Publications by authors named "Yifang Yuan"

24 Publications

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Survey on sodium and potassium intake in patients with hypertension in China.

J Clin Hypertens (Greenwich) 2021 Sep 25. Epub 2021 Sep 25.

Shanghai Institute of Hypertension, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

Sodium and potassium intake in hypertensive patients in China is not clear. The authors aimed to investigate the distribution of sodium and potassium intake in hypertensive patients in China, and to analyze the relationship between sodium and potassium intake and blood pressure. The study was performed in 130 hospitals from 23 provinces across China from 2016 to 2019. Finally, 9501 hypertensive patients average aged 54 years were included. 24 h urinary sodium and potassium excretion were measured. Distribution of urinary electrolytes were described according to age, gender and region. The association between urinary electrolytes and blood pressure was analyzed by multivariate linear regression. Hypertensive patients exhibited an average 24 h urinary sodium and potassium excretion of 156.7 ± 81.5 mmol/d and 39.2 ± 20.2 mmol/d (equivalent to sodium chloride of 9.2 g/d, potassium chloride of 2.9 g/d), sodium/potassium ratio (median) of 4.14 (2.92,5.73). Urinary electrolytes were lower in women than men (sodium: 171.1 vs 138.7, p < .05; potassium: 40.3 vs 37.7, p < .05), in the elderly than in the younger (sodium: 168.7 vs 139.9, p < .05; potassium: 39.5 vs. 37.5, p < .05). For every 1 unit of Na/K ratio increase, blood pressure increased by 0.46/0.24 mmHg. Blood pressure was 2.75/1.27 mmHg higher in quartile 4 than quartile 1 of Na/K. It remains high sodium and low potassium for hypertensive patients in China. Decreased sodium, Na/K ratio and increased potassium may help for blood pressure management.
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http://dx.doi.org/10.1111/jch.14355DOI Listing
September 2021

Long noncoding RNA ANCR promotes migration, invasion, EMT progress and stemness of nasopharyngeal carcinoma cells via the miR-4731-5p/NMT1 axis.

Pathol Res Pract 2021 Aug 3;224:153540. Epub 2021 Jul 3.

Department of Otolaryngology-Head and Neck Surgery, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 201999, China; Ear Institute Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai 200025, China. Electronic address:

Background: In our previous study, we revealed that Antidifferentiation noncoding RNA (ANCR) promoted proliferation and radiation resistance of nasopharyngeal carcinoma (NPC) cells. However, the molecular mechanism and function of ANCR are not fully studied. The current study aimed to further investigate the role and underlying molecular mechanism of ANCR in NPC.

Methods: RT-qPCR and western blot analyses were used to detect the levels of RNAs and proteins in NPC cells. Wound healing and Transwell assays were used to examine the migration and invasion of NPC cells. The relationship among ANCR, miR-4731-5p and N-myristoyltransferase 1 (NMT1) was investigated by RIP and luciferase reporter assays. The NPC cell stemness was accessed by the sphere formation assay.

Results: ANCR was significantly highly expressed in NPC cell lines. Silenced ANCR suppressed cell migration, invasion epithelial-mesenchymal transition (EMT) process and cell stemness in NPC. Furthermore, ANCR sponged miR-4731-5p to upregulate the NMT1 expression. Rescue assays indicated that NMT1 neutralized the antioncogenic effect induced by silenced ANCR on NPC cells.

Conclusions: Long noncoding RNA ANCR suppresses malignant behaviors of nasopharyngeal carcinoma cells by regulating miR-4731-5p/NMT1 axis.
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http://dx.doi.org/10.1016/j.prp.2021.153540DOI Listing
August 2021

The association between ECG criteria and Echo criteria for left ventricular hypertrophy in a general Chinese population.

Ann Noninvasive Electrocardiol 2021 Sep 26;26(5):e12880. Epub 2021 Jul 26.

Department of Cardiology, Tsinghua University, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Beijing, China.

Background: Several ECG criteria have been widely used for diagnosis of left ventricular hypertrophy (LVH) in clinical practice. However, their performance in a general Chinese population is limited.

Methods And Results: A multi-stage, stratified cluster sampling across China was performed and 7415 representative Chinese adults aged 18-85 years were analyzed. ECG was collected by using GE MAC 5500 machine. The association between five ECG-LVH criteria (i.e., Peguero-Lo Presti, Cornell, Cornell product, Sokolow-Lyon and Sokolow-Lyon product) and echocardiographic LVH (Echo-LVH) was assessed by Pearson's correlation, diagnostic statistics like predictive values, and receiver operating characteristics (ROC) curve. We found that the prevalence of the Echo-LVH was 11% while ECG-LVH ranged from 3% to 27%. All ECG-LVH criteria had high negative predictive value (NPV) (89%) and specificity (73-96%) but low positive predictive value (PPV) (12-24%) and sensitivity (4-29%). The newly Peguero-Lo Presti criteria had higher sensitivity (29%) but lower specificity (73%) and accuracy (68%) compared with other criteria. Cornell product had the best diagnostic performance (AUC: 0.59), as well as the highest specificity (96%) and accuracy (86%) but lowest sensitivity (4%). Among single-lead components of ECG criteria, R voltage and QRS duration performed relatively better than others. Hypertensive and older individuals had higher sensitivity but lower specificity and accuracy than their counterparts.

