Publications by authors named "Yijun Wu"

48 Publications

Ultrasensitive detection of circulating tumour DNA via deep methylation sequencing aided by machine learning.

Nat Biomed Eng 2021 Jun 15;5(6):586-599. Epub 2021 Jun 15.

Department of Thoracic Surgery, Shanghai Chest Hospital, Shanghai, China.

The low abundance of circulating tumour DNA (ctDNA) in plasma samples makes the analysis of ctDNA biomarkers for the detection or monitoring of early-stage cancers challenging. Here we show that deep methylation sequencing aided by a machine-learning classifier of methylation patterns enables the detection of tumour-derived signals at dilution factors as low as 1 in 10,000. For a total of 308 patients with surgery-resectable lung cancer and 261 age- and sex-matched non-cancer control individuals recruited from two hospitals, the assay detected 52-81% of the patients at disease stages IA to III with a specificity of 96% (95% confidence interval (CI) 93-98%). In a subgroup of 115 individuals, the assay identified, at 100% specificity (95% CI 91-100%), nearly twice as many patients with cancer as those identified by ultradeep mutation sequencing analysis. The low amounts of ctDNA permitted by machine-learning-aided deep methylation sequencing could provide advantages in cancer screening and the assessment of treatment efficacy.
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http://dx.doi.org/10.1038/s41551-021-00746-5DOI Listing
June 2021

A20 functions as a negative regulator in macrophage for DSS-induced colitis.

Int Immunopharmacol 2021 Aug 29;97:107804. Epub 2021 May 29.

Department of Gastroenterology, The First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe East Road, Zhengzhou 450052, Henan, China. Electronic address:

The function of A20 as a deubiquitinating enzyme in inflammatory diseases and autoimmune diseases has been reported, we therefore aimed to investigate the potential effects of A20 in macrophages and dextran sodium sulfate (DSS)-induced colitis mouse model. Colitis mouse model was induced by DSS treatment. Tnfaip3 mice were crossed with Lyz2-Cre mice to generate A20 myeloid cell-conditional knockout mice. The expression levels of indicated cytokines were analyzed by quantitative reverse transcriptase real-time polymerase chain reaction and enzyme-linked immunosorbent assay. Phosphorylated and total protein levels in nuclear factor kappa B (NF-κB) signaling pathway were detected by Western blot. In the bone marrow of mice, A20 deficiency did not affect macrophage development. In bone marrow-derived macrophages (BMDMs) after lipopolysaccharide (LPS) treatment, A20 deficiency enhanced pro-inflammatory cytokine expression. A20 deficiency in macrophages led to severe symptoms of DSS-induced colitis in mice. A20 deficiency enhanced the NF-κB signaling pathway activity in BMDMs. The effects of A20 deficiency in DSS-induced colitis were suppressed by NF-κB pathway inhibition. A20/inhibitor of NF-κB kinase 2 (IKKβ)-double knockout mice were resistant to DSS-induced colitis. A20 suppresses pro-inflammatory cytokine expression in macrophages through the NF-κB signal pathway and alleviates the pathogenesis of DSS-induced colitis in mice.
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http://dx.doi.org/10.1016/j.intimp.2021.107804DOI Listing
August 2021

Mechanism of circADD2 as ceRNA in Childhood Acute Lymphoblastic Leukemia.

Front Cell Dev Biol 2021 13;9:639910. Epub 2021 May 13.

Department of Hematology and Oncology, Children's Hospital of Nanjing Medical University, Nanjing, China.

Acute lymphocytic leukemia (ALL) is the most common malignant tumor in children. Increasing evidence suggests that circular RNAs (circRNAs) play critical regulatory roles in tumor biology. However, the expression patterns and roles of circRNAs in childhood acute lymphoblastic leukemia (ALL) remain largely unknown. circADD2 was selected by microarray assay and confirmed by qRT-PCR; effects of circADD2 were determined by CCK-8 and flow cytometry; while mice subcutaneous tumor model was designed for analysis. RNA immunoprecipitation and dual-luciferase assay were applied for mechanistic study. Protein levels were examined by Western blot assay. circADD2 was down-regulated in ALL tissues and cell lines. Overexpression of circADD2 inhibited cell proliferation and promoted apoptosis both and . Briefly, circADD2 could directly sponge miR-149-5p, and the level of AKT2, a target gene of miR-149-5p, was downregulated by circADD2. circADD2, as a tumor suppressor in ALL, can sponge miR-149-5p, and may serve as a potential biomarker for the diagnosis or treatment of ALL.
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http://dx.doi.org/10.3389/fcell.2021.639910DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155473PMC
May 2021

Outcome of Non-small Cell Lung Cancer Patients With N3 Stage: Survival Analysis of Propensity Score Matching With a Validated Predictive Nomogram.

Front Surg 2021 30;8:666332. Epub 2021 Apr 30.

Department of Radiation Oncology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.

N3-positive non-small cell lung cancer (NSCLC) is usually regarded as inoperable. There were very few studies that focused on N3-NSCLC patients. This study aims to analyze prognosis of NSCLC patients with N3 disease and provides retrospective indications. NSCLC patients staged as N3 were retrospectively reviewed from the Surveillance, Epidemiology, and End Results database. Univariate and multivariate Cox regression were used for identifying prognostic factors. The selected predictive parameters by the least absolute shrinkage and selection operator (LASSO) regression were used to develop predictive nomogram models for overall survival (OS) and lung cancer-specific survival (CSS). The C-index values were calculated to assess the models' predictive ability, while calibration curves were plotted to evaluate the agreement between the predicted and the actual survival. Survival curves were plotted by Kaplan-Meier method and were compared by log-rank test. Propensity score matching (PSM) was used to balance the baseline characteristics between treatment groups. A total of 24,747 N3-NSCLC patients were enrolled. The 1-, 3-, and 5-year OS rates were 35.8, 6.8, and 1.7%, respectively, while the corresponding CSS rates was 36.6, 6.9, and 1.8%, respectively. The nomogram models were developed using 11 significant prognostic parameters, including age, sex, race, histology, stage, T stage, bone, brain and liver metastases, surgery, and chemotherapy. Both of them demonstrated great predictive ability and performed well in the calibration curves. After PSM, patients receiving surgery demonstrated significantly better survival than those who did not. Besides, there was no significant difference of survival between patients receiving chemotherapy with and without radiotherapy. The nomogram models for predicting survival outcome of N3-NSCLC patients can be clinically used. Surgery may be beneficial to the survival for these patients, while radiotherapy may not have additional survival benefits in patients receiving chemotherapy.
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http://dx.doi.org/10.3389/fsurg.2021.666332DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120028PMC
April 2021

Comprehensive exploration of tumor mutational burden and immune infiltration in diffuse glioma.

