Publications by authors named "Yifeng He"

37 Publications

Ophiocordyceps lanpingensis polysaccharides alleviate chronic kidney disease through MAPK/NF-κB pathway.

J Ethnopharmacol 2021 Aug 6;276:114189. Epub 2021 May 6.

Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China; Key Laboratory of Sustainable Utilization of Panax notoginseng Resources of Yunnan Province, Kunming 650500, China. Electronic address:

Ethnopharmacological Relevance: Ophiocordyceps lanpingensis (O. lanpingensis) is a traditional ethno-medicine distributed in Eastern Himalayas, which has been used by local minorities to prevent and treat urinary diseases for hundreds of years. However, the corresponding active components and related pharmacological mechanism of such medication are not clear yet.

Aims Of The Study: This study was performed to investigate the effects and potential mechanisms of O. lanpingensis polysaccharides (OLP) in the treatment of chronic kidney disease (CKD) based on our previous research results.

Materials And Methods: Methylation analysis was used to investigate the monosaccharide composition and glycosidic linkages in OLP. The animals were divided into the control group, CKD model group, losartan group and three different doses of OLP groups. The CKD mouse model was established by the adenine gavage. The histological changes of renal tissue were observed by Hematoxylin-eosin and Masson staining. Biochemical indicators, including blood urea nitrogen (BUN), serum creatinine (Scr), serum phosphorus (P), plasma calcium (Ca), reactive oxygen species (ROS), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) were measured to evaluate the alleviation of CKD by OLP. Moreover, the expression levels of a series of cytokines related to the inflammation, apoptosis and fibrosis were analyzed to explore the possible mechanisms of OLP to treat CKD.

Results: OLP is composed of three kinds of monosaccharides. There are eight kinds of glycosidic linkages in OLP, among which →4)-Glcp-(1→ is the main linkage. OLP could significantly attenuate CKD in mice and the tubulointerstitial damage was recovered to almost normal after the treatment of OLP. Compared with the CKD model group, the levels of Scr, BUN, MDA, P in OLP treatment groups were significantly decreased; and the levels of SOD and Ca were increased after OLP treatment. Furthermore, OLP could reduce the oxidative stress of the renal tissues, decrease the expression levels of pro-inflammatory factors through TLR4-mediated MAPK and NF-κB pathway, inhibit the apoptosis of renal cells by MAPK pathway, and relieve the renal fibrosis by down-regulating the expression of TGF-β1.

Conclusions: OLP is composed of three kinds of monosaccharides and →4)-Glcp-(1→ is the main glycosidic linkage in the polysaccharide. OLP could ameliorate CKD in mice by declining the oxidative stress, inflammation, apoptosis and fibrosis in the kidneys. The study provided some evidences for the potential application of OLP in alleviating CKD.
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http://dx.doi.org/10.1016/j.jep.2021.114189DOI Listing
August 2021

Pathways and mechanisms of nitrogen transformation during co-composting of pig manure and diatomite.

Bioresour Technol 2021 Jun 1;329:124914. Epub 2021 Mar 1.

College of Natural Resources and Environment, Northwest A&F University, Yangling 712100, PR China. Electronic address:

The aim of this study was to investigate the pathways and mechanisms of nitrogen transformation during the composting process, by adding diatomite (0%, 2.5%, 5%, 10%, 15% and 20%) into initial mixtures of pig manure and sawdust. The results revealed that diatomite facilitated the conversion from NH-N to amino acid nitrogen and hydrolysis undefined nitrogen, then reduced NH and NO emission by 8.63-35.29% and 14.34-73.21%, respectively. Moreover, the structure and abundance of nitrogen functional genes provided evidence for nitrogen loss. Furthermore, compared with the control (0.03), the treatment blended with 10% diatomite (T3) had the highest value in composting score (-1.27). Additionally, the ratio of carbon and nitrogen (57.30%) was vital for reducing nitrogen loss among all physio-chemical parameters in this study. In conclusion, adding diatomite was a practical way to enhance nitrogen conservation and increase quality of end products, and the optimum added dosage was at 10%.
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http://dx.doi.org/10.1016/j.biortech.2021.124914DOI Listing
June 2021

Double emulsions with ultrathin shell by microfluidic step-emulsification.

Lab Chip 2021 04;21(8):1613-1622

School of Aerospace Engineering, Beijing Institute of Technology, ZhongGuanCunNan Street #5, 100081, Beijing, China.

Double emulsions with ultrathin shells are important in some biomedical applications, such as controlled drug release. However, the existing production techniques require two or more manipulation steps, or more complicated channel geometry, to form thin-shell double emulsions. This work presents a novel microfluidic tri-phasic step-emulsification device, with an easily fabricated double-layer PDMS channel, for production of oil-in-oil-in-water and water-in-water-in-oil double emulsions in a single step. The shell thickness is controlled by the flow rates and can reach 1.4% of the μm-size droplet diameter. Four distinct emulsification regimes are observed depending on the experimental conditions. A theoretical model for the tri-phasic step-emulsification is proposed to predict the boundaries separating the four regimes of emulsification in plane of two dimensionless capillary numbers, Ca. The theory yields two coupled nonlinear differential equations that can be solved numerically to find the approximate shape of the free interfaces in the shallow (Hele-Shaw) microfluidic channel. This approximation is then used as the initial guess for the more accurate finite element method solution, showing very good agreement with the experimental findings. This study demonstrates the feasibility of co-flow step-emulsification as a promising method to production of double (and multiple) emulsions and micro-capsules with ultrathin shells of controllable thickness.
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http://dx.doi.org/10.1039/d0lc01044hDOI Listing
April 2021

Improvement of humification and mechanism of nitrogen transformation during pig manure composting with Black Tourmaline.

Bioresour Technol 2020 Jul 23;307:123236. Epub 2020 Mar 23.