Conclusion: ECG-LVH criteria had high NPV to detect Echo-LVH. Though with higher sensitivity, Peguero-Lo Presti criteria did not have better diagnostic performance to detect Echo-LVH. R and QRS duration had stronger association with Echo-LVH among all single-lead components.
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http://dx.doi.org/10.1111/anec.12880DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8411759PMC
September 2021

Sciatic Herpes Zoster Suspected of Lumbar Disc Herniation: An Infrequent Case Report and Literature Review.

Front Surg 2021 7;8:663740. Epub 2021 May 7.

Department of Orthopeadics, Tangdu Hospital, Fourth Military Medical University, Xi'an, China.

The symptoms of sciatic herpes zoster are sometimes difficult to distinguish from sciatica caused by lumbar disc herniation. We describe a case of suspected lumbar disc herniation with sciatic herpes zoster to reduce the rate of misdiagnosis. A 55-year old man, male, developed low back pain after carrying heavy items 20 years ago. Characteristics of symptoms: 1. Symptoms were aggravated in the upright lumbar forward flexion position; 2. The VAS (leg) score was 8-9 points and the VSA (lumbar) score was 0 point; 3. It can be relieved when rested in the supine position; 4. It came on intermittently with radiation pain in the right lower limb. There were several attacks every year. One month ago, there was radiating pain in the right lower limb. The pain was from the back of the right hip, behind the thigh, in lateral crural region, to the back of the foot. And Symptoms worsened for 10 days. The VAS score was 8 points. Pain could not be relieved by rest or changing posture. There was no back pain, no lower limbs, weak walking, no claudication and other symptoms. Analgesics and neurotrophic drugs are ineffective. After the application of antiviral drugs, the radiation pain in the right lower extremity was significantly relieved. We describe this case in detail and discuss how to make an authentic diagnosis, with a concomitant literature review.
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http://dx.doi.org/10.3389/fsurg.2021.663740DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138428PMC
May 2021

Enriched Au nanoclusters with mesoporous silica nanoparticles for improved fluorescence/computed tomography dual-modal imaging.

Cell Prolif 2021 Apr 25;54(4):e13008. Epub 2021 Feb 25.

Department of Stomatology, Chinese PLA General Hospital, Beijing, China.

Objectives: Au nanoclusters (AuNCs) have been used widely in fluorescence bio-imaging because of their good fluorescence, small particle size and non-cytotoxicity. AuNCs are also efficient in computed tomography (CT) imaging. Hence, a dual-modal imaging probe can be constructed without any complicated modification processes by exploiting the excellent performance of AuNCs. In the present study, AuNCs were enriched with mesoporous silica nanoparticles (MSNs) to obtain enhanced fluorescence/CT dual-modal imaging, which was capable of acquiring more imaging information for diseases compared with single-mode imaging.

Materials And Methods: Biocompatible bovine serum albumin (BSA)-capped AuNCs were prepared and loaded into amine-functionalized MSNs to form [email protected] BSA-AuNCs, MSNs, and [email protected] were characterized by ultraviolet-visible (UV-vis) spectra, transmission electron microscopy (TEM), fluorescence spectra, and zeta potential. CT imaging was recorded using micro-CT scanning. Fluorescence imaging was measured using confocal laser scanning microscopy and flow cytometry.

Results: The prepared AuNCs and MSNs possessed good properties as previously reported. The fluorescence intensity and CT value of the AuNCs were enhanced after being enriched with MSNs. The nanoparticles were both non-cytotoxic. Confocal laser scanning microscopy and flow cytometry indicated that [email protected] in CAL-27 cells showed improved fluorescence imaging compared with simple AuNCs at the same concentration.

Conclusions: The results revealed that the strategy of enriching AuNCs with MSNs can obtain highly sensitive fluorescence/CT dual-modal imaging, which indicated the potential of this nanoparticle in the diagnosis and treatment of disease.
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http://dx.doi.org/10.1111/cpr.13008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8016642PMC
April 2021

A Method for Estimating 24-Hour Urinary Sodium Excretion by Casual Urine Specimen in Chinese Hypertensive Patients.

Am J Hypertens 2021 08;34(7):718-728

Institute of Hypertension, People's Hospital, Peking University, Beijing, China.

