Publications by authors named "Yilin Zhao"

138 Publications

Unfractionated Heparin Attenuated Histone-Induced Pulmonary Syndecan-1 Degradation in Mice: a Preliminary Study on the Roles of Heparinase Pathway.

Inflammation 2021 Oct 16. Epub 2021 Oct 16.

Department of Critical Care Medicine, the First Affiliated Hospital, China Medical University, North Nanjing Street 155, Shenyang, 110001, Liaoning Province, People's Republic of China.

Endothelial glycocalyx degradation is thought to facilitate the development of sepsis. Histone is a significant mediator in sepsis. Unfractionated heparin (UFH) possessed beneficial effects on sepsis. Thereby, this study aims to figure out whether histone can disrupt glycocalyx and to investigate the protective effect and mechanism of UFH. Male mice (C57BL/6, 8-10 weeks old, weighing 20-25 g) were randomly divided into five groups including control group, histone group, histone + UFH group, histone + heparinase (HPA) inhibitor group, and histone + UFH + HPA inhibitor group. The mice were treated with histone (50 mg/kg) via tail vein immediately after HPA (20 mg/kg) injection. UFH (400 U/kg) was injected 1h after histone administration. The other groups were injected with equal volume of sterile saline accordingly. UFH alleviated histone-induced lung injury and pulmonary edema. UFH inhibited histone-induced lung coagulation activation and inflammatory response. UFH treatment markedly inhibited pulmonary glycocalyx degradation by reducing the histone-induced decrease in the levels of lung syndecan-1 mRNA and protein. UFH downregulated histone-induced expression of HPA mRNA and protein, and thus alleviated glycocalyx degradation. UFH protects against histone-induced pulmonary glycocalyx injury partly by heparinase pathway.
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http://dx.doi.org/10.1007/s10753-021-01578-wDOI Listing
October 2021

The favorable prognosis of cystic biliary atresia may be related to early surgery and mild liver pathological changes.

Pediatr Surg Int 2021 Oct 7. Epub 2021 Oct 7.

Department of General Surgery, Tianjin Children's Hospital, LongYan Road 238, Beichen District, Tianjin, 300134, People's Republic of China.

Objective: The objectives of this study is to compare the prognostic differences between cystic biliary atresia (CBA) and non-CBA, analyze the clinical and liver pathological differences between the two groups, and explore the possible factors that affect the native liver survival of infants with CBA after Kasai portoenterostomy (KPE).

Methods: From 2013 to 2020, 131 infants with BA were admitted to Tianjin Children's Hospital. A total of 108 infants with BA were included after excluding those who did not undergo surgery after diagnosis (n = 23), including 12 cases of CBA and 96 cases of non-CBA. The clinical data, native liver survival and liver pathology, including liver fibrosis, bile ductular proliferation (BDP), bile plug, and portal area inflammation infiltration of the two study groups were compared.

Results: CBA accounts for 9.16% (12/131) and type I CBA accounts for 6.87% (9/131) of all types of BA. 16.7% (2/12) of CBA were detected prenatally with diagnosis of choledochal cyst (CC). The age at KPE, total bilirubin, direct bilirubin, and total bile acid levels of CBA were significantly lower than those of non-CBA (P = 0.047, P = 0.013, P = 0.009, P = 0.010, respectively). The long and wide diameters of the gallbladder were significantly larger than those of non-CBA (both P < 0.001). The 1-, 3-, and 5-year survival rates of CBA were 83.3%, 71.4%, and 71.4%, respectively, and 56.5%, 32.5%, and 29.8%, respectively, in non-CBA. The difference between the two groups was statistically significant (P = 0.031). The degree of liver fibrosis and bile plug in non-CBA was higher than that of CBA (P = 0.004, P < 0.001, respectively). There was no difference of BDP and inflammation infiltration between the two groups (P = 0.285, P = 0.243, respectively).

Conclusion: CBA is a distinct type different from non-CBA, with different pathological processes, pathological manifestations, and clinical prognosis. The favorable prognosis of CBA may be derived from the early diagnosis, early operation, and mild pathological changes.
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http://dx.doi.org/10.1007/s00383-021-05030-wDOI Listing
October 2021

Targeting epigenetically maladapted vascular niche alleviates liver fibrosis in nonalcoholic steatohepatitis.

Sci Transl Med 2021 Oct 6;13(614):eabd1206. Epub 2021 Oct 6.

Key Laboratory of Birth Defects and Related Diseases of Women and Children of MOE, State Key Laboratory of Biotherapy, West China Second University Hospital, Sichuan University, Chengdu 610041, China.

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http://dx.doi.org/10.1126/scitranslmed.abd1206DOI Listing
October 2021

Rapid colonization and biodegradation of untreated commercial polyethylene wrap by a new strain of Bacillus velezensis C5.

J Environ Manage 2021 Sep 28;301:113848. Epub 2021 Sep 28.

Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, North Third Ring Road 15, Chaoyang District, Beijing, 100029, PR China. Electronic address:

Biodegradation could be a potential alternative solution to polyethylene (PE) pollution. However, its hydrophobic surface and long carbon chains make extremely low biodegradation efficiency. In this study, we screened a novel potential bacterial strain C5 (CGMCC number: 1.18715) for low-density polyethylene (LDPE) biodegrading from landfills. The strain was identified as Bacillus velezensis according to its 16S rRNA sequence. The contact angle analysis indicated that C5 could rapidly form biofilm on untreated LDPE which resulted in contact angles decreasing from 100° to 54° over 7 d. After the LDPE film incubated with C5 for 90 d, the thickness and weight of LDPE film decreased by 26% and 8.01%, respectively. Besides, the biotreated PE film was found with increases in weight-averaged molecular weight by 29.8%, suggesting low molar mass chains were consumed. C-C n-alkanes were detected in the biodegradation products, which proved the depolymerization of LDPE. Combined with the genome mining results, a possible biofilm-aided degrading mechanism was proposed and might involve key enzymes, such as laccase, cytochrome P450 and propionyl-CoA carboxylase, which could constitute a multienzyme system for the co-catalytic degradation of LDPE waste.
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http://dx.doi.org/10.1016/j.jenvman.2021.113848DOI Listing
September 2021

Cationic polyacrylamide alleviated the inhibitory impact of ZnO nanoparticles on anaerobic digestion of waste activated sludge through reducing reactive oxygen species induced.

Water Res 2021 Oct 9;205:117651. Epub 2021 Sep 9.

