Publications by authors named "Yijun Li"

126 Publications

Efficient Extraction and Determination of Carbamate Pesticides in Vegetables Based on a Covalent Organic Frameworks with Acylamide Sites.

J Chromatogr A 2022 Jan 2;1664:462799. Epub 2022 Jan 2.

College of Chemistry, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Nankai University, Tianjin 300071, China; Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

It is an important challenge to effectively extract and determine pesticides in complex samples. Covalent organic frameworks (COFs) are burgeoning porous crystalline organic materials with good environmental resistance, thus demonstrating great potential as adsorbents in contaminants detection. In this work, we design and synthesize a novel COF-TpDB via 1,3,5-triformylphloroglucinol (Tp) and 4,4'-diaminobenzoylanilide (DB) as well as its packed cartridge for solid phase extraction (SPE) of carbamate pesticides. Simulation calculations showed H-bonding facilitates the adsorption interactions between the carbamate pesticides and TpDB. A method was developed by coupling TpDB as SPE sorbents with high performance liquid chromatography-ultraviolet (HPLC-UV) detection to determine trace carbamate pesticides in vegetables. The established method showed a wide linear range of 0.1-200 ng mL and low limit of detections (0.005-0.05 ng mL) for four carbamate pesticides. The applicability of TpDB as adsorbent was investigated for determination of trace carbamate pesticides residue in vegetables with satisfactory recoveries of four carbamates in the range of 80.4-101.2%. The results demonstrated that the COF-TpDB offer great potential for efficient extraction of carbamate pesticides from complicate matrices.
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http://dx.doi.org/10.1016/j.chroma.2021.462799DOI Listing
January 2022

A Review on Mechanochemistry: Approaching Advanced Energy Materials with Greener Force.

Adv Mater 2022 Jan 11:e2108327. Epub 2022 Jan 11.

State Key Laboratory of Polymer Materials Engineering, Polymer Research Institute of Sichuan University, Chengdu, 610065, China.

xxxx. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/adma.202108327DOI Listing
January 2022

Apolipoprotein B/A1 Ratio Is Associated with Severity of Coronary Artery Stenosis in CAD Patients but Not in Non-CAD Patients Undergoing Percutaneous Coronary Intervention.

Dis Markers 2021 18;2021:8959019. Epub 2021 Dec 18.

Department of Cardiovascular Medicine, The First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, China.

Background And Aims: Lipid metabolism plays important roles in atherosclerosis. Several studies have found that lipoprotein is associated with coronary artery disease (CAD) and hyperlipidemia. Although the roles of the apolipoprotein B/A1 ratio (ApoB/A1) were originally thought to be atherosclerotic, few studies have focused on the specific relationship between ApoB/A1 and severity of coronary artery stenosis with or without the presence of CAD.

Methods: A total of 6956 consecutive patients aged 21-98 years with suspected CAD who had undergone coronary angiography were enrolled. The severity of coronary lesions was evaluated using the Gensini score (GS). The relationships between ApoB/A1 and severity of coronary artery stenosis were evaluated.

Results: A total of 1795 non-CAD patients and 5161 CAD patients were included in the observational analysis. Patients with CAD had higher ApoB/A1 than individuals without CAD (0.67 (0.53-0.82) vs. 0.61 (0.49-0.75), < 0.001). In CAD patients, the higher the ApoB/A1 was, the higher the proportion of patients with MI, triple-vessel lesions, and higher Gensini scores. ApoB/A1 was significantly positively correlated with HbA1c and Gensini scores in CAD patients but not in non-CAD patients (all < 0.001). Logistic analyses showed that ApoB/A1 could be a risk factor for multivessel disease (OR: 2.768, 95% CI: 1.868-4.103, < 0.001). ApoB/A1 was found to be significantly positively correlated with the Gensini score in CAD patients.

Conclusions: ApoB/A1 is highly associated with the presence and severity of coronary artery stenosis in patients with CAD but not in non-CAD patients.
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http://dx.doi.org/10.1155/2021/8959019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8710153PMC
December 2021

Hydrogen peroxide potentiates antimicrobial photodynamic therapy in eliminating Candida albicans and Streptococcus mutans dual-species biofilm from denture base.

Photodiagnosis Photodyn Ther 2021 Dec 16;37:102691. Epub 2021 Dec 16.

Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key lab of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, China. Electronic address:

Background: Candida albicans (C.albicans) is the primary pathogen of denture biofilm. Moreover, it could establish a cross-kingdom relationship with bacteria to enhance its virulence and resistance to antifungal drugs. This study aimed to investigate the efficacy of antimicrobial photodynamic therapy (aPDT) in combination with hydrogen peroxide (HO) against C.albicans and Streptococcus mutans (S.mutans) dual-species biofilm formed on polymethyl methacrylate (PMMA) disk, and explore its involved mechanisms.

Methods: C.albicans and S.mutans were grown on PMMA disk for 48 h to form biofilm and received different treatments. The treatments included:1) phosphate-buffered saline (PBS) group,2) 100 mM HO group,3) aPDT group,4) aPDT+ HO and 5) HO+aPDT group. Colony forming units (CFU), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and scanning electron microscope (SEM) were used to evaluate the antimicrobial effects. Extracellular polysaccharide substance (EPS) production and observation, cell permeability of biofilm, and uptake of toluidine blue O (TBO) by biofilm were assessed to investigate the involved mechanism.

Results: There was no significant difference between PBS group and HO group in viable microorganisms and metabolic activity of biofilm. The treatment protocols containing aPDT group reduced microorganism numbers and metabolic activity when compared to PBS group or HO group (P<0.05). HO+aPDT treatment showed the highest antimicrobial efficacy in comparison with other treatments (P<0.05). Pretreatment with HO could decrease EPS production and enhance cell permeability, leading to increased TBO uptake in biofilm.

Conclusion: Pretreatment with HO improved aPDT efficiency in eliminating dual-species biofilm from PMMA disk by reducing EPS amount, enhancing cell permeability, and increasing TBO uptake.
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http://dx.doi.org/10.1016/j.pdpdt.2021.102691DOI Listing
December 2021

Bioinformatics analysis for the role of CALR in human cancers.

PLoS One 2021 15;16(12):e0261254. Epub 2021 Dec 15.

Departments of Breast Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, People's Republic of China.

Cancer is one of the most important public health problems in the world. The curative effect of traditional surgery, radiotherapy and chemotherapy is limited and has inevitable side effects. As a potential target for tumor therapy, few studies have comprehensively analyzed the role of CALR in cancers. Therefore, by using GeneCards, UALCAN, GEPIA, Kaplan-Meier Plotter, COSMIC, Regulome Explorer, String, GeneMANIA and TIMER databases, we collected and analyzed relevant data to conduct in-depth bioinformatics research on the CALR expression in Pan-cancer to assess the possibility of CALR as a potential therapeutic target and survival biomarker. We studied the CALR expression in normal human tissues and various tumors of different stages, and found that CALR expression was associated with relapse free survival (RFS). We verified the expression of CALR in breast cancer cell lines by vitro experiments. Mutations of CALR were widely present in tumors. CALR interacted with different genes and various proteins. In tumors, a variety of immune cells are closely related to CALR. In conclusion, CALR can be used as a biomarker for predicting prognosis and a potential target for tumor molecular and immunotherapy.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0261254PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8673678PMC
December 2021

Research on the cutoff tumor size of omitting radiotherapy for BCSS after breast conserving surgery in women aged 65 years or oder with low-risk invasive breast carcinoma: Results based on the SEER database.

