components exhibited higher relative concentrations in the L-Sc than the R-Sc. These results improve our understanding of metabolite accumulation and provide a reference for the study of medicinal value in .
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http://dx.doi.org/10.3390/molecules26041126DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7924207PMC
February 2021

Transcriptional control of local auxin distribution by the CsDFB1-CsPHB module regulates floral organogenesis in cucumber.

Proc Natl Acad Sci U S A 2021 02;118(8)

Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, 100193 Beijing, China;

Plant cystatins are cysteine proteinase inhibitors that play key roles in defense responses. In this work, we describe an unexpected role for the cystatin-like protein DEFORMED FLORAL BUD1 (CsDFB1) as a transcriptional regulator of local auxin distribution in cucumber ( L.). was strongly expressed in the floral meristems, floral primordia, and vasculature. RNA interference (RNAi)-mediated silencing of led to a significantly increased number of floral organs and vascular bundles, together with a pronounced accumulation of auxin. Conversely, accompanied by a decrease of auxin, overexpression of resulted in a dramatic reduction in floral organ number and an obvious defect in vascular patterning, as well as organ fusion. CsDFB1 physically interacted with the cucumber ortholog of PHABULOSA (CsPHB), an HD-ZIP III transcription factor whose transcripts exhibit the same pattern as Overexpression of increased auxin accumulation in shoot tips and induced a floral phenotype similar to that of RNAi lines. Furthermore, genetic and biochemical analyses revealed that CsDFB1 impairs CsPHB-mediated transcriptional regulation of the auxin biosynthetic gene and the auxin efflux carrier , and thus plays a pivotal role in auxin distribution. In summary, we propose that the CsDFB1-CsPHB module represents a regulatory pathway for local auxin distribution that governs floral organogenesis and vascular differentiation in cucumber.
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http://dx.doi.org/10.1073/pnas.2023942118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923377PMC
February 2021

Detection of bluetongue virus in Culicoides spp. in southern Yunnan Province, China.

Parasit Vectors 2021 Jan 22;14(1):68. Epub 2021 Jan 22.

Yunnan Tropical and Subtropical Animal Virus Diseases Laboratory, Yunnan Animal Science and Veterinary Institute, Kunming, Yunnan Province, China.

Background: Culicoides (Diptera: Ceratopogonidae) are vectors for many arboviruses. At least 20 species are considered as vectors or potential vectors of bluetongue virus (BTV) which cause bluetongue disease in ruminants. A BTV prevalence of 30-50% among cattle and goats in tropical southern Yunnan Province, China, prompted an investigation of the potential BTV vectors in this area.

Methods: Culicoides were collected by light trapping at three sites in the tropical region of Yunnan Province. Species were identified based on morphology and DNA sequences of cytochrome c oxidase subunit 1 (cox1). PCR and quantitative PCR following reverse transcription were used to test for the presence of BTV RNA in these specimens. Phylogenetic analysis was used to analyze the cox1 sequences of Culicoides specimens infected with BTV.

Results: Approximately 67,000 specimens of Culicoides were collected, of which 748 were tested for the presence of BTV. Five specimens, including two of Culicoides jacobsoni, one of C. tainanus and two of C. imicola, were identified as infected with BTV. No specimens of C. (subgenus Trithecoides) or C. oxystoma tested were positive for BTV infection.

Conclusions: To our knowledge this is the first report of C. jacobsoni as a potential BTV vector and the fourth report of an association between C. tainanus and BTV, as well as the first direct evidence of an association between BTV and C. imicola in Asia. A fourth potential cryptic species within C. tainanus was identified in this study. Further analysis is required to confirm the importance of C. jacobsoni and C. tainanus in BTV epidemiology in Asia.
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http://dx.doi.org/10.1186/s13071-020-04518-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7821528PMC
January 2021

Effects of Hemodialysis and Peritoneal Dialysis on Glycometabolism in Patients with End-Stage Diabetic Nephropathy.

