Publications by authors named "Ying Chen"

4,755 Publications

  • Page 1 of 1

Protective effects and mechanisms of lycorine against adriamycin-induced cardiotoxicity.

Phytomedicine 2022 May 17;102:154178. Epub 2022 May 17.

Department of Cardiothoracic Surgery, Central Theater Command General Hospital of Chinese People's Liberation Army, 627 Wuluo Road, Wuhan 430070, China. Electronic address:

Background: Adriamycin (ADR), a high-efficiency, broad-spectrum anthraquinone chemotherapeutic agent, is currently used to treat various malignant tumors and can lead to cumulative, dose-dependent, and irreversible cardiotoxicity. Lycorine (LYC) is a benzyl phenethylamine alkaloid that exerts remarkable therapeutic effects on cancers and sepsis.

Purpose: However, researchers have not yet elucidated whether LYC exerts protective effects against cardiotoxicity induced by ADR and the possible molecular mechanisms.

Design: This study established ADR injury models in vitro and in vivo to explore the effects of LYC against cardiotoxicity induced by ADR. The effects of LYC on blood biochemical parameters, cardiac parameters and structure, ADR-related pathophysiological processes, and the SIRT1/PPARγ signal pathway in ADR-injured models, were analyzed using a series of experimental methods.

Results: LYC significantly improved survival rate, blood biochemical parameters (LDH, CK, and BUN), cardiac parameters (SV and CO), mitochondrial dysfunction, and ameliorated oxidative stress, apoptosis, and myocardial fibrosis in ADR-injured mice (p<0.05). Moreover, LYC obviously increased cell viability and reduced oxidative stress, apoptosis, and mitochondrial dysfunction in ADR-injured cells (p<0.05). Furthermore, this study confirmed that the protective effect of LYC on ADR-induced cardiotoxicitymight be mediated by the SIRT1/PPARγ signaling pathway. These results revealed that the beneficial role of LYC on cardiotoxicity induced by ADR were mediated via regulating SIRT1/PPARγ signaling for the first time.

Conclusion: These discoveries may provide a theoretical basis for the exploitation of LYC as a potential cardioprotective drug candidate due to its multiple biological functions to reduce ADR-induced cardiotoxicity, but further preclinical and clinical studies are still needed.
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http://dx.doi.org/10.1016/j.phymed.2022.154178DOI Listing
May 2022

Characteristics and feasibility of olive oil-based diacylglycerol plastic fat for use in compound chocolate.

Food Chem 2022 May 20;391:133254. Epub 2022 May 20.

Department of Food Science and Engineering, School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China; Guangdong Youmei Institute of Intelligent Bio-manufacturing, Foshan 528225, China. Electronic address:

With an aim to prepare the healthier functional chocolate, olive oil-based diacylglycerol plastic fat (ODAGP) was prepared by mixing olive oil-based diacylglycerol stearin (O-DAGS) and olive oil-based diacylglycerol olein (O-DAGO) as confectionery fat in compound chocolate. We reported the physicochemical properties of ODAGP and ODAGP-CB blends, and then evaluated their application potential in compound chocolate based on sensory, blooming property and polymorphic transition. ODAGP (40% O-DAGS) showed a wide plastic range (7.1-45.2%) and high component compatibility. The crystal properties results indicated that ODAGP mainly existed in stable β-forms (β and β). On the other hand, the ODAGP-CB binary system containing 10-40% ODAGP displayed similar melt-in-mouth property and crystal polymorphism (Form V) to natural CB. Compound chocolate prepared with ODAGP-CB blends obtained satisfactory overall acceptability (score > 7.8) and showed stronger fat bloom resistance.
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http://dx.doi.org/10.1016/j.foodchem.2022.133254DOI Listing
May 2022

PSC subtyping based on TTF-1 and p40 expression reveals distinct molecular characteristics and therapeutic strategies.

Int J Cancer 2022 May 25. Epub 2022 May 25.

Department of Thoracic Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Pulmonary sarcomatoid carcinoma (PSC) is a unique form of poorly differentiated non-small cell lung cancer (NSCLC) and is notorious for its highly malignant nature and dismal prognosis. To introduce effective treatment for PSC patients, precise subtyping of PSC is demanding. In our study, TTF-1 and P40 immunohistochemistry (IHC) staining were applied to 56 PSC patients with multi-omics data. According to IHC results, we categorized these patients into three subgroups and profiled their molecular contexture using bioinformatic skills. IHC results classified these patients into three subgroups: TTF-1 positive subgroup (n=27), P40 positive subgroup (n=15), and double-negative subgroup (n=14). Spindle cell samples accounted for 35.71% (5/14) of double-negative patients, higher than others (p=0.034). The three subgroups were heterogeneous in the genomic alteration spectrum, showing significant differences in the RTK/RAS pathway (p=0.004) and the cell cycle pathway (p=0.030). The methylation profile of the double-negative subgroup was between the other two subgroups. In similarity analysis, the TTF-1 and p40 subgroups were closely related to LUAD and LUSC, respectively. The TTF-1 positive subgroup had the highest leukocyte fraction (LF) among several cancer types, and the tumor mutation burden (TMB) of the p40 positive subgroup ranked third in the TMB list, suggesting the applicability of immunotherapy for PSC. The study established a new subtyping method of PSC based on IHC results and reveals 3 subgroups with distinct molecular features, providing evidence for refined stratification in the treatment of PSC. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/ijc.34137DOI Listing
May 2022

Encouraging specific biomarkers-based therapeutic strategies for hepatocellular carcinoma.

World J Clin Cases 2022 Apr;10(11):3321-3333

Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China.

The prevention, early discovery and effective treatment of patients with hepatocellular carcinoma (HCC) remain a global medical challenge. At present, HCC is still mainly treated by surgery, supplemented by vascular embolization, radio frequency, radiotherapy, chemotherapy and biotherapy. The application of multikinase inhibitor sorafenib, chimeric antigen receptor T cells, or PD-1/PD-L1 inhibitors can prolong the median survival of HCC patients. However, the treatment efficacy is still unsatisfactory due to HCC metastasis and postoperative recurrence. During the process of hepatocyte malignant transformation, HCC tissues can express and secrete many types of specific biomarkers, or oncogenic antigen molecules into blood, for example, alpha-fetoprotein, glypican-3, Wnt3a (one of the key signaling molecules in the Wnt/β-catenin pathway), insulin-like growth factor (IGF)-II or IGF-I receptor, vascular endothelial growth factor, secretory clusterin and so on. In addition, combining immunotherapy with non-coding RNAs might improve anti-cancer efficacy. These biomarkers not only contribute to HCC diagnosis or prognosis, but may also become molecular targets for HCC therapy under developing or clinical trials. This article reviews the progress in emerging biomarkers in basic research or clinical trials for HCC immunotherapy.
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http://dx.doi.org/10.12998/wjcc.v10.i11.3321DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9048543PMC
April 2022

Dopamine-Based Copper-Free Click Kit for Efficient Surface Functionalization.

