Publications by authors named "Yilin Li"

113 Publications

Comparison of the complications between minimally invasive surgery and open surgical treatments for early-stage cervical cancer: A systematic review and meta-analysis.

PLoS One 2021 1;16(7):e0253143. Epub 2021 Jul 1.

Division of Gynecology and Obstetrics, Life Science Park of Zhongguancun, Peking University International Hospital, Changping District, Beijing, China.

Background: This meta-analysis comprehensively compared intraoperative and postoperative complications between minimally invasive surgery (MIS) and laparotomy in the management of cervical cancer. Even though the advantages of laparotomy over MIS in disease-free survival and overall survival for management of gynecological diseases have been cited in the literature, there is a lack of substantial evidence of the advantage of one surgical modality over another, and it is uncertain whether MIS is justifiable in terms of safety and efficacy.

Methods: In this meta-analysis, the studies were abstracted that the outcomes of complications to compare MIS (laparoscopic or robot-assisted) and open radical hysterectomy in patients with early-stage (International Federation of Gynecology and Obstetrics classification stage IA1-IIB) cervical cancer. The primary outcomes were intraoperative overall complications, as well as postoperative aggregate complications. Secondary outcomes included the individual complications. Two investigators independently performed the screening and data extraction. All articles that met the eligibility criteria were included in this meta-analysis.

Results: The meta-analysis finally included 39 non-randomized studies and 1 randomized controlled trial (8 studies were conducted on robotic radical hysterectomy (RRH) vs open radical hysterectomy (ORH), 27 studies were conducted on laparoscopic radical hysterectomy (LRH) vs ORH, and 5 studies were conducted on all three approaches). Pooled analyses showed that MIS was associated with higher risk of intraoperative overall complications (OR = 1.41, 95% CI = 1.07-1.86, P<0.05) in comparison with ORH. However, compared to ORH, MIS was associated with significantly lower risk of postoperative aggregate complications (OR = 0.40, 95% CI = 0.34-0.48, P = 0.0143). In terms of individual complications, MIS appeared to have a positive effect in decreasing the complications of transfusion, wound infection, pelvic infection and abscess, lymphedema, intestinal obstruction, pulmonary embolism, deep vein thrombosis, and urinary tract infection. Furthermore, MIS had a negative effect in increasing the complications of cystotomy, bowel injury, subcutaneous emphysema, and fistula.

Conclusions: Our meta-analysis demonstrates that MIS is superior to laparotomy, with fewer postoperative overall complications (wound infection, pelvic infection and abscess, lymphedema, intestinal obstruction, pulmonary embolism, and urinary tract infection). However, MIS is associated with a higher risk of intraoperative aggregate complications (cystotomy, bowel injury, and subcutaneous emphysema) and postoperative fistula complications.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0253143PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8248723PMC
July 2021

Oxypeucedanin is a mechanism-based inactivator of CYP2B6 and CYP2D6.

Curr Drug Metab 2021 Jun 29. Epub 2021 Jun 29.

Wuya College of Innovation, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, Liaoning, 110016, China.

Background: Herbal medicine Angelica dahurica is widely employed for the treatment of rheumatism and pain relief in China. Oxypeucedanin is a major component of the herb.

Objectives: The objectives of this study are aimed at the investigation of mechanism-based inactivation of CYP2B6 and CYP2D6 by oxypeucedanin, characterization of the reactive metabolites associated with the enzyme inactivation, and identification of the P450s participating in the bioactivation of oxypeucedanin.

Methods: Oxypeucedanin was incubated with liver microsomes or recombinant CYPs2B6 and 2D6 under designed conditions, and the enzyme activities were measured by monitoring the generation of the corresponding products. The resulting reactive intermediates were trapped with GSH and analyzed by LC-MS/MS.

Results: Microsomal incubation with oxypeucedanin induced a time-, concentration-, and NADPH-dependent inhibition of CYPs2B6 and 2D6 with kinetic values of KI/kinact 1.82 µM/0.07 min-1 (CYP2B6) and 8.47 µM/0.044 min-1 (CYP2D6), respectively. Ticlopidine and quinidine attenuated the observed time-dependent enzyme inhibitions. An epoxide and/or γ-ketoenal intermediate(s) derived from oxypeucedanin was/were trapped in microsomal incubations. CYP3A4 was the primary enzyme involved in the bioactivation of oxypeucedanin.

Conclusion: Oxypeucedanin was a mechanism-based inactivator of CYP2B6 and CYP2D6. An epoxide and/or γ-ketoenal intermediate(s) may be responsible for the inactivation of the two enzymes.
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http://dx.doi.org/10.2174/1389200222666210629114830DOI Listing
June 2021

Efficacy and safety of hyperbaric oxygen therapy for moderate-to-severe ulcerative colitis: a protocol for a systematic review and meta-analysis.

BMJ Open 2021 06 28;11(6):e047543. Epub 2021 Jun 28.

Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan Province, China

Introduction: Ulcerative colitis (UC) is a type of inflammatory bowel disease, and 62% of patients with UC felt that it is difficult for them to live a normal life. Furthermore, some researches have shown that about 15% of patients with UC undergo at least one extreme clinical course in their lifetime, and 10%-30% of patients with UC oblige colectomy. Although many investigations have demonstrated that HBO has a beneficial impact on UC treatment, a systematic review and meta-analysis are unavailable. Therefore, a meta-analysis is essential to assess the efficacy and safety of HBO in treating UC.

Methods And Analysis: A systematic search plan will be performed in the following seven databases with a restriction of time from inception to September 2020 to filter the eligible studies: PubMed, Web of Science, Embase, Cochrane Library, China National Knowledge Infrastructure, Chinese Scientific Journal Database (VIP) and Chinese Biomedical Database WanFang. Other related resources will be also searched. Two independent reviewers will choose eligible researches and extract data. The risk of bias will be evaluated based on Cochrane Collaboration's Risk of Bias tool and Newcastle-Ottawa Scale. Eventually, a systematic review and meta-analysis will be performed via the Review Manager V.5.3 statistical software and STATA V.14.0 software.

Ethics And Dissemination: This study will not involve the individual patient and any ethical problems since its outcomes are based on published data. Therefore, no ethical review and approval are required. We plan to publish the study in a peer-reviewed journal.

Prospero Registration Number: CRD42020210244.
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http://dx.doi.org/10.1136/bmjopen-2020-047543DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8240565PMC
June 2021

Glutathione conjugation and protein modification resulting from metabolic activation of venlafaxine and .

Xenobiotica 2021 Jun 23:1-39. Epub 2021 Jun 23.

Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, P. R. China.

Venlafaxine (VLF), an antidepressant agent, is widely used to combat major depressive disorders, particularly for the treatment of selective serotonin reuptake inhibitor-resistant depression. VLF has been shown to cause liver injury. The present study aimed to investigate the metabolic activation of VLF and explore the mechanisms of hepatotoxicity induced by VLF.One glutathione (GSH) conjugate and one cysteine conjugate were both detected in mouse and human liver microsomal incubations containing VLF and GSH or cysteine. The two conjugates were also detected in cultured mouse primary hepatocytes and bile of rats after exposure to VLF. The and studies demonstrated that VLF was metabolized to a quinone methide intermediate reactive to GSH and cysteine residues of hepatic protein. The observed protein covalent binding revealed dose-dependency. The metabolic activation of VLF was P450-dependent, and CYP3A4 was found as the predominant enzyme involved in the bioactivation process.These findings facilitate better understanding of the metabolic activation-hepatotoxicity relationship of VLF and provide chemists with information about new potential structural alerts during drug design process.
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http://dx.doi.org/10.1080/00498254.2021.1946204DOI Listing
June 2021

Profiling heterogenous sizes of circulating tumor microemboli to track therapeutic resistance and prognosis in advanced gastric cancer.

Hum Cell 2021 Jun 21. Epub 2021 Jun 21.

Department of Gastrointestinal Oncology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital and Institute, Fucheng Road 52, Haidian District, Beijing, 100142, China.

Circulating tumor microemboli (CTM) aggregated by ≥ 2 circulating tumor cells (CTCs) are more migratory than single CTCs. Aside from the plasticity in their molecular characteristics, which have been considered tumor migration, CTM also possesses high size heterogeneity. This study, therefore, systematically investigated the heterogeneous sizes of CTM and their involvement in therapeutic resistance in 114 patients with advanced gastric cancer (GC) using a pre-established surface molecule-independent subtraction enrichment (SE)-iFISH strategy. CTM, which was pre-therapeutically detected in 33.3% of GC patients, can further form in another 34.78% of patients following chemo-/targeted therapies. The presence of CTM is relevant to liver metastasis as well as higher CTC levels (≥ 5/6 mL). Further size-based profiling of GC-CTM revealed that CTM with 2 CTCs (CTM) was the dominant subtype, accounting for 50.0% of all detected GC-CTMs. However, CTM with 3-4 CTCs (CTM) specifically associates with chemo-/targeted therapeutic resistance and inferior prognosis. Patients with ≥ 1 CTM/6 mL have shorter median progression-free survival and median overall survival. Unlike CTM and CTM, which are detectable in pre-therapy and post-therapy, larger aggregated CTM (CTM with ≥ 5 CTCs) was only intra-therapeutically detected in four HER2 GC patients, of which three experienced liver metastases. Obtained results suggested that the cluster size of GC-CTM should be dynamically profiled beyond pre-therapeutic whole CTM enumeration in terms of chemo-/targeted resistance or metastasis monitoring. GC-CTM could be a potential indicator of therapeutic resistance, while the dynamic presence of GC-CTM implies liver metastasis in HER2 GC patients.
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http://dx.doi.org/10.1007/s13577-021-00568-2DOI Listing
June 2021

The effect of quercetin on cervical cancer cells as determined by inducing tumor endoplasmic reticulum stress and apoptosis and its mechanism of action.

Am J Transl Res 2021 15;13(5):5240-5247. Epub 2021 May 15.

Department of Gynecology, Gansu Provincial Maternity and Child-care Hospital Lanzhou 730050, Gansu Province, P. R. China.

Objective: This study aimed to explore the effect of quercetin on cervical cancer cells by inducing tumor endoplasmic reticulum stress (ERS) and apoptosis and its mechanism of action.

Methods: HeLa cells were treated with different concentrations of quercetin, and the cell viability was measured using methyl thiazolyl tetrazolium (MTT) colorimetric assays. The apoptosis rate was measured using flow cytometry. The changes in the related protein X (Bax), B-cell lymphoma/leukemia-2 (Bcl-2), and G1/S-specific cyclin-D1 (Cyclin D1) levels after the HeLe apoptosis were determined using Western blot, and the changes in the human cystinase-3 (Caspase-3), glucoprotein 78 (GRP78), and enhancer-binding protein homologous protein (CHOP) levels, and the receptor-related protein levels in the ERS pathway/endoribonuclease inositol requiring enzyme 1 (IRE1), and the phosphorylated pancreatic endoplasmic reticulum stress kinase (p-Perk), and the activated transcription factor-6 (ATF6) levels were also quantified.

Results: After treating the HeLa cells with different concentrations of quercetin, the cell viability was inhibited to varying degrees, showing a significant time and concentration dependence. The apoptosis rate in the quercetin group increased significantly in comparison with the blank control group, and the apoptosis rate also showed a tendency to increase progressively with an increasing concentration of the quercetin (P<0.05). The Bax and Bcl-2 levels in the quercetin intervention group showed a tendency to increase progressively in comparison with the blank control group, and Cyclin D1 showed a tendency to decrease progressively (P<0.05). The of Caspase-3, GRP78, and CHOP expression levels in the quercetin intervention group rose significantly in comparison with the blank control group (P<0.05). The IRE1, p-Perk, and c-ATF6 levels in the quercetin intervention group showed a tendency to rise gradually in comparison with the blank control group (P<0.05).

Conclusion: Quercetin may promote the apoptosis of cervical cancer HeLe cells by inducing the tumor ERS pathway.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205781PMC
May 2021

Dysregulated KRAS gene-signaling axis and abnormal chromatin remodeling drive therapeutic resistance in heterogeneous-sized circulating tumor cells in gastric cancer patients.

Cancer Lett 2021 Oct 11;517:78-87. Epub 2021 Jun 11.