Breast 2021 Dec 22;60:287-294. Epub 2021 Nov 22.

Department of Breast Surgery, First Affiliated Hospital of Xi'an Jiaotong University, 277 Yanta Western Rd., Xi'an, 710061, Shaanxi Province, China. Electronic address:

Background: Radiotherapy after breast-conserving surgery (BCS) is not always necessary in older women staged T1N0M0 with low-risk invasive breast cancer, but few studies have concluded the detailed tumor size as a reference for avoiding radiotherapy. The study was conducted to explore and identify the optimal cutoff tumor size.

Methods: The study population was from the Surveillance, Epidemiology, and End Results (SEER) database in 2010-2016. Propensity score matching was used to balance the confounders between groups. Predictors associated with survival were analyzed by Kaplan-Meier, X-tile, Cox proportional hazards model and competing risk model.

Results: A total of 52049 women and 3846 deaths were included in the cohort with a median follow-up of 34 months. Based on the cutoff value determined by X-tile analysis, the study population were divided into small tumor group (≤14 mm in diameter) and large tumor group (>14 mm in diameter). Small tumors and radiotherapy were correlated with better breast cancer-specific survival (BCSS). In subgroup analysis, the absolute benefit of BCSS in 6 years attributed to radiotherapy was only 0.90% (RT vs. non- RT:98.77% vs. 97.87%) for patients with small tumors but up to 3.33% (RT vs. non- RT:97.10% vs. 93.77%) for those with large tumors.

Conclusion: Small tumors and adjuvant radiotherapy were associated with improved long-term prognosis, and 14 mm in diameter was the cutoff tumor size of omitting radiotherapy for patients aged 65 or older with T1N0M0 stage, ER+ and HER2-breast carcinoma after BCS.
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http://dx.doi.org/10.1016/j.breast.2021.11.015DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8714502PMC
December 2021

Femtosecond all-polarization-maintaining Nd fiber laser at 920 nm mode locked by a biased NALM.

Opt Express 2021 Nov;29(23):38199-38205

We demonstrate a femtosecond all-polarization-maintaining Nd fiber laser working at 920 nm mode locked by a biased non-linear loop mirror. The broadest spectral width of the pulse is 25.2 nm and the output power is 8 mW with 320 mW pump power. The measured pulse width is 109 fs with extra-cavity compression. The laser configuration of all-polarization-maintaining fiber can directly enhance the environmental stability of generated pulses. The seed pulses of the oscillator were amplified over 400 mW, which served as the light source for a two-photon microscope. To the best of our knowledge, this is the first demonstration of a 920 nm femtosecond Nd polarization-maintaining fiber laser based on a non-linear loop mirror.
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http://dx.doi.org/10.1364/OE.434767DOI Listing
November 2021

A convenient polyculture system that controls a shrimp viral disease with a high transmission rate.

Commun Biol 2021 11 11;4(1):1276. Epub 2021 Nov 11.

State Key Laboratory for Biocontrol, School of Marine Sciences, Sun Yat-Sen University, Zhuhai, 519000, China.

Developing ecological approaches for disease control is critical for future sustainable aquaculture development. White spot syndrome (WSS), caused by white spot syndrome virus (WSSV), is the most severe disease in cultured shrimp production. Culturing specific pathogen-free (SPF) broodstock is an effective and widely used strategy for controlling WSS. However, most small-scale farmers, who predominate shrimp aquaculture in developing countries, cannot cultivate SPF shrimp, as they do not have the required infrastructure and skills. Thus, these producers are more vulnerable to WSS outbreaks than industrial farms. Here we developed a shrimp polyculture system that prevents WSS outbreaks by introducing specific fish species. The system is easy to implement and requires no special biosecurity measures. The promotion of this system in China demonstrated that it allowed small-scale farmers to improve their livelihood through shrimp cultivation by controlling WSS outbreaks and increasing the production of ponds.
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http://dx.doi.org/10.1038/s42003-021-02800-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8585955PMC
November 2021

Nutrient Alteration Drives the Impacts of Seawater Acidification on the Bloom-Forming Dinoflagellate .

Front Plant Sci 2021 21;12:739159. Epub 2021 Oct 21.

College of Marine Life Science, Ocean University of China, Qingdao, China.

Seawater acidification and nutrient alteration are two dominant environmental factors in coastal environments that influence the dynamics and succession of marine microalgae. However, the impacts of their combination have seldom been recorded. A simulated experimental system was set up to mimic the effects of elevated acidification on a bloom-forming dinoflagellate, , exposed to different nutrient conditions, and the possible mechanism was discussed. The results showed that acidification at different pH levels of 7.6 or 7.4 significantly influenced microalgal growth (<0.05) compared with the control at pH 8.0. Mitochondria, the key sites of aerobic respiration and energy production, were impaired in a pH-dependent manner, and a simultaneous alteration of reactive oxygen species (ROS) production occurred. Cytochrome c oxidase (COX) and citrate synthase (CS), two mitochondrial metabolism-related enzymes, were actively induced with acidification exposure, suggesting the involvement of the mitochondrial pathway in coping with acidification. Moreover, different nutrient statuses indicated by various N:P ratios of 7:1 (N limitation) and 52:1 (P limitation) dramatically altered the impacts of acidification compared with those exposed to an N:P ratio of 17:1 (control), microalgal growth at pH 7.4 was obviously accelerated with the elevation of the nutrient ratio compared to that at pH 8.1 (<0.05), and nutrient limitations seemed beneficial for growth in acidifying conditions. The production of alkaline phosphatase (AP) and acid phosphatase (AcP), an effective index indicating the microalgal growth status, significantly increased at the same time (<0.05), which further supported this speculation. However, nitrate reductase (NR) was slightly inhibited. Hemolytic toxin production showed an obvious increase as the N:P ratio increased when exposed to acidification. Taken together, mitochondrial metabolism was suspected to be involved in the process of coping with acidification, and nutrient alterations, especially P limitation, could effectively alleviate the negative impacts induced by acidification. The obtained results might be a possible explanation for the competitive fitness of during bloom development.
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http://dx.doi.org/10.3389/fpls.2021.739159DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8572056PMC
October 2021

Clinical Value and Potential Mechanisms of Oxysterol-Binding Protein Like 3 (OSBPL3) in Human Tumors.

Front Mol Biosci 2021 19;8:739978. Epub 2021 Oct 19.

