Publications by authors named "Yiman Liu"

19 Publications

  • Page 1 of 1

Controllable Design of Bifunctional VO Coatings with Superhydrophobic and Thermochromic Performances.

ACS Appl Mater Interfaces 2021 Mar 10;13(11):13751-13759. Epub 2021 Mar 10.

Functional Nanomaterials Laboratory, Center for Micro/Nanomaterials and Technology, and Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

The structure and functions of natural organisms provide great inspirational sources for designing and manufacturing bionic coatings, which hold a distinguished scientific promise to tackle challenges facing humans. In this work, we report a facile and controllable approach to prepare various hexagonal periodic array VO thin films by simply manipulating the speed of the dip-coating operation. The hexagonal cellular-structured VO surface delivered the best thermochromic performance with a of 79.34% and a Δ of 9.87%. Impressively, superhydrophobic and thermochromic properties could be integrated into hexagonal semi-dome thin films (with a of 70.9%, a Δ of 9.3%, and a water contact angle of 150°) without any post-treatment by low-surface-energy chemicals, which hold considerable potential for application in multifunctional smart windows. Moreover, based on the Cassie-Baxter mode and finite-difference time-domain calculations, the dependence of the thermochromic and wettability performances on the VO structure has been investigated in this study.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.0c21491DOI Listing
March 2021

Anticancer activities of TCM and their active components against tumor metastasis.

Biomed Pharmacother 2021 Jan 11;133:111044. Epub 2020 Dec 11.

State Key Laboratory of Component-based Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China. Electronic address:

Traditional Chinese Medicine (TCM) has the characteristics of multiple targets, slight side effects and good therapeutic effects. Good anti-tumor effects are shown by Traditional Chinese Medicine prescription, Chinese patent medicine, single Traditional Chinese Medicine and Traditional Chinese medicine monomer compound. Clinically, TCM prolonged the survival time of patients and improved the life quality of patients, due to less side effects. Cancer metastasis is a complex process involving numerous steps, multiple genes and their products. During the process of tumor metastasis, firstly, cancer cell increases its proliferative capacity by reducing autophagy and apoptosis, and then the cancer cell capacity is stimulated by increasing the ability of tumors to absorb nutrients from the outside through angiogenesis. Both of the two steps can increase tumor migration and invasion. Finally, the purpose of tumor metastasis is achieved. By inhibiting autophagy and apoptosis of tumor cells, angiogenesis and EMT outside the tumor can inhibit the invasion and migration of cancer, and consequently achieve the purpose of inhibiting tumor metastasis. This review explores the research achievements of Traditional Chinese Medicine on breast cancer, lung cancer, hepatic carcinoma, colorectal cancer, gastric cancer and other cancer metastasis in the past five years, summarizes the development direction of TCM on cancer metastasis research in the past five years and makes a prospect for the future.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biopha.2020.111044DOI Listing
January 2021

A Current Sensing Biosensor for BOD Rapid Measurement.

Archaea 2020 26;2020:8894925. Epub 2020 Oct 26.

School of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao, 266033, China.

In order to improve the practicality of the rapid biochemical oxygen demand (BOD) method, a highly sensitive rapid detection method for BOD that is based on establishing the correlation between current and dissolved oxygen (DO) was developed. In this experiment, was used as the test microorganism, and the embedding method was used to achieve quantitative fixation of microorganisms, which could increase the content of microorganisms and prolong the service life of the biological element. The conductivity (COND) probe is used as a sensing element, so that the testing value can be read every second. In the program, the moving average method is used to process the collected data so that the value can be read every minute. National standard samples were detected to test the accuracy and stability of the method. The results showed that relative error and analytical standard deviations were less than 5%. Different polluted water was tested to evaluate its application range. The results showed that relative error was less than 5%. The results of the method are consistent with the results of the wastewater sample obtained by the BOD standard method. The proposed rapid BOD current sensing biosensor method should be promising in practical application of wastewater monitoring.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2020/8894925DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7641274PMC
October 2020

Cellular senescence and cancer: Focusing on traditional Chinese medicine and natural products.