College of Environmental Science and Engineering, Hunan University and Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.

The enrichment of zinc oxide nanoparticles (ZnO NPs) in waste activated sludge (WAS) has raised concerns about their potential impact on anaerobic digestion of WAS. To date, there is no information regarding how to attenuate the negative effects of ZnO NPs on WAS anaerobic digestion. In this study, it was found that the appropriate amount of cationic polyacrylamide (cPAM) could mitigate the toxicity of ZnO NPs. During short-term exposure, the supplement of 4.0 mg cPAM/g TSS significantly restored biochemical methane potential from 28.6% inhibition to 9.3% inhibition compared with the control digester (P < 0.01). The spiked cPAM promoted the solubilization and acidification stages by weakening the contact between ZnO NPs and anaerobes in anaerobic digestion process, thus providing abundant substance for sequent bio-utilization. In the long-term semi-continues operated reactor, the continuous replacement of cPAM (at 4.0 mg/g TSS) significantly strengthened the recovery of VS destruction rate (20.3% to 26.4%, P < 0.01) and the daily yield of methane (93.5 mL/d to 124.2 mL/d, P < 0.01). Consistent with the restored performance, the application of cPAM increased the total microbial communities and the relative abundances of dominant acidogens and methanogens. Further explorations showed decreased toxicity of ZnO NPs primarily attributed to the decline of reactive oxygen species (ROS) induced by ZnO NPs.
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http://dx.doi.org/10.1016/j.watres.2021.117651DOI Listing
October 2021

CHANGES IN THE LEVELS OF THEILERIA ORIENTALIS IKEDA TYPE INFECTION IN HAEMAPHYSALIS LONGICORNIS NYMPHS OVER A SIX-MONTH PERIOD.

J Parasitol 2021 Sep;107(5):710-716

School of Agriculture and Environment, Massey University, Palmerston North, 4442, New Zealand.

This study aimed to investigate whether the infection intensity of Theileria orientalis Ikeda type organisms within Haemaphysalis longicornis larvae and nymph stages fluctuated over 6 mo after feeding as larvae on infected calves in the field. Naïve larvae, hatched from eggs, were fed on infected calves for 5 days while contained within cotton socks glued over the calves' ears. Larvae were first sampled immediately post-feeding and then sampled every 3 wk for 23 wk in total, after molting to nymphs. All larvae and nymphs were tested for T. orientalis Ikeda organisms using quantitative PCR. The qPCR results showed that the infection intensity of Haemaphysalis longicornis larvae and nymphs was not constant over the sampling period, and after initially dropping after molting to nymphs, it then rose with fasting to a maximum at 17 and 23 wk post-feeding. The significant rise in T. orientalis Ikeda organisms observed at 23 wk postfeeding may explain why more severe clinical cases of bovine theileriosis in New Zealand are seen in the spring when nymphs are the predominant instar questing.
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http://dx.doi.org/10.1645/20-177DOI Listing
September 2021

RNA Sequence Profiling Reveals Unique Immune and Metabolic Features of Breast Cancer Brain Metastases.

Front Oncol 2021 26;11:679262. Epub 2021 Aug 26.

Department of Oncology and Vascular Interventional Radiology, Zhongshan Hospital, Xiamen University, Xiamen, China.

There is an urgent need to improve our understanding of breast cancer brain metastases (BCBMs). Thus, we obtained transcriptome data of BCBMs, primary breast cancers (BCs), and extracranial metastases (BCEMs) from the Gene Expression Omnibus (GEO) database, including GSE43837, GSE14017, and GSE14018, for immune and metabolic analysis. Firstly, we performed immune and metabolic analysis on BCBMs and primary breast cancers of GSE43837 using RNA sequence. We identified significant immunosuppression and gene signatures associated with immune infiltration in BCBMs; the lower the expression of the signatures, the worse the prognosis of breast cancer patients in the Kaplan-Meier (KM) plotter [Breast cancer] database. We also identified increased oxidative phosphorylation (OXPHOS) utilization in BCBMs compared with BCs and gene signatures associated with increased OXPHOS utilization in BCBMs; the higher the expression of the signatures, the worse the prognosis of breast cancer patients in the KM plotter [Breast cancer] database, which can predict the prognosis of breast cancer patients better, as it can also predict the prognosis of patients with different breast cancer subtypes. In addition, we performed immune and metabolic analysis on BCBMs and extracranial metastases of GSE14017 and GSE14018 using RNA sequence. Compared with extracranial metastases, we identified more significant immunosuppression but no difference in OXPHOS utilization in BCBMs, which may be because OXPHOS was also involved in extracranial metastases. We have proven that OXPHOS was functionally significant in metastasis assays. Oligomycin, an OXPHOS inhibitor, substantially attenuated the migration and invasion potential of breast cancer cells. Our study provides new insights into the pathogenesis of BCBMs.

Significance: Our study reports the most comprehensive gene expression analysis of BCBMs, BCs and extracranial metastases to date. We identified immunosuppression and OXPHOS enrichment in BCBMs compared with BCs, which provide new insights into the pathogenesis of BCBMs and will facilitate the development of new therapeutic strategies for patients with BCBMs.
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http://dx.doi.org/10.3389/fonc.2021.679262DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8427193PMC
August 2021

Intensive glucose control during the perioperative period for diabetic patients undergoing surgery: An updated systematic review and meta-analysis.

J Clin Anesth 2021 Sep 9;75:110504. Epub 2021 Sep 9.

Department of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. Electronic address:

Study Objective: To evaluate the impact of intensive glucose control on diabetic patients undergoing surgery.

Design: A systematic review and meta-analysis of randomized controlled trials. PubMed, CENTRAL, EMBASE, ISI Web of Science, and CINAHL databases were searched from inception to 13 December 2020.

Setting: Operating room, postoperative recovery area and ward, up to 30 days after surgery.

Patients: Diabetic patients undergoing surgery.

Interventions: We used Review Manager 5.4 to pool the data with a random-effects model. The quality of evidence was rated using the Grading of Recommendations, Assessment, Development and Evaluation system.

Measurements: The primary outcomes were infectious complications, postoperative mortality, and hypoglycaemia. The secondary outcomes included atrial fibrillation, myocardial infarction, stroke, delirium, renal failure, postoperative mechanical ventilation time, length of intensive care unit (ICU) stay, and hospital stay.

