Publications by authors named "Yifan Liu"

240 Publications

Discovery of Potent EGFR Inhibitors With Derivatives.

Front Pharmacol 2021 26;12:647591. Epub 2021 May 26.

Department of Medicinal Chemistry, Hebei Medical University, Shijiazhuang, China.

According to the classical pharmacophore fusion strategy, a series of derivatives ( - ) were designed, synthesized, and biologically evaluated by the standard CCK-8 method and enzyme inhibition assay. Among the title compounds, , , , , , , , and exhibited promising anti-proliferative bioactivities, especially , which had excellent antitumor activity against the A549, HT-29, and MCF-7 cell lines (IC = 2.25, 1.72, and 2.81 μM, respectively) compared with gefitinib, erlotinib, and sorafenib. In addition, the enzyme activity inhibition assay indicated that the synthesized compounds had sub-micromolar inhibitory levels (IC, 11.66-867.1 nM), which was consistent with the results of the tumor cell line growth inhibition tests. By comparing the binding mechanisms of (17.32 nM), gefitinib (25.42 nM), and erlotinib (33.25 nM) to the EGFR, it was found that could extend into the effective region with a similar action conformation to that of gefitinib and interact with residues L85, D86, and R127, increasing the binding affinity of to the EGFR. Based on the molecular hybridization strategy, 14 compounds with EGFR inhibitory activity were designed and synthesized, and the action mechanism was explored through computational approaches, providing valuable clues for the research of antitumor agents based on EGFR inhibitors.
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http://dx.doi.org/10.3389/fphar.2021.647591DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187944PMC
May 2021

Strategies for Vaccine Prioritization and Mass Dispensing.

Vaccines (Basel) 2021 May 14;9(5). Epub 2021 May 14.

Galveston National Laboratory, University of Texas Medical Branch, Galveston, TX 77550, USA.

We propose a system that helps decision makers during a pandemic find, in real time, the mass vaccination strategies that best utilize limited medical resources to achieve fast containments and population protection. Our general-purpose framework integrates into a single computational platform a multi-purpose compartmental disease propagation model, a human behavior network, a resource logistics model, and a stochastic queueing model for vaccination operations. We apply the modeling framework to the current COVID-19 pandemic and derive an optimal trigger for switching from a prioritized vaccination strategy to a non-prioritized strategy so as to minimize the overall attack rate and mortality rate. When vaccine supply is limited, such a mixed vaccination strategy is broadly effective. Our analysis suggests that delays in vaccine supply and inefficiencies in vaccination delivery can substantially impede the containment effort. Employing an optimal mixed strategy can significantly reduce the attack and mortality rates. The more infectious the virus, the earlier it helps to open the vaccine to the public. As vaccine efficacy decreases, the attack and mortality rates rapidly increase by multiples; this highlights the importance of early vaccination to reduce spreading as quickly as possible to lower the chances for further mutations to evolve and to reduce the excessive healthcare burden. To maximize the protective effect of available vaccines, of equal importance are determining the optimal mixed strategy and implementing effective on-the-ground dispensing. The optimal mixed strategy is quite robust against variations in model parameters and can be implemented readily in practice. Studies with our holistic modeling framework strongly support the urgent need for early vaccination in combating the COVID-19 pandemic. Our framework permits rapid custom modeling in practice. Additionally, it is generalizable for different types of infectious disease outbreaks, whereby a user may determine for a given type the effects of different interventions including the optimal switch trigger.
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http://dx.doi.org/10.3390/vaccines9050506DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157047PMC
May 2021

Comparison of chondro-inductivity between collagen and hyaluronic acid hydrogel based on chemical/physical microenvironment.

Int J Biol Macromol 2021 May 29;182:1941-1952. Epub 2021 May 29.

National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 61004, Sichuan, China.

Achieving chondrogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) successfully is crucial for cartilage regeneration. To date, various hydrogels with different chemical microenvironment have been used to modulate chondrogenic differentiation of BMSCs, especially collagen and hyaluronic acid hydrogel. However, the chondro-inductive ability of collagen and hyaluronic acid hydrogel has not been evaluated yet and the different chemical and physical microenvironment of these two hydrogels increase the difficulty of comparison. In this study, three different hydrogels based on collagen and hyaluronic acid (self-assembled collagen hydrogel (Col), self-assembled collagen hydrogel cross-linked with genipin (Cgp), and methacrylated hyaluronic acid hydrogel (HA)) were prepared and their chondro-inductive ability on the encapsulated BMSCs was evaluated. Col and Cgp have the same chemical composition and similar microstructure, but are different from HA, while Cgp and HA hydrogels have the same mechanical strength. It was found that chemical and physical microenvironments of the hydrogels combined to influence cell condensation. Thanks to cell condensation was more likely to occur in collagen hydrogels in the early stage, the cartilage-induced ability was in the order of Col > Cgp > HA. However, the severe shrinkage of Col and Cgp resulted in no enough space for cell proliferation within hydrogels in the later stage. In contrast, relatively stable physical microenvironment of HA helped to maintain continuous production of cartilage-related matrix in the later stage. Overall, these results revealed that the chondro-inductive ability of collagen and hyaluronic acid hydrogel with different chemical and physical microenvironment cannot be evaluated by a particular time period. However, it provided important information for optimization and design of the future hydrogels towards successful repair of articular cartilage.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.05.188DOI Listing
May 2021

Dynamic changes in mitochondrial DNA, morphology, and fission during oogenesis of a seasonal-breeding teleost, Pampus argenteus.

Tissue Cell 2021 May 21;72:101558. Epub 2021 May 21.

College of Marine Science, Ningbo University, Ningbo, China; Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo, China; Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo, China.

