Publications by authors named "Yicheng Yang"

35 Publications

Expansion Microscopy with Multifunctional Polymer Dots.

Adv Mater 2021 May 14:e2007854. Epub 2021 May 14.

Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong, 518055, China.

Expansion microscopy (ExM) provides nanoscale resolution on conventional microscopes via physically enlarging specimens with swellable polyelectrolyte gels. However, challenges involving fluorophore degradation and dilution during sample expansion have yet to be overcome. Herein, sequential cellular targeting, gel anchoring, and high-fidelity fluorescence reported using multifunctional polymer dots (Pdots) designed for ExM applications are demonstrated. The impressive brightness of the Pdots facilitates multicolor ExM, thereby enabling visualization of a variety of subcellular structures and neuron synapses. The average fluorescence intensities of Pdots in ExM range from ≈3 to 6 times higher than those achieved using commercially available Alexa dyes. Moreover, the fluorescence brightness and optical fluctuation are significantly improved by a surfactant-containing expansion buffer, which enables further resolution enhancement via super-resolution optical fluctuation imaging (SOFI). The combination of ExM and SOFI allows subcellular structures of ≈30 nm to be resolved by conventional microscopes. These results highlight the immense potential of multifunctional Pdots for ExM-enhanced super-resolution imaging.
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http://dx.doi.org/10.1002/adma.202007854DOI Listing
May 2021

Molecular survey of Babesia spp. in red foxes (Vulpes Vulpes), Asian badgers (Meles leucurus) and their ticks in China.

Ticks Tick Borne Dis 2021 Mar 17;12(4):101710. Epub 2021 Mar 17.

School of Animal Science and Technology, Shihezi University, Shihezi City, Xinjiang Uygur Autonomous Region, 832000, People's Republic of China. Electronic address:

Babesia species (Apicomplexa: Piroplasmorida) are tick-borne protozoan hemoparasites, which pose a significant threat to domestic animals, wildlife and humans. This study aimed to determine and characterize Babesia species in red foxes (Vulpes vulpes), Asian badgers (Meles leucurus) and their ticks. Blood, heart, liver, spleen, lung, kidney, large intestine and small intestine were collected from 19 wild carnivores (12 red foxes and 7 Asian badgers). All ticks were removed from these animals and identified according to morphological and molecular characteristics. The samples were tested for the presence of Babesia species using the 18S rRNA gene. Molecular analyses showed that the DNA of Babesia vogeli and Babesia vulpes was present in red fox organs/tissues and blood samples. A total of 54 hard ticks (38 Ixodes canisuga, 6 Haemaphysalis erinacei, 9 Ixodes kaiseri and 1 Dermacentor marginatus) were collected from red foxes and 12 (I. kaiseri) from Asian badgers. All ticks were adults. Among them, one I. kaiseri parasiting a red fox contained the DNA of B. vulpes while one I. canisuga was positive for Babesia sp. belonging to the clade "Babesia sensu stricto". Molecular and phylogenetic analyses indicated the presence of a novel genotype, Babesia sp. "badger China". Babesia sp. badger type A and type B from Asian badgers were different from those in European badgers. Co-infection with three Babesia genotypes was found in one Asian badger. This study provides the first data on Babesia infection in red foxes, Asian badgers and their ticks in China. Babesia vogeli was detected for the first time in red foxes in Asia. Co-infection and genetic diversity of Babesia genotypes in Asian badgers were also demonstrated.
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http://dx.doi.org/10.1016/j.ttbdis.2021.101710DOI Listing
March 2021

Mildly Elevated Pulmonary Arterial Pressure Is Associated With a High Risk of Progression to Pulmonary Hypertension and Increased Mortality: A Systematic Review and Meta-Analysis.

J Am Heart Assoc 2021 Apr 23;10(7):e018374. Epub 2021 Mar 23.

Department of Cardiology Center for Diagnosis and Management of Pulmonary Vascular Diseases Fuwai Hospital National Center for Cardiovascular Diseases Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China.

Background Pulmonary hypertension (PH) is defined as a mean pulmonary arterial pressure (PAP) ≥25 mm Hg measured by right heart catheterization. However, the upper limit of a normal mean PAP is 20 mm Hg. There is a gap between the upper limit of normal and the threshold for diagnosing PH. Therefore, we aimed to investigate whether individuals with a mildly elevated PAP, defined as 20 mm Hg < mean PAP <25 mm Hg, are at an increased risk of progression to PH or mortality than those with a normal PAP. Methods and Results We reviewed studies evaluating the risk of progression to PH and/or mortality in individuals with a mildly elevated PAP versus those with a normal PAP. The mean PAP value of each participant was confirmed by right heart catheterization. We reviewed 1213 studies and 8 fulfilled our inclusion criteria. Our results indicated that individuals with a mildly elevated PAP were 1.81 to 2.45 times more likely to progress to PH than individuals with a normal PAP. There was a statistically significant difference in mortality between the mildly elevated PAP and normal PAP groups (hazard ratio, 2.48; 95% CI, 1.69-3.64). We also pooled survival probabilities in each arm to obtain a summary survival curve for each group, and the pooled survival rates in the mildly elevated PAP group were numerically lower than those in the normal PAP group. Conclusions Our study revealed that individuals with a mildly elevated PAP were at an increased risk of progression to PH and mortality than those with a normal PAP.
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http://dx.doi.org/10.1161/JAHA.120.018374DOI Listing
April 2021

ZnO/biochar nanocomposites via solvent free ball milling for enhanced adsorption and photocatalytic degradation of methylene blue.

J Hazard Mater 2021 Mar 6;415:125511. Epub 2021 Mar 6.

Department of Agricultural and Biological Engineering, University of Florida, Gainesville, FL 32611, United States.