Department of Breast Surgery, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Cancer remains one of the top culprits causing disease-related deaths. A lack of effective multi-cancer therapeutic targets has limited the prolongation of cancer patients' survival. Therefore, it is important to explore novel oncogenic genes or versatile targets and perform a comprehensive analysis to assess their roles in the process of tumorigenesis. OSBPL3 protein is an intracellular lipid receptor of the oxysterol-binding protein superfamily, which participates in some pathological and physiological processes in tumor progression. However, its clinical roles and potential mechanisms in cancers remain unknown. Thus, we aimed to systematic explore the potential oncogenic roles of OSBPL3 across thirty-three tumors using multiple web-based and publicly available tools, including the Cancer Genome Atlas, Gene Expression Omnibus, Genotype-Tissue Expression, cBioPortal, and Human Protein Atlas database. OSBPL3 is highly expressed in major subtypes of cancers, distinctly associated with the prognosis of tumor patients. We observed X676_splice/V676G alteration in the oxysterol domain and frequent mutations of OSBPL3 involve cell survival in skin cutaneous melanoma. We also first presented that the expression of OSBPL3 was associated with tumor mutational burden (TMB) in nine cancer types. Additionally, OSBPL3 shows an enhanced phosphorylation level at S426, S251, and S273 loci within the pleckstrin homology domain in multiple tumors, such as breast cancer or lung adenocarcinoma. And OSBPL3 expression was associated with active immune cells (CD8 T cells) and cancer-associated fibroblasts in breast cancer, colon adenocarcinoma, and kidney renal clear cell carcinoma and immune checkpoint genes in more than 30 tumors, but weakly associated with immune suppressive cells (myeloid-derived suppressor cells, T regulatory cells). Moreover, protein processing and mRNA metabolic signaling pathways were involved in the functional mechanisms of OSBPL3. Our study first demonstrated that a novel agent OSBPL3 plays an important role in tumorigenesis from the perspective of publicly available databases and clinical tumor samples in various cancers, which comprehensively provide insights into its biological functions and may be helpful for further investigation.
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http://dx.doi.org/10.3389/fmolb.2021.739978DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8560696PMC
October 2021

Chronic apical periodontitis exacerbates atherosclerosis in apolipoprotein E-deficient mice and leads to changes in the diversity of gut microbiota.

Int Endod J 2022 Feb 16;55(2):152-163. Epub 2021 Nov 16.

Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key lab of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, China.

Aim: To investigate the impact of chronic apical periodontitis (CAP) on atherosclerosis and gut microbiota by establishing a Porphyromonas gingivalis (P. gingivalis)-induced CAP in an apolipoprotein E-deficient (apoE ) mice model.

Methodology: Twenty-eight male apoE mice were divided into two groups with 14 in each: CAP group and control group. In the CAP group, sterile cotton wool containing 10 colony-forming units of P. gingivalis was placed into the pulp chamber after pulp exposure followed by coronal resin filling in bilateral maxillary first and second molars. The mice were fed with a chow diet to induce atherosclerosis. Animals were euthanized 16 weeks after the operation, and the periapical lesions of bilateral maxillary first and second molars were assessed by micro-CT. After collection of aortic arches, atherosclerotic lesions were measured by Oil Red O staining. Serum levels of high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), total cholesterol (TC), and triglycerides (TG) were measured. Stools were collected to detect alterations in gut microbiota by 16S rRNA gene sequencing. Independent samples t-test was used to calculate the difference between the two groups.

Results: CAP was observed in 98.2% of molars. A significant increase in atherosclerotic plaque formation in the aortic arches was found in the CAP groups (CAP: 2.001% ± 0.27%, control: 0.927% ± 0.22%, p = .005). No significant difference was observed between sevum level of HDL-C (CAP: 2.295 ± 0.31 mmol/L, Control: 3.037 ± 0.55 mmol/L, p = .264) or LDL-C (CAP: 17.066 ± 3.95 mmol/L, Control: 10.948 ± 1.69 mmol/L, p = .177) in CAP group and Control group. There were no significant differences in TG (CAP: 1.076 ± 0.08 mmol/L, control: 1.034 ± 0.13 mmol/L, p = .794) or TC (CAP: 6.372 ± 0.98 mmol/L, control: 6.679 ± 0.75 mmol/L, p = .72) levels between the two groups (p > .05). The alpha diversity was elevated in the CAP group. In terms of beta diversity, the CAP and control groups were clearly distinguished by the microbial community.

Conclusion: In a mouse experimental model, pulp infection with P. gingivalis -induced CAP, thus aggravating the development of atherosclerosis. Meanwhile, CAP increased alpha diversity and altered the beta diversity of the gut microbiota.
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http://dx.doi.org/10.1111/iej.13655DOI Listing
February 2022

Multiplexed detection of heavy metal ions by single plasmonic nanosensors.

Biosens Bioelectron 2022 Jan 6;196:113688. Epub 2021 Oct 6.

Tianjin Key Laboratory of Wireless Mobile Communications and Power Transmission, College of Electronic and Communication Engineering, Tianjin Normal University, Tianjin, 300387, China; Center for Sensor Technology of Environment and Health, School of Environment, Tsinghua University, Beijing, 100084, China. Electronic address:

Detection of multiple analytes simultaneously in small liquid samples with high efficiency and precision is highly important to the fields like water quality monitoring. In this letter, we present a multiplexed nanosensors with position-encoded aptamer functionalized gold nanorods for heavy metal ions detection. The individual gold nanorods respond specifically to two different heavy metal ions (Pb and Hg) with a spectral shift in the scattering spectrum. We used a home-built spectral imaging dark-field microscope to measure the response of thousands of single plasmonic nanosensors with relatively high time resolution and precision. To explore the performance and limit of detection (LOD) of our nanosensor and setup, we recorded the concentration-dependent response of our position-encoded nanosensors with a series of mixture solutions that contain different concentrations of Hg and Pb ions. The LOD levels of our system are around 5 nM for Pb ions and 1 nM for Hg ions. Our method and results demostrate the nanomolar sensitivity and the potential to detect more different heavy metal ions.
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http://dx.doi.org/10.1016/j.bios.2021.113688DOI Listing
January 2022

Coaxial Electrospun PLLA Fibers Modified with Water-Soluble Materials for Oligodendrocyte Myelination.

Polymers (Basel) 2021 Oct 19;13(20). Epub 2021 Oct 19.

Department of Cell Biology, Second Military Medical University, Shanghai 200433, China.

