Publications by authors named "Yifei Yan"

15 Publications

  • Page 1 of 1

Self-Oriented Empathy and Compassion Fatigue: The Serial Mediation of Dispositional Mindfulness and Counselor's Self-Efficacy.

Front Psychol 2020 8;11:613908. Epub 2021 Jan 8.

Key Laboratory of Adolescent Cyberpsychology and Behavior, Ministry of Education, Key Laboratory of Human Development and Mental Health of Hubei Province, School of Psychology, Central China Normal University, Wuhan, China.

This study aimed to explore the association between self-oriented empathy and compassion fatigue, and examine the potential mediating roles of dispositional mindfulness and the counselor's self-efficacy. A total of 712 hotline psychological counselors were recruited from the Mental Health Service Platform at Central China Normal University, Ministry of Education during the outbreak of Corona Virus Disease 2019, then were asked to complete the questionnaires measuring self-oriented empathy, compassion fatigue, dispositional mindfulness, and counselor's self-efficacy. Structural equation modeling was utilized to analyze the possible associations and explore potential mediations. In addition to reporting confidence intervals (CI), we employed a new method named model-based constrained optimization procedure to test hypotheses of indirect effects. Results showed that self-oriented empathy was positively associated with compassion fatigue. Dispositional mindfulness and counselor's self-efficacy independently and serially mediated the associations between self-oriented empathy and compassion fatigue. The findings of this study confirmed and complemented the etiological and the multi-factor model of compassion fatigue. Moreover, the results indicate that it is useful and necessary to add some training for increasing counselor's self-efficacy in mindfulness-based interventions in order to decrease compassion fatigue.
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http://dx.doi.org/10.3389/fpsyg.2020.613908DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7820531PMC
January 2021

[Corrigendum] Connexin 43 reduces susceptibility to sympathetic atrial fibrillation.

Int J Mol Med 2021 01 29;47(1):410. Epub 2020 Oct 29.

Department of Geriatric Cardiology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530000, P.R. China.

Following the publication of the above article, the authors have realized that the data shown in Fig. 3B were published previously in Fig 1A of following publication, on which several of were co‑authors [Shu C, Huang W, Zeng Z, He Y, Luo B, Liu H, Li J and Xu J: Connexin 43 is involved in the sympathetic atrial fibrillation in canine and canine atrial myocytes. Anatol J Cardiol 18: 3‑9, 2017]. This error arose inadvertently; the corrected version of Fig. 3, also containing the correct data for Fig. 3B, is shown opposite. The authors are grateful to the Editor of International Journal of Molecular Medicine for allowing them the opportunity to publish this Corrigendum, and stress that this error did not significantly influence either the results or the conclusions of the paper. Furthermore, the authors apologize to the readership for any inconvenience caused. [the original article was published in International Journal of Molecular Medicine 42: 1125-1133, 2018; DOI: 10.3892/ijmm.2018.3648].
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http://dx.doi.org/10.3892/ijmm.2020.4773DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7723675PMC
January 2021

The effects of acceptance and commitment therapy on the psychological and physical outcomes among cancer patients: A meta-analysis with trial sequential analysis.

J Psychosom Res 2021 01 20;140:110304. Epub 2020 Nov 20.

Key Laboratory of Adolescent Cyberpsychology and Behavior, Ministry of Education, Wuhan 430079, China; School of Psychology, Central China Normal University, Wuhan 430079, China; Key Laboratory of Human Development and Mental Health of Hubei Province, Wuhan 430079, China.

Objective: The current study used meta-analysis and trial sequential analysis to estimate the effects of Acceptance and Commitment Therapy (ACT) on the psychological and physical distress of cancer patients, and to identify potential moderators in this body of research.

Methods: A search of multiple databases in February 2020 identified 25 independent trials (17 randomized controlled trials, 8 non-randomized controlled trials; 2256 participants) on the effects of ACT among cancer patients. Trial sequential analysis (TSA) was used to determine whether the available evidence is sufficient to draw strong conclusions.

Results: ACT significantly reduced cancer patients' psychological distress (g = 0.88), and improved psychological flexibility (g = 0.58), quality of life (g = 1.19), and sense of hope (g = 2.17). TSA showed that there was sufficient evidence to obtain stable estimates of the effect of ACT on psychological distress and quality of life. Effect sizes for psychological distress were larger in studies conducted in eastern countries, in younger samples, and when therapy was of longer duration.