There are challenges in developing multifunctional materials that can not only effectively adsorb but also completely eliminate organic contaminants in water. In this work, novel ZnO/biochar nanocomposites were synthesized using a facile ball-milling method. A series of characterization results showed that the ZnO nanoparticles dispersed uniformly on carbon surface within the biochar matrix. Ball milling increased the mesopores and macropores of the nanocomposites by breaking biochar and squeezing ZnO. The addition of appropriate amount of ZnO into biochar enhanced both the adsorption capacity and photocatalytic ability of the nanocomposites for methylene blue (MB) removal. When the initial concentration of MB was 160 mg/g, the nanocomposites exhibited high MB removal efficiency (up to 95.19%) under visible light through the combination of adsorption and photocatalysis. This work provides a feasible synthesis of metal oxide/biochar nanocomposites with excellent adsorption and photocatalysis properties for the treatment of organic dye wastewater.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125511DOI Listing
March 2021

Genetic Diversity of Hard Ticks (Acari: Ixodidae) in the South and East Regions of Kazakhstan and Northwestern China.

Korean J Parasitol 2021 Feb 19;59(1):103-108. Epub 2021 Feb 19.

Department of Basic Medicine, School of Medicine, Shihezi University, Shihezi City, Xinjiang Uygur Autonomous Region 832002, People's Republic of China.

To date, there is no report on the genetic diversity of ticks in these regions. A total of 370 representative ticks from the south and east regions of Kazakhstan (SERK) and Xinjiang Uygur Autonomous Region (XUAR) were selected for molecular comparison. A fragment of the mitochondrial cytochrome c oxidase subunit I (cox1) gene, ranging from 631 bp to 889 bp, was used to analyze genetic diversity among these ticks. Phylogenetic analyses indicated 7 tick species including Hyalomma asiaticum, Hyalomma detritum, Hyalomma anatolicum, Dermacentor marginatus, Rhipicephalus sanguineus, Rhipicephalus turanicus and Haemaphysalis erinacei from the SERK clustered together with conspecific ticks from the XUAR. The network diagram of haplotypes showed that i) Hy. asiaticum from Almaty and Kyzylorda Oblasts together with that from Yuli County of XUAR constituted haplogroup H-2, and the lineage from Chimkent City of South Kazakhstan was newly evolved; and ii) the R. turanicus ticks sampled in Israel, Almaty, South Kazakhstan, Usu City, Ulugqat and Baicheng Counties of XUAR were derivated from an old lineage in Alataw City of XUAR. These findings indicate that: i) Hy. asiaticum, R. turanicus and Ha. erinacei shared genetic similarities between the SERK and XUAR; and ii) Hy. marginatum and D. reticulatus show differences in their evolution.
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http://dx.doi.org/10.3347/kjp.2021.59.1.103DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7939963PMC
February 2021

Ball milling biochar iron oxide composites for the removal of chromium (Cr(VI)) from water: Performance and mechanisms.

J Hazard Mater 2021 Jul 28;413:125252. Epub 2021 Jan 28.

Department of Agricultural and Biological Engineering, University of Florida, Gainesville, FL 32611, United States.

As the first of its kind, a novel biochar/iron oxide composite (BM-Fe-HC) was successfully prepared by simply ball milling iron-laden biochar (Fe-HC). The performance and mechanisms of Cr(VI) removal by BM-Fe-HC were investigated. Ball milling effectively reduced particle size, increased specific surface area, more importantly, enhanced the distribution and increased the exposure of iron oxides on biochar surface. As a result, Cr(VI) removal by BM-Fe-HC showed fast kinetics and large adsorption capacity with the Langmuir maximum capacity of 48.1 mg/g, higher than that of other biochar/iron composites reported in the literature. Acidic pH promoted Cr(VI) removal while competition ions (Cl, SO and PO) inhibited Cr(VI) removal by BM-Fe-HC. Comparison of pre- and post-adsorption samples revealed that iron oxides of the BM-Fe-HC played the dominant role in the adsorption and reduction of Cr(VI) during the removal. After adsorption, part of adsorbed Cr(VI) was reduced by Fe(II) and then stabilized by Fe(III) in the form of amorphous CrFe(OH) on the composite surface. All the results demonstrate that novel ball-milled biochar/iron oxide composites can be used as an effective adsorbent to remove Cr(VI) from water.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125252DOI Listing
July 2021

Human Tacheng Tick Virus 2 Infection, China, 2019.

Emerg Infect Dis 2021 Feb;27(2):594-598

We used metagenomic analysis to identify Tacheng tick virus 2 infection in a patient with a history of tick bite in northwestern China. We confirmed the virus with reverse transcription-PCR, virus isolation, and genomic analysis. We detected viral RNA in 9.6% of ticks collected from the same region.
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http://dx.doi.org/10.3201/eid2702.191486DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7853585PMC
February 2021

Identification of Potential Risk Genes and the Immune Landscape of Idiopathic Pulmonary Arterial Hypertension via Microarray Gene Expression Dataset Reanalysis.

Genes (Basel) 2021 Jan 19;12(1). Epub 2021 Jan 19.

Pulmonary Vascular Disease Center, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China.

Gene dysfunction and immune cell infiltration play an essential role in the pathogenesis of idiopathic pulmonary arterial hypertension (IPAH). We aimed to investigate the immune landscape and novel differentially expressed genes (DEGs) of IPAH. In addition, potential druggable molecular targets for IPAH were also explored. In this study, the GSE117261 dataset was reanalyzed to explore the immune landscape and hub DEGs of IPAH. Lasso Cox regression analysis and receiver operating characteristic curve analysis were performed to detect the predictive value of IPAH. Additionally, the underlying drug targets for IPAH treatment were determined by drug-gene analysis. IPAH was significantly associated with the transforming growth factor-β (TGF-β) signaling pathway and Wnt signaling pathway as well as energetic metabolism dysfunction. We identified 31 upregulated and 39 downregulated DEGs in IPAH patients. Six hub genes, namely, , , , , , and , were related to IPAH pathogenesis regardless of sex differences. Prediction model analysis showed that the area under the curve values of the hub DEGs except were all above 0.9 for distinguishing IPAH patients. In addition, the relative proportions of 5 subtypes of immune cells, namely, CD T cells, CD memory resting T cells, γ delta T cells, M1 macrophages, and resting mast cells, were significantly upregulated in the IPAH samples, while 6 subtypes of immune cells, namely, CD naive T cells, resting NK cells, monocytes, M0 macrophages, activated mast cells, and neutrophils, were downregulated. Additionally, a total of 17 intersecting drugs targeting 5 genes, , , , , and , were generated as potential druggable molecular targets for IPAH. Our study revealed the underlying correlations between genes and immune cells in IPAH and demonstrated for the first time that , , , , and may be novel genetic targets for IPAH.
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http://dx.doi.org/10.3390/genes12010125DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835985PMC
January 2021

Fluorination Enhances NIR-II Fluorescence of Polymer Dots for Quantitative Brain Tumor Imaging.