Myelin sheaths are essential in maintaining the integrity of axons. Development of the platform for in vitro myelination would be especially useful for demyelinating disease modeling and drug screening. In this study, a fiber scaffold with a core-shell structure was prepared in one step by the coaxial electrospinning method. A high-molecular-weight polymer poly-L-lactic acid (PLLA) was used as the core, while the shell was a natural polymer material such as hyaluronic acid (HA), sodium alginate (SA), or chitosan (CS). The morphology, differential scanning calorimetry (DSC), Fourier transform infrared spectra (FTIR), contact angle, viability assay, and in vitro myelination by oligodendrocytes were characterized. The results showed that such fibers are bead-free and continuous, with an average size from 294 ± 53 to 390 ± 54 nm. The DSC and FTIR curves indicated no changes in the phase state of coaxial brackets. Hyaluronic acid/PLLA coaxial fibers had the minimum contact angle (53.1° ± 0.24°). Myelin sheaths were wrapped around a coaxial electrospun scaffold modified with water-soluble materials after a 14-day incubation. All results suggest that such a scaffold prepared by coaxial electrospinning potentially provides a novel platform for oligodendrocyte myelination.
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http://dx.doi.org/10.3390/polym13203595DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537353PMC
October 2021

Downregulation of the FBXO43 gene inhibits tumor growth in human breast cancer by limiting its interaction with PCNA.

J Transl Med 2021 10 13;19(1):425. Epub 2021 Oct 13.

Department of Surgical Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, 277 West Yanta Road, Xi'an, 710061, Shaanxi, China.

Background: The function and regulatory mechanism of FBXO43 in breast cancer (BC) are still unclear. Here, we intended to determine the role and mechanism of FBXO43 in BC.

Methods: FBXO43 expression in BC was evaluated by analysis of The Cancer Genome Atlas (TCGA). RT-qPCR and western blotting were utilized to detect FBXO43 expression in BC cell lines. Lentivirus was applied to downregulate FBXO43 in human BC cells. Proliferation assays were performed to evaluate the proliferative ability of BC cells. The apoptosis and cell cycle analysis of BC cells were analyzed by flow cytometry. Cell migration and invasion were investigated via Transwell assays. The function of FBXO43 in vivo was evaluated by constructing a xenograft mouse model. The proteins that might interact with FBXO43 in BC were identified by mass spectrometry, bioinformatics analysis, and co-immunoprecipitation (Co-IP) assays. Finally, rescue experiments were conducted to validate the recovery effects of the proteins interacting with FBXO43.

Results: FBXO43 was highly expressed in BC and was significantly downregulated after FBXO43 knockdown. The proliferation, migration, and invasion of BC cells were inhibited, and cell apoptosis was induced by FBXO43 knockdown. In addition, an in vivo experiment indicated that FBXO43 knockdown could inhibit the cell growth of BC. The results of the Co-IP assay showed that FBXO43 interacted with PCNA. Further rescue experiments confirmed that overexpression of PCNA significantly reversed the effects of FBXO43 knockdown on BC cells.

Conclusion: Downregulation of FBXO43 inhibits the tumor growth of BC by limiting its interaction with PCNA. FBXO43 might be a new potential oncogene and a therapeutic target for BC.
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http://dx.doi.org/10.1186/s12967-021-03100-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8513237PMC
October 2021

The Bloom-Forming Dinoflagellate Adopts Different Growth Modes When Exposed to Short or Long Period of Seawater Acidification.

Toxins (Basel) 2021 09 8;13(9). Epub 2021 Sep 8.

Department of Marine Ecology, College of Marine Life Science, Ocean University of China, Qingdao 266003, China.

Impacts of ocean acidification (OA) on noncalcifying organisms and the possibly responsible mechanism have aroused great research interests with the intensification of global warming. The present study focused on a noxious, noncalcifying, bloom-forming dinoflagellate, (), and its variation of growth patterns exposed to different periods of seawater acidification with stressing gradients was discussed. The dinoflagellates under short-time acidifying stress (2d) with different levels of CO presented significant growth inhibition ( < 0.05). The cell cycle was obviously inhibited at S phase, and the photosynthetic carbon fixation was also greatly suppressed ( < 0.05). Apoptosis was observed and the apoptotic rate increased with the increment of CO. Similar tendencies were observed in the key components of mitochondrial apoptotic pathway (the mitochondrial membrane potential (MMP), Caspase-3 and -9, and Bax/Bcl-2 ratio). However, under prolonged stressing time (8 d and 15 d), the growth of dinoflagellates was recovered or even stimulated, the photosynthetic carbon fixation was significantly increased ( < 0.05), the cell cycle of division presented little difference with those in the control, and no apoptosis was observed ( > 0.05). Besides, acidification adjusted by HCl addition and CO enrichment resulted in different growth performances, while the latter had a more negative impact. The results of present study indicated that (1) the short-time exposure to acidified seawater led to reduced growth performance via inducing apoptosis, blocking of cell cycle, and the alteration in photosynthetic carbon fixation. (2) had undergone adaptive changes under long-term exposure to CO induced seawater acidification. This further demonstrated that has strong adaptability in the face of seawater acidification, and this may be one of the reasons for the frequent outbreak of red tide. (3) Ions that dissociated by the dissolved CO, instead of H itself, were more important for the impacts induced by the acidification. This work thus provides a new perspective and a possible explanation for the dominance of during the occurrence of HABs.
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http://dx.doi.org/10.3390/toxins13090629DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8470136PMC
September 2021

Self-Poled Poly(vinylidene fluoride)/MXene Piezoelectric Energy Harvester with Boosted Power Generation Ability and the Roles of Crystalline Orientation and Polarized Interfaces.

ACS Appl Mater Interfaces 2021 Oct 21;13(39):46738-46748. Epub 2021 Sep 21.

State Key Laboratory of Polymer Materials Engineering, Polymer Research Institute of Sichuan University, Chengdu, Sichuan 610065, China.

Micropiezoelectric devices have become one of the most competitive candidates for use in self-powered flexible and portable electronic products because of their instant response and mechanic-electric conversion ability. However, achievement of high output performance of micropiezoelectric devices is still a significant and challenging task. In this study, a poly(vinylidene fluoride) (PVDF)/MXene piezoelectric microdevice was fabricated through a microinjection molding process. The synergistic effect of both an intense shear rate (>10 s) as well as numerous polar C-F functional groups in MXene flakes promoted the formation of β-form crystals of PVDF in which the crystallinity of β-form could reach as high as 59.9%. Moreover, the shear-induced shish-kebab crystal structure with a high orientation degree ( = ∼0.9) and the stacked MXene acted as the driving force for the dipoles to regularly arrange and produce a self-polarizing effect. Without further polarization, the fabricated piezoelectric microdevices exhibited an open-circuit voltage of 15.2 V and a short-circuit current of 497.3 nA, under optimal conditions (400 mm s and 1 wt % MXene). Impressively, such piezoelectric microdevices can be used for energy storage and for sensing body motion to monitor exercise, and this may have a positive impact on next-generation smart sports equipment.
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http://dx.doi.org/10.1021/acsami.1c14007DOI Listing
October 2021

[Preparation of molecularly imprinted polymers-functionalized silica nanoparticles for the separation and recognition of aristolochic acids].

Se Pu 2021 Oct;39(10):1137-1145

College of Chemistry, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Nankai University, Tianjin 300071, China.

