Publications by authors named "Yifan Zhang"

823 Publications

Facile Construction of Iron/Nickel Phosphide Nanocrystals Anchored on N-B-Doped Carbon-Based Composites with Advanced Catalytic Capacity for 4-Nitrophenol and Methylene Blue.

Int J Mol Sci 2022 Jul 29;23(15). Epub 2022 Jul 29.

Hubei Provincial Key Laboratory of Green Materials for Light Industry, Hubei University of Technology, Wuhan 430068, China.

The search for a simple and effective method to remove organic dyes and color intermediates that threaten human safety from the water environment is urgent. Herein, we report a simple method for constructing iron/nickel phosphide nanocrystals anchored on N-B-doped carbon-based composites, using steam-exploded poplar (SEP) and graphene oxide (GO) as a carrier. The stability and catalytic activity of N-B-NiFeP/SEP and GO were achieved by thermal conversion in a N atmosphere and modifying the Fe/Ni ratio in gel precursors. N-B-NiFeP/SEP was employed for the catalytic hydrogenation of 4-nitrophenol (4-NP) and methylene blue (MB), using sodium borohydride in aqueous media at room temperature. This showed much better catalytic performances in terms of reaction rate constant (0.016 S and 0.041 S, respectively) and the activity factor, K (1.6 S·g and 8.2 S·g, respectively) compared to the GO carrier (0.0053 S and 0.035 S for 4-NP and MB, respectively). The strong interaction between the carrier's morphology and structure, and the vertically grown bimetallic phosphide nanoclusters on its surface, enhances charge transfer, electron transfer kinetics at the interface and Ni-Fe phosphide dispersion on the nanoclusters, and prevents dissolution of the nanoparticles during catalysis, thereby improving stability and achieving catalysis durability. These findings provide a green and simple route to efficient catalyst preparation and provide guidance for the rational selection of catalyst carriers.
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http://dx.doi.org/10.3390/ijms23158408DOI Listing
July 2022

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J Am Chem Soc 2022 Aug 11. Epub 2022 Aug 11.

Key Laboratory of Photochemistry, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

Fabrication of uniform two-dimensional (2D) structures from small molecules remains a formidable challenge for living self-assembly despite its great success in producing uniform one-dimensional (1D) structures. Here, we report the construction of unprecedented uniform 2D platelets with tailorable shapes and controlled sizes by creating new nuclei from a donor-acceptor (D-A) molecule and 1-hexanol to initiate 2D living self-assembly. We demonstrate that the D-A molecule undergoes 1-hexanol-induced twisting to form continuous alternative hydrogen bonds in-between under electrostatic attraction, which in turn forms a new nucleus. This connection architecture of the new nucleus allows to simultaneously regulate the growth rate of in two dimensions to generate 2D platelets of distinct shapes through simply varying the amount of 1-hexanol relative to hexane. Furthermore, the living nature of the new nucleus enables seeded growth of complex concentric multiblock 2D heteroplatelets by sequential and alternative addition of different D-A molecules. Interestingly, the resulting 2D platelets obtained by such living self-assembly exhibit enhanced photostability compared to those obtained by conventional self-assembly without the involvement of 1-hexanol.
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http://dx.doi.org/10.1021/jacs.2c07480DOI Listing
August 2022

A rotation invariant template matching algorithm based on Sub-NCC.

Math Biosci Eng 2022 Jun;19(9):9505-9519

School of Mechanical and Electrical Engineering, Guangzhou University, Guangzhou 511400, China.

This paper proposes an anti-rotation template matching method based on a portion of the whole pixels. To solve the problem that the speed of the original template matching method based on NCC (Normalized cross correlation) is too slow for the rotated image, a template matching method based on Sub-NCC is proposed, which improves the anti-jamming ability of the algorithm. At the same time, in order to improve the matching speed, the rotation invariant edge points are selected from the rotation invariant pixels, and the selected points are used for rough matching to quickly screen out the unmatched areas. The theoretical analysis and experimental results show that the accuracy of this method is more than 95%. For the search map at any angle with the resolution at the level of 300,000 pixel, after selecting the appropriate pyramid series and threshold, the matching time can be controlled to within 0.1 s.
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http://dx.doi.org/10.3934/mbe.2022442DOI Listing
June 2022

Association of ambient fine particulate matter exposure with gestational diabetes mellitus and blood glucose levels during pregnancy.

Environ Res 2022 Aug 2:114008. Epub 2022 Aug 2.

Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan, Shanxi, China. Electronic address:

Background: Previous studies have examined the associations between ambient fine particulate matter (PM) exposure and gestational diabetes mellitus (GDM). However, limited studies explored the relationships between PM exposure and blood glucose levels during pregnancy, especially in highly polluted areas.

Objectives: To examine the associations of prenatal ambient PM exposure with GDM and blood glucose levels, and to identify the sensitive exposure windows in a highly air-polluted area.

Methods: From July 2016 to October 2017, a birth cohort study was conducted in Beijing, China. Participants were interviewed in each trimester regarding demographics, lifestyle, living and working environment, and medical conditions. Participant's daily ambient PM levels from 3 m before last menstrual period (LMP) to the third trimester was estimated by a hybrid spatiotemporal model. Indoor air quality index was calculated based on environmental tobacco smoke, ventilation, cooking, painting, pesticide, and herbicide use. Distributed lag non-linear model was applied to explore the sensitive weeks of PM exposure.

Results: Of 165 pregnant women, 23 (13.94%) developed GDM. After adjusting for potential confounders, PM exposure during the 1st trimester was associated with higher odds of GDM (10 μg/m increase: OR = 1.89, 95% CI: 1.04-3.49). Each 10 μg/m increase in PM during the 2nd trimester was associated with 17.70% (2.21-33.20), 15.99% (2.96-29.01), 18.82% (4.11-33.52), and 17.10% (3.28-30.92) increase in 1-h, 2-h, Δ1h-fasting (1-h minus fasting), and Δ2h-fasting (2-h minus fasting) blood glucose levels, respectively. PM exposure at 24th-27th weeks after LMP was associated with increased GDM risk. We identified sensitive exposure windows of 21st-24th weeks for higher 1-h and 2-h blood glucose levels and of 20th-22nd weeks for increased Δ1h-fasting and Δ2h-fasting.