College of Natural Resources and Environment, Northwest Agriculture and Forestry University, Yangling 712100, PR China. Electronic address:

This study aimed to reveal the role of Black Tourmaline (TM) in the humification enhancement and the mechanism of nitrogen transformation during pig manure composting. Results showed that adding TM promoted the maturity and improved the humification degree by 20.13-33.77%. And TM was beneficial for the transformation from NH-N to amino organic nitrogen to fix more nitrogen in compost mass. Then NH and NO volatilization were decreased by 22.88-34.76% and 69.79-87.47% by comparison with the control, and the minimum value in nitrogen loss (26.78%) was observed in the 10% TM blended treatment. Furthermore, through RDA analysis, the protease an upmost contributor to nitrogen transformation. Meanwhile, total organic carbon was dominant factor affected enzymatic activities. Therefore, 10% TM was suggested to reduce nitrogen loss and increase humification in this research. Deeply related research in gene and specific addition amounts of TM will be investigated later.
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http://dx.doi.org/10.1016/j.biortech.2020.123236DOI Listing
July 2020

Convolutional neural network to predict the local recurrence of giant cell tumor of bone after curettage based on pre-surgery magnetic resonance images.

Eur Radiol 2019 Oct 11;29(10):5441-5451. Epub 2019 Mar 11.

Radiology Department, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, HaiNing Rd.100, Shanghai, 200080, China.

Objective: To predict the local recurrence of giant cell bone tumors (GCTB) on MR features and the clinical characteristics after curettage using a deep convolutional neural network (CNN).

Methods: MR images were collected from 56 patients with histopathologically confirmed GCTB after curettage who were followed up for 5.8 years (range, 2.0 to 9.5 years). The inception v3 CNN architecture was fine-tuned by two categories of the MR datasets (recurrent and non-recurrent GCTB) obtained through data augmentation and was validated using fourfold cross-validation to evaluate its generalization ability. Twenty-eight cases (50%) were chosen as the training dataset for the CNN and four radiologists, while the remaining 28 cases (50%) were used as the test dataset. A binary logistic regression model was established to predict recurrent GCTB by combining the CNN prediction and patient features (age and tumor location). Accuracy and sensitivity were used to evaluate the prediction performance.

Results: When comparing the CNN, CNN regression, and radiologists, the accuracies of the CNN and CNN regression models were 75.5% (95% CI 55.1 to 89.3%) and 78.6% (59.0 to 91.7%), respectively, which were higher than the 64.3% (44.1 to 81.4%) accuracy of the radiologists. The sensitivities were 85.7% (42.1 to 99.6%) and 87.5% (47.3 to 99.7%), respectively, which were higher than the 58.3% (27.7 to 84.8%) sensitivity of the radiologists (p < 0.05).

Conclusion: The CNN has the potential to predict recurrent GCTB after curettage. A binary regression model combined with patient characteristics improves its prediction accuracy.

Key Points: • Convolutional neural network (CNN) can be trained successfully on a limited number of pre-surgery MR images, by fine-tuning a pre-trained CNN architecture. • CNN has an accuracy of 75.5% to predict post-surgery recurrence of giant cell tumors of bone, which surpasses the 64.3% accuracy of human observation. • A binary logistic regression model combining CNN prediction rate, patient age, and tumor location improves the accuracy to predict post-surgery recurrence of giant cell bone tumors to 78.6%.
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http://dx.doi.org/10.1007/s00330-019-06082-2DOI Listing
October 2019

Magnetic solid-phase extraction using sulfur-containing functional magnetic polymer for high-performance liquid chromatography-inductively coupled plasma-mass spectrometric speciation of mercury in environmental samples.

J Chromatogr A 2019 Jun 23;1595:19-27. Epub 2019 Feb 23.

Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, PR China.

1,2-Ethanedithiol was firstly involved in preparing sulfur-containing functional magnetic polymer material of [email protected]@ glycidyl methacrylate (GMA)-S-SH. The prepared [email protected]@GMA-S-SH magnetic nanoparticles (MNPs) show better adsorption capacity and adsorption/desorption dynamics for mercury species than other -SH functional materials due to the high affinity of both thioether and thiol group towards mercury. A new method of magnetic solid phase extraction (MSPE) using [email protected]@GMA-S-SH as sorbents combined with high performance liquid chromatography (HPLC)-inductively coupled plasma mass spectrometry (ICP-MS) was developed for the analysis of trace Hg, methylmercury (MeHg) and phenylmercury (PhHg). Variables affecting the simultaneous extraction of three target mercury species were investigated. It was found that Hg, MeHg and PhHg in 200 mL of water sample could be quantitatively adsorbed within 5 min and eluted by 0.5 mL 0.1 mol L nitric acid and 4% thiocarbamide in 2 min. The developed method has high actual enrichment factors of 367, 380, 329-fold for Hg, MeHg and PhHg, respectively. The detection limits were 0.40, 0.49 and 1.4 ng L with the relative standard deviations (n = 5, inter-day) of 7.4, 8.1 and 8.3% for Hg, MeHg and PhHg, respectively. The accuracy of the method was validated by the analysis of the Certified Reference Materials of GSS-11 soil, GSS-13 soil, GSB21 rice and DORM-2 fish. The established method was successfully used for the mercury speciation in farmland water, soil and rice samples collected from Wanshan, China, and satisfactory recoveries (84.3-116%) were obtained for the spiked samples.
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http://dx.doi.org/10.1016/j.chroma.2019.02.050DOI Listing
June 2019

CA125 over-release behavior following a 75-g oral glucose test as a predictive biomarker of multidrug resistance in patients with ovarian cancer.

Int J Cancer 2019 09 23;145(6):1690-1700. Epub 2019 Mar 23.