Int Immunopharmacol 2021 Jul 10;96:107610. Epub 2021 Apr 10.

Department of Radiation Oncology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. Electronic address:

Background: Immune checkpoint inhibitors (ICIs) have been used as a novel treatment for diffuse gliomas, but the efficacy varies with patients, which may be associated with the tumor mutational burden (TMB) and immune infiltration. We aimed to explore the relationship between the two and their impacts on the prognosis.

Methods: The data of the training set were downloaded from The Cancer Genome Atlas (TCGA). "DESeq2" R package was used for differential analysis and identification of differentially expressed genes (DEGs). A gene risk score model was constructed based on DEGs, and a nomogram was developed combined with clinical features. With the CIBERSORT algorithm, the relationship between TMB and immune infiltration was analyzed, and an immune risk score model was constructed. Two models were verification in the validation set downloaded from the Chinese Glioma Genome Atlas (CGGA).

Results: Higher TMB was related to worse prognosis, older age, higher grade, and higher immune checkpoint expression. The gene risk score model was constructed based on BIRC5, SAA1, and TNFRSF11B, and their expressions were all negatively correlated with prognosis. The nomogram was developed combined with age and grade. The immune risk score model was constructed based on M0 macrophages, neutrophils, naïve CD4 T cells, and activated mast cells. The proportions of the first two were higher in the high-TMB group and correlated with worse prognosis, while the latter two were precisely opposite.

Conclusions: In diffuse gliomas, TMB was negatively correlated with prognosis. The association of immune infiltration with TMB and prognosis varied with the type of immune cells. The nomogram and risk score models can accurately predict prognosis. The results can help identify patients suitable for ICIs and potential therapeutic targets, thus improve the treatment of diffuse gliomas.
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http://dx.doi.org/10.1016/j.intimp.2021.107610DOI Listing
July 2021

Predicting occult lymph node metastasis by nomogram in patients with lung adenocarcinoma ≤2 cm.

Future Oncol 2021 Jun 31;17(16):2005-2013. Epub 2021 Mar 31.

Department of Thoracic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, China.

Previous researches had not proposed any prediction models for occult lymph node metastasis (OLNM). Considering the occurrence of OLNM and the importance of OLNM management, we aimed to develop a nomogram to predict OLNM of patients with lung adenocarcinoma ≤2 cm. Characteristics of patients with lung adenocarcinoma of ≤2 cm diameter at the Peking Union Medical College Hospital were retrospectively reviewed. Univariate and multivariate logistic regressions were performed. A nomogram model was developed. The concordance index (C-index) and calibration and decision curves were used to evaluate the predictive ability. A total of 473 patients were enrolled, with an OLNM incidence of 7.4%. Four factors were selected as risk factors. The model had a C-index of 0.932. Calibration and decision curves were determined. Patients with pure ground-glass opacity (pGGO) or noninvasive adenocarcinoma have significantly lower risk of OLNM. SUV, CEA, micropapillary and solid component were identified as independent risk factors. The nomogram model was effective in predicting OLNM preoperatively.
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http://dx.doi.org/10.2217/fon-2020-0905DOI Listing
June 2021

To Predict the Length of Hospital Stay After Total Knee Arthroplasty in an Orthopedic Center in China: The Use of Machine Learning Algorithms.

Front Surg 2021 11;8:606038. Epub 2021 Mar 11.

Department of Orthopaedic Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.

Total knee arthroplasty (TKA) is widely performed to improve mobility and quality of life for symptomatic knee osteoarthritis patients. The accurate prediction of patients' length of hospital stay (LOS) can help clinicians for rehabilitation decision-making and bed assignment planning, which thus makes full use of medical resources. Clinical characteristics were retrospectively collected from 1,298 patients who received TKA. A total of 36 variables were included to develop predictive models for LOS by multiple machine learning (ML) algorithms. The models were evaluated by the receiver operating characteristic (ROC) curve for predictive performance and decision curve analysis (DCA) for clinical values. A feature selection approach was used to identify optimal predictive factors. The areas under the ROC curve (AUCs) of the nine models ranged from 0.710 to 0.766. All the ML-based models performed better than models using conventional statistical methods in both ROC curves and decision curves. The random forest classifier (RFC) model with 10 variables introduced was identified as the best predictive model. The feature selection indicated the top five predictors: tourniquet time, distal femoral osteotomy thickness, osteoporosis, tibia component size, and post-operative values of Hb within 24 h. By analyzing clinical characteristics, it is feasible to develop ML-based models for the preoperative prediction of LOS for patients who received TKA, and the RFC model performed the best.
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http://dx.doi.org/10.3389/fsurg.2021.606038DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7990876PMC
March 2021

Role of chemotherapy for survival in patients with second primary non-small cell lung cancer.

Thorac Cancer 2021 02 9;12(4):426-443. Epub 2020 Dec 9.

Department of Thoracic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.

Background: The therapeutic effect of chemotherapy is still unclear for clinical usage among second primary non-small cell lung cancer (NSCLC) patients. The aim of this study was to verify the therapeutic effect of chemotherapy and identify the prognostic factors among patients who had received chemotherapy for second primary NSCLC.

Methods: A retrospective cohort was constructed based on the Surveillance, Epidemiology and End Results (SEER) database. Through least absolute shrinkage and selection operator regression, univariate Cox and multivariate Cox regression, we identified the prognostic factors among clinicopathological features. Propensity score matching analysis was used to verify the therapeutic effect of chemotherapy. Survival curves were plotted among the subgroups of the selected factors. We further selected clinicopathological features that would affect the prognosis among patients who had received chemotherapy through a similar process.