ACS Macro Lett 2014 Oct 8;3(10):1084-1087. Epub 2014 Oct 8.

Department of Polymer Science and Department of Polymer Engineering, The University of Akron, Akron, Ohio 44325, United States.

Strain-promoted azide-alkyne cycloaddition reactions are combined with a dopamine functional species to generate a highly efficient method for surface modification. The resulting conjugate containing 4-dibenzocyclooctynol (DIBO) and dopamine results in a versatile surface labeling technology that can replicate patterns generated from photolithography and microcontact printing techniques.
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http://dx.doi.org/10.1021/mz5005162DOI Listing
October 2014

Evolving outcomes of extracorporeal membrane oxygenation during the first 2 years of the COVID-19 pandemic: a systematic review and meta-analysis.

Crit Care 2022 May 23;26(1):147. Epub 2022 May 23.

Department of Medicine, Columbia University College of Physicians and Surgeons, New York, NY, USA.

Background: Extracorporeal membrane oxygenation (ECMO) has been used extensively for coronavirus disease 2019 (COVID-19)-related acute respiratory distress syndrome (ARDS). Reports early in the pandemic suggested that mortality in patients with COVID-19 receiving ECMO was comparable to non-COVID-19-related ARDS. However, subsequent reports suggested that mortality appeared to be increasing over time. Therefore, we conducted an updated systematic review and meta-analysis, to characterise changes in mortality over time and elucidate risk factors for poor outcomes.

Methods: We conducted a meta-analysis (CRD42021271202), searching MEDLINE, Embase, Cochrane, and Scopus databases, from 1 December 2019 to 26 January 2022, for studies reporting on mortality among adults with COVID-19 receiving ECMO. We also captured hospital and intensive care unit lengths of stay, duration of mechanical ventilation and ECMO, as well as complications of ECMO. We conducted random-effects meta-analyses, assessed risk of bias of included studies using the Joanna Briggs Institute checklist and evaluated certainty of pooled estimates using GRADE methodology.

Results: Of 4522 citations, we included 52 studies comprising 18,211 patients in the meta-analysis. The pooled mortality rate among patients with COVID-19 requiring ECMO was 48.8% (95% confidence interval 44.8-52.9%, high certainty). Mortality was higher among studies which enrolled patients later in the pandemic as opposed to earlier (1st half 2020: 41.2%, 2nd half 2020: 46.4%, 1st half 2021: 62.0%, 2nd half 2021: 46.5%, interaction p value = 0.0014). Predictors of increased mortality included age, the time of final patient enrolment from 1 January 2020, and the proportion of patients receiving corticosteroids, and reduced duration of ECMO run.

Conclusions: The mortality rate for patients receiving ECMO for COVID-19-related ARDS has increased as the pandemic has progressed. The reasons for this are likely multifactorial; however, as outcomes for these patients evolve, the decision to initiate ECMO should include the best contextual estimate of mortality at the time of ECMO initiation.
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http://dx.doi.org/10.1186/s13054-022-04011-2DOI Listing
May 2022

Discrepancy Between Forceps Biopsy and Resection in Colorectal Polyps: A 1686 Paired Screening-Therapeutic Colonoscopic Finding.

Ther Clin Risk Manag 2022 16;18:561-569. Epub 2022 May 16.

Department of Gastroenterology, Tongji Hospital, Tongji University School of Medicine, Shanghai, People's Republic of China.

Purpose: To identify pathology discrepancy between forceps biopsies and polypectomy specimens in colorectal polyps, as well as the reliability of biopsy-based treatment strategy.

Methods: All endoscopic polypectomy cases with forceps biopsies performed within 6 months were included in the study. The biopsies were compared with polypectomy specimens in terms of concordance of histological diagnosis. A logistic regression model was used to investigate the independent predictors of upgrade in histological diagnosis compared with concordance in histological diagnosis.

Results: A total of 1686 paired screening-therapeutic colonoscopies and 1739 paired biopsy-polypectomy specimens were enrolled in the study. The grade of dysplasia in 84.5% of biopsy specimens were concordant to polypectomy specimens, but this proportion decreased to 75.4% when the specimens were classified using tubular or villousness structure. 10.1% and 5.4% of biopsy specimens were upgraded and downgraded in assessing grade of dysplasia, respectively, while 14.3% and 10.3% of biopsy specimens were upgraded and downgraded in assessing tubular or villousness structure, respectively. In subgroup analysis stratified by size of polyps, 9.0% and 10.6% of biopsies obtained from polyps smaller than 10 mm were upgraded in assessing dysplasia and tubular or villousness structure, respectively. This proportion increased to 10.7% and 21.3%, respectively, in biopsies obtained from polyps larger than 10 mm. Larger size of polyps and pedunculated polyps were associated with a higher incidence of upgrade in histological diagnosis. Nearly 25% of biopsy specimens with high-grade dysplasia were identified as adenocarcinoma in polypectomy specimens.

Conclusion: The concordance between biopsy and polypectomy specimens is not adequate. The biopsy-based treatment strategy is not reliable and should not be considered as an indicator for further treatment, particularly in large or pedunculated polyps.
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http://dx.doi.org/10.2147/TCRM.S358708DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9121885PMC
May 2022

Analysis of the Expression and Function of Key Genes in Pepper Under Low-Temperature Stress.

Front Plant Sci 2022 4;13:852511. Epub 2022 May 4.

College of Horticulture, Hunan Agricultural University, Changsha, China.

