Publications by authors named "Yihui Dong"

16 Publications

  • Page 1 of 1

NLRP3 promotes immune escape by regulating immune checkpoints: A pan-cancer analysis.

Int Immunopharmacol 2022 Jan 10;104:108512. Epub 2022 Jan 10.

Department of Hepatobiliary Pancreatic Surgery, the First Hospital of Ningbo City, Ningbo 315010, China. Electronic address:

NLRP3 plays a pathogenic role in tumorigenesis by regulating innate and acquired immunity, apoptosis, differentiation, and intestinal microbes in tumors. Our research aimed to investigate the role of NLRP3 in pan-cancers based on multi-omics data in the TCGA database. Most types of tumors showed increased expression of NLRP3. Among them, the overexpressed NLRP3 in liver hepatocellular carcinoma (LIHC) and ovarian cancer (OV) indicated worse overall survival (OS). Further analysis also confirmed overexpressed NLRP3 in colon cancer (COAD) indicated a high probability of microsatellite instability (MSI) and low tumor mutational burden (TMB), which indicated a better response to immune checkpoint inhibitors (ICIs). Interestingly, overexpression of NLRP3 was closely related to high infiltration of immune cells (T cells, B cells, etc.) and overexpressed immune checkpoints (PD-1, PD-L1, LAG3, etc.). These results demonstrated NLRP3 promoted immune escape in cancers. Finally, we investigated the expression of various immune checkpoints by treating NLRP3 inhibitor MCC950 during the co-culture of peripheral blood mononuclear cells (PBMC) and LIHC cell line Hep3B. MCC950 significantly repressed the expression of PD-L1 and LAG3, and promoted the apoptosis rate of Hep3B. In conclusion, our research demonstrated the role of NLRP3 in pan-cancer, especially in LIHC. Inhibition of NLRP3 promoted the killing effect of T cells to cancer cells by repressing the expression of immune checkpoints.
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http://dx.doi.org/10.1016/j.intimp.2021.108512DOI Listing
January 2022

Value of Neutrophil to Lymphocyte Ratio, Platelet to Lymphocyte Ratio, and Red Blood Cell Distribution Width in Evaluating the Prognosis of Children with Severe Pneumonia.

Evid Based Complement Alternat Med 2021 24;2021:1818469. Epub 2021 Sep 24.

Community Health Service Center of Qingdao Licang District, Yongqing Road, Qingdao 266041, Shandong, China.

Objective: To investigate the value of neutrophil to lymphocyte ratio (NLR), platelet to lymphocyte ratio (PLR), and red blood cell distribution width (RDW) in evaluating the prognosis of children with severe pneumonia (SP).

Methods: A retrospective analysis of the data of 91 children with SP admitted to our hospital from March 2018 to March 2021. According to the survival status after 28 days of treatment, all children were divided into the survival group ( = 59) and the death group ( = 32). The clinical data and laboratory indicators of the patients were recorded. Multivariate logistic regression was used to analyze the risk factors of prognosis, and the ROC curve was used to analyze the predictive value of each index.

Results: The Acute Physiology and Chronic Health Evaluation II (APACHE II) score and CURB-65 score of the death group were higher than those of the survival group ( < 0.05). The RDW, NLR, PLR, and high-sensitivity C-reactive protein, procalcitonin blood lactic acid (Lac) of the death group, were higher than those of the survival group, and LYM was lower than the survival group ( < 0.05). Multivariate regression analysis showed that APACHE II score, RDW, NLR, PLR, and Lac were all independent risk factors for poor prognosis in children with SP ( < 0.05). The AUC of NLR, PLR, and RDW for evaluating the prognosis of children with SP were 0.798, 0.781, and 0.777, respectively. The sensitivity was 56.25%, 90.63%, and 56.25%, respectively, and the specificity was 89.83%, 55.93%, and 91.53%, respectively. The AUC of NLR, PLR, and RDW combined to evaluate the prognosis of children with SP was 0.943. When the best cut-off value was 0.8528, the sensitivity was 93.75%, and the specificity was 91.53%.

Conclusion: NLR, PLR, and RDW have certain predictive value for the prognosis of children with SP; the combination of the three indicators has a higher value in evaluating the prognosis of children with SP, which can better guide the prognostic treatment.
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http://dx.doi.org/10.1155/2021/1818469DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8486541PMC
September 2021

Molecular Mechanistic Insights into the Ionic-Strength-Controlled Interfacial Behavior of Proteins on a TiO Surface.