Results: A total of 769 patients were enrolled to verify the therapeutic value of chemotherapy for second primary lung cancer. Significant differences were observed between the chemotherapy and nonchemotherapy group for cancer-specific survival. 215 patients who had received chemotherapy were analyzed to identify the factors that might influence outcome on the therapeutic effect of chemotherapy. Age, tumor size, histology and treatment were selected as significant factors.

Conclusions: The therapeutic effect of chemotherapy for second primary NSCLC was found to be significant. Age, tumor size and histology were significant prognostic factors among patients who had received chemotherapy for second primary NSCLC.

Key Points: Significant findings of the study A significant therapeutic effect of chemotherapy for second primary non-small cell lung cancer was proven through univariate Cox regression and propensity score matching analysis. Prognostic factors for second primary non-small cell lung cancer patients who had received chemotherapy. What this study adds Chemotherapy could be applied in clinical practice as an additional therapeutic method for second primary non-small cell lung cancer patients. We selected prognostic factors for patients who had received chemotherapy to identify patients who were appropriate for chemotherapy.
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http://dx.doi.org/10.1111/1759-7714.13762DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7882385PMC
February 2021

Papillary thyroid carcinoma presenting as a functioning thyroid nodule: report of 2 rare cases.

Authors:
Liang Hu Yijun Wu

Int J Clin Exp Pathol 2020 1;13(11):2895-2906. Epub 2020 Nov 1.

Department of Thyroid Surgery, The First Hospital Affiliated to Zhejiang University, School of Medicine No. 79 Qingchun Road, Hangzhou 310000, P. R. China.

Introduction: Autonomously functioning thyroid nodules (AFTNs) are generally benign, whereas papillary thyroid carcinomas (PTCs) are mostly non-functioning. Graves' disease (GD) is the most common cause of hyperthyroidism (HD), followed by hyperfunctional adenoma or Plummer's disease. GD with AFTNs is called Marine-Lenhart syndrome, a relatively rare syndrome. In clinical practice, the presence of HD, AFTNs and PTC at the same time is extremely rare.

Case Presentation: Case 1: A 55-year-old middle-aged woman with a preoperative diagnosis of GD and HD with right AFTNs. Case 2: A 43-year-old middle-aged woman with a preoperative diagnosis of non-GD and HD with right AFTNs and right PTC. Case 1: Histology showed a 4 cm adenoma with a 1.0 cm PTC in the right lobe and a 0.3 cm PTC in the left lobe. The rest of the thyroid showed typical pathologic GD changes. The postoperative diagnosis was atypical Marine-Lenhart syndrome with bilateral PTC. Case 2: Histology showed a 0.4 cm PTC surrounded by nodular goiter. The postoperative diagnosis was toxic nodular goiter with PTC.

Conclusion: This paper covers the relationships among PTC, HD and AFTNs, explains some common and uncommon clinical diagnoses, and reports two rare cases with these three diagnoses. Our ultimate purpose is to remind doctors that when handling nodules or HD, PTC as a diagnosis cannot be excluded. Instead, it is better to perform total or near-total thyroidectomy and intraoperative frozen biopsy or preoperative biopsy examinations to avoid omitting PTC, which needs reoperation.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7716118PMC
November 2020

Prognostic outcome after second primary lung cancer in patients with previously treated lung cancer by radiotherapy.

J Thorac Dis 2020 Oct;12(10):5376-5386

Department of Radiation Oncology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.

Background: Second primary lung cancer (SPLC) occurs not rarely in recent years. The effect of radiotherapy on SPLC remains unclear. This study aims to explore the survival outcome of SPLC patients with clinical stage T1 lung cancer previously treated with radiotherapy.

Methods: A total of 705 SPLC patients that previously underwent radiotherapy for first primary lung cancer (FPLC) were identified from the Surveillance, Epidemiology, and End Results (SEER) database between 2004 and 2016. Univariate and multivariate Cox regression analyses were performed to find prognostic factors. The survival outcomes were plotted using Kaplan-Meier (KM) method and compared by log-rank test. Additionally, propensity score matching (PSM) analyses were used to compare overall survival (OS) and lung cancer-specific survival (CSS) between radiotherapy and other treatment groups for SPLC.

Results: According to Cox analyses, age >62 years [hazard ratio (HR): 1.48, 95% confidence interval (CI): 1.10-1.99; P=0.010], SPLC tumor size >1 cm (HR: 1.95, 95% CI: 1.51-2.53; P<0.001), and treatments for SPLC as chemotherapy (HR: 1.39, 95% CI: 1.13-1.71; P=0.002), no surgery (HR: 2.00, 95% CI: 1.34-2.98; P=0.001) and no radiotherapy (HR: 1.73, 95% CI: 1.39-2.15; P<0.001) independently indicated worse survival. After PSM, patients treated with radiotherapy for SPLC had significantly better OS and CSS than the none-treatment (OS: P=0.004; CSS: P<0.001), chemotherapy (P<0.001) or radiotherapy plus chemotherapy (OS: P=0.032; CSS: P=0.008) groups, but demonstrated a worse OS than the surgery group (P=0.034).

Conclusions: Surgery may be more beneficial to survival than radiotherapy and chemotherapy and should be considered first if possible. When patients cannot tolerate surgery, radiotherapy can be an effective alternative.
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http://dx.doi.org/10.21037/jtd-20-2024DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7656431PMC
October 2020

Metastatic Patterns of Mediastinal Lymph Nodes in Small-Size Non-small Cell Lung Cancer (T1b).

Front Surg 2020 22;7:580203. Epub 2020 Sep 22.

Department of Thoracic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.