Background: High salt intake is a known risk factor of hypertension, which in turn increases the risk of stroke and cardiovascular diseases. The aim of this study was to develop and evaluate a method for predicting 24-hour urinary sodium excretion (UNa24h) using casual urine specimens in Chinese hypertensive patients.

Methods: A total of 966 patients with hypertension were included from 8 provinces across China. A UNa24h prediction model (Sun_C method) was developed for males and females using linear regression based on age, weight, sodium concentration in the spot urine (UNaspot), and creatinine concentration in the spot urine (UCrspot). The data were split into the training (70%) and testing (30%) sets to, respectively, develop and evaluate the Sun_C method.

Results: Compared with the Kawasaki, INTERSALT, and Tanaka methods, Sun_C method achieved a low and consistent mean bias (1.1 mmol/d) within the range from 106 to 212 mmol/d of UNa24h (equivalent to NaCl intake of 6-12 g/d). In addition, the Sun_C method showed no significant difference between the measured and estimated UNa24h in a paired t-test (P = 0.689). At individual level, Sun_C method had 79.8% of individuals at the cutoff under ±30% level.

Conclusions: Sun_C method may prove a reasonable method to estimate the daily dietary sodium intakes (particularly in the range of 6-12 g/d of NaCl) in Chinese hypertensive patients using spot urine measurements. As the amount of data increases in the future, the performance of our formulae will be further improved.
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http://dx.doi.org/10.1093/ajh/hpab020DOI Listing
August 2021

Comparison of postoperative outcomes between patients with positive and negative straight leg raising tests who underwent full-endoscopic transforaminal lumbar discectomy.

Sci Rep 2020 10 5;10(1):16516. Epub 2020 Oct 5.

Department of Orthopedics, Tangdu Hospital, The Fourth Military Medical University, 569 Xinsi Road, Xi'an, 710038, Shaanxi, People's Republic of China.

Full-endoscopic transforaminal lumbar discectomy (FETD) is increasingly used in patients with lumbar disc herniation (LDH). There is little knowledge on the related factors, including the straight leg raising test (SLR), that influence the operation. Consecutive patients with LDH who came to our hospital from August 2015 to September 2016 and underwent FETD surgery were included. Four kinds of scores, including the VAS (lumbar/leg), ODI and JOA values, were measured and reassessed after FETD to assess the surgical outcomes. There was a statistically significant difference between the scores before surgery and at each postoperative follow-up. In addition, the increase in the JOA score postoperatively was statistically significant compared with that before surgery. There were statistically significant differences among the three subpopulations [patients considered SLR positive (0°-30°), SLR positive (31°-60°) and SLR negative (61°-)] in the changes in the VAS (leg), ODI and JOA values. However, there were no statistically significant differences among the three subpopulations [patients considered SLR positive (0°-30°), SLR positive (31°-60°) and SLR negative (61°-)] in the changes in VAS score (lumbar). FETD showed great effectiveness in treating patients with lumbar disc herniation. Patients who were SLR negative may receive greater benefit from FETD.
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http://dx.doi.org/10.1038/s41598-020-73357-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7536236PMC
October 2020

CURB-65 may serve as a useful prognostic marker in COVID-19 patients within Wuhan, China: a retrospective cohort study.

Epidemiol Infect 2020 10 1;148:e241. Epub 2020 Oct 1.

Department of Cardiology, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing102218, China.

A recently developed pneumonia caused by SARS-CoV-2 has quickly spread across the world. Unfortunately, a simplified risk score that could easily be used in primary care or general practice settings has not been developed. The objective of this study is to identify a simplified risk score that could easily be used to quickly triage severe COVID-19 patients. All severe and critical adult patients with laboratory-confirmed COVID-19 on the West campus of Union Hospital, Wuhan, China, from 28 January 2020 to 29 February 2020 were included in this study. Clinical data and laboratory results were obtained. CURB-65 pneumonia score was calculated. Univariate logistic regressions were applied to explore risk factors associated with in-hospital death. We used the receiver operating characteristic curve and multivariate COX-PH model to analyse risk factors for in-hospital death. A total of 74 patients (31 died, 43 survived) were finally included in the study. We observed that compared with survivors, non-survivors were older and illustrated higher respiratory rate, neutrophil-to-lymphocyte ratio, D-dimer and lactate dehydrogenase (LDH), but lower SpO2 as well as impaired liver function, especially synthesis function. CURB-65 showed good performance for predicting in-hospital death (area under curve 0.81, 95% confidence interval (CI) 0.71-0.91). CURB-65 ⩾ 2 may serve as a cut-off value for prediction of in-hospital death in severe patients with COVID-19 (sensitivity 68%, specificity 81%, F1 score 0.7). CURB-65 (hazard ratio (HR) 1.61; 95% CI 1.05-2.46), LDH (HR 1.003; 95% CI 1.001-1.004) and albumin (HR 0.9; 95% CI 0.81-1) were risk factors for in-hospital death in severe patients with COVID-19. Our study indicates CURB-65 may serve as a useful prognostic marker in COVID-19 patients, which could be used to quickly triage severe patients in primary care or general practice settings.
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http://dx.doi.org/10.1017/S0950268820002368DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7573457PMC
October 2020

Downregulated brain and muscle aryl hydrocarbon receptor nuclear translocator-like protein-1 inhibits osteogenesis of BMSCs through p53 in type 2 diabetes mellitus.

