Publications by authors named "Yiming Ma"

162 Publications

Photochromic and Electric Field-Regulating Luminescence in High-Transparent (K,Na)NbO-Based Ferroelectric Ceramics with Two-Phase Coexistence.

ACS Appl Mater Interfaces 2022 Aug 1;14(31):35940-35948. Epub 2022 Aug 1.

Faculty of Printing Packaging Engineering and Digital Media Technology, Xi'an University of Technology, Xi'an 710048, China.

Rare earth Tb doped (KNaLi)(NbBi)O (KNNLB-%Tb) lead-free transparent ferroelectric ceramics were designed and prepared. The effects of Tb on phase structure, microstructure, optical transmittance, photoluminescence, and photochromic behaviors were studied. Although two ferroelectric phases coexist, the optical transmittance can reach the high value of 74% in the visible light region because of the fine grains, dense ceramic microstructure, large optical energy band gap, and relatively high symmetry of coexisting ferroelectric phases. In addition, Tb works as a luminescent center, and the reversible photochromic modulation is achieved by alternate stimulation of illumination and heat treatment. Meanwhile, the luminescence contrast can be improved under electric field stimulation due to the easy change of lattice symmetry in coexisting ferroelectric phases. The generation of color centers after illumination and the local crystal field around the luminescent center caused by electric field contributes to above phenomena. These ceramics exhibit the great potential in optical data storage and anticounterfeiting.
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http://dx.doi.org/10.1021/acsami.2c09564DOI Listing
August 2022

Extracellular Vesicle Derived From Mesenchymal Stem Cells Have Bidirectional Effects on the Development of Lung Cancer.

Front Oncol 2022 4;12:914832. Epub 2022 Jul 4.

Department of Pulmonary and Critical Care Medicine, The Second Xiangya Hospital, Central South University, Changsha, China.

Mesenchymal stem cell is a kind of pluripotent cells with the ability of self-renewal and multi-directional differentiation, which exist in bone marrow, umbilical cord blood, umbilical cord tissue, placenta tissue, adipose tissue and so on. Extracellular vesicles are membranous lipid vesicles secreted by a variety of cells and widely present in body fluids, which contain proteins, mRNA, microRNA and other substances, and are an important medium of intercellular communication. At present, more and more evidence shows that mesenchymal stem cell-derived extracellular vesicles play an important role in the development of lung cancer. Regulating the levels of proteins, RNAs and other substances in MSC-EVs and then transplanting them into patients may be a new way to alleviate the development of lung cancer. We mainly introduce the role of extracellular vesicles derived from human umbilical cord mesenchymal stem cells, bone marrow mesenchymal stem cells and adipose mesenchymal stem cells in lung cancer, to provide new alternatives for the treatment of lung cancer.
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http://dx.doi.org/10.3389/fonc.2022.914832DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9289533PMC
July 2022

High-fat diet alleviates colitis by inhibiting ferroptosis via solute carrier family seven member 11.

J Nutr Biochem 2022 Jul 17;109:109106. Epub 2022 Jul 17.

Department of Gastrointestinal Surgery, Peking University Shenzhen Hospital, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center, Guangdong province, China. Electronic address:

A high-fat diet (HFD) is reported to exacerbate ulcerative colitis by inducing obesity, which conceals the effect of the diet itself. Ferroptosis, a type of regulated cell death induced by lipid hydroperoxides, has recently been reported in colitis. Here, we aimed to determine whether HFD affects ferroptosis and colitis progression in an obesity-independent manner. We subjected male C57BL/6J mice to either an HFD (60% fat diet) or isocaloric control diet (10% fat diet) for 4 weeks, followed by inducing colitis with 2.5% dextran sulfate sodium (DSS). Compared with the isocaloric control diet, non-obesogenic HFD reduced DSS-induced colonic mucosal injury, as shown by disease activity index, colon thickness, inflammatory infiltrations, and mucosal damage index; however, there were no differences in body weight, Lee's index, and omental fat weight between the two groups. HFD mice exhibited decreased lipid peroxidation and ferroptosis markers expression in colon tissues. Furthermore, a lipid mixture protected gut organoids and normal colonic epithelial cells from RSL3-induced ferroptosis. Mechanistically, the lipid mixture prevented glutathione deficiency by upregulating the cysteine transporter, solute carrier family seven member 11. Collectively, these findings suggest that an HFD ameliorates DSS-induced colitis through ferroptosis repression in an obesity-independent manner and provide new evidence to evaluate the effects of an HFD on colitis.
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http://dx.doi.org/10.1016/j.jnutbio.2022.109106DOI Listing
July 2022

Follow-Up of Surgical or Nonsurgical Patients with Pulmonary Cryptococcosis: A Real-World Study.

Infect Drug Resist 2022 11;15:3669-3681. Epub 2022 Jul 11.

Department of Respiratory and Critical Care Medicine, the Second Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.

Background: Surgical and medical treatments are applied to pulmonary cryptococcosis (PC) in the real world, while the prognosis of different therapies is uncertain. This study investigated diagnosis, real-world therapy, follow-up outcomes, and prognosis factors, aiming to deepen our understanding of PC.

Methods: Patients pathologically diagnosed with PC were retrospectively reviewed and followed up. Further comparisons and subgroup analyses were conducted in surgical and nonsurgical treatment individuals. Univariable and multivariable logistic regression methods were used to explore the risk factors associated with treatment failure.

Results: One hundred and sixty-three patients were included in this study, of whom 92 underwent surgical removal of VATS or open lung surgery (68 of them received postoperative antifungal treatment) and 71 got antifungal drugs only. Compared with nonsurgical patients, surgical patients were more immunocompetent (73 [79.3%] cases vs 33 [46.5%]), showed milder symptoms and more limited pulmonary lesions. Although they had instant treatment response owing to lesions resection, there is no significant advantage in the rate of treatment failure. Multivariable regression showed independent predictive factors associated with treatment failure were polymorphonuclear (PMN)>6.30*10/L, albumin (Alb) <40g/L and antifungal dosage <400mg/d. Further analysis among patients with different immune statuses or symptoms demonstrated that sufficient antifungal dosage could reduce the rate of treatment failure.

Conclusion: PC showed variable and nonspecific clinical features. PC patients with limited nodules/masses and mild symptoms often led to misdiagnosis and unnecessary lung resections. The potential risk factors including higher PMN and hypoalbuminemia could help clinicians to identify PC patients with poor treatment efficiency at an early stage. To note, sufficient antifungal dosage may improve the treatment outcomes.
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http://dx.doi.org/10.2147/IDR.S352966DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9285854PMC
July 2022

A pressure-resistant zwitterionic skin sensor for domestic real-time monitoring and pro-healing of pressure injury.