Conclusions: Ambient PM exposure during the second trimester was associated with higher odds of GDM and higher blood glucose levels. Avoiding exposure to high air pollution levels during the sensitive windows might prevent women from developing GDM.
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http://dx.doi.org/10.1016/j.envres.2022.114008DOI Listing
August 2022

E2F7 enhances hepatocellular carcinoma growth by preserving the SP1/SOX4/Anillin axis via repressing miRNA-383-5p transcription.

Mol Carcinog 2022 Aug 4. Epub 2022 Aug 4.

Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, P.R. China.

E2F family participates in most human malignancies by activating the transcription of the cell cycle-related genes. Whereas, as a specifical atypical member of this family, E2F7 was described as a repressor against its downstream genes and exerted oscillatory and controversial functions in cancers. Our previous study identified a molecular interaction promoting hepatocellular carcinoma (HCC) growth induced by SOX4 and Anillin. Meanwhile, we preliminarily identified SP1 as the upstream activator of SOX4. Intriguingly, we observed that the repressive E2F7 presents a remarkable high expression in HCC, and is positively correlated and involved in the same pathway with the potentially SP1/SOX4/Anillin axis. However, their exact interaction or mechanism controlling tumor progress between these genes has not been illustrated. Thus, we focused on this point in this study and attempted to improve the potential regulating axis in HCC cell proliferation and tumor growth for promoting tumor prevention and control. The expression profile of E2F7 in HCC tissues and tumor cells was detected along with the related candidate genes, through real-time quantitative polymerase chain reaction assay, the Western blot analysis, and the immunohistochemistry assay, combined with bioinformatics analysis of the HCC information from the the Cancer Genome Altas and Gene Expression Omnibus data sets. The correlation between E2F7 and HCC patients' clinicopathologic features was explored. Gain-of and loss-of-function assays were conducted both in vitro and in vivo along with the rescue experiment, for revealing the relative genes' functions in HCC progress. The ChIP and the dual-luciferase reporter assays were performed to verify the transcriptional regulating profile between E2F7 and SP1/SOX4/Anillin axis. E2F7 was upregulated in HCC and significantly correlated with SP1/SOX4/Anillin axis. High E2F7 expression is associated with dismal clinicopathologic features and poor survival of the patients. E2F7 depletion potently impaired SP1/SOX4/Anillin expression and significantly inhibited HCC growth. Furthermore, intensive exploration demonstrated that E2F7 preserves high SP1 levels by abrogating miR-383-5p in a transcriptional way. Atypical E2F7 is an important repressive transcription factor commonly upregulated in the HCC environment. E2F7 facilitates HCC growth by repressing miR-383-5p transcription and sequentially promoting SP1/SOX4/Anillin axis. Our findings provide us with probable targets for HCC prevention and therapeutic treatment.
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http://dx.doi.org/10.1002/mc.23454DOI Listing
August 2022

Real-time monitoring of the hydrolysis of vegetable oils loaded in silica colloidal crystal films with lipase by optical interferometry.

Anal Methods 2022 Aug 4. Epub 2022 Aug 4.

State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.

Different vegetable oils have different nutritional components, especially in terms of the composition of their fatty acids, which can only be reflected after entering the human body. Therefore, when judging their health value and identifying high-quality vegetable oils, in addition to the analysis of their ingredients, tracking their hydrolysis process in the human body is a very important aspect. However, most identification methods or simulated digestion studies fail to achieve this. In this paper, we applied ordered porous layer interferometry (OPLI) for the real-time monitoring of optical thickness changes (ΔOT) to track the hydrolysis process of four kinds of vegetable oil. Further, this study can obtain precise data (the initial rate and degree of hydrolysis) to provide more information on the hydrolysis ability of different vegetable oils and give references for their nutritional and functional evaluation. In addition, it provides more possibilities for the adulteration identification and bioavailability analysis of vegetable oils.
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http://dx.doi.org/10.1039/d2ay00811dDOI Listing
August 2022

Silk Fibroin-Induced Gadolinium-Functionalized Gold Nanoparticles for MR/CT Dual-Modal Imaging-Guided Photothermal Therapy.

J Funct Biomater 2022 Jun 22;13(3). Epub 2022 Jun 22.

Institute for Regenerative Medicine, Jian Hospital & Shanghai East Hospital, School of Life Sciences and Technology & School of Medicine, Tongji University, Shanghai 200092, China.

The development of multifunction nanoplatforms integrating accurate diagnosis and efficient therapy is of great significance for the precise treatment of tumors. Gold nanoparticles (AuNPs) possessing hallmark features of computed tomography (CT) imaging and photothermal conversion capability hold great potential in tumor theranostics. In this study, taking the advantages of outstanding biocompatibility, interesting anti-inflammatory and immunomodulatory properties, and abundant amino acid residues of silk fibroin (SF), a multifunctional Gd-hybridized AuNP nanoplatform was constructed using SF as a stabilizer and reductant via a facile one-pot biomimetic method, denoted as Gd:[email protected] The obtained Gd:[email protected] possessed fascinating biocompatibility and excellent photothermal conversion efficiency. Functionalized with Gd, Gd:[email protected] exhibited super tumor-contrasted imaging performance in magnetic resonance (MR) and CT imaging modalities. Moreover, Gd:[email protected], with strong NIR absorbance, demonstrated that it could effectively kill tumor cells in vitro, and was also proved to successfully ablate tumor tissues through MR/CT imaging-guided photothermal therapy (PTT) without systemic toxicity in Pan02 xenograft C57BL/6 mouse models. We successfully synthesized Gd:[email protected] for MR/CT dual-mode imaging-guided PTT via a facile one-pot biomimetic method, and this biomimetic strategy can also be used for the construction of other multifunction nanoplatforms, which is promising for precise tumor theranostics.
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http://dx.doi.org/10.3390/jfb13030087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326592PMC
June 2022

Co-fermented cow milk protein by Lactobacillus helveticus KLDS 1.8701 and Lactobacillus plantarum KLDS 1.0386 attenuates its allergic immune response in Balb/c mice.

J Dairy Sci 2022 Jul 22. Epub 2022 Jul 22.

Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China; Food College, Northeast Agricultural University, Harbin 150030, China; Heilongjiang Key Laboratory of Genetic and Metabolic Engineering of Lactic Acid Bacteria, Harbin 150030, China.

