Publications by authors named "Yihan Wang"

159 Publications

Novel Hybrid p- and n-Type Organic Thermoelectric Materials Based on Mussel-Inspired Polydopamine.

ACS Appl Mater Interfaces 2021 May 11. Epub 2021 May 11.

College of Electrical Engineering, Sichuan University, Chengdu 610064, China.

Mussel-inspired polydopamine (PDA) can serve as building blocks and interfaces in designing functional materials. Here, the use of PDA as an interlayer between polyaniline (PANi) and multidimensional carbon materials, such as graphene quantum dots (GQD), multiwalled carbon nanotubes (MWCNT), and graphene nanosheets (GNS), to improve the thermoelectric performance of p-type polymer-based materials has been reported. The introduction of PDA promotes the carrier mobility of GQD/PDA/PANi, CNT/PDA/PANi, and GNS/PDA/PANi ternary composites due to the superior adhesive property of PDA. An optimal conductivity of 4.98 × 10 S m and a power factor of 92.17 μW m K at 363 K are achieved in GNS/PDA/PANi, which are much higher than the values of GNS/PDA and GNS/PANi. More surprisingly, despite the fact that GQD/PDA, CNT/PDA, and GNS/PDA binary composites show p-type properties, the pyrolysis treatment of GQD/PDA, CNT/PDA, and GNS/PDA at 800 °C results in a gain in both the electrical conductivity and negative Seebeck coefficient of c-GQD/PDA, c-CNT/PDA, and c-GNS/PDA. The c-CNT/PDA composites possess the highest Seebeck value of -30.2 μV K and a maximum power factor value of 35.57 μW m K. Finally, a flexible thermoelectric generator with 24 thermoelectric units composed of GNS/PDA/PANi and c-CNT/PDA is demonstrated, which gives an output voltage of 52.8 mV at a temperature difference of 60 °C.
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http://dx.doi.org/10.1021/acsami.1c01457DOI Listing
May 2021

Comparative Analysis of piRNA Profiles Helps to Elucidate Cryoinjury Between Giant Panda and Boar Sperm During Cryopreservation.

Front Vet Sci 2021 22;8:635013. Epub 2021 Apr 22.

College of Animal Sciences and Technology and Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China.

Cryopreservation induces sperm cryoinjuries, including physiological and functional changes. However, the molecular mechanisms of sperm cryoinjury and cryoresistance are still unknown. Cryoresistance or the freeze tolerance of sperm varies across species, and boar sperm is more susceptible to cold stress. Contrary to boar sperm, giant panda sperm appears to be strongly freeze-tolerant and is capable of surviving repeated cycles of freeze-thawing. In this study, differentially expressed (DE) PIWI-interacting RNAs (piRNAs) of fresh and frozen-thawed sperm with different freeze tolerance capacity from giant panda and boar were evaluated. The results showed that 1,160 (22 downregulated and 1,138 upregulated) and 384 (110 upregulated and 274 downregulated) DE piRNAs were identified in giant panda and boar sperm, respectively. Gene ontology (GO) enrichment analysis revealed that the target DE messenger RNAs (mRNAs) of DE piRNAs were mainly enriched in biological regulation, cellular, and metabolic processes in giant panda and boar sperm. Moreover, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that the target DE mRNAs of DE piRNAs were only distributed in DNA replication and the cyclic adenosine monophosphate (cAMP) signaling pathway in giant panda, but the cAMP, cyclic guanosine monophosphate (cGMP), and mitogen-activated protein kinase (MAPK) signaling pathways in boar sperm were considered as part of the olfactory transduction pathway. In conclusion, we speculated that the difference in the piRNA profiles and the DE piRNAs involved in the cAMP signaling pathway in boar and giant panda may have contributed to the different freeze tolerance capacities between giant panda and boar sperm, which helps to elucidate the molecular mechanism behind sperm cryoinjury and cryoresistance.
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http://dx.doi.org/10.3389/fvets.2021.635013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100531PMC
April 2021

Effect of storage states on stability of three organophosphorus insecticide residues on cowpea samples.

J Sci Food Agric 2021 Apr 15. Epub 2021 Apr 15.

College of Science, China Agricultural University, Beijing, China.

Background: The stability of pesticide residues in stored samples is very important to ensure the quality of data about the residues. The evaluation of pesticide residues in food and environment samples is an important means to ensure food quality and protect consumers against potential dietary risks. Improper storage of pesticide residue samples may result in loss of pesticide and unreliable data, which could affect safety assessments.

Results: The influences of storage conditions, including temperature (-20 °C, 4 °C, and ambient temperature) and sample state (homogenized state and coarsely chopped state) on the storage stability of dichlorvos, malathion, and diazinon on cowpea were studied. Dichlorvos and malathion were more stable in an homogenized state than in a coarsely chopped state. At 4 °C, the residual dichlorvos in the coarsely chopped state and the homogenized state, respectively, was 12% and 69%; the residual malathion was 26% and 92%, respectively. Dichlorvos suffered a large loss of 89% and 59% for coarsely chopped and homogenized cowpea, even at -20 °C. It was obvious that the stability of dichlorvos and malathion were more affected by storage state than diazinon. The stability of diazinon was significantly affected by temperature. The effect of storage state and temperature on stability is likely to be correlated with enzymes in the matrix, such as superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD).

Conclusion: The optimal stable storage conditions for three organophosphorus insecticides residues on cowpea were in the homogenized state and under a lower temperature. © 2021 Society of Chemical Industry.
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http://dx.doi.org/10.1002/jsfa.11257DOI Listing
April 2021

Odorant and Taste Receptors in Sperm Chemotaxis and Cryopreservation: Roles and Implications in Sperm Capacitation, Motility and Fertility.