Department of Gastrointestinal Oncology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital and Institute, Beijing, China. Electronic address:

The mechanism by which heterogeneous-sized circulating tumor cells (CTCs) in gastric cancer (GC) patients are resistant to the targeted therapy and/or chemotherapy remains unclear. This study investigated prognostic value and genomic variations of size-heterogenous CTCs, in an attempt to unravel the molecular mechanisms underlying the therapeutic resistance, which is relevant to poor prognosis in GC. Aneuploid CTCs, detected in 111 advanced GC patients, were categorized into small (≤white blood cell [WBC], 25.54%) and large (>WBC, 74.46%) cells. Pre-treatment patients possessing ≥3 baseline small CTCs with trisomy 8 (CTCs) or ≥6 large multiploid CTCs (CTCs) showed an inferior median progression-free survival. Moreover, the cut-off value of ≥6 CTCs was also an effective prognosticator for poor median overall survival. Single cell-based DNA sequencing of 50 targeted CTCs indicated that CTCs and CTCs harbored distinct gene variations respectively. Mutations in the KRAS and Rap1 pathway were remarkably abundant in CTCs, whereas several unique mutations in the MET/PI3K/AKT pathway and SMARCB1 gene were identified in CTCs. Obtained results suggested that CTCs and CTCs exhibited different mechanisms to therapy resistance and correlated with patients' poor outcome.
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http://dx.doi.org/10.1016/j.canlet.2021.06.002DOI Listing
October 2021

Role of ultramicropores in the remarkable gas storage in hypercrosslinked polystyrene networks studied by positron annihilation.

Phys Chem Chem Phys 2021 Jun;23(24):13603-13611

Hubei Nuclear Solid Physics Key Laboratory, Department of Physics, Wuhan University, Wuhan 430072, China.

In this paper, hypercrosslinked polystyrene (HCLPS) networks were synthesized by radical bulk polymerization and Friedel-Crafts alkylation reactions using vinylbenzyl-co-divinylbenzene chloride (VBC-DVB) as the precursors. A series of HCLPS was prepared with varying content of DVB from 0 to 10% in the precursor. Both N2 adsorption and positron annihilation measurements reveal micropores in the HCLPS. Especially, the existence of ultramicropores with a size in the range of 0.63-0.7 nm is confirmed by positron lifetime measurements. With increasing DVB content from 0 to 10%, the number of ultramicropores shows a gradual increase. Both the H2 and CO2 adsorption capacity increase monotonously with the increase of the DVB content. With 10% DVB in the HCLPS, the H2 storage increases to 10.3 mmol g-1 (2.05 wt%) at 77 K and 1 bar and the CO2 capture reaches 2.81 mmol g-1 (12.4 wt%) at 273 K and 1 bar. The remarkable gas storage ability is ascribed to the existence of the ultramicropores, which result in a stronger affinity to the gas molecules. By using positrons as a new probe for the pores, our results provide convincing evidence of the role of ultramicropores in the gas adsorption performance in microporous organic polymers.
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http://dx.doi.org/10.1039/d1cp01867aDOI Listing
June 2021

Determining optimal gestational weight gain (GWG) in a northwest Chinese population: A CONSORT.

Medicine (Baltimore) 2021 May;100(21):e26080

Gansu Provincial Maternity and Child Care Hospital, Qilihe District, Lanzhou, Gansu, China.

Abstract: To determine optimal gestational weight gain (GWG) for the Chinese population.Live singleton deliveries at the largest maternal & childcare hospital in northwest China from 2010 to 2012 were analyzed retrospectively. Multivariable logistic regression analysis was conducted to determine the lowest aggregated risk of interested perinatal outcomes based on Chinese adult body mass index (BMI) categories.Eight thousand eight hundred seventy enrolled parturients were divided into 4 groups according to their prepregnancy BMI: underweight (21.31%, BMI < 18.5 kg/m2), normal weight (67.81%, 18.5 kg/m2 ≤ BMI < 24 kg/m2), overweight (8.99%, 24 kg/m2 ≤ BMI < 28 kg/m2 and obese (1.89%, BMI ≥ 28 kg/m2). The optimal GWG values for the above 4 groups were 16.7 kg (GWG range, 12.0-21.5), 14.5 kg (9.5-19.5), 11.5 kg (7.0-16.5), and 8.0 kg (5.0-13.0). The rates of inadequate, optimal and excessive GWG in present study were 6.14% (545), 62.34% (5529), and 31.52% (2796) respectively, which were significantly different from those of the 2009 Institute of Medicine recommendation (χ2 = 1416.05, Pinteraction < 0.0001).Wider optimal GWG ranges than those recommended by Institute of Medicine were found in our study, and our proposed criteria seems to be practical to the Chinese population.
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http://dx.doi.org/10.1097/MD.0000000000026080DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154430PMC
May 2021

Discovery of Novel Benzothiazepinones as Irreversible Covalent Glycogen Synthase Kinase 3β Inhibitors for the Treatment of Acute Promyelocytic Leukemia.

J Med Chem 2021 06 24;64(11):7341-7358. Epub 2021 May 24.

Department of Medicinal Chemistry, School of Pharmacy, Fudan University, Shanghai 201203, China.

Recently, irreversible inhibitors have attracted great interest in antitumors due to their advantages of forming covalent bonds to target proteins. Herein, some benzothiazepinone compounds (BTZs) have been designed and synthesized as novel covalent GSK-3β inhibitors with high selectivity for the kinase panel. The irreversible covalent binding mode was identified by kinetics and mass spectrometry, and the main labeled residue was confirmed to be the unique Cys14 that exists only in GSK-3β. The candidate - (IC = 6.6 μM) showed good proliferation inhibition and apoptosis-inducing ability to leukemia cell lines, low cytotoxicity on normal cell lines, and no hERG inhibition, which hinted the potential efficacy and safety. Furthermore, - exhibited decent pharmacokinetic properties and remarkably inhibited tumor growth in the acute promyelocytic leukemia (APL) mouse model. All the results suggest that these newly irreversible BTZ compounds might be useful in the treatment of cancer such as APL.
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http://dx.doi.org/10.1021/acs.jmedchem.0c02254DOI Listing
June 2021

The loss of RNA N-adenosine methyltransferase Mettl14 in tumor-associated macrophages promotes CD8 T cell dysfunction and tumor growth.

Cancer Cell 2021 Jul 20;39(7):945-957.e10. Epub 2021 May 20.

Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics, and China National Center for Bioinformation, Chinese Academy of Sciences, Beijing 100101, China; College of Future Technology, Sino-Danish College, University of Chinese Academy of Sciences, Beijing 100049, China; Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China; China National Center for Bioinformation, Beijing, 100101, China. Electronic address:

Tumor-associated macrophages (TAMs) can dampen the antitumor activity of T cells, yet the underlying mechanism remains incompletely understood. Here, we show that C1q TAMs are regulated by an RNA N-methyladenosine (mA) program and modulate tumor-infiltrating CD8 T cells by expressing multiple immunomodulatory ligands. Macrophage-specific knockout of an mA methyltransferase Mettl14 drives CD8 T cell differentiation along a dysfunctional trajectory, impairing CD8 T cells to eliminate tumors. Mettl14-deficient C1q TAMs show a decreased mA abundance on and a higher level of transcripts of Ebi3, a cytokine subunit. In addition, neutralization of EBI3 leads to reinvigoration of dysfunctional CD8 T cells and overcomes immunosuppressive impact in mice. We show that the METTL14-mA levels are negatively correlated with dysfunctional T cell levels in patients with colorectal cancer, supporting the clinical relevance of this regulatory pathway. Thus, our study demonstrates how an mA methyltransferase in TAMs promotes CD8 T cell dysfunction and tumor progression.
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http://dx.doi.org/10.1016/j.ccell.2021.04.016DOI Listing
July 2021

Tumors exploit FTO-mediated regulation of glycolytic metabolism to evade immune surveillance.