Lymph node metastasis (LNM) status is critical to the treatment. Fewer studies has focused on LNM in patients with small-size non-small cell lung cancer (NSCLC). This study aims to investigate clinicopathological characteristics associated with skip N2 (SN2) and non-skip N2 (NSN2) metastasis, and their metastatic patterns in NSCLC with tumor size of 1-2 cm. We reviewed the records of NSCLC patients with tumor size of 1-2 cm who underwent lobectomy with systematic lymph node dissection (LND) between January 2013 and June 2019. Clinical, radiographical, and pathological characteristics were compared among N1, SN2, and NSN2 groups. Metastatic patterns of mediastinal lymph node were analyzed based on final pathology. A total of 63 NSCLC patients with tumor size of 1-2 cm were staged as pN2, including 25 (39.7%) SN2 and 38 (60.3%) NSN2. The incidence rates of SN2 and NSN2 were 2.8% (25/884) and 4.3% (38/884), respectively. For all clinicopathological characteristics, no significant difference was observed among the groups of N1, SN2, and NSN2. For the tumor located in each lobe, specific nodal drainage stations were identified: 2R/4R for right upper lobe; 2R/4R and subcarinal node (#7) for right middle lobe and right lower lobe; 4L and subaortic node (#5) for left upper lobe; #7 for left lower lobe. However, there were still a few patients (10.9%, 5/46) had the involvement of lower zone for tumors of upper lobe and the involvement of upper zone for lower lobe. SN2 occurs frequently in patients with small-size NSCLC. Whether lobe-specific selective LND is suitable for all small-size patients deserves more studies to confirm. Surgeons should be more careful when performing selective LND for tumors located in the lower and upper lobes.
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http://dx.doi.org/10.3389/fsurg.2020.580203DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7536402PMC
September 2020

Machine Learning Algorithms for the Prediction of Central Lymph Node Metastasis in Patients With Papillary Thyroid Cancer.

Front Endocrinol (Lausanne) 2020 21;11:577537. Epub 2020 Oct 21.

Department of General Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: Central lymph node metastasis (CLNM) occurs frequently in patients with papillary thyroid cancer (PTC), but performing prophylactic central lymph node dissection is still controversial. There are no reliable models for predicting CLNM. This study aimed to develop predictive models for CLNM by machine learning (ML) algorithms.

Methods: Patients with PTC who underwent initial thyroid resection at our hospital between January 2018 and December 2019 were enrolled. A total of 22 variables, including clinical characteristics and ultrasonography (US) features, were used for conventional univariate and multivariate analysis and to construct ML-based models. A 5-fold cross validation strategy was used for validation and a feature selection approach was applied to identify risk factors.

Results: The areas under the receiver operating characteristic curve (AUC) of 7 models ranged from 0.680 to 0.731. All models performed significantly better than US (AUC=0.623) in predicting CLNM (P<0.05). In decision curve, most of the models also performed better than US. The gradient boosting decision tree model with 7 variables was identified as the best model because of its best performance in both ROC (AUC=0.731) and decision curves. Based on multivariate analysis and feature selection, young age, male sex, low serum thyroid peroxidase antibody and US features such as suspected lymph nodes, microcalcification and tumor size > 1.1 cm were the most contributing predictors for CLNM.

Conclusions: It is feasible to develop predictive models of CLNM in PTC patients by incorporating clinical characteristics and US features. The ML algorithm may be a useful tool for the prediction of lymph node metastasis in thyroid cancer.
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http://dx.doi.org/10.3389/fendo.2020.577537DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7609926PMC
June 2021

The clinical value of Delphian lymph node metastasis in papillary thyroid carcinoma.

Asian J Surg 2020 Dec 26;43(12):1180-1181. Epub 2020 Sep 26.

Department of Thyroid Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China. Electronic address:

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http://dx.doi.org/10.1016/j.asjsur.2020.09.008DOI Listing
December 2020

Prognostic study for survival outcome following the treatment of second primary lung cancer in patients with previously resected non-small cell lung cancer.

Thorac Cancer 2020 10 26;11(10):2840-2851. Epub 2020 Aug 26.

Department of Thoracic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.

Background: Patients who have previously undergone surgical resection of initial primary lung cancer (IPLC) are at high risk of developing second primary lung cancer (SPLC). There are still no standard treatments for SPLC. This study aimed to identify the prognostic factors and compare survival between the different SPLC treatment groups.

Methods: SPLC patients in the Surveillance, Epidemiology, and End Results (SEER) database between 2007 and 2016 were retrospectively reviewed. Prognostic factors for SPLC were identified, using the least absolute shrinkage and selection operator (LASSO) regression and univariate Cox analysis to select variables for multivariate Cox analysis. Kaplan-Meier method plus log-rank test and restricted mean survival time (RMST) were used to compare survival outcome.

Results: A total of 665 SPLC patients were finally enrolled into the study. Multivariate Cox regression analysis revealed that male vs. female (HR = 1.82, 95% CI: 1.29-2.59, P = 0.001), tumor size of SPLC ≥1 cm vs. <1 cm (HR = 1.80, 96% CI: 1.07-3.02, P = 1.028), IPLC characteristics of squamous cell carcinoma vs. adenocarcinoma (HR = 1.89, 95% CI: 1.17-3.04, P = 0.009), clinical stage II vs. stage I (HR = 2.60, 95% CI: 1.08-6.27, P = 0.033), and T2 stage vs. T1 stage (HR = 1.68, 95% CI: 1.04-2.72, P = 0.034) indicated worse survival. SPLC patients demonstrated a five-year survival rate of 68.6% and a five-year RMST of 49.4 months. The choice of surgical procedure (wedge resection, segmentectomy and lobectomy) for both IPLC and SPLC had no significant effect on prognosis (P > 0.05). Patients that received radiotherapy for SPLC also demonstrated similar survival when compared with those that underwent surgery (P > 0.05).

Conclusions: Radiotherapy and sublobar resection can be considered reasonable alternative treatments for SPLC, especially when patients are unable to tolerate lobectomy.
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http://dx.doi.org/10.1111/1759-7714.13610DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7529572PMC
October 2020

An Externally-Validated Dynamic Nomogram Based on Clinicopathological Characteristics for Evaluating the Risk of Lymph Node Metastasis in Small-Size Non-small Cell Lung Cancer.

Front Oncol 2020 7;10:1322. Epub 2020 Aug 7.