Mitochondria play crucial roles during oocyte development. In this study, we have investigated mitochondrial morphology, mtDNA, Ca-ATP enzyme activity, and mitochondrial fission factor (mff) expression levels during oogenesis of the silver pomfret Pampus argenteus. The mtDNA increased with oocyte development, and mitochondrial morphology and distribution were stage-specific. In the perinucleolar oocytes, oval mitochondria were dispersed in the cytoplasm. In previtellogenic oocytes, mitochondria massively increased and aggregated, forming mitochondrial clouds. At the same time, two morphologically different types of mitochondria had been distinguished, one of which was elongated with well-developed cristae, and the other was round with distorted and fused cristae. During vitellogenesis, the increases in mitochondria with well-developed cristae and in Ca-ATPase enzymatic activity were accompanied by an accumulation of yolk substance, suggesting the possible participation of mitochondria in the formation of vitellogenesis. Furthermore, we examined the cDNA of mff its transcript levels in relation to oocyte development. The transcript levels of mff were high in the perinucleolar stage, increasing to the highest level at the previtellogenic stage. Immunocytochemistry showed that MFF was detected in the cytoplasm of previtellogenic and midvitellogenic oocytes. We speculated that the mff-mediated mitochondrial fission may play a crucial role in oocyte development, especially in vitellogenesis.
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http://dx.doi.org/10.1016/j.tice.2021.101558DOI Listing
May 2021

The complete mitogenome and phylogenetic analysis of Indian driftfish, (Scombriformes: Nomeidae).

Mitochondrial DNA B Resour 2021 May 10;6(5):1606-1607. Epub 2021 May 10.

School of Fishery, Zhejiang Ocean University, Zhoushan, China.

The Indian driftfish () is one of the most important commercial fish species in China, Japan and India. The complete mitogenome of was determined in this study. The assembled mitogenome was 16,507 bp and consisted of 13 protein-coding genes, 22 tRNAs, two rRNAs, and a control region. Nucleotide composition of the complete mitogenome was 27.5% A, 28.5% C, 17.5% G, and 26.5% T, with an A + T bias of 53.9%. The maximum-likelihood tree based on 13 protein-coding genes showed that and were the closest to .
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http://dx.doi.org/10.1080/23802359.2021.1914235DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8118399PMC
May 2021

The first determination and analysis of the complete mitochondrial genome of (Siluriformes: Loricariidae).

Mitochondrial DNA B Resour 2021 May 4;6(5):1583-1585. Epub 2021 May 4.

National Engineering Research Center for Marine Aquaculture, Zhejiang Ocean University, Zhoushan, China.

In order to fully comprehend the evolution and kinship of fishes in the family of Loricariidae, the complete mitochondrial genome of the Loricariidae fish was firstly characterized in the present study. The whole mitogenome was 16,657 bp in size and consisted of 13 protein-coding genes, 22 tRNAs, 2 rRNAs genes, a control region and origin of light-strand replication. The proportion of coding sequences with a total length of 11,473 bp was 68.88%, which encoded 3,813 amino acids. The genome composition was highly A + T biased (56.29%), and exhibited AT-skew (0.0661) and a negative GC-skew (-0.2740). All protein-coding genes were started with ATG except for GTG in CO1, while stopped with the standard TAN codons or a single T. The control region (D-loop) ranging from 15,635 bp to 16,657 bp was 1023 bp in size. Until now, there is hardly any studies on the complete mitochondrial sequence in the genus of , phylogenetic analysis showed that was most closely related to in the genus of . The complete mitochondrial genome sequence has provided a new insight into the taxonomic classification, and a more complex picture of the species diversity within the family of Loricariidae.
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http://dx.doi.org/10.1080/23802359.2020.1866446DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8110184PMC
May 2021

Variant haplophasing by long-read sequencing: a new approach to preimplantation genetic testing workups.

Fertil Steril 2021 May 18. Epub 2021 May 18.

Department of Obstetrics and Gynecology, Chinese People's Liberation Army General Hospital, Beijing, People's Republic of China. Electronic address:

Objective: To apply long-read, third-generation sequencing as a part of a general workup strategy for performing structural rearrangement (PGT-SR) and monogenic disease (PGT-M) embryo testing.

Design: Prospective study.

Setting: In vitro fertilization unit.

Patient(s): Couples presenting for PGT-SR (n = 15) and PGT-M (n = 2).

Intervention(s): Blastocyst biopsy with molecular testing for translocation breakpoints or mutations (targets).

Main Outcome Measure(s): Detailed, parental-phased, single-nucleotide polymorphism (SNP) profiles around targets for selection of informative polymorphic markers to simplify and facilitate clinical preimplantation genetic testing (PGT) designs that enable discrimination between carrier and noncarrier embryos.

Result(s): High definition of chromosome breakpoints together with closely phased polymorphic markers was achieved for all 15 couples presenting for PGT-SR. Similarly, for the two couples presenting for PGT-M, tightly linked informative markers around the mutations were also simply identified. Three couples with translocations t(1;17)(q21;p13), t(3;13)(p25;q21.2), and t(12;13)(q23;q22) proceeded with PGT-SR, requesting preferential identification of noncarrier embryos for transfer. Following selection of a set of informative SNPs linked to breakpoints, we successfully performed PGT-SR tests, resulting in ongoing pregnancies with a noncarrier fetus for all couples. Similarly, with the use of tests based on informative SNPs linked to the parental mutations, one couple proceeded with PGT-M for maple syrup urine disease, resulting in an ongoing pregnancy with a disease-free fetus.

Conclusion(s): For couples contemplating clinical PGT, variant haplophasing around the target reduces the workup process by enabling rapid selection of closely linked informative markers for patient-specific test design.
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http://dx.doi.org/10.1016/j.fertnstert.2021.04.012DOI Listing
May 2021

Continuous Fly-Through High-Temperature Synthesis of Nanocatalysts.

Nano Lett 2021 Jun 21;21(11):4517-4523. Epub 2021 May 21.