Aristolochic acids (AAs), which is commonly found in and plants, has been widely used in several traditional medicine practices due to their anti-inflammatory, anti-malarial, and anti-hyperglycemic activities. Recently, researchers have found a “decisive link” between liver cancer and aristolochic acid after analyzing a large number of liver cancer samples around the world. Therefore, a highly sensitive and selective method is required for the analysis of AAs in traditional Chinese medicines (TCM). For the determination of AAs in TCM, pretreatment is indispensable because in actual TCM samples, AAs is present in trace amounts and the complex matrix exerts interference. In the past decades, molecularly imprinted polymers (MIPs) have attracted considerable attention as an alternative for the trace analysis in complicated matrices. In this study, MIP-coated SiO nanoparticles ([email protected] NPs) was prepared for the determination of aristolochic acid by surface molecular imprinting using aristolochic acid Ⅰ (AAI ) as the template molecule, 2-vinylpyridine (VPY) as the functional monomer, and ethyleneglycol dimethacrylate (EGDMA) as the cross-linking agent. Core-shell-structure [email protected] NPs were obtained by modifying vinyl groups on the surface of SiO NPs, coating MIPs films onto the silica surface via selective polymerization, and final extraction of template AAI and generation of the recognition site. To find a suitable functional monomer for the best imprinting effect, the interaction between the template and the functional monomers, including acrylic acid (AA), methyl acrylic acid (MAA), 2-vinyl pyridine (VPY), acrylamide (AM), and methylacrylamide (MAM) was investigated. Electrostatic interaction between AAI and VPY resulted in the maximum decrease in absorbance of AAI at 250 nm. Therefore, VPY was chosen for the preparation of MIP. The morphological and physical properties of the MIPs were characterized by transmission electron microscopy (TEM), Fourier-transform infrared (FT-IR) spectroscopy, thermogravimetric analysis, and N adsorption and desorption surface analysis. TEM images showed that SiO NPs were monodispersed with diameter of about 200 nm. The clear core-shell structure of [email protected] NPs was observed, and the thickness of MIPs coating was about 35 nm. The FT-IR spectra of SiO NPs, vinyl group modified SiO and [email protected] NPs revealed that the vinyl group and organic MIP layer were successfully modified at SiO sequentially. The results of thermogravimetric analysis were consistent with the FT-IR data for different SiO NPs. The nitrogen gas adsorption-desorption experiments showed that [email protected] NPs and non-imprinted polymer ([email protected] NPs) have the same pore volumes, while the surface area and pore size of MIPs were slightly larger than those of NIPs. Therefore, the difference in adsorption between [email protected] NPs and [email protected] NPs resulted from the imprinted sites on the MIP surface, rather than the difference in their surface areas. The adsorption properties of [email protected] NPs were demonstrated by kinetic, isothermal, and selective adsorption experiments. The results of these experiments displayed that [email protected] NPs reached adsorption equilibrium within a short period (120 s) and possessed a much higher rebinding ability than [email protected] NPs. To verify the selectivity of [email protected] NPs for AAI, three structural analogues (viz. tanshinone ⅡA, 2-methoxy-5-nitrophenol, and benzoic acid) were selected. The results showed that the binding capacity of [email protected] NPs was much higher than those of these analogues. [email protected] NPs have high adsorption capacity (5.74 mg/g), high imprinting factor (4.9), good selectivity coefficient (2.3-6.6) towards the structural analogues. [email protected] NPs was used as an adsorbent and combined with HPLC for the selective separation of AAI in TCM. The recoveries of Kebia trifoliate samples spiked with three levels of AAI (0.3, 0.5, and 1.0 μg/mL) ranged from 73% to 83%. The results suggested that the proposed [email protected] NPs could be used for selective enrichment of AAI from real complex TCM samples.
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http://dx.doi.org/10.3724/SP.J.1123.2021.06024DOI Listing
October 2021

Sequential Release of Paclitaxel and Imatinib from Core-Shell Microparticles Prepared by Coaxial Electrospray for Vaginal Therapy of Cervical Cancer.

Int J Mol Sci 2021 Aug 16;22(16). Epub 2021 Aug 16.

Department of Pharmaceutics, School of Pharmacy, Fudan University & Key Laboratory of Smart Drug Delivery (Fudan University), Shanghai 201203, China.

To optimize the anti-tumor efficacy of combination therapy with paclitaxel (PTX) and imatinib (IMN), we used coaxial electrospray to prepare sequential-release core-shell microparticles composed of a PTX-loaded sodium hyaluronate outer layer and an IMN-loaded PLGA core. The morphology, size distribution, drug loading, differential scanning calorimetry (DSC), Fourier transform infrared spectra (FTIR), in vitro release, PLGA degradation, cellular growth inhibition, in vivo vaginal retention, anti-tumor efficacy, and local irritation in a murine orthotopic cervicovaginal tumor model after vaginal administration were characterized. The results show that such core-shell microparticles were of spherical appearance, with an average size of 14.65 μm and a significant drug-loading ratio (2.36% for PTX, 19.5% for IMN, /), which might benefit cytotoxicity against cervical-cancer-related TC-1 cells. The DSC curves indicate changes in the phase state of PTX and IMN after encapsulation in microparticles. The FTIR spectra show that drug and excipients are compatible with each other. The release profiles show sequential characteristics in that PTX was almost completely released in 1 h and IMN was continuously released for 7 days. These core-shell microparticles showed synergistic inhibition in the growth of TC-1 cells. Such microparticles exhibited prolonged intravaginal residence, a >90% tumor inhibitory rate, and minimal mucosal irritation after intravaginal administration. All results suggest that such microparticles potentially provide a non-invasive local chemotherapeutic delivery system for the treatment of cervical cancer by the sequential release of PTX and IMN.
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http://dx.doi.org/10.3390/ijms22168760DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8395827PMC
August 2021

Naloxone Protects against Lipopolysaccharide-Induced Neuroinflammation and Microglial Activation via Inhibiting ATP-Sensitive Potassium Channel.

Comput Math Methods Med 2021 29;2021:7731528. Epub 2021 Jul 29.

Department of Neurosurgery, Haimen People's Hospital, Nantong 226100, China.

Aim: The aim of this study was to evaluate the anti-inflammatory effects and underlying mechanism of naloxone on lipopolysaccharide- (LPS-) induced neuronal inflammation and microglial activation.

Methods: LPS-treated microglial BV-2 cells and mice were used to investigate the anti-inflammatory effects of naloxone.

Results: The results showed that naloxone dose-dependently promoted cell proliferation in LPS-induced BV-2 cells, downregulated the expression of proinflammatory cytokines (TNF-, IL-1, and IL-6) and proinflammatory enzymes iNOS and COX-2 as well as the expression of free radical molecule NO, and reduced the expression of Iba-1-positive microglia in LPS-stimulated BV-2 cells and mouse brain. Moreover, naloxone improved LPS-induced behavior degeneration in mice. Mechanically, naloxone inhibited LPS-induced activation in the ATP-sensitive potassium (KATP) channel. However, the presence of glibenclamide (Glib), an antagonist of KATP channel, ameliorated the suppressive effects of naloxone on inflammation and microglial activation.