Genes (Basel) 2021 Mar 27;12(4). Epub 2021 Mar 27.

College of Animal Science and Technology and Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.

Sperm chemotaxis, which guide sperm toward oocyte, is tightly associated with sperm capacitation, motility, and fertility. However, the molecular mechanism of sperm chemotaxis is not known. Reproductive odorant and taste receptors, belong to G-protein-coupled receptors (GPCR) super-family, cause an increase in intracellular Ca concentration which is pre-requisite for sperm capacitation and acrosomal reaction, and result in sperm hyperpolarization and increase motility through activation of Ca-dependent Cl channels. Recently, odorant receptors (ORs) in olfactory transduction pathway were thought to be associated with post-thaw sperm motility, freeze tolerance or freezability and cryo-capacitation-like change during cryopreservation. Investigation of the roles of odorant and taste receptors (TRs) is important for our understanding of the freeze tolerance or freezability mechanism and improve the motility and fertility of post-thaw sperm. Here, we reviewed the roles, mode of action, impact of odorant and taste receptors on sperm chemotaxis and post-thaw sperm quality.
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http://dx.doi.org/10.3390/genes12040488DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065900PMC
March 2021

Apigenin enhances viability of random skin flaps by activating autophagy.

Phytother Res 2021 Apr 1. Epub 2021 Apr 1.

Department of Orthopaedic Surgery, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.

Random skin flap is widely used in plastic surgery. However, flap necrosis caused by ischemia-reperfusion injury limits its clinical applications. Apigenin, a naturally occurring flavonoid mainly derived from plants, facilitates flap survival. In this study, we explored the effects of apigenin on flap survival and the underlying mechanisms. A total of 54 mice having a dorsal random flap model were randomly divided into control, apigenin, and apigenin +3-methyladenine groups. These groups were treated with dimethyl sulfoxide solution, apigenin, and apigenin +3-methyladenine, respectively. The animals were then euthanized to assess angiogenesis, apoptosis, oxidative stress, and autophagy levels through histological and protein analyses. Apigenin promotes survival of the skin flap area and reduces tissue edema. In addition, apigenin enhanced angiogenesis, attenuated apoptosis, alleviated oxidative stress, and activated autophagy. Interestingly, 3-methyladenine reversed the effects of apigenin on flap survival, angiogenesis, apoptosis, and oxidative stress through inhibition of autophagy. The findings of this study show that apigenin promotes angiogenesis, inhibits cell apoptosis, and lowers oxidative stress by mediating autophagy, thus the improving survival rate of random skin flaps.
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http://dx.doi.org/10.1002/ptr.7090DOI Listing
April 2021

Intratumor heterogeneity of breast cancer detected by epialleles shows association with hypoxic microenvironment.

Theranostics 2021 4;11(9):4403-4420. Epub 2021 Mar 4.

State Key Laboratory of Respiratory Disease, Guangzhou Medical University, Guangzhou, 51000, China.

In breast cancer, high intratumor DNA methylation heterogeneity can lead to drug-resistant, metastasis and poor prognosis of tumors, which increases the complexity of cancer diagnosis and treatment. However, most studies are limited to average DNA methylation level of individual CpGs and ignore heterogeneous DNA methylation patterns of cell subpopulations within the tumor. Thus, quantifying the variability in DNA methylation pattern in sequencing reads is valuable for understanding intratumor heterogeneity. We performed Reduced Representation Bisulfite Sequencing and RNA sequencing for tumor core and tumor periphery regions within one breast tumor. By developing a method named "epialleJS" based on Jensen-Shannon divergence, we detected the differential epialleles between tumor core and tumor periphery (CPDEs). We then explored the correlation between intratumor methylation heterogeneity and hypoxic microenvironment in TCGA breast cancer cohort. More than 70% of CPDEs had higher epipolymorphism in tumor core than tumor periphery, and these CPDEs had lower methylation in tumor core. The CPDEs with lower methylation in tumor core may associate with hypoxic tumor microenvironment. Moreover, we identified a signature of five hypoxia-related DNA methylation markers which can predict the prognosis of breast cancer patients, including a CpG site cg15190451 in gene . Furthermore, immunohistochemical analysis confirmed that the expression of was associated with clinicopathological characteristics and survival of breast cancer patients. The analysis of intratumor DNA methylation heterogeneity based on epialleles reveals that disordered methylation patterns in tumor core are associated with hypoxic microenvironment, which provides a framework for understanding biological heterogeneous behavior and guidance for developing effective treatment schemes for breast cancer patients.
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http://dx.doi.org/10.7150/thno.53737DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7977462PMC
March 2021

The effects of histone crotonylation and bromodomain protein 4 on prostate cancer cell lines.

Transl Androl Urol 2021 Feb;10(2):900-914

Department of Urology, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Background: The aims of this study were to detect the level of histone crotonylation in prostate cancer (PCa) tissues, analyze the correlations between its level and clinical stage and grade, and explore the effects of bromodomain-containing protein 4 (BRD4) inhibitors and sodium crotonate on the histone crotonylation in PCa cell lines and on the functions of PCa cells.

Methods: PCa tissues from 72 patients and adjacent tissues from 7 patients were collected, and immunohistochemistry was used to measure the level of histone crotonylation. Three human PCa cell lines, PC-3, LNCaP, and C42B, were selected and treated with IC50 value of I-BET762, I-BET726, and CPI-203, respectively. Next, short hairpin RNA (shRNA) transient knockdown was used to inhibit BRD4 expression. Histone crotonylation level and the expression of acetylase were determined by Western blotting. Finally, cell proliferation, migration, and invasion were measured with Cell Counting Kit-8 assay, scratch test, and Transwell test respectively.