Department of Obstetrics and Gynecology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Multidrug resistance is a major cause of death in patients with ovarian cancer. To improve patient survival, we developed a novel, noninvasive and convenient tool, the 75-gram oral glucose (75gOG)-stimulated CA125 test, to monitor cancer chemoresistance in real time. Our in vitro proof-of-principal experiments revealed that post-75gOG glucose and insulin peaks can synergistically increase cancer-derived CA125 levels, and the increase in CA125 secretion (ΔCA125) is correlated with the overactivation of the insulin receptor (IR)-PI3K-Akt axis and increases (ΔIC s) in cisplatin/taxol IC s. Correspondingly, among the 93 patients enrolled, post-75gOG CA125 over-release (i.e., enhanced ΔCA125) behavior was associated with overexpression of the IR-PI3K-Akt pathway and its downstream components, namely, IR, pAkt, pS6 and GLUT4, in cancer specimens. Furthermore, both pre- and postsurgical 75gOG CA125 tests demonstrated that CA125 over-release showed excellent prediction efficacy on the chemoresistance potential of ovarian cancer; notably, the former indicated the need for an optimal debulking surgery, and the latter suggested the use of IR-PI3K-Akt inhibitors. Both test results possess independent prognostic significance for the 2-year progression-free survival (PFS) and overall survival (OS) of patients. The odds ratios and corresponding 95% confidence intervals (95% CIs) were 2.680 (95% CI: 1.393-5.156) for patients with CA125 over-release behavior evidenced by a presurgical 75gOG CA125 test or 3.822 (95% CI: 1.942-7.522) for that evidenced by a postsurgical test in PFS; and 3.320 (95% CI: 1.508-7.309) for patients with CA125 over-release behavior evidenced by a presurgical test or 5.212 (95% CI: 2.241-12.121) for that evidenced by a postsurgical test in OS.
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http://dx.doi.org/10.1002/ijc.32237DOI Listing
September 2019

Tumor immunohistochemistry and preoperative magnetic resonance imaging features predict local recurrence of giant cell tumor of bone following intralesional curettage.

Oncol Lett 2019 Feb 30;17(2):1425-1434. Epub 2018 Nov 30.

Department of Radiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P.R. China.

Giant cell tumor of bone (GCTB) is among the most prevalent types of tumor of the bone in East and Southeast Asia. The high rate of local recurrence following intralesional curettage poses a challenge for the clinical treatment of GCTB. In the present study, the expression of matrix metalloproteinase-9 (MMP-9), vascular endothelial growth factor (VEGF), receptor activator of nuclear factor-κB (RANK) and RANK ligand (RANKL) in GCTB was investigated by immunohistochemical staining. A correlation between expression and preoperative magnetic resonance imaging (MRI) features was identified. The patients were followed up for ≥2 years after intralesional curettage. The rates of local recurrence were compared among different groups. A total of 74 cases of GCTB in the proximal tibia or distal femur were investigated. MRI features were retrospectively examined by correlation analysis. The expression of MMP-9 was demonstrated to be associated with cystic changes and the 'paint brush borders' sign (P<0.05), and positively associated with the expression of RANKL (P<0.05) and VEGF (P<0.05). Cystic changes, the 'paint brush borders' sign and adjacent soft tissue invasion were associated with high rates of local recurrence (P<0.05) and were therefore identified as significant risk factors. Pathologically, the 'paint brush borders' sign was demonstrated to be indicative of local invasion of the bone. These findings may be useful for predicting local recurrence of GCTB, and may provide important insight into the preoperative MRI features and molecular expression patterns of GCTB tumors.
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http://dx.doi.org/10.3892/ol.2018.9778DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6341842PMC
February 2019

Information Fusion for Human Action Recognition via Biset/Multiset Globality Locality Preserving Canonical Correlation Analysis.

IEEE Trans Image Process 2018 Nov 12;27(11):5275-5287. Epub 2018 Jul 12.

In this paper, we study the problem of human action recognition, in which each action is captured by multiple sensors and represented by multisets. We propose two novel information fusion techniques for fusing the information from multisets. The first technique is biset globality locality preserving canonical correlation analysis (BGLPCCA), which aims to learn the common feature subspace between two sets. The second technique is multiset globality locality preserving canonical correlation analysis (MGLPCCA), which aims to deal with three or more sets. The proposed BGLPCCA and MGLPCCA are able to learn a low-dimensional common subspace that preserves the local and global structures of data samples. Moreover, two novel descriptors are presented for both depth and skeleton. We then propose a new human action recognition framework employing the proposed BGLPCCA or MGLPCCA to learn the shared subspace from multiple sets of features including skeleton, depth, and optical flow. Extensive experiments on five publicly available datasets (MSR Action3D, UTD multimodal human action dataset, multimodal action database, Kinect activity recognition dataset, and SBU Kinect interaction dataset) demonstrate the effectiveness of the proposed framework.
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http://dx.doi.org/10.1109/TIP.2018.2855438DOI Listing
November 2018

Postprandial increase in serum CA125 as a surrogate biomarker for early diagnosis of ovarian cancer.

J Transl Med 2018 05 1;16(1):114. Epub 2018 May 1.

Department of Obstetrics and Gynecology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 160 Pujian Road, Shanghai, 200127, China.

Background: CA125 is a prevalently used serum biomarker for detecting ovarian cancer over the last three decades. However, it has a significant deficiency in screening for early-stage cancer. With the purpose of exploring an effective approach to improve its performance in early diagnosis, we investigated the postprandial fluctuation pattern of cancer-derived CA125 and the underlying mechanism.

Methods: In two medical centers, 551 patients sonographically diagnosed with ovarian (adnexal) cysts (< 5 cm in diameter) were enrolled and divided into five disease groups (pelvic inflammatory cysts, retention cysts, endometrioma, benign/borderline cystadenoma and malignant cysts). The subtle differences in 1-h postprandial serum CA125 increases were compared between disease groups. A support vector machine (SVM)-based algorithm was used for refining the performance of CA125 postprandial increment. Ovarian cancer xenograft animal and cancer cell models were used to recapitulate the clinical findings and reveal the molecular basis of postprandial blood glucose and insulin in invoking the synthesis/secretion/re-absorption of CA125.

Results: Patients with ovarian cancer presented the highest postprandial increment 13.3 ± 0.7% (mean ± standard deviation) among the five disease groups. Using a CA125 increment ≥ 10% criterion, the sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) reached 83.3, 96.3, 61.1 and 98.8%, respectively, for early-stage ovarian cancer. This performance was further improved by the SVM-based CA125-increment algorithm, which exhibited 91.7% sensitivity, 99.2% specificity, 89.2% PPV and 99.4% NPV. Both modalities manifested diagnostic advantages over the traditional CA125 test (75.0% sensitivity, 25.4% specificity, 6.6% PPV and 93.6% NPV at the cut-off of 35 U/mL). Regarding the molecular basis, the postprandial blood glucose and insulin-invoked overexpression of Mucin 16 (encoding CA125) were demonstrated in animal and cancer cell models, which were mediated by the PI3K-Akt pathway. Nevertheless, a Mesothelin-based CA125 re-absorption behavior was noted in the treated cancer cells, which contributed to the over-drop following the postprandial peak of serum CA125.