Cell Prolif 2020 Oct 3;53(10):e12894. Epub 2020 Sep 3.

Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.

Cancer is the principal cause of death and a dominant public health problem which seriously threatening human life. Among various ways to treat cancer, traditional Chinese medicine (TCM) and natural products have outstanding anti-cancer effects with their unique advantages of high efficiency and minimal side effects. Cell senescence is a physiological process of cell growth stagnation triggered by stress, which is an important line of defence against tumour development. In recent years, active ingredients of TCM and natural products, as an interesting research hotspot, can induce cell senescence to suppress the occurrence and development of tumours, by inhibiting telomerase activity, triggering DNA damage, inducing SASP, and activating or inactivating oncogenes. In this paper, the recent research progress on the main compounds derived from TCM and natural products that play anti-cancer roles by inducing cell senescence is systematically reviewed, aiming to provide a reference for the clinical treatment of pro-senescent cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/cpr.12894DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7574878PMC
October 2020

Bioavailability and Bioaccumulation of 6:2 Fluorotelomer Sulfonate, 6:2 Chlorinated Polyfluoroalkyl Ether Sulfonates, and Perfluorophosphinates in a Soil-Plant System.

J Agric Food Chem 2020 Apr 3;68(15):4325-4334. Epub 2020 Apr 3.

College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi Province 712100, P. R. China.

As emerging alternatives of legacy perfluoroalkyl substances, 6:2 fluorotelomer sulfonate (6:2 FTS), 6:2 chlorinated polyfluoroalkyl ether sulfonates (6:2 Cl-PFESA), and perfluorophosphinates (C6/C6 and C8/C8 PFPiAs) are supposed to be partitioned to soil and highly persistent in the environment. The uptake of novel per- and polyfluoroalkyl substances (PFASs) by plants represents a potential pathway for their transfer in the food chain. In this study, the bioavailability of these four novel PFASs in soil and the bioaccumulation characteristics in greenhouse-grown wheat ( L.), maize ( L.), soybean ( L. Merrill), and pumpkin ( L.) were investigated. The results indicated that these novel PFASs with higher hydrophobicity were more easily sequestrated in soil, and the fractions extracted by methanol could well describe their bioavailability, which could be stimulated by low-molecular-weight organic acids at rhizospheric concentrations. A negative relationship was found between root soil concentration factors (RSCFs) and hydrophobicity (log ) of the target PFASs. This correlation was also found in the translocation factors (TF) from roots to shoots. Furthermore, the uptake and transfer of the target PFASs were regulated by the protein contents in plant roots and shoots.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jafc.0c00542DOI Listing
April 2020

The transport of graphitic carbon nitride in saturated porous media: Effect of hydrodynamic and solution chemistry.

Chemosphere 2020 Jun 22;248:125973. Epub 2020 Jan 22.

College of Natural Resources and Environment, Northwest A&F University, Yangling, 712100, China; Key Laboratory of Plant Nutrition and the Agri-environment in Northwest China, Ministry of Agriculture and Rural Affairs, Yangling, 712100, China.

Understanding transport behavior of graphitic carbon nitride (g-CN) in porous media plays an important role in preventing its possible causing the underground environmental problems. The transport behavior of g-CN in porous media were investigated by packed column experiments at different flow rates, ionic strengths (ISs), pHs and multivalent cations. The experimental results showed that the transport ability of g-CN decreased with the IS increasing, and most of the g-CN was retained in the sand column for the IS greater than 0.0001 M. The flow rate had little effect on the transport behavior of g-CN, and the recovery of g-CN increased slightly with increasing flow rate. In addition, the migration ability of g-CN under acidic conditions was drastically reduced compared with neutral alkaline conditions. Moreover, it was found that 1.51%, 30.33%, 34.91%, and 60.54% of g-CN was retained in the column when g-CN was leached through the quartz sand column at Al, Ca, Mg, and K, which was consistent with the Schulze-Hardy rule. Finally, FTIR spectrum showed that the infrared absorption peak of the g-CN mixed quartz sand were shifted to certain degrees under different conditions, which confirmed that hydrogen bond was formed in the transport of carbon nitride with the quartz sand surface. This study provides a new perspective on the role of hydrogen bond in the transport and fate of nanomaterials.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.chemosphere.2020.125973DOI Listing
June 2020

Upregulation of BCAM and its sense lncRNA BAN are associated with gastric cancer metastasis and poor prognosis.

