Publications by authors named "Yinfeng Chen"

9 Publications

  • Page 1 of 1

The Cohesive Energy and Vibration Characteristics of Parallel Single-Walled Carbon Nanotubes.

Molecules 2021 Dec 10;26(24). Epub 2021 Dec 10.

School of Mechanical Engineering, Jiangnan University, Wuxi 214122, China.

Based on the van der Waals (vdW) interaction between carbon atoms, the interface cohesive energy between parallel single-walled carbon nanotubes was studied using continuous mechanics theory, and the influence of the diameter of carbon nanotubes and the distance between them on the cohesive energy was analyzed. The results show that the size has little effect on the cohesive energy between carbon nanotubes when the length of carbon nanotubes is over 10 nm. At the same time, we analyzed the cohesive energy between parallel carbon nanotubes with the molecular dynamics simulation method. The results of the two methods were compared and found to be very consistent. Based on the vdW interaction between parallel carbon nanotubes, the vibration characteristics of the two parallel carbon nanotube system were analyzed based on the continuous mechanical Euler-beam model. The effects of the vdW force between carbon nanotubes, the diameter and length of carbon nanotubes on the vibration frequency of carbon nanotubes was studied. The obtained results are helpful in improving the understanding of the vibration characteristics of carbon nanotubes and provide an important theoretical basis for their application.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/molecules26247470DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8707357PMC
December 2021

Evaluation of EpCAM-specific exosomal lncRNAs as potential diagnostic biomarkers for lung cancer using droplet digital PCR.

J Mol Med (Berl) 2022 Jan 14;100(1):87-100. Epub 2021 Oct 14.

Department of Thoracic Surgery, The Affiliated Hospital of Medical School of Ningbo University, 247 Renmin Road, Ningbo, 315020, Zhejiang, China.

Accumulating evidence demonstrated that long non-coding RNAs (lncRNAs) derived from exosomes had the potential to be diagnostic markers for lung cancer. However, the diagnostic value of lncRNAs from epithelial cell adhesion molecule (EpCAM)-positive exosomes remains unclear. In the study, serum EpCAM-positive exosomes were isolated with magnetic beads, and their role in lung cancer was investigated in vitro and in vivo. The copy numbers of lncRNAs RP11-77G23.5 and PHEX-AS1 in EpCAM-specific exosomes were quantified by droplet digital PCR (ddPCR). The diagnostic value of RP11-77G23.5 and PHEX-AS1 was tested in the training cohort and verified in the validation cohort. We found that EpCAM-specific exosomes could promote lung cancer development in vitro and in vivo. RP11-77G23.5 and PHEX-AS1 were significantly elevated in EpCAM-specific exosomes from lung cancer patients and could distinguish malignant from benign lung tumors. The amounts of RP11-77G23.5 were statistically higher in the subtype of lung adenocarcinoma (LUAC) than that of lung squamous cell carcinoma (LUSC), showing its capability to subtype LUAC and LUSC, while PHEX-AS1 exhibited distinct expression signatures between lower and higher tumor stages, and without and with distant metastasis, indicating its association with lung cancer progression. In conclusion, the EpCAM-specific exosomal lncRNAs RP11-77G23.5 and PHEX-AS1 may be promising diagnostic biomarkers for lung cancer. KEY MESSAGES: Serum EpCAM-positive exosomes promote lung cancer development in vitro and in vivo. Two EpCAM-specific exosomal lncRNAs can be simultaneously detected by RT-ddPCR. EpCAM-specific exosomal RP11-77G23.5 has the potential to subtype LUAC and LUSC. EpCAM-specific exosomal PHEX-AS1 is associated with lung cancer progression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00109-021-02145-4DOI Listing
January 2022

Gut microbiota in healthy and unhealthy long-living people.

Gene 2021 May 16;779:145510. Epub 2021 Feb 16.

Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, College of Life Science and Engineering, Foshan University, Foshan, China. Electronic address:

