Publications by authors named "Yi-Ming Wang"

150 Publications

Career choice and influential factors among medical students majoring in psychiatry in China.

BMC Med Educ 2021 Mar 25;21(1):183. Epub 2021 Mar 25.

Peking University Sixth Hospital, Peking University Institute of Mental Health, NHC Key Laboratory of Mental Health (Peking University), National Clinical Research Center for Mental Disorders (Peking University Sixth Hospital), 51 Huayuan Bei Road, Beijing, 100191, China.

Background: The undergraduate program of psychiatry has been widely established in recent years to improve the education and recruitment of psychiatrists in China. We aim to investigate the career choice of medical students majoring in psychiatry in China and the influential factors.

Method: This multicenter study was conducted in 26 medical schools in China from May to October of 2019. Participants included 4610 medical students majoring in psychiatry and 3857 medical students majoring in clinical medicine. Multivariable logistic regression was used to investigate the influential factors of students' choices of psychiatry at matriculation and as a career.

Results: 44.08% of psychiatry majored students gave psychiatry as a first choice at matriculation, and 56.67% of them would choose psychiatry as a career, which was in sharp contrast to the proportion of clinical medicine majored students who would choose psychiatry as a career (0.69%). Personal interest (59.61%), suggestions from family members (27.96%), and experiencing mental problems (23.19%) were main reasons for choosing psychiatry major at matriculation. Personal interest (odds ratio [OR] = 2.12, 95% confidence interval [CI] = 1.87-2.40), experiencing a psychiatry clerkship (OR = 1.99, 95% CI = 1.28-3.08), being female (OR = 1.50, 95% CI = 1.30-1.68), experiencing mental problems (OR = 1.33, 95% CI = 1.28-1.56), and suggestions from family members (OR = 1.25, 95% CI = 1.08-1.46) correlated positively with students' choice of psychiatry as career. Students who lacked psychiatry knowledge (OR = 0.49, 95% CI = 0.29-0.85) or chose psychiatry because of lower admission scores (OR = 0.80, 95% CI = 0.63-0.97) were less likely to choose psychiatry as a career.

Conclusion: More than half of psychiatry majored medical school students planned to choose psychiatry as their career, whereas very few students in the clinic medicine major would make this choice. Increasing students' interest in psychiatry, strengthening psychiatry clerkships, and popularizing psychiatric knowledge are modifiable factors to increase the psychiatry career intention. The extent to which medical students' attitudes toward psychiatry can be changed through medical school education and greater exposure to psychiatry will need further investigation.
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http://dx.doi.org/10.1186/s12909-021-02622-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7992123PMC
March 2021

A deep-learning-based prognostic nomogram integrating microscopic digital pathology and macroscopic magnetic resonance images in nasopharyngeal carcinoma: a multi-cohort study.

Ther Adv Med Oncol 2020 14;12:1758835920971416. Epub 2020 Dec 14.

Guangdong Provincial Key Laboratory of Biomedical Imaging, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai, Guangdong Province 519000, P. R. China.

Background: To explore the prognostic value of radiomics-based and digital pathology-based imaging biomarkers from macroscopic magnetic resonance imaging (MRI) and microscopic whole-slide images for patients with nasopharyngeal carcinoma (NPC).

Methods: We recruited 220 NPC patients and divided them into training ( = 132), internal test ( = 44), and external test ( = 44) cohorts. The primary endpoint was failure-free survival (FFS). Radiomic features were extracted from pretreatment MRI and selected and integrated into a radiomic signature. The histopathological signature was extracted from whole-slide images of biopsy specimens using an end-to-end deep-learning method. Incorporating two signatures and independent clinical factors, a multi-scale nomogram was constructed. We also tested the correlation between the key imaging features and genetic alternations in an independent cohort of 16 patients (biological test cohort).

Results: Both radiomic and histopathologic signatures presented significant associations with treatment failure in the three cohorts (C-index: 0.689-0.779, all  < 0.050). The multi-scale nomogram showed a consistent significant improvement for predicting treatment failure compared with the clinical model in the training (C-index: 0.817 0.730,  < 0.050), internal test (C-index: 0.828 0.602,  < 0.050) and external test (C-index: 0.834 0.679,  < 0.050) cohorts. Furthermore, patients were stratified successfully into two groups with distinguishable prognosis (log-rank  < 0.0010) using our nomogram. We also found that two texture features were related to the genetic alternations of chromatin remodeling pathways in another independent cohort.

Conclusion: The multi-scale imaging features showed a complementary value in prognostic prediction and may improve individualized treatment in NPC.
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http://dx.doi.org/10.1177/1758835920971416DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7739087PMC
December 2020

A logistic model and predictions for the spread of the COVID-19 pandemic.

Chaos 2020 Dec;30(12):123135

Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA.