Cell Metab 2021 Jun 27;33(6):1221-1233.e11. Epub 2021 Apr 27.

Department of Basic Medical Sciences, School of Medicine, Institute for Immunology, Beijing Key Lab for Immunological Research on Chronic Diseases, THU-PKU Center for Life Sciences, Tsinghua University, Beijing 100084, China. Electronic address:

The ever-increasing understanding of the complexity of factors and regulatory layers that contribute to immune evasion facilitates the development of immunotherapies. However, the diversity of malignant tumors limits many known mechanisms in specific genetic and epigenetic contexts, manifesting the need to discover general driver genes. Here, we have identified the mA demethylase FTO as an essential epitranscriptomic regulator utilized by tumors to escape immune surveillance through regulation of glycolytic metabolism. We show that FTO-mediated mA demethylation in tumor cells elevates the transcription factors c-Jun, JunB, and C/EBPβ, which allows the rewiring of glycolytic metabolism. Fto knockdown impairs the glycolytic activity of tumor cells, which restores the function of CD8 T cells, thereby inhibiting tumor growth. Furthermore, we developed a small-molecule compound, Dac51, that can inhibit the activity of FTO, block FTO-mediated immune evasion, and synergize with checkpoint blockade for better tumor control, suggesting reprogramming RNA epitranscriptome as a potential strategy for immunotherapy.
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http://dx.doi.org/10.1016/j.cmet.2021.04.001DOI Listing
June 2021

Richer fusion network for breast cancer classification based on multimodal data.

BMC Med Inform Decis Mak 2021 04 22;21(Suppl 1):134. Epub 2021 Apr 22.

Department of Oncology, Peking University International Hospital, Beijing, China.

Background: Deep learning algorithms significantly improve the accuracy of pathological image classification, but the accuracy of breast cancer classification using only single-mode pathological images still cannot meet the needs of clinical practice. Inspired by the real scenario of pathologists reading pathological images for diagnosis, we integrate pathological images and structured data extracted from clinical electronic medical record (EMR) to further improve the accuracy of breast cancer classification.

Methods: In this paper, we propose a new richer fusion network for the classification of benign and malignant breast cancer based on multimodal data. To make pathological image can be integrated more sufficient with structured EMR data, we proposed a method to extract richer multilevel feature representation of the pathological image from multiple convolutional layers. Meanwhile, to minimize the information loss for each modality before data fusion, we use the denoising autoencoder as a way to increase the low-dimensional structured EMR data to high-dimensional, instead of reducing the high-dimensional image data to low-dimensional before data fusion. In addition, denoising autoencoder naturally generalizes our method to make the accurate prediction with partially missing structured EMR data.

Results: The experimental results show that the proposed method is superior to the most advanced method in terms of the average classification accuracy (92.9%). In addition, we have released a dataset containing structured data from 185 patients that were extracted from EMR and 3764 paired pathological images of breast cancer, which can be publicly downloaded from http://ear.ict.ac.cn/?page_id=1663 .

Conclusions: We utilized a new richer fusion network to integrate highly heterogeneous data to leverage the structured EMR data to improve the accuracy of pathological image classification. Therefore, the application of automatic breast cancer classification algorithms in clinical practice becomes possible. Due to the generality of the proposed fusion method, it can be straightforwardly extended to the fusion of other structured data and unstructured data.
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http://dx.doi.org/10.1186/s12911-020-01340-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8061018PMC
April 2021

Alloy-Electrode-Assisted High-Performance Enhancement-Type Neodymium-Doped Indium-Zinc-Oxide Thin-Film Transistors on Polyimide Flexible Substrate.

Research (Wash D C) 2021 22;2021:5758435. Epub 2021 Mar 22.

State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou 510640, China.

Flexible thin-film transistors with high current-driven capability are of great significance for the next-generation new display technology. The effect of a Cu-Cr-Zr (CCZ) copper alloy source/drain (S/D) electrode on flexible amorphous neodymium-doped indium-zinc-oxide thin-film transistors (NdIZO-TFTs) was investigated. Compared with pure copper (Cu) and aluminum (Al) S/D electrodes, the CCZ S/D electrode changes the TFT working mode from depletion mode to enhancement mode, which is ascribed to the alloy-assisted interface layer besides work function matching. X-ray photoelectron spectroscopy (XPS) depth profile analysis was conducted to examine the chemical states of the contact interface, and the result suggested that chromium (Cr) oxide and zirconium (Zr) oxide aggregate at the interface between the S/D electrode and the active layer, acting as a potential barrier against residual free electron carriers. The optimal NdIZO-TFT exhibited a desired performance with a saturation mobility ( ) of 40.3 cm·V·s, an / ratio of 1.24 × 10, a subthreshold swing (SS) value of 0.12 V·decade, and a threshold voltage ( ) of 0.83 V. This work is anticipated to provide a novel approach to the realization of high-performance flexible NdIZO-TFTs working in enhancement mode.
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http://dx.doi.org/10.34133/2021/5758435DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010622PMC
March 2021

Machine Learning-Based Radiomics Nomogram for Detecting Extramural Venous Invasion in Rectal Cancer.

Front Oncol 2021 26;11:610338. Epub 2021 Mar 26.

Department of Diagnostic Radiology, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, China.

Objective: To establish and validate a radiomics nomogram based on the features of the primary tumor for predicting preoperative pathological extramural venous invasion (EMVI) in rectal cancer using machine learning.

Methods: The clinical and imaging data of 281 patients with primary rectal cancer from April 2012 to May 2018 were retrospectively analyzed. All the patients were divided into a training set (n = 198) and a test set (n = 83) respectively. The radiomics features of the primary tumor were extracted from the enhanced computed tomography (CT), the T2-weighted imaging (T2WI) and the gadolinium contrast-enhanced T1-weighted imaging (CE-TIWI) of each patient. One optimal radiomics signature extracted from each modal image was generated by receiver operating characteristic (ROC) curve analysis after dimensionality reduction. Three kinds of models were constructed based on training set, including the clinical model (the optimal radiomics signature combining with the clinical features), the magnetic resonance imaging model (the optimal radiomics signature combining with the mrEMVI status) and the integrated model (the optimal radiomics signature combining with both the clinical features and the mrEMVI status). Finally, the optimal model was selected to create a radiomics nomogram. The performance of the nomogram to evaluate clinical efficacy was verified by ROC curves and decision curve analysis curves.