Main Results: Thirteen studies involving 1582 participants were included. Compared with conventional glucose control, intensive glucose control was associated with a lower risk of infectious complications (risk ratio [RR], 0.35; 95% confidence interval [CI], 0.19-0.63; low-quality evidence), atrial fibrillation (RR, 0.55; 95% CI, 0.42-0.71; high-quality evidence), and renal failure (RR, 0.38; 95% CI, 0.15-0.95; moderate-quality evidence), as well as a shorter length of stay in the ICU (mean difference (MD), -0.55 day; 95% CI, -1.05 to -0.05 days; very-low-quality evidence) and hospital (MD, -1.61 days; 95% CI, -2.78 to -0.44 days; very-low-quality evidence). However, intensive glucose control was associated with a higher risk of hypoglycaemia (RR, 3.00; 95% CI, 1.97-4.55; high-quality evidence). There were no significant differences in postoperative mortality, myocardial infarction, stroke, delirium, or postoperative mechanical ventilation time.

Conclusions: Intensive glucose control in diabetic patients is associated with a reduction in some adverse postoperative outcomes including infectious complications, but also appears to increase the risk of hypoglycaemia. Further well-designed studies may be needed to determine appropriate regimens to reduce hypoglycaemia incidence.

Prospero Registration Number: CRD42021226138.
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http://dx.doi.org/10.1016/j.jclinane.2021.110504DOI Listing
September 2021

HO Self-Supplying and GSH-Depleting Nanoplatform for Chemodynamic Therapy Synergetic Photothermal/Chemotherapy.

ACS Appl Mater Interfaces 2021 Sep 9;13(37):43925-43936. Epub 2021 Sep 9.

Fujian Provincial Key Laboratory of Innovative Drug Target Research, School of Pharmaceutical Sciences, Xiamen University, Xiamen 361102, P. R. China.

Chemodynamic therapy (CDT) that utilizes Fenton-type reactions to convert endogenous hydrogen peroxide (HO) into hydroxyl radicals (OH) is a promising strategy in anticancer treatment, but the overexpression of glutathione (GSH) and limited endogenous HO make the efficiency of CDT unsatisfactory. Here, an intelligent nanoplatform [email protected]/DOX-HA (CPPDH), which induced the depletion of GSH and the self-supply of HO, was proposed. When CPPDH entered tumor cells through the targeting effect of hyaluronic acid (HA), a release of Cu and produced HO were triggered by the acidic environment of lysosomes. Then, the Cu was reduced by GSH to Cu, and the Cu catalyzed HO to produce OH. The generation of OH could be distinctly enhanced by the GSH depletion and HO self-sufficiency. Besides, an outstanding photothermal therapy (PTT) effect could be stimulated by NIR irradiation on mesoporous polydopamine (mPDA). Meanwhile, mPDA was an excellent photoacoustic reagent, which could monitor the delivery of nanocomposite materials through photoacoustic (PA) imaging. Moreover, the successful delivery of doxorubicin (DOX) realized the integration of chemotherapy, PTT, and CDT. This strategy could solve the problem of insufficient CDT efficacy caused by the limited HO and overexpression of GSH. This multifunctional nanoplatform may open a broad path for self-boosting CDT and synergistic therapy.
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http://dx.doi.org/10.1021/acsami.1c10341DOI Listing
September 2021

Prognosis of Biliary Atresia Associated With Cytomegalovirus: A Meta-Analysis.

Front Pediatr 2021 18;9:710450. Epub 2021 Aug 18.

Department of General Surgery, Tianjin Children's Hospital, Tianjin, China.

The etiology of biliary atresia is unclear, but viral infection has been implicated. The aim of the current meta-analysis was to investigate relationships between cytomegalovirus (CMV) and the prognosis of biliary atresia. PubMed, Embase, the Cochrane Library, the China National Knowledge Infrastructure database, and Wanfang Data electronic databases were searched for eligible studies. Each relevant text was thoroughly reviewed and examined, including related papers in their reference lists. A total of nine studies including 784 patients were included in the analysis. Biliary atresia patients with CMV exhibited significantly lower jaundice clearance (odds ratio: 0.46, < 0.0001; = 15%, = 0.31). There were no significant differences in the rates of cholangitis or native liver survival. CMV-pp65-positive biliary atresia patients had a significantly lower rate of jaundice clearance (odds ratio: 5.87, = 0.003; = 0%, = 0.71) and a significantly higher rate of cholangitis (odds ratio: 0.21, = 0.01; = 0%, = 0.43) than CMV antibody-positive biliary atresia patients. Biliary atresia patients who were also infected with CMV had a poorer prognosis, particularly with respect to jaundice clearance. CMV status may influence the prognosis of biliary atresia. Clinicians should be able to routinely identify the subset of biliary atresia patients who are also CMV-positive, in order to improve native liver survival.
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http://dx.doi.org/10.3389/fped.2021.710450DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8416545PMC
August 2021

Zirconia supported gold-palladium nanocatalyst for NAD(P)H regeneration via two-step mechanism.

Nanotechnology 2021 Sep 6;32(48). Epub 2021 Sep 6.

Beijing Key Laboratory of Bioprocess, Beijing Advanced Innovation Center for Soft Matter Science and Engineering (BAIC-SM), College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, People's Republic of China.

The regeneration cycle of expensive cofactor, NAD(P)H, is of paramount importance for the bio-catalyzed redox reactions. Here a ZrOsupported bimetallic nanocatalyst of gold-palladium (Au-Pd/ZrO) was prepared to catalyze the regeneration of NAD(P)H without using electron mediators and extra energy input. Over 98% of regeneration efficiency can be achieved catlyzed by Au-Pd/ZrOusing TEOA as the electron donor. Mechanism study showed that the regeneration of NAD(P)H took place through a two-step process: Au-Pd/ZrOnanocatalyst first catalyzed the oxidation of triethanolamine (TEOA) to glycolaldehyde (GA), then the generated GA induced the non-catalytic reducing of NAD(P)to NAD(P)H under an alkaline environment maintained by TEOA. This two-step mechanism enables the decoupling of the regeneration of NAD(P)H in space and time into a catalytic oxidation and non-catalytic reducing cascade process which has been further verified using a variety of electron donors. The application significance of this procedure is further demonstrated both by the favorable stability of Au-Pd/ZrOnanocatalyst in 5 successive cycles preserving over 90% of its original activity, and by the excellent performance of the regenerated NADH as the cofactor in the catalytic hydrogenation of acetaldehyde using an ethanol dehydrogenase.
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http://dx.doi.org/10.1088/1361-6528/ac1e51DOI Listing
September 2021

Detecting Pb2+ by a "turn-on" Fluorescence Sensor based on DNA Functionalized magnetic Nanocomposites.