Angew Chem Int Ed Engl 2020 11 8;59(47):21049-21057. Epub 2020 Sep 8.

Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong, 518055, China.

Here, we describe a fluorination strategy for semiconducting polymers for the development of highly bright second near-infrared region (NIR-II) probes. Tetrafluorination yielded a fluorescence QY of 3.2 % for the polymer dots (Pdots), over a 3-fold enhancement compared to non-fluorinated counterparts. The fluorescence enhancement was attributable to a nanoscale fluorous effect in the Pdots that maintained the molecular planarity and minimized the structure distortion between the excited state and ground state, thus reducing the nonradiative relaxations. By performing through-skull and through-scalp imaging of the brain vasculature of live mice, we quantitatively analyzed the vascular morphology of transgenic brain tumors in terms of the vessel lengths, vessel branches, and vessel symmetry, which showed statistically significant differences from the wild type animals. The bright NIR-II Pdots obtained through fluorination chemistry provide insightful information for precise diagnosis of the malignancy of the brain tumor.
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http://dx.doi.org/10.1002/anie.202007886DOI Listing
November 2020

SARS-CoV-2: characteristics and current advances in research.

Virol J 2020 07 29;17(1):117. Epub 2020 Jul 29.

Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, 510515, China.

Coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2 infection has spread rapidly across the world and become an international public health emergency. Both SARS-CoV-2 and SARS-CoV belong to subfamily Coronavirinae in the family Coronaviridae of the order Nidovirales and they are classified as the SARS-like species while belong to different cluster. Besides, viral structure, epidemiology characteristics and pathological characteristics are also different. We present a comprehensive survey of the latest coronavirus-SARS-CoV-2-from investigating its origin and evolution alongside SARS-CoV. Meanwhile, pathogenesis, cardiovascular disease in COVID-19 patients, myocardial injury and venous thromboembolism induced by SARS-CoV-2 as well as the treatment methods are summarized in this review.
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http://dx.doi.org/10.1186/s12985-020-01369-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7387805PMC
July 2020

Fe-modified biochar enhances microbial nitrogen removal capability of constructed wetland.

Sci Total Environ 2020 Oct 20;740:139534. Epub 2020 May 20.

State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China. Electronic address:

To improve the nitrogen removal capability of constructed wetlands, the biochar, produced from bamboo, activated with HCl and coated with Fe (FeCl·6HO), and then was added as a substrate into the systems. Three horizontal subsurface flow constructed wetlands (HSCWs) was established to treat the low C/N tailwater from the wastewater treatment plant: C-HSCW (quartz sand + soil), B-HSCW (quartz sand + soil + unmodified biochar), and FeB-HSCW (quartz sand + soil + Fe-modified biochar). Under different combinations of hydraulic retention time and nitrogen loading, the FeB-HSCW revealed extremely effective nitrogen removal, compared to the C-HSCW and B-HSCW. The highest removal efficiencies of NO-N (95.30%), TN (86.68%), NH-N (86.33%), NO-N (79.35%) and COD (63.36%) were obtained in FeB-HSCW with the hydraulic retention time of 96 h. and low influent nitrogen loading (C/N of 2.5). Nitrogen mass balance analysis showed that microbial processes played the most important role of nitrogen removal in HSCWs and the Fe-modified biochar significantly enhanced the microbial nitrogen removal. A total of 128.40 g nitrogen was removed by microorganisms in FeB-HSCW (average removal rate of 2.52 g N/(m·d)), much higher than that in other two HSCWs. The contributions of microorganisms, substrate storage and plant uptake on the total amount of nitrogen removal in the FeB-HSCW was 92.69%, 2.97% and 4.34%, respectively. Moreover, FeB significantly increased the abundances of genes involved in nitrogen removal. The copy numbers of bacterial 16S rRNA and amx, as well as of genes nirS, nirK, nosZ-I, nosZ-II, and hzsA were 1.3- to 27.8-fold higher in the FeB-HSCW than that in the other two HSCWs. Thus, Fe-modified biochar provides a feasible and effective amendment for constructed wetlands to improve the nitrogen removal, particularly nitrate-N, for low C/N wastewaters by enhancing the microbial nitrogen removal capacity (mainly of the denitrification).
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http://dx.doi.org/10.1016/j.scitotenv.2020.139534DOI Listing
October 2020

Investigational pharmacotherapy and immunotherapy of pulmonary arterial hypertension: An update.

Biomed Pharmacother 2020 Sep 16;129:110355. Epub 2020 Jun 16.

State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College. Beijing, 100037, China. Electronic address:

Pulmonary arterial hypertension (PAH) is a progressive disease with limited effective therapies that can be lethal in the terminal stage. Since the universal use of traditional PAH-specific drugs affecting the nitric oxide pathway, endothelin pathway and prostacyclin pathway, major advances to treat this devastating condition have been made. For example, it has been recently demonstrated that PAH is associated with various dysfunctional genes and molecular signaling pathways, abnormal metabolism and endocrine systems, inflammation and immune dysfunction. Numerous descriptions of novel mechanisms in PAH have been presented, and some new drugs targeted to ease, reverse or cure PAH have been developed at the laboratory-level and/or tested in clinical trials. There are also some investigated drugs that have shown promising efficacy and safety in animal models but have failed in human beings. Inspiringly, immunotherapies also seem to be valuable therapies for PAH patients. In this review, we update the advances in investigational pharmacotherapy and immunotherapy of PAH. Hopefully this work can help promote more non-invasive treatment for PAH patients.
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http://dx.doi.org/10.1016/j.biopha.2020.110355DOI Listing
September 2020

Acupuncture at LR3 and KI3 shows a control effect on essential hypertension and targeted action on cerebral regions related to blood pressure regulation: a resting state functional magnetic resonance imaging study.