Results: The level of histone crotonylation in PCa tissue was higher than that in adjacent tissues, and histone lysine crotonylation (Kcr) increased with the increasing malignancy of PCa. Treatments with I-BET762, I-BET726, and CPI-203 could inhibit the proliferation, migration, and invasion of PCa cell lines including PC-3, LNCaP, and C42B, and could also regulate the histone crotonylation and androgen receptor signaling pathways via the regulation of BRD4 expression.

Conclusions: PCa is closely related to histone crotonylation. Inhibition of BRD4 expression can inhibit the proliferation, migration, and invasion of PCa cells.
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http://dx.doi.org/10.21037/tau-21-53DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947446PMC
February 2021

Pathogenesis study based on high throughput single-cell sequencing analysis reveals novel transcriptional landscape and heterogeneity of retinal cells in type 2 diabetic mice.

Diabetes 2021 Mar 5. Epub 2021 Mar 5.

Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University, Shanghai, 20080, China

Diabetic retinopathy (DR) is the leading cause of acquired blindness in middle-aged people. The complex pathology of DR is difficult to dissect, given the convoluted cytoarchitecture of the retina. Here, we performed single-cell RNA sequencing (scRNA-seq) of retina from type 2 diabetic model induced in leptin receptor-deficient (db/db) and control db/m mice with the aim of elucidating the factors mediating the pathogenesis of DR. We identified eleven cell types and determined cell type-specific expression of DR-associated loci via genome-wide association study-based enrichment analysis. DR also impacted cell type-specific genes and altered cell-cell communication. Based on the scRNA-seq results, retinaldehyde-binding protein 1 (RLBP1) was investigated as a promising therapeutic target for DR. Retinal RLBP1 expression was decreased in diabetes, and its overexpression in Müller glia mitigated DR-associated neurovascular degeneration. These data provide a detailed analysis of the retina under diabetic and normal conditions, revealing new insights into pathogenic factors that may be targeted to treat DR and related dysfunctions.
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http://dx.doi.org/10.2337/db20-0839DOI Listing
March 2021

CaO/gelatin oxygen slow-releasing microspheres facilitate tissue engineering efficiency for the osteonecrosis of femoral head by enhancing the angiogenesis and survival of grafted bone marrow mesenchymal stem cells.

Biomater Sci 2021 Apr 2;9(8):3005-3018. Epub 2021 Mar 2.

Department of Spinal Surgery, Orthopedic Medical Center, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China.

The osteonecrosis of femoral head (ONFH), a common refractory disease, is still not fully understood today. Hypoxia caused by ischemia is not only an important pathogenic factor but also a critical challenge for the survival of seed cells in the tissue engineering therapy of ONFH. To explore an efficient strategy to treat ONFH by targeting hypoxia, newly designed CaO/gelatin microspheres were composited with 3D printed polycaprolactone/nano-hydroxyapatite (PCL/nHA) porous scaffold, sodium alginate/gelatin hydrogel, and bone marrow mesenchymal stem cells (BMSCs) to develop a novel tissue engineering scaffold and then transplanted into the core depression area of the ONFH rabbit model. The current data demonstrated that CaO/gelatin microspheres can constantly release oxygen for 19 days. In vitro assays with BMSCs illustrated that scaffolds have high biocompatibility and are favorable for cell proliferation in extreme hypoxia (1% O). The in vivo study demonstrated that the transplanted scaffold with oxygen-generating microspheres significantly enhanced the osteogenic and angiogenic effects compared to the scaffold without microspheres. Further assessments revealed that microspheres in the scaffold can reduce the local cell apoptosis and enhance the survival of grafted cells in the host. Collectively, the present study developed a novel oxygen slow-releasing composite scaffold, which can facilitate tissue engineering efficiency for treating the osteonecrosis of the femoral head by enhancing the angiogenesis and survival of grafted stem cells.
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http://dx.doi.org/10.1039/d0bm02071kDOI Listing
April 2021

Resveratrol promotes lysosomal function via ER calcium-dependent TFEB activation to ameliorate lipid accumulation.

Biochem J 2021 Mar;478(5):1159-1173

Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, China.

Abnormal lipid accumulation is associated to the development of metabolic diseases such as hepatic steatosis and lipid storage diseases. Pharmacological agents that can attenuate lipid accumulation therefore have therapeutic potentials for these diseases. Resveratrol (RSV), a natural active substance found in fruits and nuts, has been reported to effectively reduce the intracellular lipid accumulation, but the underlying mechanisms of RSV remain elusive. Here, we show that RSV triggers an endoplasmic reticulum (ER)- Ca2+ signaling that activates transcriptional factor EB (TFEB), a master transcriptional regulator of autophagic and lysosomal biogenesis. Moreover, RSV activates protein phosphatase 2A (PP2A), which binds and dephosphorylates TFEB, promoting its nuclear translocation and the expression of TFEB target genes required for autophagosome and lysosomal biogenesis. Notably, genetic inhibition of TFEB significantly ameliorates RSV-mediated lipid clearance. Taken together, these data link RSV-induced ER calcium signaling, PP2A and TFEB activation to promote autophagy and lysosomal function, by which RSV may trigger a cellular self-defense mechanism that effectively mitigate lipid accumulation commonly associated with many metabolic diseases.
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http://dx.doi.org/10.1042/BCJ20200676DOI Listing
March 2021

Nonlinear iterative perturbation scheme with simplified spherical harmonics (SP ) light propagation model for quantitative photoacoustic tomography.

J Biophotonics 2021 Feb 12:e202000446. Epub 2021 Feb 12.

School of Life Science and Technology, Xidian University, Xi'an, China.