Langmuir 2021 10 22;37(39):11499-11507. Epub 2021 Sep 22.

Energy Engineering, Division of Energy Science, Luleå University of Technology, 97187 Luleå, Sweden.

By adjusting the ionic strengths through changing the concentration of the buffer ions, the molecular force and the interfacial behavior of cytochrome (Cyt ) and TiO are systematically studied. The molecular forces determined by combining the adhesion force and adsorption capacity are found to first increase and then decrease with the increasing ionic strength, with a peak obtained at an ionic strength between 0.8 and 1.0 M. The mechanism is explained based on the dissociation and hydration of ions at the interfaces, where the buffer ions could be completely dissociated at ionic strengths of <0.8 M but were partially associated when the ionic strength increased to a high value (>1.2 M), and the strongest hydration was observed around 1.0 M. The hydrodynamic size and the zeta potential value representing the effective contact area and protein stability of the Cyt molecule, respectively, are also affected by the hydration and are proportional to the molecular forces. The interfacial behavior of Cyt molecules on the TiO surface, determined through surface-enhanced Raman scattering (SERS), is extremely affected by the ionic strength of the solution as the ion dissociation and hydration also increase the electron transfer ability, where the best SERS enhancement is observed at the ionic strength of around 1.0 M, corresponding to the largest molecular force. Our results provide a detailed understanding at the nanoscale on controlling the protein interfacial behavior with solid surfaces, adjusted by the buffer ions.
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http://dx.doi.org/10.1021/acs.langmuir.1c01726DOI Listing
October 2021

Hydrated Ionic Liquids Boost the Trace Detection Capacity of Proteins on TiO Support.

Langmuir 2021 04 16;37(16):5012-5021. Epub 2021 Apr 16.

Energy Engineering, Division of Energy Science, Luleå University of Technology, 97187 Luleå, Sweden.

Trace detection based on surface-enhanced Raman scattering (SERS) has attracted considerable attention, and exploiting efficient strategies to stretch the limit of detection and understanding the mechanisms on molecular level are of utmost importance. In this work, we use ionic liquids (ILs) as trace additives in a protein-TiO system, allowing us to obtain an exceptionally low limit of detection down to 10 M. The enhancement factors (EFs) were determined to 2.30 × 10, 6.17 × 10, and 1.19 × 10, for the three systems: one without ILs, one with ILs in solutions, and one with ILs immobilized on the TiO substrate, respectively, corresponding to the molecular forces of 1.65, 1.32, and 1.16 nN quantified by the atomic force microscopy. The dissociation and following hydration of ILs, occurring in the SERS system, weakened the molecular forces but instead improved the electron transfer ability of ILs, which is the major contribution for the observed excellent detection. The weaker diffusion of the hydrated IL ions immobilized on the TiO substrate did provide a considerably greater EF value, compared to the ILs in the solution. This work clearly demonstrates the importance of the hydration of ions, causing an improved electron transfer ability of ILs and leading to an exceptional SERS performance in the field of trace detection. Our results should stimulate further development to use ILs in SERS and related applications in bioanalysis, medical diagnosis, and environmental science.
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http://dx.doi.org/10.1021/acs.langmuir.1c00525DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154861PMC
April 2021

Nitrogen transformation and pathways in the shallow groundwater-soil system within agricultural landscapes.

Environ Geochem Health 2021 Jan 30;43(1):441-459. Epub 2020 Sep 30.

State Key Laboratory of Nuclear Resources and Environment, East China University of Technology, Nanchang, 330013, China.

The present study considers the behavior of nitrogen compounds in the shallow groundwater-soil system as necessary for the functioning of the nitrogen cycle within agricultural landscapes and one of the first steps of the formation of groundwater chemical composition. Data were collected in 2011-2018 within the Poyang Lake area (Jiangxi Province, China), where agricultural landscapes prevail. The soil and groundwater samples were taken in different periods of an agricultural season at the beginning of the agricultural season (spring) and after harvesting (autumn). The combined geochemical data on the chemical and microbiological composition of the soils and shallow groundwater and isotopic data on dissolved nitrate allowed researchers to resolve that nitrogen enters the system in the form of organic compounds, particularly, due to the soil fertilization at the beginning of the agricultural season. Organic nitrogen compounds transform into nitrate under the influence of nitrifiers in the soil before getting the shallow aquifer, where the occurrence of denitrification is suggested. Within the Ganjiang and Xiushui interfluve, reducing conditions, together with the formation of clay minerals from the aqueous solution, may serve a geochemical barrier for the accumulation of nitrogen compounds preventing the transformation of ammonium to nitrate and providing its sorption. It also should be noted that bacterial diversity in the shallow groundwater has a strong relation with the amount of nitrate in the system, whereas in the soil, it is connected with sampling depth.
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http://dx.doi.org/10.1007/s10653-020-00733-wDOI Listing
January 2021