The rapid spread of COVID-19 worldwide presents a great challenge to epidemic modelers. Model outcomes vary widely depending on the characteristics of a pathogen and the models. Here, we present a logistic model for the epidemic spread and divide the spread of the novel coronavirus into two phases: the first phase is a natural exponential growth phase that occurs in the absence of intervention and the second phase is a regulated growth phase that is affected by enforcing social distancing and isolation. We apply the model to a number of pandemic centers. Our results are in good agreement with the data to date and show that social distancing significantly reduces the epidemic spread and flattens the curve. Predictions on the spreading trajectory including the total infections and peak time of new infections for a community of any size are made weeks ahead, providing the vital information and lead time needed to prepare for and mitigate the epidemic. The methodology presented here has immediate and far-reaching applications for ongoing outbreaks or similar future outbreaks of other emergent infectious diseases.
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http://dx.doi.org/10.1063/5.0028236DOI Listing
December 2020

Developing and validating an integrated gross tumor volume (GTV)-TNM stratification system for supplementing unresectable locally advanced non-small cell lung cancer treated with concurrent chemoradiotherapy.

Radiat Oncol 2020 Nov 10;15(1):260. Epub 2020 Nov 10.

Department of Radiation Oncology, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-Sen University Cancer Center, 651 Dongfeng Road East, Guangzhou, Guangdong, 510060, People's Republic of China.

Purpose: The gross tumor volume (GTV) could be an independent prognostic factor for unresectable locally advanced non-small cell lung cancer (LANSCLC). We aimed to develop and validate a novel integrated GTV-TNM stratification system to supplement LANSCLC sub-staging in patients treated with concurrent chemoradiotherapy (CCRT).

Methods: We performed a retrospective review of 340 patients with unresectable LANSCLC receiving definitive CCRT. All included patients were divided into two randomized cohorts. Then the Kaplan-Meier method and Cox regression were calculated to access the prognostic value of the integrated GTV-TNM stratification system, which was further validated by the area under the receiver operating characteristic curve (AUC) score and F1-score.

Results: The optimal outcome-based GTV cut-off values (70 and 180 cm) of the modeling cohort were used to determine each patient's integrated GTV-TNM stratum in the whole cohort. Our results indicated that a lower integrated GTV-TNM stratum could had better overall survival and progression-free survival (all P < 0.001), which was recognized as an independent prognostic factor. Also, its prognostic value was robust in both the modeling and validation cohorts. Furthermore, the prognostic validity of the integrated GTV-TNM stratification system was validated by significantly improved AUC score (0.636 vs. 0.570, P = 0.027) and F1-score (0.655 vs. 0.615, P < 0.001), compared with TNM stage.

Conclusions: We proposed a novel integrated GTV-TNM stratification system to supplement unresectable LANSCLC sub-staging due to its prognostic value independent of TNM stage and other clinical characteristics, suggesting that it could be considered in individual treatment decision-making process.
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http://dx.doi.org/10.1186/s13014-020-01704-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7653712PMC
November 2020

Metal-Organic Framework-Encapsulated CoCu Nanoparticles for the Selective Transfer Hydrogenation of Nitrobenzaldehydes: Engineering Active Armor by the Half-Way Injection Method.

Chemistry 2021 Jan 27;27(3):1080-1087. Epub 2020 Dec 27.

Department of Chemistry, College of Sciences, Northeastern University, Shenyang, 110819, P. R. China.

A novel armor-type composite of metal-organic framework (MOF)-encapsulated CoCu nanoparticles with a Fe O core (Fe O @SiO -NH -CoCu@UiO-66) has been designed and synthesized by the half-way injection method, which successfully serves as an efficient and recyclable catalyst for the selective transfer hydrogenation. In this half-way injection approach, the pre-synthetic Fe O @SiO -NH -CoCu was injected into the UiO-66 precursor solution halfway through the MOF budding period. The formed MOF armor could play a role of providing significant additional catalytic sites besides CoCu nanoparticles, protecting CoCu nanoparticles, and improving the catalyst stability, thus facilitating the selective transfer hydrogenation of nitrobenzaldehydes into corresponding nitrobenzyl alcohols in high selectivity (99 %) and conversion (99 %) rather than nitro group reduction products. Notably, this method achieves the precise assembly of a MOF-encapsulated composite, and the ingenious combination of MOF and nanoparticles exhibits excellent catalytic performance in the selective hydrogen transfer reaction, implementing a "1+1>2" strategy in catalysis.
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http://dx.doi.org/10.1002/chem.202003857DOI Listing
January 2021

A Study of Defect Detection Techniques for Metallographic Images.

Sensors (Basel) 2020 Sep 29;20(19). Epub 2020 Sep 29.

Department of Mechatoronics Engineering, National Changhua University of Education, Changhua City 50007, Taiwan.