Conclusions: Cancer-derived serum CA125 possesses a unique and distinctive postprandial pattern, that distinguishes it from the common CA125 elevation in a benign disease condition. The dynamic measurement/assessment strategy can achieve a discriminatory power superior to that of a static test.
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http://dx.doi.org/10.1186/s12967-018-1489-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5930842PMC
May 2018

Matrix metalloproteinase-9 expression of GCTSC in peripheral tissue and central tissue of GCTB.

J Cell Biochem 2018 07 30;119(7):5805-5812. Epub 2018 Mar 30.

Department of Radiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Giant cell tumor stromal cell (GCTSC) is the tumor cell of giant cell tumor of bone (GCTB). The biomarkers characterization of GCTSC is critical for the selection of GCTB targeting drugs. We believe the main functions of GCTSC in different part of tumor should be different for different environment. Then the biological behavior and molecular biomarkers of GCTSC should be different as well. Based on this idea, we focused on GCTSC which located in central tissue, peripheral tissue and took MMP-9 as the breakthrough point to carry out research. The results showed MMP-9 staining grade of GCTSC which located in central tissue was slight, whereas multinucleated giant cell staining grade was high. The peripheral tissue was consisted by almost GCTSC with high MMP-9 staining degree and mRNA expression. This study also provided clues and inspiration for reducing GCTB recurrence rate after intralesional curettage with MMP-9 targeted therapy which were aimed at the residual peripheral tissue.
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http://dx.doi.org/10.1002/jcb.26766DOI Listing
July 2018

Retrospective investigation of "paint brush borders" sign in association with local recurrence of giant cell tumor of bone after intralesional curettage.

J Bone Oncol 2018 Mar 27;10:41-48. Epub 2017 Nov 27.

Professor of Department of Radiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

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http://dx.doi.org/10.1016/j.jbo.2017.11.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5725224PMC
March 2018

Magnetic resonance feature of "paintbrush borders" sign as a novel way to predict recurrence of giant cell tumor of bone after curettage: a pilot study.

J Int Med Res 2018 Feb 12;46(2):710-722. Epub 2017 Sep 12.

1 Department of Radiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Objective To identify the prognostic factors for local recurrence of giant cell tumor of bone (GCTB) through assessment of the preoperative imaging features of the tumor border. Methods Patients with GCTBs treated with intralesional procedures in the proximal tibia and distal femur were prospectively enrolled and then followed up for at least 2 years. The GCTBs were grouped according to their preoperative imaging features. GCTBs treated with en bloc resection were enrolled for investigation of the pathologic basis of specific imaging features. Differences between rates were evaluated by the chi-square test or Fisher's exact test; independent factors were identified by multivariate logistic regression analysis. Results Fifty-three patients were enrolled and successfully followed up. Relapse occurred in 22 patients. Patients with a "paintbrush borders" sign (n = 21) had a significantly higher rate of local recurrence (71.43%) than patients without this sign (21.88%). The "paintbrush borders" sign was identified as an independent prognostic factor for local recurrence. Other imaging features were not significantly associated with recurrence. The "paintbrush borders" sign showed a correlation with local invasion of bone. Conclusion The "paintbrush borders" sign on preoperative magnetic resonance imaging is an independent prognostic factor for local recurrence of GCTB.
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http://dx.doi.org/10.1177/0300060517720345DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5971501PMC
February 2018

Expression of Wnt3a in hepatocellular carcinoma and its effects on cell cycle and metastasis.

Int J Oncol 2017 Oct 1;51(4):1135-1145. Epub 2017 Sep 1.

Laboratory of Hepatobiliary Surgery, Affiliated Hospital of Guangdong Medical University, Zhanjiang Key Laboratory of Hepatobiliary Diseases, Zhanjiang, Guangdong 524001, P.R. China.

Invasion and metastasis are the primary causes of mortality from hepatocellular carcinoma (HCC). Effective inhibition against participants in the tumourigenesis and metastasis process is critical for treatment of HCC. Wnt3a is involved in the development and metastasis of many malignant tumours. However, the specific mechanisms of Wnt3a-mediated cell proliferation, invasion and metastasis in HCC remain unclear. In this study, we found that Wnt3a and its target gene c‑Myc showed higher expression in tumour tissues than normal liver tissues in HCC patients; 71.8% of the cases studied had high Wnt3a and c‑Myc expression levels (n=32); Wnt3a expression positively correlated with its target genes MMP‑7 and c‑Myc. Intriguingly, the expression of Wnt3a, MMP‑7 and c‑Myc is significantly correlated with Notch3 and Hes1 expression. In vitro experiments showed that Wnt3a was highly expressed in MHcc97H and SK‑Hep‑1 cells. Therefore, Wnt3a expression was silenced with siRNA, and then, MTT, flow cytometry, wound healing and Transwell assays were performed to analyse cell proliferation, cycle, migration and invasion. The results demonstrated that downregulation of Wnt3a expression inhibited cell viability and induced G0/G1 cell cycle arrest via decreased expression of cyclin D1 and c‑Myc and increased expression of p21 and p27. In addition, deletion of Wnt3a significantly inhibited migration and invasion by downregulating MMP‑2/-7/-9 expression via the MAPK (p38, ERK1/2 and JNK) pathway. In conclusion, our data show that Wnt3a is involved in HCC development. Wnt3a may be an effective target for treatment of HCC.
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http://dx.doi.org/10.3892/ijo.2017.4112DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5592886PMC
October 2017

CD47 promotes ovarian cancer progression by inhibiting macrophage phagocytosis.

Oncotarget 2017 Jun;8(24):39021-39032

Department of Immunology, Tianjin Key Laboratory of Cellular and Molecular Immunology, Key Laboratory of Educational Ministry of China, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, 300071, China.