Results: The radiomics signature constructed based on T2WI showed the best performance, with an AUC value of 0.717, a sensitivity of 0.742 and a specificity of 0.621. The radiomics nomogram had the highest prediction efficiency, of which the AUC was 0.863, the sensitivity was 0.774 and the specificity was 0.801.

Conclusion: The radiomics nomogram had the highest efficiency in predicting EMVI. This may help patients choose the best treatment strategy and may strengthen personalized treatment methods to further optimize the treatment effect.
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http://dx.doi.org/10.3389/fonc.2021.610338DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8033032PMC
March 2021

The Effect of Neoadjuvant Chemotherapy Combined With Brachytherapy Before Radical Hysterectomy on Stage IB2 and IIA Cervical Cancer: A Retrospective Analysis.

Front Oncol 2021 23;11:618612. Epub 2021 Mar 23.

Gansu Provincial Maternity and Child Care Hospital, Lanzhou, China.

Objective: This study aims to retrospectively evaluate and compare the clinical efficacy in patients with stage IB2 and IIA cervical cancer, who treated with neoadjuvant chemotherapy combined with brachytherapy or not before radical hysterectomy.

Methods: The data of patients who have diagnosed with stage IB2 and IIA cervical cancer between January 2010 and December 2013 were retrieved through the Hospital Information System (HIS) of Gansu Provincial Maternal and Child Health Hospital. Patients were divided into two groups: neoadjuvant chemotherapy combined with brachytherapy followed by radical hysterectomy group (NACT+BT Group) and direct radical hysterectomy group (RH Group). The rate of adjuvant radiotherapy, progression-free survival (PFS), and overall survival (OS) were compared between the two groups.

Results: A total of 183 patients were included in this study with 82 in the NACT+BT group and 101 in the RH group. The median follow up duration was 44.9 months for the NACT+BT group and 38.1 months for the RH group. The 5-year PFS for NACT+BT Group was 93.8%, which was significantly higher compared to the RH group (77.2%, = 0.0202). The rate of postoperative adjuvant pelvic radiotherapy was significantly lower in the NACT+BT group compared to the RH group (30.49% vs 79.21%; 0.05). COX multivariate analysis showed that NACT+BT increased PFS by 29% compared with RH treatment, and Positive margin decreased PFS and OS by by 4.7 and 6.87 times, respectively.

Conclusion: Neoadjuvant chemotherapy combined with brachytherapy followed by radical hysterectomy (NACT+BT) can extend PFS, reduce postoperative pathological risk, and postoperative adjuvant pelvic radiotherapy compared to the direct radical hysterectomy (RH).
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http://dx.doi.org/10.3389/fonc.2021.618612DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8023045PMC
March 2021

Comparison of the survival outcomes of laparoscopy versus laparotomy in treatment of early-stage ovarian cancer: a systematic review and meta-analysis.

J Ovarian Res 2021 Mar 16;14(1):45. Epub 2021 Mar 16.

Department of Obstetrics and Gynecology, Peking University International Hospital, Life Park of Zhongguancun, Changping District, Beijing, 102206, China.

Background: Laparoscopy has been widely used for patients with early-stage epithelial ovarian cancer (eEOC). However, there is limited evidence regarding whether survival outcomes of laparoscopy are equivalent to those of laparotomy among patients with eEOC. The result of survival outcomes of laparoscopy is still controversial. The aim of this meta-analysis is to analyze the survival outcomes of laparoscopy versus laparotomy in the treatment of eEOC.

Methods: According to the keywords, Pubmed, Embase, Cochrane Library and Clinicaltrials.gov were searched for studies from January 1994 to January 2021. Studies comparing the efficacy and safety of laparoscopy versus laparotomy for patients with eEOC were assessed for eligibility. Only studies including outcomes of overall survival (OS) were enrolled. The meta-analysis was performed using Stata software (Version 12.0) and Review Manager (Version 5.2).

Results: A total of 6 retrospective non-random studies were included in this meta-analysis. The pooled results indicated that there was no difference between two approaches for patients with eEOC in OS (HR = 0.6, P = 0.446), progression-free survival (PFS) (HR = 0.6, P = 0.137) and upstaging rate (OR = 1.18, P = 0.54). But the recurrence rate of laparoscopic surgery was lower than that of laparotomic surgery (OR = 0.48, P = 0.008).

Conclusions: Laparoscopy and laparotomy appear to provide comparable overall survival and progression-free survival outcomes for patients with eEOC. Further high-quality studies are needed to enhance this statement.
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http://dx.doi.org/10.1186/s13048-021-00793-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7968359PMC
March 2021

Assessment of different ventilation strategies on ventilation performance in immersed tunnels.

Environ Sci Pollut Res Int 2021 Jun 22;28(24):31838-31849. Epub 2021 Feb 22.

School of Civil Engineering, Central South University, Changsha, 410075, People's Republic of China.

Investigations of ventilation in an immersed tunnel have recently drawn greater research attentions; however, analyses on the influence of vent design and tunnel width on ventilation performance have rarely been addressed. For the sake of the security of evacuees in an immersed tunnel fire, the influence of three vent designs and two immersed tunnel widths on mechanical ventilation performance during tunnel fires were numerically investigated using large eddy simulation. The pollutant gas flow characteristics in the tunnel after a fire were analyzed, and the pollutant gas exhaust efficiency based on the mass conservation of carbon monoxide in the smoke was proposed in this study. By comparing the smoke propagation, smoke distribution, and exhaust efficiency between three different vent designs, it was determined that the Top Vent Design has the best smoke exhaust effect, and the Sidewall Vent Design (with an activated vertical smoke screen) has a better smoke exhaust effect than the Sidewall Vent Design. The influences of the tunnel width and heat release rate of the fire on the ventilation effect were also investigated.
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http://dx.doi.org/10.1007/s11356-021-12818-9DOI Listing
June 2021

Peptide OM-LV20 exerts neuroprotective effects against cerebral ischemia/reperfusion injury in rats.

Biochem Biophys Res Commun 2021 01 28;537:36-42. Epub 2020 Dec 28.