Nanotechnology 2021 Aug 16. Epub 2021 Aug 16.

Beijing University of Chemical Technology, No.15, Beisanhuandong Road, Chaoyang District, Beijing, China, Beijing, 100029, CHINA.

Sensitive and selective detection of the lead ion (Pb) plays an important role in terms of both human health and environmental protection, as the heavy metal is fairly ubiquitous and highly toxic. The highly stable fluorescence biosensor is composed of [email protected] nanoflowers, functionalized with a carboxyl fluorescein labeled DNA. The morphology, physical and chemical properties of the sensing nanomaterials were studied by Transmission Electron Microscopy (TEM), FT-IR spectroscopy (FT-IR), X-ray powder diffraction (XRD) and vibrating sample magnetometer (VSM). UV-visible and fluorescence spectroscopy were used to characterize the fluorescein functionalized magnetic nanoparticles. The performance of Pb Pbdetection displayed an excellent linearity (R=0.9948) in the range of 10to 5×10ppm with a detection limit of 10ppm, based on the optimization of the fabrication process and aptamers' specification. The fluorescence biosensor has a very fast response, excellent recoveries and high adsorbent capacities. It was successfully applied for the determination of Pb Pbin contaminated water and serum samples; the detection of limit (LOD) in both media were 10ppm. These features ensure the potential use of aptamer functionalized magnetic nanoflowers as a new class of non-toxic biocompatible sensors for biological and environmental applications.
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http://dx.doi.org/10.1088/1361-6528/ac1dd3DOI Listing
August 2021

The Role of Perioperative Sleep Disturbance in Postoperative Neurocognitive Disorders.

Nat Sci Sleep 2021 6;13:1395-1410. Epub 2021 Aug 6.

Department of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei, People's Republic of China.

Postoperative neurocognitive disorder (PND) increases the length of hospital stay, mortality, and risk of long-term cognitive impairment. Perioperative sleep disturbance is prevalent and commonly ignored and may increase the risk of PND. However, the role of perioperative sleep disturbances in PND remains unclear. Nocturnal sleep plays an indispensable role in learning, memory, and maintenance of cerebral microenvironmental homeostasis. Hospitalized sleep disturbances also increase the incidence of postoperative delirium and cognitive dysfunction. This review summarizes the role of perioperative sleep disturbances in PND and elucidates the potential mechanisms underlying sleep-deprivation-mediated PND. Activated neuroinflammation and oxidative stress; impaired function of the blood-brain barrier and glymphatic pathway; decreased hippocampal brain-derived neurotrophic factor, adult neurogenesis, and sirtuin1 expression; and accumulated amyloid-beta proteins are associated with PND in individuals with perioperative sleep disorders. These findings suggest that the improvement of perioperative sleep might reduce the incidence of postoperative delirium and postoperative cognitive dysfunction. Future studies should further investigate the role of perioperative sleep disturbance in PND.
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http://dx.doi.org/10.2147/NSS.S320745DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8354730PMC
August 2021

PDE2 Inhibits PKA-Mediated Phosphorylation of TFAM to Promote Mitochondrial Ca-Induced Colorectal Cancer Growth.

Front Oncol 2021 21;11:663778. Epub 2021 Jun 21.

State Key Laboratory of Cancer Biology and Experimental Teaching Center of Basic Medicine, Fourth Military Medical University, Xi'an, China.

Growing evidence indicates that the dysregulation of mitochondrial calcium (Ca) plays a critical role in the growth of tumor cells, including colorectal cancer (CRC). However, the underling mechanism is not fully elucidated. In this study, the regulatory effects of mitochondrial Ca on phosphodiesterase 2 (PDE2)/cAMP/PKA axis and the phosphorylation of mitochondrial transcription factor A (TFAM) as well as the growth of CRC cells were systematically investigated both and Our findings demonstrated that MCU-induced mitochondrial Ca uptake activated mitochondrial PDE2 in CRC cells. Moreover, overexpression MCU in CRC led to a 1.9-fold increase in Ca uptake compared to control cells. However, knockdown of MCU resulted in 1.5-fould decrease in Ca uptake in mitochondria compared to the controls. Activation of mitochondrial PDE2 significantly inhibited the activity of mitochondrial protein kinase A (PKA), which subsequently leads to decreased phosphorylation of TFAM. Our data further revealed that PKA regulates the phosphorylation of TFAM and promotes the degradation of phosphorylated TFAM. Thus, TFAM protein levels accumulated in mitochondria when the activity of PKA was inhibited. Overall, this study showed that the overexpression of MCU enhanced CRC growth through promoting the accumulation of TFAM proteins in mitochondria. Conversely, knockdown of MCU in CRC cells resulted in decreased CRC growth. Collectively, these data suggest that the mitochondrial Ca-activated PDE2/cAMP/PKA axis plays a key role in regulating TFAM stability and the growth of CRC cells.
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http://dx.doi.org/10.3389/fonc.2021.663778DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256694PMC
June 2021

Mitochondrial transcription factor A plays opposite roles in the initiation and progression of colitis-associated cancer.

Cancer Commun (Lond) 2021 08 23;41(8):695-714. Epub 2021 Jun 23.

State Key Laboratory of Cancer Biology and Department of Physiology and Pathophysiology, Fourth Military Medical University, Xi'an, Shaanxi, 710032, P. R. China.

Background: Mitochondria are key regulators in cell proliferation and apoptosis. Alterations in mitochondrial function are closely associated with inflammation and tumorigenesis. This study aimed to investigate whether mitochondrial transcription factor A (TFAM), a key regulator of mitochondrial DNA transcription and replication, is involved in the initiation and progression of colitis-associated cancer (CAC).

Methods: TFAM expression was examined in tissue samples of inflammatory bowel diseases (IBD) and CAC by immunohistochemistry. Intestinal epithelial cell (IEC)-specific TFAM-knockout mice (TFAM ) and colorectal cancer (CRC) cells with TFAM knockdown or overexpression were used to evaluate the role of TFAM in colitis and the initiation and progression of CAC. The underlying mechanisms of TFAM were also explored by analyzing mitochondrial respiration function and biogenesis.