Blood Purif 2021 10;50(4-5):506-512. Epub 2020 Dec 10.

Blood Purification Center, Nanjing First Hospital, Nanjing Medical University, Nanjing, China,

Objective: The objective is to study the fluctuation pattern of blood glucose spectrum in patients with end-stage diabetic nephropathy (ESDN) receiving hemodialysis (HD) and peritoneal dialysis (PD), respectively, and to compare the influences of these 2 dialysis methods on glycometabolism.

Methods: Sixty-four dialysis patients with ESDN were recruited, including 35 HD patients and 29 PD patients. The 24-h blood glucose on dialysis days of the 2 groups was monitored by the continuous glucose monitoring system, and the relevant glycometabolism indexes were recorded and compared.

Results: The control of blood glucose in both groups was not satisfactory. At the same blood glucose level, the dosage of exogenous insulin needed by patients in the PD group was larger than that in the HD group (p < 0.05). However, the fluctuation of blood glucose and consequently the incidence of hyperglycemia and hypoglycemia in HD group were greater than that in PD group (p < 0.05). The patients' blood glucose levels decreased progressively during the course of HD. The mean blood glucose (MBG) values estimated by glycosylated hemoglobin (HbA1c) in both groups were lower than the actual measured blood glucose values (p < 0.05). Preliminary correlation analysis showed that the deviation of the MBG values estimated by HbA1c was positively correlated with the degree of anemia.

Conclusion: HD patients have larger glycemic variability as compared with PD patients, while PD patients have overall increased blood glucose levels. Hypoglycemic programs should be made according to the corresponding changes in blood glucose. The HbA1c value of dialysis patients has a large deviation, so it is necessary to explore its influencing factors and develop more accurate blood glucose assessment indicators.
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http://dx.doi.org/10.1159/000511722DOI Listing
January 2022

Real-World Effectiveness of Direct-Acting Antiviral Regimens against Hepatitis C Virus (HCV) Genotype 3 Infection: A Systematic Review and Meta-Analysis.

Ann Hepatol 2021 Jul-Aug;23:100268. Epub 2020 Oct 12.

Center of Liver Disease Division 3, Beijing Ditan Hospital, Capital Medical University, Beijing, China; Peking University Ditan Teaching Hospital, Beijing, China. Electronic address:

Patients with hepatitis C virus (HCV) genotype 3 (GT3) infection are resistant to direct-acting antiviral (DAA) treatments. This study aimed to analyze the effectiveness of sofosbuvir (SOF)+daclatasvir (DCV) ± ribavirin (RBV); SOF+velpatasvir (VEL)±RBV; SOF+VEL+voxilaprevir (VOX); and glecaprevir (GLE)+pibrentasvir (PIB) in the treatment of HCV GT3-infected patients in real-world studies. Articles were identified by searching the PubMed, EMBASE, and Cochrane Library databases from January 1, 2016 to September 10, 2019. The meta-analysis was conducted to determine the sustained virologic response (SVR) rate, using R 3.6.2 software. Thirty-four studies, conducted on a total of 7328 patients from 22 countries, met the inclusion criteria. The pooled SVR rate after 12/24 weeks of treatment was 92.07% (95% CI: 90.39-93.61%) for the evaluated regimens. Also, the SVR rate was 91.17% (95% CI: 89.23-92.94%) in patients treated with SOF+DCV±RBV; 95.08% (95% CI: 90.88-98.13%) in patients treated with SOF+VEL±RBV; 84.97% (95% CI: 73.32-93.91%) in patients treated with SOF+VEL+VOX; and 98.54% (95% CI: 96.40-99.82%) in patients treated with GLE+PIB. The pooled SVR rate of the four regimens was 95.24% (95% CI: 93.50-96.75%) in non-cirrhotic patients and 89.39% (95% CI: 86.07-92.33%) in cirrhotic patients. The pooled SVR rate was 94.41% (95% CI: 92.02-96.42%) in treatment-naive patients and 87.98% (95% CI: 84.31-91.25%) in treatment-experienced patients. The SVR rate of GLE+PIB was higher than other regimens. SOF+VEL+VOX can be used as a treatment regimen following DAA treatment failure.
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http://dx.doi.org/10.1016/j.aohep.2020.09.012DOI Listing
January 2022

[email protected]/C with Controllable Pd Dispersion as a Highly Efficient Catalyst for Hydrogen Evolution from Ammonia Borane.