Department of Materials Science and Engineering, University of Maryland, College Park, Maryland 20742, United States.

The conventional thermal treatment systems typically feature low ramping/cooling rates, which lead to steep thermal gradients that generate inefficient, nonuniform reaction conditions and result in nanoparticle aggregation. Herein, we demonstrate a continuous fly-through material synthesis approach using a novel high-temperature reactor design based on the emerging thermal-shock technology. By facing two sheets of carbon paper with a small distance apart (1-3 mm), uniform and ultrahigh temperatures can be reached up to 3200 K within 50 ms by simply applying a voltage of 15 V. The raw materials can be continuously fed through the device, allowing the final products to be rapidly collected. As a proof-of-concept demonstration, we synthesized Pt nanocatalysts (∼4 nm) anchored on carbon black via this reactor at ∼1400 K. Furthermore, we find it features excellent electrocatalytic activities toward methanol oxidation reaction. This work offers a highly efficient platform for nanomaterials synthesis at high temperatures.
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http://dx.doi.org/10.1021/acs.nanolett.0c03620DOI Listing
June 2021

Mechanism of action of strychni semen for treating Rheumatoid arthritis and methods for attenuating the toxicity.

Comb Chem High Throughput Screen 2021 May 3. Epub 2021 May 3.

Department of Science and Education, Shandong Provincial Third Hospital, 250031, China.

Background: Rheumatoid arthritis (RA) is a chronic autoimmune disease, which destructs joints and causes significant pain, impairing a patient's quality of life. Strychni semen showed promising results in treating RA. However, there are increasing safety concerns in using Strychni Semen due to its severe toxicity.

Aim And Objective: The purpose of this review is to provide insight into using Strychni Semen as an alternative medicine to treat RA, as well as to offer a method for the safe application of Strychni Semen through processing and compatibility studies.

Methods: Publications were retrieved and surveyed from CNKI and PubMed relevant to Strychni Semen for a literature review.

Results: This article summarized the mechanism of function of strychni semen in treating RA with its anti-inflammatory, analgesic, and immunomodulatory effect. Commonly used methods to attenuate the toxicity of strychni semen were also discussed in this article.

Conclusion: Strychni semen has a good therapeutic effect on RA, mainly by the modulation of immunity with anti-inflammatory and analgesic effects. Also, the reported toxicity of strychni semen can be effectively reduced by processing and reasonable compatibility. Hence, as an alternative medicine for RA treatment, strychni semen has a broad prospect.
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http://dx.doi.org/10.2174/1386207324666210504095523DOI Listing
May 2021

Localized tilted fiber Bragg gratings induced by femtosecond laser line-by-line inscription.

Opt Lett 2021 May;46(9):2204-2207

Localized tilted fiber Bragg gratings (TFBGs) with low insertion loss are reported. A series of second-order TFBGs with tilt angles of 0°, 7°, 14°, and 21° was inscribed line by line directly in a single-mode fiber. For the 7° TFBG, the Bragg resonance was 2.4 dB at 1550 nm, and the maximum cladding-mode resonance reached 24.6 dB with an insertion loss of 0.8 dB, the same level as that for TFBGs fabricated by the phase-mask method. The range in cladding-mode resonance for the TFBGs obtained was wider than 170 nm with an intensity exceeding 20 dB. Combined with microscope images, the formation of these localized TFBGs and their spectral performance are discussed. The effect of an inscription offset along the $y$-axis direction was further investigated. With increasing offset, the intensity of the cladding-mode resonance dropped rapidly. The refractive index response sensitivity of the 7° TFBG was measured at 507.54 nm/RIU.
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http://dx.doi.org/10.1364/OL.424581DOI Listing
May 2021

Clinical features and outcomes after endovascular therapy for penetrating aortic ulcer and intramural hematoma.

Vascular 2021 Apr 28:17085381211012573. Epub 2021 Apr 28.

Department of Vascular Surgery, Institute of Vascular Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.

Objectives: To identify the differences between clinical features and outcomes after endovascular therapy for penetrating aortic ulcer (PAU) and intramural hematoma (IMH).

Methods: From January 2009 to March 2020, patients who underwent endovascular therapy for PAU and IMH were enrolled. Information on patient demographics, presentation, PAU and IMH morphology, laboratory examination, and clinical follow-up information was collected and analyzed. Univariate analysis was performed to identify the differences between IMH and PAU, and Kaplan-Meier was used to calculate the cumulative survival rate and freedom from reintervention.

Results: A total of 114 patients were enrolled; 80 (70.2%) of them were diagnosed with PAU. Compared with PAU, patients with IMH were younger ( = 0.006), more likely to be admitted emergently ( = 0.001), had longer hospital stay ( = 0.028), and had higher levels of C-reactive protein ( = 0.030). Meanwhile, patients with IMH were more likely to be associated with hypertension ( = 0.020) and pleural effusion ( < 0.001) and less likely to have a history of acute coronary syndrome ( = 0.019) and prior cardiovascular intervention ( = 0.017). The five-year freedom from reintervention and cumulative survival rate were 94.2% (95% confidential interval, 88.9%-99.9%) and 87.8% (95% confidential interval, 79.5%-96.9%) in PAU patients and 89.6% (95% confidential interval, 75.8%-99.9%) and 85.1% (95% confidential interval, 68.0%-99.9%) in IMH patients, respectively. There was no significant difference in freedom from reintervention ( = 0.795) or cumulative survival rate ( = 0.817).

Conclusions: IMH appeared to occur in younger patients with hypertension and usually had an acute onset, while PAU was more likely to be found incidentally in older patients with atherosclerosis. Endovascular therapy was effective in both IMH and PAU patients with encouraging outcomes.
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http://dx.doi.org/10.1177/17085381211012573DOI Listing
April 2021

Proteomic Exploration of Endocytosis of Framework Nucleic Acids.