Mol Oncol 2020 04 13;14(4):829-845. Epub 2020 Feb 13.

Department of Cell Biology and Department of Gastroenterology of Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Patients with metastatic gastric cancer (GC) have a poor prognosis; however, the molecular mechanism of GC metastasis remains unclear. Here, we employed bioinformatics to systematically screen the metastasis-associated genes and found that the levels of basal cell adhesion molecule (BCAM) were significantly increased in GC tissues from patients with metastasis, as compared to those without metastasis. The upregulation of BCAM was also significantly associated with a shorter survival time. Depletion of BCAM inhibited GC cell migration and invasion. Knockout (KO) of BCAM by the CRISPR/Cas9 system reduced the invasion and metastasis of GC cells. To explore the mechanism of BCAM upregulation, we identified a previously uncharacterized BCAM sense lncRNA that spanned from exon 6 to intron 6 of BCAM, and named it as BCAM-associated long noncoding RNA (BAN). Knockdown of BAN inhibited BCAM expression at both mRNA and protein levels. Knockdown of BAN suppressed GC cell migration and invasion, which was effectively rescued by ectopic expression of BCAM. Further clinical data showed that BAN upregulation was associated with GC metastasis and poor prognosis. Importantly, BAN expression was also significantly associated with that of BCAM in GC tissues. Taken together, these results indicate that increased expression of BCAM and its sense lncRNA BAN promote GC cell invasion and metastasis, and are associated with poor prognosis of GC patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/1878-0261.12638DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7138403PMC
April 2020

Insights into Uptake, Translocation, and Transformation Mechanisms of Perfluorophosphinates and Perfluorophosphonates in Wheat ( L.).

Environ Sci Technol 2020 01 17;54(1):276-285. Epub 2019 Dec 17.

College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China.

As emerging alternatives of legacy perfluoroalkyl substances, perfluorophosphinates (PFPiAs) and perfluorophosphonates (PFPAs) are widely applied in industrial and agricultural fields and are supposed to be large partitioned to soil and highly persistent. It is of particular interest to understand their transfer from roots to shoots and transformation in plants, such as wheat. The results of hydroponic experiments indicated that C6/C6 PFPiA, C8/C8 PFPiA, perfluorooctanophosphonic acid (PFOPA), and perfluorohexaphosphonic acid (PFHxPA) were quickly adsorbed on the epidermis of wheat root ( L.), which was driven by their hydrophobicity. A small fraction of the accumulated PFPiAs and PFPAs in the wheat root was subjected to absorption via an active process dependent on H-ATPase. PFHxPA, which has the smallest molecular weight and medium hydrophilicity (log  < 4), displayed the strongest absorption efficiency via the water and anion channels and had the highest translocation potential from roots to shoots in wheat. C6/C6 and C8/C8 PFPiAs experienced phase I metabolism in wheat, although at a low rate, to form more persistent PFHxPA and PFOPA, respectively, as well as 1-perfluorohexane (1-PFHx) and 1-perfluorooctane (1-PFO), which were regulated by cytochrome P450 in wheat root. As a result, exposure to PFPiAs in roots ultimately caused the accumulation of more persistent PFPAs in the above-ground parts of plants, raising concerns on their potential risks on human health.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.est.9b05656DOI Listing
January 2020

Magnetically Powered Shape-Transformable Liquid Metal Micromotors.

Small 2019 12 29;15(52):e1905446. Epub 2019 Nov 29.