Targeting CD47 efficiently enhances macrophage phagocytosis in both physiological and pathological conditions. Anti-CD47 antibodies have been shown to inhibit the progression of several types of cancer. However, the mechanism of anti-CD47 monoclonal antibody (mAb) treatment remains controversial. In this study, we confirmed that CD47 protein is highly expressed in ovarian cancer, and is correlated with poor clinical characteristics and prognosis. CD47 knockdown in the ovarian cancer cell line, SK-OV-3, promoted phagocytosis by macrophages in vitro and inhibited tumor growth in vivo. These data combined suggest that CD47 inhibition is a potential strategy for cancer treatment. Using an anti-CD47 mAb, we found that CD47 inhibition in both SK-OV-3 cells and primary cancer cells was able to recapitulate our knockdown results and led to an increase in the number of infiltrating macrophages. In addition, the CD133+ tumor initiating cells expressed a high level of CD47, and anti-CD47 mAb treatment was able to trigger the phagocytosis of this cell population. In conclusion, our results indicate that CD47 inhibits macrophage phagocytosis of ovarian cancer cells, and down-regulation of CD47 or inhibiting CD47 by mAb was able to reverse the negative effect. Thus, CD47 antibody therapy may be a promising strategy to treat ovarian cancer.
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http://dx.doi.org/10.18632/oncotarget.16547DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5503592PMC
June 2017

Erratum to: A prospective study on predicting local recurrence of giant cell tumour of bone by evaluating preoperative imaging features of the tumour around the knee joint.

Radiol Med 2017 07;122(7):556

Department of Radiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197, Rui Jin Er Road, Shanghai, 200025, China.

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http://dx.doi.org/10.1007/s11547-017-0760-8DOI Listing
July 2017

Prognosis of local recurrence in giant cell tumour of bone: what can we do?

Radiol Med 2017 Jul 7;122(7):505-519. Epub 2017 Mar 7.

Department of Radiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Giant cell tumour of bone (GCTB) is classified as an intermediate tumour with rare metastasis, but is challenged by local recurrence. This review focuses on the role of radiological evaluation in terms of prognosis of local recurrence in GCTB. We hope to highlight the value of radiological evaluation by integrating studies on the impact of surgical treatments and non-surgical factors on local recurrence of GCTB and the current statuses of genetic and molecular prognostic factors of GCTB. Radiological evaluation can provide diverse information on tumours. As a non-invasive method, magnetic resonance imaging (MRI) is especially valuable for the diagnosis and evaluation of bone tumours due to its heightened sensitivity to soft tissue disease and multiplanar image acquisition. Imaging findings should be integrated with clinical characteristics, pathology and genetic and molecular prognostic factors to direct clinical approach and reduce the local recurrence of GCTB. Therefore, it is necessary to establish a multi-perspective evaluation system by which prognostic factors can be reliably determined. We further advocate more large-scale prospective studies. With the help of radiological evaluation, the clinic treatment of GCTB can be guided and local recurrence might be reduced; additionally, MR imaging can identify local recurrence of GCTB after surgical treatment in the early stage.
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http://dx.doi.org/10.1007/s11547-017-0746-6DOI Listing
July 2017

A prospective study on predicting local recurrence of giant cell tumour of bone by evaluating preoperative imaging features of the tumour around the knee joint.

Radiol Med 2017 Jul 7;122(7):546-555. Epub 2017 Mar 7.

Department of Radiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197, Rui Jin Er Road, Shanghai, 200025, China.

Purpose: To evaluate the role of medical imaging in predicting local recurrence of giant cell tumour of bone (GCTB) by assessing the preoperative imaging features of GCTB around the knee.

Methods: Forty-eight consecutive GCTBs in the proximal tibia and distal femur treated with curettage were prospectively enrolled. Patients were grouped in terms of their imaging features on radiography, computed tomography (CT) and magnetic resonance imaging (MRI). All patients were followed up for at least two years after surgery. The association between preoperative imaging features and local recurrence was investigated. Imaging features were retrospectively studied by correlation analysis. The differences between rates were tested by the Chi square and Fisher exact tests; independent factors were determined by multivariate logistic regression analysis.

Results: Cystic change and adjacent soft tissue invasion were associated with a higher rate of local recurrence compared to the negative groups (P < 0.05). Cystic change was identified as an independent risk factor for local recurrence of GCTB (P < 0.05). Expansibility was correlated with the "soap bubble" sign and the fluid-fluid level (P < 0.05); the "soap bubble" sign was correlated with osteosclerosis and the fluid-fluid level (P < 0.05); cortical bone involvement was correlated with adjacent soft tissue invasion (P < 0.05); and cystic change was correlated with the fluid-fluid level (P < 0.05).

Conclusion: Cystic change was an independent risk factor for local recurrence of GCTB. Adjacent soft tissue invasion might indirectly relate to local relapse. A cluster of association relationships between imaging features was revealed.
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http://dx.doi.org/10.1007/s11547-017-0745-7DOI Listing
July 2017

Tunable Yb:CaF2-SrF2 laser and femtosecond mode-locked performance based on semiconductor saturable absorber mirrors.

Appl Opt 2016 Oct;55(29):8359-8362

We experimentally demonstrate an effective continuous-wave tunable operation and femtosecond passively mode-locked pulse from a Yb:CaF2-SrF2 mixed crystal laser for the first time. Pumped by a 977 nm fiber-coupled laser diode, a continuous-wave mode-locked pulse as short as 634 fs was generated. The shortest pulse operated under a repetition rate of 87 MHz at the central wavelength of 1047 nm. The calculated time-bandwidth product was 0.503, which was 1.6 times the Fourier transform-limited sech2-shaped pulses.
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http://dx.doi.org/10.1364/AO.55.008359DOI Listing
October 2016

The changing 50% inhibitory concentration (IC50) of cisplatin: a pilot study on the artifacts of the MTT assay and the precise measurement of density-dependent chemoresistance in ovarian cancer.

Oncotarget 2016 Oct;7(43):70803-70821

Department of Obstetrics and Gynecology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.

