Publications by authors named "Yifeng Wang"

304 Publications

Mutations in RNA Methyltransferase Gene Confer High Risk of Outflow Tract Malformation.

Front Cell Dev Biol 2021 21;9:623394. Epub 2021 Apr 21.

State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China.

, encoding a cytosine-5 RNA methyltransferase and located in the 7q11.23 locus, is a candidate gene for tetralogy of Fallot (TOF). Deletion of the 7q11.23 locus in humans is linked to cardiac outflow tract (OFT) disorders including TOF. We identified four potential pathogenic mutations in the coding region of and which were enriched in TOF patients by an association study of 132 TOF patients and 2,000 in-house controls ( = 1.44 × 10). We then generated a null () mouse model to validate the human findings by defining the functions of in OFT morphogenesis. The OFT did not develop properly in the deletion embryonic heart. We found a misalignment of the aorta and septum defects caused by the delayed fusion of the membraneous ventricular spetum as an OFT development delay. This caused OFT development delay in 27 of 64 (42.2%) mice. Moreover, we also found OFT development delay in 8 of 51 (15.7%) mice. Further functional experiments showed that the loss of function impaired the 5-methylcytosine (mC) modification and translation efficiency of essential cardiac genes. is required for normal OFT morphogenesis and it regulates the mC modification of essential cardiac genes. Our findings suggest the involvement of in the pathogenesis of TOF.
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http://dx.doi.org/10.3389/fcell.2021.623394DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097101PMC
April 2021

Global Signal Topography of the Human Brain: A Novel Framework of Functional Connectivity for Psychological and Pathological Investigations.

Front Hum Neurosci 2021 25;15:644892. Epub 2021 Mar 25.

Institute of Brain and Psychological Sciences, Sichuan Normal University, Chengdu, China.

The global signal (GS), which was once regarded as a nuisance of functional magnetic resonance imaging, has been proven to convey valuable neural information. This raised the following question: what is a GS represented in local brain regions? In order to answer this question, the GS topography was developed to measure the correlation between global and local signals. It was observed that the GS topography has an intrinsic structure characterized by higher GS correlation in sensory cortices and lower GS correlation in higher-order cortices. The GS topography could be modulated by individual factors, attention-demanding tasks, and conscious states. Furthermore, abnormal GS topography has been uncovered in patients with schizophrenia, major depressive disorder, bipolar disorder, and epilepsy. These findings provide a novel insight into understanding how the GS and local brain signals coactivate to organize information in the human brain under various brain states. Future directions were further discussed, including the local-global confusion embedded in the GS correlation, the integration of spatial information conveyed by the GS, and temporal information recruited by the connection analysis. Overall, a unified psychopathological framework is needed for understanding the GS topography.
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http://dx.doi.org/10.3389/fnhum.2021.644892DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8026854PMC
March 2021

Tumor Immunometabolism Characterization in Ovarian Cancer With Prognostic and Therapeutic Implications.

Front Oncol 2021 16;11:622752. Epub 2021 Mar 16.

Department of Gynecology, Obstetrics and Gynecology Center, Zhujiang Hospital, Southern Medical University, Guangzhou, China.

Metabolic dysregulation in the tumor microenvironment has significant impact on immune infiltration and immune responses. However, interaction between immunity and metabolism in the ovarian microenvironment requires further exploration. We constructed an immunometabolism gene set and ovarian cancer cohort from The Cancer Genome Atlas (TCGA) and classified these into three immunometabolism subtypes. We explored the relationships between immune infiltration and metabolic reprogramming. Additionally, we built risk score and nomogram as prognostic signatures. Three distinctive immunometabolism subtypes were identified with therapeutic and prognostic implications. Subtype 1, the "immune suppressive-glycan metabolism subtype," featured high levels of immunosuppressive cell infiltration and glycan metabolism activation; Subtype 2, the "immune inflamed-amino acid metabolism subtype," showed abundant adaptive immune cell infiltration and amino acid metabolism activation; Subtype 3, the "immune desert-endocrine subtype," was characterized by low immune cell infiltration and upregulation of hormone biosynthesis. Furthermore, we found that epinephrine biosynthesis displayed a significantly negative correlation with MHC molecules, which may result in defective antigen presentation. We proposed immunometabolism subtypes with prognostic implications and provided new perspectives for the ovarian cancer microenvironment.
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http://dx.doi.org/10.3389/fonc.2021.622752DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8008085PMC
March 2021

Temperature-Sensitive Lipid-Coated Carbon Nanotubes for Synergistic Photothermal Therapy and Gene Therapy.

ACS Nano 2021 04 22;15(4):6517-6529. Epub 2021 Mar 22.

Key Laboratory of Biotechnology and Bioresources Utilization of Ministry of Education, Dalian Minzu University, Dalian 116600, China.

The combination of photothermal therapy (PTT) and gene therapy (GT) shows great potential to achieve synergistic anti-tumor activity. However, the lack of a controlled release of genes from carriers remains a severe hindrance. Herein, peptide lipid (PL) and sucrose laurate (SL) were used to coat single-walled carbon nanotubes (SCNTs) and multi-walled carbon nanotubes (MCNTs) to form bifunctional delivery systems (denoted SCNT-PS and MCNT-PS, respectively) with excellent temperature-sensitivity and photothermal performance. CNT/siRNA suppressed tumor growth by silencing survivin expression while exhibiting photothermal effects under near-infrared (NIR) light. SCNT-PS/siRNA showed very high anti-tumor activity, resulting in the complete inhibition of some tumors. It was highly efficient for systemic delivery to tumor sites and to facilitate siRNA release owing to the phase transition of the temperature-sensitive lipids, due to PL and SL coating. Thus, SCNT-PS/siRNA is a promising anti-tumor nanocarrier for combined PTT and GT.
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http://dx.doi.org/10.1021/acsnano.0c08790DOI Listing
April 2021

Safety and feasibility of single-incision radical vulvectomy: a novel approach for the treatment of vulvar cancer.

Ann Transl Med 2021 Feb;9(4):320

Department of Gynecology, Obstetrics and Gynecology Center, Zhujiang Hospital, Southern Medical University, Guangzhou, China.