Biosens Bioelectron 2022 Oct 3;214:114528. Epub 2022 Jul 3.

Department of Biochemical Engineering, Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300350, China; Frontier Technology Research Institute, Tianjin University, Tianjin, 301700, China. Electronic address:

Pressure injury (PI) is a hard-to-heal wound to patients with the limited mobility, especially paralyzed or elderly persons. These patients also commonly suffer from sensation loss or dementia that is unable to indicate symptoms in time, resulting in missing the "golden period" for treatment. Therefore, it is highly required to domestic continously real-time monitoring as well as promoting wound healing of PI. However, no existing device has realized these functions for PI. Herein, we prepare a zwitterionic skin sensor that enables pro-healing as well as domestic real-time monitoring the multi-indicators of PI. To apply for a PI dressing that requires to tolerate patient body weight, organosilicon nanoparticles (OSNPs) are designed as crosslinks in the zwitterionic conductive hydrogel (CH-OSNP), which exhibits pressure-resistant properties (99.81% compression to recovery) as well as anti-bacterial adhesion. Moreover, the CH-OSNP-based skin sensor is developed, and the resultant sensor can be sensitive to stress stimuli even under a long-term constant heavy load, which stimulates the pressure of a PI person lying down. In vivo results show that this sensor can not only promote PI healing, but also continuously monitor and distinguish multiple information, such as exudate, swelling, and infection, to prevent PI from being worsen. This work provides a domestic feasible device to cure and monitor the PI of patients.
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http://dx.doi.org/10.1016/j.bios.2022.114528DOI Listing
October 2022

Predicting resting energy expenditure in people with chronic spinal cord injury.

Spinal Cord 2022 Jul 2. Epub 2022 Jul 2.

Faculty of Behavioural and Movement Sciences, Vrije Universiteit Amsterdam, Amsterdam Movement Sciences, Amsterdam, the Netherlands.

Study Design: Cross-sectional study.

Objectives: The aims of this study were (1) to validate the two recently developed SCI-specific REE equations; (2) to develop new prediction equations to predict REE in a general population with SCI.

Setting: University, the Netherlands.

Methods: Forty-eight community-dwelling men and women with SCI were recruited (age: 18-75 years, time since injury: ≥12 months). Body composition was measured by dual-energy X-ray absorptiometry (DXA), single-frequency bioelectrical impedance analysis (SF-BIA) and skinfold thickness. REE was measured by indirect calorimetry. Personal and lesion characteristics were collected. SCI-specific REE equations by Chun et al. [1] and by Nightingale and Gorgey [2] were validated. New equations for predicting REE were developed using multivariate regression analysis.

Results: Prediction equations by Chun et al. [1] and by Nightingale and Gorgey [2] significantly underestimated REE (Chun et al.: -11%; Nightingale and Gorgey: -11%). New equations were developed for predicting REE in the general population of people with SCI using FFM measured by SF-BIA and Goosey-Tolfrey et al. skinfold equation (R = 0.45-0.47; SEE = 200 kcal/day). The new equations showed proportional bias (p < 0.001) and wide limits of agreement (LoA, ±23%).

Conclusions: Prediction equations by Chun et al. [1] and by Nightingale and Gorgey [2] significantly underestimated REE and showed large individual variations in a general population with SCI. The newly developed REE equations showed proportional bias and a wide LoA (±23%) which limit the predictive power and accuracy to predict REE in the general population with SCI. Alternative methods for measuring REE need to be investigated.
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http://dx.doi.org/10.1038/s41393-022-00827-5DOI Listing
July 2022

A prospective study on sex differences in functional capacity, quality of life and prognosis in patients with heart failure.

Medicine (Baltimore) 2022 Jul 1;101(26):e29795. Epub 2022 Jul 1.

Cardiology Department, the First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China.

Background: Due to the lack of evidence and inconsistency of sex differences in Heart failure (HF) in the Chinese population, this study aimed to compare sex differences in functional capacity and quality of life (QoL) between women and men after standard HF medications therapies, and analyze whether sex differences were associated with the composite endpoints of all-cause mortality or HF-related hospitalization and cardiac event-free survival rate in Chinese patients with HF.

Methods: This was a 1-year longitudinal study. Participants included patients with HF from March 2017 to December 2018. At baseline and followed up at 1, 6, and 12 months later, functional capacity was assessed by 6-minute walk testing (6MWT), QoL was measured with the Kansas City Cardiomyopathy Questionnaire (KCCQ) and EuroQoL five dimensions (EQ-5D). The Cox proportional hazards model and Kaplan-Meier curves were used to determine sex differences in subsequent outcomes. The Cox proportional hazards model was used to identify the risk factors for composite endpoints. Kaplan-Meier curves were used to compare survival.

Results: All patients were assigned to either men group (n = 94) or women group (n = 60). Longitudinal follow-ups showed a continuously increasing in 6MWT, Kansas City Cardiomyopathy Questionnaire overall score, EQ-5D visual analogue scale, and EQ-5D Index score in both groups (all P < 0.001); however, women reported a lower level of all parameters at the 1, 6, and 12 months follow-ups (all P < 0.05). In addition, women had a higher risk of all-cause mortality or HF-related hospitalization and a lower cardiac event-free survival rate than men (log-rank test, P = 0.027).

Conclusion: Women reported worse functional capacity, QoL, and prognosis than men in a sample of Chinese patients with HF. Our findings highlight the importance of paying attention to sex differences in HF.
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http://dx.doi.org/10.1097/MD.0000000000029795DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9239662PMC
July 2022

Riluzole regulates pancreatic cancer cell metabolism by suppressing the Wnt-β-catenin pathway.

Sci Rep 2022 Jun 30;12(1):11062. Epub 2022 Jun 30.

Stanley S. Scott Cancer Center, School of Medicine, Louisiana State University Health-New Orleans, New Orleans, LA, 70122, USA.