Department of Thoracic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Lymph node metastasis (LNM) status is of key importance for the decision-making on treatment and survival prediction. There is no reliable method to precisely evaluate the risk of LNM in NSCLC patients. This study aims to develop and validate a dynamic nomogram to evaluate the risk of LNM in small-size NSCLC. The NSCLC ≤ 2 cm patients who underwent initial pulmonary surgery were retrospectively reviewed and randomly divided into a training cohort and a validation cohort as a ratio of 7:3. The training cohort was used for the least absolute shrinkage and selection operator (LASSO) regression to select optimal variables. Based on variables selected, the logistic regression models were developed, and were compared by areas under the receiver operating characteristic curve (AUCs) and decision curve analysis (DCA). The optimal model was used to plot a dynamic nomogram for calculating the risk of LNM and was internally and externally well-validated by calibration curves. LNM was observed in 12.0% (83/774) of the training cohort and 10.1% (33/328) of the validation cohort ( = 0.743). The optimal model was used to plot a nomogram with six variables incorporated, including tumor size, carcinoembryonic antigen, imaging density, pathological type (adenocarcinoma or non-adenocarcinoma), lymphovascular invasion, and pleural invasion. The nomogram model showed excellent discrimination (AUC = 0.895 vs. 0.931) and great calibration in both the training and validation cohorts. At the threshold probability of 0-0.8, our nomogram adds more net benefits than the treat-none and treat-all lines in the decision curve. This study firstly developed a cost-efficient dynamic nomogram to precisely and expediently evaluate the risk of LNM in small-size NSCLC and would be helpful for clinicians in decision-making.
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http://dx.doi.org/10.3389/fonc.2020.01322DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7426394PMC
August 2020

ABIN1 alleviates inflammatory responses and colitis facilitating A20 activity.

Ther Adv Chronic Dis 2020 30;11:2040622320944782. Epub 2020 Jul 30.

Department of Gastroenterology, the First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe East Road, Zhengzhou, Henan 450052, China.

Background: Macrophages-mediated inflammation is involved in the progress of colitis. The present study aims to explore the roles of A20-binding inhibitor of NF-κB (ABIN1) in the macrophages and its underlying mechanisms.

Methods: ABIN1 myeloid cell-conditional transgenic mice were established and genotyped by PCR and immunoblotting assays. Tumor necrosis factor (TNF)-α was applied to pre-treat bone marrow-derived macrophages (BMDMs) in the presence of lipopolysaccharide. The mRNA and protein levels of pro-inflammatory cytokines were determined by qRT-PCR and ELISA, respectively. Dextran sulfate sodium (DSS)-induced colitis was established to determine the effects of ABIN1 on the survival time, body weight, colon length, and colon histopathological changes. Western blotting was applied to determine the expressions of signaling proteins.

Results: ABIN1 overexpression did not affect cell populations of macrophages and neutrophils in mice. Its overexpression reduced the productions of pro-inflammatory cytokines in BMDMs and ameliorated survival rate and colitis symptoms in the DSS-induced mouse model. The underlying mechanisms revealed that ABIN1 impaired macrophages-mediated inflammatory responses, in part by regulating the NF-κB signal pathway, and its ameliorated effects on the symptoms of DSS-induced colitis were associated with A20/tumor necrosis factor α-induced protein 3 (TNFAIP3).

Conclusion: ABIN1 attenuated inflammatory responses and colitis by regulating A20/TNFAIP3 activities.
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http://dx.doi.org/10.1177/2040622320944782DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7418473PMC
July 2020

The Value of TTPVI in Prediction of Microvascular Invasion in Hepatocellular Carcinoma.

Cancer Manag Res 2020 2;12:4097-4105. Epub 2020 Jun 2.

Department of Pathology, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, People's Republic of China.

Purpose: The objective of our study was to evaluate the value of two-trait predictor of venous invasion (TTPVI) in the prediction of pathological microvascular invasion (pMVI) in patients with hepatocellular carcinoma (HCC) from preoperative computed tomography (CT) and magnetic resonance (MR).

Methods: A total of 128 preoperative patients with findings of HCC were enrolled. Tumor size, tumor margins, tumor capsule, peritumoral enhancement, and TTPVI was assessed on preoperative CT and MRI images. Histopathological features were reviewed: pathological tumor size, tumor differentiation, pMVI along with alpha-fetoprotein level (AFP). Significant imaging findings and histopathological features were determined with univariate and multivariate logistic regression analysis.

Results: Univariate analysis revealed that tumor size (<0.01), AFP level (=0.043), tumor differentiation (<0.01), peritumoral enhancement (=0.003), pathological tumor size (<0.01), tumor margins (<0.01) on CT and MRI, and TTPVI (<0.01) showed statistically significant associations with pMVI. In multivariate logistic regression analysis, tumor size (odds ratio [OR] = 1.294; 95% confidence interval [CI]: 1.155, 1.451; < 0.001), tumor differentiation (odds ratio [OR] =1.384; 95% confidence interval [CI]: 1.224, 1.564; < 0.001), and TTPVI (odds ratio [OR] = 4.802; 95% confidence interval [CI]: 1.037, 22.233; =0.045) were significant independent predictors of pMVI. Using 5.8 as the threshold for size, one could obtain an area-under-curve (AUC) of 0.793, 95% confidence interval [CI]: 0.715 to 0.857.

Conclusion: Tumor size, tumor differentiation, and TTPVI depicted in preoperative CT and MRI had a statistically significant correlation with pMVI. Hence, TTPVI detected on CT and MRI may be predictive of pMVI in HCC cases.
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http://dx.doi.org/10.2147/CMAR.S245475DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7276193PMC
June 2020

Preoperative Prediction of Lymph Node Metastasis in Patients With Early-T-Stage Non-small Cell Lung Cancer by Machine Learning Algorithms.

Front Oncol 2020 13;10:743. Epub 2020 May 13.

Department of Thoracic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Lymph node metastasis (LNM) is difficult to precisely predict before surgery in patients with early-T-stage non-small cell lung cancer (NSCLC). This study aimed to develop machine learning (ML)-based predictive models for LNM. Clinical characteristics and imaging features were retrospectively collected from 1,102 NSCLC ≤ 2 cm patients. A total of 23 variables were included to develop predictive models for LNM by multiple ML algorithms. The models were evaluated by the receiver operating characteristic (ROC) curve for predictive performance and decision curve analysis (DCA) for clinical values. A feature selection approach was used to identify optimal predictive factors. The areas under the ROC curve (AUCs) of the 8 models ranged from 0.784 to 0.899. Some ML-based models performed better than models using conventional statistical methods in both ROC curves and decision curves. The random forest classifier (RFC) model with 9 variables introduced was identified as the best predictive model. The feature selection indicated the top five predictors were tumor size, imaging density, carcinoembryonic antigen (CEA), maximal standardized uptake value (SUV), and age. By incorporating clinical characteristics and radiographical features, it is feasible to develop ML-based models for the preoperative prediction of LNM in early-T-stage NSCLC, and the RFC model performed best.
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http://dx.doi.org/10.3389/fonc.2020.00743DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7237747PMC
May 2020

Publication Landscape Analysis on Gliomas: How Much Has Been Done in the Past 25 Years?