Metallography is the study of the structure of metals and alloys. Metallographic analysis can be regarded as a detection tool to assist in identifying a metal or alloy, to evaluate whether an alloy is processed correctly, to inspect multiple phases within a material, to locate and characterize imperfections such as voids or impurities, or to find the damaged areas of metallographic images. However, the defect detection of metallography is evaluated by human experts, and its automatic identification is still a challenge in almost every real solution. Deep learning has been applied to different problems in computer vision since the proposal of AlexNet in 2012. In this study, we propose a novel convolutional neural network architecture for metallographic analysis based on a modified residual neural network (ResNet). Multi-scale ResNet (M-ResNet), the modified method, improves efficiency by utilizing multi-scale operations for the accurate detection of objects of various sizes, especially small objects. The experimental results show that the proposed method yields an accuracy of 85.7% (mAP) in recognition performance, which is higher than existing methods. As a consequence, we propose a novel system for automatic defect detection as an application for metallographic analysis.
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http://dx.doi.org/10.3390/s20195593DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7583772PMC
September 2020

Automatic assessment of bone age in Taiwanese children: A comparison of the Greulich and Pyle method and the Tanner and Whitehouse 3 method.

Kaohsiung J Med Sci 2020 Nov 3;36(11):937-943. Epub 2020 Aug 3.

Department of Medical Imaging, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.

Proper bone age assessment is crucial for the clinical diagnosis and evaluation of treatment responses. We investigated the applicability of Greulich and Pyle (GP), and Tanner and Whitehouse 3 (TW3) methods for children in modern Taiwan, using computer-aided diagnosis. Hand and wrist radiographs were obtained from 611 children (3-17 years) who came to our emergency department due to trauma. Ages 0 to 2 years old were excluded because of a limited number of cases. Skeletal maturation was assessed using the BoneXpert (version 2.5.4.1 automated software), which determines GP and TW3 bone age. The two scoring systems were evaluated for comparing the chronological ages in each subgroup. In boys, mean GP bone age vs mean chronological ages were delayed for ages 3 to 11 and advanced for age 12 to 17. In girls, mean GP bone age vs mean chronological ages was delayed for ages 4 to 8 and 17, and advanced for ages 3 and 9 to 17. In boys, the mean TW3 bone ages vs mean chronological ages were delayed for ages 5 to 10 except age 8, and advanced for ages 3 to 4, 8, and 11 to 15. In girls, the mean TW3 bone ages vs mean chronological ages were delayed for ages 4 to 12, and advanced for ages 3 and 13 to 14. By using the BoneXpert automatic software, we established bone age reference standards for children in Taiwan. Clinical application of GP and TW3 scoring methods can be adjusted according to our results to better assess bone age.
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http://dx.doi.org/10.1002/kjm2.12268DOI Listing
November 2020

Stereodivergent Alkyne Hydrofluorination Using Protic Tetrafluoroborates as Tunable Reagents.

Angew Chem Int Ed Engl 2020 09 14;59(38):16651-16660. Epub 2020 Jul 14.

Department of Chemistry, University of Pittsburgh, Pittsburgh, PA, 15260, USA.

The discovery of safe, general, and practical procedures to prepare vinyl fluorides from readily available precursors remains a synthetic challenge. The metal-free hydrofluorination of alkynes constitutes an attractive though elusive strategy for their preparation. Introduced here is an inexpensive and easily handled reagent that enables the development of simple and scalable protocols for the regioselective hydrofluorination of alkynes to access both the E and Z isomers of vinyl fluorides. These reaction conditions were suitable for a diverse collection of alkynes, including several highly functionalized pharmaceutical derivatives. Computational and experimental mechanistic studies support C-F bond formation through vinyl cation intermediates, with the E- and Z-hydrofluorination products forming under kinetic and thermodynamic control, respectively.
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http://dx.doi.org/10.1002/anie.202006278DOI Listing
September 2020

Stereodivergent Alkyne Hydrofluorination Using Protic Tetrafluoroborates as Tunable Reagents.

Angew Chem Int Ed Engl 2020 09 14;59(38):16651-16660. Epub 2020 Jul 14.

Department of Chemistry, University of Pittsburgh, Pittsburgh, PA, 15260, USA.

The discovery of safe, general, and practical procedures to prepare vinyl fluorides from readily available precursors remains a synthetic challenge. The metal-free hydrofluorination of alkynes constitutes an attractive though elusive strategy for their preparation. Introduced here is an inexpensive and easily handled reagent that enables the development of simple and scalable protocols for the regioselective hydrofluorination of alkynes to access both the E and Z isomers of vinyl fluorides. These reaction conditions were suitable for a diverse collection of alkynes, including several highly functionalized pharmaceutical derivatives. Computational and experimental mechanistic studies support C-F bond formation through vinyl cation intermediates, with the E- and Z-hydrofluorination products forming under kinetic and thermodynamic control, respectively.
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http://dx.doi.org/10.1002/anie.202006278DOI Listing
September 2020

Physiological and transcriptomic analysis provide novel insight into cobalt stress responses in willow.

Sci Rep 2020 02 11;10(1):2308. Epub 2020 Feb 11.

College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China.