Most cancer cells rely on aerobic glycolysis to support uncontrolled proliferation and evade apoptosis. However, pancreatic cancer cells switch to glutamine metabolism to survive under hypoxic conditions. Activation of the Wnt/β-catenin pathway induces aerobic glycolysis by activating enzymes required for glucose metabolism and regulating the expression of glutamate transporter and glutamine synthetase. The results demonstrate that riluzole inhibits pancreatic cancer cell growth and has no effect on human pancreatic normal ductal epithelial cells. RNA-seq experiments identified the involvement of Wnt and metabolic pathways by riluzole. Inhibition of Wnt-β-catenin/TCF-LEF pathway by riluzole suppresses the expression of PDK, MCT1, cMyc, AXIN, and CyclinD1. Riluzole inhibits glucose transporter 2 expression, glucose uptake, lactate dehydrogenase A expression, and NAD + level. Furthermore, riluzole inhibits glutamate release and glutathione levels, and elevates reactive oxygen species. Riluzole disrupts mitochondrial homeostasis by inhibiting Bcl-2 and upregulating Bax expression, resulting in a drop of mitochondrial membrane potential. Finally, riluzole inhibits pancreatic cancer growth in KPC (Pdx1-Cre, LSL-Trp53, and LSL-Kras) mice. In conclusion, riluzole can inhibit pancreatic cancer growth by regulating glucose and glutamine metabolisms and can be used to treat pancreatic cancer.
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http://dx.doi.org/10.1038/s41598-022-13472-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9246955PMC
June 2022

Larger-Than-Unity External Optical Field Confinement Enabled by Metamaterial-Assisted Comb Waveguide for Ultrasensitive Long-Wave Infrared Gas Spectroscopy.

Nano Lett 2022 Aug 27;22(15):6112-6120. Epub 2022 Jun 27.

Department of Electrical and Computer Engineering, National University of Singapore, Singapore 117583, Singapore.

Nanophotonic waveguides that implement long optical pathlengths on chips are promising to enable chip-scale gas sensors. Nevertheless, current absorption-based waveguide sensors suffer from weak interactions with analytes, limiting their adoptions in most demanding applications such as exhaled breath analysis and trace-gas monitoring. Here, we propose an all-dielectric metamaterial-assisted comb (ADMAC) waveguide to greatly boost the sensing capability. By leveraging large longitudinal electric field discontinuity at periodic high-index-contrast interfaces in the subwavelength grating metamaterial and its unique features in refractive index engineering, the ADMAC waveguide features strong field delocalization into the air, pushing the external optical field confinement factor up to 113% with low propagation loss. Our sensor operates in the important but underdeveloped long-wave infrared spectral region, where absorption fingerprints of plentiful chemical bonds are located. Acetone absorption spectroscopy is demonstrated using our sensor around 7.33 μm, showing a detection limit of 2.5 ppm with a waveguide length of only 10 mm.
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http://dx.doi.org/10.1021/acs.nanolett.2c01198DOI Listing
August 2022

Glutathione Peroxidase 4 as a Therapeutic Target for Anti-Colorectal Cancer Drug-Tolerant Persister Cells.

Front Oncol 2022 3;12:913669. Epub 2022 Jun 3.

Department of Gastrointestinal Surgery, Peking University Shenzhen Hospital, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center, Shenzhen, China.

Background: Despite the effectiveness of chemotherapy and targeted therapy for colorectal cancer, drug resistance drives therapy failure and tumor relapse. Increasing evidence has suggested that cancer cells can enter a reversible drug-tolerant persister state to survive chemotherapy or targeted agents. However, the traits and treatable vulnerabilities of anti-colorectal cancer drug-tolerant persister cells is not yet known.

Methods: In this study, we established 5-fluorouracil and AZ628-tolerant persister cell models in two colorectal cancer cell lines, namely HCT116 and SW620, and revealed the characteristics of colorectal cancer persister cells by cell viability assay and flow cytometry. We investigated the efficacy and mechanism of ferroptosis inducers RSL3 and FIN56 on persister cells, which are glutathione peroxidase 4 inhibitors. In the xenograft mouse model, we further evaluated the inhibitory effect of RSL3 on tumor regrowth.

Results: Colorectal cancer persister cells, which were enriched in the residual cancer cell population, exhibited reduced drug sensitivity, were largely quiescent and expressed high levels of stem cell-related genes and mesenchymal markers but not epithelial markers. The persister cells were more sensitive and underwent ferroptosis induced by glutathione peroxidase 4 inhibitors. Mechanistically, glutathione peroxidase 4 and ferrous iron, which are pivotal ferroptosis regulators, were upregulated in residual cells or tumors, and were hence potential therapeutic targets of persister cells. In the xenograft model, we confirmed that inhibition of glutathione peroxidase 4 restrained tumor regrowth after discontinuation of anti-cancer drug treatment. Moreover, biopsies obtained from patients with colorectal cancer undergoing neoadjuvant chemoradiotherapy revealed upregulated glutathione peroxidase 4 and ferritin heavy chain 1. High glutathione peroxidase 4 expression correlates with a worse prognosis in colorectal cancer patients.

Conclusions: Our work reveals that the upregulated glutathione peroxidase 4 and ferrous iron in anti-colorectal cancer drug-tolerant persister cells were potential therapeutic targets. Glutathione peroxidase 4 inhibition combined with chemotherapy or targeted therapy may be a promising therapy for colorectal cancer.
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http://dx.doi.org/10.3389/fonc.2022.913669DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9203854PMC
June 2022

Probiotic mixture VSL#3 prevents ulcerative colitis-associated carcinogenesis in mice and cells by regulating the inflammatory and Wnt/β-catenin pathway.

Chin Med J (Engl) 2022 Jun 9. Epub 2022 Jun 9.

Department of Gastroenterology, Key Laboratory of Gut Microbiota Translational Medicine Research, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.

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http://dx.doi.org/10.1097/CM9.0000000000002035DOI Listing
June 2022

Colorectal Cancer Patient-Derived 2D and 3D Models Efficiently Recapitulate Inter- and Intratumoral Heterogeneity.

Adv Sci (Weinh) 2022 08 2;9(22):e2201539. Epub 2022 Jun 2.

School of Pharmaceutical Sciences, Tsinghua University, Beijing, 100084, China.

Pre-existing drug resistance and tumorigenicity of cancer cells are highly correlated with therapeutic failure and tumor growth. However, current cancer models are limited in their application to the study of intratumor functional heterogeneity in personalized oncology. Here, an innovative two-dimensional (2D) and three-dimensional (3D) model for patient-derived cancer cells (PDCCs) and air-liquid interface (ALI) organotypic culture is established from colorectal cancer (CRC). The PDCCs recapitulate the genomic landscape of their parental tumors with high efficiency, high proliferation rate, and long-term stability, while corresponding ALI organotypic cultures retain histological architecture of their original tumors. Interestingly, both 2D and 3D models maintain the transcriptomic profile of the corresponding primary tumors and display the same trend in response to 5-Fluoruracil, regardless of their difference in gene expression profiles. Furthermore, single-cell-derived clones() are efficiently established and pre-existing drug-resistant clones and highly tumorigenic clones within individual CRC tumors are identified. It is found that tumorigenic cancer cells do not necessarily possess the stem cells characteristics in gene expression. This study provides valuable platform and resource for exploring the molecular mechanisms underlying the pre-existing drug resistance and tumorigenicity in cancer cells, as well as for developing therapeutic targets specifically for pre-existing drug-resistant or highly tumorigenic clones.
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http://dx.doi.org/10.1002/advs.202201539DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353492PMC
August 2022

Dynamic experiments of acid mine drainage with Rhodopseudomonas spheroides activated lignite immobilized sulfate-reducing bacteria particles treatment.