Acupunct Med 2021 02 12;39(1):53-63. Epub 2020 Jun 12.

School of the Tradition Chinese Medicine, Southern Medical University, Guangzhou, China.

Objective: The aim of this study was to investigate the effects of acupuncture at LR3 and KI3 on hypertension at different time points and on related cerebral regions using resting-state functional magnetic resonance imaging (rs-fMRI).

Methods: We randomly divided 29 subjects into two groups: Group A (receiving acupuncture at LR3 + KI3; 15 subjects) and group B (receiving acupuncture at LR3 and a sham location not corresponding to any traditional acupuncture point; 14 subjects). Acupuncture was performed. Blood pressure (BP) changes were recorded and analyzed using SPSS 20.0 statistical software. We used a 3.0T MRI scanner and standard GE 8 channel head coil to collect whole brain fMRI data in both groups. Data analysis and processing was based on the R2009a MATLAB platform. REST 1.8 software was used to analyze the whole brain amplitude of low-frequency fluctuation (ALFF).

Results: After acupuncture, a statistically significant reduction in BP at different time points was observed in group A. In group B, a statistically significant reduction was found only in diastolic blood pressure (DBP) and was not sustained. Acupuncture at LR3 + KI3 specifically affected brain areas involved in BP regulation, as well as those involved in auditory sense, speech, vision, movement and sensation.

Conclusion: Acupuncture at LR3 + KI3 showed positive immediate and long-term effects on BP, particularly systolic blood pressure (SBP). After ALFF analysis, we concluded that LR3 + KI3 activates brain areas related to BP regulation. In addition, after acupuncture at LR3 + KI3, a highly targeted effect was observed in brain areas associated with BP. In addition, extracerebral areas involving vision, motion control, cognition and hearing were activated, which could potentially contribute to the mitigation of hypertensive complications in patients in an advanced stage of the disease.
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http://dx.doi.org/10.1177/0964528420920282DOI Listing
February 2021

Numerical Simulation and Experimental Research on Temperature Distribution of Fillet Welds.

Materials (Basel) 2020 Mar 9;13(5). Epub 2020 Mar 9.

China Productivity Center for Machinery, China Academy of Machinery Science & Technology, Beijing 100044, China.

In this paper, a matrix equation for the welding heat source model was proposed to calculate the fillet welds temperature distribution based on the penetration depth and molten width. A double ellipsoid heat source model of fillet weld was established firstly by physical experiment and simulation calculation, and then the orthogonal experiment was constructed based on the previous calculation methods and experimentally measured data. Finally, the matrix equation of the heat source model parameters was obtained by regression analysis based on the joint penetration and width. The experimental and numerical simulation of the temperature distribution had been performed for the fillet weld and the results show that (1) the heat flux increases in one direction, while, oppositely, it decreased in another direction; (2) simulation results were highly in accordance with experiments results. The results indicated that the double ellipsoidal heat source model calculated by the matrix equation is quite appropriate for predicting the transient temperature distribution on the fillet welds for the gas metal arc welding process.
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http://dx.doi.org/10.3390/ma13051222DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7085100PMC
March 2020

Bioaccumulations and potential human health risks assessment of heavy metals in ppk-expressing transgenic rice.

Sci Total Environ 2020 Mar 3;710:136496. Epub 2020 Jan 3.

State Key Laboratory of Pollution Control and Resources Reuse, School of the Environment, Nanjing University, Nanjing 210023, PR China. Electronic address:

In order to reduce the phosphorus (P) resource consume, the polyphosphatekinase (ppk)-expressing transgenic rice (ETR) with high utilization efficiency of P fertilizer had been constructed. However, synthesis polyphosphates (polyP) mediated byppkin the plants have the ability of chelating heavy metals, so the potential hazards of the new elite rice variety have raised concerns. In the study, we planted ETR and wild-type Nipponbare (WT) in paddy fields in southern China. After harvest, the concentrations of eight heavy metals in rice tissues were measured, and health risks assessments were performed. The field experiment showed that the ppkexpressions were detected in the roots and straws of ETR plants but did not increase the concentrations of As, Cd, Cr, Ni and Pb in rice tissues. The Hg concentration in the ETRD root was 1.70-fold higher than that in WT, but the abundant Hg bioaccumulation in ETRD only occurred in the root. The bioaccumulation factors (BAFs) of all the detected heavy metals in the ETRS were no different from WT except for Cu and Zn. The results of human health risks assessment of heavy metals in brown rice showed that the non-carcinogenic risks of Cu or Zn in ETRD were higher than that in WT, while there was no difference in the total noncarcinogenic risk of the eight heavy metals in ETR. The carcinogenic risks of heavy metals in ETR were also comparable to that in WT. The results of this study indicated that the ppk expression in rice did not increase human health risks of heavy metals by consuming brown rice, which would provide a safety guarantee for agricultural and environmental applications of ETR not only with single-copy line but also with double-copy line.
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http://dx.doi.org/10.1016/j.scitotenv.2020.136496DOI Listing
March 2020

Experimental and Numerical Investigations on the Thermomechanical Behavior of 304 Stainless Steel/Q345R Composite Plate Weld Joint.

Materials (Basel) 2019 Oct 24;12(21). Epub 2019 Oct 24.

College of Pipeline and Civil Engineering, China University of Petroleum (East China), Qingdao 266580, China.