Small 2021 Jun 24;17(23):e2100837. Epub 2021 Apr 24.

School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules and National Center for Translational Medicine, Shanghai Jiao Tong University, Shanghai, 200240, China.

Efficient cell internalization of framework nucleic acid nanostructures free of transfection agents provides new opportunities for developing biocompatible and intelligent nanoprobes and drug delivery carriers. Here, a proteomic identification method to screen target proteins that interact with tetrahedral DNA nanostructures (TDNs) during the process of endocytosis by combining drug affinity responsive target stability (DARTS) with liquid chromatography/tandem mass spectrometry (LC-MS/MS) techniques, is reported. It is found that that caveolin-1 (CAV1) and macropinocytosis-related protein sorting nexin5 (SNX5) are associated with the endocytosis of TNDs, which is further validated by microscale thermophoresis (MST) analysis. CAV1- and SNX5- knockout experiments reveal that both caveolae-mediated endocytosis and macropinocytosis mediate the cellular uptake of TDNs, which complement previous findings with fluorescence tracing methods. This method provides a generic strategy to analyze cellular internalization process of DNA nanostructures for biomedical applications.
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http://dx.doi.org/10.1002/smll.202100837DOI Listing
June 2021

MCOLN1/TRPML1 finely controls oncogenic autophagy in cancer by mediating zinc influx.

Autophagy 2021 Apr 23:1-22. Epub 2021 Apr 23.

Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical University, Xuzhou, China.

Macroautophagy/autophagy is elevated to ensure the high demand for nutrients for the growth of cancer cells. Here we demonstrated that MCOLN1/TRPML1 is a pharmaceutical target of oncogenic autophagy in cancers such as pancreatic cancer, breast cancer, gastric cancer, malignant melanoma, and glioma. First, we showed that activating MCOLN1, by increasing expression of the channel or using the MCOLN1 agonists, ML-SA5 or MK6-83, arrests autophagic flux by perturbing fusion between autophagosomes and lysosomes. Second, we demonstrated that MCOLN1 regulates autophagy by mediating the release of zinc from the lysosome to the cytosol. Third, we uncovered that zinc influx through MCOLN1 blocks the interaction between STX17 (syntaxin 17) in the autophagosome and VAMP8 in the lysosome and thereby disrupting the fusion process that is determined by the two SNARE proteins. Furthermore, we demonstrated that zinc influx originating from the extracellular fluid arrests autophagy by the same mechanism as lysosomal zinc, confirming the fundamental function of zinc as a participant in membrane trafficking. Last, we revealed that activating MCOLN1 with the agonists, ML-SA5 or MK6-83, triggers cell death of a number of cancer cells by evoking autophagic arrest and subsequent apoptotic response and cell cycle arrest, with little or no effect observed on normal cells. Consistent with the results, administration of ML-SA5 in Patu 8988 t xenograft mice profoundly suppresses tumor growth and improves survival. These results establish that a lysosomal cation channel, MCOLN1, finely controls oncogenic autophagy in cancer by mediating zinc influx into the cytosol.
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http://dx.doi.org/10.1080/15548627.2021.1917132DOI Listing
April 2021

Mild depolymerization of the sinocalamus oldhami alkali lignin to phenolic monomer with base and activated carbon supported nickel-tungsten carbide catalyst composite system.

Bioresour Technol 2021 Aug 6;333:125136. Epub 2021 Apr 6.

Fujian Provincial Engineering Research Center of Rural Waste Recycling Technology, College of Environment and Resources, Fuzhou University, Fuzhou 350116, Fujian, China; Fujian Provincial Technology Exploitation Base of Biomass Resources, Fuzhou University, Fuzhou 350116, China. Electronic address:

In this study, the sinocalamus oldhami alkali lignin was depolymerized into phenolic products in a combined system by using the composite alkali and Ni-WC/activated carbon (AC) as catalysts. FT-IR, GPC, TG, 2D-HSQC and GC-MS were used to analyze the composition, structure and distribution of degradation products, and the synergistic effect of metal and alkali catalysts on the depolymerization of lignin was also studied. The results showed that Ni-WC/AC and composite alkali could effectively improve the catalytic degradation efficiency of lignin under mild conditions, 94.4% of lignin was converted and 17.18% of phenolic monomers were obtained under 260 °C for 5 h. In this composite system, the synergism of the basic sites, the metal active sites and the Lewis acid sites could promote the cleavage of C-O bonds in the lignin molecule and lower the char formation during the base-catalyzed solvolysis. Phenolic monomers were mainly composed of phenol, 2-methyl-phenol and p-cresol etc.
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http://dx.doi.org/10.1016/j.biortech.2021.125136DOI Listing
August 2021

Current perspectives on exosomes in the diagnosis and treatment of hepatocellular carcinoma (review).

Cancer Biol Ther 2021 Apr 13;22(4):279-290. Epub 2021 Apr 13.

Department of Hepatobiliary Surgery, General Hospital, Shijiazhuang, Hebei, P. R. China.

The prognosis of hepatocellular carcinoma (HCC), a malignant tumor, is poor. Tumor recurrence and metastasis are the major challenges for the treatment of HCC. Various studies have demonstrated that exosomes, which are loaded with various biomolecules including nucleic acids, lipids, and proteins are involved in the recurrence and metastasis of HCC. Additionally, exosomes mediate various biological processes, such as immune response, cell apoptosis, angiogenesis, thrombosis, autophagy, and intercellular signal transduction. In cancer, exosomes regulate cancer cell differentiation, development, and drug resistance. Circular RNAs, microRNAs, and proteins in the exosomes can serve as early diagnostic and prognostic markers for HCC. As exosomes are characterized by low immunogenicity and high stability in the tissues and circulation, they can be used to deliver the drugs in cancer therapies.
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http://dx.doi.org/10.1080/15384047.2021.1898728DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183537PMC
April 2021

Methods Used and Application of the Mouse Grimace Scale in Biomedical Research 10 Years on: A Scoping Review.