Biol Open 2020 07 8;9(7). Epub 2020 Jul 8.

Department of Stomatology, Chinese PLA General Hospital, Beijing 100853, China

The bone marrow mesenchymal stem cells (BMSCs)-mediated abnormal bone metabolism can delay and impair the bone remodeling process in type 2 diabetes mellitus (T2DM). Our previous study demonstrated that the downregulation of brain and muscle aryl hydrocarbon receptor nuclear translocator-like protein 1 (BMAL1), a circadian clock protein, inhibited the Wnt/β-catenin pathway via enhanced GSK-3β in diabetic BMSCs. In this article, we confirmed that the downregulated BMAL1 in T2DM played an inhibitory role in osteogenic differentiation of BMSCs. Upregulation of BMAL1 in the diabetic BMSCs significantly recovered the expression pattern of osteogenic marker genes and alkaline phosphatase (Alp) activity. We also observed an activation of the p53 signaling pathways, exhibited by increased p53 and p21 in diabetic BMSCs. Downregulation of p53 resulting from overexpression of BMAL1 was detected, and when we applied p53 gene silencing (shRNA) and the p53 inhibitor, pifithrin-α (PFT-α), the impaired osteogenic differentiation ability of diabetic BMSCs was greatly restored. However, there was no change in the level of expression of BMAL1. Taken together, our results first revealed that BMAL1 regulated osteogenesis of BMSCs through p53 in T2DM, providing a novel direction for further exploration of the mechanism underlying osteoporosis in diabetes.
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http://dx.doi.org/10.1242/bio.051482DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7358138PMC
July 2020

Anti-allergic function of α-Tocopherol is mediated by suppression of PI3K-PKB activity in mast cells in mouse model of allergic rhinitis.

Allergol Immunopathol (Madr) 2020 Jul - Aug;48(4):395-400. Epub 2020 Apr 23.

Department of Otolaryngology, Affiliated ZhangJiaGang Hospital of Soochow University, Suzhou, 215600, China.

Background: Alpha-Tocopherol (α-TCP), one major form of vitamin E, has been known as a treatment for airway allergic inflammation. However, the role and mechanism of α-TCP in treating allergic rhinitis remains unclear.

Objective: In this study we examined the inhibitory function of α-TCP in a mouse model of allergic rhinitis.

Methods: Allergic phenotype was examined by hematoxylin and eosin staining. Total IgE, OVA-specific IgE, OVA-specific IgG1 and OVA-specific IgG2a levels were examined by ELISA. mRNA expression was measured by qPCR, protein levels were examined by Western Blot.

Results: Histological analysis of the nasal membranes revealed that there was a significant reduction in inflammatory cells appearance in cross-sections in alpha-TCP treatment of Ovalbumin (OVA)-sensitized mice compared to OVA sensitized animals. In addition, eosinophils were significantly reduced in nasal mucosa of alpha-TCP treatment of OVA-sensitized mice compared to the OVA group. Lower total IgE, OVA-specific IgE, OVA-specific IgG1 and OVA-specific IgG2a levels were found in alpha-TCP treatment of OVA-sensitized mice compared to the OVA group. Furthermore, we found that the subepithelial distribution of tryptase positive mast cells was reduced in the alpha-TCP treatment of OVA-sensitized mice. More importantly, the PI3K-PKB pathway was suppressed by α-TCP in mast cells.

Conclusions: Our results demonstrated that α-TCP-mediated suppression of PI3K-PKB activity in mast cells is a potential mechanism of anti-allergic function of α-TCP.
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http://dx.doi.org/10.1016/j.aller.2019.11.005DOI Listing
April 2021

Effect of Biospray Dressings on Eosinophil Infiltration in the Nasal Mucosa and Serum IgE Levels After Nasal Provocation in Experimental Allergic Rhinitis.

Allergy Rhinol (Providence) 2020 Jan-Dec;11:2152656720902142. Epub 2020 Mar 5.

Institute of Translational Medicine, The affiliated Zhangjiagang hospital of Soochow University, Suzhou, China.

Purpose: To investigate the effect of biospray dressing on the extent of eosinophil infiltration in the nasal mucosa and the level of serum IgE in experimental allergic rhinitis with nasal provocation.