When using quantitative photoacoustic tomography (q-PAT) reconstruction to recover the optical absorption coefficients of tissue, the commonly used diffusion equation has several limitations in the case of the objects that have small geometries and high-absorption or low-scattering areas. Furthermore, the conventional perturbation reconstruction strategy is unsatisfactory when the target tissue containing large heterogeneous features. We herein present a modified q-PAT implementation that employs the higher-order photon migration model achieving the tradeoff between mathematical rigidity and computational efficiency. Besides, a nonlinear iterative method is proposed to obtain the perturbations of optical absorption considering the updating of the sensitivity matrix in calculating the fluence perturbations. Consequently, the distribution of tissue optical properties can be recovered in a robust way even if the targets with high absorption are included. The proposed approach has been validated by simulation, phantom and in vivo experiments, exhibiting promising performances in image fidelity and quantitative feasibility for practical applications.
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http://dx.doi.org/10.1002/jbio.202000446DOI Listing
February 2021

Data on flexibility and thermal stability of Polypyrrole-based ternary nanocomposite films.

Data Brief 2021 Feb 14;34:106754. Epub 2021 Jan 14.

College of Electrical Engineering, Sichuan University, Chengdu 610064, China.

Carbon materials, such as multi-walled carbon nanotube (MWCNT), single-walled carbon nanotubes (SWCNT), and graphene sheets (GNS), filling into polymer substrates can effectively improve performance of composite materials [1], [2]. The data presented here in this article illustrates the different impacts of GNS and MWCNT on the mechanical properties of polypyrrole (PPy)-based composites systems. PPy/GNS and PPy/MWCNT binary composites were added into poly(3,4-ethylenedioxythiophene): poly (styrene sulfonate) (PEDOT: PSS) matrix. Changing the ratio of PPy/GNS and PPy/MWCNT to PEDOT: PSS, a series of PEDOT: PSS-PPy/GNS (abbreviated as PGNS) and PEDOT: PSS-PPy/MWCNT (abbreviated as PMWCNT) ternary composites films were obtained. The synthesis process of PGNS and PMWCNT films are based on Wang et al [3].
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http://dx.doi.org/10.1016/j.dib.2021.106754DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7843399PMC
February 2021

The Anti-Inflammatory Effect of KS23, A Novel Peptide Derived From Globular Adiponectin, on Endotoxin-Induced Uveitis in Rats.

Front Pharmacol 2020 12;11:585446. Epub 2021 Jan 12.

Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Adiponectin has been shown to exert potent anti-inflammatory activities in a range of systemic inflammatory diseases. This study aimed to investigate the potential therapeutic effects of KS23, a globular adiponectin-derived peptide, on endotoxin-induced uveitis (EIU) in rats and lipopolysaccharide (LPS)-stimulated mouse macrophage-like RAW 264.7 cells. EIU was induced in Lewis rats by subcutaneous injection of LPS into a single footpad. KS23 or phosphate-buffered saline (PBS) was administered immediately after LPS induction via intravitreal injection. Twenty-four hours later, clinical and histopathological scores were evaluated, and the aqueous humor (AqH) was collected to determine the infiltrating cells, protein concentration, and levels of inflammatory cytokines. , cultured RAW 264.7 cells were stimulated with LPS in the presence or absence of KS23, inflammatory cytokine levels in the supernatant, nuclear translocation of nuclear factor kappa B (NF-κB) subunit p65, and the expression of NF-kB signaling pathway components were analyzed. KS23 treatment significantly ameliorated the clinical and histopathological scores of EIU rats and reduced the levels of infiltration cells, protein, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the aqueous humor. Consistently, KS23 decreased the expression of TNF-α and IL-6 in the supernatant of LPS-stimulated RAW 264.7 cells and inhibited the LPS-induced nuclear translocation of NF-κB p65 and the phosphorylation of IKKα/β/IκBα/NF-κB. The and results demonstrated the anti-inflammatory effects of the peptide KS23 and suggested that KS23 is a compelling, novel therapeutic candidate for the treatment of ocular inflammation.
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http://dx.doi.org/10.3389/fphar.2020.585446DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835799PMC
January 2021

The complete chloroplast genome sequence of (Ulmaceae).

Mitochondrial DNA B Resour 2020 Jul 24;5(3):2957-2958. Epub 2020 Jul 24.

College of Life Sciences, Henan Agricultural University, Zhengzhou, China.

Jacq is a kind of landscape tree endemic to East Asia. In this study, the complete chloroplast genome of was sequenced. The genome was 159,259 bp in length, with a large single-copy (LSC) region of 88,451 bp, a small single-copy (SSC) region of 19,598 bp, and two inverted repeat (IR) regions of 25,605 bp, each. The genome consisted of 121 genes, including 77 protein-coding genes, 36 tRNA genes, and 8 rRNA genes. The phylogenetic results indicated that is not monophyletic and constitute a well-supported clade sister to Sect. . The complete chloroplast genome of will provide important information for phylogenetic and evolutionary studies in Ulmaceae, as well as the other closely related families.
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http://dx.doi.org/10.1080/23802359.2020.1791006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782882PMC
July 2020

Cotransplantation of mesenchymal stem cells and endothelial progenitor cells for treating steroid-induced osteonecrosis of the femoral head.

Stem Cells Transl Med 2021 May 13;10(5):781-796. Epub 2021 Jan 13.

Department of Spinal Surgery, Orthopedic Medical Center, Zhujiang Hospital, Southern Medical University, Guangzhou, People's Republic of China.