The human gut microbiota in long-living people has been characterized, however, its metabolic potential is still largely unknown in this group. In this study, the gut microbiota was assessed in 37 Chinese long-living participants (aged 90 + years) by metagenomic sequencing of stool samples. Participants were categorized into two groups, healthy long-living (n = 28) and unhealthy long-living (n = 9). Gut microbiota composition and function were compared among these two groups. We found that the gut microbiota in the healthy long-living group was significantly separated from the unhealthy group. The healthy long-living group contained a higher abundance of Bacteroidetes and more functional pathways in energy metabolism, glycan biosynthesis and metabolism, metabolism of cofactors and vitamins, and biosynthesis of other secondary metabolites. The unhealthy group contained a higher abundance of Streptococcus and other pathogenic bacteria, and also contained more functional pathways for xenobiotics biodegradation and metabolism than the healthy group. Additionally, the unhealthy group had decreased levels of carbohydrate-active enzymes, including host-glycan and fiber degrading enzymes, and an increase in starch-degrading enzymes. In conclusion, the gut microbiota of unhealthy long-living people contains more pathogenic bacteria, and the overall gut microbiota may be in an unhealthy state, "dysbiosis", which leads to a decrease in carbohydrate digestion, glycan and thiamine (B1) metabolites, and fatty acid biosynthesis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.gene.2021.145510DOI Listing
May 2021

The carnivorous digestive system and bamboo diet of giant pandas may shape their low gut bacterial diversity.

Conserv Physiol 2020 13;8(1):coz104. Epub 2020 Mar 13.

Department of Animal Science, Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, Sichuan, 611130, China.

The gut microbiota diversity of eight panda cubs was assessed during a dietary switch.Gut microbiota diversity of panda cubs significantly decreased after bamboo consumption.Carnivorous species living on a plant-based diet possess low microbial diversity.Mice were fed a bamboo diet but did not display low gut microbiota diversity.

Giant pandas have an exclusive diet of bamboo; however, their gut microbiotas are more similar to carnivores than herbivores in terms of bacterial composition and their functional potential. This is inconsistent with observations that typical herbivores possess highly diverse gut microbiotas. It is unclear why the gut bacterial diversity of giant pandas is so low. Herein, the dynamic variations in the gut microbiota of eight giant panda cubs were measured using 16S rRNA gene paired-end sequencing during a dietary switch. Similar data from red panda (an herbivorous carnivore) and carnivorous species were compared with that of giant pandas. In addition, mice were fed a high-bamboo diet (80% bamboo and 20% rat feed) to determine whether a bamboo diet could lower the gut bacterial diversity in a non-carnivorous digestive tract. The diversity of giant panda gut microbiotas decreased significantly after switching from milk and complementary food to bamboo diet. Carnivorous species living on a plant-based diet, including giant and red pandas, possess a lower microbial diversity than other carnivore species. Mouse gut microbiota diversity significantly increased after adding high-fibre bamboo to their diet. Findings suggest that a very restricted diet (bamboo) within a carnivorous digestive system might be critical for shaping a low gut bacterial diversity in giant pandas.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/conphys/coz104DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7066643PMC
March 2020

Transplant of microbiota from long-living people to mice reduces aging-related indices and transfers beneficial bacteria.

Aging (Albany NY) 2020 03 16;12(6):4778-4793. Epub 2020 Mar 16.

Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan, China.

A close relationship between age and gut microbiota exists in invertebrates and vertebrates, including humans. Long-living people are a model for studying healthy aging; they also have a distinctive microbiota structure. The relationship between the microbiota of long-living people and aging phenotype remains largely unknown. Herein, the feces of long-living people were transplanted into mice, which were then examined for aging-related indices and beneficial bacteria. Mice transplanted with fecal matter from long-living people (L group) had greater α diversity, more probiotic genera ( and ), and short-chain fatty acid producing genera () than the control group. L group mice also accumulated less lipofuscin and β-galactosidase and had longer intestinal villi. This study indicates the effects that the gut microbiota from long-living people have on healthy aging.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.18632/aging.102872DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7138539PMC
March 2020

Buckling behaviors of single-walled carbon nanotubes inserted with a linear carbon-atom chain.

Nanotechnology 2018 Aug 29;29(33):335704. Epub 2018 May 29.

Jiangsu Key Laboratory of Advanced Food Manufacturing Equipment and Technology, Jiangnan University, 214122 Wuxi, People's Republic of China.

Buckling behaviors of single-walled carbon nanotubes (SWCNTs) inserted with a linear carbon-atom chain (CAC) (the composite structures are also called carbon nanowires (CNWs)) under torsion and bending as well as compression are studied using molecular dynamics (MD) simulations, respectively. Our MD results show that the critical buckling angles (or strains) of CNWs under the three presented kinds of loading patterns can be two times those of corresponding independent SWCNTs for long CNWs, while the buckling improvement is not obvious for short ones. The main reason is that the radial van der Waals force between the CAC and the SWCNT is very small for a short CNW, while it increases with increasing length and then tends to a constant for a long CNW. The obtained MD results agree well with those from available theoretical models. These findings will be a great help towards understanding the stability and reliability of the special CNT structures, and designing flexible CNT-based devices.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1088/1361-6528/aac84fDOI Listing
August 2018

LncRNA MIAT: Myocardial infarction associated and more.