Milk protein is one of the major food allergens. As an effective processing method, fermentation may reduce the potential allergenicity of allergens. This study aimed to evaluate the therapeutic potential of co-fermented milk protein using Lactobacillus helveticus KLDS 1.8701 and Lactobacillus plantarum KLDS 1.0386 in cow milk protein allergy (CMPA) management. This study determined the secondary and tertiary structures of the fermented versus unfermented proteins by Fourier-transform infrared spectroscopy and surface hydrophobicity to evaluate its conformational changes. Our results showed that different fermentation methods have significantly altered the conformational structures of the cow milk protein, especially the tertiary structure. Further, the potential allergenicity of the fermented cow milk protein was assessed in Balb/c mice, and mice treated with the unfermented milk and phosphate-buffered saline were used as a control. We observed a significant reduction in allergenicity via the results of the spleen index, serum total IgE, specific IgE, histamine, and mouse mast cell protease 1 in the mice treated with the co-fermented milk protein. In addition, we analyzed the cytokines and transcription factors expression levels of spleen and jejunum and confirmed that co-fermentation could effectively reduce the sensitization of cow milk protein by regulating the imbalance of T helper (Th1/Th2 and Treg/Th17). This study suggested that changes of conformational structure could reduce the potential sensitization of cow milk protein; thus, fermentation may be a promising strategy for developing a method of hypoallergenic dairy products.
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http://dx.doi.org/10.3168/jds.2022-21844DOI Listing
July 2022

Omicron Booster in Ancestral Strain Vaccinated Mice Augments Protective Immunities Against Both Delta and Omicron Variants.

Front Immunol 2022 6;13:897879. Epub 2022 Jul 6.

Department of Infectious Diseases, Shanghai Key Laboratory of Infectious Diseases and Biosafety Emergency Response, National Medical Center for Infectious Diseases, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China.

A booster vaccination is called for constraining the evolving epidemic of SARS-CoV-2. However, the necessity of a new COVID-19 vaccine is currently unclear. To compare the effect of an Omicron-matched S DNA vaccine and an ancestral S DNA vaccine in boosting cross-reactive immunities, we firstly immunized mice with two-dose of a DNA vaccine encoding the spike protein of the ancestral Wuhan strain. Then the mice were boosted with DNA vaccines encoding spike proteins of either the Wuhan strain or the Omicron variant. Specific antibody and T cell responses were measured at 4 weeks post boost. Our data showed that the Omicron-matched vaccine efficiently boosted RBD binding antibody and neutralizing antibody responses against both the Delta and the Omicron variants. Of note, antibody responses against the Omicron variant elicited by the Omicron-matched vaccine were much stronger than those induced by the ancestral S DNA vaccine. Meanwhile, CD8 T cell responses against both the ancestral Wuhan strain and the Omicron strain also tended to be higher in mice boosted by the Omicron-matched vaccine than those in mice boosted with the ancestral S DNA vaccine, albeit no significant difference was observed. Our findings suggest that an Omicron-matched vaccine is preferred for boosting cross-protective immunities.
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http://dx.doi.org/10.3389/fimmu.2022.897879DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9298979PMC
July 2022

MiR-208b/miR-21 Promotes the Progression of Cardiac Fibrosis Through the Activation of the TGF-β1/Smad-3 Signaling Pathway: An and Study.

Front Cardiovasc Med 2022 5;9:924629. Epub 2022 Jul 5.

Department of Cardiology, Ninth Hospital of Xi'an, Xi'an, China.

Background: Regulatory molecule microRNAs (miRNAs) have been implicated in myocardial fibrosis. However, the specific mechanism by which they lead to myocardial fibrosis remains unclear. This study aimed to explore the roles of miR-208b, miR-21 and transforming growth factor-β1 (TGF-β1)/Smad-3 signaling pathway components in cardiac fibrosis development.

Materials And Methods: Thirty-six consecutive acute myocardial infarction (AMI) patients were included in this study. Plasma was collected on admission and at 24 h, 48 h and 6 d. The levels of plasma miR-208b, miR-21, TGF-β1, and Smad-3 were measured using reverse transcription-quantitative polymerase chain reaction (RT-qPCR), and cardiac calcium protein T (cTnT) and creatine kinase isoenzyme (CK-MB) were detected by electrochemiluminescence analysis. H9C2 cells were exposed to hypoxia and divided into 4 groups (hypoxia treatment for 6 h, 24 h, 48 h, and 72 h). These stimulated cells were then transfected with miRNA inhibitors and mimics for gene overexpression and inhibition. RT-qPCR was used to detect the expression of miR-208b, miR-21, TGF-β1, and Smad-3, and western blot analysis was used to detect TGF-β1 and Smad-3 protein expression.

Results: The plasma analysis showed cTnT and CK-MB expression peaked at 24 h after symptom onset; miR-208b, miR-21, TGF-β1, and Smad-3 levels showed no peak and increased gradually with time. Cell experiments revealed that miR-208b and TGF-β1 were upregulated along with increased hypoxia exposure; miR-21 expression peaked at 24 h and 72 h, with the highest peak at 72 h, and Smad-3 expression peaked at 6 h and 72 h, with the highest peak at 72 h. miR-208b and miR-21 expressions were positively correlated with TGF-β/Smad-3 expression. TGF-β1/Smad-3 mRNA and protein levels were elevated in the miR-208b and miR-21 overexpression groups and reduced in the miR-208b and miR-21 inhibition groups.

Conclusion: MiR-208b and miR-21 promote cardiac fibrosis progression through TGF-β1/Smad-3 signaling pathway activation.
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http://dx.doi.org/10.3389/fcvm.2022.924629DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294285PMC
July 2022

Correlation between Blood Oxygen Level-Dependent Magnetic Resonance Imaging Images and Prognosis of Patients with Multicenter Diabetic Nephropathy on account of Artificial Intelligence Segmentation Algorithm.

Comput Math Methods Med 2022 11;2022:5700249. Epub 2022 Jul 11.

Department of Nephrology, Wenzhou Central Hospital, Wenzhou, 325000 Zhejiang, China.