Conclusion: Naloxone prevented LPS-induced neuroinflammation and microglial activation partially through the KATP channel. These findings might highlight the potential of naloxone in neuroinflammation therapy.
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http://dx.doi.org/10.1155/2021/7731528DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8349287PMC
November 2021

An exploratory data quality analysis of time series physiologic signals using a large-scale intensive care unit database.

JAMIA Open 2021 Jul 2;4(3):ooab057. Epub 2021 Aug 2.

Department of Health Policy and Management, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA.

Physiological data, such as heart rate and blood pressure, are critical to clinical decision-making in the intensive care unit (ICU). Vital signs data, which are available from electronic health records, can be used to diagnose and predict important clinical outcomes; While there have been some reports on the data quality of nurse-verified vital sign data, little has been reported on the data quality of higher frequency time-series vital signs acquired in ICUs, that would enable such predictive modeling. In this study, we assessed the data quality issues, defined as the completeness, accuracy, and timeliness, of minute-by-minute time series vital signs data within the MIMIC-III data set, captured from 16009 patient-ICU stays and corresponding to 9410 unique adult patients. We measured data quality of four time-series vital signs data streams in the MIMIC-III data set: heart rate (HR), respiratory rate (RR), blood oxygen saturation (SpO2), and arterial blood pressure (ABP). Approximately, 30% of patient-ICU stays did not have at least 1 min of data during the time-frame of the ICU stay for HR, RR, and SpO2. The percentage of patient-ICU stays that did not have at least 1 min of ABP data was ∼56%. We observed ∼80% coverage of the total duration of the ICU stay for HR, RR, and SpO2. Finally, only 12.5%%, 9.9%, 7.5%, and 4.4% of ICU lengths of stay had ≥ 99% data available for HR, RR, SpO2, and ABP, respectively, that would meet the three data quality requirements we looked into in this study. Our findings on data completeness, accuracy, and timeliness have important implications for data scientists and informatics researchers who use time series vital signs data to develop predictive models of ICU outcomes.
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http://dx.doi.org/10.1093/jamiaopen/ooab057DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8327372PMC
July 2021

Hierarchically Architected Polyvinylidene Fluoride Piezoelectric Foam for Boosted Mechanical Energy Harvesting and Self-Powered Sensor.

ACS Appl Mater Interfaces 2021 Aug 28;13(31):37252-37261. Epub 2021 Jul 28.

State Key Laboratory of Polymer Materials Engineering, Polymer Research Institute of Sichuan University, Chengdu 610065, China.

With the rapid development of wearable electronics, piezoelectric materials have received great attention owing to their potential solution to the portable power source. To enhance the output capability and broaden the application, it is highly desired for the design of piezoelectric materials with a three-dimensional and porous structure to facilitate strain accumulation. Herein, enlightened by hierarchical structures in nature, a hierarchically nested network was constructed in polyvinylidene fluoride (PVDF) foam via solid-state shear milling and salt-leaching technology. The as-prepared foam exhibited two hierarchical levels of pores with diameters of 20∼50 μm and 0.3∼4 μm, by which the porosity and flexibility were significantly enhanced, while the highest piezoelectric output reached 11.84 V and 217.78 nA. As a proof-of-concept, the PVDF piezoelectric foam can also be used to monitor human movement toward the different magnitude of strain and frequency, and simultaneously collect energy in a multidimensional stress field for energy harvesting. This work provides a simple and convenient design idea for the preparation of energy harvesters, which have great application potential as a mechanical energy harvester or self-powered sensor in wearable electronic devices.
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http://dx.doi.org/10.1021/acsami.1c11158DOI Listing
August 2021

Profiles of immune infiltration in the tumor microenvironment of hepatocellular carcinoma.

J Gastrointest Oncol 2021 Jun;12(3):1152-1163

Department of Surgical Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Background: Thus far, few studies have systematically analyzed the profiles of immune cells infiltrated in the tumor microenvironment (TME) of hepatocellular carcinoma (HCC). Therefore, the purpose of our study was to comprehensively analyze the 22 tumor-infiltrating immune cells (TIICs) and the immune subtypes of HCC, as well as the factors associated with the prognosis of HCC patients.

Methods: In this study, we evaluated the abundance of 22 tumor-infiltrating immunocytes of 371 HCC patients from The Cancer Genome Atlas (TCGA) database by using the CIBERSORT algorithm, and defined immune subtypes of HCC according to unsupervised cluster analysis. The immune score of HCC patients was calculated by the prognostic regression model, while the survival analysis was evaluated by the Kaplan-Meier method. In addition, the consistency index of TIICs and principal component analysis (PCA) of immunomodulator genes were estimated.

Results: The results of this study showed that three distinct immune subtypes of HCC were stratified, and the C1 subtype and C3 subtype were correlated with a good prognosis. The cellular composition of three immune subtypes was different. Moreover, immunomodulator gene and programmed cell death protein 1/programmed death-ligand 1 (PD-1/PD-L1) expression in the C1 subtype was significantly higher (P<0.05).

Conclusions: This suggested that the low immune score of HCC patients is associated with better clinical outcomes. In addition, the interaction network of cluster of differentiation CD8+ T cells was mainly concentrated in the C1 subtype. Taken together, this study showed that tumor-infiltrating immune cells can perhaps be an important determinant of clinical outcomes of patients with HCC and may provide biomarkers to reflect the immunotherapy response. Notably, the C1 subtype of HCC may be used as an important predictive factor for immunotherapy response.
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http://dx.doi.org/10.21037/jgo-21-291DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261307PMC
June 2021

A novel theranostic nanoplatform for imaging-guided chemo-photothermal therapy in oral squamous cell carcinoma.

J Mater Chem B 2021 08;9(30):6006-6016

State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China. and Med-X Center for Materials, Sichuan University, Chengdu, 610041, China.

Oral squamous cell carcinoma (OSCC) is highly malignant and invasive, and current treatments are limited due to serious side effects and unsatisfactory outcomes. Here, we reported the terbium ion-doped hydroxyapatite (HATb) nanoparticle as a luminescent probe to encapsulate both the near-infrared (NIR) photothermal agent polydopamine (PDA) and anticancer doxorubicin (DOX) for imaging-guided chemo-photothermal therapy. The morphology, crystal structure, fluorescence, and composition of HATb-PDA-DOX were characterized. HATb-PDA showed a high DOX loading capacity. A theranostic nanoplatform showed pH/NIR responsive release properties and better antitumor outcomes in OSCC cells than monomodal chemotherapy or photothermal therapy, while keeping side effects at a minimum. Also, the luminescence signal was confirmed to be tracked and the increase of the red/green (R/G) ratio caused by the DOX release could be used to monitor the DOX release content. Furthermore, HATb-PDA-DOX plus NIR treatment synergistically promoted in vitro cell death through the overproduction of reactive oxygen species (ROS), cell cycle arrest, and increased cell apoptosis. Overall, this work presents an innovative strategy in designing a multifunctional nano-system for imaging-guided cancer treatment.
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http://dx.doi.org/10.1039/d1tb01136gDOI Listing
August 2021

Topological Organization Alterations of Whole-Brain Functional Networks in Patients with Childhood Absence Epilepsy: Associations with Treatment Effects.