Animals (Basel) 2021 Mar 3;11(3). Epub 2021 Mar 3.

JBI, Faculty of Health and Medical Sciences, The University of Adelaide, Adelaide 5005, Australia.

The Mouse Grimace Scale (MGS) was developed 10 years ago as a method for assessing pain through the characterisation of changes in five facial features or action units. The strength of the technique is that it is proposed to be a measure of spontaneous or non-evoked pain. The time is opportune to map all of the research into the MGS, with a particular focus on the methods used and the technique's utility across a range of mouse models. A comprehensive scoping review of the academic literature was performed. A total of 48 articles met our inclusion criteria and were included in this review. The MGS has been employed mainly in the evaluation of acute pain, particularly in the pain and neuroscience research fields. There has, however, been use of the technique in a wide range of fields, and based on limited study it does appear to have utility for pain assessment across a spectrum of animal models. Use of the method allows the detection of pain of a longer duration, up to a month post initial insult. There has been less use of the technique using real-time methods and this is an area in need of further research.
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http://dx.doi.org/10.3390/ani11030673DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999303PMC
March 2021

Effective Antibacterial Activity of Degradable Copper-Doped Phosphate-Based Glass Nanozymes.

ACS Appl Mater Interfaces 2021 Mar 8;13(10):11631-11645. Epub 2021 Mar 8.

Laboratory of Functional and Biomedical Nanomaterials, College of Materials Science and Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.

Copper-containing antimicrobials are highly valuable in the field of medical disinfectants owing to their well-known high antimicrobial efficacy. Artificially synthesized nanozymes which can increase the level of reactive oxygen species (ROS) in the bacterial system have become research hotspots. Herein, we describe the design and fabrication of degradable Cu-doped phosphate-based glass (Cu-PBG) nanozyme, which can achieve excellent antibacterial effects against Gram-positive and Gram-negative bacteria. The antibacterial mechanism is based on the generation of ROS storm and the release of copper. It behaves like a peroxidase in wounds which are acidic and exerts lethal oxidative stress on bacteria catalyzing the decomposition of HO into hydroxyl radicals (OH). Quite different from any other reported nanozymes, the Cu-PBG is intrinsically degradable due to its phosphate glass nature. It gradually degrades and releases copper ions in a physiological environment, which further enhances the inhibition efficiency. Satisfactory antibacterial effects are verified both and . Being biodegradable, the prepared Cu-PBG exhibits excellent biocompatibility and does not cause any adverse effects caused by its long-time residence time in living organisms. Collectively, these results indicate that the Cu-PBG nanozyme could be used as an efficient copper-containing antimicrobial with great potential for clinical translation.
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http://dx.doi.org/10.1021/acsami.0c22746DOI Listing
March 2021

Preparation of a Novel Cellulose-Styrene Copolymer Adsorbent and Its Adsorption of Nitrobenzene from Aqueous Solutions.

Polymers (Basel) 2021 Feb 18;13(4). Epub 2021 Feb 18.

College of Chemical Engineering, Fuzhou University, Fuzhou 350116, China.

A novel cellulose-styrene copolymer adsorbent (cellulose-St) was prepared using free radical polymerization. Successful polymerization was confirmed through Fourier Transform Infrared Spectroscopy (FTIR), Carbon 13 Solid Nuclear Magnetic Resonance (C NMR) Spectroscopy, Scanning Electron Microscopy (SEM), etc. Cellulose-St possessed good hydrophobicity, and the best water contact angle of cellulose-St samples could reach 146°. It had the ability of adsorption for nitrobenzene (NB), and the adsorption process could be well described by the pseudo-second-order (R > 0.99) and three-stage intraparticle diffusion (R > 0.99) kinetic models. Furthermore, the dynamic adsorption experiments revealed that cellulose-St had the potential for continuous separation of NB in water, and the breakthrough point for the initial NB concentration of 10 mg/L reached 1.275 L/g. Moreover, cellulose-St exhibited excellent environmental adaptability that it could maintain its hydrophobicity and adsorption ability for NB in strong acids, strong alkalis, or organic solvents. The used cellulose-St could be reused after washing with ethanol and keep almost constant adsorption capacity after ten cycles.
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http://dx.doi.org/10.3390/polym13040609DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7922655PMC
February 2021

Role of chemokines in hepatocellular carcinoma (Review).

Oncol Rep 2021 03 22;45(3):809-823. Epub 2020 Dec 22.

Department of Hepatobiliary Surgery, Hebei General Hospital, Shijiazhuang, Hebei 050051, P.R. China.

Hepatocellular carcinoma (HCC) is a prevalent malignant tumor worldwide, with an unsatisfactory prognosis, although treatments are improving. One of the main challenges for the treatment of HCC is the prevention or management of recurrence and metastasis of HCC. It has been found that chemokines and their receptors serve a pivotal role in HCC progression. In the present review, the literature on the multifactorial roles of exosomes in HCC from PubMed, Cochrane library and Embase were obtained, with a specific focus on the functions and mechanisms of chemokines in HCC. To date, >50 chemokines have been found, which can be divided into four families: CXC, CX3C, CC and XC, according to the different positions of the conserved N‑terminal cysteine residues. Chemokines are involved in the inflammatory response, tumor immune response, proliferation, invasion and metastasis via modulation of various signaling pathways. Thus, chemokines and their receptors directly or indirectly shape the tumor cell microenvironment, and regulate the biological behavior of the tumor. In addition, the potential application of chemokines in chemotaxis of exosomes as drug vehicles is discussed. Exosomes containing chemokines or expressing receptors for chemokines may improve chemotaxis to HCC and may thus be exploited for targeted drug delivery.
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http://dx.doi.org/10.3892/or.2020.7906DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859922PMC
March 2021

Application of quantum dots in perovskite solar cells.