This study was aimed to analyze the correlation between blood oxygen level-dependent magnetic resonance imaging (BOLD-MRI) images and prognosis of patients with diabetic nephropathy (DN) based on artificial intelligence (AI) segmentation algorithm, so as to provide references for diagnosis and treatment as well as prognosis analysis of patients DN. In this study, a kernel function-based fuzzy C-means algorithm (KFCM) model was proposed, and the FCM algorithm based on neighborhood pixel information (BCFCM) and the FCM algorithm based on efficiency improvement (EnFCM) were introduced for comparison to analyze the image segmentation effects of three algorithms. The results showed that the partition coefficient (V) and partition entropy (V) of the KFCM algorithm were 0.801 and 0.602, respectively, which were better than those of the traditional FCM, BCFCM, and EnFCM algorithm. At the same time, the effects of correlation between renal cortex R2∗ (RC-R2∗), renal medulla R2∗ (RM-R2∗), renal cortex D (RC-D), renal medulla D (RM-D) and renal function on the prognosis were compared. The results showed that the correlation coefficients between RC-R2∗, RM-R2∗, RC-D, RM-D and renal function were 0.57, 0.62, 0.49, and 0.38, respectively; among them, RC-R2∗ and RM-R2∗ were negatively correlated to the estimated glomerular filtration rate (eGFR), and the difference between the groups was statistically significant ( <0.05). Among the factors affecting the prognosis of DN patients, the GFR, hemoglobin (Hb), RC-R2∗, RM-R2∗, and RC-D were all related to the prognosis of DN, and the difference between groups was statistically obvious ( <0.05). It suggested that the KFCM algorithm proposed in this study showed the relatively best segmentation effect on BOLD-MRI images for DN patients; an increase in R2∗ indicated a poor prognosis, and an increase in the RC-D value indicated a better prognosis.
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http://dx.doi.org/10.1155/2022/5700249DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9293502PMC
July 2022

Antineoplastic Enzyme as Drug Carrier with Activatable Catalytic Activity for Efficient Combined Therapy.

Angew Chem Int Ed Engl 2022 Jul 18:e202208583. Epub 2022 Jul 18.

Marshall Laboratory of Biomedical Engineering, International Cancer Center, Laboratory of Evolutionary Theranostics (LET), School of Biomedical Engineering, Shenzhen University Health Science Center, Shenzhen, 518060, China.

The delivery of glucose oxidase (GOx) requires extra carriers, which suffers from early leakage and exposure of GOx. These issues will be of less concern if GOx itself acts as a drug carrier. However, the catalytic activity of GOx in the blood still needs to be inhibited. Herein, we found that GOx could self-assemble with hydrophobic molecules into uniform and stable nanoparticles (NPs), including sorafenib, 4'-(amino-methyl phenyl)-2,2' : 6',2''-terpyridine modified cyanin and paclitaxel. The catalytic activity of GOx in NPs was significantly inhibited due to the binding of small molecules with its hydrophobic pockets. After dissociation in the tumor acidic microenvironment, the enzyme activity of GOx could be largely recovered. This acidity-triggered "OFF-to-ON" process ensured safe intravenous administration of GOx-based NPs. In vivo experiments showed that the combined starvation therapy and ferroptosis/photothermal therapy/chemotherapy effectively inhibited 4T1 breast tumor growth.
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http://dx.doi.org/10.1002/anie.202208583DOI Listing
July 2022

Clinical Oral Condition Analysis and the Influence of Highly Active Antiretroviral Therapy on Human Salivary Microbial Community Diversity in HIV-Infected/AIDS Patients.

Front Cell Infect Microbiol 2022 29;12:937039. Epub 2022 Jun 29.

Department of Stomatology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.

The purpose of this study was to assess the clinical oral status and investigate the effect of highly active antiretroviral therapy (HAART) on oral flora diversity in human immunodeficiency virus (HIV)-infected/acquired immune deficiency syndrome (AIDS) patients. We first recorded and analyzed the demographic indicators of 108 HIV-infected patients and assessed their periodontal health, dental health and oral lesion status by oral examination. Besides, we compared the changes in salivary microbial communities of healthy controls, before and after treatment of HAART-processed AIDS patients by Roche 454 sequencing and RT-qPCR. In HIV-infected/AIDS patients, age, sex, marital status, income level, smoking and oral health behaviors had an effect on periodontal clinical indicators; age and marital status were correlated with dental clinical indicators; most of them were accompanied by oral manifestations, mainly including candidiasis albicans, salivary gland disease, AIDS-associated periodontitis, and oral ulcers. Besides, a total of 487 species were detected in the saliva of AIDS patients. The microbial communities of HAART-unprocessed AIDS patients significantly differed from those processed patients, with 112 unique microbial species. More importantly, a large number of conditioned pathogens were also detected in the saliva samples of AIDS patients, which may be associated with opportunistic infections. Therefore, HAART might have a crucial role in salivary microecological balance in AIDS patients. And these patients should pay attention to the maintenance of oral health, and the early initiation of HAART may be important for the development of oral lesions.
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http://dx.doi.org/10.3389/fcimb.2022.937039DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277119PMC
July 2022

The Association Between Morningness-Eveningness Preference, Depression, Anxiety and Insomnia Among Chinese Textile Workers With or Without Shift Work.

Front Psychiatry 2022 30;13:915476. Epub 2022 Jun 30.

School of Psychology, Centre for Studies of Psychological Applications, Guangdong Key Laboratory of Mental Health and Cognitive Science, Ministry of Education Key Laboratory of Brain Cognition and Educational Science, South China Normal University, Guangzhou, China.

Objective: Circadian preference and mental health disorders are closely related to insomnia. This study aimed to evaluate insomnia symptoms in textile factory workers with different work schedules, and to investigate the association between insomnia, morningness-eveningness preference, anxiety, and depression.

Methods: A total of 3,883 textile workers were assessed using the 3-items of Pittsburg Sleep Quality Index, Composite Scale of Morningness, Beck Anxiety Inventory, Center for Epidemiologic Studies-Depression Scale, and socio-demographic questionnaires.

Results: The prevalence rate of insomnia in textile workers was 16.7% ( = 646), with 49.8% ( = 322) were shift workers. Among shift workers ( = 1,833), 9.5% had difficulty initiating sleep, and almost 9.0% suffered from early morning awakening, a rate significantly higher than among daytime workers. Logistics regressions revealed that work schedule was insignificantly associated with insomnia. Depression (OR = 1.034, 95% CI = 1.022-1.046) and anxiety (OR = 1.031, 95% CI = 1.018-1.043) positively predicted insomnia, whereas morningness preference (OR = 0.977, 95% CI = 0.960-0.995) decreased the likelihood of insomnia. Furthermore, mediation analysis showed that both anxiety and depression independently mediated the association between circadian preferences and insomnia in textile workers with or without shift work.

Conclusion: This study highlighted the insomnia, depression, and anxiety of textile factory workers in a Chinese textile factory. To improve insomnia symptoms, interventions to promote morningness circadian preference and reduce depressive and anxious symptoms among workers are encouraged.
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http://dx.doi.org/10.3389/fpsyt.2022.915476DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279731PMC
June 2022

Imaging of Tumor-Associated Vascular Prostate-Specific Membrane Antigen in Woodchuck Model of Hepatocellular Carcinoma.