Hubei Engineering Research Center of Weak Magnetic-Field Detection and College of Science, China Three Gorges University, Yichang, Hubei, 443002, China.

Shape-transformable liquid metal (LM) micromachines have attracted the attention of the scientific community over the past 5 years, but the inconvenience of transfer routes and the use of corrosive fuels have limited their potential applications. In this work, a shape-transformable LM micromotor that is fabricated by a simple, versatile ice-assisted transfer printing method is demonstrated, in which an ice layer is employed as a "sacrificial" substrate that can enable the direct transfer of LM micromotors to arbitrary target substrates conveniently. The resulting LM microswimmers display efficient propulsion of over 60 µm s (≈3 bodylength s ) under elliptically polarized magnetic fields, comparable to that of the common magnetic micro/nanomotors with rigid bodies. Moreover, these LM micromotors can undergo dramatic morphological transformation in an aqueous environment under the irradiation of an alternating magnetic field. The ability to transform the shape and efficiently propel LM microswimmers holds great promise for chemical sensing, controlled cargo transport, materials science, and even artificial intelligence in ways that are not possible with rigid-bodies microrobots.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/smll.201905446DOI Listing
December 2019

Alkaloids from Traditional Chinese Medicine against hepatocellular carcinoma.

Biomed Pharmacother 2019 Dec 23;120:109543. Epub 2019 Oct 23.

Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China. Electronic address:

Hepatocellular carcinoma (HCC) has become one of the major diseases that are threatening human health in the 21st century. Currently there are many approaches to treat liver cancer, but each has its own advantages and disadvantages. Among various methods of treating liver cancer, natural medicine treatment has achieved promising results because of their superiorities of high efficiency and availability, as well as low side effects. Alkaloids, as a class of natural ingredients derived from traditional Chinese medicines, have previously been shown to exert prominent anti-hepatocarcinogenic effects, through various mechanisms including inhibition of proliferation, metastasis and angiogenesis, changing cell morphology, promoting apoptosis and autophagy, triggering cell cycle arrest, regulating various cancer-related genes as well as pathways and so on. As a consequence, alkaloids suppress the development and progression of liver cancer. In this study, the mechanisms of representative alkaloids against hepatocarcinoma in each class are described systematically according to the structure classification, which mainly divides alkaloids into piperidine alkaloids, isoquinoline alkaloids, indole alkaloids, terpenoids alkaloids, steroidal alkaloids and other alkaloids. Besides using them alone, synergistic effects created together with other chemotherapy drugs and some special preparation methods also have been demonstrated. In this review, we have summarized the potential roles of several common alkaloids in the prevention and treatment of HCC, by revising the preclinical studies, highlighting the potential applications of alkaloids when they function as a therapeutic choice for HCC treatment, and integrating them into clinical practices.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biopha.2019.109543DOI Listing
December 2019

Long Noncoding RNA GMAN, Up-regulated in Gastric Cancer Tissues, Is Associated With Metastasis in Patients and Promotes Translation of Ephrin A1 by Competitively Binding GMAN-AS.

Gastroenterology 2019 02 13;156(3):676-691.e11. Epub 2018 Nov 13.

Department of Cell Biology and Cancer Institute of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China; Institute of Gastroenterology, Zhejiang University, Hangzhou, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, Zhejiang, China; Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada. Electronic address:

Background & Aims: We aimed to identify long noncoding RNAs (lncRNAs) that are up-regulated in gastric cancer tissues from patients and study their function in gastric tumor metastasis.

Methods: We collected gastric tumor and nontumor tissues from patients in China and analyzed levels of lncRNAs by microarray analysis, proteins by immunohistochemistry, and RNAs by quantitative reverse-transcription polymerase chain reaction; we compared these with survival times of patients and tumor progression. RNA levels were knocked down or knocked out in BGC-823, SGC-7901, and MKN45 cell lines using small interfering or short hairpin RNAs or clustered regularly interspaced short palindromic repeats (ie, CRISPR)/CRISPR associated protein 9 (ie, Cas9) vectors. Genes were overexpressed from transfected plasmids in HGC-27 cells. Cells were analyzed by Northern blot and immunoblot, polysome profiling assay, and cell invasion assay. Cells were injected into the tail veins or spleens of nude mice or SCID mice; lung and liver tissues were collected, and metastases were counted. lncRNAs were cloned by using rapid amplification of complementary DNA ends. Their interactions with other genes were determined by RNA pulldown and mapping assays.