Sci Rep 2022 05 24;12(1):8783. Epub 2022 May 24.

College of Civil Engineering, Liaoning Technical University, Fuxin, China.

Aiming at the problem that the treatment of acid mine drainage (AMD) by sulfate-reducing bacteria (SRB) biological method is susceptible to pH, metal ions, sulfate and carbon source. Lignite immobilized SRB particles (SRB-LP) and Rhodopseudomonas spheroides (R. spheroides) activated lignite immobilized SRB particles (R-SRB-LP) were prepared using microbial immobilization technology with SRB, R. spheroides and lignite as the main substrates. The dynamic experimental columns 1# and 2# were constructed with SRB-LP and R-SRB-LP as fillers, respectively, to investigate the dynamic repair effect of SRB-LP and R-SRB-LP on AMD. The mechanism of AMD treated with R-L-SRB particles was analyzed by scanning electron microscopy (SEM), fourier transform infrared (FTIR) spectrometer and low-temperature nitrogen adsorption. The result showed that the combination of R. spheroides and lignite could continuously provide carbon source for SRB, so that the highest removal rates of SO, Cu and Zn in AMD by R-SRB-LP were 93.97%, 98.52% and 94.42%, respectively, and the highest pH value was 7.60. The dynamic repair effect of R-SRB-LP on AMD was significantly better than that of SRB-LP. The characterization results indicated that after R-SRB-LP reaction, the functional groups of -OH and large benzene ring structure in lignite were broken, the lignite structure was destroyed, and the specific surface area was 1.58 times larger than before reaction. It illustrated that R. spheroides provided carbon source for SRB by degrading lignite. The strong SRB activity in R-SRB-LP, SRB can co-treat AMD with lignite, so that the dynamic treatment effect of R-SRB-LP on AMD is significantly better than that of SRB-LP.
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http://dx.doi.org/10.1038/s41598-022-12897-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9130216PMC
May 2022

Global Disease Burden of Respiratory Syncytial Virus in Preterm Children in 2019: A Systematic Review and Individual Participant Data Meta-Analysis Protocol.

J Infect Dis 2022 Aug;226(Supplement_1):S135-S141

Centre for Global Health, Usher Institute, University of Edinburgh, Edinburgh, United Kingdom.

Existing guidelines on respiratory syncytial virus (RSV) prophylaxis differ greatly by gestational age (GA) and other underlying risk factors, highlighting the data gaps in RSV disease burden among preterm infants. We will conduct a systematic review and individual participant data (IPD) meta-analysis of RSV global disease burden among preterm-born children. Three databases, Medline, Embase, and Global Health, will be searched for relevant studies on RSV disease burden for 2019 or before in preterm-born children published between 1 January 1995 and 31 December 2021. IPD will be sought by contacting the investigators identified from published literature and from existing collaboration networks. One-stage and 2-stage random-effects meta-analyses will be used to combine information from IPD and non-IPD studies to produce summary RSV burden estimates of incidence rate, hospital admission rate, and in-hospital case fatality ratio. The framework will be extended to examine subgroup(s) with the most substantial RSV disease burden.
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http://dx.doi.org/10.1093/infdis/jiac078DOI Listing
August 2022

Acceleration of pCASL-Based Cerebral 4D MR Angiography Using Compressed SENSE: A Comparison With SENSE.

Front Neurol 2022 21;13:796271. Epub 2022 Mar 21.

Department of Radiology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, China.

Objectives: The objectives of this study were to accelerate the non-contrast-enhanced four-dimensional magnetic resonance angiography (4D MRA) based on pseudocontinuous arterial spin labeling combined with the Keyhole and View-sharing (4D-PACK) procedure using the Compressed SENSE (C-SENSE) and to improve intracranial vasculopathy evaluations for clinical purposes.

Methods: 4D-PACK acquisition with different C-SENSE and SENSE acceleration factors was performed on 29 healthy volunteers and six patients by means of a 3.0 T MR system. Two radiologists used a 4-grade scale to qualitatively assess the vessel visualization of the middle cerebral artery (MCA) and used a 5-grade scale to qualitatively examine the image quality of 4D-PACK axial source images. Interobserver agreement was assessed by determining the weighted kappa statistic. The contrast-to-noise ratio (CNR) and arterial transmit time (ATT) were calculated in four segments of the MCA. The repeated measures one-way ANOVA for CNR and the Friedman test for source images and vessel visualization were used to analyse the differences in five sequences.

Results: (1) At the M4 segment, C-SENSE5 acquisition (scores, 2.72 ± 0.53) and C-SENSE6.5 (scores, 2.55 ± 0.57) provided similar vessel visualization compared with SENSE4.5 (scores, 2.72 ± 0.46); however, C-SENSE8 (scores, 1.79 ± 0.49) and C-SENSE10 (scores, 1.52 ± 0.51) had lower scores ( < 0.050). (2) The source image quality of C-SENSE5 (scores, 4.55 ± 0.51), C-SENSE6.5 (scores, 4.03 ± 0.33), and C-SENSE8 (scores, 3.48 ± 0.51) acquisition was higher than that of SENSE4.5 (scores, 3.07 ± 0.26) ( < 0.001). (3) CNRs of different MCA segments for C-SENSE5 and C-SENSE6.5 acquisitions were not significantly different compared with that of SENSE4.5 acquisition. However, the CNRs were significantly lower for C-SENSE8 (M1: 45.85 ± 13.91, M2: 27.08 ± 9.92, M4: 7.93 ± 4.49) and C-SENSE10 (M1: 37.94 ± 9.92, M2: 23.51 ± 9.0, M4: 6.78 ± 4.12) than for SENSE4.5 (M1: 55.49 ± 13.39, M2: 36.94 ± 11.02, M4: 10.18 ± 5.15) in each corresponding segment ( < 0.050). ATTs in all MCA segments within different accelerating C-SENSE factors were obviously correlated with SENSE4.5.

Conclusion: C-SENSE6.5 acquisition could be used to evaluate both the intracranial macrovascular and distal arteries, which could reduce the acquisition time by 18% (5 min 5 s) compared with SENSE4.5. Moreover, C-SENSE8 acquisition (37% acceleration, 3 min 54 s) could be used for routine screening and clinical diagnosis of intracranial macrovascular disease with balanced image quality.
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http://dx.doi.org/10.3389/fneur.2022.796271DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8977489PMC
March 2022

Herbal inhibitors of SARS-CoV-2 M effectively ameliorate acute lung injury in mice.