Results: The expression of TFAM was downregulated in active IBD and negatively associated with the disease activity. The downregulation of TFAM in IECs was induced by interleukin-6 in a signal transducer and activator of transcription 3 (STAT3)/miR-23b-dependent manner. In addition, TFAM knockout impaired IEC turnover to promote dextran sulfate sodium (DSS)-induced colitis in mice. Of note, TFAM knockout increased the susceptibility of mice to azoxymethane/DSS-induced CAC and TFAM overexpression protected mice from intestinal inflammation and colitis-associated tumorigenesis. By contrast, TFAM expression was upregulated in CAC tissues and contributed to cell growth. Furthermore, it was demonstrated that β-catenin induced the upregulation of TFAM through c-Myc in CRC cells. Mechanistically, TFAM promoted the proliferation of both IECs and CRC cells by increasing mitochondrial biogenesis and activity.

Conclusions: TFAM plays a dual role in the initiation and progression of CAC, providing a novel understanding of CAC pathogenesis.
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http://dx.doi.org/10.1002/cac2.12184DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8360642PMC
August 2021

Pyroptosis in the Initiation and Progression of Atherosclerosis.

Front Pharmacol 2021 26;12:652963. Epub 2021 May 26.

Department of Internal Medicine, Affiliated Wuxi Matemity and Child Health Care Hospital of Nanjing Medical University, Wuxi, China.

Pyroptosis, a newly discovered form of programmed cell death, is characterized by cell swelling, the protrusion of large bubbles from the plasma membrane and cell lysis. This death pathway is mediated by the pore formation of gasdermin D (GSDMD), which is activated by human caspase-1/caspase-4/caspase-5 (or mouse caspase-1/caspase11), and followed with the releasing of both cell contents and proinflammatory cytokines. Pyroptosis was initially found to function as an innate immune effector mechanism to facilitate host defense against pathogenic microorganisms, and subsequent studies revealed that pyroptosis also plays an eventful role in inflammatory immune diseases and tumor resistance. Recent studies have also shown that pyroptosis is involved in the initiation, the progression and complications of atherosclerosis. Here, we provide an overview of the role of pyroptosis in atherosclerosis by focusing on three important participating cells: ECs, macrophages, and SMCs. In addition, we also summarized drugs and stimuli that regulate the progression of atherosclerosis by influencing cell pyroptosis.
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http://dx.doi.org/10.3389/fphar.2021.652963DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187899PMC
May 2021

A general approach to realizing perovskite nanocrystals with insulating metal sulfate shells.

Nanoscale 2021 Jun;13(23):10329-10334

Experimental Center of Advanced Materials, School of Materials Science and Engineering, Beijing Institute of Technology, 5 Zhongguancun South Street, Beijing 100081, China.

The strategy of constructing the core/shell structure is of great importance in emitting semiconductor nanocrystals. However, the coating on soft metal halide perovskite nanocrystals at the single particle level remains a challenge because of the low compatibility between perovskites and common wide-band-gap semiconductors, such as ZnS and CdS. In addition, using these semiconductors as the shell layer requires high reaction temperatures, which often lead to undesirable chemical transformation. Herein we report a general route to passivate the perovskite nanocrystals by insulating metal sulfate shells. The passivating shell is created around the as-synthesized CsPbBr3 perovskite nanocrystals by initiating the reaction between an organic ammonium sulfate and a variety of metal ions in the presence of ligands. This new method allowed for creating insulating metal sulfate shells with controllable thicknesses and without unwanted chemical transformation. Importantly, these novel core/shell-structured nanocrystals show photoluminescence quantum yields near unity, highly suppressed energy transfer in film and suppressed halide exchange in solution.
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http://dx.doi.org/10.1039/d1nr01671gDOI Listing
June 2021

Physicochemical properties and microstructure of corn flour-cellulose fiber extrudates.

Food Sci Nutr 2021 May 16;9(5):2497-2507. Epub 2021 Mar 16.

College of Food Science and Engineering National Engineering Laboratory for Wheat and Corn Deep Processing Jilin Agricultural University Changchun China.

In this study, corn flour with 24% w/w moisture content was extruded, and cellulose at varied weight ratios was added in order to investigate its effect on the extrudate's physicochemical properties. Twin-screw extrusion was divided into five temperature zones, and the screw temperature profile was 60℃, 120℃, 140℃, 120℃, and 110℃, respectively, and screw speed was 150 rpm. The cellulose content was 1%-15% w/w. Results showed that the addition of cellulose led to an increase in hardness, L and b of the extruded samples, and a decrease in degree of expansion, a, thermal enthalpy of the sample paste. The sample paste exhibited a solid-like characteristic. Microscopic morphology analysis showed that surface wrinkles of the sample increased with the increase of cellulose addition. The addition of cellulose can effectively increase the degree of puffing of corn flour-cellulose fiber extrudates.
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http://dx.doi.org/10.1002/fsn3.2195DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116859PMC
May 2021

Vagus nerve stimulation in brain diseases: Therapeutic applications and biological mechanisms.

Neurosci Biobehav Rev 2021 08 21;127:37-53. Epub 2021 Apr 21.

Department of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. Electronic address:

Brain diseases, including neurodegenerative, cerebrovascular and neuropsychiatric diseases, have posed a deleterious threat to human health and brought a great burden to society and the healthcare system. With the development of medical technology, vagus nerve stimulation (VNS) has been approved by the Food and Drug Administration (FDA) as an alternative treatment for refractory epilepsy, refractory depression, cluster headaches, and migraines. Furthermore, current evidence showed promising results towards the treatment of more brain diseases, such as Parkinson's disease (PD), autistic spectrum disorder (ASD), traumatic brain injury (TBI), and stroke. Nonetheless, the biological mechanisms underlying the beneficial effects of VNS in brain diseases remain only partially elucidated. This review aims to delve into the relevant preclinical and clinical studies and update the progress of VNS applications and its potential mechanisms underlying the biological effects in brain diseases.
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http://dx.doi.org/10.1016/j.neubiorev.2021.04.018DOI Listing
August 2021

Nitric Oxide in the Spinal Cord Is Involved in the Hyperalgesia Induced by Tetrahydrobiopterin in Chronic Restraint Stress Rats.

Front Neurosci 2021 26;15:593654. Epub 2021 Mar 26.