Background: In the process of decreasing the morbidity of wound-related complications after vulvectomy and IL for treating vulvar malignancy, we performed a novel surgical procedure-single-incision radical vulvectomy (SIRV). Here, we share our initial experience and report its safety and feasibility.

Methods: Patients with advanced local vulvar tumors were sequentially enrolled in this prospective cohort study to undergo SIRV. While performing SIRV, routine radical vulvectomies were performed first. Subsequently, the flaps of the bridge area between the vulvectomy incisions and femoral triangles were separated and the lymph nodes underneath were removed. Anterior working spaces (AWS) before the femoral triangle were then made. The saphenous vein was carefully identified and retained, while the superficial and deep inguinal lymph nodes were removed from the medial to the lateral sides. After careful hemostasis, the wounds were sutured. Patient demographics, clinical data, pathologic data, operation time, node count, and complications were recorded.

Results: Ten patients underwent SIRV for vulvar cancer. Average hospital stay was 11.70±3.16 (range, 9-13) days. The average number of harvested lymph nodes was 7.59±3.62 (range, 3-15) and 15.14±3.63 (range, 11-20) for per side or both sides of the groin. Blood loss was ≤35 mL. Three patients developed inguinal lymphoceles and underwent needle aspirations. Two patients had impaired wound healing and achieved healing after dressing change. No other postoperative complications were noted during follow-up.

Conclusions: Compared with conventional open inguinal lymphadenectomy (COIL) and video endoscopic inguinal lymphadenectomy (VEIL), SIRV is a more minimally invasive procedure. Our short-term observations showed that SIRV is safe and feasible and has good future application prospects for vulvar cancer. However, definitive conclusions cannot be made. Therefore, long-term oncologic outcomes and large-scale clinical trials are warranted.
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http://dx.doi.org/10.21037/atm-20-6077DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7944291PMC
February 2021

Intestinal fatty acid-binding protein, a biomarker of intestinal barrier dysfunction, increases with the progression of type 2 diabetes.

PeerJ 2021 2;9:e10800. Epub 2021 Feb 2.

Department of Critical Care Medicine, Taizhou Central Hospital (Taizhou University Hospital), Taizhou, China.

Objective: To investigate serum intestinal fatty acid-binding protein (I-FABP) in two groups of patients with different duration of hyperglycemia in a cross-sectional study.

Materials And Methods: In the present study, a total of 280 individuals (158 outpatients and 122 inpatients) suffering from hyperglycemia were recruited between May and September 2019. The clinical information of all participants was collected from the hospital information system, including the duration of hyperglycemia, age, gender, hemoglobin A1c (HbA1c), 75-g oral glucose tolerance test including fasting plasma glucose (FPG), 2-hour plasma glucose (2hPG), fasting C-peptide (FC-pep), 2-hour C-peptide (2hC-pep), fasting insulin (FIns), and 2-hour insulin (2hIns). In addition, the morbidity of diabetic complications (retinopathy, neuropathy, and nephropathy) in the inpatient group was determined. Furthermore, the difference between 2hPG and FPG (ΔPG), the difference between 2hC-pep and FC-pep (ΔC-pep), and the difference between 2hIns and FIns (ΔIns) were calculated. The level of serum I-FABP, a biomarker of intestinal barrier (IB) dysfunction, was estimated by an enzyme-linked immunosorbent assay.

Results: For the outpatient group, the median duration of hyperglycemia was less than a year; the serum I-FABP level was positively correlated with age ( = 0.299,  < 0.001). For the inpatient group, the median duration of hyperglycemia was ten years; correlation analysis showed that the serum I-FABP level was positively associated with age and ΔPG ( = 0.286,  = 0.001;  = 0.250,  = 0.006, respectively) while negatively associated with FC-pep and 2hC-pep ( =  - 0.304,  = 0.001;  =  - 0.241,  = 0.008, respectively); multiple linear regression analysis showed that the serum I-FABP level was positively associated with the duration of hyperglycemia (β = 0.362,  < 0.001); moreover, patients with retinopathy had a significantly higher I-FABP level than those without retinopathy ( = 0.001).

Conclusions: In the outpatients whose duration of hyperglycemia was less than a year, the serum I-FABP level was positively associated with age. In the inpatients with different courses of diabetes, the serum I-FABP level was positively associated with the duration of hyperglycemia and glycemic variability but negatively associated with islet beta-cell function; moreover, the serum I-FABP level was higher in patients with retinopathy than in those without retinopathy, suggesting that the IB dysfunction got worse with the progression of diabetes.
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http://dx.doi.org/10.7717/peerj.10800DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7863777PMC
February 2021

MELK Inhibition Effectively Suppresses Growth of Glioblastoma and Cancer Stem-Like Cells by Blocking AKT and FOXM1 Pathways.

Front Oncol 2020 14;10:608082. Epub 2021 Jan 14.

Institute of Nervous System Diseases, Xuzhou Medical University, Xuzhou, China.

Glioblastoma multiforme (GBM) is a devastating disease yet no effective drug treatment has been established to date. Glioblastoma stem-like cells (GSCs) are insensitive to treatment and may be one of the reasons for the relapse of GBM. Maternal embryonic leucine zipper kinase gene () plays an important role in the malignant proliferation and the maintenance of GSC stemness properties of GBM. However, the therapeutic effect of targeted inhibition of on GBM remains unclear. This study analyzed the effect of a MELK oral inhibitor, OTSSP167, on GBM proliferation and the maintenance of GSC stemness. OTSSP167 significantly inhibited cell proliferation, colony formation, invasion, and migration of GBM. OTSSP167 treatment reduced the expression of cell cycle G2/M phase-related proteins, Cyclin B1 and Cdc2, while up-regulation the expression of p21 and subsequently induced cell cycle arrest at the G2/M phase. OTSSP167 effectively prolonged the survival of tumor-bearing mice and inhibited tumor cell growth in mouse models. It also reduced protein kinase B (AKT) phosphorylation levels by OTSSP167 treatment, thereby disrupting the proliferation and invasion of GBM cells. Furthermore, OTSSP167 inhibited the proliferation, neurosphere formation and self-renewal capacity of GSCs by reducing forkhead box M1 (FOXM1) phosphorylation and transcriptional activity. Interestingly, the inhibitory effect of OTSSP167 on the proliferation of GSCs was 4-fold more effective than GBM cells. In conclusion, MELK inhibition suppresses the growth of GBM and GSCs by double-blocking AKT and FOXM1 signals. Targeted inhibition of MELK may thus be potentially used as a novel treatment for GBM.
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http://dx.doi.org/10.3389/fonc.2020.608082DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7842085PMC
January 2021

[Pregnancy-preserving and maternal-fetal management in a patient with rare large cell neuroendocrine carcinoma of the uterine cervix].