Dis Markers 2021 26;2021:2727596. Epub 2021 Jun 26.

Department of Cardiology, The Second Affiliated Hospital of Shandong First Medical University, Taian, China.

Purpose: The purpose of the current study is to detect changes of topological organization of whole-brain functional networks and their relationship with the clinical treatment effects of antiepileptic drugs (AEDs) for patients with childhood absence epilepsy (CAE) using resting-state functional MRI (RS-fMRI). . RS-fMRI data from 30 CAE patients were collected and compared with findings from 30 age- and gender-matched healthy controls (HCs). The patients were treated with first-line AEDs for 46.03 months before undergoing a second RS-fMRI scan.

Results: CAE children at baseline showed a reduced clustering coefficient (Cp) and local efficiency (El) than the HC group, implying the reduction of functional segregation. CAE children at baseline also showed smaller characteristic path length (Lp) and higher global efficiency (Eg) compared with the HC group, implying the impairment of functional segregation. However, those metrics showed no significant differences between CAE children at follow-up and the HC group which indicated a clear renormalization of topological organization after AED treatments. CAE at follow-up also showed significantly decreased connectivity between several network regions, with which the thalamus is mainly involved. Furthermore, the reduced connectivity change between the left superior parietal gyrus and the left thalamus is positively correlated with the symptom improvements after AED treatment.

Conclusion: We highlighted the convergence and divergence of brain functional network dysfunctions in CAE patients and provided crucial insights into pathophysiological mechanisms and the AED effects.
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http://dx.doi.org/10.1155/2021/2727596DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8257349PMC
December 2021

A nomogram based on clinicopathological features and serological indicators predicting breast pathologic complete response of neoadjuvant chemotherapy in breast cancer.

Sci Rep 2021 05 31;11(1):11348. Epub 2021 May 31.

Department of Breast Surgery, First Affiliate Hospital, Xi'an Jiaotong University, 277 Yanta West Road, Xi'an, 710061, People's Republic of China.

A single tumor marker is not enough to predict the breast pathologic complete response (bpCR) after neoadjuvant chemotherapy (NAC) in breast cancer patients. We aimed to establish a nomogram based on multiple clinicopathological features and routine serological indicators to predict bpCR after NAC in breast cancer patients. Data on clinical factors and laboratory indices of 130 breast cancer patients who underwent NAC and surgery in First Affiliated Hospital of Xi'an Jiaotong University from July 2017 to July 2019 were collected. Multivariable logistic regression analysis identified 11 independent indicators: body mass index, carbohydrate antigen 125, total protein, blood urea nitrogen, cystatin C, serum potassium, serum phosphorus, platelet distribution width, activated partial thromboplastin time, thrombin time, and hepatitis B surface antibodies. The nomogram was established based on these indicators. The 1000 bootstrap resampling internal verification calibration curve and the GiViTI calibration belt showed that the model was well calibrated. The Brier score of 0.095 indicated that the nomogram had a high accuracy. The area under the curve (AUC) of receiver operating characteristic (ROC) curve was 0.941 (95% confidence interval: 0.900-0.982) showed good discrimination of the model. In conclusion, this nomogram showed high accuracy and specificity and did not increase the economic burden of patients, thereby having a high clinical application value.
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http://dx.doi.org/10.1038/s41598-021-91049-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167133PMC
May 2021

Nomogram Predicts the Role of Contralateral Prophylactic Mastectomy in Male Patients With Unilateral Breast Cancer Based on SEER Database: A Competing Risk Analysis.

Front Oncol 2021 29;11:587797. Epub 2021 Apr 29.

Department of Breast Surgery, First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, China.

Background: Contralateral prophylactic mastectomy (CPM) in female breast cancer (FBC) is supported by multiple clinical studies and consensus guidelines, but knowledge of preventive contralateral mastectomy in male breast cancer (MaBC) is very limited and its benefits are still controversial.

Methods: A retrospective cohort study was enrolled with 4,405 MaBC patients who underwent unilateral mastectomy (UM) or CPM from the Surveillance, Epidemiology, and End Results (SEER) database from 1998 to 2015. A nomogram was built based on the corresponding parameters by competing risks regression to predict the 3-year, 5-year, and 8-year probabilities of BCSD (breast cancer-specific death). C-index and calibration curves were chosen for validation. Net reclassification index (NRI) and integrated discrimination improvement (IDI) were used to estimate the nomogram's clinical utility.

Results: A total of 4,197 patients received UM and 208 patients received CPM, with 63-months median follow-up. In the competing risks regression, six variables (surgery, marital status, T-stage, N-stage, histology, tumor grade) were significantly associated with BCSD. Based on these independent prognosis factors, a nomogram model was constructed. The C-index 0.75 (95%CI: 0.73-0.77) in the training cohort and 0.73 (95%CI: 0.71-0.74) in the internal validation group suggested robustness of the model. In addition, the calibration curves exhibited favorably. The NRI values (training cohort: 0.54 for 3-year, 0.55 for 5-year, and 0.49 for 8-year BCSD prediction; validation cohort: 0.51 for 3-year, 0.45 for 5-year, and 0.33 for 8-year BCSD prediction) and IDI values (training cohort: 0.02 for 3-year, 0.03 for 5-year, and 0.04 for 8-year BCSD prediction; validation cohort: 0.02 for 3-year, 0.04 for 5-year, and 0.04 for 8-year BCSD prediction) indicated that the model performed better than the AJCC criteria-based tumor staging alone.

Conclusions: The administration of CPM was associated with the decrease in risk of BCSD in patients with MaBC. The nomogram could provide a precise and personalized prediction of the cumulative risk in patients with MaBC after CPM.
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http://dx.doi.org/10.3389/fonc.2021.587797DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8117922PMC
April 2021

Determination of the triple oxygen isotopic composition of tropospheric ozone in terminal positions using a multistep nitrite-coated filter-pack system.

Rapid Commun Mass Spectrom 2021 Aug;35(15):e9124

National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki, 305-8506, Japan.

Rationale: The triple oxygen isotopic composition (Δ O) of tropospheric ozone (O ) is a useful tracer for identifying the source and is essential for clarifying the atmospheric chemistry of oxidants. However, the single nitrite-coated filter method is inaccurate owing to the nitrate blank produced through the reaction of nitrite and oxygen compounds other than O .