The mechanism of resistance of plants to cold temperatures is very complicated, and the molecular mechanism and related gene network in pepper are largely unknown. Here, during cold treatment, we used cluster analysis (-means) to classify all expressed genes into 15 clusters, 3,680 and 2,405 differentially expressed genes (DEGs) were observed in the leaf and root, respectively. The DEGs associated with certain important basic metabolic processes, oxidoreductase activity, and overall membrane compositions were most significantly enriched. In addition, based on the homologous sequence alignment of genes, we identified 14 positive and negative regulators of the ICE-CBF-COR module in pepper, including and , and compared their levels in different data sets. The correlation matrix constructed based on the expression patterns of whole pepper genes in leaves and roots after exposure to cold stress showed the correlation between 14 ICE-CBF-COR signaling module genes, and provided insight into the relationship between these genes in pepper. These findings not only provide valuable resources for research on cold tolerance, but also lay the foundation for the genetic modification of cold stress regulators, which would help us achieve improved crop tolerance. To our knowledge, this is the first study to demonstrate the relationship between positive and negative regulators related to the ICE-CBF-COR module, which is of great significance to the study of low-temperature adaptive mechanisms in plants.
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http://dx.doi.org/10.3389/fpls.2022.852511DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9116226PMC
May 2022

Biomechanical Evaluation of Seven Fixation Methods for Sagittal Split Ramus Osteotomy with Four Advancement Levels by Finite Element Analysis.

Front Surg 2022 4;9:891747. Epub 2022 May 4.

Department of Craniomaxillofacial Surgery, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: Mandibular sagittal split ramus osteotomy (SSRO) is a routine surgery to correct mandibular deformities, such as mandibular retrusion, protrusion, deficiency, and asymmetry. However, nonunion/malunion of the fragments and relapse caused by fixation failure after SSRO are major concerns. Rigid fixation to maintain postosteotomy segmental stabilization is critical for success. Additionally, understanding the biomechanical characteristics of different fixation methods in SSRO with large advancements is extremely important for clinical guidance. Therefore, the aim of the present study was to evaluate the biomechanical characteristics of different SSRO methods by finite element analysis.

Methods: SSRO finite element models with 5-, 10-, 15-, and 20-mm advancements were developed. Seven fixation methods, namely, two types of bicortical screws, single miniplate, dual miniplates, grid plate, dual L-shaped plates, and hybrid fixation, were positioned into the SSRO models. Molar and incisal biomechanical loads were applied to all models to simulate bite forces. We then investigated the immediate postoperative stability from four aspects, namely, the stability of the distal osteotomy segment, osteotomy regional stability, stress distribution on the mandible, and implant stress performance.

Results: The stability of the distal osteotomy segment and osteotomy region decreased when the advancement increased. All seven fixation methods displayed favorable biomechanical stability under minor advancement (5 mm). With large advancements, bicortical screws, dual miniplates, and grid plates provided better stability. The von Mises stress was concentrated around the screws close to the osteotomy region for the proximal segment for all fixation methods, and the von Mises stress on implants increased with larger advancements. With small advancements, five fixation methods endured tolerable maximum stresses of <880 MPa. A single miniplate and dual L-shaped plates generally suffered high stresses using larger advancements. The biomechanical characteristics were similar under molar and incisal loads.

Conclusions: The current study investigated the biomechanical properties of seven fixation devices after SSRO under molar and incisal loads. Generally, bicortical screws, grid plates, and dual miniplates provided better biomechanical stability using finite element analysis.
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http://dx.doi.org/10.3389/fsurg.2022.891747DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114864PMC
May 2022

Single-molecule real-time sequencing reveals differences in bacterial diversity in raw milk in different regions and seasons in China.

J Dairy Sci 2022 May 19. Epub 2022 May 19.

Chinese Academy of Inspection and Quarantine, Beijing 100176, People's Republic of China. Electronic address:

The quality of raw milk is a key factor influencing the whole dairy processing chain. The richness and diversity of bacteria in raw milk affect its quality and safety. However, traditional microbial detection methods mainly depend on the known microbe culture and are often time consuming. Thus, the development of efficient ways for supervising any possible microbiological contamination is desiderated. In the current work, single-molecule real-time (SMRT) sequencing, developed by Pacific Biosciences (PacBio), was applied to acquire long reads and applied for discrimination of bacteria at species level. Forty samples of raw milk obtained from Beijing, Hebei, Inner Mongolia, Shanghai, and Guangdong in China during summer, autumn, and winter were investigated. Among 35 bacteria species identified in these samples, Acinetobacter albensis, Pseudomonas gessardii, Pseudomonas weihenstephanensis, and Rahnella inusitata were the bacteria with the highest relative abundance in the overall sample, whereas the bacteria with the highest relative abundance in raw milk samples of different origins and seasons are different. Significant differences in bacterial richness and bacterial community diversity in raw milk grouped according to different production areas and different sampling seasons were confirmed by Welch's t-test. Interestingly, the transport distance and transport time positively correlated with the relative abundance of Pseudomonas weihenstephanensis, suggesting that the content of this bacteria was expected to be a standard for evaluating the freshness of raw milk. Pathogens Bacillus cereus and Klebsiella pneumoniae were detected in most samples, indicating that the raw milk was at risk of contamination by pathogenic bacteria. Moreover, the findings of this study provide important evidence for quality and safety monitoring and biological control of raw milk.
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http://dx.doi.org/10.3168/jds.2021-21445DOI Listing
May 2022

Bronchial tuberculosis: A case report.

Asian J Surg 2022 May 18. Epub 2022 May 18.

Department of Respiratory Disease, Shandong Public Health Clinical Center, Jinan, 250013, China. Electronic address:

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http://dx.doi.org/10.1016/j.asjsur.2022.04.116DOI Listing
May 2022

Mogroside V ameliorates the oxidative stress-induced meiotic defects in porcine oocytes in vitro.