Steroid-induced osteonecrosis of the femoral head (ONFH) is characterized by decreased osteogenesis, angiogenesis, and increased adipogenesis. While bone tissue engineering has been widely investigated to treat ONFH, its therapeutic effects remain unsatisfactory. Therefore, further studies are required to determine optimal osteogenesis, angiogenesis and adipogenesis in the necrotic area of the femoral head. In our study, we developed a carboxymethyl chitosan/alginate/bone marrow mesenchymal stem cell/endothelial progenitor cell (CMC/ALG/BMSC/EPC) composite implant, and evaluated its ability to repair steroid-induced ONFH. Our in vitro studies showed that BMSC and EPC coculture displayed enhanced osteogenic and angiogenic differentiation. When compared with single BMSC cultures, adipogenic differentiation in coculture systems was reduced. We also fabricated a three-dimensional (3D) CMC/ALG scaffold for loading cells, using a lyophilization approach, and confirmed its good cell compatibility characteristics, that is, high porosity, low cytotoxicity and favorable cell adhesion. 3D coculture of BMSCs and EPCs also promoted secretion of osteogenic and angiogenic factors. Then, we established an rabbit model of steroid-induced ONFH. The CMC/ALG/BMSC/EPC composite implant was transplanted into the bone tunnel of the rabbit femoral head after core decompression (CD) surgery. Twelve weeks later, radiographical and histological analyses revealed CMC/ALG/BMSC/EPC composite implants had facilitated the repair of steroid-induced ONFH, by promoting osteogenesis and angiogenesis, and reducing adipogenesis when compared with CD, CMC/ALG, CMC/ALG/BMSC and CMC/ALG/EPC groups. Thus, our data show that cotransplantation of BMSCs and EPCs in 3D scaffolds is beneficial in treating steroid-induced ONFH.
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http://dx.doi.org/10.1002/sctm.20-0346DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8046137PMC
May 2021

Hierarchical WSe nanoflower as a cathode material for rechargeable Mg-ion batteries.

J Colloid Interface Sci 2021 Apr 28;588:378-383. Epub 2020 Dec 28.

College of Chemistry and Chemical Engineering, Xinyang Normal University,Xinyang 464000, PR China. Electronic address:

Transition metal dichalcogenides (TMDs) have emerged as a promising material in the energy field due to their unique structural arrangement. In this work, ordered flower-like WSe nanosheet was synthesized through simple one-step hydrothermal method, and its cathode application for rechargeable Mg-ion batteries was assessed. The WSe cathode exhibits a high reversible capacity above 265 mAh g at 50 mA g, excellent cycling life of 90% initial capacitance that can be ceaselessly harvested for 100 cycles at 50 mA g, and superior rate capability of 70% initial capacitance maintained even at the current density of 500 mA g. This work paves the way for the application of WSe cathode in Mg-ion and other rechargeable batteries.
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http://dx.doi.org/10.1016/j.jcis.2020.12.083DOI Listing
April 2021

Cyclophilin A inhibits A549 cell oxidative stress and apoptosis by modulating the PI3K/Akt/mTOR signaling pathway.

Biosci Rep 2021 Jan;41(1)

College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China.

The excessive and inappropriate production of reactive oxygen species (ROS) can cause oxidative stress and is implicated in the pathogenesis of lung cancer. Cyclophilin A (CypA), a member of the immunophilin family, is secreted in response to ROS. To determine the role of CypA in oxidative stress injury, we investigated the role that CypA plays in human lung carcinoma (A549) cells. Here, we showed the protective effect of human recombinant CypA (hCypA) on hydrogen peroxide (H2O2)-induced oxidative damage in A549 cells, which play crucial roles in lung cancer. Our results demonstrated that hCypA substantially promoted cell viability, superoxide dismutase (SOD), glutathione (GSH), and GSH peroxidase (GSH-Px) activities, and attenuated ROS and malondialdehyde (MDA) production in H2O2-induced A549 cells. Compared with H2O2-induced A549 cells, Caspase-3 activity in hCypA-treated cells was significantly reduced. Using Western blotting, we showed that hCypA facilitated Bcl-2 expression and inhibited Bax, Caspase-3, Caspase-7, and PARP-1 expression. Furthermore, hCypA activates the PI3K/Akt/mTOR pathway in A549 cells in response to H2O2 stimulation. Additionally, peptidyl-prolyl isomerase activity was required for PI3K/Akt activation by CypA. The present study showed that CypA protected A549 cells from H2O2-induced oxidative injury and apoptosis by activating the PI3K/Akt/mTOR pathway. Thus, CypA might be a potential target for lung cancer therapy.
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http://dx.doi.org/10.1042/BSR20203219DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7846964PMC
January 2021

Characterization of the complete chloroplast genome of (Rhamnaceae).

Mitochondrial DNA B Resour 2020 Jan 31;5(1):934-935. Epub 2020 Jan 31.

College of Forestry, Henan Agricultural University, Zhengzhou, China.

is a widely distributed species with economic, ornamental, and medicinal value in China. In this study, we assembled and characterized the complete chloroplast genome of for the first time. The circular genome has a quadripartite structure with 161,651 bp in length and contains a pair of 26,619 bp inverted repeat (IR) regions, separated by the large single-copy (LSC, 89,443 bp) region and small single-copy (SSC, 18,970 bp) region. The plastid genome harbours 104 unique genes, including 72 protein-coding genes, 28 tRNAs, and four rRNAs. The overall GC content of the whole genome was 36.7%. Further, the phylogenetic analysis showed that clustered together with genus. The complete chloroplast genome of will provide important information for phylogenetic and evolutionary studies in Rhamnaceae, as well as the other closely related family.
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http://dx.doi.org/10.1080/23802359.2020.1714492DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7748519PMC
January 2020

Relationship Between Vitamin D Level and Mortality in Adults With Psoriasis: A Retrospective Cohort Study of NHANES Data.

Clin Ther 2021 Feb 24;43(2):e33-e38. Epub 2020 Dec 24.

Department of Dermatology, Hangzhou Hospital of Traditional Chinese Medicine, Hangzhou, China. Electronic address:

Purpose: Studies have shown an increased risk for mortality in patients with psoriasis. Furthermore, research has demonstrated an inverse relationship between 25-hydroxyvitamin D (25[OH]D) level and all-cause mortality. This study investigated the association between 25(OH)D level and all-cause mortality in US adults with psoriasis.