In this study, the welding process of 304 stainless steel/Q345R low alloy steel composite plate is modeled by experimental and finite element methods to study the complex thermomechanical behavior. The residual stress and microstructure evolution of composite plate in the welding process are also investigated. The welding thermal cycle curve and residual stress distribution at the joint are obtained by using thermocouple and blind-hole methods. Optical microscopy, scanning electron microscopy, and energy-dispersive X-ray spectroscopy were used to investigate the evolution of microstructure, morphology, and element diffusion of the joint. The results show that the maximum von Mises welding residual stress is 312 MPa, which is located in the bottom of the start point of the weld zone. The residual stress gradually decreases and tends to be stable along the direction from the weld to the base metal. In addition, a residual stress discontinuity is found at the interface between the bimetal. It is also found that the closer it is to the weld joint, the more uniform is the austenite distribution and the smaller are the grain sizes.
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http://dx.doi.org/10.3390/ma12213489DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6861911PMC
October 2019

A case with neurological abnormalities caused by Rickettsia raoultii in northwestern China.

BMC Infect Dis 2019 Sep 11;19(1):796. Epub 2019 Sep 11.

School of Medicine, Shihezi University, Shihezi, 832002, Xinjiang Uygur Autonomous Region, China.

Background: The number of new rickettsial species are rapidly increasing, and increasing numbers of Rickettsia raoultii (R. raoultii) infection cases have been detected in humans. However, neurological abnormalities caused by R. raoultii are rarely reported, especially in northwestern China.

Case Presentation: A 36-year-old Kazakh shepherd with an attached tick on part temporalis, presented with right eyelid droop, lethargy, fever, headache, fever (38.0-41.0 °C) and erythematous rash. The examination of cerebrospinal fluid (CSF) showed cerebrospinal pressure of 200 mm HO, leukocyte count of 300.0 × 10/L, adenosine deaminase of 2.15 U/L, and total protein concentration of 0.93 g/L. The diagnosis of R. raoultii infection was confirmed by six genetic markers, and semi-quantified by enzyme-linked immunosorbent assay for rickettsial antigen. The patient gradually recovered after treatment with doxycycline and ceftriaxone. R. raoultii DNA was found both in a tick detached from this patient and in 0.18% (2/1107) of blood samples collected from local shepherds.

Conclusions: This is the first reported case with neurological abnormalities caused by R. raoultii in northwestern China. It is vital to detect rickettsial agents both in blood and CSF for tick bite patients with neurological abnormalities. Public health workers and physicians should pay attention to neurological abnormalities caused by Rickettsia.
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http://dx.doi.org/10.1186/s12879-019-4414-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6737699PMC
September 2019

Environmental occurrences, fate, and impacts of microplastics.

Ecotoxicol Environ Saf 2019 Nov 30;184:109612. Epub 2019 Aug 30.

Department of Agricultural and Biological Engineering, University of Florida, Gainesville, FL, 32611, United States. Electronic address:

Microplastics (MPs) are small plastic pieces with size less than 5 mm that have entered and polluted the environment. While many investigations including several critical reviews on MPs in the environment have been conducted, most of them are focused on their occurrences in marine environment. Current understanding on the occurrences, behaviors, and impacts of MPs in the terrestrial environment is far from complete. A systematic review of the literature was thus conducted to promote the research on MPs in the environment. This work is designed to provide a comprehensive overview that summarizes current knowledge and research findings on environmental occurrences, fate and transport, and impacts of MPs. In addition to discussing the occurrences, characteristics, and sources of MPs in the ocean, freshwater, sediments, soils, and atmosphere, the review also summarizes both the experimental and modeling data of the environmental fate and transport of MPs. Research findings on the toxic effects, bioaccumulation, and bioavailability of MPs in the environment are also covered in this critical review. Future perspectives are discussed as well.
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http://dx.doi.org/10.1016/j.ecoenv.2019.109612DOI Listing
November 2019

The Antiviral and Antitumor Effects of Defective Interfering Particles/Genomes and Their Mechanisms.

Front Microbiol 2019 9;10:1852. Epub 2019 Aug 9.

Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, China.

Defective interfering particles (DIPs), derived naturally from viral particles, are not able to replicate on their own. Several studies indicate that DIPs exert antiviral effects via multiple mechanisms. DIPs are able to activate immune responses and suppress virus replication cycles, such as competing for viral replication products, impeding the packaging, release and invasion of viruses. Other studies show that DIPs can be used as a vaccine against viral infection. Moreover, DIPs/DI genomes display antitumor effects by inducing tumor cell apoptosis and promoting dendritic cell maturation. With genetic modified techniques, it is possible to improve its safety against both viruses and tumors. In this review, a comprehensive discussion on the effects exerted by DIPs is provided. We further highlight the clinical significance of DIPs and propose that DIPs can open up a new platform for antiviral and antitumor therapies.
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http://dx.doi.org/10.3389/fmicb.2019.01852DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6696905PMC
August 2019

Research on Non-Contact Monitoring System for Human Physiological Signal and Body Movement.

Biosensors (Basel) 2019 Apr 19;9(2). Epub 2019 Apr 19.

School of Sino-Dutch Biomedical and Information Engineering, Northeastern University, Shenyang 110819, China.

With the rapid increase in the development of miniaturized sensors and embedded devices for vital signs monitoring, personal physiological signal monitoring devices are becoming popular. However, physiological monitoring devices which are worn on the body normally affect the daily activities of people. This problem can be avoided by using a non-contact measuring device like the Doppler radar system, which is more convenient, is private compared to video monitoring, infrared monitoring and other non-contact methods. Additionally real-time physiological monitoring with the Doppler radar system can also obtain signal changes caused by motion changes. As a result, the Doppler radar system not only obtains the information of respiratory and cardiac signals, but also obtains information about body movement. The relevant RF technology could eliminate some interference from body motion with a small amplitude. However, the motion recognition method can also be used to classify related body motion signals. In this paper, a vital sign and body movement monitoring system worked at 2.4 GHz was proposed. It can measure various physiological signs of the human body in a non-contact manner. The accuracy of the non-contact physiological signal monitoring system was analyzed. First, the working distance of the system was tested. Then, the algorithm of mining collective motion signal was classified, and the accuracy was 88%, which could be further improved in the system. In addition, the mean absolute error values of heart rate and respiratory rate were 0.8 beats/min and 3.5 beats/min, respectively, and the reliability of the system was verified by comparing the respiratory waveforms with the contact equipment at different distances.
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http://dx.doi.org/10.3390/bios9020058DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6627890PMC
April 2019

Reference genome sequences of two cultivated allotetraploid cottons, Gossypium hirsutum and Gossypium barbadense.