Department of Anatomy and Histology & Embryology, Faculty of Basic Medical Science, Kunming Medical University, Kunming, Yunnan, 650500, China. Electronic address:

Ischemia/reperfusion (I/R) is a common injury leading to ischemic stroke. At present, I/R treatment remains limited, highlighting the urgent need for the discovery and development of new protective drugs for brain injury. Here, we investigated the neuroprotective effects of short peptide OM-LV20 previously identified from amphibian against I/R rats. Results showed that intraperitoneal administration of OM-LV20 (20 ng/kg) significantly reduced infarct area formation, improved behavioral abnormalities, and protected cortical and hippocampal neurons against death caused by I/R. Moreover, the underlying molecular mechanism was involved with the regulation of the MAPK and BDNF/AKT signaling pathways, as well as the levels of cyclic adenosine monophosphate, pituitary adenylate cyclase-activating polypeptide receptor, and tryptophan hydroxylase 1. To the best of our knowledge, this research was the first report to describe the neuroprotective effects of an amphibian skin secretion-derived peptide in I/R rats and highlighted OM-LV20 as a promising drug candidate for the development of novel anti-stroke therapies.
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http://dx.doi.org/10.1016/j.bbrc.2020.12.053DOI Listing
January 2021

Prevalence of Depressive Symptoms Among Pregnant and Postpartum Women in China During the COVID-19 Pandemic.

Psychosom Med 2021 05;83(4):345-350

From the Department of Nursing (Bo), Peking Union Medical College Hospital, Beijing; Unit of Psychiatry, Institute of Translational Medicine, Faculty of Health Sciences (Yang, Xiang), University of Macau; Center for Cognition and Brain Sciences (Yang, Xiang), University of Macau, Macao SAR; Department of Psychiatry (Yang), Southern Medical University Nanfang Hospital, Guangdong; Department of Gynaecology and Obstetrics (Chen, M. Zhang, D.Y. Zhang, Y. Li, Y. Li, R. Li), Peking Union Medical College Hospital, Beijing; School of Nursing (Cheung), Hong Kong Polytechnic University, Hong Kong SAR, China; and Department of Psychiatry (Ng), The Melbourne Clinic and St Vincent's Hospital, University of Melbourne, Richmond, Victoria, Australia.

Objective: According to recent studies, the COVID-19 pandemic has been associated with an increased risk of mental health problems across many subpopulations including pregnant and postnatal women. This study examined the prevalence and correlates of depressive symptoms (depression hereafter) in Chinese pregnant and postpartum women during the COVID-19 pandemic.

Methods: This was a multicenter, cross-sectional study comprising 1309 pregnant and postpartum women across 12 provinces in China during the COVID-19 pandemic. Depression was assessed using the nine-item Patient Health Questionnaire. Univariate analyses and multivariate logistic regression analyses were conducted.

Results: The prevalence of depression in pregnant and postpartum women was 27.43% (95% confidence interval [CI] = 25.01%-29.85%). Women who were worried about themselves or their babies being infected with COVID-19 (odds ratio [OR] = 2.562, 95% CI = 1.670-3.929), and those who had delayed regular medical checkups (OR = 2.434, 95% CI = 1.580-3.750) were at higher risk of depression. Compared with those living in central and western parts of China, women living in northern (OR = 0.513, 95% CI = 0.326-0.807) and southeastern parts of China (OR = 0.626, 95% CI = 0.463-0.846) were less likely to have depression.

Conclusions: The COVID-19 pandemic was associated with an increased likelihood of mental health problems among pregnant and postnatal women. Over a quarter of the pregnant and postpartum women in China had depression during the COVID-19 pandemic. Considering the negative health impact of depression, preventive measures, regular mental health screening, and medical checkups are needed with the goal to reduce the risk of depression in this vulnerable population during a pandemic.
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http://dx.doi.org/10.1097/PSY.0000000000000904DOI Listing
May 2021

Oral processing preference affects flavor perception in dark chocolate with added ingredients.

J Food Sci 2021 Jan 16;86(1):177-183. Epub 2020 Dec 16.

Office of the Vice Chancellor, University of Waikato, Hamilton, 3216, New Zealand.

Chocolate has specific rheological behavior during oral processing that delivers its distinct sensory characteristics. When incorporating functional or flavoring ingredients into chocolate, these properties must be maintained to meet consumer expectation. Water-soluble and fat-soluble ingredients have a potential effect on the properties of chocolate; therefore, successfully adding functional supplements in this medium can have challenges. This study investigated the effect of functional or flavoring ingredients on chocolate microstructure, mouthfeel (texture), and flavor release, during oral processing. Participants were classified by their oral processing "pattern": a chewing preference (CP), a sucking preference (SP), and a mixed group who had a preference for both chewing and sucking (MP). Chocolate samples (72% dark chocolate) were prepared with different flavor ingredients (water-soluble: ginger powder and fat-soluble: menthol). Instrumental testing of chocolate viscosity and hardness showed no significant differences in chocolate with low concentrations of added ingredients (0.5% ginger and 0.1% mint), while chocolate with higher concentration (2.5% ginger and 0.5% mint) showed a significant difference compared to standard chocolate. Modified Qualitative Descriptive Analysis (MQDA) tests showed no significant differences in sensory perception of texture between the formulations, or the oral processing behavior groups. There was an impact on flavor perception both from composition and from oral processing behavior. The CP group rated the chocolate with the lowest flavoring concentration as also having the lowest cocoa flavor intensity. Moreover, the MP and SP groups showed a similar perception of cocoa flavor intensity.
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http://dx.doi.org/10.1111/1750-3841.15557DOI Listing
January 2021

Novel scorpion venom peptide HsTx2 ameliorates cerebral ischemic brain injury in rats via the MAPK signaling pathway.

Biochem Biophys Res Commun 2021 01 25;534:442-449. Epub 2020 Nov 25.

Department of Anatomy and Histology and Embryology, Faculty of Basic Medical Science, Kunming Medical University, Kunming, 650500, Yunnan, China. Electronic address:

Ischemic stroke is a severe threat to human health due to its high recurrence, mortality, and disability rates. As such, how to prevent and treat ischemic stroke effectively has become a research hotspot in recent years. Here, we identified a novel peptide, named HsTx2 (AGKKERAGSRRTKIVMLKCIREHGH, 2 861.855 Da), derived from the scorpion Heterometrus spinifer, which showed obvious anti-apoplectic effects in rats with ischemic stroke. Results further demonstrated that HsTx2 significantly reduced formation of infarct area and improved behavioral abnormalities in ischemic stroke rats. These protective effects were likely exerted via activation of the mitogen-activated protein kinase (MAPK) signaling pathway, i.e., up-regulation of phosphorylated ERK1/2 in both rat cerebral cortex and activated microglia (AM); up-regulation of phosphorylated p38 (p-p38) in the cerebral cortex; and inhibition of phosphorylated JNK and p-p38 levels in the AM. In conclusion, this study highlights HsTx2 as a potential neuroprotective agent for stroke.
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http://dx.doi.org/10.1016/j.bbrc.2020.11.062DOI Listing
January 2021

Discovery of a novel short peptide with efficacy in accelerating the healing of skin wounds.