Methods: Data from NHANES (1999-2014 and mortality data through December 31, 2015) were analyzed. Quartiles of 25(OH)D level were created based on 25(OH)D levels among patients. Cox proportional hazards models were used for estimating hazard ratios (95% CI) for all-cause mortality.

Findings: A total of 82,091 participants were enrolled in the NHANES study from 1999 to 2014. Overall, 610 patients with psoriasis were identified in NHANES. The mean (SD) duration of follow-up was 5.61 (3.38) years (3427.92 person-years). The hazard ratio for mortality in the fully adjusted model was 0.12 (95% CI, 0.02-0.60; P = 0.01) in patients with a high 25(OH)D concentration compared to those with 25(OH)D deficiency.

Implications: The 25(OH)D concentration was significantly inversely associated with all-cause mortality among these patients with psoriasis. Studies have shown an increased risk for mortality in patients with psoriasis compared to the general population. Vitamin D is not regularly metabolized in patients with psoriasis due to their skin abnormality. Vitamin D supplementation has been associated with a reduced mortality in patients with psoriasis. In practice, attention to vitamin D level is crucial, as is the use of vitamin D supplementation, for improving the health of these patients.
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http://dx.doi.org/10.1016/j.clinthera.2020.11.016DOI Listing
February 2021

Electric vehicle-attributed environmental injustice: Pollutant transfer into regions with poor traffic accessibility.

Sci Total Environ 2021 Feb 21;756:143853. Epub 2020 Nov 21.

School of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen 518055, China.

Electric vehicles (EVs) are promoted in recent years as an effective way in alleviating the air pollution caused by tailpipe emissions. However, the pollutants derived from EVs are unheeded. EVs rely on electricity to provide power, and thus their related pollution is transferred to the power plants, which gives rise to the environmental and health pressure to the adjacent regions. In this paper, the transfer of EV-attributed PM, SO, and NO inhalations in China are studied. Then by comparing the inhalations versus traffic accessibility among the impacted municipalities, this study sheds light on the environmental injustice lying in the mismatch between pollutant inhalations and traffic accessibility. The results reveal that compared with Shanghai and Shenzhen, the promotion of EVs in Beijing triggers higher pollutant inhalations to its surrounding municipalities. North China Power Grid undertakes 715.62 g PM inhalation in total, which is 2.51 and 3.20 times higher than the East China Power Grid and the China Southern Power Grid, respectively. The number of municipalities with lower traffic accessibility while higher pollutant inhalation is 8,8, and 17 in North China Power Grid, East China Power Grid, and China Southern Power Grid respectively, indicating conspicuous environmental injustice resulted from the promotion of EVs.
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http://dx.doi.org/10.1016/j.scitotenv.2020.143853DOI Listing
February 2021

1,25(OH)2D3 inhibits osteogenic differentiation through activating β‑catenin signaling via downregulating bone morphogenetic protein 2.

Mol Med Rep 2020 Dec 20;22(6):5023-5032. Epub 2020 Oct 20.

Department of Orthopedics, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, P.R. China.

The present study explored whether bone morphogenetic proteins (BMPs) and Wnt/β‑catenin signaling pathways were involved in the 1,25(OH)2D3‑induced inhibition of osteogenic differentiation in bone marrow‑derived mesenchymal stem cells (BMSCs). To evaluate the osteogenic differentiation of BMSCs, the expression levels of ossification markers, including BMP2, Runt‑related transcription factor 2 (Runx2), Msh homeobox 2 (Msx2), osteopontin (OPN) and osteocalcin (OCN), and the activity of alkaline phosphatase (ALP), as well as the calcified area observed by Alizarin red‑S staining, were investigated. Chromatin immunoprecipitation (ChIP) assay was used to detect the effect of 1,25(OH)2D3 on the DNA methylation and histone modification of BMP2, while an immunoprecipitation (IP) assay was performed to assess the crosstalk between Smad1 and disheveled‑1 (Dvl‑1) proteins. It was observed that 1,25(OH)2D3 significantly decreased the expression levels of BMP2, Runx2, Msx2, OPN and OCN, and reduced ALP activity and the calcified area in BMSCs, whereas these effects were rescued by BMP2 overexpression. ChIP assay revealed that BMSCs treated with 1,25(OH)2D3 exhibited a significant increase in H3K9me2 level and a decrease in the acetylation of histone H3 at the same BMP2 promoter region. In addition, 1,25(OH)2D3 treatment promoted the nuclear accumulation of β‑catenin by downregulating BMP2. Furthermore, the β‑catenin signaling inhibitor XAV‑939 weakened the inhibitory effect of 1,25(OH)2D3 on osteogenic differentiation. Additionally, knockdown of β‑catenin rescued the attenuation in Dvl‑1 and Smad1 interaction caused by 1,25(OH)2D3. Overexpression of Smad1 also reversed the inhibitory effect of 1,25(OH)2D3 on osteogenic differentiation. Taken together, the current study demonstrated that 1,25(OH)2D3 inhibited the differentiation of BMSCs into osteoblast‑like cells by inactivating BMP2 and activating Wnt/β‑catenin signaling.
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http://dx.doi.org/10.3892/mmr.2020.11619DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7646955PMC
December 2020

Revealing the subtyping of non-small cell lung cancer based on genomic evolutionary patterns by multi-region sequencing.

Cancer Med 2020 12 20;9(24):9485-9498. Epub 2020 Oct 20.

College of Bioinformatics Science and Technology, Harbin Medical University, Harbin, Heilongjiang, China.