Nan Fang Yi Ke Da Xue Xue Bao 2021 Jan;41(1):1-9

Department of Obstetrics and Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China.

Objective: To explore the strategy of pregnancy-preserving and maternal- fetal management in patients with primary gynecologic neuroendocrine tumors (gNETs) during pregnancy.

Methods: We performed whole genome sequencing (WGS) for analyzing maternal and fetal somatic and germline single nucleotide variations (SNVs) and small insertions and deletions (InDels) for a 29-year-old pregnant woman diagnosed with stage IB2 large cell neuroendocrine carcinoma (LCNEC) and adenocarcinoma in the cervix. A systematic literature review was performed to explore the strategies for treatment of such rare histological type while maintaining pregnancy.

Results: Global case analysis of cervical NETs during pregnancy suggested that negative lymph node metastasis and an early FIGO stage were potentially associated with a good prognosis of the patients. In the case presented herein, a pregnancy-preserving strategy was adopted and favorable maternal-fetal outcomes were achieved after neoadjuvant chemotherapy, radical surgery and postoperative systemic chemotherapy. At 35 weeks, the fetus was delivered by caesarian section, and the patient has by now had a disease-free survival of 19 months postoperatively. WGS analysis revealed 6 missense somatic pathogenic mutations in two cancer tissues of the patient, and among them KARS and VEGFA were related with targeted therapy. Five pathogenic germline variants were detected in the patient and her son, suggesting the necessity of a long-term follow-up schedule including precise genetic counselling for both the mother and the child.

Conclusions: Although gNETs in pregnancy are rare and highly risky, pregnancy-preserving managements of gNETs can still be considered and favorable maternalfetal outcomes are possible with proper assessment of the clinical indications and implementation of multimodal treatments. Precise treatment and follow-up strategies based on the results of WGS for risk-reducing intervention of cancer recurrence or occurrence can potentially benefit the patient and the neonate.
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http://dx.doi.org/10.12122/j.issn.1673-4254.2021.01.01DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7867493PMC
January 2021

Understanding controls on the geochemistry of hydrocarbon produced waters from different basins across the US.

Environ Sci Process Impacts 2021 Feb;23(1):28-47

National Energy Technology Laboratory, US Department of Energy, Pittsburgh, PA 15236, USA.

The most massive waste stream generated by conventional and unconventional hydrocarbon exploration is the produced water (PW). The costs and environmental issues associated with the management and disposal of PW, which contains high concentrations of inorganic and organic pollutants, is one of the most challenging problems faced by the oil and gas industry. Many of the current strategies for the reuse and recycling of PW are inefficient because of varying water demand and the spatial and temporal variations in the chemical composition of PW. The chemical composition of PW is controlled by a multitude of factors and can vary significantly over time. This study aims to understand different parameters and processes that control the quality of PW generated from hydrocarbon-bearing formations by analyzing relationships between their major ion concentrations, O, H, and Sr isotopic composition. We selected PW data sets from three conventional (Trenton, Edwards, and Wilcox Formations) and four unconventional (Lance, Marcellus, Bakken, and Mesaverde Formations) oil and gas formations with varying lithology and depositional environment. Using comparative geochemical data analysis, we determined that the geochemical signature of PW is controlled by a complex interplay of several factors, including the original source of water (connate marine vs. non-marine), migration of the basinal fluids, the nature and degree of water-mineral-hydrocarbon interactions, water recharge, processes such as evaporation and ultrafiltration, and production techniques (conventional vs. unconventional). The development of efficient PW recycle and reuse strategies requires a holistic understanding of the geological and hydrological history of each formation to account for the temporal and spatial heterogeneities.
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http://dx.doi.org/10.1039/d0em00388cDOI Listing
February 2021

Biallelic mutations of CFAP58 are associated with multiple morphological abnormalities of the sperm flagella.

Clin Genet 2021 Mar 18;99(3):443-448. Epub 2020 Dec 18.

Department of Andrology, First Affiliated Hospital of Fujian Medical University, Fuzhou, China.

Multiple morphological abnormalities of the sperm flagella (MMAF) is a severe type of teratozoospermia. In this study, whole-exome sequencing was conducted on 55 patients with MMAF, and biallelic mutations of CFAP58 were identified in two patients. The variants are rare and pathogenic, and CFAP58 was absent in the CFAP58-mutated sperm. The F037/II:1 couple benefited from intracytoplasmic sperm injection (ICSI). This study further indicated that CFAP58 is a pathogenic gene associated with MMAF and ICSI is an effective treatment.
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http://dx.doi.org/10.1111/cge.13898DOI Listing
March 2021

Frequency-specific altered global signal topography in drug-naïve first-episode patients with adolescent-onset schizophrenia.

Brain Imaging Behav 2020 Nov 13. Epub 2020 Nov 13.

The Clinical Hospital of Chengdu Brain Science Institute, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, People's Republic of China.

Adolescent-onset schizophrenia (AOS) is a severe neuropsychiatric disease associated with frequency-specific abnormalities across distributed neural systems in a slow rhythm. Recently, functional magnetic resonance imaging (fMRI) studies have determined that the global signal. (GS) is an important source of the local neuronal activity in 0.01-0.1 Hz frequency band. However, it remains unknown whether the effects follow a specific spatially preferential pattern in different frequency bands in schizophrenia. To address this issue, resting-state fMRI data from 39 drug-naïve AOS patients and 31 healthy controls (HCs) were used to assess the changes in GS topography patterns in the slow-4 (0.027-0.073 Hz) and slow-5 bands (0.01-0.027 Hz). Results revealed that GS mainly affects the default mode network (DMN) in slow-4 and sensory regions in the slow-5 band respectively, and GS has a stronger driving effect in the slow-5 band. Moreover, significant frequency-by-group interaction was observed in the frontoparietal network. Compared with HCs, patients with AOS exhibited altered GS topography mainly located in the DMN. Our findings demonstrated that the influence of the GS on brain networks altered in a frequency-specific way in schizophrenia.
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http://dx.doi.org/10.1007/s11682-020-00381-9DOI Listing
November 2020

Spatial variability of low frequency brain signal differentiates brain states.