Conclusion: Acceptance and Commitment Therapy can effectively improve the mental health of cancer patients, and can be applied to clinical practice as an effective psychological intervention. Researchers are encouraged to take into account stage and trajectory of cancer in future studies.
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http://dx.doi.org/10.1016/j.jpsychores.2020.110304DOI Listing
January 2021

Structural Parameters Optimization of Elastic Cell in a Near-Bit Drilling Engineering Parameters Measurement Sub.

Sensors (Basel) 2019 Jul 30;19(15). Epub 2019 Jul 30.

College of Mechanical and Electronic Engineering, China University of Petroleum, Qingdao 266580, China.

The downhole engineering parameters measurement sub is a key component of the rotary steerable drilling system. To enable a measurement sub to serve reliably under downhole complex conditions, the structural parameters optimization of its key but weak elastic cell is systematically studied. First, the multiple relations among measurement sensitivities, structural stiffnesses, and strength during structural parameters design are summarized. Second, the selection of the structural parameters of the elastic cell is characterized as a multi-objective optimization model, which is solved using the non-dominated sorting genetic algorithm II (NSGA-II). Furthermore, the finite element method (FEM) is used to verify the measurement performance and static strength of the proposed structure. Finally, transient dynamics analysis is applied to investigate the dynamic strength of the designed structure. The results show that the proposed parameters optimization strategy can quickly obtain the database for the structural parameters design of an elastic cell. The static analysis results based on the FEM further verify the effectiveness of the proposed method. Transient dynamic analysis also reveals the relative rigor of the proposed methodology framework to some extent. This work has practical significance for improving the drilling efficiency and reducing drilling risks. In addition, this proposed methodology has good extensibility.
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http://dx.doi.org/10.3390/s19153343DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6695818PMC
July 2019

Identification of a Novel Missense Mutation in a Chinese Family With Symphalangism and Tarsal Coalitions.

Front Genet 2019 18;10:353. Epub 2019 Apr 18.

Clinic Center of Human Gene Research, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: Proximal symphalangism (SYM1) is a rare genetic bone disorder characterized by the fusion of proximal interphalangeal joints in the hands and feet. Genetic studies have identified two genes underlying SYM1 as the noggin (NOG) and the growth differentiation factor 5 (GDF5).

Case Report: In the present report, a 43-year-old gravida at 11 weeks of gestation was referred for evaluation of abnormal fusions of the joints. In the initial diagnosis, physical examination was undertaken. However, traditional radiological examination was not applied due to the need to protect the fetus, making diagnosis results inefficient to determine the exact disease affecting the proband. To acquire alternative clinical evidences, we conducted radiological examinations on two other affected family members. The radiological examination revealed that they carried the symphalangism accompanied with tarsal coalition, a very rare manifestation of SYM1. A combination of whole exome sequencing (WES) and Sanger sequencing revealed a novel heterozygous missense mutation (c.163G > T; p.Asp55Tyr) in the NOG gene, which could be associated with the observed pathogenic SYM1 in the studied family. The p.Asp55Tyr mutation co-segregated with SYM1 through the affected and unaffected family members. structural modeling of the p.Asp55Tyr mutation showed that it abolishes the interaction with the Arg167 residue and causes a change in the electrostatic potential profile of the type II binding site of the noggin protein.

Conclusion: Our findings indicate that the genetic test based on WES can be useful in diagnosing SYM1 patients, with particular advantages in preventing the fetus from contacting harmful X-ray through the traditional radiography. The novel pathogenic mutation identified would further expand our understanding of the mutation spectrum of in association with SYM1 disease and provide a guidance on how to determine whether the fetus is affected by SYM1 through the prenatal diagnosis.
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http://dx.doi.org/10.3389/fgene.2019.00353DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6499182PMC
April 2019

The sequence features that define efficient and specific hAGO2-dependent miRNA silencing guides.

Nucleic Acids Res 2018 09;46(16):8181-8196

Institut de recherche en immunologie et en cancérologie (IRIC), Université de Montréal, Montréal, Québec H3C 3J7, Canada.