Gastro Hep Adv 2022 28;1(4):631-639. Epub 2022 Apr 28.

Radiology, Case Western Reserve University, Cleveland, Ohio.

Background And Aims: Radiolabeled short peptide ligands targeting prostate-specific membrane antigen (PSMA) were developed initially for imaging and treatment of prostate cancers. While many nonprostate solid tumors including hepatocellular carcinoma (HCC) express little PSMA, their neovasculature expresses a high level of PSMA, which is avid for Gallium-68-labeled PSMA-targeting radio-ligand (Ga-PSMA-11) for positron emission tomography (PET). However, the lack of a spontaneous animal model of tumor-associated vascular PSMA overexpression has hindered the development and assessment of PSMA-targeting radioligands for imaging and therapy of the nonprostatic cancers. We identified detectable indigenous PSMA expression on tumor neovascular endothelia in a naturally occurring woodchuck model of HCC.

Methods: Molecular docking was performed with 3 bait PSMA ligands and compared between human and woodchuck PSMA. Initially, PET images were acquired dynamically after intravenously injecting 37 MBq (1.0 mCi) of Ga-PSMA-11 into woodchuck models of HCC. Subsequently, 10-minute static PET scans were conducted for other animals 1-hour after injection due to HCC and liver background uptake stabilization at 30-45 minutes after injection. Liver tissue samples were harvested after imaging, fresh-frozen for quantitative reverse transcription polymerase chain reaction and western blot for validation, or fixed for histology for correlation.

Results: Our preclinical studies confirmed the initial clinical findings of Ga-PSMA-11 uptake in HCC. The agents (ligands and antibodies) developed against human PSMA were found to be reactive against the woodchuck PSMA.

Conclusion: This animal model offers a unique opportunity for investigating the biogenesis of tumor-associated vascular PSMA, its functional role(s), and potentials for future treatment strategies targeting tumor vascular PSMA using already developed PSMA-targeting agents.
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http://dx.doi.org/10.1016/j.gastha.2022.04.014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9280909PMC
April 2022

Clustering analysis and prognostic signature of lung adenocarcinoma based on the tumor microenvironment.

Sci Rep 2022 Jul 14;12(1):12059. Epub 2022 Jul 14.

Department of Respiration, West China Hospital of Sichuan University, 37# Guo Xue Xiang, Chengdu, 610041, Sichuan, China.

Because of immunotherapy failure in lung adenocarcinoma, we have tried to find new potential biomarkers for differentiating different tumor subtypes and predicting prognosis. We identified two subtypes based on tumor microenvironment-related genes in this study. We used seven methods to analyze the immune cell infiltration between subgroups. Further analysis of tumor mutation load and immune checkpoint expression among different subgroups was performed. The least absolute shrinkage and selection operator Cox regression was applied for further selection. The selected genes were used to construct a prognostic 14-gene signature for LUAD. Next, a survival analysis and time-dependent receiver operating characteristics were performed to verify and evaluate the model. Gene set enrichment analyses and immune analysis in risk groups was also performed. According to the expression of genes related to the tumor microenvironment, lung adenocarcinoma can be divided into cold tumors and hot tumors. The signature we built was able to predict prognosis more accurately than previously known models. The signature-based tumor microenvironment provides further insight into the prediction of lung adenocarcinoma prognosis and may guide individualized treatment.
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http://dx.doi.org/10.1038/s41598-022-15971-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283441PMC
July 2022

Enzymatic Crosslinked Silk Fibroin Hydrogel for Biodegradable Electronic Skin and Pulse Waveform Measurements.

Biomacromolecules 2022 08 13;23(8):3429-3438. Epub 2022 Jul 13.

College of Chemistry and Molecular Sciences, Hubei Engineering Center of Natural Polymer-Based Medical Materials, Wuhan University, Wuhan 430072, China.

The development of a portable, controllable, and environmentally friendly electronic skin (e-skin) is highly desirable; however, it presents a major challenge. Herein, a biocompatible, biodegradable, and easily usable hydrogel was designed and fabricated as e-skin to enable the transmission of information regarding the spatial pressure distribution. Silk fibroin (SF) was used as the hydrogel skeleton, which endowed the hydrogel with intelligent mechanical sensitivity. During its conditioning in weakly acidic media, the density of the enzymatic crosslink increased and a dense network was formed due to the formation of covalent/hydrogen bonds. Additionally, a conductive SF/polyvinyl alcohol (PVA) hybrid film was molded as a flexible electrode after graphite deposition. The above SF sensing unit based on SF hydrogels and SF/PVA hybrid films showed high strain sensitivity (4.78), fast responsiveness (<0.1 s), good cycling stability (≥10,000), excellent biocompatibility, and biodegradability. Importantly, a coplanar 8 × 8 pixel SF-based e-skin array was successfully fabricated and applied for 3D signal transmission of the object. The SF-based e-skin was capable of precisely tracking the changes in the pulse pressure, the movement of the finger joint, and the vibrations of the vocal cord. Therefore, the current findings provide a solid foundation for future studies exploring the next generation of electronic devices.
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http://dx.doi.org/10.1021/acs.biomac.2c00553DOI Listing
August 2022

Raising of Anillin expression in para-cancerous hepatocytes is associated with hepatic depolyploidization and short-term recurrence of hepatocellular carcinoma after radical operation.

J Cancer 2022 29;13(9):2729-2739. Epub 2022 May 29.

Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197, Rui Jin Er Road, Shanghai 200025, People's Republic of China.