Excellent Protein Immobilization and Stability on Heterogeneous C-TiO Hybrid Nanostructures: A Single Protein AFM Study.

Langmuir 2020 08 29;36(31):9323-9332. Epub 2020 Jul 29.

State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing 210009, P.R. China.

Enhancing molecular interaction is critical for improving the immobilization and stability of proteins on TiO surfaces. In this work, mesoporous TiO materials with varied pore geometries were decorated with phenyl phosphoric acid (PPA), followed by a thermal treatment to obtain chemically heterogeneous C-TiO samples without changing the geometry and crystalline structure, which can keep the advantages of both carbon and TiO. The molecular interaction force between the protein and the surfaces was measured using atomic force microscopy by decomposing from the total adhesion forces, showing that the surface chemistry determines the interaction strength and depends on the amount of partial carbon coverage on the TiO surface (∼40-80%). Samples with 58.3% carbon coverage provide the strongest molecular interaction force, consistent with the observation from the detected friction force. Surface-enhanced Raman scattering and electrochemical biosensor measurements for these C-TiO materials were further conducted to illustrate their practical implications, implying their promising applications such as in protein detection and biosensing.
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http://dx.doi.org/10.1021/acs.langmuir.0c01942DOI Listing
August 2020

Mechanistic Study of Protein Adsorption on Mesoporous TiO in Aqueous Buffer Solutions.

Langmuir 2019 08 16;35(34):11037-11047. Epub 2019 Aug 16.

State Key Laboratory of Materials-Oriented and Chemical Engineering , Nanjing Tech University , Nanjing 210009 , China.

Protein adsorption is of fundamental importance for bioseparation engineering applications. In this work, a series of mesoporous TiO with various geometric structures and different aqueous buffer solutions were prepared as platforms to investigate the effects of the surface geometry and ionic strength on the protein adsorptive behavior. The surface geometry of the TiO was found to play a dominant role in the protein adsorption capacity when the ionic strength of buffer solutions is very low. With the increase in ionic strength, the effect of the geometric structure on the protein adsorption capacity reduced greatly. The change of ionic strength has the highest significant effect on the mesoporous TiO with large pore size compared with that with small pore size. The interaction between the protein and TiO measured with atomic force microscopy further demonstrated that the adhesion force induced by the surface geometry reduced with the increase in the ionic strength. These findings were used to guide the detection of the retention behavior of protein by high-performance liquid chromatography, providing a step forward toward understanding the protein adsorption for predicting and controlling the chromatographic separation of proteins.
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http://dx.doi.org/10.1021/acs.langmuir.9b01354DOI Listing
August 2019

Determination of the small amount of proteins interacting with TiO nanotubes by AFM-measurement.

Biomaterials 2019 02 20;192:368-376. Epub 2018 Nov 20.

State Key Laboratory of Materials-Oriented and Chemical Engineering and Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, Nanjing 210009, China. Electronic address:

Detecting the small amounts of proteins interacting effectively with the solid film electrodes surface still remains a challenge. To address this, in this work, a new approach was proposed by the combination of the adhesion forces and the molecular interaction measured with AFM. Cytochrome c (Cyt C) interacting effectively with TiO nanotube arrays (TNAs) was chosen as a probe. The amounts of Cyt C molecules interacting effectively on TNAs surface (C) range from 5.5×10 to 7.0×10 mol/cm (68.2-86.8 ng/cm) and they are comparable with the values obtained by the electrochemistry method in the literature, in evidence of the accuracy of this AFM-based approach. The reliability of the proposed approach was further verified by conducting Surface Enhanced Raman Scattering (SERS) measurements and estimating the enhancement factor (EF). This interaction-based AFM approach can be used to accurately obtain the small amounts of adsorbed substances on the solid film electrodes surface in the applications such as biosensors, biocatalysis, and drug delivery, etc.
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http://dx.doi.org/10.1016/j.biomaterials.2018.11.013DOI Listing
February 2019

Adhesion and friction forces in biofouling attachments to nanotube- and PEG- patterned TiO surfaces.