MicroRNAs (miRNAs) are ribonucleic acids (RNAs) of ∼21 nucleotides that interfere with the translation of messenger RNAs (mRNAs) and play significant roles in development and diseases. In bilaterian animals, the specificity of miRNA targeting is determined by sequence complementarity involving the seed. However, the role of the remaining nucleotides (non-seed) is only vaguely defined, impacting negatively on our ability to efficiently use miRNAs exogenously to control gene expression. Here, using reporter assays, we deciphered the role of the base pairs formed between the non-seed region and target mRNA. We used molecular modeling to reveal that this mechanism corresponds to the formation of base pairs mediated by ordered motions of the miRNA-induced silencing complex. Subsequently, we developed an algorithm based on this distinctive recognition to predict from sequence the levels of mRNA downregulation with high accuracy (r2 > 0.5, P-value < 10-12). Overall, our discovery improves the design of miRNA-guide sequences used to simultaneously downregulate the expression of multiple predetermined target genes.
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http://dx.doi.org/10.1093/nar/gky546DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6144789PMC
September 2018

Connexin 43 reduces susceptibility to sympathetic atrial fibrillation.

Int J Mol Med 2018 Aug 30;42(2):1125-1133. Epub 2018 Apr 30.

Department of Geriatric Cardiology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530000, P.R. China.

Atrial fibrillation (AF) is the most common arrhythmia reported in clinical practice. Connexin 43 (Cx43) is a member of the connexin protein family, which serves important roles in signal transduction in vivo. The aim of the present study was to investigate the role of Cx43 in the induction and maintenance of atrial fibrillation by using an animal model of sympathomimetic atrial fibrillation. Cx43 was successfully knocked down in the myocardium with gene‑specific small interfering (si)RNA via lentiviral infection. A total of 25 dogs were randomly and evenly divided into five groups: Normal (N), rapid atrial pacing (RAP), isoproterenol (ISO) + RAP, RAP + Cx43 siRNA and ISO + RAP + Cx43 siRNA. The mRNA and protein levels, as well as the distribution of Cx43 on the cell membrane, were gradually decreased in each group compared with the N group following treatment (P<0.05). The induction rate of the atrial effective refractory period was not significantly affected in the RAP and RAP + Cx43 siRNA groups, whereas it was significantly reduced in the ISO + RAP and ISO + RAP + Cx43 siRNA groups compared with the N group (P<0.05). The induction rate of AF was gradually increased in the RAP + Cx43 siRNA, ISO + RAP and ISO + RAP + Cx43 siRNA groups compared with the N group (P<0.05). The expression of nerve growth factor (NGF) and tyrosine hydroxylase (TH) was gradually increased in the ISO + RAP and ISO + RAP + Cx43 siRNA groups compared with their respective controls (RAP and RAP + Cx43 siRNA groups, respectively). However, no significant difference in the levels of NGF and TH was observed between the RAP, RAP + Cx43 siRNA, ISO + RAP and ISO + RAP + Cx43 siRNA groups. The mitochondrial morphology in each group was notably altered compared with the N group. The mitochondrial reactive oxygen species production and apoptotic index were gradually increased in each group compared with the N group (P<0.05). The results of the present study suggest that Cx43 reduces susceptibility to AF. Downregulation of Cx43 mediates the induction and maintenance of sympathetic AF.
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http://dx.doi.org/10.3892/ijmm.2018.3648DOI Listing
August 2018

Push Force Analysis of Anchor Block of the Oil and Gas Pipeline in a Single-Slope Tunnel Based on the Energy Balance Method.

PLoS One 2016 10;11(3):e0150964. Epub 2016 Mar 10.

College of Pipeline and Civil Engineering, China University of Petroleum, Qingdao 266580, China.

In this paper, a single-slope tunnel pipeline was analysed considering the effects of vertical earth pressure, horizontal soil pressure, inner pressure, thermal expansion force and pipeline-soil friction. The concept of stagnation point for the pipeline was proposed. Considering the deformation compatibility condition of the pipeline elbow, the push force of anchor blocks of a single-slope tunnel pipeline was derived based on an energy method. Then, the theoretical formula for this force is thus generated. Using the analytical equation, the push force of the anchor block of an X80 large-diameter pipeline from the West-East Gas Transmission Project was determined. Meanwhile, to verify the results of the analytical method, and the finite element method, four categories of finite element codes were introduced to calculate the push force, including CAESARII, ANSYS, AutoPIPE and ALGOR. The results show that the analytical results agree well with the numerical results, and the maximum relative error is only 4.1%. Therefore, the results obtained with the analytical method can satisfy engineering requirements.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0150964PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4786285PMC
August 2016

Repurposing CRISPR/Cas9 for in situ functional assays.