Cobalt (Co) is an essential component of several enzymes and coenzymes in living organisms. Excess Co is highly toxic to plants. The knowledge of molecular response mechanisms to cobalt stress in plants is still limited, especially in woody plants. The responses of weeping willow (Salix babylonica) seedlings to Co stress were studied using morphological and physiochemical measurements and RNA-seq analysis. The physiological and biochemical indexes such as growth rate, the content of chlorophyll and soluble sugar, photosynthesis and peroxidase activity were all changed in willow seedlings under Co stress. The metal ion concentrations in willow including Cu, Zn and Mg were disturbed due to excess Co. Of 2002 differentially expressed genes (DEGs), 1165 were root-specific DEGs and 837 were stem and leaf-specific DEGs. Further analysis of DEGs showed there were multiple complex cascades in the response network at the transcriptome level under Co stress. Detailed elucidation of responses to oxidative stress, phytohormone signaling-related genes and transcription factors (TFs), and detoxification of excess cellular Co ion indicated the various defense mechanisms in plants respond to cobalt stress. Our findings provide new and comprehensive insights into the plant tolerance to excess Co stress.
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http://dx.doi.org/10.1038/s41598-020-59177-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7012891PMC
February 2020

Improving the accuracy and efficacy of diagnosing polycystic ovary syndrome by integrating metabolomics with clinical characteristics: study protocol for a randomized controlled trial.

Trials 2020 Feb 11;21(1):169. Epub 2020 Feb 11.

Department of Endocrinology, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Nanjing Medical University, Wuxi, 214023, China.

Background: Polycystic ovary syndrome (PCOS) is a complex endocrine syndrome with poorly understood mechanisms. To provide patients with PCOS with individualized therapy, it is critical to precisely diagnose the phenotypes of the disease. However, the criteria for diagnosing the different phenotypes are mostly based on symptoms, physical examination and laboratory results. This study aims to compare the accuracy and efficacy of diagnosing PCOS by integrating metabolomic markers with common clinical characteristics.

Methods: This is a prospective, multicenter, analyst-blinded, randomized controlled trial. Participants will be grouped into (1) people without PCOS (healthy control group), (2) patients diagnosed with PCOS based on clinical indices (experimental group 1), and (3) patients diagnosed with PCOS based on metabolomic indices (experimental group 2). A total of 276 participants, including 60 healthy people and 216 patients with PCOS, will be recruited. The 216 patients with PCOS will be randomly assigned to the two experimental groups in a 1:1 ratio, and each group will receive a different 6-month treatment. The primary outcome for the experimental groups will be the effect of PCOS treatment.

Discussion: The results of this trial should help to determine whether using metabolomic indices is more accurate and effective than using clinical characteristics in diagnosing the phenotypes of PCOS. The results could provide a solid foundation for the accurate diagnosis of different PCOS subgroups and for future research on individualized PCOS therapy.

Trial Registration: Chinese Clinical Trial Registry, ID: ChiCTR-INR-1800016346. Registered 26 May 2018.
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http://dx.doi.org/10.1186/s13063-020-4060-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7014640PMC
February 2020

High Cell Selectivity and Bactericidal Mechanism of Symmetric Peptides Centered on d-Pro-Gly Pairs.

Int J Mol Sci 2020 Feb 8;21(3). Epub 2020 Feb 8.

College of Animal Science and Technology, Jilin Agricultural University, Xincheng Street No.#2888, Changchun 130118, China.

Antimicrobial peptides (AMPs) have a unique action mechanism that can help to solve global problems in antibiotic resistance. However, their low therapeutic index and poor stability seriously hamper their development as therapeutic agents. In order to overcome these problems, we designed peptides based on the sequence template XXRXXRRzzRRXXRXX-NH, where X represents a hydrophobic amino acid like Phe (F), Ile (I), and Leu (L), while zz represents Gly-Gly (GG) or d-Pro-Gly (pG). Showing effective antimicrobial activity against Gram-negative bacteria and low toxicity, designed peptides had a tendency to form an α-helical structure in membrane-mimetic environments. Among them, peptide LR (X: L, zz: pG) showed the highest geometric mean average treatment index (GM = 73.1), better salt, temperature and pH stability, and an additive effect with conventional antibiotics. Peptide LR played the role of anti-Gram-negative bacteria through destroying the cell membrane. In addition, peptide LR also exhibited an anti-inflammatory activity by effectively neutralizing endotoxin. Briefly, peptide LR has the potential to serve as a therapeutic agent to reduce antibiotic resistance owing to its high therapeutic index and great stability.
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http://dx.doi.org/10.3390/ijms21031140DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037546PMC
February 2020

α-C-H Functionalization of π-Bonds Using Iron Complexes: Catalytic Hydroxyalkylation of Alkynes and Alkenes.

J Am Chem Soc 2019 12 5;141(50):19594-19599. Epub 2019 Dec 5.

Department of Chemistry , University of Pittsburgh , Pittsburgh , Pennsylvania 15260 , United States.