Front Oncol 2019 17;9:1463. Epub 2020 Jan 17.

Department of Neurosurgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.

The body of glioma-related literature has grown significantly over the past 25 years. Despite this growth in the amount of published research, gliomas remain one of the most intransigent cancers. The purpose of this study was to analyze the landscape of glioma-related research over the past 25 years using machine learning and text analysis. In April 2019, we downloaded glioma-related publications indexed in PubMed between 1994 and 2018. We used Python to extract the title, publication date, MeSH terms, and abstract from the metadata of each publication for bibliometric assessment. Latent Dirichlet allocation (LDA) was applied to the abstracts to identify publications' research topics with greater specificity. We identified and analyzed a total of 52,625 publications in our study. We found that research on prognosis and the treatment of glioblastoma increased the most in terms of volume and rate of publications over the past 25 years. However, publications regarding clinical trials accounted for <5% of all publications considered in this study. The current research landscape covers clinical, pre-clinical, biological, and technical aspects of glioblastoma; at present, researchers appear to be less concerned with glioblastoma's psychological effects or patients' end-of-life care. Publication of glioma-related research has expanded rapidly over the past 25 years. Common topics include the disease's molecular background, patients' survival, and treatment outcomes; more research needs to be done on the psychological aspects of glioblastoma and end-of-life care.
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http://dx.doi.org/10.3389/fonc.2019.01463DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6988829PMC
January 2020

Expression and prognostic analyses of , and in human non-small cell lung cancer.

PeerJ 2019 20;7:e8299. Epub 2019 Dec 20.

Department of Thoracic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China.

Background: Integrins play a crucial role in the regulation process of cell proliferation, migration, differentiation, tumor invasion and metastasis. , and are three encoding genes of integrins family. Accumulative evidences have proved that abnormal expression of , and are a common phenomenon in different malignances. However, their expression patterns and prognostic roles for patients with non-small cell lung cancer (NSCLC) have not been completely illustrated.

Methods: We investigated the expression patterns and prognostic values of , and in patients with NSCLC through using a series of databases and various datasets, including ONCOMINE, GEPIA, HPA, TCGA and GEO datasets.

Results: We found that the expression levels of and were significantly upregulated in both LUAD and LUSC, while was obviously upregulated in LUSC. Additionally, higher expression level of revealed a worse OS in LUAD.

Conclusion: Our findings suggested that and might have the potential ability to act as diagnostic biomarkers for both LUAD and LUSC, while might serve as diagnostic biomarker for LUSC. Furthermore, could serve as a potential prognostic biomarker for LUAD.
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http://dx.doi.org/10.7717/peerj.8299DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6927340PMC
December 2019

Characterization of the Interaction of Neuropathy Target Esterase with the Endoplasmic Reticulum and Lipid Droplets.

Biomolecules 2019 12 9;9(12). Epub 2019 Dec 9.

Chongqing Key Laboratory of Big Data for Bio-Intelligence, School of Bio-information, Chongqing University of Posts and Telecommunications, Chongqing 400065, China.

Neuropathy target esterase (NTE) is an endoplasmic reticulum (ER)-localized phospholipase that deacylates phosphatidylcholine (PC) and lysophosphatidylcholine (LPC). Loss-of-function mutations in the human NTE gene have been associated with a spectrum of neurodegenerative disorders such as hereditary spastic paraplegia, ataxia and chorioretinal dystrophy. Despite this, little is known about structure-function relationships between NTE protein domains, enzymatic activity and the interaction with cellular organelles. In the current study we show that the C-terminal region of NTE forms a catalytically active domain that exhibits high affinity for lipid droplets (LDs), cellular storage organelles for triacylglycerol (TAG), which have been recently implicated in the progression of neurodegenerative diseases. Ectopic expression of the C domain in cultured cells decreases cellular PC, elevates TAG and induces LD clustering. LD interactions of NTE are inhibited by default by a non-enzymatic regulatory (R) region with three putative nucleotide monophosphate binding sites. Together with a N-terminal TMD the R region promotes proper distribution of the catalytic C-terminal region to the ER network. Taken together, our data indicate that NTE may exhibit dynamic interactions with the ER and LDs depending on the interplay of its functional regions. Mutations that disrupt this interplay may contribute to NTE-associated disorders by affecting NTE positioning.
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http://dx.doi.org/10.3390/biom9120848DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6995513PMC
December 2019

Two Preputial Gland-Secreted Pheromones Evoke Sexually Dimorphic Neural Pathways in the Mouse Vomeronasal System.

Front Cell Neurosci 2019 2;13:455. Epub 2019 Oct 2.

College of Life Sciences, Zhejiang University, Hangzhou, China.

Hexadecanol (16OH) and hexadecyl acetate (16Ac) are two pheromones secreted in a large quantity by mouse preputial glands and act on male and female mice differentially. Yet the underlying molecular and cellular mechanisms remain to be elucidated. In this study, we examined the activation of vomeronasal sensory neurons (VSNs) by these two pheromones and mapped the downstream neural circuits that process and relay their chemosignals. Using the calcium imaging method and immunohistochemistry, we found that a small number of VSNs were activated by 16OH, 16AC, or both in the male and female mice, most of which were located apically in the vomeronasal epithelium, and their numbers did not increase when the concentrations of 16OH and 16Ac were raised by 10,000-fold except that of female VSNs in response to 16OH. In the accessory olfactory bulb (AOB), the two pheromones evoked more c-Fos+ neurons in the anterior AOB (aAOB) than in the posterior AOB (pAOB); and the increases in the number of c-Fos+ neurons in both aAOB and pAOB were dose-dependent; and between sexes, the female AOB responded more strongly to 16OH than to 16Ac whereas the male AOB had the opposite response pattern. This sexual dimorphism was largely retained in the downstream brain regions, including the bed nucleus of the stria terminalis (BNST), the medial amygdaloid nucleus (MeA), the posteromedial cortical amygdaloid nucleus (PMCo), the medial preoptic area (MPA), and the ventromedial hypothalamic nucleus (VmH). Taken together, out data indicate that there is one V1r receptor each for 16OH, 16Ac, or both, and that activation of these receptors evokes sexually dimorphic neural circuits, directing different behavioral outputs and possibly modulating other pheromone-induced responses.
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http://dx.doi.org/10.3389/fncel.2019.00455DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6783556PMC
October 2019

High-Density Shadows in a Patient With Cryptogenic Multifocal Ulcerous Stenosing Enteritis After Capsule Endoscopy.