PLoS One 2020 12;15(11):e0242330. Epub 2020 Nov 12.

The Clinical Hospital of Chengdu Brain Science Institute, MOE Key Lab for Neuroinformation, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.

Temporal variability of the neural signal has been demonstrated to be closely related to healthy brain function. Meanwhile, the evolving brain functions are supported by dynamic relationships among brain regions. We hypothesized that the spatial variability of brain signal might provide important information about brain function. Here we used the spatial sample entropy (SSE) to investigate the spatial variability of neuroimaging signal during a steady-state presented face detection task. Lower SSE was found during task state than during resting state, associating with more repetitive functional interactions between brain regions. The standard deviation (SD) of SSE during the task was negatively related to the SD of reaction time, suggesting that the spatial pattern of neural activity is reorganized according to particular cognitive function and supporting the previous theory that greater variability is associated with better task performance. These results were replicated with reordered data, implying the reliability of SSE in measuring the spatial organization of neural activity. Overall, the present study extends the research scope of brain signal variability from the temporal dimension to the spatial dimension, improving our understanding of the spatiotemporal characteristics of brain activities and the theory of brain signal variability.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0242330PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7660497PMC
January 2021

Death associated protein kinase 2 suppresses T-B interactions and GC formation.

Mol Immunol 2020 12 8;128:249-257. Epub 2020 Nov 8.

Tsinghua-Peking Center for Life Sciences, China; Laboratory of Dynamic Immunobiology, Institute for Immunology, China; Department of Basic Medical Sciences, School of Medicine, China, Tsinghua University, Beijing 100084, China; Beijing Key Laboratory for Immunological Research on Chronic Diseases, Tsinghua University, Beijing 100084, China; Beijing Frontier Research Center for Biological Structure, Tsinghua University, Beijing 100084, China. Electronic address:

Germinal center (GC) formation is a critical step during T-dependent humoral immune responses. We report Death Associated Protein Kinase 2, a serine/threonine kinase, is rapidly induced in T cells following activation and plays an inhibitory role in T cell-mediated help for the GC formation. Specifically, T cells deficient in Dapk2 have an increased ability to physically conjugate with antigen-presenting B cells and to promote GC formation. However, Dapk2 does not regulate T cell receptor signaling strength and does not influence cytokine-driven T-cell subset polarization. Instead, Dapk2 dampens mTORC1 activities by associating with Raptor. Silencing of Raptor rescues defects observed with the Dapk2 insufficiency. Our study thus identifies Dapk2 as a new kinase likely involved in negative regulation of contact-dependent help delivery to B cells and GC formation.
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http://dx.doi.org/10.1016/j.molimm.2020.10.018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7754787PMC
December 2020

Linoleic acid-modified liposomes for the removal of protein-bound toxins: An in vitro study.

Int J Artif Organs 2020 Nov 2:391398820968837. Epub 2020 Nov 2.

Division of Nephrology, Shanghai Ninth People's Hospital, School of Medicine, Shanghai JiaoTong University, Shanghai, China.

Introduction: Protein-bound uremic toxins (PBUTs) and liver failure-related cholestatic solutes are associated with adverse outcomes in patients with chronic kidney disease (CKD) and liver failure, respectively, and are not easily removed by traditional dialysis therapies. We constructed linoleic acid-modified liposomes (LA-liposomes) as indirect adsorbent in the dialysate, and evaluated their effects on the clearance of the representative PBUTs and cholestatic solutes.

Methods: The LA-liposomes were prepared by the thin-film hydration method. The binding rates of liposomes and protein-bound solutes were detected by the ultrafiltration column. The in vitro dialysis experiments were performed using both non-current and current devices to assay the clearing efficiency of the dialysate supported by LA-liposomes.

Results: The LA-liposomes exhibited good binding properties to the PBUTs, bilirubin and bile acids. The LA-liposome dialysate showed higher solute reduction rates of the representative PBUTs and cholestatic solutes than the traditional dialysate or dialysate supported by the unmodified plain liposomes. Also, albumin binding of the PBUTs was significantly inhibited by the addition of linoleic acid (LA), and the removal efficiency of PBUTs was greatly enhanced by the combination of indirect adsorbent LA-liposomes and LA as the competitive displacer.

Conclusion: LA-liposomes were efficient in the clearance of the representative PBUTs and liver failure-related solutes. Moreover, the combination of indirect adsorbent LA-liposomes and competitive displacer suggested a potential application for the extremely highly-bound solutes.
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http://dx.doi.org/10.1177/0391398820968837DOI Listing
November 2020

Frequency-dependent circuits anchored in the dorsal and ventral left anterior insula.

Sci Rep 2020 10 5;10(1):16394. Epub 2020 Oct 5.

The Clinical Hospital of Chengdu Brain Science Institute, MOE Key Lab for Neuroinformation, School of Life Science and Technology, University of Electronic Science and Technology of China, No. 2006, Xiyuan Ave, Chengdu, 611731, China.