Nat Genet 2019 02 3;51(2):224-229. Epub 2018 Dec 3.

Biomarker Technologies Corporation, Beijing, China.

Allotetraploid cotton species (Gossypium hirsutum and Gossypium barbadense) have long been cultivated worldwide for natural renewable textile fibers. The draft genome sequences of both species are available but they are highly fragmented and incomplete. Here we report reference-grade genome assemblies and annotations for G. hirsutum accession Texas Marker-1 (TM-1) and G. barbadense accession 3-79 by integrating single-molecule real-time sequencing, BioNano optical mapping and high-throughput chromosome conformation capture techniques. Compared with previous assembled draft genomes, these genome sequences show considerable improvements in contiguity and completeness for regions with high content of repeats such as centromeres. Comparative genomics analyses identify extensive structural variations that probably occurred after polyploidization, highlighted by large paracentric/pericentric inversions in 14 chromosomes. We constructed an introgression line population to introduce favorable chromosome segments from G. barbadense to G. hirsutum, allowing us to identify 13 quantitative trait loci associated with superior fiber quality. These resources will accelerate evolutionary and functional genomic studies in cotton and inform future breeding programs for fiber improvement.
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http://dx.doi.org/10.1038/s41588-018-0282-xDOI Listing
February 2019

A Highly Sensitive Resistive Pressure Sensor Based on a Carbon Nanotube-Liquid Crystal-PDMS Composite.

Nanomaterials (Basel) 2018 Jun 8;8(6). Epub 2018 Jun 8.

National Engineering Lab for TFT-LCD Materials and Technologies, Department of Electronic Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.

Resistive pressure sensors generally employ microstructures such as pores and pyramids in the active layer or on the electrodes to reduce the Young’s modulus and improve the sensitivity. However, such pressure sensors always exhibit complex fabrication process and have difficulties in controlling the uniformity of microstructures. In this paper, we demonstrated a highly sensitive resistive pressure sensor based on a composite comprising of low-polarity liquid crystal (LPLC), multi-walled carbon nanotube (MWCNT), and polydimethylsiloxane (PDMS) elastomer. The LPLC in the PDMS forms a polymer-dispersed liquid crystal (PDLC) structure which can not only reduce the Young’s modulus but also contribute to the construction of conductive paths in the active layer. By optimizing the concentration of LC in PDMS elastomer, the resistive pressure sensor shows a high sensitivity of 5.35 kPa, fast response (<150 ms), and great durability. Fabrication process is also facile and the uniformity of the microstructures can be readily controlled. The pressure sensor offers great potential for applications in emerging wearable devices and electronic skins.
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http://dx.doi.org/10.3390/nano8060413DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6027416PMC
June 2018

[Successful perform of prolonged cardiopulmonary and cerebral resuscitation on repeated cardiac arrest: one case report].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2017 11;29(11):1032-1034

Emergency Center, Shihezi People's Hospital, Shihezi 832000, Xinjiang, China. Corresponding author: He Guanghui, Email:

Cardiac arrest (CA) is the most serious clinical emergency situation and cardiopulmonary-cerebral resuscitation (CPCR) performed on site with high quality is the optional therapy for its management. It has been reported that prolonging the resuscitation time after 30-minute failed conventional cardiopulmonary resuscitation (CPR) could improve the in-hospital survival rate of CA patients, and how to improve the out-hospital survival rate and survival quality of these patients is a research hot focus at present. A male patient admitted to Emergency Center of Shihezi People's Hospital reported in Xinjiang in this study had two CAs. In 2002, he experienced Adams-Strokes syndrome due to acute myocardial infarction (AMI) and survived after 35-minute of successful CPR. The criminal vessel was judged to re-canalize clinically 2 hours after thrombolytic therapy with urokinase, and he was cured and discharged from hospital 25 days later. In 2016, the second CA insult him and after the 185-minute CPR, he survived but experienced the post-CA syndrome. As long as 7-day continuous mild hypothermia was performed, the temperature of displacement fluids in continuous blood purification (CBP) was adjusted to 35 centigrade to achieve the goal of brain protection management requirements. He was cured and discharged from hospital 75 days later. During the 9-month follow-up, he did well in activities of daily living and could engage in routine housework. This paper introduces the treatment process of the patient in detail, and provides experience for clinical treatment.
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http://dx.doi.org/10.3760/cma.j.issn.2095-4352.2017.11.015DOI Listing
November 2017

Real-world cost-effectiveness of infliximab for moderate-to-severe rheumatoid arthritis in a medium-sized city of China.

J Comp Eff Res 2017 May 20;6(3):205-218. Epub 2016 Dec 20.

Health Economics and Outcomes Research, Normin Health Changsha Representative Office, Changsha, China.

Aim: To assess the cost-effectiveness of infliximab-containing therapy (ICT) for moderate-to-severe rheumatoid arthritis (RA) in a medium-sized Chinese city.

Methods: A Chinese prospective cohort study comparing ICT (25 patients) versus conventional disease-modified antirheumatic drugs (24 patients) for RA was used to assess the cost-effectiveness of ICT.

Results: The cohort study observed significantly reduced disease activity score of 28 joints (coefficient -2.718, p < 0.001), improved EQ-5D (coefficient 0.453, p < 0.001) and increased medical costs (coefficient 1.289, p < 0.001) associated with ICT. The incremental cost-effectiveness ratio per gained quality-adjusted life year for ICT versus disease-modified antirheumatic drugs was 1.897-times of the local gross domestic product per capita.

Conclusion: Infliximab was a favorable cost-effective alternative option for moderate-to-severe RA in a medium-sized city of China.
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http://dx.doi.org/10.2217/cer-2016-0086DOI Listing
May 2017

Combining MPDL3280A with adoptive cell immunotherapy exerts better antitumor effects against cervical cancer.