Short-term recurrence of hepatocellular carcinoma (HCC) after radical operation results in poor prognosis and short overall survival period. Assessment of the post-operational recurrence risk could provide an advantage for preventing lethal progress by assisting to set up necessarily individual adjuvant treatment. Here, on basis of our previous research on the ploidy status of hepatocytes, we detected the expression of Anillin, a pivotal regulating gene of depolyploidization in para-cancerous hepatocytes, and discussed the relation between its alternative expression and short-term recurrence of HCC patients after radical operation. One hundred and twenty-one specimens of the para-cancerous tissues from a cohort of HCC patients were collected. The RT-qPCR assay and the Immunohistochemistry assay were conducted for a thorough profile of Anillin expression, along with the analysis of patients' information from the GEO database. The clinicopathological para-maters of the patients were detected to determine the relationship between Anillin in the para--cancerous and the tumor relapse. Specimens treated with HE staining were examined for the precise count of the binuclear polyploid hepatocytes, and the karyoplasmic ratio was calculated. Anillin was verified raised in the para-cancerous tissues of the patients who relapsed. The raising of Anillin in para-cancerous tissue is related to poor clinicopathologic features of HCC patients and short-term recurrence. Binuclear polyploid hepatocytes were reduced along with a higher expression of Anillin, and the proportion of high karyoplasmic ratio hepatocytes was significantly decreased. The raising of Anillin is associated with the depolyploidization of hepatocytes in the representation of losing binuclear hepatocytes and reducing proportion reduction of high karyoplasmic ratio hepatocytes. Measuring Anillin in para-cancerous tissue, along with the pathomorphological examination may provide us a strategy for screening high risk of HCC recurrence, and help to design individual adjuvant treatment post-operation.
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http://dx.doi.org/10.7150/jca.72890DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9254876PMC
May 2022

Is a Positive Regulator of Tolerance to Drought and Salt Stresses in Rice.

Plants (Basel) 2022 Jun 23;11(13). Epub 2022 Jun 23.

Crop Ecophysiology and Cultivation Key Laboratory of Sichuan Province, Rice Research Institute of Sichuan Agricultural University, Chengdu 611130, China.

Rice ( L.) is one of the main food crops for human survival, and its yield is often restricted by abiotic stresses. Drought and soil salinity are among the most damaging abiotic stresses affecting today's agriculture. Given the importance of abscisic acid (ABA) in plant growth and abiotic stress responses, it is very important to identify new genes involved in ABA signal transduction. We screened a drought-inducing gene containing about 158 amino acid residues from the transcriptome library of rice exposed to drought treatment, and we found ABA-related cis-acting elements and multiple drought-stress-related cis-acting elements in its promoter sequence. The results of real-time PCR showed that was strongly induced by drought and salt stresses. The physiological and biochemical phenotype analysis of transgenic plants confirmed that overexpression of enhanced the tolerance to drought and salt stresses in rice. The expression of -GFP fusion protein indicated that was located in both the cell membrane system and nucleus. Compared with the wild type, the overexpressed showed enhanced sensitivity to ABA. Physiological analyses showed that the overexpression of may regulate the water loss efficiency and ABA-responsive gene expression of rice plants under drought and salt stresses, and it reduces membrane damage and the accumulation of reactive oxygen species. These results indicate that is a positive regulator of drought and salinity tolerance in rice, governing the tolerance of rice to abiotic stresses through an ABA-dependent pathway. Therefore, this study provides a new insight into the physiological and molecular mechanisms of -mediated ABA signal transduction participating in drought and salt stresses.
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http://dx.doi.org/10.3390/plants11131653DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9269302PMC
June 2022

Tuning Microstructure and Mechanical Performance of a Co-Rich Transformation-Induced Plasticity High Entropy Alloy.

Materials (Basel) 2022 Jun 30;15(13). Epub 2022 Jun 30.

Institute for Materials Research, Tohoku University, 2-1-1 Katahira, Sendai 980-8577, Miyagi, Japan.

Multi-principal element alloys and high-entropy alloys (HEAs) are emerging metallic materials with unprecedented structures and properties for various applications. In this study, we tuned the microstructure and mechanical performance of a recently designed high-performance Co-rich TRIP-HEA via thermomechanical processing (TMP). The microstructures of the HEA after various TMP routines were characterized, and their correlation with room-temperature tensile performance was clarified. The results showed that grain refinement is an effective strategy for enhancing strength while retaining satisfactory ductility. The formation of incoherent precipitates slightly improves the strength but inevitably sacrifices the ductility, which needs to be considered for optimizing the TMPs. The room temperature tensile yield strength and ultimate tensile strength were increased from 254.6 to 641.3 MPa and from 702.5 to 968.4 MPa, respectively, but the tensile elongation retains a satisfactory value of 68.8%. We herein provide important insights into the regulation of the microstructure and mechanical properties of TRIP-HEAs.
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http://dx.doi.org/10.3390/ma15134611DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9267696PMC
June 2022

A novel signature derived from metabolism-related genes GPT and SMS to predict prognosis of laryngeal squamous cell carcinoma.

Cancer Cell Int 2022 Jul 8;22(1):226. Epub 2022 Jul 8.

Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, 200031, China.

Background: A growing body of evidence has suggested the involvement of metabolism in the occurrence and development of tumors. But the link between metabolism and laryngeal squamous cell carcinoma (LSCC) has rarely been reported. This study seeks to understand and explain the role of metabolic biomarkers in predicting the prognosis of LSCC.

Methods: We identified the differentially expressed metabolism-related genes (MRGs) through RNA-seq data of The Cancer Genome Atlas (TCGA) and Gene set enrichment analysis (GSEA). After the screening of protein-protein interaction (PPI), hub MRGs were analyzed by least absolute shrinkage and selection operator (LASSO) and Cox regression analyses to construct a prognostic signature. Kaplan-Meier survival analysis and the receiver operating characteristic (ROC) was applied to verify the effectiveness of the prognostic signature in four cohorts (TCGA cohort, GSE27020 cohort, TCGA-sub1 cohort and TCGA-sub2 cohort). The expressions of the hub MRGs in LSCC cell lines and clinical samples were verified by quantitative reverse transcriptase PCR (qRT-PCR). The immunofluorescence staining of the tissue microarray (TMA) was carried out to further verify the reliability and validity of the prognostic signature. Cox regression analysis was then used to screen for independent prognostic factors of LSCC and a nomogram was constructed based on the results.

Results: Among the 180 differentially expressed MRGs, 14 prognostic MRGs were identified. A prognostic signature based on two MRGs (GPT and SMS) was then constructed and verified via internal and external validation cohorts. Compared to the adjacent normal tissues, SMS expression was higher while GPT expression was lower in LSCC tissues, indicating poorer outcomes. The prognostic signature was proven as an independent risk factor for LSCC in both internal and external validation cohorts. A nomogram based on these results was developed for clinical application.

Conclusions: Differentially expressed MRGs were found and proven to be related to the prognosis of LSCC. We constructed a novel prognostic signature based on MRGs in LSCC for the first time and verified it via different cohorts from both databases and clinical samples. A nomogram based on this prognostic signature was developed.
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http://dx.doi.org/10.1186/s12935-022-02647-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9270735PMC
July 2022

Lactobacillus plantarum-derived extracellular vesicles protect against ischemic brain injury via the microRNA-101a-3p/c-Fos/TGF-β axis.