Department of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

It has been well recognized that exposure to chronic stress could increase pain responding and exacerbate pain symptoms, resulting in stress-induced hyperalgesia. However, the mechanisms underlying stress-induced hyperalgesia are not yet fully elucidated. To this end, we observed that restraint as a stressful event exacerbated mechanical and thermal hyperalgesia, accompanied with up-regulation of nitric oxide (NO) ( < 0.001), GTP cyclohydrolase 1 (GCH1) (GCH1 mRNA: = 0.001; GCH1 protein: = 0.001), and tetrahydrobiopterin (BH4) concentration (plasma BH4: < 0.001; spinal BH4: < 0.001) on Day 7 in restraint stress (RS) rats. Intrathecal injection of -nitro-L-arginine methyl ester (L-NAME), a non-specific NO synthase inhibitor, or -([3-(aminomethyl)phenyl]methyl) ethanimidamide, a special inhibitor of inducible NO synthase (iNOS), for seven consecutive days attenuated stress-induced hyperalgesia and decreased the production of NO ( < 0.001). Interestingly, 7-nitro indazole, a special inhibitor of neuronal NO synthase, alleviated stress-induced hyperalgesia but did not affect spinal NO synthesis. Furthermore, intrathecal injection of BH4 not only aggravated stress-induced hyperalgesia but also up-regulated the expression of spinal iNOS (iNOS mRNA: = 0.015; iNOS protein: < 0.001) and NO production ( < 0.001). These findings suggest that hyperalgesia induced by RS is associated with the modulation of the GCH1-BH4 system and constitutively expressed spinal iNOS. Thus, the GCH1-BH4-iNOS signaling pathway may be a new novel therapeutic target for pain relief in the spinal cord.
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http://dx.doi.org/10.3389/fnins.2021.593654DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044835PMC
March 2021

Deficiency Attenuates Diet-Induced Obesity and Insulin Resistance by Promoting Fatty Acid Oxidation and Thermogenesis in Brown Adipocytes.

Adv Sci (Weinh) 2021 Mar 1;8(6):2002794. Epub 2021 Feb 1.

State Key Laboratory of Cancer Biology and Department of Physiology and Pathophysiology Fourth Military Medical University Xi'an Shaanxi 710032 China.

Altering the balance between energy intake and expenditure is a major strategy for treating obesity. Nonetheless, despite the progression in antiobesity drugs on appetite suppression, therapies aimed at increasing energy expenditure are limited. Here, knockout of, a signaling hub on outer mitochondrial membrane, renders mice resistant to diet-induced obesity. knockout significantly enhances energy expenditure and thermogenesis in brown adipose tissues (BATs) of obese mice. Restoring AKAP1 expression in BAT clearly reverses the beneficial antiobesity effect in mice. Mechanistically, AKAP1 remarkably decreases fatty acid β-oxidation (FAO) by phosphorylating ACSL1 to inhibit its activity in a protein-kinase-A-dependent manner and thus inhibits thermogenesis in brown adipocytes. Importantly, AKAP1 peptide inhibitor effectively alleviates diet-induced obesity and insulin resistance. Altogether, the findings demonstrate that AKAP1 functions as a brake of FAO to promote diet-induced obesity, which may be used as a potential therapeutic target for obesity.
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http://dx.doi.org/10.1002/advs.202002794DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7967052PMC
March 2021

COVID-19-related Traumatic Effects and Psychological Reactions among International Students.

J Epidemiol Glob Health 2021 03 24;11(1):117-123. Epub 2020 Oct 24.

Department of Anesthesiology, Shengjing Hospital of China Medical University, No 36, Sanhao Street, Heping District, Shenyang 110004, Liaoning Province, PR China.

Objective: The Coronavirus Disease 2019 (COVID-19) pandemic is a public health emergency of international concern and poses a challenge to people's psychological resilience. Students are reported to have greater psychological impacts from COVID-19. This study aimed to survey international students to better understand their traumatic effects and psychological reactions from COVID-19, to develop evidence-driven strategies to reduce adverse psychological impact during the pandemic.

Method: We conducted an online survey that collected information on the demographics, economic conditions, academic conditions, and health statuses of native Chinese students attending university in the U.S. Psychological impact was assessed by the Post-traumatic Stress Disorder (PTSD) Checklist Civilian Version (PCL-C) and mental health status was assessed by the Depression, Anxiety, and Stress Scale.

Results: This study included 261 Chinese international students. In total, 37.5% of respondents' PTSD PCL-C scores measured as moderate or severe. International students who were currently in China facing job-hunting or planning to continue studying abroad, severe economic pressure, and poor self-rated health status were significantly associated with greater PTSD PCL-C scores and higher levels of stress, anxiety, and depression.

Conclusion: During the COVID-19 pandemic, more than one-third of the respondents rated their PTSD PCL-C score as moderate-to-severe and nearly half of them reported moderate-to-severe anxiety. Our findings identify factors such as future academic plan, economic pressure, and health status are associated with higher levels of psychological impact and worse mental health status. These should receive attention and psychological interventions should be implemented to improve the mental health of international students during the COVID-19 pandemic.
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http://dx.doi.org/10.2991/jegh.k.201016.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7958271PMC
March 2021

Resveratrol Mitigates Hippocampal Tau Acetylation and Cognitive Deficit by Activation SIRT1 in Aged Rats following Anesthesia and Surgery.

Oxid Med Cell Longev 2020 16;2020:4635163. Epub 2020 Dec 16.

Department of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan, 430030 Hubei, China.

Postoperative cognitive dysfunction (POCD) is a sever postsurgical neurological complication in the elderly population. As the global acceleration of population ageing, POCD is proved to be a great challenge to the present labor market and healthcare system. In the present study, our findings showed that tau acetylation mediated by SIRT1 deficiency resulted in tau hyperphosphorylation in the hippocampus of the aged POCD model and consequently contributed to cognitive impairment. Interestingly, pretreatment with resveratrol almost restored the expression of SIRT1, reduced the levels of acetylated tau and hyperphosphorylated tau in the hippocampus, and improved the cognitive performance in the behavioral tests. What is more, we observed that microglia-derived neuroinflammation resulting from SIRT1 inhibition in microglia probably aggravated the tau acetylation in cultured neurons in vitro. Our findings supported the notion that activation SIRT1 provided dually beneficial effect in the aged POCD model. Taken together, our findings provided the initial evidence that tau acetylation was associated with cognitive impairment in the aged POCD model and paved a promising avenue to prevent POCD by inhibiting tau acetylation in a SIRT1-dependent manner.
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http://dx.doi.org/10.1155/2020/4635163DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7758127PMC
September 2021

Effect of dexmedetomidine on delirium during sedation in adult patients in intensive care units: A systematic review and meta-analysis.