The discovery of catalytic systems based on earth-abundant transition metals for the functionalization of C-H bonds enables streamlined and sustainable solutions to problems in synthetic organic chemistry. In this Communication, we disclose an iron-based catalytic system for the functionalization of propargylic and allylic C-H bonds. Inexpensive and readily available cyclopentadienyliron(II) dicarbonyl complexes were employed as catalysts for a novel deprotonative activation mode for C-H functionalization, an approach that allows for the direct union of unsaturated building blocks with aryl aldehydes and other carbonyl electrophiles to deliver a range of unsaturated alcohol coupling products under operationally simple and functional group tolerant reaction conditions.
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http://dx.doi.org/10.1021/jacs.9b11716DOI Listing
December 2019

Functional analysis of hisJ in Aeromonas veronii reveals a key role in virulence.

Ann N Y Acad Sci 2020 04 30;1465(1):146-160. Epub 2019 Oct 30.

College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.

Aeromonas veronii is an important aquatic zoonotic pathogen in humans and animals. In recent years, extracellular proteins from bacteria have been found to be the major pathogenic factors for aquatic animals. The aim of this study was to systematically analyze the extracellular proteins of nine sources of A. veronii and the effects of hisJ on virulence. We screened only the common proteins from nine different sources of A. veronii by liquid chromatography-tandem mass spectrometry and identified the gene hisJ. We then constructed ΔhisJ (deleted) and C-hisJ (complemented) variants of A. veronii TH0426 to assess the biological function of hisJ. While the ΔhisJ strain did not show altered growth (P > 0.05), we observed that it had reduced colony formation and biofilm formation and reduced adhesion to and invasion of epithelioma papulosum cyprini cells by 2.0-, 1.9-, and 10.8-fold, respectively. Additionally, infection experiments on zebrafish and mouse infection experiments showed that the virulence of the ΔhisJ strain was decreased by 865-fold (P < 0.001) compared with the wild-type strain; virulence of the complemented C-hisJ strain was reduced only 2.8-fold. Furthermore, in the context of hisJ deletion, flagella of A. veronii TH0426 were easily detached and the expression of virulence genes was downregulated. A persistence test (of bacterial colonies in crucian carp) showed that the number of bacteria in the immune organs of the ΔhisJ-infected group was lower than that in the wild-type-infected group. Overall, these results show that hisJ affects flagellar shedding, virulence, biofilm formation, adhesion, and invasion of A. veronii TH0426, and that hisJ is closely associated with virulence and plays a crucial role in its pathogenicity of A. veronii TH0426.
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http://dx.doi.org/10.1111/nyas.14265DOI Listing
April 2020

Characterization of bacterial community changes and antibiotic resistance genes in lamb manure of different incidence.

Sci Rep 2019 07 12;9(1):10101. Epub 2019 Jul 12.

Department of Basic Veterinary Medicine, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.

Bacterial enteritis is the most important disease in lamb for breeding greatly affects the growth of animals. Changes in the community of intestinal flora can cause the disorder of the colonic environment induced diarrhea. This study aimed to investigate the relationship between the incidence of bacterial enteritis and the number of intestinal microbiome, then the prevalence of drug-resistant genes was detected. Fecal samples were collected at five fattening sheep farms with different incidence of bacterial enteritis, pathogenic bacteria were isolated and identified, drug sensitivity tests were performed. Then, changes in number and structure of intestinal flora were compared by 16S rDNA V3-V4 region high-throughput sequencing, and the ARGs were detected using high-throughput real-time PCR. Our results revealed that the microbial communities were positively correlated with the incidence of bacterial enteritis in different farms. Bacterial communities were higher in YJ (with highest incidence of diarrhea) than any other farms. However, the ARGs seemed not to be more affected by the incidence of bacterial enteritis, but one of the significant findings to emerge from this study is that MCR-1 and NDM are detected in manure. This study has provided an insight of the changes occurring in intestinal flora and AGRs in fattening sheep farms with diverse incidence of bacterial enteritis.
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http://dx.doi.org/10.1038/s41598-019-46604-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6625992PMC
July 2019

Antibacterial mode of fibrauretine and synergistic effect with kanamycin against multi-drug resistant Escherichia coli.

Biotechnol Lett 2019 Sep 15;41(8-9):1023-1031. Epub 2019 Jun 15.

College of Animal Science and Technology, Jilin Agricultural University, Xincheng Street No. 2888, Changchun, 130118, China.

Objective: The present study evaluated the antibacterial activity and mode of action of fibrauretine on Escherichia coli (E. coli) and Staphylococcus aureus, and synergistic effect with kanamycin against multi-drug resistant E. coli.

Results: The fibrauretine exhibited inhibitory effect on the growth of the tested bacteria with minimum inhibitory concentration (MIC) and minimum bactericidal concentration of 2.5-5 and 5-10 mg/ml, respectively. Morphological changes of cell microstructure were observed after adding fibrauretine at MIC. The mode of action was further confirmed by measuring release of 260-nm absorbing materials and extracellular potassium ions. Checkerboard dilution test suggested that fibrauretine exhibited synergistic activity when combined with kanamycin (FICI ranging from 0.5625 to 0.625).