Accurately classifying patients with non-small cell lung cancer (NSCLC) from the perspective of tumor evolution has not been systematically studied to date. Here, we reconstructed phylogenetic relationships of somatic mutations in 100 early NSCLC patients (327 lesions) through reanalyzing the TRACERx data. Based on the genomic evolutionary patterns presented on the phylogenetic trees, we grouped NSCLC patients into three evolutionary subtypes. The phylogenetic trees among three subtypes exhibited distinct branching structures, with one subtype representing branched evolution and another reflecting the early accumulation of genomic variation. However, in the evolutionary pattern of the third subtype, some mutations experienced selective sweeps and were gradually replaced by multiple newly formed subclonal populations. The subtype patients with poor prognosis had higher intra-tumor heterogeneity and subclonal diversity. We combined genomic heterogeneity with clinical phenotypes analysis and found that subclonal expansion results in the progression and deterioration of the tumor. The molecular mechanisms of subtype-specific Early Driver Feature (EDF) genes differed across the evolutionary subtypes, reflecting the characteristics of the subtype itself. In summary, our study provided new insights on the stratification of NSCLC patients based on genomic evolution that can be valuable for us to understand the development of pulmonary tumor profoundly.
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http://dx.doi.org/10.1002/cam4.3541DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774747PMC
December 2020

Coronavirus disease-19 vaccine development utilizing promising technology.

Curr Opin HIV AIDS 2020 11;15(6):351-358

Department of Pathogen Biology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin.

Purpose Of Review: Coronavirus disease-19 (COVID-19) is a highly transmittable and pathogenic pneumonia-causing disease, which is caused by severe acute respiratory syndrome coronavirus-2, resulting in millions of deaths globally. Severe acute respiratory syndrome coronavirus-2 may coexist with human populations for a long time. Therefore, high-effective COVID-19 vaccines are an urgent need.

Recent Findings: Vaccines help in the development of long-lasting humoral or cellular immunity, or both, by exposing individuals to antigens that induce an immunological response and memory prior to infections with live pathogens. New vaccine technologies, such as viral vectors and nucleic acid-based vaccines, which represent highly versatile technologies, may allow for faster vaccine manufacture and scale up production.

Summary: We summarized the recent progress made in relation to COVID-19 vaccine development using several promising technologies, with particular emphasis on advancements that are currently at the clinical trial stage.
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http://dx.doi.org/10.1097/COH.0000000000000648DOI Listing
November 2020

Prediction of transcription factors binding events based on epigenetic modifications in different human cells.

Epigenomics 2020 08 14;12(16):1443-1456. Epub 2020 Sep 14.

School of Life Science & Technology, Computational Biology Research Center, Harbin Institute of Technology, Harbin 150001, China.

We aim to predict transcription factor (TF) binding events from knowledge of gene expression and epigenetic modifications. TF-binding events based on the Encode project and The Cancer Genome Atlas data were analyzed by the random forest method. We showed the high performance of TF-binding predictive models in GM12878, HeLa, HepG2 and K562 cell lines and applied them to other cell lines and tissues. The genes bound by the top TFs ( and ) were significantly associated with cancer-related processes such as cell proliferation and DNA repair. We successfully constructed TF-binding predictive models in cell lines and applied them in tissues.
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http://dx.doi.org/10.2217/epi-2019-0321DOI Listing
August 2020

The stability of four organophosphorus insecticides in stored cucumber samples is affected by additives.

Food Chem 2020 Nov 15;331:127352. Epub 2020 Jun 15.

College of Science, China Agricultural University, Beijing 100193, China.

The influence of some additives, including metal ions, antioxidants, enzyme inhibitors and organic solvents, on the storage stability of four organophosphorus pesticides in cucumber samples were investigated. It was found that metal ions, including Al, Fe, and Co, increased the stability of dichlorvos, malathion, and chlorpyrifos. Conversely, Al, Fe, Fe, and Co caused catalytic degradation of diazinon. With the addition of organic solvents (CHCl, CHCl, CCl, CHOH and CHCOCH), remaining of diazinon residues was higher (16-54%) after storage for seven days. CCl was associated with the highest retention of malathion, diazinon, and chlorpyrifos (33%, 48% and 44%, respectively) in samples. SDS also stabilized the pesticides since residues were, again, higher (13-38%) after seven days storage. Furthermore, addition of Al and Fe decreased peroxidase (POD) activity and inhibited degradation of dichlorvos and malathion. After 14 days, lyophilization increased the pesticide residues remaining by 36%, 29%, and 58% for diazinon, malathion and chlorpyrifos, respectively. Overall, the stability of these pesticides during storage is impacted by water content and addition of exogenous substances. This could ensure higher quality of pesticide residue data in samples.
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http://dx.doi.org/10.1016/j.foodchem.2020.127352DOI Listing
November 2020

Biosensors Based on Advanced Sulfur-Containing Nanomaterials.

Sensors (Basel) 2020 Jun 19;20(12). Epub 2020 Jun 19.

State Key Laboratory of Bioelectronics, National Demonstration Center for Experimental Biomedical Engineering Education, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.

In recent years, sulfur-containing nanomaterials and their derivatives/composites have attracted much attention because of their important role in the field of biosensor, biolabeling, drug delivery and diagnostic imaging technology, which inspires us to compile this review. To focus on the relationships between advanced biomaterials and biosensors, this review describes the applications of various types of sulfur-containing nanomaterials in biosensors. We bring two types of sulfur-containing nanomaterials including metallic sulfide nanomaterials and sulfur-containing quantum dots, to discuss and summarize the possibility and application as biosensors based on the sulfur-containing nanomaterials. Finally, future perspective and challenges of biosensors based on sulfur-containing nanomaterials are briefly rendered.
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http://dx.doi.org/10.3390/s20123488DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7349518PMC
June 2020

TRIM35 mediates protection against influenza infection by activating TRAF3 and degrading viral PB2.