Inconsistencies in the half-maximal (50%) inhibitory concentration (IC50) data for anticancer chemotherapeutic agents have yielded irreproducible experimental results and thus reciprocally contradictory theories in modern cancer research. The MTT assay is currently the most extensively used method for IC50 measurements. Here, we dissected the critical reasons behind MTT-dependent IC50 inconsistencies. We showed that IC50 errors caused by the technical deficiencies of the MTT assay are large and not adjustable (range: 300-11,000%). To overcome severe MTT artifacts, we developed an unbiased direct IC50 measurement method, the limiting dilution assay. This detection technique led us to the discovery of the inherent density-dependent chemoresistance variation of cancer cells, which is manifold and unpredictable in its forms. The subsequent intracellular signaling pathway analysis indicated that pAkt and p62 expression levels correlated with alterations in the IC50 values for cisplatin in ovarian cancer, providing an explainable mechanism for this property. An in situ pAkt-and-p62-based immunohistochemical (IHCpAkt+p62) scoring system was thereby established. Both the limiting dilution assay and the IHCpAkt+p62 scoring system accurately predicted the primary chemoresistance against cisplatin in ovarian cancer patients. Furthermore, two distinct chemoresistant recurrence patterns were uncovered using these novel detection tools, which were linked to two different forms of density-chemoresistance relationships (positively vs. negatively correlated), respectively. An interpretation was given based on the cancer evolution theory. We concluded that the density-related IC50 uncertainty is a natural property of the cancer cells and that the precise measurement of the density-dependent IC50 spectrum can benefit both basic and clinical cancer research fields.
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http://dx.doi.org/10.18632/oncotarget.12223DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5342590PMC
October 2016

Colloid-associated plutonium aged at room temperature: evaluating its transport velocity in saturated coarse-grained granites.

J Contam Hydrol 2015 Jan 4;172:24-32. Epub 2014 Nov 4.

Northwest Institute of Nuclear Technology, P.O. Box 69-14, Xi'an City, Shanxi Province 710024, PR China.

The fate and transport of colloidal contaminants in natural media are complicated by physicochemical properties of the contaminants and heterogeneous characteristics of the media. Size and charge exclusion are two key microscopic mechanisms dominating macroscopic transport velocities. Faster velocities of colloid-associated actinides than that of (3)H2O were consistently indicated in many studies. However, dissociation/dissolution of these sorbed actinides (e.g., Pu and Np), caused by their redox reactions on mineral surfaces, possibly occurred under certain chemical conditions. How this dissolution is related to transport velocities remains unanswered. In this study, aging of the colloid-associated Pu (pseudo-colloid) at room temperature and transport through the saturated coarse-grained granites were performed to study whether Pu could exhibit slower velocity than that of (3)H2O (UPu/UT <1). The results show that oxidative dissolution of Pu(IV) associated with the surfaces of colloidal granite particles took place during the aging period. The relative velocity of UPu/UT declined from 1.06 (unaged) to 0.745 (135 d) over time. Size exclusion limited to the uncharged nano-sized particles could not explain such observed UPu/UT <1. Therefore, the decline in UPu/UT was ascribed to the presence of electrostatic attraction between the negatively charged wall of granite pore channels and the Pu(V)O2(+), as evidenced by increasing Pu(V)O2(+) concentrations in the suspensions aged in sealed vessels. As a result of this attraction, Pu(V)O2(+) was excluded from the domain closer to the centerline of pore channels. This reveals that charge exclusion played a more important role in dominating UPu than the size exclusion under the specific conditions, where oxidative dissolution of colloid-associated Pu(IV) was observed in the aged suspensions.
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http://dx.doi.org/10.1016/j.jconhyd.2014.10.011DOI Listing
January 2015

Combined laparoscopy and hysteroscopy vs. uterine curettage in the uterine artery embolization-based management of cesarean scar pregnancy: a cohort study.

Int J Clin Exp Med 2014 15;7(9):2793-803. Epub 2014 Sep 15.

Key Laboratory of Female Reproductive Endocrine Related Diseases, Obstetrics and Gynecology Hospital, Fudan University Shanghai, China.

Aim: To evaluate the efficacy of combined laparoscopy and hysteroscopy compared with traditional uterine curettage in removing the ectopic conceptus and repairing the tissue defect following uterine artery embolization (UAE) management of cesarean scar pregnancy (CSP).

Design: A prospective cohort study.

Setting: Three large obstetrics and gynecology centers in Shanghai, China.

Sample: CSP patients diagnosed between March 2009 and August 2010 who had received no prior treatments, were hemodynamically stable, and had no contraindications for UAE were enrolled.

Methods: Patients were divided into two cohorts to undergo the intra-arterial methotrexate (MTX), UAE, and one of the following treatments: combined laparoscopy and hysteroscopy (research group, 25 cases) and uterine curettage (control group, 33 cases).

Main Outcome Measures: The conceptus removal rate, the severity of intra- and postoperative complications, surgical time, and duration of hospital stay.

Results: The single-surgery conceptus removal rate reached 100% in the research group, which was significantly higher than the 82% (P=0.024) observed in the control group (with one hysterectomy). The average volume of intraoperative blood loss was 78.0 mL in the research group, which was much less than the 258.5 mL (P=0.004) in the control group. Moreover, the research group had significantly shorter hospital stays and β-hCG regression times, as well as lower rates of postoperative abdominal pain, uterine bleeding and menstruation abnormalities.

Conclusions: Combined laparoscopy and hysteroscopy is much safer and more effective than uterine curettage as a supplementary measure following UAE.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4211791PMC
October 2014

Combined laparoscopy and hysteroscopy vs. uterine curettage in the uterine artery embolization-based management of cesarean scar pregnancy: a retrospective cohort study.

BMC Womens Health 2014 Sep 24;14:116. Epub 2014 Sep 24.

Department of Obstetrics and Gynecology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 160 Pujian Road, Shanghai 200127, China.

Background: The number of cesarean scar pregnancy (CSP) has significantly increased in the recent decade. Although uterine artery embolization (UAE) has been adopted to minimize the blood loss during uterine curettage removing of CSP, massive bleeding and uterine rupture can still be frequently encountered. The aim of this study was to compare the efficacy and safety of a novel combined laparoscopy and hysteroscopy technique with the traditional curettage in removing the conceptus and repairing the incision defect following the UAE management of CSP.