Reprod Toxicol 2022 May 18. Epub 2022 May 18.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, 530004, Guangxi, China. Electronic address:

It has been reported that environmental factors, such as industrial pollution, environmental toxins, environmental hormones, and global warming contribute to the oxidative stress-induced deterioration of oocyte quality and female fertility. However, the prevention or improvement approaches have not been fully elucidated. Here, we explored the mechanism regarding how Mogroside V (MV), a main extract of Siraitia grosvenorii, improves the oxidative stress-induced meiotic defects in porcine oocytes. Our results showed that MV supplementation restores the defective oocyte maturation and cumulus cell expansion caused by HO treatment. We further found that MV supplementation promoted the oocyte cytoplasmic maturation through preventing cortical granules from the aberrant distribution, and drove the nuclear maturation by maintaining the cytoskeleton structure. Notably, our single-cell RNA sequencing data indicated that HO-treated oocytes led to the oxidative stress primarily through two pathways 'meiosis' and 'oxidative phosphorylation'. Lastly, we evaluated the effects of MV supplementation on the mitochondrial distribution pattern and membrane potential in HO-treated oocytes, revealing that MV supplementation eliminated the excessive ROS induced by the mitochondrial abnormalities and consequently suppressed the apoptosis. In conclusion, our study demonstrates that MV supplementation is an effective approach to ameliorate the oxidative stress-induced meiotic defects via recovering the mitochondrial integrity in porcine oocytes.
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http://dx.doi.org/10.1016/j.reprotox.2022.05.008DOI Listing
May 2022

TRPV1 in male reproductive system: focus on sperm function.

Mol Cell Biochem 2022 May 20. Epub 2022 May 20.

Institute of Life Science and School of Life Science, Nanchang University, No. 999 Xuefu Avenue, Honggutan District, Nanchang, 330031, Jiangxi, People's Republic of China.

The transient receptor potential vanilloid 1 (TRPV1) is a receptor used to perceive external noxious stimuli and participates in the regulation of various pathophysiological mechanisms in vivo by integrating multiple signals. The explosive growth in knowledge of TRPV1 stemmed from research on neuronal pain and heat sensation over the last decades and is being expanded tremendously in peripheral tissue research. The discovery that TRPV1 is functionally active in male animal and human reproductive tissues have attracted increasing attention in recent years. Indeed, many studies have indicated that TRPV1 is an endocannabinoid receptor that mediates Anandamide's regulation of sperm function. Other characteristics of the TRPV1 channel itself, such as calcium penetration and temperature sensitivity, have also been investigated, especially the possibility that TRPV1 could act as a mediator for sperm thermotaxis. In addition, some reproductive diseases appear to be related to the protective effects of TRPV1 on oxidative stress and heat stress. A better understanding of TRPV1 in these areas should provide strategies for tackling male infertility. This paper is the first to review the expression and mechanism of TRPV1 in the male reproductive system from molecular and cellular perspectives. A focus is given on sperm function, including calcium homeostasis, crosstalk with endocannabinoid system, participation in cholesterol-related sperm maturation, and thermotaxis, hoping to capture the current situation of this rapidly developing field.
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http://dx.doi.org/10.1007/s11010-022-04469-2DOI Listing
May 2022

Is there an optimal timing interval between hCG trigger and oocyte vitrification?

Taiwan J Obstet Gynecol 2022 May;61(3):485-488

Department of Obstetrics and Gynecology, MacKay Memorial Hospital, Taipei, Taiwan.

Objective: Oocyte vitrification has been developed as a promising alternative to slow freezing; however, the clinical outcome is highly operator dependent. From the past study, we know the timing of cryoprotectant exposure and understand that the intervals between the application of liquid nitrogen and thawing solution are crucial factors in the vitrification process. However, the optimal time intervals between hCG trigger and oocyte vitrification and equilibration remain unknown. This study aimed to evaluate the optimal intervals before and during modified vitrification.

Materials And Methods: This retrospective study included 66 patients undergoing vitrified-thawed oocyte cycles from June 2018 to May 2019. Oocyte in vitro maturation (IVM) is defined as the maturation in vitro of an immature oocyte collected using a standard pick up procedure. Oocytes were grouped into the following intervals: (1) human chorionic gonadotropin (hCG) trigger to oocyte vitrification (<38 h; 38-39 h; >39 h; IVM) (2) oocyte equilibration time (<10 min; 10-12 min; 12-15 min). The vitrification and warming procedures were performed following the steps as shown in the Cryotec method.

Results: A total of 390 mature oocytes were vitrified with the Cryotec method. The survival rates were not significantly different among the various intervals after the hCG trigger (97.59%; 95.54%; 100%); however, there was a trend of decreased survival rate in IVM group (66.67%). The oocyte survival rates were not significantly different among the various times of oocyte equilibration (96.77%; 97.33%; 95.42%).

Conclusions: This was the first study to demonstrate no correlation between oocyte survival rate and the time intervals between hCG trigger and oocyte vitrification. Nor did the oocyte survival rate correlate with the various equilibration times during vitrification, as long as the oocyte was mature. In the future, large, prospective, randomized controlled studies will be required to confirm the clinical outcomes.
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http://dx.doi.org/10.1016/j.tjog.2022.03.015DOI Listing
May 2022

Maternal diets have effects on intestinal mucosal flora and susceptibility to colitis of offspring mice during early life.

Nutrition 2022 Apr 6;99-100:111672. Epub 2022 Apr 6.

Fourth Department of the Digestive Disease Center, Suining Central Hospital, People's Republic of China. Electronic address:

Objectives: Intestinal flora is considered closely related to the occurrence of inflammatory bowel disease (IBD). This study aimed to discover whether diverse diet conditions during early life lead to different intestinal flora structure and impact different susceptibility to IBD.

Methods: We performed a randomized, controlled trial to investigate the relationship between maternal diet, intestinal flora, and susceptibility of IBD in offspring mice. We treated the maternal mice with different dietary conditions (maternal high fat, high protein, or normal diet, and offspring continued maternal diets or changed to normal diet), and then extracted bacterial meta-genomic DNA from the intestinal mucosa of the offspring during the early life and adult stages. We amplified and sequenced the conserved gene v3-v4 of the bacterial 16 S ribosomal RNA. After dextran sulphate sodium intervention, we evaluated the susceptibility to intestinal inflammation with hematoxylin and eosin stains and disease activity index scores.

Results: The number of species and alpha diversity of weaning mice (3 wk old) fed a maternal high-protein diet were significantly lower than those of the control diet group (P < 0.05). Among adult (8 wk old) offspring rats, the alpha diversity of mice that continued on a high-protein diet remained significantly decreased (P < 0.05). In addition, 12 kinds of weak bacteria were found in weaning mice fed a maternal high-protein diet compared with the control group. Offspring that continued in the maternal high-protein group had increased disease activity index and pathologic scores after weaning.