Conclusions: Our results indicated that fibrauretine exerted synergistic effect with kanamycin and its antibacterial mode of action mainly attributed to disruption of cell membrane integrity.
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http://dx.doi.org/10.1007/s10529-019-02697-zDOI Listing
September 2019

Transcriptome Analysis Reveals AI-2 Relevant Genes of Multi-Drug Resistant in Response to Eugenol at Sub-MIC.

Front Microbiol 2019 28;10:1159. Epub 2019 May 28.

College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.

Eugenol, the major active essential oil component of clove, was reported to possess QS (quorum sensing) inhibitory activity. A previous study found that eugenol could bind to quorum sensing receptors of and down-regulate the expression of virulence genes at sub-MIC (minimum inhibitory concentration) without affecting the bacterial growth. However, the alterations of QS signal molecules at transcription levels was not well understood. To better understand interactions of in response to eugenol and explore molecular regulations, transcriptome sequencing was performed. A total of 5779 differentially expressed genes (DEGs) enriched in a variety of biological processes and pathways were identified. The transcriptional data was validated by qPCR and the results showed that the expression profiles of 4 major genes involved in autoinducers-2 (AI-2) synthesis, including , , and were consistent with transcriptome analysis except for , a transcriptional repressor gene of lsr operon, which may repress the expression of following genes responsible for AI-2 signal transmission . AI-2 synthesis assay also revealed that eugenol could inhibit AI-2 generation. The results of our study offer insights into the mechanisms of QS inhibitory activity and AI-2 alterations after eugenol treatment.
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http://dx.doi.org/10.3389/fmicb.2019.01159DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6547871PMC
May 2019

NRAMP1 promotes iron uptake at the late stage of iron deficiency in poplars.

Tree Physiol 2019 07;39(7):1235-1250

State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China.

Iron (Fe) is an essential micronutrient for plant survival and proliferation. Plants have evolved complex mechanisms to maintain Fe homeostasis in response to Fe deficiency. In this study, we evaluated the physiological, biochemical and transcriptomic differences between poplars grown under Fe-sufficient and Fe-deficient conditions to elucidate the mechanistic responses of poplars to Fe deficiency. Our results revealed that chlorophyll synthesis and photosynthesis were inhibited under Fe-deficient conditions. The inhibition of these pathways caused chlorosis and reduced shoot growth. Although both photosynthetic systems (PSI and PSII) were inhibited under Fe limitation, PSI was affected more severely and earlier than PSII. Fe deficiency also promoted root growth and increased the accumulation of divalent metal ions in roots. IRT1 and NRAMP1 are both Fe2+ transporters for iron uptake in Arabidopsis. In this study, however, only NRAMP1 was induced to promote Fe2+ uptake in roots at the late stage of Fe deficiency response. It indicated that NRAMP1, rather than the more well-known IRT1, might be a major Fe2+ transporter at the late stage of Fe-deficiency in poplars.
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http://dx.doi.org/10.1093/treephys/tpz055DOI Listing
July 2019

Vasitis: a rare diagnosis mimicking inguinal hernia: a case report.

BMC Urol 2019 Apr 29;19(1):27. Epub 2019 Apr 29.

Department of Radiology, Kaohsiung Medical University Hospital, Kaohsiung Medical University, No. 100, Ziyou 1st Rd., Sanmin District, Kaohsiung City, 80756, Taiwan, Republic of China.

Background: Vasitis or inflammation of the vas deferens is a rare condition, and few case reports with computed tomography images have been published since 1980.

Case Presentation: A 50-year-old man presented with severe right inguinal and lower abdominal pain. Initial diagnosis at the emergency department was incarcerated or strangulated inguinal hernia. The computed tomography scan revealed diffuse edematous changes of right spermatic cord and vas deferens with peripheral fat stranding. Correlating with his clinical symptoms, signs, and imaging findings, the diagnosis of vasitis was made. We report a case of acute vasitis about the cause, symptom, pathogen, differential diagnoses, image findings, and treatment.

Conclusion: Although very rare, vasitis should be listed as one of the differential diagnosis for inguinal mass lesions. Cross-sectional imaging may be necessary to confirm the diagnosis and exclude differentials such as an inguinal hernia. Recognition of the characteristic image findings can help to make the correct diagnosis and avoid unnecessary surgery.
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http://dx.doi.org/10.1186/s12894-019-0460-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6489350PMC
April 2019

Comparison of hyper- and hypofractionated radiation schemes with IMRT technique in small cell lung cancer: Clinical outcomes and the introduction of extended LQ and TCP models.

Radiother Oncol 2019 07 15;136:98-105. Epub 2019 Apr 15.