The hub role of the right anterior insula (AI) has been emphasized in cognitive neurosciences and been demonstrated to be frequency-dependently organized. However, the functional organization of left AI (LAI) has not been systematically investigated. Here we used 100 unrelated datasets from the Human Connectome Project to study the frequency-dependent organization of LAI along slow 6 to slow 1 bands. The broadband functional connectivity of LAI was similar to previous findings. In slow 6-slow 3 bands, both dorsal and ventral seeds in LAI were correlated to the salience network (SN) and language network (LN) and anti-correlated to the default mode network (DMN). However, these seeds were only correlated to the LAI in slow 2-slow 1 bands. These findings indicate that broadband and narrow band functional connections reflect different functional organizations of the LAI. Furthermore, the dorsal seed had a stronger connection with the LN and anti-correlation with DMN while the ventral seed had a stronger connection within the SN in slow 6-slow 3 bands. In slow 2-slow 1 bands, both seeds had stronger connections with themselves. These observations indicate distinctive functional organizations for the two parts of LAI. Significant frequency effect and frequency by seed interaction were also found, suggesting different frequency characteristics of these two seeds. The functional integration and functional segregation of LDAI and LVAI were further supported by their cognitive associations. The frequency- and seed-dependent functional organizations of LAI may enlighten future clinical and cognitive investigations.
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http://dx.doi.org/10.1038/s41598-020-73192-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7536237PMC
October 2020

Supramolecular Chemistry of Titanium Oxide Clusters.

Chemistry 2021 Mar 14;27(13):4270-4282. Epub 2021 Jan 14.

School of Chemistry and Chemical Engineering, Shandong University, Shanda South Road 27, 250100, Jinan, P.R. China.

Titanium oxide clusters (TOCs) have been emerging as a new type of inorganic molecular entities of supramolecular chemistry. Herein, a perspective on the structures and functionalities of TOCs over the past three decades is given and the paramount roles of TOCs in serving supramolecular chemistry are demonstrated. Four types of supramolecular assemblies based on TOCs are reviewed, namely, TOC hosts for ion inclusion, mechanically interlocked molecular systems built from cyclic TOCs, reactivities of surface sites toward ligand exchange, and hierarchical structures of TOCs. The principles and advantages of TOCs toward each application are fully discussed, along with structural analyses. Following this path, more functional TOC-based supramolecular systems may be designed and synthesized in the future, which, in turn, will certainly enhance research into both supramolecular and coordination chemistry of titanium.
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http://dx.doi.org/10.1002/chem.202003378DOI Listing
March 2021

Whole-exome sequencing in patients with premature ovarian insufficiency: early detection and early intervention.

J Ovarian Res 2020 Sep 22;13(1):114. Epub 2020 Sep 22.

Department of Reproductive Medicine, Women and Children's Hospital, School of Medicine, Xiamen University, Xiamen, 361005, Fujian, China.

Background: The loss of ovarian function in women, referred to as premature ovarian insufficiency (POI), is associated with a series of concomitant diseases. POI is genetically heterogeneous, and in most cases, the etiology is unknown.

Methods: Whole-exome sequencing (WES) was performed on DNA samples obtained from patients with POI, and Sanger sequencing was used to validate the detected potentially pathogenic variants. An in silico analysis was carried out to predict the pathogenicity of the variants.

Results: We recruited 24 patients with POI and identified variants in POI-related genes in 14 patients, including bi-allelic mutations in DNAH6, HFM1, EIF2B2, BNC, and LRPPRC and heterozygous variants in BNC1, EIF2B4, FOXL2, MCM9, FANCA, ATM, EIF2B3, and GHR. No variants in the above genes were detected in the WES data obtained from 29 women in a control group without POI. Determining a clear genetic etiology could significantly increase patient compliance with appropriate intervention strategies.

Conclusions: Our study confirmed that POI is a genetically heterogeneous condition and that whole-exome sequencing is a powerful tool for determining its genetic etiology. The results of this study will aid researchers and clinicians in genetic counseling and suggests the potential of WES for the detection of POI and thus early interventions for patients with POI.
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http://dx.doi.org/10.1186/s13048-020-00716-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7510158PMC
September 2020

Identification of Recurrent Variants in and across Multiple Cancers in the Chinese Population.

Biomed Res Int 2020 15;2020:6739823. Epub 2020 Aug 15.

State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, Jiangsu 211100, China.

and as important DNA repair genes have been thoroughly investigated in abundant studies. The potential relationships of pathogenic variants between multicancers have been verified in Caucasians but few in Chinese. In this study, we performed a two-stage study to screen pathogenic variants or variants of uncertain significance (VUS) with 7580 cancer cases and 4874 cancer-free controls, consisting of a discovery stage with 70 familial breast cancer cases and a subsequent validation stage with 7510 cases (3217 breast cancer, 1133 cervical cancer, 2044 hepatocellular carcinoma, and 1116 colorectal cancer). 48 variants were obtained from 70 familial breast cancer cases after exon detection, and finally, 20 pathogenic variants or VUS were selected for subsequent validation. Four recurrent variants in sporadic cases ( c.4801A>T, c.3257del, c.440del, and c.7409dup) were identified and three of them were labeled Class 5 by ENIGMA. Two variants ( c.3257del and c.440del) were specific in breast cancer cases, while c.7409dup and c.4307T>C were detected in two hepatocellular carcinoma patients and the c.4801A>T variant in one cervical cancer patient, respectively. Moreover, c.3257del was the most frequent variant observed in Chinese sporadic breast cancer and showed increased proliferation of -overexpressing triple-negative breast cancer cell lines (MDA-MB-231) in vitro. In addition to the known founder deleterious mutations, our findings highlight that the recurrently pathogenic variants in breast cancer cases could be taken as candidate genetic screening loci for a more efficient genetic screening of the Chinese population.
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http://dx.doi.org/10.1155/2020/6739823DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7448236PMC
August 2020

Electronic and effective mass modulation in 2D BCN by strain engineering.

Nanotechnology 2020 Nov 18;31(45):455702. Epub 2020 Aug 18.

College of Materials Science and Engineering, Nanjing Tech University, Nanjing 211816, People's Republic of China.