J Clin Anesth 2021 May 3;69:110157. Epub 2020 Dec 3.

Department of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. Electronic address:

Study Objective: To compare the effect of sedation protocols with and without dexmedetomidine on delirium risk and duration in adult patients in intensive care units (ICUs).

Design: A meta-analysis of randomized controlled trials.

Review Methods: We searched the Cochrane Central Register of Controlled Trials, PubMed, EMBASE, and ISI Web of Science from inception to September 3, 2020. We included studies comparing the effect of dexmedetomidine-based sedation on delirium risk with non-dexmedetomidine-based sedation in adult patients in ICUs. We pooled the data using a random-effects model using Review Manager 5.2, and assessed publication bias using Stata 11.0. The quality of evidence was rated using the Grading of Recommendations, Assessment, Development and Evaluation system.

Main Results: We included 36 studies involving 9623 participants. The use of dexmedetomidine was associated with reduced risk of delirium (risk ratio [RR], 0.63; 95% confidence interval [CI], 0.54-0.75; very low-quality evidence), but higher incidences of hypotension and bradycardia during hospital stay. Dexmedetomidine was also associated with shorter durations of ICU stay, hospital stay and mechanical ventilation. Dexmedetomidine did not affect ICU mortality (RR, 1.01; 95% CI, 0.89-1.14; low-quality evidence), hospital mortality (RR, 1.01; 95% CI, 0.91-1.12; very low-quality evidence), or 30-day mortality (RR, 0.77; 95% CI, 0.58-1.01; moderate-quality evidence), or duration of delirium (mean difference, -0.74 days; 95% CI, -1.83 to 0.36 days; very low-quality evidence). We identified publication bias for risk and duration of delirium, length of ICU stay, and hospital stay.

Conclusions: Low- or very low-quality evidence suggests that dexmedetomidine was associated with a clinically-small reduction of delirium risk, ICU/hospital stay and mechanical ventilation duration, but were not associated with improved mortality or shorter delirium duration in ICU patients. These findings were inconclusive because of publication bias, heterogeneity, and limited sample size. Significant adverse effects of dexmedetomidine include hypotension and bradycardia. PROSPERO registration number: CRD42018095358.
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http://dx.doi.org/10.1016/j.jclinane.2020.110157DOI Listing
May 2021

Deep learning-based model for detecting 2019 novel coronavirus pneumonia on high-resolution computed tomography.

Sci Rep 2020 11 5;10(1):19196. Epub 2020 Nov 5.

Department of Internal Medicine, Renmin Hospital of Wuhan University, 99 Zhangzhidong Road, Wuhan, 430060, Hubei Province, China.

Computed tomography (CT) is the preferred imaging method for diagnosing 2019 novel coronavirus (COVID19) pneumonia. We aimed to construct a system based on deep learning for detecting COVID-19 pneumonia on high resolution CT. For model development and validation, 46,096 anonymous images from 106 admitted patients, including 51 patients of laboratory confirmed COVID-19 pneumonia and 55 control patients of other diseases in Renmin Hospital of Wuhan University were retrospectively collected. Twenty-seven prospective consecutive patients in Renmin Hospital of Wuhan University were collected to evaluate the efficiency of radiologists against 2019-CoV pneumonia with that of the model. An external test was conducted in Qianjiang Central Hospital to estimate the system's robustness. The model achieved a per-patient accuracy of 95.24% and a per-image accuracy of 98.85% in internal retrospective dataset. For 27 internal prospective patients, the system achieved a comparable performance to that of expert radiologist. In external dataset, it achieved an accuracy of 96%. With the assistance of the model, the reading time of radiologists was greatly decreased by 65%. The deep learning model showed a comparable performance with expert radiologist, and greatly improved the efficiency of radiologists in clinical practice.
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http://dx.doi.org/10.1038/s41598-020-76282-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7645624PMC
November 2020

Correlation between Mitochondrial Dysfunction, Cardiovascular Diseases, and Traditional Chinese Medicine.

Evid Based Complement Alternat Med 2020 7;2020:2902136. Epub 2020 Oct 7.

The First Affiliated Hospital of Xinxiang Medical University, Xinxiang 453100, Henan, China.

Cardiovascular disease (CVD) is the number one threat that seriously endangers human health. However, the mechanism of their occurrence is not completely clear. Increasing studies showed that mitochondrial dysfunction is closely related to CVD. Possible causes of mitochondrial dysfunction include oxidative stress, Ca disorder, mitochondrial DNA mutations, and reduction of mitochondrial biosynthesis, all of which are closely related to the development of CVD. At present, traditional Chinese medicine (TCM) is widely used in the treatment of CVD. TCM has the therapeutic characteristics of multitargets and multipathways. Studies have shown that TCM can treat CVD by protecting mitochondrial function. Via systematic literature review, the results show that the specific mechanisms include antioxidant stress, regulation of calcium homeostasis, antiapoptosis, and regulation of mitochondrial biosynthesis. This article describes the relationship between mitochondrial dysfunction and CVD, summarizes the TCM commonly used for the treatment of CVD in recent years, and focuses on the regulatory effect of TCM on mitochondrial function.
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http://dx.doi.org/10.1155/2020/2902136DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7568168PMC
October 2020

Synthesis of difluoroalkylated 2-azaspiro[4.5]decane derivatives via copper-catalyzed difluoroalkylation/dearomatization of N-benzylacrylamides.

Org Biomol Chem 2020 Nov 19;18(41):8376-8380. Epub 2020 Oct 19.

Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, Tianjin 300070, P. R. China.

An efficient method for the synthesis of difluoroalkylated 2-azaspiro[4.5]decanes via copper-catalyzed difluoroalkylation of N-benzylacrylamides with ethyl bromodifluoroacetate has been established. The reaction experienced a tandem radical addition and dearomatizing cyclization process. In addition, the resultant products can be smoothly converted into a difluoroalkylated quinolinone and saturated spirocyclohexanone scaffold.
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http://dx.doi.org/10.1039/d0ob01833cDOI Listing
November 2020

Salidroside downregulates microRNA‑133a and inhibits endothelial cell apoptosis induced by oxidized low‑density lipoprotein.

Int J Mol Med 2020 Oct 31;46(4):1433-1442. Epub 2020 Jul 31.