Clin Gastroenterol Hepatol 2020 10 8;18(11):e133-e134. Epub 2019 Aug 8.

Department of General Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

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http://dx.doi.org/10.1016/j.cgh.2019.07.062DOI Listing
October 2020

High iodine induces DNA damage in autoimmune thyroiditis partially by inhibiting the DNA repair protein MTH1.

Cell Immunol 2019 Oct 2;344:103948. Epub 2019 Jul 2.

Thyroid Disease Diagnosis and Treatment Center, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China.

This study aims to investigate the level of DNA damage in high iodine (HI)-induced autoimmune thyroiditis (AIT), and to explore the role of DNA repair protein MutT homolog-1 (MTH1) in this process. The levels of pro-inflammatory cytokines tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-8 were measured using qRT-PCR and ELISA. The apoptosis was evaluated using TUNEL staining. The pathological changes of thyroid tissues were evaluated using hematoxylin and eosin (HE) staining. The DNA damage was assessed by determining the expression of 8-hydroxy-2'deoxyguanosine (8-OHdG; an indicator of oxidative DNA damage) and performing the Comet assay. Our results showed that both the HI-treated NOD.H-2 mice (experimental AIT mice) and the HI-treated mouse thyroid follicular epithelial cells showed enhanced inflammation, apoptosis, and DNA damage level, accompanied by decreased MTH1 expression. Importantly, overexpression of MTH1 effectively abrogated the HI-induced enhancement of inflammation, apoptosis, and DNA damage in mouse thyroid follicular epithelial cells. In conclusion, HI treatment induces DNA damage in AIT, at least in part, by inhibiting the DNA repair protein MTH1.
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http://dx.doi.org/10.1016/j.cellimm.2019.103948DOI Listing
October 2019

Calcifying fibrous tumor of the clivus presenting in an adult.

Radiol Case Rep 2019 Jun 10;14(6):771-774. Epub 2019 Apr 10.

Department of Radiology, Taihe hospital, affiliated to Hubei University of Medicine, Shiyan, Hubei, China.

Calcifying fibrous tumor is a benign fibrous tumor. It rarely occurs in the clivus. The present study describes a case of a 56-year-old female, who was admitted to Taihe Hospital with dizziness not accompanied with headache for 2 months. Brain computed tomography examination revealed a well-defined, partially calcified lytic-expansile lesion in the clivus, which corresponded to an enhancing mass on contrast-enhanced magnetic resonance imaging. The patient underwent endoscopic resection. Subsequent pathologic examination of the resected tissue confirmed that the tumor was calcifying fibrous tumor. The patient was followed up for 3 months after operation without recurrence or metastasis.
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http://dx.doi.org/10.1016/j.radcr.2019.03.028DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6460248PMC
June 2019

Initial treatment combined with Prunella vulgaris reduced prednisolone consumption for patients with subacute thyroiditis.

Ann Transl Med 2019 Feb;7(3):45

Thyroid Disease Diagnosis and Treatment Center, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China.

Background: Subacute thyroiditis (SAT) is a self-limited disease commonly treated with prednisolone (PSL). The present study aimed to assess the effectiveness and safety of the new treatment protocol which consisted of PSL and Prunella vulgaris (PV) to reduce the dosage and incidence of side effects from PSL.

Methods: Data regarding the patients with SAT treated at the First Affiliated Hospital, School of Medicine, Zhejiang University between September 2013 and May 2016 were retrospectively analyzed. Patients treated with PSL only or a combination of PSL and PV were divided into two groups.

Results: In total, 87 patients were included. Patients treated with PSL and PV showed a similar remission rate to the patients who received only PSL. The recurrence rate (4.4% . 9.5%) and incidence of transient hypothyroidism (2.2% . 4.8%) were similar between the two groups (P=0.350 and P=0.517, respectively). Only two cases of a mild skin rash were recorded, and these were related to the use of PV.

Conclusions: The combination of low-dose PSL as a treatment choice for SAT was both effective and safe.
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http://dx.doi.org/10.21037/atm.2019.01.07DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6389583PMC
February 2019

Distribution of ALK Fusion Variants and Correlation with Clinical Outcomes in Chinese Patients with Non-Small Cell Lung Cancer Treated with Crizotinib.

Target Oncol 2019 04;14(2):159-168

Department of Thoracic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, 9 Dongdan Santiao, Dongcheng District, Beijing, 100730, China.

Background: ALK-rearranged non-small cell lung cancer (NSCLC) represents a molecular subgroup with high sensitivity to ALK inhibitors. Crizotinib, a US Food and Drug Administration (FDA)-approved tyrosine kinase inhibitor for treating ALK-rearranged NSCLC, has shown remarkable response in ALK-positive NSCLC. However, heterogeneity of clinical responses exists among different ALK fusion partners. Several small studies have investigated the correlation between fusion partners and efficacy, but not yielded consistent results.

Objective: We investigated the prevalence of ALK rearrangements in a Chinese NSCLC population, and correlated clinical outcomes of crizotinib with different ALK partners/variants.

Patients And Methods: We retrospectively reviewed genomic profiling and clinical data of 110 ALK-rearranged NSCLC patients from five centers. The clinical response to crizotinib and survival data in ALK-positive patients was retrospectively analyzed.