Method: Twenty-four BALB/c mice were randomly divided into the normal control group, allergic rhinitis (AR) group, dexamethasone (DEX) treatment group, and biospray dressing (BD) group. The mice in the latter 3 groups were prepared for animal models of AR according to standard protocols. Mice in the BD group were administered a nasal spray before the nasal provocation, and those in the DEX group were administered an intraperitoneal injection of DEX. The nasal mucosa and serum were collected from each group. Nasal mucosa eosinophil infiltration was evaluated using hematoxylin and eosin staining, and enzyme-linked immunosorbent assay (ELISA) was performed to analyze the serum IgE expression.

Results: Eosinophil infiltration (AR vs BD  = .009) in the nasal mucosa and serum IgE expression (AR vs BD  = .001) were significantly lower in the BD group than in the AR group. There were no significant differences in the extent of eosinophil infiltration in the nasal mucosa or serum IgE expression between the BD and DEX groups.

Conclusion: Biospray dressings can significantly reduce allergen provocation in the nasal cavity and have a protective effect on the nasal mucosa. They can be used for the prevention and treatment of AR.
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http://dx.doi.org/10.1177/2152656720902142DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7066584PMC
March 2020

Structural, vibrational and electrical properties of type-II Dirac semimetal PtSe under high pressure.

J Phys Condens Matter 2019 Oct 11;31(41):415402. Epub 2019 Jul 11.

Institutes of Physical Science and Information Technology, Anhui University, Hefei 230601, People's Republic of China. Key Laboratory of Structure and Functional Regulation of Hybrid Materials (Anhui University), Ministry of Education, Hefei 230601, People's Republic of China.

We present a high-pressure study of type-II Dirac semimetal PtSe single crystals through synchrotron x-ray diffraction (XRD), electrical transport and Raman scattering measurements in diamond anvil cells with pressures up to 36.1-42.3 GPa, from which two critical pressure points associated with unusual electron-phonon coupling are unraveled. We show that both resistance and phonon linewidth of Raman modes display anomalies at the first critical pressure of P ~ 10 GPa, in accordance with a scenario of pressure-induced disappearance/appearance of type-II/type-I Dirac points around P predicted previously. The second critical pressure P ~ 20 GPa may correspond to a structural crossover of PtSe from quasi-2D lattice to 3D network, which is revealed via detailed analysis of the structural parameters extracted from XRD refinement, Raman modes shifts as well as parameters from fitting of the low-temperature resistance. Our results demonstrate great tunability of PtSe via strain engineering, thanks to the single p-orbital manifold derived electronic states that are susceptible to out-of-plane and in-plane distances.
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http://dx.doi.org/10.1088/1361-648X/ab315eDOI Listing
October 2019

A randomized controlled trial with ≥5 years of follow-up comparing minimally invasive and open transforaminal lumbar interbody fusion in disc herniation at single level.

Exp Ther Med 2019 May 12;17(5):3614-3620. Epub 2019 Mar 12.

Department of Orthopedics, Tangdu Hospital, The Fourth Military Medical University, Xi'an, Shaanxi 710038, P.R. China.

Minimally invasive transforaminal lumbar interbody fusion (m-TLIF) using transpedicular screws has various advantages over classical open (c-) TLIF. Up to date, comparative analyses of the TLIF procedures were following patients for <5 years. The objective of the present study was to compare the clinical effectiveness and complications of m- and c-TLIF in patients with single-level disc herniation with ≥5 years follow-up. Between June 2008 to July 2010, 91 patients with single-level lumbar degeneration were recruited and were randomly divided into two groups: m-TLIF and c-TLIF. The analyzed outcome measurements included: Surgery duration, intraoperative blood loss, X-ray exposure time, T2 relaxation time in magnetic resonance imaging (MRI), visual analogue scale (VAS) scores, Japanese orthopedic association (JOA) scores, fusion rate and complications during follow-up. No significant differences between m- and c-TLIF were observed with respect to surgery duration (P=0.077), volume of blood loss (P=0.115), complications and the need for an additional surgery (P=0.632). Significant differences between the groups were observed for X-ray exposure time (P<0.001) and MRI T2 relaxation times at 3 months post-surgery (P<0.001). At day 7 post surgery, recorded VAS and JOA scores were significantly improved in the m-TLIF compared with the c-TLIF group and non-significant differences between the groups were observed at >1 month follow-up. m-TLIF was a safe and effective tool in treating single-level lumbar disc herniation. However, careful attention to the surgical technique and precise anatomical knowledge were required. Further studies and refinement of the surgical techniques are necessary prior to treating multiple or more extensive lesions using the m-TLIF method.
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http://dx.doi.org/10.3892/etm.2019.7368DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6447782PMC
May 2019

Implementation of Enterprise Imaging Strategy at a Chinese Tertiary Hospital.

J Digit Imaging 2018 08;31(4):534-542

Information Center, Xuanwu Hospital, Capital Medical University, No. 45 Changchun Street, Xicheng District, Beijing, 100053, People's Republic of China.