Nanomaterials (Basel) 2020 Sep 16;10(9). Epub 2020 Sep 16.

School of Chemistry and Material Science, College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun 113001, China.

A series of [email protected]/C with different Pd contents was prepared using the galvanic reduction method to disperse Pd on the surface of Cu nanoparticles on Cu/C. The dispersion of Pd was regulated by the Cu(I) on the surface, which was introduced by pulse oxidation. The CuO did not react during the galvanic reduction process and restricted the Pd atoms to a specific area. The pulse oxidation method was demonstrated to be an effective process to control the oxidization degree of Cu on Cu/C and then to govern the dispersion of Pd. The catalysts were characterized by transmission electron microscopy (TEM), high-resolution transmission electron microscope (HRTEM), high angular annular dark field scanning TEM (HAADF-STEM), energy-dispersive spectroscopy (EDS) mapping, X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), auger electron spectroscopy (AES), and inductively coupled plasma optical emission spectrometer (ICP-OES), which were used to catalyze the hydrogen evolution from ammonia borane. The [email protected]/C had much higher activity than the PdCu/C, which was prepared by the impregnation method. The TOF increased as the CuO in Cu/C used for the preparation of [email protected]/C increased, and the maximum TOF was 465 mol min mol at 298 K on [email protected]/C-640 (0.5 wt % of Pd, 640 mL of air was pulsed during the preparation of Cu/C-640). The activity could be maintained in five continuous processes, showing the strong stability of the catalysts.
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http://dx.doi.org/10.3390/nano10091850DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7558311PMC
September 2020

Preparation of 1-Hydroxy-2,5-hexanedione from HMF by the Combination of Commercial Pd/C and Acetic Acid.

Molecules 2020 May 27;25(11). Epub 2020 May 27.

College of Food and Drug, Luoyang Normal University, Luoyang 471934, China.

The development of a simple and durable catalytic system for the production of chemicals from a high concentration of a substrate is important for biomass conversion. In this manuscript, 5-hydroxymethylfurfural (HMF) was converted to 1-hydroxy-2,5-hexanedione (HHD) using the combination of commercial Pd/C and acetic acid (AcOH) in water. The influence of temperature, H pressure, reaction time, catalyst amount and the concentration of AcOH and HMF on this transformation was investigated. A 68% yield of HHD was able to be obtained from HMF at a 13.6 wt% aqueous solution with a 98% conversion of HMF. The resinification of intermediates on the catalyst was characterized to be the main reason for the deactivation of Pd/C. The reusability of the used Pd/C was studied to find that most of the activity could be recovered by being washed in hot tetrahydrofuran.
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http://dx.doi.org/10.3390/molecules25112475DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7321070PMC
May 2020

Bacterial Tropone Natural Products and Derivatives: Overview of their Biosynthesis, Bioactivities, Ecological Role and Biotechnological Potential.

Chembiochem 2020 09 8;21(17):2384-2407. Epub 2020 May 8.

Faculty of Biology, University of Freiburg, 79104, Freiburg, Germany.