Bioengineered 2017 Jul 18;8(4):367-373. Epub 2016 Oct 18.

e Department of Urology , the Third Affiliated Hospital of Sun Yat-sen University , Guangzhou , P.R. China.

As the second most common gynecologic malignant tumors with a high mortality rate, cervical cancer jeopardizes women's life worldwide. The low cure rate in cervical cancer patients is mainly attributed to the lack of effective therapies. One feasible novel strategy is to develop immune-based approaches such as adoptive cell immunotherapy of DCCIKs which represents a promising nontoxic antineoplastic immunotherapy preferred in clinic practice. However, the therapeutic effect is not as efficient as anticipated. Possible explanations are tumors exploit immunoregulatory check-points such as programmed death 1(PD1)/PDL1 which provides tumor cells an escape strategy of circumventing immunologic rejection from immune surveillance by hampering activated tumor-specific T cell activities and rendering them functionally exhausted. With reduced transformation activity and enhanced antigenicity, a modified HPV16 E7 (HPV16mE7) was used to load DCs with silenced SOCS1 mediated by a recombinant adenovirus to improve the targetability and efficiency against cervical cancer. Combined with anti-PDL1 antibody MPDL3280A therapy, the co-cultured DCCIKs were transfused into murine models bearing tumor of HPV16 E6/E7 expressing CaSki cells for in vitro/in vivo antitumor activity assay. Although all of the animals succumbed to CaSki tumors even after adoptive DCCIKs transfer or MPDL3280A immunotherapy, the infusion of PDL1 blocking monoclonal antibody with activated T cells cured 40% of animals. These data support PDL1 blockade improves the efficacy of adoptive DCCIKs therapy, providing a new approach of immunotherapy against cervical cancer.
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http://dx.doi.org/10.1080/21655979.2016.1230573DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5553341PMC
July 2017

Treatment patterns, complications, and direct medical costs associated with ankylosing spondylitis in Chinese urban patients: a retrospective claims dataset analysis.

J Med Econ 2017 Jan 7;20(1):91-97. Epub 2016 Sep 7.

c Normin Health Changsha Representative Office , Changsha , PR China.

Aim: To describe treatment pattern, complications, and direct medical costs associated with ankylosing spondylitis (AS) in Chinese urban patients.

Methods: The 2013 China Health Insurance Research Association (CHIRA) urban insurance claims database was used to identify patients with AS. The identified patients were stratified by AS treatments for the comparisons of well established AS-related complications and direct medical costs. Conventional regression analyses adjusted the collected patient baseline characteristics to confirm the impact of treatments on complications and direct medical costs.

Results: Of the identified 1299 patients with AS, 18.0% received non-steroidal anti-inflammatory drugs (NSAID), 11.2% received immunosuppressant, 48.2% received NSAID plus immunosuppressant, 4.6% received biologic agents, and 17.9% received medications without indication for AS. Biologic group was associated with the lowest proportion of AS-related complications (8.3%) that was confirmed by multiple logistic regression analysis (odds ratio = 0.200, p = .017). The biologic group was also associated with highest direct medical costs (median: RMB = 14,539) that were confirmed by the multiple generalized linear model (coefficient = 1.644, p < .001).

Conclusions: Biologics were not commonly used for AS in Chinese patients likely due to their high cost. Future studies are needed to confirm the potential long-term clinical benefits associated biologic treatment for AS.
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http://dx.doi.org/10.1080/13696998.2016.1227829DOI Listing
January 2017

Cost-effectiveness of bortezomib for multiple myeloma: a systematic review.

Clinicoecon Outcomes Res 2016 3;8:137-51. Epub 2016 May 3.

Normin Health Changsha Representative Office, Changsha, People's Republic of China.

Objectives: To review published cost-effectiveness analyses (CEA) assessing bortezomib (BTZ) for multiple myeloma (MM) and explore possible bias affecting the cost-effectiveness of BTZ.

Methods: Literature was searched for published CEAs assessing BTZ or BTZ-containing regimens for MM from 2003 to 2015. The reported incremental cost-effectiveness ratios (ICER) were adjusted by 2014 country-specific gross domestic product per capita (GDPPC) to compare the cost-effectiveness threshold of the World Health Organization (3 GDPPC per gained quality-adjusted life year [QALY]).

Results: A total of 17 published CEAs were included in this review. When compared to non-BTZ treatments, BTZ-containing regimens were cost-effective for induction treatment prior to stem cell transplantation (SCT) in Canada, Poland, and Germany (ICER per QALY: 0.9299-2.254 GDPPC). BTZ/melphalan/prednisolone (VMP) was cost-effective for previously untreated and SCT-ineligible MM patients when compared to melphalan plus prednisolone (MP), melphalan/prednisone/lenalidomide with lenalidomide maintenance, and cyclophosphamide/thalidomide/dexamethasone (CTD) (ICER per QALY: dominant to 2.374 GDPPC) in Canada, UK, and USA. BTZ was cost-effective for relapsed/refractory MM when compared to best supportive care (ICER per life year: 0.9317-1.8210 GDPPC) in the UK and the USA, thalidomide in USA (0.5178 GDPPC/LY), and dexamethasone (DEX) in four Nordic countries (€54,451-€81,560/QALY). However, the cost-effectiveness for VMP versus MP plus thalidomide (MPT) and continuous lenalidomide (LEN) plus low-dose DEX (RD) for previously untreated and SCT-ineligible MM patients and BTZ versus LEN/DEX for relapsed/refractory MM patients could be unreliable because of the bias associated with model design and the indirect comparisons of treatment effects.