IUBMB Life 2022 06 22;74(6):532-542. Epub 2022 Apr 22.

State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, Department of Biochemistry, School of Basic Medicine Peking Union Medical College, Beijing, China.

Coronavirus disease 2019, a newly emerging serious infectious disease, has spread worldwide. To date, effective drugs against the disease are limited. Traditional Chinese medicine was commonly used in treating COVID-19 patients in China. Here we tried to identify herbal effective lipid compounds from the lipid library of 92 heat-clearing and detoxication Chinese herbs. Through virtual screening, enzymatic activity and inhibition assays, and surface plasmon resonance tests, we identified lipid compounds targeting the main protease (M ) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and verified their functions. Here, we found that natural lipid compounds LPC (14:0/0:0) and LPC (16:0/0:0) could target SARS-CoV-2 M , recover cell death induced by SARS-CoV-2, and ameliorate acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) induced by bacterial lipopolysaccharides and virus poly (I:C) mimics in vivo and in vitro. Our results suggest that LPC (14:0/0:0) and LPC (16:0/0:0) might be potential pan remedy against ARDS.
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http://dx.doi.org/10.1002/iub.2616DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9087564PMC
June 2022

Effect of pirfenidone protecting against cigarette smoke extract induced apoptosis.

Tob Induc Dis 2022 1;20:24. Epub 2022 Mar 1.

Department of Respiratory and Critical Care Medicine, The Second Xiangya Hospital, Central South University, Changsha, China.

Introduction: Apoptosis of lung structural cells is a significant upstream event involved in COPD pathogenesis. This study was designed to explore whether pirfenidone (PFD) was able to attenuate apoptosis induced by cigarette smoke extract (CSE).

Methods: A method of intraperitoneal CSE injection to BALB/C mice was used to establish emphysema mouse model. Terminal deoxynucleotidyl transferase dUTPnick end labeling (TUNEL) assay was applied to evaluate apoptotic cell ratio in mouse lung tissue. The cell viability of HBECs exposed to different concentrations of PFD was measured by Cell Counting Kit-8 (CCK-8) assay. The apoptosis index (AI) of HBECs was tested by flow cytometry. Levels of apoptosis-related protein were determined by Western blotting.

Results: PFD treatment significantly decreased the AI value in emphysema mouse lung tissue by TUNEL. In HBECs, flow cytometry showed that PFD could significantly reduce AI led by CSE. Both and , protein levels of Bax and Cleaved-caspase 3 in CSE group significantly increased in contrast with the control group; while Bcl-2 protein level in CSE group was significantly decreased; moreover, PFD significantly reversed protein level changes of Bcl-2, Bax, and Cleaved-caspase 3 led by CSE.

Conclusions: This study reveals that PFD may potentially protect against CSE induced apoptosis.
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http://dx.doi.org/10.18332/tid/146169DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8886422PMC
March 2022

Emerging Therapeutic Potential of Mesenchymal Stem Cell-Derived Extracellular Vesicles in Chronic Respiratory Diseases: An Overview of Recent Progress.

Front Bioeng Biotechnol 2022 25;10:845042. Epub 2022 Feb 25.

Department of Pulmonary and Critical Care Medicine, The Second Xiangya Hospital, Central South University, Changsha, China.

Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) are able to carry genetic and protein goods to mediate the interaction between MSCs and target cells. Recently, more and more researches have focused on the therapeutic role of MSC-EVs in chronic respiratory diseases. In this review, we summarize the cumulative strategies and mechanisms of MSC-EVs in treating chronic respiratory diseases. This review suggests that MSC-EVs may serve as a novel cell-free-based therapy for chronic respiratory diseases, including COPD, asthma, pulmonary fibrosis, and pulmonary arterial hypertension. In current studies of chronic respiratory diseases, umbilical cord and bone marrow are main sources of MSC-EVs, while adipose tissue, lung, and induced pluripotent stem cells are also applied. Isolation methods of MSC-EVs in treating chronic respiratory diseases involve ultracentrifugation, exosome extraction kits and anion-exchange chromatography. Intratracheal delivery and intravenous administration are the most widely used routes of MSC-EVs. MSC-EVs are able to transfer microRNAs and protein to target cells and further magnify the therapeutic effects.
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http://dx.doi.org/10.3389/fbioe.2022.845042DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8913891PMC
February 2022

Synthesis of K[BHNHBHNHBH] for a K-ion solid-state electrolyte.

Chem Commun (Camb) 2022 Mar 29;58(26):4200-4203. Epub 2022 Mar 29.

School of Chemistry and Chemical Engineering, Henan Key Laboratory of Boron Chemistry and Advanced Energy Materials, Henan Normal University, Xinxiang, Henan 453007, China.

All-solid-state K batteries are ideal energy storage devices for grid-scale applications of renewable energies. A novel electrolyte K[BHNHBHNHBH] with weakly coordinating anions was synthesized. It has a high K conductivity of 1.01 × 10 S cm at 75 °C, which is probably due to the increased electrostatic potential and size of the anions.
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http://dx.doi.org/10.1039/d2cc00408aDOI Listing
March 2022

Bimetallic sulfide NiCoS yolk-shell nanospheres as high-performance cathode materials for rechargeable magnesium batteries.

Nanoscale 2022 Mar 24;14(12):4753-4761. Epub 2022 Mar 24.

State Key Laboratory of Advanced Electromagnetic Engineering and Technology, School of Electrical and Electronic Engineering, Huazhong University of Science and Technology, Wuhan 430074, P. R. China.

Constructing bimetallic sulfides with ideal structures could effectively alleviate the poor cycling stability of rechargeable Mg batteries due to a bimetallic synergistic effect. An exquisite yolk-shell structured bimetallic sulfide NiCoS synthesized a two-step solvothermal hydrothermal method is investigated as a cathode material for rechargeable Mg batteries. With the bimetallic strategy and well-designed architecture, the as-synthesized yolk-shell NiCoS exhibits outstanding Mg-storage performance, demonstrating a superior reversible capacity (270 mA h g at 50 mA h g), a high rate capability, and a specific capacity retention of 91% over 400 cycles (2.2% capacity decay per cycle). The in-depth mechanism investigation reveals the two-step conversion reaction process and the bimetallic synergistic effect in the Mg-storage process. Based on DFT calculations and kinetic investigations, the bimetallic synergistic effect effectively alleviates the Jahn-Teller effect and distortion in the crystal lattices and increases active reaction sites, thus largely enhancing the electrochemical Mg-storage performance. The superior electrochemical performance of NiCoS not only demonstrates the viability of the bimetallic strategy but also sheds light on the use of nanostructure design for high-performance cathode research.
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http://dx.doi.org/10.1039/d2nr00128dDOI Listing
March 2022

Endothelial Microparticles Derived from Primary Pulmonary Microvascular Endothelial Cells Mediate Lung Inflammation in Chronic Obstructive Pulmonary Disease by Transferring microRNA-126.