Methods: A multistep nitrite-coated filter-pack system is newly adopted to transfer the O-atoms in terminal positions of O to nitrite on each filter to determine the Δ O of O in terminal positions (denoted as Δ O(O ) ). The NO produced by this reaction is chemically converted into N O, and continuous-flow isotope ratio mass spectrometry (CF-IRMS) is used to determine the oxygen isotopic compositions.

Results: The reciprocal of the NO quantities on the nitrite-coated filters in each sample showed a strong linear relationship with Δ O of NO . Using the linear relation, we corrected the changes in Δ O of NO on the filters. We verified the accuracy of the new method through the measurement of artificial O with known Δ O(O ) value that had been determined from the changes in Δ O of O . The Δ O(O ) of tropospheric O was in agreement with previous studies.

Conclusions: We accurately determined the δ O and Δ O values of tropospheric O by blank correction using our new method. Measurements of Δ O(O ) of the ambient troposphere showed 1.1 ± 0.7‰ diurnal variations between daytime (higher) and nighttime (lower) due likely to the formation of the temperature inversion layer at night.
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http://dx.doi.org/10.1002/rcm.9124DOI Listing
August 2021

A strategy of utilizing Cu-mediating interaction to prepare magnetic imprinted polymers for the selective detection of celastrol in traditional Chinese medicines.

Talanta 2021 Aug 24;231:122339. Epub 2021 Mar 24.

College of Chemistry, Tianjin Key Laboratory of Biosensing and Molecular Recognition, State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, 300071, China; Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116011, China.

In this work, a novel strategy to prepare molecularly imprinted polymers (MIPs) functionalized magnetic carbon nanotubes (MCNTs) via a facile sol-gel polymerization by adopting Cu-mediating interaction was presented for selective recognition of celastrol (Cel), in the traditional Chinese medicines (TCM). Firstly, template Cel, 3-aminopropyltriethoxysilane (APTES) as monomer and Cu (co-monomer) were mixed to form a self-assembled pre-complex, in which Cu could coordinate with Cel. Meanwhile, APTES plays a role of bridge between APTES and Cel. Secondly, carboxyl modified MCNTs as substrate was added into the pre-complex solution. After that, a multi-step sol-gel polymerization process was occurred in the presence of tetraethylorthosilicate as cross-linker and acetic acid as catalyst. Finally, MIPs layer was formed on the surface of the MCNTs ([email protected]) after the removal of template with methanol/acetic. The morphology and structure of [email protected] was investigated by various characterization techniques. The adsorption performance of [email protected] to Cel was illustrated by kinetic, isothermal and selective binding experiments. The results displayed that the [email protected] possessed fast kinetic equilibrium time (40 s), high adsorption capacity (13.35 μg mg), good imprinting factor of 3.41, and high magnetic responsivity (44.38 emu·g), which can be used as an ideal adsorbent for rapid isolation and enrichment of target analytes. A selective and sensitive method based on [email protected] coupling with HPLC was developed for Cel determination including a wide linear range (0.15-200 μg mL) with correlation coefficient of 0.9998, a low limit of detection (0.05 μg mL). Furthermore, the applicability of [email protected] was demonstrated to isolate and determine Cel in TCM samples with satisfactory recoveries ranged from 84.47% to 91.5% (RSD<5.35%). The results revealed that [email protected] offer great potential as an adsorbent for selective and efficient isolation of Cel from complex TCM samples.
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http://dx.doi.org/10.1016/j.talanta.2021.122339DOI Listing
August 2021

Hydrophilic molecularly imprinted polymers functionalized magnetic carbon nanotubes for selective extraction of cyclic adenosine monophosphate from winter jujube.

J Sep Sci 2021 May 31;44(10):2131-2142. Epub 2021 Mar 31.

College of Chemistry, Tianjin Key Laboratory of Biosensing and Molecular Recognition, State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, P. R. China.

In this work, a green strategy was developed to prepare molecularly imprinted polymers functionalized magnetic carbon nanotubes in aqueous phase under mild conditions for cyclic adenosine monophosphate. Thanks to water solubility of chitosan, a natural polysaccharide which is rich in amino and hydroxyl groups, provided the feasibility to synthesize the green molecularly imprinted polymers for water soluble template in aqueous media. Coupled with high-performance liquid chromatography, the method exhibited a short equilibrium time (6 min), high adsorption capacity (22.42 μg/mg), high magnetic susceptibility, and good selectivity to template molecule with the imprinting factor of 2.94. A good linearity in the range of 0.020-3.0 mg/mL for target was obtained with a correlation coefficient of 0.9998. The limit of detection (signal-to-noise ratio = 3) and limit of quantitation (signal-to-noise ratio = 10) of the magnetic solid phase extraction method for cyclic adenosine monophosphate were 5 and 15 ng/mg, respectively. And the practical application of chitosan-based molecularly imprinted polymers as adsorbent to isolate and determine cyclic adenosine monophosphate in real natural samples (winter jujube) was demonstrated.
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http://dx.doi.org/10.1002/jssc.202001095DOI Listing
May 2021

The Effectiveness of Preoperative Correction Techniques in Improving Nasal Deformity in Children With Unilateral Complete Cleft Lip and Palate.

J Craniofac Surg 2021 Mar-Apr 01;32(2):664-669

Oral Department, Children's Hospital, Chongqing Medical University, Chongqing, China.

Objective: Unilateral complete cleft lip and palate (UCCLP) is associated with apparent nasal deformities before the cheilorrhaphy. The aim of this study was to determine whether preoperative correction techniques are effective in the treatment of nasal deformities in infant with unilateral UCCLP used by the systematic review and meta-analysis.

Methods: We searched Medline, Cochrane Library, EMBASE, PubMed, and Chinese BioMedical Literature Database (CBM) until January 31, 2019, to identify studies that compared the effectiveness of preoperative correction techniques in the treatment of nasal deformities in infant with UCCLP. Two authors individually extracted the data and performed the quality assessments. The height of nasal columella, the width of the affected side nasal ala and the inclination of the nasal columella were evaluated.

Results: Seven articles were incorporated into the systematic review, and 5 (274 participants) in the meta-analysis according to the inclusion criteria. The preoperative correction could increase the height of nasal columella in children with UCCLP [SMD: 2.64 mm; 95% confidence intervals (CI); (1.35 mm, 3.94 mm); P < 0.0001]. Moreover, the preoperative correction resulted in reduced width of the affected side nasal ala [SMD: -5.14 mm; 95% CI; (-8.96 mm, -1.31 mm); P = 0.008]; However, the evidence was insufficient to determine a significant effect on the inclination of the nasal columella [SMD: -3.48 degrees; 95% CI; (-7.56 degrees, 0.59 degrees); P = 0.09].

Conclusions: Preoperative correction for children with UCCLP can increase the height of nasal columella, reduce the width of the affected side nasal ala, improve the nasal symmetry, and reduce nasal deformity, however, no significant effect could be observed for the inclination of the nasal columella.
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http://dx.doi.org/10.1097/SCS.0000000000007145DOI Listing
June 2021
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