Pharmacol Res 2022 Aug 30;182:106332. Epub 2022 Jun 30.

Department of Neurology, Affiliated Hospital of Guizhou Medical University, Guizhou Medical University, Guiyang, China; Department of Translational Medicine Research Center,Guizhou Medical University, Guiyang, China. Electronic address:

Currently, the reported source of extracellular vesicles (EVs) for the treatment of ischemic stroke(IS)is limited to mammals. Moreover, these EVs are restricted to clinical translation by the high cost of cell culture. In this respect, Lactobacillus plantarum culture is advantaged by low cost and high yield. However, it is poorly understood whether Lactobacillus plantarum-derived EVs (LEVs) are applicable for the treatment of IS. Here, our results demonstrated that LEVs reduced apoptosis in ischemic neuron both in vivo and in vitro. As revealed by high-throughput sequencing, miR-101a-3p expression was significantly elevated by LEV treatment in OGD/R-induced neurons, as confirmed in the tMCAO mice treated with LEVs. Mechanistically, c-Fos was directly targeted by miR-101a-3p. In addition, c-Fos determined ischemia-induced neuron apoptosis in vivo and in vitro through the TGF-β1 pathway, miR-101a-3p inhibition aggravated ischemia-induced neuron apoptosis in vitro and in vivo, and miR-101a-3p overexpression produced the opposite results. Hsa-miR-101-3p was downregulated in the plasma of patients with IS but upregulated in the patients with neurological recovery after rt-PA intravenous thrombolysis. In conclusion, Our results demonstrated for the first time that LEVs might inhibit neuron apoptosis via the miR-101a-3p/c-Fos/TGF-β axis, and has-miR-101-3p is a potential marker of neurological recovery in IS patients.
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http://dx.doi.org/10.1016/j.phrs.2022.106332DOI Listing
August 2022

Overexpressed LINC01510 Attenuates Epithelial-Mesenchymal Transition in High Glucose-Induced Renal Tubular Epithelial Cells via Inactivating MMP7.

Ann Clin Lab Sci 2022 May;52(3):367-373

Department of Nephrology and Wenzhou Central Hospital, Wenzhou, China.

Objective: Matrix metallopeptidase 7 (MMP7) can promote renal fibrosis in diabetic kidney disease (DKD). A study found that LINC01510 overexpression inhibits MMP7 to play a role in renal cancer, but the relationship between the two in DKD had not been revealed, and the function of LINC01510 also needed to be explored, which was also the focus of this study.

Methods: The morphological changes of HK-2 cells induced by high glucose were observed by an inverted microscope, and fluorescence hybridization was used to determine the subcellular localization of LINC01510. Quantitative realtime polymerase chain reaction was used to detect the levels of LINC01510 and MMP7. The effect of LINC01510 and MMP7 overexpression on high glucose-induced HK-2 cell migration and epithelial-mesenchymal transition (EMT)-related protein changes was confirmed by wound healing experiments and western blot.

Results: High glucose induced HK-2 cells to gradually lose their epithelial phenotype, and decreased LINC01510 in a time-dependent manner. LINC01510 was located in the nucleus of HK-2 cells. LINC01510 overexpression increased the level of LINC01510, inhibited cell migration, and reduced the expression of MMP-7, Vimentin, α-SMA, and Fibronectin protein, and promoted the expression of E-cadherin protein in high glucose-induced cells. The effect of MMP7 overexpression on migration and EMT-related proteins was opposite to the effect of LINC01510 overexpression, and partially reversed the effect of LINC01510 overexpression in high glucose-induced cells.

Conclusion: Our research shows that LINC01510 overexpression inactivates MMP7 to attenuate high glucose-induced EMT of renal tubular epithelial cells.
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May 2022

Effects of shrub-grass cover on the hillslope overland flow and soil erosion under simulated rainfall.

Environ Res 2022 Jun 28;214(Pt 1):113774. Epub 2022 Jun 28.

Jixian National Forest Ecosystem Observation and Research Station, CNERN, School of Soil and Water Conservation, Beijing Forestry University, Beijing, 100083, PR China; Key Laboratory of Soil and Water Conservation and Desertification Combating, State Forestry and Grassland Administration, PR China. Electronic address:

Vegetation plays a vital role in regulating hydrological cycle and controlling soil erosion at multiple spatial and temporal scales. Establishing shrub-grass community is one of the widely adopted practices to increase rainfall infiltration and reduce soil erosion in water-limited and highland regions. To understand the effects of such vegetation communities on soil erosion and overland flow under different rainfall regimes at the hillslope scale, we conducted rainfall simulation experiments by setting up parallel plots at fixed slope of 15 including unvegetated (coverage 0%), shrub only (coverage 50%), grass only (coverage 50%), and shrub-grass covered (coverages 25%, 50%, 75%, and 100%) and constant rainfall intensities of 30, 60, and 90 mm h rainfalls lasting 60 min each after the initiation of overland flow. Two native species Lespedeza bicolor and Carex giraldiana, distributed in the soil sampling region were planted on the plots to achieve designed coverages. We found that the overland flow and sediment load from vegetated slopes were reduced by 9%-58% and 27%-98%, respectively, compared with unvegetated slopes while the infiltration rate increased by over 45%. Shrub-grass community reduced the overland flow and sediment yield more significantly than shrub only and grass only treatments with the same coverage of 50% under three rainfall intensities. In addition, the overland flow rate linearly decreased while the mean sediment yield exponentially reduced against the increase in shrub-grass community coverage. Hydrodynamically, shrub-grass communities not only increased the critical hydrodynamic forces for the initiating soil erosion but also increased the resistance coefficient leading to reduce overland flow velocity, stream power, and thus soil erosion from the vegetative slope even under extreme rainfalls. Our research highlights the importance of developing the shrub-grass communities to reduce the quantity and energy of overland flow and control soil erosion on the hillslopes in water-limited and highland regions.
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http://dx.doi.org/10.1016/j.envres.2022.113774DOI Listing
June 2022

Molecular mechanism of -adenosylmethionine sensing by SAMTOR in mTORC1 signaling.

Sci Adv 2022 07 1;8(26):eabn3868. Epub 2022 Jul 1.

State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China.