Conclusions: In general, our study shows that a maternal high-protein diet during early life can negatively regulate the intestinal flora diversity of offspring mice. A high-protein diet during early life led to higher susceptibility of IBD in offspring rats.
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http://dx.doi.org/10.1016/j.nut.2022.111672DOI Listing
April 2022

DO-Conv: Depthwise Over-Parameterized Convolutional Layer.

IEEE Trans Image Process 2022 26;31:3726-3736. Epub 2022 May 26.

Convolutional layers are the core building blocks of Convolutional Neural Networks (CNNs). In this paper, we propose to augment a convolutional layer with an additional depthwise convolution, where each input channel is convolved with a different 2D kernel. The composition of the two convolutions constitutes an over-parameterization, since it adds learnable parameters, while the resulting linear operation can be expressed by a single convolution layer. We refer to this depthwise over-parameterized convolutional layer as DO-Conv, which is a novel way of over-parameterization. We show with extensive experiments that the mere replacement of conventional convolutional layers with DO-Conv layers boosts the performance of CNNs on many classical vision tasks, such as image classification, detection, and segmentation. Moreover, in the inference phase, the depthwise convolution is folded into the conventional convolution, reducing the computation to be exactly equivalent to that of a convolutional layer without over-parameterization. As DO-Conv introduces performance gains without incurring any computational complexity increase for inference, we advocate it as an alternative to the conventional convolutional layer. We open sourced an implementation of DO-Conv in Tensorflow, PyTorch and GluonCV at https://github.com/yangyanli/DO-Conv.
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http://dx.doi.org/10.1109/TIP.2022.3175432DOI Listing
May 2022

Serotonin-norepinephrine reuptake inhibitor antidepressant effects on regional connectivity of the thalamus in persistent depressive disorder: evidence from two randomized, double-blind, placebo-controlled clinical trials.

Brain Commun 2022 15;4(3):fcac100. Epub 2022 Apr 15.

Department of Depression Evaluation Service, New York State Psychiatric Institute, 1051 Riverside Drive, Unit #51, New York, NY 10032, USA.

Previous neuroimaging studies have shown that serotonin-norepinephrine reuptake inhibitor antidepressants alter functional activity in large expanses of brain regions. However, it is not clear how these regions are systemically organized on a connectome level with specific topological properties, which may be crucial to revealing neural mechanisms underlying serotonin-norepinephrine reuptake inhibitor treatment of persistent depressive disorder. To investigate the effect of serotonin-norepinephrine reuptake inhibitor antidepressants on brain functional connectome reconfiguration in persistent depressive disorder and whether this reconfiguration promotes the improvement of clinical symptoms, we combined resting-state functional magnetic resonance imaging (fMRI) scans acquired in two randomized, double-blind, placebo-controlled trial studies of serotonin-norepinephrine reuptake inhibitor antidepressant treatment of patients with persistent depressive disorder. One was a randomized, double-blind, placebo-controlled trial of 10-week duloxetine medication treatment, which included 17 patients in duloxetine group and 17 patients in placebo group (ClinicalTrials.gov Identifier: NCT00360724); the other one was a randomized, double-blind, placebo-controlled trial of 12-week desvenlafaxine medication treatment, which included 16 patients in desvenlafaxine group and 15 patients in placebo group (ClinicalTrials.gov Identifier: NCT01537068). The 24-item Hamilton Depression Rating Scale was used to measure clinical symptoms, and graph theory was employed to examine serotonin-norepinephrine reuptake inhibitor antidepressant treatment effects on the topological properties of whole-brain functional connectome of patients with persistent depressive disorder. We adopted a hierarchical strategy to examine the topological property changes caused by serotonin-norepinephrine reuptake inhibitor antidepressant treatment, calculated their small-worldness, global integration, local segregation and nodal clustering coefficient in turn. Linear regression analysis was used to test associations of treatment, graph properties changes and clinical symptom response. Symptom scores were more significantly reduced after antidepressant than placebo administration ( = 0.18). There was a treatment-by-time effect that optimized the functional connectome in a small-world manner, with increased global integration and increased nodal clustering coefficient in the bilateral thalamus (left thalamus = 0.21; right thalamus = 0.23). The nodal clustering coefficient increment of the right thalamus (ratio = 29.86; 95% confidence interval, -4.007 to -0.207) partially mediated the relationship between treatment and symptom improvement, and symptom improvement partially mediated (ratio = 21.21; 95% confidence interval, 0.0243-0.444) the relationship between treatment and nodal clustering coefficient increments of the right thalamus. Our study may indicate a putative mutually reinforcing association between nodal clustering coefficient increment of the right thalamus and symptom improvement from serotonin-norepinephrine reuptake inhibitor antidepressant treatments with duloxetine or desvenlafaxine.
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http://dx.doi.org/10.1093/braincomms/fcac100DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113244PMC
April 2022

RNA Binding Motif Protein 3 Promotes Cell Metastasis and Epithelial-Mesenchymal Transition Through STAT3 Signaling Pathway in Hepatocellular Carcinoma.

J Hepatocell Carcinoma 2022 9;9:405-422. Epub 2022 May 9.

Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, 400044, People's Republic of China.

Purpose: RNA binding motif protein 3 (RBM3) has been reported to be dysregulated in various cancers and associated with tumor aggressiveness. Epithelial-mesenchymal transition (EMT) is an important biological process by which tumor cells acquire metastatic abilities. This study aimed to explore the regulatory and molecular mechanisms of RBM3 in EMT process.

Methods: Western blotting, IHC, and qRT-PCR were performed to evaluate the expression of target genes. Transwell assay was used to investigate the migration and invasion. RNA immunoprecipitation and luciferase reporter assay were performed to explore the correlation of RBM3 with STAT3 or microRNA-383. Animal HCC models were used to explore the role of RBM3 in metastasis in vivo.

Results: RBM3 was highly expressed in HCC tissues compared to healthy tissues, and its level was negatively correlated with the prognosis of HCC patients. RBM3 overexpression accelerated migration and invasion, promoted EMT process, and activated STAT3 signaling. EMT induced by RBM3 was not only attenuated by inhibiting pSTAT3 via S3I-201 but also abolished by suppressing STAT3 expression via siRNAs. Mechanistically, RBM3 increased STAT3 expression by stabilizing STAT3 mRNA via binding to its mRNA. As an upstream target of RBM3, microRNA-383 inhibited RBM3 expression by binding to its 3'UTR and resulted in the inhibition of the EMT process. Inhibition of RBM3 in HCC animal models prolonged survival and ameliorated malignant phenotypes in mice.