Tropone natural products are non-benzene aromatic compounds of significant ecological and pharmaceutical interest. Herein, we highlight current knowledge on bacterial tropones and their derivatives such as tropolones, tropodithietic acid, and roseobacticides. Their unusual biosynthesis depends on a universal CoA-bound precursor featuring a seven-membered carbon ring as backbone, which is generated by a side reaction of the phenylacetic acid catabolic pathway. Enzymes encoded by separate gene clusters then further modify this key intermediate by oxidation, CoA-release, or incorporation of sulfur among other reactions. Tropones play important roles in the terrestrial and marine environment where they act as antibiotics, algaecides, or quorum sensing signals, while their bacterial producers are often involved in symbiotic interactions with plants and marine invertebrates (e. g., algae, corals, sponges, or mollusks). Because of their potent bioactivities and of slowly developing bacterial resistance, tropones and their derivatives hold great promise for biomedical or biotechnological applications, for instance as antibiotics in (shell)fish aquaculture.
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http://dx.doi.org/10.1002/cbic.201900786DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7497051PMC
September 2020

Collagen type VI α5 gene variations may predict the risk of lung cancer development in Chinese Han population.

Sci Rep 2020 03 19;10(1):5010. Epub 2020 Mar 19.

Department of Respiratory Medicine, The First Affiliated Hospital of School of Medicine of Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, China.

The abundant expression of collagen type VI α5 (COL6A5) exists in lung tissue, and its role in lung cancer is still unknown. We performed a genetic association study with an attempt to detect the relationships between single nucleotide polymorphisms (SNPs) in COL6A5 and lung cancer predisposition in Chinese Han population. We finally selected six tag-SNPs to determine their genotypes among 510 lung cancer patients and 495 healthy controls with the MassARRAY platform. The associations of SNPs and lung cancer risk were estimated by logistic regression method with adjustment for confounding factors. Two available databases were used for gene expression and prognosis analysis. COL6A5 rs13062453, rs1497305, and rs77123808 were significantly associated with the risk of lung cancer in the whole population or stratified subgroups (p < 0.05). Among them, COL6A5 rs13062453 and rs1497305 were also linked to the susceptibility of lung adenocarcinoma. Additionally, rs1497305 was found to be strongly related to the TNM staging under five genetic models (p < 0.05). Results from databases suggested the important role of COL6A5 in lung cancer development. COL6A5 polymorphisms rs13062453, rs1497305 and rs77123808 were associated with lung cancer risk in Chinese Han population. These findings first yield new insight of COL6A5 in lung cancer.
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http://dx.doi.org/10.1038/s41598-020-61614-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7081318PMC
March 2020

Association between Loss of Sleep-specific Waves and Age, Sleep Efficiency, Body Mass Index, and Apnea-Hypopnea Index in Human N3 Sleep.

Aging Dis 2020 Feb 1;11(1):73-81. Epub 2020 Feb 1.

1State Key Laboratory of Cognitive Neuroscience and Learning & IDG/McGovern Institute for Brain Research, Beijing Normal University, Beijing 100875, China.

Sleep spindles (SS) and K-complexes (KC) play important roles in human sleep. It has been reported that age, body mass index (BMI), and apnea-hypopnea index (AHI) may influence the number of SS or KC in non-rapid-eye-movement (NREM) 2 (N2) sleep. In this study, we investigated whether the loss of SS or KC is associated with the above factors in NREM 3 (N3) sleep. A total of 152 cases were enrolled from 2013 to 2017. The correlations between the number of SS or KC in N3 sleep and participants' characteristics were analyzed using Spearman rank correlation. Chi-squared test was used to assess the effects of age, sleep efficiency, and BMI on the loss of N3 sleep, N3 spindle and N3 KC. Our results showed that there were negative correlations between the number of SS in N3 sleep with age, BMI, and AHI ( < 0.001), and similar trends were found for KC as well. The loss of SS and KC in N3 sleep was related with age, BMI, and AHI ( < 0.01), as was the loss of N3 sleep ( < 0.01). However, sleep efficiency was not related with the loss of N3 sleep, SS and KC in N3 sleep ( > 0.05). The present study supports that age, BMI, and AHI are all influencing factors of SS and KC loss in human N3 sleep, but sleep efficiency was not an influencing factor in the loss of N3 sleep and the loss of SS and KC in N3 sleep.
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http://dx.doi.org/10.14336/AD.2019.0420DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6961777PMC
February 2020
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