Medical images have become increasingly important in clinical practice and medical research, and the need to manage images at the hospital level has become urgent in China. To unify patient identification in examinations from different medical specialties, increase convenient access to medical images under authentication, and make medical images suitable for further artificial intelligence investigations, we implemented an enterprise imaging strategy by adopting an image integration platform as the main tool at Xuanwu Hospital. Workflow re-engineering and business system transformation was also performed to ensure the quality and content of the imaging data. More than 54 million medical images and approximately 1 million medical reports were integrated, and uniform patient identification, images, and report integration were made available to the medical staff and were accessible via a mobile application, which were achieved by implementing the enterprise imaging strategy. However, to integrate all medical images of different specialties at a hospital and ensure that the images and reports are qualified for data mining, some further policy and management measures are still needed.
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http://dx.doi.org/10.1007/s10278-017-0044-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6113152PMC
August 2018

Doxorubicin-loaded environmentally friendly carbon dots as a novel drug delivery system for nucleus targeted cancer therapy.

Colloids Surf B Biointerfaces 2017 Nov 10;159:349-359. Epub 2017 Jul 10.

Institution of Stomatology, The General Hospital of China LA, Beijing 100853, China. Electronic address:

Chemotherapy is widely applied against various kinds of carcinoma. Generally, chemotherapeutic agents, such as Doxorubicin (DOX), Paclitaxel (PTX), 5-Fluorouracil (5-FU), Methotrexate (MTX), and Vinblastine (VLB) are combined with a view to maximizing their efficacy. Unfortunately, chemotherapeutics are indiscriminate and also kill normal healthy cells, resulting in serious side effects. This non-productive and destructive distribution of chemotherapeutics is regarded as one of the largest problems associated with chemotherapy. Recently, the application of carbon dots (CDs) in cancer therapy has attracted considerable attention due to their attractive properties, such as biocompatibility and low toxicity. We report herein on the fabrication of CD-DOX antitumor drug complexes, from the combination of CDs and DOX, with a view to providing a novel and efficient strategy for cancer treatment. CDs were synthesized by hydrothermal treatment of milk, a simple and environmentally friendly synthetic process. DOX was conjugated to the CDs through electrostatic interactions via the multiple surface CD functional groups. The CD-DOX complexes exhibited pH-dependent DOX release behavior. A cytotoxicity study demonstrated that the CDs were non-cytotoxic in the range of concentrations used. Compared to free DOX, the CD-DOX complexes were significantly more destructive to the adenoid cystic carcinoma cell line (ACC-2), but exhibited lower toxicity to a mouse fibroblast cell line (L929). Confocal microscopy and flow cytometry confirmed that CD-DOX complexes increased cancer therapy efficiency through the localization of a much higher quantity of drugs in the nuclei of tumor cells and induced a higher rate of apoptosis in ACC-2 cells, compared to DOX alone.
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http://dx.doi.org/10.1016/j.colsurfb.2017.07.030DOI Listing
November 2017

Effects of Aging on the Proliferation and Differentiation Capacity of Human Periodontal Ligament Stem Cells.

Chin Med Sci J 2017 Jun;32(2):83-1

Department of Stomatology, Chinese PLA General Hospital, Beijing 100853, China.

Objective The aim of this study is to investigate the proliferation, differentiation and apoptosis of periodontal ligament stem cells (PDLSC) derived from different aged donors, and to evaluate the effects of aging on the biological characteristics of PDLSC.Methods Periodontal ligament tissues were obtained from 24 surgically extracted human premolars during orthodontics therapy. The specimens were divided into three groups according to the donor's age. Group A: 18-20 years, group B: 30-35 years, group C: 45-50 years. PDLSC were isolated and cultured using a tissue-block-based enzymolytic method by limiting dilution assay. The colony forming efficiency of PDLSC for three experimental groups was determined. Senescence-Associated β-Galactosidase (SA-β-G) expression in the three groups was examined using β-galactosidase staining working solution. Cell cycle and apoptosis of the PDLSC were examined by the flow cytometry. Alkaline phosphatase (ALP) activity was evaluated by ALP staining. The expression of osteoplastic differentiation related genes Runt-related transcription factor-2 (Runx-2), Collagen Type 1 (col-1), and ALP of PDLSC were examined by quantitative real-time RT-PCR.Results The colony forming efficiency of PDLSC in Group A, B and C was 36.67%, 22.67% and 9.33%, respectively, which decreased with donors' age (P<0.05). SA-β-G expression of the senescent PDLSC in group A, B and C were 4.14%, 16.39%, 50.38%, respectively (P<0.05). Cells in G2/S phase was 38.73%, 29.88%, 18.25% (P<0.05), and the apoptosis rate was 1.57%, 4.56%, 5.84% (P<0.05), in group A, B and C respectively. The ALP staining in the three groups decreased with the increase of donors' ages, and the expression of Runx-2, col-1 and ALP decreased gradually from group A to group C (all P<0.05), which indicated the osteogenic differentiation capacity of PDLSC decreased while donor aging.Conclusion Human PDLSC could be successfully isolated from periodontal ligament tissues of different aged donors. However, the proliferation and osteogenic differentiation capacity of PDLSC decreased while donor aging.
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http://dx.doi.org/10.24920/J1001-9294.2017.012DOI Listing
June 2017

γδ T cells are indispensable for interleukin-23-mediated protection against Concanavalin A-induced hepatitis in hepatitis B virus transgenic mice.