Conclusion: Our findings support that RBM3 promotes HCC metastasis by activating STAT3 signaling.
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http://dx.doi.org/10.2147/JHC.S351886DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9112182PMC
May 2022

Compatibility and Stability of Ten Commonly Used Clinical Drugs in Pediatric Electrolyte Supplements Injection.

Drug Des Devel Ther 2022 12;16:1433-1440. Epub 2022 May 12.

Department of Pharmacy, West China Second University Hospital of Sichuan University, Chengdu, People's Republic of China.

Objective: Pediatric electrolyte supplements injection is mainly used to supplement heat and body fluid, and commonly used in pediatrics. Its compatibility and stability with common clinical drugs such as antibiotics was rarely reported to ensure the children's safety and the rational use of drugs. The aim of the present study was to investigate physical and chemical stability of pediatric electrolyte supplements injection mixed with ten commonly used clinical drugs.

Methods: According to clinical drug concentration, we mix the pediatric electrolyte supplements injection mixed with ten drugs. The compatible solutions were withdrawn at certain time intervals (0, 0.5, 1, 2, 4, 6 hours) after mixing and tested by description, insoluble particles detection, pH determination and high performance liquid chromatography (HPLC) assay of active ingredient as measures of physicochemical compatibility.

Results: No obvious appearance changes were observed when mixing. Furthermore, over the 6 hours post-preparation period the pH values were within the requirements of each drug quality standard and the number of insoluble particles (≥10 and ≥25μm) met requirements of Chinese Pharmacopeia (Edition 2020) except for mezlocillin sodium for injection. The percentages of the initial concentrations maintained at a minimum of 97% in the mixtures within 6 hours.

Conclusions: Nine commonly used clinical drugs remained stable in the pediatric electrolyte supplements injection for 6 hours at 25°C and avoiding from light. Mezlocillin sodium for injection was not recommended to be combined with electrolyte supplement injection for children because its insoluble particles exceed the standard.
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http://dx.doi.org/10.2147/DDDT.S363634DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9112340PMC
May 2022

A Prediction Model for Heavy Metal Contents in Soil Based on Deep Belief Network and Tree-Based Model.

Appl Spectrosc 2022 May 18:37028221104823. Epub 2022 May 18.

530247Yanshan University School of Electrical Engineering.

In order to extract useful information from XRF (X-ray fluorescence) spectrum, and establish a high-accuracy prediction model of soil heavy metal contents, a hybrid model combined Deep Belief Network (DBN) with tree-based model was proposed. The DBN was firstly introduced into feature extraction of XRF spectral data, which can obtain deep layer features of spectrum. Owing to the strong regression ability of tree-based model, it can offset the deficiency of DBN in prediction ability, so it was used for predicting heavy metal contents based on the extracted features. In order to further improve the performance of the model, the parameters of model can be optimized according to the prediction error, which was completed by sparrow search algorithm (SSA) and the gird search. The hybrid model was applied to predict the contents of As and Pb based on spectral data of overlapping peaks. It can be obtained that R2 of As and Pb reached 0.9884 and 0.9358, the MSE of As and Pb are as low as 0.0011 and 0.0058, which outperform other commonly used models. That proved the combination of DBN and tree-based model can obtain more accurate prediction results.
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http://dx.doi.org/10.1177/00037028221104823DOI Listing
May 2022

ERBB3 methylation and immune infiltration in tumor microenvironment of cervical cancer.

Sci Rep 2022 May 17;12(1):8112. Epub 2022 May 17.

Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Soochow University, Sanxiang Road 1055, Suzhou, 215000, Jiangsu, China.

ERBB3, a member of the ERBB family of receptor tyrosine kinases, plays an important role in cancer, despite its lack of intrinsic carcinogenic mechanism of cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC). Research on bioinformatics methods through multi-omics, this work proves that ERBB3 gene mutation, methylation modification have extensive regulatory mechanisms on the CESC microenvironment. We found that ERBB3 is involved in carcinogenesis of cervical cancer and is not associated with its prognosis. The carcinogenic mechanism is mainly related to the suppression of the immune system between tumor infiltrating lymphocytes (TILs) and the methylation of the RNA level. Our study indicated ERBB3 is more likely to be a carcinogenic factor than a key prognostic factor for cervical cancer. Methylation of ERBB3 may work as a checkpoint immunotherapy target in CESC, DNA methylation modification of the 4480 base pair downstream of ERBB3 transcription initiation site was the highest.
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http://dx.doi.org/10.1038/s41598-022-11415-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114106PMC
May 2022

Application of metabolomics analysis to aid in understanding the pathogenicity of different lineages and different serotypes of Listeria monocytogenes.

Int J Food Microbiol 2022 Apr 29;373:109694. Epub 2022 Apr 29.

College of Food Science and Engineering, Nanjing University of Finance and Economics, 210023, China; Chinese Academy of Inspection and Quarantine, No.11, Ronghua South Street, Daxing District, Beijing 100176, China. Electronic address:

Listeria monocytogenes is a foodborne pathogen with high mortality in young children, elderly persons, pregnant women, and immune-compromised individuals. Most human listeriosis cases are associated with four serotypes (1/2a, 1/2b, 1/2c and 4b) within lineages I and II. The intracellular metabolic changes in L. monocytogenes from different lineages and serotypes remain unclear. Here, a non-targeted metabolomics strategy, based on ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) coupled with chemometrics was performed to analyze the metabolic fluctuations in the lineages and serotypes of L. monocytogenes. In the aggregate, 93 L. monocytogenes isolates (two lineages and four serotypes) were investigated, and a total of 48 differential biomarker metabolites were identified. Twenty differential metabolites were observed at the lineage level, while the others were found at the serotype level. At the lineage level, significantly lower contents (fold change [FC] = 3.60) of γ-aminobutyric acid (GABA) was found in lineage II strains, as compared to lineage I strains. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways enrichment analysis showed that different substances between lineages I and II participated in multiple metabolic pathways, among which pyrimidine metabolism, purine metabolism, and aminoacyl-tRNA biosynthesis were significantly enriched. The major significantly upregulated (P < 0.05) metabolites, including uridine 5'-monophosphate, uridine, guanosine monophosphate, and guanosine, in lineage II strains affect the synthesis of DNA and RNA. At the serotype level, the upregulation of urea precursor substances produced by the purine metabolism pathway in serotype 4b strains may be one reason for the increased virulence of serotype 4b strains, compared to other serotype strains. In addition, the upregulation of pyridoxine in the vitamin B6 pathway in 4b strains was also observed. The differential metabolic pathway of 1/2a and 1/2c strains is nucleotide metabolism, which could lead to differences in the biosynthesis of DNA and RNA of the strain and further affect the growth and proliferation of strains.
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http://dx.doi.org/10.1016/j.ijfoodmicro.2022.109694DOI Listing
April 2022

Generality and diversity on the kinetics, toxicity and DFT studies of sulfate radical-induced transformation of BPA and its analogues.