2D BCN material consisting of graphene and hexagonal boron nitride (h-BN) has received extensive attention due to its abundant electronic properties and promising applications. The actual applications of 2D BCN require that there be precise control over its electronic properties. Using density functional theory calculations, we systematically investigate the electronic structure and effective mass of 2D BCN under biaxial strain. It is demonstrated that the band gap of zigzag BCNs decreases monotonously as the tensile strain increases. Moreover, the system exhibits a similar trend, regardless of the C/h-BN ratio. In sharp contrast, the band gap of armchair BCNs depends on the C/h-BN ratio. Specifically, the band gap of C(BN) decreases significantly, while the band gap of C(BN) and C(BN) initially remains almost unchanged and then increases with increasing biaxial strain in armchair BCNs. In addition, it is found that the effective masses of the electron and hole of BCNs can be effectively modulated by the biaxial strain. Our results suggest a new route to control the electronic properties of 2D BCN and may also facilitate the realization of electronic devices based on 2D BCN material.
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http://dx.doi.org/10.1088/1361-6528/abaa73DOI Listing
November 2020

Realization of an Ultrahigh Power Factor and Enhanced Thermoelectric Performance in TiS via Microstructural Texture Engineering.

ACS Appl Mater Interfaces 2020 Sep 1;12(37):41687-41695. Epub 2020 Sep 1.

College of Materials Science and Engineering, Nanjing Tech University, Nanjing 210009, China.

Layered 1T-type TiS powders were pretreated by an ethanol-based shear pulverization process, which showed outstanding effectiveness in reducing the average grain size and narrowing the size distribution while maintaining high crystallinity and plate-shaped morphology. The resulting bulk ceramics densified by spark plasma sintering possessed a highly (00)-oriented texture and pronounced anisotropy. They showed a noticeably increased σ and an unaffected in the in-plane direction due to the increased carrier mobility μ and the constant carrier concentration , which resulted in a significant enhancement of the in-plane power factor, optimally to an unprecedented high level of 1.6-1.8 mW m K in a range of 323-673 K. Meanwhile, the lattice thermal conductivity was reduced by approximately 20% due to the intensified grain boundary phonon scattering that overwhelmed the effect due to texturing. These effects not only demonstrated the powder shear pulverization pretreatment as a facial and reliable route toward a high-textured TiS but also enabled a remarkable increase of record for TiS-based thermoelectrics (TEs) to approximately 0.7 at 673 K, indicating clearly the significant effect of texture engineering on TE performance.
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http://dx.doi.org/10.1021/acsami.0c09592DOI Listing
September 2020

An FGF15/19-TFEB regulatory loop controls hepatic cholesterol and bile acid homeostasis.

Nat Commun 2020 07 17;11(1):3612. Epub 2020 Jul 17.

Harold Hamm Diabetes Center, Department of Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, 73104, USA.

Bile acid synthesis plays a key role in regulating whole body cholesterol homeostasis. Transcriptional factor EB (TFEB) is a nutrient and stress-sensing transcriptional factor that promotes lysosomal biogenesis. Here we report a role of TFEB in regulating hepatic bile acid synthesis. We show that TFEB induces cholesterol 7α-hydroxylase (CYP7A1) in human hepatocytes and mouse livers and prevents hepatic cholesterol accumulation and hypercholesterolemia in Western diet-fed mice. Furthermore, we find that cholesterol-induced lysosomal stress feed-forward activates TFEB via promoting TFEB nuclear translocation, while bile acid-induced fibroblast growth factor 19 (FGF19), acting via mTOR/ERK signaling and TFEB phosphorylation, feedback inhibits TFEB nuclear translocation in hepatocytes. Consistently, blocking intestinal bile acid uptake by an apical sodium-bile acid transporter (ASBT) inhibitor decreases ileal FGF15, enhances hepatic TFEB nuclear localization and improves cholesterol homeostasis in Western diet-fed mice. This study has identified a TFEB-mediated gut-liver signaling axis that regulates hepatic cholesterol and bile acid homeostasis.
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http://dx.doi.org/10.1038/s41467-020-17363-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7368063PMC
July 2020

Frequency-specific alteration of functional connectivity density in bipolar disorder depression.

Prog Neuropsychopharmacol Biol Psychiatry 2021 01 2;104:110026. Epub 2020 Jul 2.

The Clinical Hospital of Chengdu Brain Science Institute, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China; MOE Key Lab for Neuroinformation, University of Electronic Science and Technology of China, Chengdu, China; High-Field Magnetic Resonance Brain Imaging Key Laboratory of Sichuan Province, University of Electronic Science and Technology of China, Chengdu, China; Department of Radiology, First Affiliated Hospital to Army Medical University, Chongqing, China. Electronic address:

Functional dysconnectivity has been widely reported in bipolar disorder during depressive episodes (BDD). However, the frequency-specific alterations of functional connectivity (FC) in BDD remain poorly understood. To address this issue, the FC patterns across slow-5 (0.01-0.027 Hz) and slow-4 (0.027-0.073 Hz) bands were computed using resting-state functional magnetic resonance imaging data from 37 BDD patients and 56 healthy controls (HCs). Short-range (local) FC density (lfcd) and long-range FC density (lrfcd) were calculated, and two-way analysis of variance was performed to ascertain the main effect of diagnosis and interaction effects between diagnosis and frequency. The BDD patients showed increased lfcd in the midline cerebelum. Meanwhile, the BDD patients showed increased lrfcd in the left supplementary motor cortex and right striatum and decreased lrfcd in the bilateral inferior temporal gyrus and left angular gyrus (AG) compared with the HCs. A significant frequency-by-diagnosis interaction was observed. In the slow-4 band, the BDD patients showed increased lfcd in the left pre-/postcentral gyrus and left fusiform gyrus (FG) and increased lrfcd in the left lingual gyrus (LG). In the slow-5 band, the BDD patients showed decreased lrfcd in the left LG. Moreover, the increased lfcd in the left FG in the slow-4 band was correlated with clinical progression and decreased lrfcd in the left AG was correlated with depressive severity. These results suggest that the presence of aberrant communication in the default mode network, sensory network, and subcortical and limbic modulating regions (striatum and midline cerebelum), which may offer a new framework for the understanding of the pathophysiological mechanisms of BDD.
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http://dx.doi.org/10.1016/j.pnpbp.2020.110026DOI Listing
January 2021

[Research progress on intrinsic signaling pathways in axon regeneration].

Zhejiang Da Xue Xue Bao Yi Xue Ban 2020 May;49(1):82-89

NHC and CAMS Key Laboratory of Medical Neurobiology, Center for Neuroscience, Zhejiang University School of Medicine, Hangzhou 310058, China.