Immunology 2017 05 20;151(1):43-55. Epub 2017 Feb 20.

State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin, China.

Hepatitis B virus surface antigen (HBsAg) carriers are highly susceptible to liver injury triggered by environmental biochemical stimulation. Previously, we have reported an inverse correlation between γδ T cells and liver damage in patients with hepatitis B virus (HBV). However, whether γδ T cells play a role in regulating the hypersensitivity of HBsAg carriers to biochemical stimulation-induced hepatitis is unknown. In this study, using HBV transgenic (HBs-Tg) and HBs-Tg T-cell receptor-δ-deficient (TCR-δ ) mice, we found that mice genetically deficient in γδ T cells exhibited more severe liver damage upon Concanavalin A (Con A) treatment, as indicated by substantially higher serum alanine aminotransferase levels, further elevated interferon-γ (IFN-γ) levels and more extensive necrosis. γδ T-cell deficiency resulted in elevated IFN-γ in CD4 T cells but not in natural killer or natural killer T cells. The depletion of CD4 T cells and neutralization of IFN-γ reduced liver damage in HBs-Tg and HBs-Tg-TCR-δ mice to a similar extent. Further investigation revealed that HBs-Tg mice showed an enhanced interleukin-17 (IL-17) signature. The administration of exogenous IL-23 enhanced IL-17A production from Vγ4 γδ T cells and ameliorated liver damage in HBs-Tg mice, but not in HBs-Tg-TCR-δ mice. In summary, our results demonstrated that γδ T cells played a protective role in restraining Con A-induced hepatitis by inhibiting IFN-γ production from CD4 T cells and are indispensable for IL-23-mediated protection against Con A-induced hepatitis in HBs-Tg mice. These results provided a potential therapeutic approach for treating the hypersensitivity of HBV carriers to biochemical stimulation-induced liver damage.
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http://dx.doi.org/10.1111/imm.12712DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5382349PMC
May 2017

Interleukin-6-174G/C Polymorphism Contributes to Periodontitis Susceptibility: An Updated Meta-Analysis of 21 Case-Control Studies.

Dis Markers 2016 6;2016:9612421. Epub 2016 Dec 6.

Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.

. Chronic Periodontitis (CP) is suggested to be related to gene variations. Present study aims to quantitatively estimate the association between interleukin-6- (IL-6-) 174G/C polymorphism and CP susceptibility. . Pubmed, Embase, and Web of Science were searched up to May 2016. The meta-analyses were performed using STATA 12.0. . 21 studies were yielded. Significant associations were found under heterozygote comparison and dominant model in studies fulfilling HWE (GC versus GG: OR = 0.690, 95% CI = 0.560-0.849, = 0.000; CC + GC versus GG: OR = 0.690, 95% CI = 0.568-0.838, < 0.001); significant associations were found under heterozygote comparison and dominant model in Caucasian studies fulfilling HWE (GC versus GG: OR = 0.752, 95% CI = 0.577-0.980, = 0.035; CC + GC versus GG: OR = 0.737, 95% CI = 0.576-0.944, = 0.016); significant associations were found under allele comparison, heterozygote comparison, and dominant model in Brazilian population (C versus G: OR = 0.648, 95% CI = 0.497-0.845, = 0.001; GC versus GG: OR = 0.621, 95% CI = 0.441-0.876, = 0.007; CC + GC versus GG: OR = 0.649, 95% CI = 0.470-0.896, = 0.009). . IL-6 174 polymorphism is associated with CP susceptibility. In Brazilian and Caucasian population, IL-6 174 GG genotype plays as a risk factor to CP.
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http://dx.doi.org/10.1155/2016/9612421DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5168484PMC
February 2017

[Continuous positive airway pressure treatment for functional cardiac arrhythmias combined with obstructive sleep apnea hypopnea syndrome].

Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2015 Mar;50(3):221-4

Objective: To discuss the effect of continuous positive airway pressure (CPAP) treatment for functional cardiac arrhythmias combined with obstructive sleep apnea hypopnea syndrome.

Methods: Fifty-six OSAHS patients combined functional cardiac arrhythmia were randomized divided into two groups. The 28 patients in the control group were treated with metoprolol according to cardiac guidelines, the other 28 cases in the experimental group were treated with CPAP therapy combined with metoprolol. AHI and the lowest oxygen saturation (LSaO2) were tested before and after treatment.