Protein Cell 2020 12 19;11(12):894-914. Epub 2020 Jun 19.

State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China.

Tripartite motif (TRIM) family proteins are important effectors of innate immunity against viral infections. Here we identified TRIM35 as a regulator of TRAF3 activation. Deficiency in or inhibition of TRIM35 suppressed the production of type I interferon (IFN) in response to viral infection. Trim35-deficient mice were more susceptible to influenza A virus (IAV) infection than were wild-type mice. TRIM35 promoted the RIG-I-mediated signaling by catalyzing Lys63-linked polyubiquitination of TRAF3 and the subsequent formation of a signaling complex with VISA and TBK1. IAV PB2 polymerase countered the innate antiviral immune response by impeding the Lys63-linked polyubiquitination and activation of TRAF3. TRIM35 mediated Lys48-linked polyubiquitination and proteasomal degradation of IAV PB2, thereby antagonizing its suppression of TRAF3 activation. Our in vitro and in vivo findings thus reveal novel roles of TRIM35, through catalyzing Lys63- or Lys48-linked polyubiquitination, in RIG-I antiviral immunity and mechanism of defense against IAV infection.
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http://dx.doi.org/10.1007/s13238-020-00734-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7719147PMC
December 2020

Full-frequency correction of spatial impulse response in back-projection scheme using space-variant filtering for optoacoustic mesoscopy.

Photoacoustics 2020 Sep 21;19:100193. Epub 2020 May 21.

Institute for Biological and Medical Imaging, Technical University of Munich and Helmholtz Center Munich, Neuherberg, 85764, Germany.

The fidelity and quality of reconstructed images in optoacoustic mesoscopy (OPAM) can be significantly improved by considering the spatial impulse response (SIR) of the employed focused transducer into reconstruction. However, the traditional method fully taking the SIR into account can hardly meet the data-intensive requirements of high resolution OPAM because of excessive memory and time consumption. Herein, a modified back-projection method using a space-variant filter for full-frequency correction of the SIR is presented, and applied to the OPAM system with a sphere-focused transducer. The proposed method can readily manage the large datasets of the OPAM and effectively reduce the extra time consumption. The performance of the proposed method is showcased by simulations and experiments of phantoms and biological tissue. The results demonstrate that the modified back-projection method exhibits better image fidelity, resolution and contrast compared to the common and weighted back-projection methods that are not or not fully accounting for the SIR.
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http://dx.doi.org/10.1016/j.pacs.2020.100193DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7264078PMC
September 2020

A novel turn-on fluorescent sensor for the sensitive detection of glutathione via gold nanocluster preparation based on controllable ligand-induced etching.

Analyst 2020 Jun;145(12):4265-4275

State Key Laboratory of Bioelectronics, National Demonstration Center for Experimental Biomedical Engineering Education, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.

In this study, we report a facile one-pot chemical etching approach to simply and rapidly prepare gold nanoclusters capped with luminol (Lum-AuNCs) in an alkaline aqueous solution at room temperature. A series of characterization studies have been carried out to explore the morphology, the optical properties and chemical components of Lum-AuNCs. The average diameter of Lum-AuNCs is 1.8 ± 0.3 nm, exhibiting fluorescence near 510 nm upon excitation at 420 nm with a quantum yield of 14.29% and an average fluorescence lifetime of 9.47 ns. On the basis of the ligand-induced etching of glutathione (GSH) to the intermediate (luminol capped gold nanoparticles, abbreviated as Lum-AuNPs), a novel and simple method for the fluorescence determination of GSH has been established. The method displays a good linear response in the range of 0.05-300 μM toward GSH with a limit of detection of 35 nM. This detection strategy with high sensitivity and selectivity facilitates its practical application for the detection of GSH levels in cell extracts. The in vitro cell results illustrate that Lum-AuNCs have good cytocompatibility and can be used to readily differentiate normal cells and tumor cells.
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http://dx.doi.org/10.1039/d0an00807aDOI Listing
June 2020

The city-level precision industrial emission reduction management based on enterprise performance evaluation and path design: A case of Changzhi, China.

Sci Total Environ 2020 Sep 12;734:139350. Epub 2020 May 12.

Beijing Mihot Huanxin Big-Data Technology Co., Ltd, Beijing 100022, China. Electronic address:

Realizing precision management, which is of great importance in city-level emission reduction management, requires scientific identification of key enterprises and differentiated emission reduction measures. However, current studies have not considered the enterprises units, or have not proposed the emission reduction paths of them. To solve this problem, this study chooses Changzhi, an industrialized city in China as a case, and considers 54 enterprises from thermal-power, cement, coking, and iron and steel sectors. The pollution performances, including the indicators of energy intensity and emission intensities of SO, NO, and PM of the 54 enterprises are evaluated. After identifying the key enterprises, this study designs their emission reduction paths including three types of measures, and quantifies the emission reduction potential. The results show that: (1) The 54 enterprises have imbalanced pollution performances, as the values have difference of 2-4 orders of magnitude. 13, 10, and 19 enterprises are classified into level A, B, and C respectively. (2) The emission reduction paths of 24 key enterprises are designed, which can reduce 3441.21, 4507.85, and 1683.12 tons of SO, NO, and PM. This accounts for 29.4%, 21.2%, and 14.9% of the total emissions. Based on these results, this study puts forward some policy suggestions of precision management measures in Changzhi. In sum, this study provides a methodology into quantitative analysis of precision air pollutant emission reduction management at city level, and put forward some critical insights of cleaner and sustainable production of the enterprises.
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http://dx.doi.org/10.1016/j.scitotenv.2020.139350DOI Listing
September 2020