J Inflamm Res 2022 28;15:1399-1411. Epub 2022 Feb 28.

Department of Respiratory and Critical Care Medicine, The Second Xiangya Hospital of Central South University, Changsha, People's Republic of China.

Background: Extracellular vesicles (EVs) are considered to new types of intercellular communication media, and microRNA is one of the most common transferring components of EVs. This study aimed to explore the potential role of endothelial microparticles (EMPs) derived from primary pulmonary microvascular endothelial cells in regulating lung inflammation of chronic obstructive pulmonary disease (COPD) through transferring microRNA-126 (miR-126).

Methods: EMPs generated from primary pulmonary microvascular endothelial cells were isolated by gradient centrifugation and characterized by transmission electron microscopy, flow cytometry and Western blotting. EMPs were treated to in vitro and in vivo COPD models induced by cigarette smoke extract (CSE). miR-126 mimics or inhibitors were transfected into EMPs by calcium chloride. Pathological changes of lung tissue, mRNA and protein levels of inflammation-related factors were measured to explore the effect of EMPs transferring miR-126 on CSE-induced inflammation.

Results: Both in vitro and in vivo studies demonstrated that mRNA and protein levels of inflammation-related factors were significantly increased in COPD group, while EMPs could dramatically reverse these increases. In vitro, overexpression of miR-126 in EMPs decreased HMGB1 expression and magnified the decreasing effect of EMPs on inflammation-related factors.

Conclusion: The present study reveals that EMPs are capable of alleviating lung inflammation and transferring miR-126 can magnify the anti-inflammatory effect of EMPs, which may provide a novel therapeutic alternative for COPD.
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http://dx.doi.org/10.2147/JIR.S349818DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8896043PMC
February 2022

Constitutive β-Catenin Overexpression Represses Lncrna MIR100HG Transcription via HDAC6-Mediated Histone Modification in Colorectal Cancer.

Mol Cancer Res 2022 06;20(6):949-959

State Key Laboratory of Molecular Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Wnt/β-catenin signaling plays a critical role in colonic carcinogenesis. However, non-coding RNAs (ncRNA) transcriptionally regulated by β-catenin are largely unknown. Herein, we found that lncRNA MIR100HG (lnc-MIR100HG) negatively correlated with target genes of β-catenin from The Cancer Genome Atlas colorectal carcinoma database, which was verified in 48 paired colorectal carcinoma specimens. In addition, constitutive overexpression of β-catenin decreased primary and mature lnc-MIR100HG levels, whereas blockage of β-catenin activity with siRNA or inhibitors significantly increased their expression. DNA pull-down and chromatin immunoprecipitation revealed the binding of β-catenin/TCF4 to the MIR100HG promoter. Moreover, β-catenin-forced expression reduced the enrichment of H3K27Ac, an active transcription marker, on the promoter, whereas β-catenin inhibition reversed this effect. Furthermore, HDAC6 was recruited to the MIR100HG promoter and downregulated H3K27Ac enrichment in a β-catenin-dependent manner. Besides, HDAC6 was upregulated and negatively correlated with lnc-MIR100HG in colorectal carcinoma specimens. Functional studies showed that lnc-MIR100HG overexpression induced cell-cycle G0-G1 arrest and repressed cell proliferation via p57 upregulation in vitro and in vivo. Taken together, we found that ectopic β-catenin transcriptionally repressed lnc-MIR100HG expression through HDAC6-mediated histone modification in colorectal carcinoma. Lnc-MIR100HG regulates the cell cycle through p57.

Implications: It provides a novel downstream mechanism highlighting β-catenin action during colon carcinogenesis and may shed light for further therapeutic approaches.
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http://dx.doi.org/10.1158/1541-7786.MCR-21-0923DOI Listing
June 2022

Surrogate-Assisted Fine Particulate Matter Exposure Assessment in an Underground Subway Station.

Int J Environ Res Public Health 2022 02 17;19(4). Epub 2022 Feb 17.

State Key Laboratory of Advanced Electromagnetic Engineering and Technology, School of Electrical and Electronic Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.

With the increase in subway travelers, the air quality of underground enclosed spaces at subway stations has attracted much more attention. The study of pollutants exposure assessment, especially fine particulate matter, is important in both pollutant control and metro station design. In this paper, combining pedestrian flow analysis (PFA) and computational fluid dynamics (CFD) simulations, a novel surrogate-assisted particulate matter exposure assessment method is proposed, in which PFA is used to analyze the spatial-temporal movement characteristics of pedestrians to simultaneously consider the location and value of the pedestrian particulate generation source and their exposure streamline to particulate matter; the CFD model is used to analyze the airflow field and particulate matter concentration field in detail. To comprehensively consider the differences in the spatial concentration distribution of particulate matter caused by the time-varying characteristics of the airflow organization state in subway stations, surrogate models reflecting the nonlinear relationship between simulated and measured data are trained to perform accurate pedestrian exposure calculations. The actual measurement data proves the validity of the simulation and calculation methods, and the difference between the calculated and experimental values of the exposure is only about 5%.
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http://dx.doi.org/10.3390/ijerph19042295DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8872296PMC
February 2022

In Situ Observation of Microstructural and Inclusions Evolution in High-Strength Steel Deposited Metals with Various Rare Earth Pr Contents.

Materials (Basel) 2022 Feb 8;15(3). Epub 2022 Feb 8.

Department of Materials Science and Engineering, University of Wisconsin, Madison, WI 53706, USA.

The evolution of austenite, acicular ferrite, upper bainite and martensite, and the nucleation of inclusions in the microstructure of high-strength steel deposited metals, was systematically investigated using three kinds of A5.28 E120C-K4 metal-cored wires with various rare earth Pr contents. Grain structure evolution in the process of high temperature, dispersoid characteristics of inclusions and the crystallographic characteristics of the microstructure were assessed. Compared with no addition of PrO, adding 1%PrO resulted in refined, spheroidized and dispersed inclusions in the deposited metal, leading to an increase in the pinning forces on the grain boundary movement, promoting the formation of an ultra-fine grain structure with an average diameter of 41 μm. The inclusions in the deposited metals were Mn-Si-Pr-Al-Ti-O after Pr addition; the average size of the inclusions in the Pr-containing deposited metals was the smallest, while the number and density of inclusions was the highest. The size of effective inclusions (nucleus of acicular ferrite formation) was mainly in the range of 0.6-1.5 μm. In addition, the content of upper bainite decreased, while the percentage of acicular ferrite increased by 24% due to the increase in the number of effective inclusions in the Pr-containing deposited metals in this study. This study shows that the addition of 1% PrO is efficient in achieving fine interlaced multiphase with an ultrafine-grained structure, resulting in an enhancement of the impact toughness of the deposited metal.
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http://dx.doi.org/10.3390/ma15031257DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8838063PMC
February 2022

Experimental Study on Carbonation Durability of Kaolin Strengthened with Slag Portland Cement.