Nanotechnology 2021 Mar 1. Epub 2021 Mar 1.

Taiyuan University of Technology, Taiyuan, Shanxi , CHINA.

Perovskite solar cells (PSCs) are important candidates for next generation thin-film photovoltaic technology due to their superior performance in energy harvesting. At present, their photoelectric conversion efficiencies (PCEs) are comparable to those of silicon-based solar cells. PSCs have usually multi-layer structure. Therefore, they face the problem that the energy levels between adjacent layers often mismatch to each other. Meanwhile, large amount of defects are often introduced due to the solution preparation procedures. What's more, the perovskite is prone to degradation under ultraviolet (UV) irradiation. These problems could degrade the efficiency and stability of PSCs. In order to solve these problems, quantum dots (QDs), a kind of low-dimensional semiconductor material, have been recently introduced into PSCs as charge transport materials, interfacial modification materials, dopants and luminescent down shifting materials. By these strategies, the energy alignment and interfacial conditions are improved, the defects are efficiently passivated, and the instability of perovskite under UV irradiation is suppressed. So the device efficiency and stability are both improved. In this paper, we overview the recent progress of QDs' utilizations in PSCs.
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http://dx.doi.org/10.1088/1361-6528/abead9DOI Listing
March 2021

A key role of inner-cation-π interaction in adsorption of Pb(II) on carbon nanotubes: Experimental and DFT studies.

J Hazard Mater 2021 06 21;412:125187. Epub 2021 Jan 21.

Department of Environmental Science, School of Geography and Tourism, Shaanxi Normal University, Xi'an 710119, China; International Joint Research Centre of Shaanxi Province for Pollutants Exposure and Eco-environmental Health, Xi'an 710119, China.

Herein the adsorption and desorption of Pb on oxidized (O-CNTs) and graphitized multi-walled carbon nanotubes (G-CNTs) were studied, and detailed adsorption mechanisms were discussed by experimental characterization and density functional theory (DFT) calculation. The adsorption of Pb on CNTs was co-guided by complexation, ion exchange, electrostatic and cation-π interactions. According to the abnormally low release ratio of Pb on both O-CNTs and G-CNTs (<9.03%), the O-containing groups on CNTs surface are not the only key factor affecting the adsorption behavior. The pore filling and complexation are the main mechanisms leading to irreversible adsorption, especially the important role of the inner-cation-π interaction in Pb adsorption into the inner channel of CNTs at the high initial Pb concentration, and DFT calculations further confirmed this result. The adsorption energy of the inner-cation-π interaction between Pb and CNTs can be as high as - 77.851 kJ/mol, which is much higher than other interactions (≤-41.488 kJ/mol). Moreover, the stability of various adsorption mechanisms by HOMO-LUMO energy gap (E), electronic chemical potential (µ) and global hardness (η) were quantitatively measured and further revealed the inner-cation-π interaction is more stable. This study provides a deeper understanding of the removal of heavy metals by porous carbon-based nanomaterials.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125187DOI Listing
June 2021

Prevalence of precocious puberty among Chinese children: a school population-based study.

Endocrine 2021 May 2;72(2):573-581. Epub 2021 Feb 2.

Department of Clinical Epidemiology and Biostatistics, Children Health Advocacy Institute, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Purpose: To investigate the prevalence of precocious puberty in school-based population in Zhongshan, Guangdong, China.

Methods: We recruited a total of 4058 students in grades 1-3 using a multistage stratified cluster random sampling method in a population-based cross-sectional study. We evaluated height, weight, and development of breast and pubic hair in girls, breast development was evaluated by inspection and palpation combined with ultrasound detection for overweight and obese girls, and testicular volume and development of pubic hair in boys. We estimated overall, sex-, age, and district-specific prevalence of precocious puberty and examined the association between prevalence of precocious puberty and body mass index (BMI).

Results: The unadjusted and adjusted prevalence of precocious puberty by Tanner stage was 4.74% (girls:8.78%, boys:2.58%) and 6.19% (girls:11.47%, boys:3.26%), respectively. In both urban and suburban areas, the prevalence of precocious puberty was higher in the overweight and obese group than in the normal-weight group (p < 0.05). The prevalence of precocious puberty among overweight (27.94%) and obese (48.00%) girls was higher than that of normal-weight girls (8.73%) (p value for trend < 0.05). In boys, the prevalence of precocious puberty in the obese (6.78%) group was higher than that in the normal-weight (2.86%) group (p < 0.05).

Conclusions: The prevalence of precocious puberty was high in China. Overweight and obesity was related to precocious puberty, but this correlation had gender differences and may be affected by other environmental factors.
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http://dx.doi.org/10.1007/s12020-021-02630-3DOI Listing
May 2021

Preparation of CeZrO/SnInS composite photocatalyst and its degradation of typical antibiotic pollutants.

Environ Sci Pollut Res Int 2021 Jun 1;28(22):28024-28037. Epub 2021 Feb 1.

Research Institute of Photocatalysis, College of Environment and Resources, Fuzhou University, Fuzhou, 350116, China.