Results: In microarray analyses, we identified 151 lncRNAs expressed at significantly higher levels in gastric tumor vs nontumor tissues. Levels of an lncRNA that we called gastric cancer metastasis associated long noncoding RNA (GMAN) were increased in gastric tumor tissues, compared with nontumor tissues; its up-regulation was associated with tumor metastasis and shorter survival times of patients. The GMAN gene overlaps with the ephrin A1 gene (EFNA1) and was highly expressed in BGC-823 and MKN45 cells. Knockdown of GMAN in these cells did not affect proliferation, colony formation, or adhesion but did reduce their invasive activity in Transwell assays. Ectopic expression of GMAN increased the invasive activity of HGC-27 cells. BGC-823 and MKN45 cells with knockdown of GMAN formed fewer metastases after injection into tail veins of nude mice. Knockdown or knockout of GMAN also reduced levels of ephrin A1 protein in cells. We found that GMAN promoted translation of ephrin A1 messenger RNA into protein by binding to the antisense GMAN RNA (GMAN-AS)-this antisense sequence is also complementary to that of ephrin A1 mRNA. Levels of ephrin A1 protein were also increased in gastric tumors from patients with metastases than in those without metastases. Knockout of ephrin A1 in BGC-823 cells reduced their invasive activity in Transwell assays and ability to form metastases after injection into SCID mice. Ectopic expression of ephrin A1 in BGC-823 cells with knockdown or knockout of GMAN restored their invasive activities and ability form metastases in nude or SCID mice. A CRISPR/Cas9-based strategy to disrupt the GMAN gene significantly reduced the numbers of metastases formed from SGC-7901 cells in mice.

Conclusions: We identified an lncRNA, which we call GMAN, that is increased in gastric tumors from patients and associated with survival and formation of metastases. It regulates translation of ephrin A1 mRNA by binding competitively to GMAN-AS. Knockdown or knockout of GMAN or ephrin A1 in gastric cancer cell lines reduces their invasive activity and ability to form metastases after injection into mice. These genes might be targeted to prevent or reduce gastric cancer metastasis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1053/j.gastro.2018.10.054DOI Listing
February 2019

Magnetically Powered Annelid-Worm-Like Microswimmers.

Small 2018 04 3;14(17):e1704546. Epub 2018 Apr 3.

Hubei Engineering Research Center of Weak Magnetic-field Detection & College of Science, China Three Gorges University, Yichang, Hubei, 443002, China.

A bioinspired magnetically powered microswimmer is designed and experimentally demonstrated by mimicking the morphology of annelid worms. The structural parameters of the microswimmer, such as the surface wrinkling, can be controlled by applying prestrain on substrate for the precise fabrication and consistent performance of the microswimmers. The resulting annelid-worm-like microswimmers display efficient propulsion under an oscillating magnetic field, reaching a peak speed of ≈100 µm s . The speed and directionality of the microswimmer can be readily controlled by changing the parameters of the field inputs. Additionally, it is demonstrated that the microswimmers are able to transport microparticles toward a predefined destination, although the translation velocity is inevitably reduced due to the additional hydrodynamic resistance of the microparticles. These annelid-worm-like microswimmers have excellent mobility, good maneuverability, and strong transport capacity, and they hold considerable promise for diverse biomedical, chemical sensing, and environmental applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/smll.201704546DOI Listing
April 2018

Magnetically Actuated Wormlike Nanomotors for Controlled Cargo Release.

ACS Appl Mater Interfaces 2015 Dec 18;7(47):26017-21. Epub 2015 Nov 18.