Results: A total of 134 ALK rearrangements with 39 partners were identified in 110 patients (5.6%) among a cohort of 1971 NSCLC patients. The most frequently occurring ALK fusion partner was EML4, which was identified in 71.6% (96/134) of all of the rearrangements in 87.3% (96/110) patients, and with variant 3 (41/96, 42.7%) as the main variant type. No statistically significant differences in terms of progression-free survival (PFS) and overall survival (OS) were found between EML4-ALK and non-EML4-ALK NSCLC patients in our cohort (PFS, p = 0.207; OS, p = 0.678). Outcomes did not differ significantly between patients above and below 40 years of age (PFS, p = 0.427; OS, p = 0.686), nor between patients treated with crizotinib in different lines of therapy (PFS, p = 0.171; OS, p = 0.922). For EML4-ALK-positive NSCLC (n = 96), patients harboring variant 3 or variant 5 displayed significantly lower PFS and OS than those with other variants (PFS, 8.6 vs. 11.3 months, p = 0.046; OS, 31.0 vs. 37.6 months, p = 0.026). In addition, patients with a single EML4-ALK rearrangement event displayed favorable PFS (10.0 vs. 7.2 months, p = 0.040) and OS (36.0 vs. 20.0 months, p = 0.029) compared to those harboring multiple ALK rearrangements.

Conclusions: This study illustrates the patterns of ALK fusion variants present in Chinese NSCLC patients and might help explain heterogeneous clinical outcomes to crizotinib treatment according to different ALK fusion variants.
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http://dx.doi.org/10.1007/s11523-019-00631-xDOI Listing
April 2019

Evaluation of clinical risk factors for predicting insidious right central and posterior right recurrent laryngeal nerve lymph node metastasis in papillary thyroid microcarcinoma patients (cN0): experience of a single center.

Ann Transl Med 2019 Jan;7(1)

Thyroid Disease Diagnosis and Treatment Center, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China.

Background: Papillary thyroid microcarcinoma (PTMC), one specific subtype of papillary thyroid carcinoma (PTC) which measures less than 10 mm in maximum dimension, presents with a high risk of insidious lymph node metastasis escaping from preoperative examinations (cN0). Given the complications of lymph node dissection (LND) and metastasis risk, proper stratification of PTMC for performing prophylactic LND bears great importance.

Methods: From June 2015 to December 2017, 338 PTMC patients undergoing thyroidectomy were included in the present study. Potential risk factors, including age, gender, maximal tumor size, etc. were collected and analyzed for association with thyroid lymph node metastasis.

Results: Among the 338 patients, 87 patients (25.7%) presented with right central lymph node metastasis (CLNM) and 28 patients (8.3%) had posterior right recurrent laryngeal nerve lymph node metastasis (PRRLN-LNM). The maximal tumor was prone to occur at the middle part of the lower pole (35.3%) in patients with right CLNM, while the proportion of tumors located in the middle part of the upper pole (17.2%) was highest in PRRLN-LNM patients. Ages younger than 45 years old, male gender, and a tumor size of more than 0.5 cm were correlated independently with right CLNM and PRRLN-LNM. Presence of capsular invasion also had a significant association with the occurrence of PRRLN-LNM.

Conclusions: Ages younger than 45, male gender, and a maximal tumor size larger than 0.5 cm, in addition to capsular invasion, were independent risk factors for stratification of PTMC patients. PTMC patients with these clinical characteristics were suggested to receive prophylactic LND in their initial thyroid surgeries.
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http://dx.doi.org/10.21037/atm.2018.12.43DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351383PMC
January 2019

An endoscopic ultrasonography-guided interstitial brachytherapy based special treatment-planning system for unresectable pancreatic cancer.

Oncotarget 2017 Oct 27;8(45):79099-79110. Epub 2017 Feb 27.

Department of Gastroenterology, the 307 Hospital of Academy of Military Medical Science, Beijing, China.

EUS-guided interstitial brachytherapy is promising in the treatment of unresectable malignant carcinoma adjacent to the digestive tract. The feasible treatment plan is not established. Thus, our study aimed to develop a novel treatment plan and evaluate the feasibility in patients with unresectable pancreatic cancer. A total of 42 patients with unresectable pancreatic cancer (stage III: = 18; stage IV: = 24) were retrospectively included. A special treatment-planning system (TPS) for EUS was designed and evaluated by comparing with the traditional TPS. The patients underwent EUS-guided interstitial brachytherapy based on the new software. In the test model, there was no obvious difference of irradiation doses calculated by the two softwares (EUS TPS vs. traditional TPS) ( > 0.05). Under the support of EUS TPS, a novel treatment plan for EUS-guided interstitial brachytherapy was successfully established, which contained seven principles. All patients tolerated the treatment well without any serious complications. In 15 patients (stage III) whose minimal peripheral dose was larger than 90 Gy, partial remission rate was 80% (12/15). Twelve patients (12/18) in stage III were alive for over 12 months with a median peripheral dose of 107.5 Gy. The expected median survival time of the 42 patients was 9.0 months (95%CI 7.6-10.4 months). The results demonstrated that the new EUS TPS will play an important role in EUS-guided interstitial brachytherapy in patients with unresectable pancreatic malignant cancer.
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http://dx.doi.org/10.18632/oncotarget.15763DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5668023PMC
October 2017

Dependent Relationship between Quantitative Lattice Contraction and Enhanced Oxygen Reduction Activity over Pt-Cu Alloy Catalysts.

ACS Appl Mater Interfaces 2017 Oct 9;9(41):35740-35748. Epub 2017 Oct 9.

State Key Laboratory of Chemical Resource Engineering, Beijing Key Laboratory of Electrochemical Process and Technology for Materials, Beijing University of Chemical Technology , Beijing 100029, China.

Lattice contraction has been regarded as an important factor influencing oxygen reduction reaction (ORR) activity of Pt-based alloys. However, the relationship between quantitative lattice contraction and ORR activity has rarely been reported. Herein, using Pt-Cu alloy nanoparticles (NPs) with similar particle sizes but different compositions as examples, we investigated the relationship between quantitative lattice contraction and ORR activity by defining the shrinking percentage of Pt-Pt bond distance as lattice contraction percentage. The results show that the ORR activities of Pt-Cu alloy NPs exhibit a well-defined volcano-type dependent relationship toward the lattice contraction percentage. The dependent correlation can be explained by the Sabatier principle. This study not only proposes a valid descriptor to bridge the activity and atomic composition but also provides a reference for understanding the composition-structure-activity relationship of Pt-based alloys.
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http://dx.doi.org/10.1021/acsami.7b08437DOI Listing
October 2017
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