Considering the high environmental risk, the remediation of antibiotic pollutants attracted numerous attentions. In this work, a novel photocatalyst, CeZrO/SnInS, was fabricated by in situ precipitation and hydrothermal method and then applied to the degradation of norfloxacin under the irritation of visible light. The SEM, TEM, XRD, XPS, and electrochemical results clearly showed that the n-type heterojunction between CeZrO and SnInS was successfully constructed, which greatly reduces the recombination of the photogenic electron and holes, leading to the improvement of photocatalytic performance and stability (recycled over eight times). Besides, the CeZrO/SnInS composite also exhibited good ability to mineralize norfloxacin. Under the optimal condition (pH 3, 1 g L of 10% CeZrO/SnInS, and 8 mg L of initial norfloxacin concentration), norfloxacin could be fully and rapidly degraded in 60 min, and completely mineralized in 4 h (99.3 ± 1.7%). LC-QTOF-MS results evidently displayed eight intermediates during norfloxacin degradation. In addition, with the attack of the reactive oxygen species (h, •OH, and •O), norfloxacin could be effectively decomposed via deoxygenation, hydroxylation, and carboxylation reactions. Notably, compared to photodegradation, the photocatalytic process could completely eliminate the norfloxacin from water because it could avoid the accumulation of toxic byproducts.
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http://dx.doi.org/10.1007/s11356-021-12610-9DOI Listing
June 2021

Identifying Signatures of Selection Related to Comb Development.

J Poult Sci 2021 Jan;58(1):5-11

Poultry Institute, Chinese Academy of Agricultural Sciences, Cangjie road, number 58, Yangzhou, 225125, China.

The aim of this study was to identify genes involved in comb development to provide insights into the molecular mechanism of chickens' comb formation. Fixation index (F) and average number of base differences () of males with large and small combs were calculated based on whole-genome resequencing data. Chromosome regions with larger F values and smaller were considered candidate selection regions. Through further annotation of gene functions and pathways, we sought to screen possible selected genes associated with comb development. By screening whole genome resequencing data, F and were calculated using a 40 Kb sliding window strategy and eight regions were identified. Quantitative trait loci (QTL; gene) related to comb length were found on chromosome 1. QTL (, and genes) related to comb weight were found on chromosome 3. QTL (, and genes) associated with comb area were found on the Z chromosome. Nineteen genes, Wnt signaling pathway and neuroactive ligand-receptor interaction signaling pathway directly or indirectly related to comb growth and development were found through functional annotation and GO analysis. Among the selected genes and Wnt signaling pathways were related to immunity. and neuroactive ligand receptor interaction signaling pathways related to neural function were screened. , and genes were related to heat dissipation. Among the selected genes , and associated with immunity, neurological function, and heat dissipation function coincided with genes affecting the length, weight, and area of the comb. Comprehensive analysis suggested that comb development was due to multiple genes and signaling pathways.
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http://dx.doi.org/10.2141/jpsa.0190104DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837803PMC
January 2021

Novel gene rearrangement in the mitochondrial genome of Muraenesox cinereus and the phylogenetic relationship of Anguilliformes.

Sci Rep 2021 Jan 28;11(1):2411. Epub 2021 Jan 28.

National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization, Zhejiang Ocean University, No. 1, Haida South Road, Zhoushan, Zhejiang, 316022, People's Republic of China.

The structure and gene sequence of the fish mitochondrial genome are generally considered to be conservative. However, two types of gene arrangements are found in the mitochondrial genome of Anguilliformes. In this paper, we report a complete mitogenome of Muraenesox cinereus (Anguilliformes: Muraenesocidae) with rearrangement phenomenon. The total length of the M. cinereus mitogenome was 17,673 bp, and it contained 13 protein-coding genes, two ribosomal RNAs, 22 transfer RNA genes, and two identical control regions (CRs). The mitochondrial genome of M. cinereus was obviously rearranged compared with the mitochondria of typical vertebrates. The genes ND6 and the conjoint trnE were translocated to the location between trnT and trnP, and one of the duplicated CR was translocated to the upstream of the ND6. The tandem duplication and random loss is most suitable for explaining this mitochondrial gene rearrangement. The Anguilliformes phylogenetic tree constructed based on the whole mitochondrial genome well supports Congridae non-monophyly. These results provide a basis for the future Anguilliformes mitochondrial gene arrangement characteristics and further phylogenetic research.
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http://dx.doi.org/10.1038/s41598-021-81622-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844273PMC
January 2021

The complete mitochondrial genome of (Characiformes:Nematobrycon) and phylogenetic studies of Characidaes.

Mitochondrial DNA B Resour 2020 Oct 5;5(3):3474-3475. Epub 2020 Oct 5.

National Engineering Research Center for Marine Aquaculture, Zhejiang Ocean University, Zhoushan, China.

Complete mitochondrial genome of the Characiform fish was characterized in the present study. The whole mitogenome was 17,340 bp in size and the proportion of coding sequences with a total length of 11,448 bp was 66.02%, which encodes 3805 amino acids. The base composition of the genome was 30.92% for A, 23.92% for C, 14.88% for G, and 30.28% for T. All protein-coding genes were started with ATG, CO1 and ATP8 ended by AGG, TAG respectively, whereas CO2, ATP6, ND4 ended by a single T, the other PCGs commonly ended by TAA. The length of 12S and 16S ribosomal RNA was 949 bp and 1675 bp, respectively. The control region (D-loop) ranging from 15,654 bp to 17,340 bp was 1687 bp in size. It showed negative GC skew value (-0.2329) and positive AT skewness (0.0105). Phylogenetic analysis showed that was most closely related to . The complete mitochondrial genome sequence would provide a new insight into taxonomic classification, and help to draw a more complete picture of species diversity within the Characidae.
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http://dx.doi.org/10.1080/23802359.2020.1825130DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782840PMC
October 2020

Characterization of the complete mitochondrial genome of Chinese (Clupeiformes, Clupeidae) and phylogenetic studies of Clupeiformes.

Mitochondrial DNA B Resour 2020 Sep 18;5(3):3371-3373. Epub 2020 Sep 18.

Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, China.