Materials (Basel) 2022 Feb 7;15(3). Epub 2022 Feb 7.

College of Resources, Shandong University of Science and Technology, Tai'an 271019, China.

Slag Portland cement is an environmentally friendly and energy-saving product, which is widely used in cement-reinforced soil. This study used slag Portland cement-reinforced soil as the research object and P.O 42.5 + kaolin (POK) as the reference group. The carbonation depth and strength of P.S.A 42.5 + kaolin (PSK) at different curing times were analyzed using carbonation depth, uniaxial ground pressure strength, scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS) and X-ray diffraction (XRD). The test results show the following: (1) The active substances in PSK samples can react with calcium hydroxide produced during cement hydration and can reduce the content of OH. The PSK samples react with OH and CO in the carbonation environment. Both processes considerably reduce the content of OH. (2) Due to the decrease in OH content, the carbonation durability of slag Portland cement-reinforced soil is significantly less than that of ordinary Portland cement. (3) The carbonation of slag Portland cement-reinforced soil improves its strength. (4) The results of SEM + EDS and XRD confirm the carbonation depth and strength of the POK and PSK samples. The results show that PSK has important applications in subgrade or building grouting materials and in cement-soil mixing piles (walls).
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http://dx.doi.org/10.3390/ma15031240DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8839291PMC
February 2022

Chronic alcohol exposure induces hepatocyte damage by inducing oxidative stress, SATB2 and stem cell-like characteristics, and activating lipogenesis.

J Cell Mol Med 2022 04 13;26(7):2119-2131. Epub 2022 Feb 13.

Kansas City VA Medical Center, Kansas City, Missouri, USA.

Alcohol is a risk factor for hepatocellular carcinoma (HCC). However, the molecular mechanism by which chronic alcohol consumption contributes to HCC is not well understood. The purpose of the study was to demonstrate the effects of chronic ethanol exposure on the damage of human normal hepatocytes. Our data showed that chronic exposure of hepatocytes with ethanol induced changes similar to transformed hepatocytes that is, exhibited colonies and anchorage-independent growth. These damaged hepatocytes contained high levels of reactive oxygen species (ROS) and showed induction of the SATB2 gene. Furthermore, damaged hepatocytes gained the phenotypes of CSCs which expressed stem cell markers (CD133, CD44, CD90, EpCAM, AFP and LGR5), and pluripotency maintaining factors (Sox-2, POU5F1/Oct4 and KLF-4). Ethanol exposure also induced Nanog, a pluripotency maintaining transcription factor that functions in concert with Oct4 and SOX-2. Furthermore, ethanol induced expression of EMT-related transcription factors (Snail, Slug and Zeb1), N-Cadherin, and inhibited E-cadherin expression in damaged hepatocytes. Ethanol enhanced recruitment of SATB2 to promoters of Bcl-2, Nanog, c-Myc, Klf4 and Oct4. Ethanol also induced activation of the Wnt/TCF-LEF1 pathway and its targets (Bcl-2, Cyclin D1, AXIN2 and Myc). Finally, ethanol induced hepatocellular steatosis, SREBP1 transcription, and modulated the expression of SREBP1c, ACAC, ACLY, FASN, IL-1β, IL-6, TNF-α, GPC3, FLNB and p53. These data suggest that chronic alcohol consumption may contribute towards the development of HCC by damaging normal hepatocytes with the generation of inflammatory environment, induction of SATB2, stem cell-like characteristics, and cellular steatosis.
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http://dx.doi.org/10.1111/jcmm.17235DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8980954PMC
April 2022

Clinical and economic burden of comorbid coronary artery disease in patients with acute exacerbation of chronic obstructive pulmonary disease: sex differences in a nationwide cohort study.

Respir Res 2022 Feb 12;23(1):28. Epub 2022 Feb 12.

Department of Pulmonary and Critical Care Medicine, China-Japan Friendship Hospital, Beijing, China.

Background: Coronary artery disease (CAD) is a common comorbidity of chronic obstructive pulmonary disease (COPD). However, data related to the impact of CAD on outcomes of acute exacerbation of COPD (AECOPD) are limited and whether the relationship depends on sex remains unknown. Our aim was to determine the impact of comorbid CAD on clinical outcomes among men and women with AECOPD.

Methods: We used data from the acute exacerbation of chronic obstructive pulmonary disease inpatient registry (ACURE) study, which is a nationwide observational real-world study conducted between September 2017 and February 2020 at 163 centers in patients admitted with AECOPD as their primary diagnosis. Patients were stratified according to the presence or absence of CAD in men and women. The primary outcomes were the length of hospital stay and economic burden during hospitalization.

Results: Among 3906 patients included in our study, the prevalence of CAD was 17.0%, and it was higher in women than in men (19.5% vs. 16.3%; P = 0.034). Age and other cardiovascular diseases were common factors associated with comorbid CAD in men and women, while body-mass index, cerebrovascular disease, and diabetes were determinants in men and pre-admission use of long-acting beta-adrenoceptor agonist and home oxygen therapy were protective factors in women. Only in men, patients with CAD had a longer length of hospital stay (median 10.0 vs. 9.0 days, P < 0.001), higher total cost during hospitalization (median $1502.2 vs. $1373.4, P < 0.001), and more severe COPD symptoms at day 30 compared to those without CAD. No significant difference was found in women. Comorbid CAD showed no relationship with 30-day readmission or death regardless of sex. In our real-world study, mortality/readmission risk within 30 days increased in patients with previous frequent hospitalizations and poorer pulmonary function.

Conclusions: In hospitalized AECOPD patients, comorbid CAD was significantly associated with poorer short-term outcomes in men. Clinicians should have heightened attention for men with comorbid CAD to achieve an optimal management of AECOPD patients.
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http://dx.doi.org/10.1186/s12931-022-01945-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8840293PMC
February 2022

Methylxanthine Treatment in Patients Hospitalized for Acute Exacerbation of Chronic Obstructive Pulmonary Disease in China: A Real-World Study Using Propensity Score Matching Analysis.