Pharmacol Res 2021 01 19;163:105296. Epub 2020 Nov 19.

Department of Anatomy and Histology & Embryology, Faculty of Basic Medical Science, Kunming Medical University, Kunming, Yunnan, 650500, China. Electronic address:

Despite extensive efforts to develop efficacious therapeutic approaches, the treatment of skin wounds remains a considerable clinical challenge. Existing remedies cannot sufficiently meet current needs, so the discovery of novel pro-healing agents is of growing importance. In the current research, we identified a novel short peptide (named RL-QN15, primary sequence 'QNSYADLWCQFHYMC') from Rana limnocharis skin secretions, which accelerated wound healing in mice. Exploration of the underlying mechanisms showed that RL-QN15 activated the MAPK and Smad signaling pathways, and selectively modulated the secretion of cytokines from macrophages. This resulted in the proliferation and migration of skin cells and dynamic regulation of TGF-β1 and TGF-β3 in wounds, which accelerated re-epithelialization and granulation tissue formation and thus skin regeneration. Moreover, RL-QN15 showed significant therapeutic potency against chronic wounds, skin fibrosis, and oral ulcers. Our results highlight frog skin secretions as a potential treasure trove of bioactive peptides with healing activity. The novel peptide (RL-QN15) identified in this research shows considerable capacity as a candidate for the development of novel pro-healing agents.
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http://dx.doi.org/10.1016/j.phrs.2020.105296DOI Listing
January 2021

Spectral response of large-area luminescent solar concentrators.

Appl Opt 2020 Oct;59(28):8964-8969

Measuring the spectral response (SR) of large-area (>100) luminescent solar concentrators (LSCs) has proven difficult because common laboratory photovoltaic (PV) instruments that offer monochromatic incidence measure devices with limited sizes (typically <50). This report addresses this issue through a method called regional measurements. In this method, large-area LSCs are configured to small surface and edge regions, which are sequentially illuminated and measured, respectively. The measured SRs of large-area LSCs are consistent with those from the conventional method and the Monte Carlo ray-tracing simulation. This method is also applied to analyze scattering effects in the LSCs, showing the relationships of the scattering-induced power gain and power loss to the surface root-mean-squared roughness () of the devices. The results explain why the PV performance of the LSCs can be improved through proper surface scattering treatment.
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http://dx.doi.org/10.1364/AO.403354DOI Listing
October 2020

A novel strategy for enhancing anaerobic biodegradation of an anthraquinone dye reactive blue 19 with resuscitation-promoting factors.

Chemosphere 2021 Jan 17;263:127922. Epub 2020 Aug 17.

College of Geography and Environmental Science, Zhejiang Normal University, Jinhua, 321004, China. Electronic address:

Anaerobic process has been widely applied as a cost-effective method for textile wastewater treatment. However, many bacteria exhibit low metabolic activity in unfavorable conditions due to the entry into a viable but non-culturable (VBNC) state. Thus, in this study, a novel method of using resuscitation-promoting factors (Rpfs), which has been proven to resuscitate and stimulate the growth of VBNC bacteria, is explored to enhance the degradation of the anthraquinone dye reactive blue 19 (RB19) in the anaerobic process. The results show that Rpfs could efficiently prompt RB19 decolorization. Compared to the conventional anaerobic condition, RB19 decolorization efficiency was increased by more than 20% with the Rpf addition. UV-visible spectral and gas chromatograph-mass spectrometry analysis indicate that the aromatic amines structures of RB19 was cleaved. More importantly, the Rpf addition appeared to stimulate and/or enrich some dye-degrading species of the family Peptostreptococcaceae, thus leading to a higher RB19 decolorization efficiency.
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http://dx.doi.org/10.1016/j.chemosphere.2020.127922DOI Listing
January 2021

Antisense oligonucleotides targeting lncRNA AC104041.1 induces antitumor activity through Wnt2B/β-catenin pathway in head and neck squamous cell carcinomas.

Cell Death Dis 2020 08 13;11(8):672. Epub 2020 Aug 13.

The Engineering Research Center of Peptide Drug Discovery and Development, China Pharmaceutical University, Nanjing, 210009, P.R. China.

Long non-coding RNAs (lncRNAs) contribute to the initiation and progression of various tumors, including head and neck squamous carcinoma (HNSCC), which is a common malignancy with high morbidity and low survival rate. However, the mechanism of lncRNAs in HNSCC tumorigenesis remains largely unexplored. In this work, we identified a novel lncRNA AC104041.1 which is highly upregulated and correlated with poor survival in HNSCC patients. Moreover, AC104041.1 overexpression significantly promoted tumor growth and metastasis of HNSCC in vitro and in vivo. Mechanistically, AC104041.1 mainly located in the cytoplasm and could function as ceRNA (competing endogenous RNA) for miR-6817-3p, thereby stabilized Wnt2B, and consequently inducing β-catenin nuclear translocation and activation. Moreover, we demonstrate that salinomycin, which as a highly effective antibiotic in the elimination of cancer stem cells through the Wnt/β-catenin signaling, could enhance the inhibition of tumor growth by antisense oligonucleotides (ASO) targeting AC104041.1 in HNSCC cells and PDXs (patient-derived xenograft) model. Thus, our data provide preclinical evidence to support a novel strategy of ASOs targeting AC104041.1 in combination with salinomycin and may as a beneficial treatment approach for HNSCC.
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http://dx.doi.org/10.1038/s41419-020-02820-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7443144PMC
August 2020

TiO microspheres with cross-linked cyclodextrin coating exhibit improved stability and sustained photocatalytic degradation of bisphenol A in secondary effluent.

Water Res 2020 Sep 28;183:116095. Epub 2020 Jun 28.