Conclusion: Published CEAs suggested that BTZ or BTZ-containing regimens were cost-effective when compared to most non-BTZ treatments for MM. However, the conflicting cost-effectiveness for VMP versus MPT for previously untreated and SCT-ineligible MM and BTZ versus LEN/DEX for relapsed/refractory MM needs more robust evidence for further clarification.
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http://dx.doi.org/10.2147/CEOR.S104195DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4861606PMC
May 2016

Real-world hospital costs for nonchemotherapy drugs and nondrug care associated with platinum-based doublets in the first-line setting for advanced nonsquamous non-small-cell lung cancer in Chinese patients: a retrospective cohort study.

Clinicoecon Outcomes Res 2016 26;8:97-111. Epub 2016 Apr 26.

Normin Health, Toronto, ON, Canada.

Objective: The objective of this study was to compare hospital costs per treatment cycle (HCTC) for nonchemotherapy drugs and nondrug care associated with platinum-based doublets in the first-line setting for advanced nonsquamous non-small-cell lung cancer (AdvNS-NSCLC) in Chinese patients.

Methods: Patients receiving platinum-based doublets in the first-line setting for AdvNS-NSCLC from 2010 to 2012 in two Chinese tertiary hospitals were identified to create the retrospective study cohort. Propensity score methods were used to create matched treatment groups for head-to-head comparisons on HCTC between pemetrexed-platinum and other platinum-based doublets. Multiple linear regression analyses were performed to rank studied platinum-based doublets for their associations with the log10 scale of HCTC for nonchemotherapy drugs and nondrug care.

Results: Propensity score methods created matched treatment groups for pemetrexed-platinum versus docetaxel-platinum (61 pairs), paclitaxel-platinum (39 pairs), gemcitabine-platinum (93 pairs), and vinorelbine-platinum (73 pairs), respectively. Even though the log10 scale of HCTC for nonchemotherapy drugs and nondrug care associated with pemetrexed-platinum was ranked lowest in all patients (coefficient -0.174, P=0.015), which included patients experiencing any hematological adverse events (coefficient -0.199, P=0.013), neutropenia (coefficient -0.426, P=0.021), or leukopenia (coefficient -0.406, P=0.001), pemetrexed-platinum had the highest total HCTC (median difference from RMB 1,692 to RMB 7,400, P<0.001) among platinum-based doublets because of its higher drug acquisition costs (median difference from RMB 4,636 to RMB 7,332, P<0.001).

Conclusion: Among Chinese patients receiving platinum-based doublets in the first-line setting for AdvNS-NSCLC, the higher acquisition costs for nonplatinum cytotoxic drugs associated with pemetrexed-platinum could be partially offset by its significantly lower hospital costs for nonchemotherapy drugs and nondrug care.
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http://dx.doi.org/10.2147/CEOR.S98548DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4853010PMC
May 2016

Posthospital Discharge Medical Care Costs and Family Burden Associated with Osteoporotic Fracture Patients in China from 2011 to 2013.

J Osteoporos 2015 28;2015:258089. Epub 2015 Jun 28.

Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430074, China.

Objectives. This study collected and evaluated data on the costs of outpatient medical care and family burden associated with osteoporosis-related fracture rehabilitation following hospital discharge in China. Materials and Methods. Data were collected using a patient questionnaire from osteoporosis-related fracture patients (N = 123) who aged 50 years and older who were discharged between January 2011 and January 2013 from 3 large hospitals in China. The survey captured posthospital discharge direct medical costs, indirect medical costs, lost work time for caregivers, and patient ambulatory status. Results. Hip fracture was the most frequent fracture site (62.6%), followed by vertebral fracture (34.2%). The mean direct medical care costs per patient totaled 3,910¥, while mean indirect medical costs totaled 743¥. Lost work time for unpaid family caregivers was 16.4 days, resulting in an average lost income of 3,233¥. The average posthospital direct medical cost, indirect medical cost, and caregiver lost income associated with a fracture patient totaled 7,886¥. Patients' ambulatory status was negatively impacted following fracture. Conclusions. Significant time and cost of care are placed on patients and caregivers during rehabilitation after discharge for osteoporotic fracture. It is important to evaluate the role and responsibility for creating the growing and inequitable burden placed on patients and caregivers following osteoporotic fracture.
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http://dx.doi.org/10.1155/2015/258089DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4499404PMC
July 2015

Inpatient cost of treating osteoporotic fractures in mainland China: a descriptive analysis.

Clinicoecon Outcomes Res 2015 13;7:205-12. Epub 2015 Apr 13.

Global Patient Outcomes and Real World Evidence, Bio-Medicines, Eli Lilly and Company, Indianapolis, IN, USA.

Purpose: The objective of this study was to provide new estimates on the per-admission inpatient hospital cost and per-admission length of stay (LOS) for osteoporosis-related fractures in mainland China.

Materials And Methods: Data for inpatient hospitalization associated with at least one osteoporosis-related fracture were obtained from the nationwide China Health Insurance Research Association and were analyzed post hoc. Patients' data were included if the patients were ≥50 years old and diagnosed with osteoporosis and pathologic fracture, or osteoporosis therapy and fragility fracture by an International Statistical Classification of Diseases and Related Health Problems, 10th Revision (ICD-10) code designation, between 2008 and 2010.

Results: The analysis included 830 patients (female: 77.3%; mean age: 73.4±9.8 years). The medians of the per-admission LOS and inpatient costs were 19 days and ¥18,587, respectively. Longer LOS and higher costs per admission were associated with older patients (≥70 years) compared to younger patients (<70 years). Hip fracture had the longest median LOS (22 days) and highest median cost (¥32,594) among all fracture sites. The per-hospitalization episode and per-day costs of osteoporotic fracture increased rapidly (60% and 89%, respectively) between 2008 and 2010.

Conclusion: The analysis showed that hospitalization cost increases were associated with increasing per-day hospitalization costs. The proportion of the costs reimbursed by health insurances increased, while the mean absolute patient copayment amounts decreased. The incidence and prevalence of osteoporosis and osteoporosis-related fractures may rise rapidly due to the projected growth of the aged population in mainland China. Therefore, the combination of greater anticipated total fractures and rising hospital costs may lead to a tremendously increased economic burden in the future.
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http://dx.doi.org/10.2147/CEOR.S77175DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4403816PMC
April 2015