The Dotted Gizzard Shad () was one of the most important commercial fish species in China, Japan and Korea. In this study, the complete mitochondrial genome of was presented. The full length of the mitochondrial genome was 16,705 bp, including 13 protein-coding genes (PCGs), two ribosomal RNAs, 22 transfer RNA genes, one non-coding control region (CR) and one origin of replication on the light-strand. The total nucleotide composition of mitochondrial DNA was 25.79%A, 25.09%T, 29.05%C, 20.08%G, and AT was 50.88%. The mitochondrial genome provides an important resource for solving taxonomic problems and studying molecular evolution.
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http://dx.doi.org/10.1080/23802359.2020.1823272DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782019PMC
September 2020

Supramolecular nanofibers increase the efficacy of 10-hydroxycamptothecin by enhancing nuclear accumulation and depleting cellular ATP.

Acta Biomater 2021 03 11;122:343-353. Epub 2021 Jan 11.

Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, 300192, China; College of Materials Science and Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China. Electronic address:

Poor nuclear delivery and accumulation are the main reasons for the reduced drug efficacy of many anticancer drugs that target DNA or enzymes in the nucleus, and it is a major obstacle to successful cancer therapy. To address this problem, developing practical drug delivery systems for nuclear delivery is urgently needed. Here we develop a supramolecular hydrogel by conjugating the anticancer agent 10-hydroxycamptothecine (HCPT) and macrocyclic polyamine cyclen to a self-assembling peptide. The cyclen fragment possesses nuclear localization and ATP hydrolysis properties, which can provide a synergistic therapeutic effect for cancer treatment. The HCPT-FFFK-cyclen nanofibers showed improved nuclear accumulation and inhibition capacity in cancer cells including drug-resistant cancer cells in vitro. The nanofibers also exhibited favorable ATP consuming ability in vitro. Moreover, the obtained nanomedicine showed enhanced anticancer efficiency and favorable biocompatibility in vivo when administered to mice via tail vein injection. This constructed self-delivery drug system significantly improved the delivery efficiency of the small molecule agents into the nucleus and showed favorable ATP consuming ability, offering new strategies for developing nanomedicines for cancer combination therapy.
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http://dx.doi.org/10.1016/j.actbio.2020.12.052DOI Listing
March 2021

Characterization of the complete mitochondrial genome of Macrotocinclus affinis (Siluriformes; Loricariidae) and phylogenetic studies of Siluriformes.

Mol Biol Rep 2021 Jan 13;48(1):677-689. Epub 2021 Jan 13.

National engineering Laboratory of Marine Germplasm Resources Exploration and Utilization, Zhejiang Ocean University, Zhoushan, 316022, China.

The vertebrate mitochondrial genome is typically circular molecules made up of 14,000 to 16,000 bp, including 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (12 s rRNA and 16 s rRNA) and a control region. Compared with nuclear DNA, mitochondrial DNA has a higher mutation rate, so it is one of the most effective and reliable molecular markers in fish phylogeny. Macrotocinclus affinis was the only species in Macrotocinclus (it was classified as Otocinclus in the past) and currently lacks genetic information. Most of the current researches are based on the mitochondrial Cytb gene and RAG1 and RAG2 nuclear genes to study the phylogenetic analysis of Siluriformes. So, the study provides the characteristic features of the Macrotocinclus affinis mitochondrial genome and this is the first time that the phylogenetic relationship of Siluriformes has been reconstructed based on COI. We aimed to sequence the entire mitochondrial genome of Macrotocinclus affinis using conventional PCR techniques and to clarify its phylogenetic status in Siluriformes by using the COI sequence of mitochondria. In this study, we sequenced the whole mitochondrial genome of this species yielding a 16,632 bp circular assembly composed of the typical vertebrate mitochondrial features. It contains 13 protein-coding genes, two rRNA genes, 22 tRNA genes, a putative control region, and one origin of replication on the light-strand. The overall base composition includes A (30.07%), T (24.43%), C (29.43%) and G (16.01%). The genome composition is A + T biased (54.5%), and exhibits AT-skew (0.1036) and GC-skew (-0.2962). Moreover, the 13 PCGs encode 3850 amino acids in total. The result of the phylogenetic tree supports Macrotocinclus affinis has a closest relationship with Otocinclus cf. hoppei far. These results will help to understand the characteristics of the mitochondrial genome of Macrotocinclus affinis and provide molecular basis for the evolutionary relationship of Loricariidae.
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http://dx.doi.org/10.1007/s11033-020-06120-zDOI Listing
January 2021

Rapidly photocatalytic mineralization of typical veterinary drugs with the SnO/SnInS composite.

Chemosphere 2021 May 28;271:129452. Epub 2020 Dec 28.

Research Institute of Photocatalysis, College of Environment & Resources, Fuzhou University, Fuzhou, 350116, China. Electronic address:

Considering the high environmental risk, the remediation of veterinary drug pollutants aroused numerous concerning. In this paper, a novel photocatlyst, SnO/SnInS, was fabricated by in situ precipitation and hydrothermal method and then employed to simulate photocatalytic degradation of olaquindox under visible light. The SEM, TEM, XRD, XPS and electrochemical results clearly showed that the n-type heterojunction between SnO and SnInS was successfully constructed, which greatly reduce the recombination of the photogenic electron and holes, leading to the improvement of photocalytic performance and stability (recycled over 10 times). Besides, the SnO/SnInS composite also exhibited good ability to mineralize the olaquindox. Under the optimal condition (pH of 3, 1 g L of 30 wt% SnO/SnInS and 10 mg L of initial olaquindox concentration), the olaquindox could be fully and rapidly degraded in 25 min, and completely mineralized in 2 h (99.3 ± 1.7%). LC-QTOF-MS analysis evidently displayed 10 intermediates during the olaquindox degradation. In addition, with the attack of the reactive oxygen species (h, •OH and •O), olaquindox could be effectively decomposed via deoxygenation, hydroxylation and carboxylation reactions. Importantly, compared to photodegradation, the photocatalytic process was an ideal way to eliminate the olaquindox form water because it could avoid the accumulation of toxic byproducts.
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http://dx.doi.org/10.1016/j.chemosphere.2020.129452DOI Listing
May 2021