Results: The efficiency rates were 57.1% and 17.9% in experimental and control group respectively, with statistical difference (χ2 = 7.62, P < 0.01). Total effective rates were 85.7% and 53.6% respectively, with statistical difference (χ2 = 5.41, P < 0.05). In the experimental group, there were three treatment subgroups. After treatment, AHI and the lowest oxygen saturation were significantly different (P < 0.05).

Conclusion: CPAP treatment can effectively eliminate respiratory disturbance index, improve the symptoms of hypooxygen at night, and effectively improve the therapeutic effect of functional cardiac arrhythmias in OSAHS patients combined with functional arrhythmia.
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March 2015

[Effect of Er:YAG laser on the dynamic changes in subgingival flora in type 2 diabetic patients with periodontitis].

Hua Xi Kou Qiang Yi Xue Za Zhi 2014 Apr;32(2):171-6

Objective: To observe the changes in subgingival microflora before and after Er:YAG laser treatment on diabetic patients with periodontitis, and to compare with the subgingival microflora of chronic periodontitis.

Methods: Subgingival plaque of 13 pairs of teeth (26 sites) was selected from type 2 diabetic patients at pretreatment, one month post-treatment, and three months post-treatment. Subgingival plaque was also obtained from 11 cases of moderate to severe chronic periodontitis with similar severity of periodontitis. The DNA of the subgingival plaque samples was extracted. Whole bacterial 16S rDNA gene fragments separated by denaturing gradient gel electrophoresis. Specific DNA bands were then chosen for retrieval and sequencing.

Results: The gene sequencing results of the special DNA bands of subgingival plaque samples show that the pathogenic bacteria of both diabetic periodontitis and simple chronic periodontitis were Prevotella intermedia and Tannerella forsythia, respectively. The composition of the subgingival microflora before and after laser treatment changed. Some DNA bands, including that of Tannerella forsythia, disappeared or weakened one month after treatment. A new strip appeared, which belonged to Actinomyces sp.

Conclusion: The profiles of the subgingival microflora changed after treatment, and one month was indicated as an important stage. Er:YAG laser may have an important function in delaying microflora recolonization.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7030808PMC
April 2014

Optimized design of fluorinated polyimide based interleaver.

Appl Opt 2003 Jul;42(20):4202-7

College of Optics and Electron Information Engineering, University of Shanghai for Science and Technology, Shanghai 200093.

Statistical optimization method for the design of a fluorinated polyimide wavelength division element for optical communication is proposed. The opitimized device is an interleaver element suitable for dividing over 40 wavelengths in the 1550 nm band. Optimization considers the inherent polarization dependence of fluorinated polyimide based on measurements of the dispersion characteristics and birefringence of fluorinated polyimide film. A 40-wavelength device is designed by use of the proposed technique for a working wavelength of 1550 nm and a wavelength interval of 0.8 nm. The device exhibited a 1-dB passband of 0.5 nm and a 3-dB passband of 0.8 nm, and output wavelength fluctuation due to polarization effects of less than 0.08 nm.
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http://dx.doi.org/10.1364/ao.42.004202DOI Listing
July 2003

Optimized design of polarization-independent and temperature-insensitive broadband optical waveguide coupler by use of fluorinated polyimide.

Appl Opt 2003 Jul;42(20):4196-201

College of Optics and Electron Information Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.

A statistically optimized design method suitable for a polariation-independent and temperature-insensitive broadband waveguide coupler is proposed. By use of this method, a fluorinated polyimide waveguide 3-dB waveguide coupler for 1490 to approximately 1610 nm application is designed by optimizing polarization and temperature fluctuation. The validity of the design is verified through simulation based on the three-dimensional beam propagation method (3D-BPM), which revealed a coupling ratio of 50 +/- 0.8% in a 120-nm bandwidth in the temperature range -10 to 40 degrees C for both orthogonal polarizations.
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http://dx.doi.org/10.1364/ao.42.004196DOI Listing
July 2003

Optimized design of temperature-insensitive optical waveguide coupler with 120-nm bandwidth using fluorinated polyimide.

Appl Opt 2003 Jan;42(2):271-4

College of Optics and Electronic Information Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.

Method for designing optimized temperature-insensitive optical waveguide couplers by use of fluorinated polyimide is presented. Based on measured temperature and dispersion characteristics of fluorinated polyimide, a 3-dB waveguide coupler with a 120-nm bandwidth with minimal temperature variance is designed and verified through simulation based on three-dimensional beam propagation. The coupling ratio of the theoretical device is 50 +/- 0.7% in the waveband 1490 to approximately 1610 nm and the temperature range -10 to approximately 40 degrees C.
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http://dx.doi.org/10.1364/ao.42.000271DOI Listing
January 2003
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