The mechanistic target of rapamycin-mLST8-raptor complex (mTORC1) functions as a central regulator of cell growth and metabolism in response to changes in nutrient signals such as amino acids. SAMTOR is an -adenosylmethionine (SAM) sensor, which regulates the mTORC1 activity through its interaction with the GTPase-activating protein activity toward Rags-1 (GATOR1)-KPTN, ITFG2, C12orf66 and SZT2-containing regulator (KICSTOR) complex. In this work, we report the crystal structures of SAMTOR in apo form and in complex with SAM. SAMTOR comprises an N-terminal helical domain and a C-terminal SAM-dependent methyltransferase (MTase) domain. The MTase domain contains the SAM-binding site and the potential GATOR1-KICSTOR-binding site. The helical domain functions as a molecular switch, which undergoes conformational change upon SAM binding and thereby modulates the interaction of SAMTOR with GATOR1-KICSTOR. The functional roles of the key residues and the helical domain are validated by functional assays. Our structural and functional data together reveal the molecular mechanism of the SAM sensing of SAMTOR and its functional role in mTORC1 signaling.
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http://dx.doi.org/10.1126/sciadv.abn3868DOI Listing
July 2022

Separating single- and multiple-scattering components in laser speckle contrast imaging of tissue blood flow.

Biomed Opt Express 2022 May 21;13(5):2881-2895. Epub 2022 Apr 21.

School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.

Random matrix theory provides new insights into multiple scattering in random media. In a recent study, we demonstrated the statistical separation of single- and multiple-scattering components based on a Wishart random matrix. The first- and second-order moments were estimated with a Wishart random matrix constructed using dynamically backscattered speckle images. In this study, this new strategy was applied to laser speckle contrast imaging (LSCI) of blood flow. The random matrix-based method was adopted and parameterized using electric field Monte Carlo simulations and blood flow phantom experiments. The new method was further applied to experiments, demonstrating the benefits of separating the single- and multiple-scattering components, and the method was compared with the traditional temporal laser speckle contrast analysis (LASCA) method. More specifically, the new method separates the stimulus-induced functional changes in blood flow and tissue perfusion in the superficial (<2 , is the transport mean free path) and deep layers (1  ∼ 7 ), extending LSCI to the evaluation of functional and pathological changes.
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http://dx.doi.org/10.1364/BOE.453412DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9203116PMC
May 2022

Deep oncopanel sequencing reveals within block position-dependent quality degradation in FFPE processed samples.

Genome Biol 2022 06 29;23(1):141. Epub 2022 Jun 29.

Q2 Solutions - EA Genomics, 5927 S Miami Blvd, Morrisville, NC, 27560, USA.

Background: Clinical laboratories routinely use formalin-fixed paraffin-embedded (FFPE) tissue or cell block cytology samples in oncology panel sequencing to identify mutations that can predict patient response to targeted therapy. To understand the technical error due to FFPE processing, a robustly characterized diploid cell line was used to create FFPE samples with four different pre-tissue processing formalin fixation times. A total of 96 FFPE sections were then distributed to different laboratories for targeted sequencing analysis by four oncopanels, and variants resulting from technical error were identified.

Results: Tissue sections that fail more frequently show low cellularity, lower than recommended library preparation DNA input, or target sequencing depth. Importantly, sections from block surfaces are more likely to show FFPE-specific errors, akin to "edge effects" seen in histology, while the inner samples display no quality degradation related to fixation time.

Conclusions: To assure reliable results, we recommend avoiding the block surface portion and restricting mutation detection to genomic regions of high confidence.
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http://dx.doi.org/10.1186/s13059-022-02709-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9241261PMC
June 2022

Configuration Sensitivity of Electrocatalytic Oxygen Reduction Reaction on Nitrogen-Doped Graphene.

J Phys Chem Lett 2022 Jul 29;13(26):6187-6193. Epub 2022 Jun 29.

School of Chemistry and Chemical Engineering, Guangdong Provincial Key Lab of Green Chemical Product Technology, South China University of Technology, Guangzhou 510641, China.

As one of the most promising nonprecious metal catalysts for the oxygen reduction reaction (ORR), the structure of the active site on nitrogen-doped carbon materials is still under debate. Here, we report that the sensitivity of the ORR on the local configuration of multiple nitrogen dopants may be overlooked. Combining global structure searching with density functional theory calculations, we established the structure-activity relationship for 19 and 298 possible configurations of graphitic nitrogen-doped graphene with N content of 2 and 3%, respectively. It was revealed that the stability cannot be a screener to determine the major contributor to the activity. 77.5% of current density is contributed by the active configuration with 4.59% population on the graphene containing 3% nitrogen. It unambiguously demonstrates the configuration sensitivity of N-doped graphene for ORR and opens a new window to identifying the optimal structure of N-doped carbons for various applications.
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http://dx.doi.org/10.1021/acs.jpclett.2c01645DOI Listing
July 2022

Boosted π-Li Cation Effect in the Stabilized Small Organic Molecule Electrode via Hydrogen Bonding with MXene.

ACS Appl Mater Interfaces 2022 Jul 25;14(26):29974-29985. Epub 2022 Jun 25.

School of Environmental and Chemical Engineering, Shanghai University, 99 Shangda Road, Shanghai, P. R. China, 200444.

The high solubility of the small organic molecule materials in organic electrolytes hinders their development in rechargeable batteries. Hence, this work designs an ultrarobust hydrogen-bonded organic-inorganic hybrid material: the small organic unit of the 1,3,6,8-tetrakis (p-benzoic acid) pyrene (TBAP) molecule connected with the hydroxylated TiCT MXene through hydrogen bonds between the terminal groups of -COOH and -OH. The robust and elastic hydrogen bonds can empower the TBAP, despite being a low-molecule organic chemical, with unusually low solubility in organic electrolytes and thermal stability. The alkali-treated TiCT MXene provides a hydroxyl-rich conductive network, and the small organic molecule of TBAP reduces the restacking of MXene layers. Therefore, the combination of these two materials complements each other well, and this organic-inorganic [email protected] electrode delivers large reversible capacities and long cyclic life. Notably, with the assistance of the in situ FT-IR characterization of the electrode within the fully lithiated (0.005 V) and the delithiated (3.0 V) states, it is revealed that a powerful π-Li cation effect mainly governs the lithium-storage mechanism with the highly activated benzene ring and each C6 aromatic ring, which can reversibly accept six Li-ions to form a 1:1 Li/C complex.
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http://dx.doi.org/10.1021/acsami.2c08366DOI Listing
July 2022
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