The intrinsic regrowth ability of injured neurons is essential for axon regeneration and functional recovery. Recently, numerous intrinsic pathways that regulate axon regeneration have been discovered, among which the mitogen-activated protein kinase (MAPK) pathway and the phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) pathway are arguably the best characterized examples. MAPK signaling pathway is involved in multiple processes including sensing injury signals, initiating and promoting axonal regrowth through regulating cytoskeleton dynamics and protein synthesis. The PI3K/Akt signaling pathway regulates axon regeneration mainly through gene transcription and translation. Combinatory manipulation of multiple regeneration-promoting signals can further improve the extend of axonal regrowth. This paper summarizes current progresses on axon regeneration studies in various organisms and discuss their potentials in promoting functional recovery .
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May 2020

Abscisic acid promotes jasmonic acid biosynthesis via a 'SAPK10-bZIP72-AOC' pathway to synergistically inhibit seed germination in rice (Oryza sativa).

New Phytol 2020 11 28;228(4):1336-1353. Epub 2020 Jul 28.

State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 311400, China.

Abscisic acid (ABA) and jasmonic acid (JA) both inhibit seed germination, but their interactions during this process remain elusive. Here, we report the identification of a 'SAPK10-bZIP72-AOC' pathway, through which ABA promotes JA biosynthesis to synergistically inhibit rice seed germination. Using biochemical interaction and phosphorylation assays, we show that SAPK10 exhibits autophosphorylation activity on the 177 serine, which enables it to phosphorylate bZIP72 majorly on 71 serine. The SAPK10-dependent phosphorylation enhances bZIP72 protein stability as well as the DNA-binding ability to the G-box cis-element of AOC promoter, thereby elevating the AOC transcription and the endogenous concentration of JA. Blocking of JA biosynthesis significantly alleviated the ABA sensitivity on seed germination, suggesting that ABA-imposed inhibition partially relied on the elevated concentration of JA. Our findings shed a novel insight into the molecular networks of ABA-JA synergistic interaction during rice seed germination.
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http://dx.doi.org/10.1111/nph.16774DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7689938PMC
November 2020

In situ generation of silver nanoparticles and nanocomposite films based on electrodeposition of carboxylated chitosan.

Carbohydr Polym 2020 Aug 23;242:116391. Epub 2020 May 23.

School of Materials Science and Engineering, Wuhan University of Technology, 122 Luoshi Road, Wuhan, 430070, China.

Herein, for the first time the electrodeposition of carboxylated chitosan is studied and utilized for the synthesis of silver nanoparticles (AgNPs) and generation of AgNPs/carboxylated chitosan nanocomposite films. Particularly, AgNPs are in situ synthesized on electrodes or substrates during the electrodeposition. Carboxylated chitosan not only acts as the green reducing agent and stabilizing agent for preparing AgNPs, but also serves as the main component in the electrodeposited nanocomposite film. The experimental results indicate that a smooth and homogeneous film is formed on the silver plate after electrodeposition, and the electrodeposited film can be detached from the silver plate as an independent film. The TEM observation and spectroscopic analysis results confirm the existence of AgNPs (the average size of 10 nm) in the nanocomposite film. The nanocomposite films with various shapes can be fabricated by the spatial selectivity of electrodeposition. In addition, the nanocomposite film containing AgNPs shows favorable antibacterial properties.
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http://dx.doi.org/10.1016/j.carbpol.2020.116391DOI Listing
August 2020

Effects of carbon sources on production and properties of curdlan using sp. DH-2.

Prep Biochem Biotechnol 2020 13;50(9):857-864. Epub 2020 Jun 13.

College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, China.

Curdlan has wide potential application in the food and biomedical fields due to its unique thermal gel and biological activity. This study investigated the effect of six sugars including glucose, fructose, lactose, maltose, sucrose and xylose as carbon sources on production and properties of curdlan using sp. DH-2. The maximum production (38.1 g/L and 37.4 g/L, respectively) and yield (0.58 g curdlan/g sucrose and 0.53 g curdlan/g maltose, respectively) of curdlan were achieved by sucrose and maltose, followed by glucose, fructose, lactose and xylose. Scanning electron micrographs showed that the surface of cells was smooth in strain growth phase, while cells were covered by curdlan matrix acted as a net in the curdlan synthesis phase. The highest glucosyltransferase activity (19.9 U/g biomass) corresponded to the maximum curdlan production using the sucrose medium. The molecular weight and gel strength of curdlan were influenced by the carbon sources. The curdlan from xylose medium resulted in a maximum molecular weight of 1.59 × 10 Da and the highest gel strength of 989.2 g/cm, while the curdlan from sucrose medium resulted in a lowest molecular weight of 1.10 × 10 Da and gel strength of 672.8 g/cm. The high molecular weight of curdlan had high gel strength.
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http://dx.doi.org/10.1080/10826068.2020.1777423DOI Listing
May 2021

Combined static and dynamic functional connectivity signatures differentiating bipolar depression from major depressive disorder.

Aust N Z J Psychiatry 2020 08 26;54(8):832-842. Epub 2020 May 26.

The Clinical Hospital of Chengdu Brain Science Institute, MOE Key Lab for Neuroinformation, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.

Objective: Bipolar disorder in the depressive phase (BDd) may be misdiagnosed as major depressive disorder (MDD), resulting in poor treatment outcomes. To identify biomarkers distinguishing BDd from MDD is of substantial clinical significance. This study aimed to characterize specific alterations in intrinsic functional connectivity (FC) patterns in BDd and MDD by combining whole-brain static and dynamic FC.

Methods: A total of 40 MDD and 38 BDd patients, and 50 age-, sex-, education-, and handedness-matched healthy controls (HCs) were included in this study. Static and dynamic FC strengths (FCSs) were analyzed using complete time-series correlations and sliding window correlations, respectively. One-way analysis of variance was performed to test group effects. The combined static and dynamic FCSs were then used to distinguish BDd from MDD and to predict clinical symptom severity.