Colloids Surf B Biointerfaces 2017 Nov 27;159:108-117. Epub 2017 Jul 27.

Wuxi Weifu Environment Catalysts Co., LTD, Wuxi 214028, PR China.

The TiO nanotube pattern with features down to 20nm (TN20) is highly and efficiently resistant to fibrinogen and S. aureus attachment. The ability of TN20 to resist biofouling adsorption, is due to low biofouling-surface adhesion force that determines the initial biofouling attachment, as well as the low friction coefficient that enables a complete removal of biofouling from a low-adhesive 'repelling' TN20 substrate under fluid flow. By grafting PEG molecules onto TN20, a significantly higher S. aureus cells attachment was observed, because of the stronger adhesion forces originated from the deformation of the soft PEG coatings. The complete interaction of S. aureus on structure-free dense TiO (DT), yields larger contact area and thus higher adhesion force than on any other TiO surfaces, resulting in a high coverage of bacteria. The existing high friction coefficient of S. aureus on TN80 (TiO with 80nm nanotubular size) and TN80-P (PEG-modified TN80), due to the much greater surface roughness, would contribute to the immobilization of biofouling on the surface under fluid flow, even though the two surfaces exhibit low adhesion forces. The analysis of adhesion and friction forces manipulated by TiO nanotubular topography and posted PEG patterns, advances our understanding of the mechanisms by which nanotopography patterned surfaces reduce biofouling attachment.
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http://dx.doi.org/10.1016/j.colsurfb.2017.07.067DOI Listing
November 2017

Molecular Interactions of Protein with TiO by the AFM-Measured Adhesion Force.

Langmuir 2017 10 17;33(42):11626-11634. Epub 2017 Aug 17.

State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University , Nanjing 210009, P. R. China.

Understanding the interactions between porous materials and biosystems is of great important in biomedical and environmental sciences. Upon atomic force microscopy (AFM) adhesion measurement, a new experimental approach was presented here to determine the molecular interaction force between proteins and mesoporous TiO of various surface roughnesses. The interaction force between each protein molecule and the pure anatase TiO surface was characterized by fitting the adhesion and adsorption capacity per unit contact area, and it was found that the adhesion forces were approximately 0.86, 2.63, and 4.41 nN for lysozyme, myoglobin, and BSA, respectively. Moreover, we reported that the molecular interaction force was independent of the surface topography of the material but the protein type is a factor of the interaction. These experimental results on the molecular level provide helpful insights for stimulating model calculation and molecular simulation studies of protein interaction with surfaces.
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http://dx.doi.org/10.1021/acs.langmuir.7b02024DOI Listing
October 2017

Adhesion and friction in polymer films on solid substrates: conformal sites analysis and corresponding surface measurements.

Soft Matter 2017 May 19;13(19):3492-3505. Epub 2017 Apr 19.

Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695, USA.

In this work, we present a statistical mechanical analysis to elucidate the molecular-level factors responsible for the static and dynamic properties of polymer films. This analysis, which we term conformal sites theory, establishes that three dimensionless parameters play important roles in determining differences from bulk behavior for thin polymer films near to surfaces: a microscopic wetting parameter, α, defined as the ratio of polymer-substrate interaction to polymer-polymer interaction; a dimensionless film thickness, H*; and dimensionless temperature, T*. The parameter α introduced here provides a more fundamental measure of wetting than previous metrics, since it is defined in terms of intermolecular forces and the atomic structure of the substrate, and so is valid at the nanoscale for gas, liquid or solid films. To test this theoretical analysis, we also report atomic force microscopy measurements of the friction coefficient (μ), adhesion force (F) and glass transition temperature (T) for thin films of two polymers, poly(methyl methacrylate) (PMMA) and polystyrene (PS), on two planar substrates, graphite and silica. Both the friction coefficient and the glass transition temperature are found to increase as the film thickness decreases, and this increase is more pronounced for the graphite than for the silica surface. The adhesion force is also greater for the graphite surface. The larger effects encountered for the graphite surface are attributed to the fact that the microscopic wetting parameter, α, is larger for graphite than for silica, indicating stronger attraction of polymer chains to the graphite surface.
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http://dx.doi.org/10.1039/c7sm00261kDOI Listing
May 2017

Efficient nanobiocatalytic systems of nuclease P1 immobilized on PEG-NH2 modified graphene oxide: effects of interface property heterogeneity.