The First Affiliated Hospital of Xinxiang Medical University, Henan Engineering Research Center for Mitochondrion Biomedical of Heart, Heart Center, Xinxiang, Henan 453100, P.R. China.

Vascular endothelial cell apoptosis is regulated by microRNA‑133a (miR‑133a), which participates in the formation of atherosclerotic (AS) plaques, leading to the development of several cardiovascular diseases. Salidroside (SAL), the main component of Rhodiola, is considered to exert anti‑AS effect; however, its mode of action remains unclear. Thus, the present study aimed to determine whether SAL inhibits endothelial cell apoptosis through the miR‑133a pathway. Cultured human coronary artery endothelial cells (HCAECs) were exposed to oxidized low‑density lipoprotein (ox‑LDL). Cell viability and cytotoxicity were monitored by MTT assay. In parallel, the mRNA expression levels of miR‑133a and Bcl‑xL, and the protein levels of anti‑apoptotic Bcl‑xL and activated caspase‑3 were measured. The apoptotic levels were examined by flow cytometry. Furthermore, the effects of silencing and overexpressing miR‑133a on the parameters mentioned above were evaluated. Exposure to ox‑LDL induced an increase in the expression of miR‑133a, with a concomitant decrease in the level of Bcl‑xL in the HCAECs; these effects were reversed by treatment with SAL. Importantly, the effects of SAL were impaired upon the silencing of miR‑133a, whereas the overexpression of miR‑133a partly restored the effects of SAL. On the whole, the findings of the present study demonstrate that SAL inhibits the ox‑LDL‑induced upregulation of miR‑133a expression, while promoting the expression of Bcl‑xL, thereby preventing endothelial cell apoptosis.
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http://dx.doi.org/10.3892/ijmm.2020.4691DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7447316PMC
October 2020

CaMKII oxidation regulates cockroach allergen-induced mitophagy in asthma.

J Allergy Clin Immunol 2021 04 10;147(4):1464-1477.e11. Epub 2020 Sep 10.

Johns Hopkins Asthma & Allergy Center, Johns Hopkins University School of Medicine, Baltimore, Md. Electronic address:

Background: Autophagy plays an important role in causing inflammatory responses initiated by environmental pollutants and respiratory tract infection.

Objective: We sought to investigate the role of cockroach allergen-induced excessive activation of autophagy in allergic airway inflammation and its underlying molecular mechanisms.

Methods: Environmental allergen-induced autophagy was investigated in the primary human bronchial epithelial cells (HBECs) and lung tissues of asthmatic mouse model and patients. The role of autophagy in asthma development was examined by using autophagy inhibitor 3-methyladenine in an asthma mouse model. Furthermore, the involvements of reactive oxygen species (ROS) and oxidized Ca/calmodulin-dependent protein kinase II (ox-CaMKII) signaling in regulating autophagy during asthma were examined in allergen-treated HBECs and mouse model.

Results: Cockroach allergen activated autophagy in HBECs and in the lung tissues from asthmatic patients and mice. Autophagy inhibitor 3-methyladenine significantly attenuated airway hyperresponsiveness, T2-associated lung inflammation, and ROS generation. Mechanistically, we demonstrated a pathological feedforward circuit between cockroach allergen-induced ROS and autophagy that is mediated through CaMKII oxidation. Furthermore, transgenic mice with ROS-resistant CaMKII MM-VVδ showed attenuation of T2-associated lung inflammation and autophagy. Mitochondrial ox-CaMKII inhibition induced by adenovirus carrying mitochondrial-targeted inhibitor peptide CaMKIIN suppresses cockroach allergen-induced autophagy, mitochondrial dysfunction, mitophagy, and cytokine production in HBECs. Finally, mitochondrial CaMKII inhibition suppressed the expression of one of the key ubiquitin-binding autophagy receptors, optineurin, and its recruitment to fragmented mitochondria. Optineurin knockdown inhibited cockroach allergy-induced mitophagy.

Conclusions: Our data suggest a previously uncovered axis of allergen-ROS-ox-CaMKII-mitophagy in the development of allergic airway inflammation and asthma.
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http://dx.doi.org/10.1016/j.jaci.2020.08.033DOI Listing
April 2021

Six-Gene Signature Associated with Immune Cells in the Progression of Atherosclerosis Discovered by Comprehensive Bioinformatics Analyses.

Cardiovasc Ther 2020 25;2020:1230513. Epub 2020 Jul 25.

Department of Oncology and Vascular Interventional Radiology, Zhongshan Hospital Affiliated to Xiamen University, Xiamen, Fujian, China.

Background: As a multifaceted disease, atherosclerosis is often characterized by the formation and accumulation of plaque anchored to the inner wall of the arteries and causes some cardiovascular diseases and vascular embolism. Numerous studies have reported on the pathogenesis of atherosclerosis. However, fewer studies focused on both genes and immune cells, and the correlation of genes and immune cells was evaluated via comprehensive bioinformatics analyses.

Methods: 29 samples of atherosclerosis-related gene expression profiling, including 16 human advanced atherosclerosis plaque (AA) and 13 human early atherosclerosis plaque (EA) samples from the Gene Expression Omnibus (GEO) database, were analyzed to get differentially expressed genes (DEGs) and the construction of protein and protein interaction (PPI) networks. Besides, we detected the relative fraction of 22 immune cell types in atherosclerosis by using the deconvolution algorithm of "cell type identification by estimating relative subsets of RNA transcripts (CIBERSORT)." Ultimately, based on the significantly changed types of immune cells, we executed the correlation analysis between DEGs and immune cells to discover the potential genes and pathways associated with immune cells.

Results: We identified 17 module genes and 6 types of significantly changed immune cells. Correlation analysis showed that the relative percentage of T cell CD8 has negative correlation with the expression ( = -0.63, = 0.02), and the relative percentage of macrophage M2 has positive correlation with the expression ( = 0.57, = 0.041) in EA. Meanwhile, four gene expressions (, , , and ) have a high correlation with the percentages of T cell CD8 and macrophages (M0 and M2) in AA samples.

Conclusions: In this study, we suggested that the progression of atherosclerosis might be related to , , , , , and and that it plays a role in regulating immune-competent cells such as T cell CD8 and macrophages M0 and M2. These results will enable studies of the potential genes associated with immune cells in the progression of atherosclerosis, as well as provide insight for discovering new treatments and drugs.
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http://dx.doi.org/10.1155/2020/1230513DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7416237PMC
September 2020
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