Results: Compared with HCs, BDd patients showed lower static FCS in the medial orbitofrontal cortex and greater static FCS in the caudate, while MDD patients exhibited greater static FCS in the medial orbitofrontal cortex. BDd patients also demonstrated greater static and dynamic FCSs in the thalamus compared with both MDD patients and HCs, while MDD patients exhibited greater dynamic FCS in the precentral gyrus compared with both BDd patients and HCs. Combined static and dynamic FCSs yielded higher accuracy than either static or dynamic FCS analysis alone, and also predicted anhedonia severity in BDd patients and negative mood severity in MDD patients.

Conclusion: Altered FC within frontal-striatal-thalamic circuits of BDd patients and within the default mode network/sensorimotor network of MDD patients accurately distinguishes between these disorders. These unique FC patterns may serve as biomarkers for differential diagnosis and provide clues to the pathogenesis of mood disorders.
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http://dx.doi.org/10.1177/0004867420924089DOI Listing
August 2020

MALT1 is a potential therapeutic target in glioblastoma and plays a crucial role in EGFR-induced NF-κB activation.

J Cell Mol Med 2020 07 25;24(13):7550-7562. Epub 2020 May 25.

Insititute of Nervous System Diseases, Xuzhou Medical University, Xuzhou, China.

Glioblastoma multiforme (GBM) is the most common malignant tumour in the adult brain and hard to treat. Nuclear factor κB (NF-κB) signalling has a crucial role in the tumorigenesis of GBM. EGFR signalling is an important driver of NF-κB activation in GBM; however, the correlation between EGFR and the NF-κB pathway remains unclear. In this study, we investigated the role of mucosa-associated lymphoma antigen 1 (MALT1) in glioma progression and evaluated the anti-tumour activity and effectiveness of MI-2, a MALT1 inhibitor in a pre-clinical GBM model. We identified a paracaspase MALT1 that is involved in EGFR-induced NF-kB activation in GBM. MALT1 deficiency or inhibition significantly affected the proliferation, survival, migration and invasion of GBM cells both in vitro and in vivo. Moreover, MALT1 inhibition caused G1 cell cycle arrest by regulating multiple cell cycle-associated proteins. Mechanistically, MALTI inhibition blocks the degradation of IκBα and prevents the nuclear accumulation of the NF-κB p65 subunit in GBM cells. This study found that MALT1, a key signal transduction cascade, can mediate EGFR-induced NF-kB activation in GBM and may be potentially used as a novel therapeutic target for GBM.
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http://dx.doi.org/10.1111/jcmm.15383DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7339184PMC
July 2020

Comparative separation methods and biological characteristics of human placental and umbilical cord mesenchymal stem cells in serum-free culture conditions.

Stem Cell Res Ther 2020 05 19;11(1):183. Epub 2020 May 19.

Department of Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou, 510280, Guangdong Province, China.

Background: Mesenchymal stem cells (MSCs) are considered to be an effective tool for regenerative medicine with promising applications for clinical therapy. However, incongruent data has been reported partially owing to their functional heterogeneity. To provide sufficient and suitable clinical seed cells derived from the placenta for MSC therapy, we compared the various current isolation methods, as well as the biological characteristics, of different human placenta mesenchymal stem cells (hPMSCs).

Methods: We selected placentas from 35 informed donors and exploited three commonly used methods. MSCs were isolated from different parts of placental tissue including umbilical cord (UC), amniotic membrane (AM), chorionic membrane (CM), chorionic villi (CV), and deciduae (DC). The appropriate isolation methods for each type of hPMSCs were first assessed. The resulting five MSC types from the same individuals were identified based on their surface marker expression, proliferation capacity, transcriptome, differentiation, multipotency and karyotype.

Results: All three methods successfully isolated the five hPMSC types from placental tissues. However, the UC-MSCs were most effectively separated via the tissue explant method, while the enzymatic digestion method was found to be more suitable for separating CV-MSCs, owing to its higher output efficiency compared to the other methods. Alternatively, the perfusion method was complicated and exhibited the lowest efficiency for cell isolation and uniformity. Furthermore, we determined that UC-MSCs and CV-MSCs express a higher level of paracrine cytokines and display much stronger proliferative capacity as well as superior extraction efficiency. Finally, karyotype analysis revealed that DC-MSCs are derived from the mother, while the other cell types are derived from the fetus. Moreover, the different hPMSCs exhibited unique gene expression profiles, which may prove advantageous in treatment of a broad range of diseases.

Conclusions: hPMSCs from different sources are similar yet also unique. Our results describe the biological characteristics of five hPMSCs and provide insights to aide in the selection process of candidates for MSCs treatment. Overall, UC- and CV-MSCs appear to be ideal sources of primary MSCs for clinical treatment and future research.
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http://dx.doi.org/10.1186/s13287-020-01690-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7238656PMC
May 2020

Bio-chemo-electro-mechanical modelling of the rapid movement of Mimosa pudica.

Authors:
Yifeng Wang Hua Li

Bioelectrochemistry 2020 Aug 22;134:107533. Epub 2020 Apr 22.

School of Mechanical and Aerospace Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Republic of Singapore. Electronic address:

A remarkable feature of Mimosa pudica is its ability to deform in response to certain external stimuli. Here, a two-dimensional transient bio-chemo-electro-mechanical model of the rapid movement of the main pulvinus of Mimosa pudica is developed. Based on the laws of mass and momentum conservation, poroelasticity, and representative volume elements, a novel fluid pressure equation is proposed to characterize the cell elasticity. Experiments were conducted to measure the time and amplitude of the rapid movement. After examinations with the published experiments, it is confirmed that the model can predict well the ionic concentrations, petiole bending angle, and membrane potential. The simulation analysis of the biophysical properties provides insights to biomechanics: the hydrostatic pressure in the lowest extensor decreases from 0.35 to 0.05 MPa at t = 0.00 to 3.00 s; fluid pressure increases from 0.00 to 0.11 MPa at t = 0.00 to 0.14 s; and the peak bending angle increases from 57.0° to 70.9° when the reflection coefficient is assigned as 0.10 to 0.20 in the model. The results highlight the biochemical actuation mechanism of the Mimosa pudica movement, and they confirm the importance of ionic and water transports for causing changes in osmotic and hydrostatic pressures.
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http://dx.doi.org/10.1016/j.bioelechem.2020.107533DOI Listing
August 2020