Colloids Surf B Biointerfaces 2016 Sep 27;145:785-794. Epub 2016 May 27.

State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, No. 5, Xinmofan Road, Nanjing, 210009, PR China; College of Biotechnology and Pharmaceutical Engineering, National Engineering Technique Research Center for Biotechnology, Nanjing Tech University, No. 30, Puzhu South Road, Nanjing 211816, PR China; Synergetic Innovation Center for Advanced Materials, Nanjing Tech University, No. 30, Puzhu South Road, Nanjing 211816, PR China. Electronic address:

The use of graphene oxide (GO) nanosheets for functional enzyme support has attracted intensive interest owing to their unique planar structure and intriguing physical and chemical properties. However, the detailed effects of the interface properties of GO and its functionalized derivatives on active biomolecules are not well understood. We immobilize nuclease P1, a common industrial nucleic acid production enzyme, on pristine and amino poly(ethylene glycol) (PEG-NH2) modified GO nanosheets with interface property heterogeneity using two approaches, physical adsorption and chemical crosslinking. It is demonstrated that nuclease P1 could be stable immobilized on the surface of pristine GO by physical adsorption and on the edge of modified GO nanosheets by chemical crosslinking. The resultant loading capacity of nuclease P1 on pristine GO is as high as 6.45mg/mg as a consequence of strong electrostatic and hydrophobic interactions between the enzyme and carrier. However, it is determined that the acid resistance, thermal stability, reusability and degradation efficiency of the immobilized enzyme on PEG-NH2-modified GO are obviously improved compared to those of the enzyme immobilized on pristine GO. The enhanced catalytic behavior demonstrates that GO and its derivatives have great potential in efficient biocatalytic systems.
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http://dx.doi.org/10.1016/j.colsurfb.2016.05.074DOI Listing
September 2016

Bovine Serum Albumin Adsorption in Mesoporous Titanium Dioxide: Pore Size and Pore Chemistry Effect.

Langmuir 2016 04 13;32(16):3995-4003. Epub 2016 Apr 13.

Jiangnan Graphene Research Institute, Changzhou 213149, China.

Understanding the mechanism of protein adsorption and designing materials with high sensitivity, high specificity and fast response are critical to develop the next-generation biosensing and diagnostic platforms. Mesoporous materials with high surface area, tunable pores, and good thermal/hydrostatic stabilities are promising candidates in this field. Because of the excellent biocompatibility, titanium dioxide has received an increasing interest in the past decade for biomedical applications. In this work, we synthesized mesoporous titanium dioxide with controlled pore sizes (7.2-28.0 nm) and explored their application for bovine serum albumin (BSA) adsorption. Scanning electron microscopy (SEM), X-ray powder diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and nitrogen adsorption/desorption experiments were performed to characterize the mesoporous TiO2 samples before and after BSA adsorption. Isothermal microcalorimetry was applied to measure both the adsorption heat and conformation rearrangement heat of BSA in those mesopores. We also carried out thermogravimetry measurements to qualitatively estimate the concentration of hydroxyl groups, which plays an important role in stabilizing BSA in-pore adsorption. The adsorption stability was also examined by leaching experiments. The results showed that TiO2 mesopores can host BSA adsorption when their diameters are larger than the hydrodynamic size of BSA (∼9.5 nm). In larger mesopores studied, two BSA molecules were adsorbed in the same pores. In contrast to the general understanding that large mesopores demonstrate poor stabilities for protein adsorptions, the synthesized mesoporous TiO2 samples demonstrated good leaching stabilities for BSA adsorption. This is probably due to the combination of the mesoporous confinement and the in-pore hydroxyl groups.
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http://dx.doi.org/10.1021/acs.langmuir.5b04496DOI Listing
April 2016

A cost-effective system for in-situ geological arsenic adsorption from groundwater.

J Contam Hydrol 2013 Nov 28;154:1-9. Epub 2013 Aug 28.

State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan 430074, China; School of Environmental Studies, China University of Geosciences, Wuhan 430074, China.