Department of Radiation Oncology, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China. Electronic address:

Purpose: To evaluate the outcomes of 45 Gy/15 fractions/once-daily and 45 Gy/30 fractions/twice-daily radiation schemes utilizing intensity-modulated radiation therapy (IMRT) in extensive stage small cell lung cancer (SCLC), and to build up a new radiobiological model for tumor control probability (TCP) considering multiple biological effects.

Methods: Fifty-eight consecutive patients diagnosed with extensive stage SCLC, treated with chemotherapy and chest irradiation, were retrospectively reviewed. Thirty-seven received hyperfractionated IMRT (Hyper-IMRT, 45 Gy/30 fractions/twice-daily) and 21 received hypofractionated IMRT (Hypo-IMRT, 45 Gy/15 fractions/once-daily). Local progression-free survival (LPFS) and overall survival (OS) were calculated and compared. An extended linear-quadratic (LQ) model, LQRG, incorporating cell repair, redistribution, reoxygenation, regrowth and Gompertzian tumor growth was created based on the clinical data. The TCP model was reformulated to predict LPFS. The classical LQ and TCP models were compared with the new models. Akaike information criterion (AIC) was used to assess the quality of the models.

Results: The 2-year LPFS (34.1% vs 27.9%, p = 0.44) and OS (76.9% vs 76.9%, p = 0.26) were similar between Hyper- and Hypo-IMRT patients. According to the LQRG model, the α/β calculated was 9.2 (95% confidence interval: 8.7-9.9) Gy after optimization. The average absolute and relative fitting errors for LPFS were 9.1% and 18.7% for Hyper-IMRT, and 8.8% and 16.2% for Hypo-IMRT of the new TCP model, compared with 29.1% and 62.3% for Hyper-IMRT, and 30.7% and 65.3% for Hypo-IMRT of the classical model.

Conclusions: Hypo- and Hyper-IMRT resulted in comparable local control in the chest irradiation of extensive stage SCLC. The LQRG model has better performance in predicting the TCP (or LPFS) of the two schemes.
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http://dx.doi.org/10.1016/j.radonc.2019.03.035DOI Listing
July 2019

Rhodium-Catalyzed Coupling-Cyclization of Alkenyldiazoacetates with o-Alkenyl Arylisocyanides: A General Route to Carbazoles.

Org Lett 2019 05 22;21(9):2973-2977. Epub 2019 Mar 22.

Jilin Province Key Laboratory of Organic Functional Molecular Design & Synthesis, Faculty of Chemistry , Northeast Normal University , Changchun 130024 , China.

A rhodium-catalyzed coupling-cyclization reaction of alkenyldiazoacetates with o-alkenyl arylisocyanides has been developed. In this reaction, the highly reactive alkenylketenimine intermediates generated by coupling reaction of alkenyldiazoacetates with o-alkenyl arylisocyanides undergo intramolecular [4 + 2] cycloaddition and provide a novel, highly efficient method for the one-step synthesis of carbazoles by formation of three new bonds and two rings from readily available acyclic starting materials.
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http://dx.doi.org/10.1021/acs.orglett.9b00307DOI Listing
May 2019

Ginsenoside Rk1 ameliorates paracetamol-induced hepatotoxicity in mice through inhibition of inflammation, oxidative stress, nitrative stress and apoptosis.

J Ginseng Res 2019 Jan 25;43(1):10-19. Epub 2017 Jul 25.

Institute of Special Wild Economic Animals and Plant, CAAS, Changchun, China.

Background: Frequent overdose of paracetamol (APAP) has become the major cause of acute liver injury. The present study was designed to evaluate the potential protective effects of ginsenoside Rk1 on APAP-induced hepatotoxicity and investigate the underlying mechanisms for the first time.

Methods: Mice were treated with Rk1 (10 mg/kg or 20 mg/kg) by oral gavage once per d for 7 d. On the 7th d, all mice treated with 250 mg/kg APAP exhibited severe liver injury after 24 h, and hepatotoxicity was assessed.

Results: Our results showed that pretreatment with Rk1 significantly decreased the levels of serum alanine aminotransferase, aspartate aminotransferase, tumor necrosis factor, and interleukin-1β compared with the APAP group. Meanwhile, hepatic antioxidants, including superoxide dismutase and glutathione, were elevated compared with the APAP group. In contrast, a significant decrease in levels of the lipid peroxidation product malondialdehyde was observed in the ginsenoside Rk1-treated group compared with the APAP group. These effects were associated with a significant increase of cytochrome P450 E1 and 4-hydroxynonenal levels in liver tissues. Moreover, ginsenoside Rk1 supplementation suppressed activation of apoptotic pathways by increasing Bcl-2 and decreasing Bax protein expression levels, which was shown using western blotting analysis. Histopathological observation also revealed that ginsenoside Rk1 pretreatment significantly reversed APAP-induced necrosis and inflammatory infiltration in liver tissues. Biological indicators of nitrative stress, such as 3-nitrotyrosine, were also inhibited after pretreatment with Rk1 compared with the APAP group.