Methods: The CSP patients (n = 58) diagnosed between March 1, 2005 and March 1, 2010 were enrolled in three medical centers in Shanghai, China. All of these patients have undergone intra-arterial methotrexate, UAE and one of the following treatments: combined laparoscopy and hysteroscopy (study group, n = 25) and uterine curettage (control group, n = 33). Their medical records and 2-year outcomes were reviewed. The CSP removal rate, amount of blood loss during the treatment, incision repair rate (note: the post-curettage healing process of the incision defect was seen as a form of natural incision repairing, i.e., the self-repair mode), hospital stay, β-hCG regression time and postoperative sequelae were compared between two groups.

Results: The CSP removal rate in the study group (100%) was significantly higher than that (79%) in the control group (p = 0.024). The average blood loss was 78.0 mL in the study group, which was much less than the 258.5 mL (p = 0.004) in the control group. A satisfactory incision repair rate (96%) was achieved in the study group, while it was 25% (p < 0.001) in the control group. Moreover, the study group had significantly shorter hospital stays (p = 0.043) and β-hCG regression times (p = 0.033), lower rates of postoperative abdominal pain (p = 0.035) and menstruation abnormalities (p = 0.043).

Conclusions: Combined laparoscopy and hysteroscopy is much safer and more effective than uterine curettage as a supplementary measure to remove the conceptus and repair the cesarean incision following the UAE management of CSP.
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http://dx.doi.org/10.1186/1472-6874-14-116DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4179841PMC
September 2014

FOXM1 modulates cisplatin sensitivity by regulating EXO1 in ovarian cancer.

PLoS One 2014 13;9(5):e96989. Epub 2014 May 13.

Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China; Shanghai Key Laboratory of Gynecologic Oncology, Shanghai, China; Focus Construction Subject of Shanghai Education Department, Shanghai, China; Shanghai Health Bureau Key Disciplines and Specialties Foundation, Shanghai, China.

Cisplatin is commonly used in ovarian cancer chemotherapy, however, chemoresistance to cisplatin remains a great clinical challenge. Oncogenic transcriptional factor FOXM1 has been reported to be overexpressed in ovarian cancer. In this study, we aimed to investigate the potential role of FOXM1 in ovarian cancers with chemoresistance to cisplatin. Our results indicate that FOXM1 is upregulated in chemoresistant ovarian cancer samples, and defends ovarian cancer cells against cytotoxicity of cisplatin. FOXM1 facilitates DNA repair through regulating direct transcriptional target EXO1 to protect ovarian cancer cells from cisplatin-mediated apoptosis. Attenuating FOXM1 and EXO1 expression by small interfering RNA, augments the chemotherapy efficacy against ovarian cancer. Our findings indicate that targeting FOXM1 and its target gene EXO1 could improve cisplatin effect in ovarian cancer, confirming their role in modulating cisplatin sensitivity.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0096989PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4019642PMC
January 2015

A high M1/M2 ratio of tumor-associated macrophages is associated with extended survival in ovarian cancer patients.

J Ovarian Res 2014 Feb 8;7:19. Epub 2014 Feb 8.

Department of Obstetrics and Gynecology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 160 Pujian Road, Shanghai 200127, China.

Background: Tumor-associated macrophages (TAMs) are classified into two major phenotypes, M1 and M2. M1 TAMs suppress cancer progression, while M2 TAMs promote it. However, little is known regarding the role of TAMs in the development of ovarian cancer. Here, we investigated the relationship between TAM distribution patterns (density, microlocalization, and differentiation) and ovarian cancer histotypes, and we explored whether altered TAM distribution patterns influence long-term outcomes in ovarian cancer patients.

Methods: A total of 112 ovarian cancer patients were enrolled in this study, and the subjects were divided into two groups according to their survival (< 5 years vs. ≥ 5 years). Immunohistochemistry and immunofluorescence were used to determine the density, microlocalization, and differentiation status of TAMs in ovarian cancer tissues for each histotype. Kaplan-Meier survival and multivariate Cox regression analyses were used to evaluate the prognostic significance of TAM-related parameters in ovarian cancer.

Results: TAMs most frequently infiltrated into the cancer tissue of the serous histotype, followed by mucinous, undifferentiated, endometrioid, and clear cell histotypes (p = 0.049). The islet/stroma ratio of total TAMs varied among the cancer histotypes, with mucinous and undifferentiated cancers displaying the lowest and highest ratios, respectively (p = 0.005). The intratumoral TAM density significantly increased with increasing cancer stage and grade (p = 0.023 and 0.006, respectively). However, the overall M1/M2 TAM ratio decreased as the cancer stage increased (p = 0.012). In addition, the intra-islet M1/M2 ratio inversely correlated with the residual site size (p = 0.004). Among the TAM-related parameters, only the increased overall and intra-islet M1/M2 TAM ratios displayed prognostic significance in both the Kaplan-Meier survival and multivariate Cox regression analyses; however, the values of these two parameters did not differ significantly among the cancer histotypes.

Conclusions: The patients with increased overall or intra-islet M1/M2 TAM ratios presented with an improved 5-year prognosis. Nevertheless, the TAM distribution patterns did not influence the overall outcomes of different ovarian cancer histotypes.
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http://dx.doi.org/10.1186/1757-2215-7-19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3939626PMC
February 2014

Association between cytochrome P450 2C9 gene polymorphisms and colorectal cancer susceptibility: evidence from 16 case-control studies.

Tumour Biol 2014 May 11;35(5):4317-22. Epub 2014 Jan 11.

Department of General Surgery, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai, 200032, People's Republic of China.

Previous epidemiological studies have evaluated the association between common variations of cytochrome P450 (CYP)2C9 (430C>T and 1075A>C) and the risk of colorectal cancer (CRC) with conflicting results. To derive a more precise estimation of the relationship between these CYP2C9 polymorphisms and CRC, a meta-analysis was performed. PubMed, Embase, CNKI, and Web of Science databases were searched. A total of 16 studies including 9,463 cases and 11,416 controls were identified. Potential sources of heterogeneity including ethnicity, sample size of study, genotyping method, diagnostic criteria, and outcome were systematically assessed. Overall, the summary odds ratio of 430T variant for CRC was 0.92 (95% confidence interval (CI) 0.86-0.98; P = 0.012) and 1.39 (95% CI 1.07-1.81; P = 0.013) for colorectal adenomas (CRAs). As for CYP2C9 1075A>C polymorphism, no significant results were observed in overall and subgroup analysis. There was no evidence of publication bias. In conclusion, there is evidence to indicate a significant association between CYP2C9 430C>T polymorphism and CRC/CRA risk.
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http://dx.doi.org/10.1007/s13277-013-1566-5DOI Listing
May 2014

Decreased selenium-binding protein 1 enhances glutathione peroxidase 1 activity and downregulates HIF-1α to promote hepatocellular carcinoma invasiveness.