College of Science, China Three Gorges University , Yichang 443002, Hubei, China.

Magnetically actuated nanomotor, which swims under externally applied magnetic fields, shows great promise for controlled cargo delivery and release in biological fluids. Here, we report an on-demand release of 6-carboxyfluoresceins (FAM), a green fluorescein, from G-quadruplex DNA functionalized magnetically actuated wormlike nanomotors by applying an alternating magnetic field. This field-triggered FAM releasing process can be easily controlled by multiple parameters such as magnetic field, frequency, and exposure time. In addition, the experimental results and the theoretical simulation demonstrate that both a thermal and a nonthermal mechanism are involved in the cargo releasing process.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.5b08946DOI Listing
December 2015

A SERS/fluorescence dual-mode nanosensor based on the human telomeric G-quadruplex DNA: Application to mercury (II) detection.

Biosens Bioelectron 2015 Jul 7;69:142-7. Epub 2015 Feb 7.

College of Science, China Three Gorges University, Yichang 443002, Hubei, China. Electronic address:

DNA-metal nanoparticle conjugates have been increasingly exploited for sensing purposes over the past decades. However, most of the existing strategies are operated with canonical DNA structures, such as single-stranded forms, stem-loop structures, and double helix structures. There is intense interest in the development of nano-system based on high order DNA secondary structures. Herein, we propose a SERS/fluorescence dual-mode nanosensor, where the signal transduction mechanism is based on the conformational switching of the human telomeric G-quadruplex DNA. The nanosensor exhibits excellent SERS/fluorescence responses to the complementary strands of G-quadruplexes. Based on T-Hg(2+)-T coordination chemistry, this sensor is effectively applied to determination of Hg(2+) in buffer solution and real samples. It achieves a limit of detection (LOD) as low as 1ppt, which is ~100 times more sensitive than conventional optical sensors. We anticipate that the proposed G-quadruplex-based nanosensor could be applied to the analysis of other metal ions and small molecules in environmental samples and biological systems.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bios.2015.02.009DOI Listing
July 2015

Magnetic control of valley and spin degrees of freedom via magnetotransport in n-type monolayer MoS2.

J Phys Condens Matter 2014 Dec 6;26(48):485008. Epub 2014 Nov 6.

Department of Physics and Key Laboratory for Low-Dimensional Structures and Quantum Manipulation (Ministry of Education), Hunan Normal University, Changsha 410081, People's Republic of China.

We study the quantum magnetotransport properties at low temperature for n-type monolayer MoS2 under a perpendicular magnetic field within the linear response theory. By using Kubo formula, it is predicted that the valley- and spin-resolved Hall conductance obeys the same rule as that for conventional fermions. However, the first Hall plateau and the longitudinal charge conductance in low energies are fully valley/spin-polarized, which may produce a pure valley/spin current. In the low magnetic field limit, importantly, the resistance as a function of field is in good agreement with the available experiment data. These interesting findings can be tested experimentally and may be useful for valleytronic and spintronic applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1088/0953-8984/26/48/485008DOI Listing
December 2014

Snail-regulated MiR-375 inhibits migration and invasion of gastric cancer cells by targeting JAK2.

PLoS One 2014 23;9(7):e99516. Epub 2014 Jul 23.

Department of Cell Biology and Program in Molecular cell Biology, Zhejiang University School of Medicine, Hangzhou, China; Institute of Gastroenterology, Zhejiang University, Hangzhou, China.

MicroRNAs (miRNAs) have been reported to play a critical role in cancer invasion and metastasis. Our previous study showed that miR-375 frequently downregulated in gastric cancer suppresses cell proliferation by targeting Janus kinase 2 (JAK2). Here, we further found that the expression level of miR-375 is significantly decreased in metastatic gastric cancer tissues compared with the non-metastasis controls. Ectopic expression of miR-375 inhibits the migration and invasion of gastric cancer cells partially by targeting JAK2. Furthermore, miR-375 expression is negatively regulated by the metastasis associated transcription factor Snail, which directly binds to the putative promoter of miR-375. Moreover, overexpression of Snail can partially reverse the inhibition of gastric cancer cell migration caused by miR-375. Taken together, these data suggest that miR-375 may be negatively regulated by Snail and involved in gastric cancer cell migration and invasion potentially by targeting JAK2.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0099516PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4108470PMC
November 2015

The sacred lotus genome provides insights into the evolution of flowering plants.