Genes Dev 2013 Dec;27(23):2602-14

Department of Biochemistry, McGill University, Montreal, Quebec H3G 1Y6 Canada,;

RNAi combined with next-generation sequencing has proven to be a powerful and cost-effective genetic screening platform in mammalian cells. Still, this technology has its limitations and is incompatible with in situ mutagenesis screens on a genome-wide scale. Using p53 as a proof-of-principle target, we readapted the CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR associated 9) genome-editing system to demonstrate the feasibility of this methodology for targeted gene disruption positive selection assays. By using novel "all-in-one" lentiviral and retroviral delivery vectors heterologously expressing both a codon-optimized Cas9 and its synthetic guide RNA (sgRNA), we show robust selection for the CRISPR-modified Trp53 locus following drug treatment. Furthermore, by linking Cas9 expression to GFP fluorescence, we use an "all-in-one" system to track disrupted Trp53 in chemoresistant lymphomas in the Eμ-myc mouse model. Deep sequencing analysis of the tumor-derived endogenous Cas9-modified Trp53 locus revealed a wide spectrum of mutants that were enriched with seemingly limited off-target effects. Taken together, these results establish Cas9 genome editing as a powerful and practical approach for positive in situ genetic screens.
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http://dx.doi.org/10.1101/gad.227132.113DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3861673PMC
December 2013

RNA-level unscrambling of fragmented genes in Diplonema mitochondria.

RNA Biol 2013 Feb 16;10(2):301-13. Epub 2013 Jan 16.

Department of Biochemistry, Université de Montréal, Montreal, QC, Canada.

We previously reported a unique genome with systematically fragmented genes and gene pieces dispersed across numerous circular chromosomes, occurring in mitochondria of diplonemids. Genes are split into up to 12 short fragments (modules), which are separately transcribed and joined in a way that differs from known trans-splicing. Further, cox1 mRNA includes six non-encoded uridines indicating RNA editing. In the absence of recognizable cis-elements, we postulated that trans-splicing and RNA editing are directed by trans-acting molecules. Here, we provide insight into the post-transcriptional processes by investigating transcription, RNA processing, trans-splicing and RNA editing in cox1 and at a newly discovered site in cob. We show that module precursor transcripts are up to several thousand nt long and processed accurately at their 5' and 3' termini to yield the short coding-only regions. Processing at 5' and 3' ends occurs independently, and a processed terminus engages in trans-splicing even if the module's other terminus is yet unprocessed. Moreover, only cognate module transcripts join, though without directionality. In contrast, module transcripts requiring RNA editing only trans-splice when editing is completed. Finally, experimental and computational analyses suggest the existence of RNA trans-factors with the potential for guiding both trans-splicing and RNA editing.
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http://dx.doi.org/10.4161/rna.23340DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3594288PMC
February 2013

Group I-intron trans-splicing and mRNA editing in the mitochondria of placozoan animals.

Trends Genet 2009 Sep 27;25(9):381-6. Epub 2009 Aug 27.

Robert Cedergren Centre for Bioinformatics and Genomics, Département de Biochimie, Université de Montréal, C.P. 6128, Montréal, Québec, H3T 1J4, Canada.

Placozoa - the simplest known free-living animals - have been considered primitive, early diverging metazoans based on mitochondrial genome structure and phylogeny. Here we reanalyze placozoan mitochondrial DNAs, reported to include a highly unorthodox, fragmented and incomplete cox1 gene. We discover overlooked exons and split group I introns that mediate trans-splicing of the discontinuous placozoan cox1. Furthermore, we find that cox1 expression involves U-to-C editing, reconstituting an otherwise invariant, essential histidine involved in copper binding. These atypical features qualify placozoan mitochondrial gene and genome organization as derived rather than primitive. Whether the Placozoa diverged early or late during metazoan evolution remains unresolved by mitochondrial phylogeny.
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http://dx.doi.org/10.1016/j.tig.2009.07.003DOI Listing
September 2009

Blocking UV-induced eIF2alpha phosphorylation with small molecule inhibitors of GCN2.

Chem Biol Drug Des 2009 Jul;74(1):57-67

Department of Biochemistry, McGill University, Montreal, Quebec, Canada.