Clin Cancer Res 2012 Jun 18;18(11):3042-53. Epub 2012 Apr 18.

Liver Cancer Institute, Fudan University, Shanghai, China.

Purpose: We aimed to characterize the role of selenium-binding protein 1 (SBP1) in hepatocellular carcinoma (HCC) invasiveness and underlying clinical significance.

Experimental Design: SBP1 expression was measured in stepwise metastatic HCC cell lines by Western blotting. The role of SBP1 in HCC was investigated using siRNA. Immunofluorescence analyses were used to detect the interaction between SBP1 and glutathione peroxidase 1 (GPX1). Nineteen fresh tumor tissues and 323 paraffin-embedded samples were used to validate in vitro findings and to detect the prognostic significance of SBP1, respectively.

Results: Inhibition of SBP1 effectively increased cell motility, promoted cell proliferation, and inhibited apoptosis only under oxidative stress; it also greatly enhanced GPX1 activity without altering GPX1 expression and downregulated hypoxia-inducible factor-1α (HIF-1α) expression. SBP1 and GPX1 formed nuclear bodies and colocalized under oxidative stress. In freshly isolated clinical HCC tissues, decreased SBP1 was linked with increased GPX1 activity and correlated with vascular invasion. Tumor tissue microarrays indicated that SBP1 was an independent risk factor for overall survival and disease recurrence; patients with lower SBP1 expression experienced shorter overall survival periods and higher rates of disease recurrence (P < 0.001). Further analyses indicated that the predictive power of SBP1 was more significant for patients beyond the Milan criteria than patients within the Milan criteria.

Conclusions: Decreased expression of SBP1 could promote tumor invasiveness by increasing GPX1 activity and diminishing HIF-1α expression in HCC; SBP1 could be a novel biomarker for predicting prognosis and guiding personalized therapeutic strategies, especially in patients with advanced HCC.
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http://dx.doi.org/10.1158/1078-0432.CCR-12-0183DOI Listing
June 2012

Estrogen receptor-regulated microRNAs contribute to the BCL2/BAX imbalance in endometrial adenocarcinoma and precancerous lesions.

Cancer Lett 2012 Jan 29;314(2):155-65. Epub 2011 Sep 29.

Department of Obstetrics and Gynecology, Fengxian Central Hospital, Shanghai 201400, PR China.

Uncontrolled estrogen exposure can induce an imbalance in BCL2/BAX expression in endometrial cells, leading to precancerous lesions and type I endometrial adenocarcinoma. This study aimed to explore the mechanism underlying this phenomenon. We show that the activated estrogen receptor can suppress the expression of BAX by upregulating a group of microRNAs including hsa-let-7 family members and hsa-miR-27a, thereby promoting an increased BCL2/BAX ratio as well as enhanced survival and proliferation in the affected cells. These ER-regulated hsa-let-7 microRNAs can be detected in most hyperplastic endometria, suggesting their potential utility as indicators of estrogen over-exposure.
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http://dx.doi.org/10.1016/j.canlet.2011.09.027DOI Listing
January 2012

Hydrazide-functionalized magnetic microspheres for the selective enrichment of digested tryptophan-containing peptides in serum.

Talanta 2011 Aug 12;85(2):1001-6. Epub 2011 May 12.

Department of Chemistry, Fudan University, Shanghai 200433, China.

The extreme complexity of protein samples is becoming a great challenge for proteomic analysis, especially for those having large dynamic range of protein abundance. To solve this problem, and to overcome the limitation of the current proteomic technologies, a new method using hydrazide-functionalized magnetic microspheres was established in this study. With this method, tryptophan (Trp)-containing peptides can be selectively and sensitively enriched from complex and low-volume samples. Furthermore, combined with 1D-LC-MS/MS analysis, the strategy was successfully applied to the proteomic study of mouse serum. The proportion of Trp-containing peptides was increased from 19.4% to 80.2% through enrichment, and the complexity of the sample was reduced more than two times. An additional 113 Trp-containing peptides and 48 novel proteins were detected compared to the conventional method. This enrichment method provides a means for identifying more proteins as potential biomarkers in serum and other complex samples.
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http://dx.doi.org/10.1016/j.talanta.2011.05.008DOI Listing
August 2011

Characterization and mechanism study of micrometer-sized secondary assembly of β-cyclodextrin.

Phys Chem Chem Phys 2011 Jan 29;13(2):447-52. Epub 2010 Oct 29.

Beijing National Laboratory for Molecular Sciences (BNLMS), Radiochemistry and Radiation Chemistry Key Laboratory of Fundamental Science, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.

We herein report a β-cyclodextrin-based secondary assembly (β-CD SA) obtained from an aqueous solution. It was found that the addition of a very small amount of organic molecule 2-phenyl-5-(4-diphenylyl) 1,3,4-oxadiazole (PBD) into an aqueous solution of 10 mM β-CD led to the formation of a micrometer-sized rodlike SA, which made the mixture turbid immediately. After careful characterization, the structure and the formation mechanism of the β-CD SA were suggested. PBD first induces β-CDs to form rigid nanotubes through head-to-head or tail-to-tail routes. Using the "solid" nanotubes as recrystallization centers, other β-CDs assembled to channel in the c axis direction and hexagonally aligned in the b axis direction, leading to the formation of a β-CD SA. In the β-CD SA, most of the β-CDs were not occupied by PBD. In the course of formation , intermolecular hydrogen-bonding plays a prominent role. The results reported herein would be helpful in constructing cyclodextrin-based architectures in water.
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http://dx.doi.org/10.1039/c0cp00899kDOI Listing
January 2011