Plant J 2013 Nov 11;76(4):557-67. Epub 2013 Oct 11.

Wuhan Vegetable Research Institute, Wuhan, 430065, China.

Sacred lotus (Nelumbo nucifera) is an ornamental plant that is also used for food and medicine. This basal eudicot species is especially important from an evolutionary perspective, as it occupies a critical phylogenetic position in flowering plants. Here we report the draft genome of a wild strain of sacred lotus. The assembled genome is 792 Mb, which is approximately 85-90% of genome size estimates. We annotated 392 Mb of repeat sequences and 36,385 protein-coding genes within the genome. Using these sequence data, we constructed a phylogenetic tree and confirmed the basal location of sacred lotus within eudicots. Importantly, we found evidence for a relatively recent whole-genome duplication event; any indication of the ancient paleo-hexaploid event was, however, absent. Genomic analysis revealed evidence of positive selection within 28 embryo-defective genes and one annexin gene that may be related to the long-term viability of sacred lotus seed. We also identified a significant expansion of starch synthase genes, which probably elevated starch levels within the rhizome of sacred lotus. Sequencing this strain of sacred lotus thus provided important insights into the evolution of flowering plant and revealed genetic mechanisms that influence seed dormancy and starch synthesis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/tpj.12313DOI Listing
November 2013

Spatial distribution of spin polarization in a channel on the surface of a topological insulator.

J Phys Condens Matter 2012 May 5;24(18):185301. Epub 2012 Apr 5.

Department of Physics and Key Laboratory for Low-Dimensional Quantum Structures and Manipulation (Ministry of Education), Hunan Normal University, Changsha 410081, People's Republic of China.

We study the spatial distribution of electron spin polarization for a gate-controlled T-shaped channel on the surface of a three-dimensional topological insulator (3D TI). We demonstrate that an energy gap depending on channel geometry parameters is definitely opened due to the spatial confinement. Spin surface locking in momentum space for a uniform wide channel with Hamiltonian linearity in the wavevector is still kept, but it is broken with Hamiltonian nonlinearity in the wavevector, like that for two-dimensional surface states widely studied in the literature. However, the spin surface locking for a T-shaped channel is broken even with Hamiltonian linearity in the wavevector. Interestingly, the magnitude and direction of the in-plane spin polarization are spatially dependent in all regions due to the breaking of translational symmetry of the T-shaped channel system. These interesting findings for an electrically controlled nanostructure based on the 3D TI surface may be testable with the present experimental technique, and may provide further understanding the nature of 3D TI surface states.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1088/0953-8984/24/18/185301DOI Listing
May 2012

Sixteen polymorphic microsatellite markers from Zizania latifolia Turcz. (Poaceae).

Mol Ecol Resour 2009 May 3;9(3):887-9. Epub 2009 Feb 3.

Key Laboratory of MOE for Plant Development Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China Wuhan National Field Observation & Research Station for Aquatic Vegetables, Wuhan 430065, China.

Sixteen polymorphic microsatellite markers were isolated and identified in Zizania latifolia Turcz. (Poaceae), a perennial aquatic plant widespread in Eastern Asia. The microsatellite-enriched library was constructed using the fast isolation by AFLP of sequences containing repeats method. These markers revealed two to 14 alleles, with an average of 5.6 alleles per locus. The observed and expected heterozygosities varied from 0.071 to 0.690 and from 0.174 to 0.812, respectively. These markers will be useful for studying of gene flow and evaluating the genetic diversity of the Zizania latifolia population.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1755-0998.2008.02357.xDOI Listing
May 2009