Water Res 2022 Apr 30;219:118506. Epub 2022 Apr 30.

MOE Key Laboratory of Deep Earth Science and Engineering, College of Architecture and Environment, Sichuan University, Chengdu 610065, China; Yibin Industrial Technology Research Institute of Sichuan University, Yibin 644000, China. Electronic address:

The international campaign to ban bisphenol A (BPA) has resulted in increasing application of BPA substitutes. However, investigations have mainly been confined to the removal of single contaminant from the water, resulting in an inefficient burden. Furthermore, systematic study and synthetical discussion of bisphenol analogues (BPs) kinetics and transformation pathways were largely underemphasized. Chemical oxidation of BPA and four typical alternatives (i.e., bisphenol AF, bisphenol E, bisphenol F and bisphenol S) in a UV-activated persulfate system was examined in this study. The effects of persulfate (PS) dosage, pH and water matrix constituents (i.e., bicarbonate, chloride and natural organic matter) were comprehensively examined using a combination of laboratory experiments and mathematical modeling. According to our findings, the removal characteristics of different BPs employing SO•-induced removal technology, including degradation mechanisms and influencing trends by water matrix, revealed similarly. The second order-rate constants of SO• reacting with BPs served as the main variables mediating the variation in degradation kinetics. Frontier molecular orbital theory and density functional theory suggested BPs molecules possessed the same susceptible positions to free radicals. In the UV-activated PS process, transformation pathways included hydroxylation, electron-transfer, substitution, and rearrangement triggered by ortho-cleavage, with certain intermediates exhibiting higher toxicity than the parent chemicals. The findings of this study provided valuable information to estimate potential environmental risks of using BPA alternatives.
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http://dx.doi.org/10.1016/j.watres.2022.118506DOI Listing
April 2022

Research on the Impact of Home Nursing Based on Intelligent Medical Internet of Things on the Quality of Life of Patients with Hemophilia.

Comput Math Methods Med 2022 4;2022:4976303. Epub 2022 May 4.

Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.

The purpose of this paper is to study the feasibility and economic benefits of intelligent medical Internet of Things (IOT) systems for improving the quality of life of hemophilia patients, thereby reducing the risk of teratogenicity and disability for patients. This article selects 60 severe hemophilia patients who were followed up in our hospital from 2018 to 2019 as the research object. In the intelligent medical system, the Gaussian mixture model discretization algorithm is used to preprocess patient data collection. The observation group uses the intelligent medical system to implement home nursing for the patients, and the control group uses ordinary home nursing. This paper evaluates the quality of life of the two groups of patients 6 months after the intervention, including self-care ability, transfer function, and home nursing cognitive ability. The research results show that the home nursing based on smart medical IOT proposed in this paper is feasible and effective for improving the quality of life of patients. It can effectively improve the patient's self-care ability and joint functions and has important reference value for the development of intelligent medical IOT equipment.
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http://dx.doi.org/10.1155/2022/4976303DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9095391PMC
May 2022

Synthesis and Characterization of Alkyne-Functionalized Photo-Cross-Linkable Polyesters.

ACS Omega 2022 May 26;7(18):15540-15546. Epub 2022 Apr 26.

Meinig School of Biomedical Engineering, College of Engineering, Cornell University, Ithaca, New York 14853, United States.

An alkyne-functionalized elastomer derived from sebacic acid, 1,3-propanediol, and alkyne-functionalized serinol is synthesized via melt condensation. A low-power UV lamp triggers the cross-linking rapidly via thiol-yne click chemistry. The cross-linking behavior is studied by photorheology and NMR spectroscopy. The resultant elastomer possesses mechanical properties similar to those of human soft tissues and exhibits degradability and good cytocompatibility.
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http://dx.doi.org/10.1021/acsomega.2c00272DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9096926PMC
May 2022

Exploiting Wavelength Orthogonality in Photoinitiated RAFT Dispersion Polymerization and Photografting for Monodisperse Surface-Functional Polymeric Microspheres.

ACS Macro Lett 2022 May 10:716-722. Epub 2022 May 10.

Department of Polymeric Materials and Engineering, School of Materials and Energy, Guangdong University of Technology, Guangzhou 510006, China.

We report a strategy toward surface-functional polymeric microspheres using a wavelength orthogonality technique that employs photoinitiated reversible addition-fragmentation chain transfer (RAFT) dispersion polymerization and the subsequent photografting under different wavelengths of light. Initial screening of reaction conditions indicated photoreactive polymeric microsphere with uniform sizes could be prepared by using photoinitiator-functionalized macro-RAFT agents under purple light irradiation. Photoreactive polymeric microspheres allowed photografting polymerizations under UV light irradiation, and we further demonstrated the broad scope of this method by photografting acrylamide, acrylic, and methacrylic monomers. Finally, carboxyl-functionalized polymeric microspheres with an exceptional high number of carboxyl groups were successfully prepared by this technique, which permitted extensive surface bioconjugation of model proteins (e.g., streptavidin). This method should expand the capabilities of RAFT dispersion polymerization to afford diverse surface-functional polymeric microspheres for some specific applications.
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http://dx.doi.org/10.1021/acsmacrolett.2c00228DOI Listing
May 2022

Homogeneous nuclei-induced, secondary nuclei-induced, and spontaneous whey protein concentrate nanofibril formation through different pathways.

J Dairy Sci 2022 May 12. Epub 2022 May 12.

Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China. Electronic address:

The addition of homogeneous nuclei (HN) or secondary nuclei (SN) could lead to different kinetics and thermodynamics as the nucleation energy barrier decreases and the lag time is shortened to different degrees compared with spontaneous fibrillation. To explain these differences, we monitored the formation and depletion of HN during fibril formation and found that both SN-induced fibrils and HN-induced fibrils follow the same nucleated growth pathway as spontaneously formed WPC fibrils. Moreover, there were also other paths, which were confirmed by X-ray diffraction, transmission electron microscopy, and atomic force microscopy. The surfaces of the SN could recruit monomers and resulted in stronger intersheet stacking and a larger fibril height and periodicity. The HN incorporation led to a propensity for hydrogen-bonding interactions and a longer fibril. Fibrillation by the addition HN and SN followed both common and distinct pathways, as spontaneous fibrillation and led to different capacities to induce fibrillation.
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http://dx.doi.org/10.3168/jds.2021-21630DOI Listing
May 2022

Response surface model based emission source contribution and meteorological pattern analysis in ozone polluted days.

Environ Pollut 2022 May 11:119459. Epub 2022 May 11.

Guangdong Provincial Key Laboratory of Atmospheric Environment and Pollution Control, College of Environment and Energy, South China University of Technology, Guangzhou Higher Education Mega Center, Guangzhou, 510006, China.

Urban and regional ozone (O) pollution is a public health concern and causes damage to ecosystems. Due to the diverse emission sources of O precursors and the complex interactions of air dispersion and chemistry, identifying the contributing sources of O pollution requires integrated analysis to guide emission reduction plans. In this study, the meteorological characteristics leading to O polluted days (in which the maximum daily 8-h average O concentration is higher than the China Class II National O Standard (160 μg/m)) in Guangzhou (GZ, China) were analyzed based on data from 2019. The O formation regimes and source apportionments under various prevailing wind directions were evaluated using a Response Surface Modeling (RSM) approach. The results showed that O polluted days in 2019 could be classified into four types of synoptic patterns (i.e., cyclone, anticyclone, trough, and high pressure approaching to sea) and were strongly correlated with high ambient temperature, low relative humidity, low wind speed, variable prevailing wind directions. Additionally, the cyclone pattern strongly promoted O formation due to its peripheral subsidence. The O formation was nitrogen oxides (NO)-limited under the northerly wind, while volatile organic compounds (VOC)-limited under other prevailing wind directions. Anthropogenic emissions contributed largely to the O formation (54-78%) under the westerly, southwesterly, easterly, southeasterly, or southerly wind, but only moderately (35-47%) under the northerly or northeasterly wind. Furthermore, as for anthropogenic contributions, local emission contributions were the largest (39-60%) regardless of prevailing wind directions, especially the local NO contributions (19-43%); the dominant upwind regional emissions contributed 12-46% (e.g., contributions from Dongguan were 12-20% under the southeasterly wind). The emission control strategies for O polluted days should focus on local emission sources in conjunction with the emission reduction of upwind regional sources.
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http://dx.doi.org/10.1016/j.envpol.2022.119459DOI Listing
May 2022

Circ_0001273 downregulation inhibits the growth, migration and glutamine metabolism of esophageal cancer cells via targeting the miR-622/SLC1A5 signaling axis.

Thorac Cancer 2022 May 13. Epub 2022 May 13.

Department of Thoracic Surgery, Gaozhou People's Hospital, Gaozhou City, Guangdong Province, China.

Background: Esophageal cancer is a relatively rare cancer. However, its death rate is not to be taken lightly. Accumulating evidence indicates circular RNA (circRNA) is implicated in cancer development. The objective of this study was to unveil the role of circ_0001273 in esophageal cancer (EC).

Methods: For expression analysis of circ_0001273, miR-622 and solute carrier family 1 member 5 (SLC1A5), quantitative real-time PCR (qPCR) and Western blot were conducted. Cell proliferation was evaluated by cell counting kit-8 (CCK-8), EdU and colony formation assays. Cell apoptosis and cell migration were investigated using flow cytometry assay and wound healing assay. Glutamine metabolism was assessed by glutamine consumption and glutamate production using matched kits. The predicted binding relationship between miR-622 and circ_0001273 or SLC1A5 was validated by dual-luciferase reporter assay. An in vivo xenograft model was established to determine the role of circ_0001273 on tumor growth.

Results: Circ_0001273 was upregulated in EC tumor tissues and cells. Knockdown of circ_0001273 repressed EC cell proliferation, migration, epithelial-mesenchymal transition (EMT) and glutamine metabolism. Circ_0001273 knockdown also blocked tumor development in animal models. MiR-622 was targeted by circ_0001273, and its inhibition reversed the functional effects of circ_0001273 knockdown. SLC1A5 was a target gene of miR-622, and circ_0001273 targeted miR-622 to positively regulate SLC1A5 expression. The inhibitory effects of miR-622 enrichment on EC cell proliferation, migration, EMT and glutamine metabolism were recovered by SLC1A5 overexpression.

Conclusion: Circ_0001273 high expression contributed to EC progression via modulating the miR-622/SLC1A5 signaling axis.
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http://dx.doi.org/10.1111/1759-7714.14458DOI Listing
May 2022

Optimization of Fibre Extraction and Properties for Bio-Composite Applications Using Desirability Function Analysis.

Polymers (Basel) 2022 Apr 21;14(9). Epub 2022 Apr 21.

E1-349 EITC, Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB R3T 5V6, Canada.

The effect of extraction time, temperature, and alkali concentration on the physical and mechanical properties of cattail ( L.) fibres were investigated using five levels of time (4, 6, 8, 10, and 12 h), four levels of temperature (70, 80, 90, and 95 °C), and three levels of NaOH concentration (4, 7, 10%, /) in a 3 × 4 × 5 factorial experimental design. The extraction parameters were optimized for bio-composite application using a desirability function analysis (DFA), which determined that the optimum extraction time, temperature and NaOH concentration were 10 h, 90 °C, and 7%, respectively. A sensitivity analysis for optimal treatment conditions confirmed that the higher overall desirability does not necessarily mean a better solution. However, the analysis showed that the majority of optimum settings for time, temperature, and concentration of NaOH found in the sensitivity analysis matched with the optimum conditions determined by DFA, which confirmed the validity of the optimum treatment conditions.
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http://dx.doi.org/10.3390/polym14091685DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100250PMC
April 2022
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