An effective and low-cost in-situ geological filtration system was developed to treat arsenic-contaminated groundwater in remote rural areas. Hangjinhouqi in western Hetao Plain of Inner Mongolia, China, where groundwater contains a high arsenic concentration, was selected as the study area. Fe-mineral and limestone widely distributed in the study area were used as filter materials. Batch and column experiments as well as field tests were performed to determine optimal filtration parameters and to evaluate the effectiveness of the technology for arsenic removal under different hydrogeochemical conditions. A mixture containing natural Fe-mineral (hematite and goethite) and limestone at a mass ratio of 2:1 was found to be the most effective for arsenic removal. The results indicated that Fe-mineral in the mixture played a major role for arsenic removal. Meanwhile, limestone buffered groundwater pH to be conducive for the optimal arsenic removal. As(III) adsorption and oxidation by iron mineral, and the formation of Ca-As(V) precipitation with Ca contributed from limestone dissolution were likely mechanisms leading to the As removal. Field demonstrations revealed that a geological filter bed filled with the proposed mineral mixture reduced groundwater arsenic concentration from 400 μg/L to below 10 μg/L. The filtration system was continuously operated for a total volume of 365,000L, which is sufficient for drinking water supplying a rural household of 5 persons for 5 years at a rate of 40 L per person per day.
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http://dx.doi.org/10.1016/j.jconhyd.2013.08.002DOI Listing
November 2013

Educational web-based intervention for high school students to increase knowledge and promote positive attitudes toward organ donation.

Health Educ Behav 2006 Dec 21;33(6):773-86. Epub 2006 Aug 21.

Institute for Social Research, University of Michigan, Ann Arbor, Michigan 48106-1248, USA.

A sample of 490 high school students from 81 schools in Michigan participated in an experiment in which they were randomly assigned to either a control or an experimental Web site. The experimental Web site provided exposure to educational material about the process of organ donation and organ transplantation. The control Web site provided educational material on methods to avoid the common cold. The pre-and posttests of knowledge of issues related to organ donation and of attitude toward donation demonstrated statistically significant increases for the experimental group compared with the control group. A structural equation path model suggested that these increases in knowledge and prodonation attitude mediated the effects of the experiment on contacting the Michigan donor registry. The increase in knowledge and in prodonation attitude increased the likelihood of contacting the registry. The potential for this and similar other Web interventions to enhance students' health education is discussed.
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http://dx.doi.org/10.1177/1090198106288596DOI Listing
December 2006

Internet-based intervention to promote organ donor registry participation and family notification.

Transplantation 2003 Apr;75(8):1175-9

Department of Surgery, University of Michigan, Ann Arbor, MI 48109-0331, USA.

Background: Little is known regarding the potential of Internet-based educational interventions to increase organ donor registry participation and family notification of donation wishes. We studied the effects of an Internet-based multimedia intervention (www.journey.transweb.org) on donor registry participation and family notification.

Methods: Visitors to a specially designed web site were studied between December 14, 2000, and March 31, 2002. Demographic characteristics were requested, and a pretest was administered to one half of the participants (selected randomly) before web site content exposure. All visitors were offered a posttest. Eight knowledge questions (true/false), three attitude questions (7-point scale), and three behaviors (yes/no) were assessed.

Results: A total of 10,884 visitors provided demographic data. Correct answers to knowledge questions increased from 85.1% to 87.0% overall (pretest vs. posttest; P<0.001) and from 80.6% to 82.0% for teenagers (P<0.001). Willingness to donate increased (scores of 6.34 vs. 6.39; P<0.001), as did willingness to join a donor registry (scores of 5.53 vs. 5.67; P<0.001). Willingness measures were less positive among teenagers but increased significantly after exposure to the intervention. Almost 10% of visitors directly linked to an online registry and 2,489 (23%) used the web site facilities to communicate donation wishes. Increases in knowledge were not associated with changes in attitudes, but an increase in pro-donation attitude was a significant predictor of donor registry participation (P<0.001).

Conclusions: Increases in donation attitudes among visitors to an organ donation web site resulted in positive behaviors, such as enrollment in a donor registry and family notification. Future efforts should focus on using Internet-based interventions to improve attitudes toward donation and to facilitate pro-donation behaviors.
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http://dx.doi.org/10.1097/01.TP.0000062845.51042.DDDOI Listing
April 2003
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