Gene 2016 Mar 17;578(2):158-61. Epub 2015 Dec 17.

Guang'anmen Hospital, China Academy of Chinese Medical Sciences, No.5 Beixiange Street, Xicheng District, Beijing 100053, China. Electronic address:

Non-coding RNAs are emerging as regulatory RNAs that participate in the regulation of gene expression and play vital roles in various biological and pathological processes. Long non-coding (lncRNA) is a novel class of non-coding RNAs that regulates gene expression by binding to chromatin regulators and interfering RNAs to affect cellular response. Myocardial infarction associated transcript (MIAT) is identified as lncRNAs, which is involved in various diseases, pathological and physiological processes, such as myocardial infarction, diabetic retinopathy, paranoid schizophrenia, microvascular dysfunction and formation of nuclear bodies, and neurogenic commitment. Here we review the growing understanding and potential utilization of MIAT.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.gene.2015.12.032DOI Listing
March 2016

Xuezhikang Capsule for Type 2 Diabetes with Hyperlipemia: A Systematic Review and Meta-Analysis of Randomized Clinical Trails.

Evid Based Complement Alternat Med 2015 13;2015:468520. Epub 2015 Jul 13.

Department of Cardiology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beixiange 5, Xicheng District, Beijing 100053, China.

Objective. To evaluate the efficacy and safety of Xuezhikang capsule treating type 2 diabetes with hyperlipidemia. Methods. We searched six databases to identify relevant studies published before January 2015. Two review authors independently extracted data and assessed the Cochrane risk of bias tool. We resolved disagreements with this assessment through discussion and a decision was achieved by consensus. Results. We included 21 studies (1548 participants). Treatment courses were at least 8 weeks. Overall, the risk of bias of included trials was unclear. Among them, 16 studies could conduct meta-analysis. The result showed that compared with routine group (5 studies), Xuezhikang group had more effect on decreasing TC, TG, LDL-C, and rising HDL-C. However, compared with statins group (11 studies), Xuezhikang group has less effect on decreasing TC, TG, and rising HDL-C. Meanwhile, two groups had no statistical differences of LDL-C level. Conclusion. Xuezhikang capsule may be effective for treating type 2 diabetes with hyperlipemia. Our findings should be considered cautiously due to unclear risk of bias of the included studies and low methodological quality. Therefore, more strictly designed large-scale randomized clinical trials are needed to evaluate the efficacy of Xuezhikang capsule in type 2 diabetes with hyperlipemia.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2015/468520DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4515518PMC
August 2015

An ultrasensitive peroxydisulfate electrochemiluminescence immunosensor for Streptococcus suis serotype 2 based on L-cysteine combined with mimicking bi-enzyme synergetic catalysis to in situ generate coreactant.

Biosens Bioelectron 2013 May 10;43:63-8. Epub 2012 Dec 10.

Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China.

A novel signal amplification strategy of mimicking bi-enzyme synergetic catalysis to generate coreactant in situ was designed to fabricate an ultrasensitive peroxydisulfate electrochemiluminescence (ECL) immunosensor for detection of Streptococcus suis serotype 2 (SS2). It was the first time to detect SS2 by using ECL. Through the interaction between l-cysteine (l-cys) and hollow PtPd bimetal alloy nanoparticles (HPtPd) to form ((l-cys-HPtPd)n) nanocomposites, the loading amount of l-cys and HPtPd was greatly increased, which could greatly enhance the ECL signal of peroxydisulfate. At the same time, Glucose Oxidase (GOD), used to block nonspecific binding sites of (l-cys-HPtPd)n nanocomposites, could rapidly oxidize d-glucose in the detection solution into gluconic acid accompanying with the generation of H2O2, which was further catalyzed by HPtPd to generate O2. And O2, acted as the coreactant of peroxydisulfate, could greatly amplify the ECL signal. In the process, HPtPd could be regarded as mimicking enzyme, the effect of which was similar to horseradish peroxidase (HRP) in generating O2. With the several amplification factors of a sandwich-type structure we designed, a wide linear ranged from 0.0001 to 100ngmL(-1) was acquired with a relatively low detection limit of 33fgmL(-1) for SS2. The present work demonstrated that the novel strategy had the great advantages in sensitivity, selectivity and reproducibility which might hold a new promise for highly sensitive bioassays applied in clinical detection.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bios.2012.11.038DOI Listing
May 2013
-->