Nanosystems Engineering Research Center for Nanotechnology Enabled Water Treatment (NEWT), United States; Department of Civil & Environmental Engineering, Houston, TX, 77005, United States. Electronic address:

Photocatalytic water treatment has significant potential to disinfect and degrade recalcitrant organic pollutants while minimizing the need to add chemicals, but current approaches have poor energy efficiency due, in part, to inefficient utilization of photo-generated reactive oxygen species (ROS). Organic coatings such as cyclodextrin (CD) can adsorb target contaminants and bring them close to the photocatalyst surface to enhance ROS utilization efficiency, but the coatings themselves are susceptible to ROS attack. Here, we report an ROS-resistant fluorinated CD polymer (CDP) that can both adsorb contaminants and resist degradation by ROS, yielding a more efficient material for "trap and zap" water treatment. We produced the CDP through condensation polymerization of β-cyclodextrin and tetrafluoroterephthalonitrile, resulting in a cross-linked, covalently bound CD film that is much more stable than prior approaches involving physi-sorption. We optimized the coating thickness on TiO microspheres to improve the efficiency of contaminant degradation, and found that increasing the CDP content enhanced BPA adsorption but also occluded photocatalytic sites and hindered photocatalytic degradation. The optimum content of CDP was 5% by weight, and this optimal CDP-TiO composition had a BPA adsorption capacity of 36.9 ± 1.0 mg g compared with 24.1 ± 1.1 mg g for CD-coated TiO (CD-TiO) and 21.9 ± 1.5 mg g for bare TiO. CDP-TiO exhibited minimal photoactivity loss after 1000 h of repeated use in DI water under UVA irradiation (365 nm, 3.83 × 10 E Ls), and no release of organic carbon from the coating was detected. Photocatalytic treatment using CDP-TiO only showed a small decrease in BPA removal efficiency in secondary effluent after four 3-h cycles, from 80.2% to 71.7%. In contrast, CD-TiO and P25 removed only 29.8% and 6.2% of BPA after 4 cycles, respectively. Altogether, the CDP-TiO microspheres represent promising materials for potential use in photocatalytic water treatment.
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http://dx.doi.org/10.1016/j.watres.2020.116095DOI Listing
September 2020

Dual metal cations coated magnetic mesoporous silica probe for highly selective capture of endogenous phosphopeptides in biological samples.

Mikrochim Acta 2020 06 22;187(7):400. Epub 2020 Jun 22.

Department of Chemistry, Department of Gastroenterology and Hepatology, Zhongshan Hospital, Institutes of Biomedical Sciences, Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai, 200433, China.

For the first time, dual metal ions (Ti-Zr) were successfully modified into the channel of magnetic mesoporous silica to obtain an affinity probe for highly selective capture of endogenous phosphopeptides in biological samples. The newly prepared [email protected]@Ti-Zr composites possessed the advantages of ordered mesoporous channels, superparamagnetism, and enhanced affinity properties of dual metal ions of Ti and Zr. The phosphopeptide enrichment efficiency of the [email protected]@Ti-Zr composite was investigated, and the result indicated an ultrahigh size exclusive ability (weight ratio of β-casein tryptic digests, BSA, and α-casein protein reached up to 1:1000:1000). Compared to magnetic affinity probes with single metal ions ([email protected]@Ti, [email protected]@Zr), the composite possessed stronger specificity, higher sensitivity, and better efficiency; and more importantly, it showed much enhanced enrichment ability towards both mono- and multi-phosphorylated peptides. Additionally, by utilizing the [email protected]@Ti-Zr affinity probe, a total number of 104 endogenous phosphopeptides including 95 mono-phosphopeptides and 9 multi-phosphopeptides were captured and identified from human saliva, indicating the great potential for the application of the novel probe for the peptidome analysis in the future. Graphic abstract.
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http://dx.doi.org/10.1007/s00604-020-04323-6DOI Listing
June 2020

RDP3, A Novel Antigout Peptide Derived from Water Extract of Rice.

J Agric Food Chem 2020 Jul 24;68(27):7143-7151. Epub 2020 Jun 24.

Department of Anatomy and Histology & Embryology, Faculty of Basic Medical Science, Kunming Medical University, Kunming 650500, Yunnan, China.

Gout and hyperuricemia can seriously affect the quality of life; at present, however, existing medicines are unable to meet all clinical needs. In the current study, a novel peptide (i.e., rice-derived-peptide-3 (RDP3), AAAAMAGPK-NH, 785.97 Da) in water extract obtained from shelled fruits was identified. Testing revealed that RDP3 (minimum effective concentration 100 μg/kg) did not show both hemolytic and acute toxicity, and reduced uric acid levels in the serum of hyperuricemic mice by inhibiting xanthine oxidase activity and decreasing urate transporter 1 expression. RDP3 also alleviated renal injury in hyperuricemic mice by decreasing NLRP3 inflammasome expression. Furthermore, RDP3 alleviated formalin-induced paw pain and reduced monosodium urate crystal-induced paw swelling and inflammatory factors in mice. Thus, this newly identified peptide reduced uric acid levels and renal damage in hyperuricemic mice and showed anti-inflammatory and analgesic activities, indicating the potential of RDP3 as an antigout medicine candidate.
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http://dx.doi.org/10.1021/acs.jafc.0c02535DOI Listing
July 2020

An embodied energy perspective of urban economy: A three-scale analysis for Beijing 2002-2012 with headquarter effect.

Sci Total Environ 2020 Aug 6;732:139097. Epub 2020 May 6.

HAAM Group, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.

As the typical characteristic of globalization, large-scale agglomeration of headquarters in urban economies exerts extensive cross-border trade links, and inevitably generates energy use outside their boundary. Therefore, studies about urban economies' energy use profiles should pay special attention to the tremendous energy transfers embodied in their trade connections along the whole supply chain. In this regard, a three-scale input-output model which distinguishes local, domestic and foreign activities is devised to reflect cross border embodied energy perspective for urban economies, with an intensive case study for Beijing during 2002-2012. The results show that domestic imports dominate Beijing's total embodied energy use, while local energy exploitation accounts for less than one-tenths of the final use. Regarding to energy use embodied in trade, headquarter effect contributes significantly to the rapid growth of embodied energy inflows and outflows. Embodied energy transfers induced by headquarter effect almost doubled in the case period. Different industries show distinct embodied energy redistribution evolution characteristics. Moreover, the complete source-to-sink budget is constructed, implying that coal use still dominates Beijing's total embodied energy inputs. Analysis in this study highlights the importance to consider the impacts of headquarter effect on Beijing's embodied energy use and redistribution pattern, pointing the potential room for policy implications aimed to realize collective and inclusive governance of global energy supply chain.
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http://dx.doi.org/10.1016/j.scitotenv.2020.139097DOI Listing
August 2020
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