The eIF2alpha kinase general control non-depressible 2 integrates translation initiation rates to amino acid availability. General control non-depressible 2 also regulates translation initiation during synaptic plasticity and GCN2(-/-) mice show improved memory compared with wild-type mice with a reduced threshold for triggering late long-term potentiation. This property suggests that inhibiting general control non-depressible 2 function might represent a therapeutic avenue for improving memory. We screened for general control non-depressible 2 inhibitors using a small library of known kinase inhibitors and ATP-analogs and identified three compounds--indirubin-3'-monoxime, SP600125 and a SyK inhibitor with activity against general control non-depressible 2. All three compounds inhibit the ability of general control non-depressible 2 to phosphorylate eIF2alphain vitro as well as in vivo following UV-treatment of mouse embryonic fibroblasts. Using computer-assisted modeling, we modeled the binding of the inhibitors in the ATP-binding site of general control non-depressible 2. This work provides the molecular basis for undertaking structure-activity relationships of these compounds in order to develop specific inhibitors of general control non-depressible 2.
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http://dx.doi.org/10.1111/j.1747-0285.2009.00827.xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8221234PMC
July 2009

Homogenous time resolved fluorescence assay to identify modulators of cap-dependent translation initiation.

Comb Chem High Throughput Screen 2007 Mar;10(3):181-8

Department of Biochemistry, McIntyre Medical Sciences Building, 3655 Promenade Sir William Osler, McGill University, Montreal, Quebec, Canada, H3G 1Y6.

Eukaryotic initiation factor (eIF) 4F plays a key role in recruiting 40S ribosomes and associated factors to mRNA templates during translation initiation. The function of this heterotrimeric complex is to deliver an RNA helicase to the 5' cap proximal region of mRNAs in preparation for ribosome binding. To study the interaction between subunits of this complex, as well as identify small molecules that could interfere with their association, we developed a time resolved fluorescence assay that allows monitoring of interactions between two subunits of eIF4F. We have performed a small molecule chemical screen of >73,000 compounds using this assay.
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http://dx.doi.org/10.2174/138620707780126688DOI Listing
March 2007

Ribavirin is not a functional mimic of the 7-methyl guanosine mRNA cap.

RNA 2005 Aug;11(8):1238-44

Department of Biochemistry, McIntyre Medical Sciences Building, McGill University, Montreal, Québec H3G 1Y6, Canada.

Ribavirin is a guanosine ribonucleoside analog that displays broad-spectrum anti-viral activity and is currently used for the treatment of some viral infections. Ribavirin has recently been proposed to also be a mimic of the 7-methyl guanosine cap found at the 5' end of mRNAs. To obtain supporting functional data for this hypothesis, we assessed the ability of ribavirin triphosphate to interfere with the interaction between eIF4E and 7-methyl guanosine capped mRNA. In chemical cross-linking assays, cap-affinity chromatography, and cap-dependent translation assays, ribavirin was unable to function as a cap analog.
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http://dx.doi.org/10.1261/rna.2930805DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1370807PMC
August 2005

Regulation of cisplatin resistance and homologous recombinational repair by the TFIIH subunit XPD.

Cancer Res 2002 Oct;62(19):5457-62

Lady Davis Institute for Medical Research, Sir Mortimer B. Davis-Jewish General Hospital, Quebec, Canada H3T 1E2.

We have recently completed screening of the National Cancer Institute human tumor cell line panel and demonstrated that among four nucleotide excision repair proteins (XPA, XPB, XPD, and ERCC1), only the TFIIH subunit XPD endogenous protein levels correlate with alkylating agent drug resistance. In the present study, we extended this work by investigating the biological consequences of XPD overexpression in the human glioma cell line SK-MG-4. Our results indicate that XPD overexpression in SK-MG-4 cells leads to cisplatin resistance without affecting the nucleotide excision repair activity or UV light sensitivity of the cell. In contrast, in SK-MG-4 cells treated with cisplatin, XPD overexpression leads to increased Rad51-related homologous recombinational repair, increased sister chromatid exchanges, and accelerated interstrand cross-link removal. Moreover, we present biochemical evidence of an XPD-Rad51 protein interaction, which is modulated by DNA damage. To our knowledge, this is the first description of functional cross-talk between XPD and Rad51, which leads to bifunctional alkylating agent drug resistance and accelerated removal of interstrand cross-links.
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October 2002
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