Front Pharmacol 2022 25;13:802123. Epub 2022 Jan 25.

Department of Pulmonary and Critical Care Medicine, China-Japan Friendship Hospital, Beijing, China.

Although medical guidelines discourage the use of methylxanthines in patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD), they are still widely used in clinical practice. This study investigated the real-world use of methylxanthines in the management of AECOPD. Patient data from the Acute exacerbation of Chronic obstructive pulmonary disease Using REgistry data (ACURE, NCT02657525) study database were screened. Enrolled patients were divided into treatment and control groups. Propensity score (PS) matching and Cox regression analyses were used to minimize confounding factors and determine the association between methylxanthine treatment and the length of stay (LOS). Among the 2088 eligible patients, 1,563 (74.9%) were in the methylxanthine treatment group. Patients treated with methylxanthines had more severe respiratory symptoms and worse lung function than those in the control group. Doxophylline was the most commonly used methylxanthine in both secondary and tertiary hospitals. After PS matching, 966 patients were equally divided into two groups. The LOS of patients in the two groups was similar [median: 8 days, interquartile range (IQR): 7-11 days, = 0.730]. Patients in the treatment group (median: 8, IQR: 4-12) had a more significant decrease in the COPD Assessment Test score from admission to discharge than those in the control group (median: 6, IQR: 2-10, < 0.001). Among all matched patients, the LOS was not significantly associated with methylxanthine treatment [adjusted hazard ratio (HR): 1.02, 95% confidence intervals (CIs): 0.89-1.16]. However, in the subgroup analysis, methylxanthines were significantly associated with a short LOS in patients with blood eosinophil count >4% (adjusted HR: 1.56, 95% CIs: 1.12-2.17). This study revealed that methylxanthines, especially doxophylline, are widely used in China. Methylxanthines were effective in improving symptoms in AECOPD patients. Higher blood eosinophil count may be associated with a better efficacy of methylxanthine treatment.
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http://dx.doi.org/10.3389/fphar.2022.802123DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8821534PMC
January 2022

2'-Fucosyllactose promotes Lactobacillus rhamnosus KLDS 8001 to repair LPS-induced damage in Caco-2 cells.

J Food Biochem 2022 05 3;46(5):e14059. Epub 2022 Feb 3.

Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin, China.

The aim of this study was to investigate the effect of 2'-fucosyllactose (2'-FL) on the repair of monolayer barrier damage in Caco-2 cells by Lactobacillus rhamnosus KLDS 8001 (KLDS 8001). The results showed that the addition of 2'-FL not only promoted the adhesion ability of KLDS 8001 to Caco-2 cells but also improved the anti-adhesive effect of pathogenic bacteria. Compared with 2'-FL or KLDS 8001 alone, 2'-FL+KLDS 8001 significantly reduced lipopolysaccharide (LPS)-induced malondialdehyde (MDA), lactate dehydrogenase (LDH) release, and cytokine (IL-1β, IL-6, and TNF-α) production. In addition, 2'-FL effectively promoted the transmembrane electrical resistance (TEER), cell viability, and cellular permeability of KLDS 8001 repaired damaged cells with dose-dependent properties. The mRNA and protein expression of Zonula Occludens-1 (ZO-1), Occludin, and Claudin-1 were also upregulated in the KLDS 8001 and 2'-FL co-treated treatment group. It was speculated that 2'-FL could effectively regulate the interaction between KLDS 8001 and intestinal epithelial cells to play a role in maintaining intestinal barrier function and avoiding pathogenic bacteria invasion. PRACTICAL APPLICATIONS: As the most widely used human milk oligosaccharides (HMOs), 2'-FL is vital for maintaining infant intestinal health. Our study found that the addition of 2'-FL promoted KLDS 8001 adhesion, anti-adhesion of pathogenic bacteria, anti-inflammatory capacity, repair of barrier damage, and tight junction protein expression, providing a new strategy to protect infant intestinal health and prevent various intestinal diseases.
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http://dx.doi.org/10.1111/jfbc.14059DOI Listing
May 2022

Extracorporeal Cardiac Shock Wave-Induced Exosome Derived From Endothelial Colony-Forming Cells Carrying miR-140-3p Alleviate Cardiomyocyte Hypoxia/Reoxygenation Injury the PTEN/PI3K/AKT Pathway.

Front Cell Dev Biol 2021 10;9:779936. Epub 2022 Jan 10.

Department of Cardiology, The First Affiliated Hospital of Kunming Medical University, Kunming, China.

Stem cell-derived exosomes have great potential in the treatment of myocardial ischemia-reperfusion injury (IRI). Extracorporeal cardiac shock waves (ECSW) as effective therapy, in part, could activate the function of exosomes. In this study, we explored the effect of ECSW-induced exosome derived from endothelial colony-forming cells on cardiomyocyte hypoxia/reoxygenation (H/R) injury and its underlying mechanisms. The exosomes were extracted and purified from the supernatant of endothelial colony-forming cells (ECFCs-exo). ECFCs-exo treated with shock wave (SW-exo) or without shock wave (CON-exo) were performed with high-throughput sequencing of the miRNA. H9c2 cells were incubated with SW-exo or CON-exo after H/R injury. The cell viability, cell apoptosis, oxidative stress level, and inflammatory factor were assessed. qRT-PCR was used to detect the expression levels of miRNA and mRNA in cells and exosomes. The PTEN/PI3K/AKT pathway-related proteins were detected by Western blotting, respectively. Exosomes secreted by ECFCs could be taken up by H9c2 cells. Administration of SW-exo to H9c2 cells after H/R injury could significantly improve cell viability, inhibit cell apoptosis, and downregulate oxidative stress level (< 0.01), with an increase in Bcl-2 protein and a decrease in Bax, cleaved caspase-3, and NF-κB protein (< 0.05). Notably, miR-140-3p was found to be highly enriched both in ECFCs and ECFCs-exo treated with ECSW (< 0.05) and served as a critical mediator. SW-exo increased miR-140-3p expression but decreased PTEN expression in H9c2 cells with enhanced phosphorylation of the PI3K/AKT signaling pathway. These cardioprotective effects of SW-exo on H/R injury were blunted by the miR-140-3p inhibitor. Dual-luciferase assay verified that miR-140-3p could directly target the 3'UTR of PTEN mRNA and exert a negative regulatory effect. This study has shown the potential of ECSW as an effective stimulation for the exosomes derived from ECFCs . SW-exo exerted a stronger therapeutic effect on H/R injury in H9c2 cells possibly delivering exosomal miR-140-3p, which might be a novel promising strategy for the myocardial IRI.
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http://dx.doi.org/10.3389/fcell.2021.779936DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8784835PMC
January 2022
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