Conclusion: The results clearly suggest that the underlying molecular mechanisms in the hepatoprotection of ginsenoside Rk1 in APAP-induced hepatotoxicity may be due to its antioxidation, antiapoptosis, anti-inflammation, and antinitrative effects.
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http://dx.doi.org/10.1016/j.jgr.2017.07.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6323149PMC
January 2019

Antimicrobial susceptibility and molecular typing of Pasteurella multocida isolated from six provinces in China.

Trop Anim Health Prod 2019 May 10;51(4):987-992. Epub 2018 Dec 10.

Department of Basic Veterinary Medicine, College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, Jilin, China.

Pasteurella multocida (P. multocida) is an important pathogen that causes bovine respiratory disease (BRD) in China and other countries. To investigate the antimicrobial susceptibility of P. multocida isolated from different provinces in China, we analyzed antimicrobial susceptibility phenotypes and pulsed-field gel electrophoresis (PFGE) types of P. multocida; then, we sequenced the complete genome of strain found to be multidrug-resistant. The isolates exhibited resistance to many antimicrobial agents, especially amikacin, sulfamethoxazole, sulfachloropyridazinesodium, macrolides, and fluoroquinolones. Pulsed-field gel electrophoresis analysis showed that a clonal spread of multidrug-resistant isolates occurred in various provinces. All of the isolates carried class I integron.
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http://dx.doi.org/10.1007/s11250-018-1754-9DOI Listing
May 2019

Therapeutic Effects of Tangshen Formula on Diabetic Nephropathy in Mice Using Cytokine Antibody Array.

J Diabetes Res 2018 22;2018:8237590. Epub 2018 Feb 22.

Beijing Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing, China.

Objective: Cytokines are essential promoters in the pathogenesis of diabetic nephropathy (DN) in type 2 diabetes. The following study investigates the adjustment mechanism of formula (TSF) on cytokine expressions in mice (DN animal model).

Materials And Methods: mice were randomly divided into three groups. The treated groups were orally administered with TSF and losartan for 12 weeks. Biochemical and histological examinations were determined at 8 and 12 weeks posttreatment, while the cytokine antibody array analysis was applied to analyze the expression of 144 cytokines in kidney tissues at the end of the 12th week posttreatment.

Results: TSF significantly reduced urinary albumin excretion and the levels of blood glucose, cholesterol, triglyceride, creatinine, and urea nitrogen. Furthermore, a significant decrease in glomerulus and mesangial area, as well as the downregulation of 24 cytokines and upregulated expressions of 5 cytokines, was found in the TSF-treated mice.

Conclusions: The present study reveals that TSF could ameliorate the metabolic anomalies and renal injury in db/db mice. One of the important mechanisms for treatment of DN using the treatment of TSF is the control of the JAK/STAT signaling pathway via regulation of IL-2, IL-6, IL-13, Il-15, and IFN- expression.
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http://dx.doi.org/10.1155/2018/8237590DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5842734PMC
September 2018

Pneumorrhachis: an under-recognized entity correlates with severity of spontaneous pneumomediastinum.

J Thorac Dis 2018 Feb;10(2):E149-E151

Department of Radiology, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung.

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http://dx.doi.org/10.21037/jtd.2018.01.65DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5864694PMC
February 2018

Tandem Lewis Pair Polymerization and Organocatalytic Ring-Opening Polymerization for Synthesizing Block and Brush Copolymers.

Molecules 2018 Feb 21;23(2). Epub 2018 Feb 21.

State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, Dalian, 116024, China.

Lewis pair polymerization is a powerful method for preparing soluble polymers bearing pendant active vinyl groups by directly polymerizing dissymmetric divinyl polar monomers. Herein, we present a strategy for synthesizing block and brush copolymers via tandem Lewis pair polymerization of methacrylates, "thiol-ene" click reaction and organocatalytic ring-opening polymerization of lactide.
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http://dx.doi.org/10.3390/molecules23020468DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6017872PMC
February 2018

Synergistic effect of eugenol with Colistin against clinical isolated Colistin-resistant strains.

Antimicrob Resist Infect Control 2018 29;7:17. Epub 2018 Jan 29.

College of Animal Science and Technology, Jilin Agricultural University, Xincheng Street No.2888, Changchun, 130118 China.

Background: Bacterial infections have become more challenging to treat due to the emergence of multidrug-resistant pathogenic bacteria. Combined antibiotics prove to be a relatively effective method to control such resistant strains. This study aim to investigate synergistic activity of eugenol combined with colistin against a collection of clinical isolated () strains, and to evaluate potential interaction.

Methods: Antimicrobial susceptibility, minimum inhibitory concentration (MIC) and fractional inhibitory concentration index (FICI) of the bacteria were determined by disk diffusion assay, broth microdilution method and checkerboard assay, respectively. The mcr-1 mRNA expression was measured by Real-time PCR. To predict possible interactions between eugenol and MCR-1, molecular docking assay was taken.

Results: For total fourteen strains including eight colistin-resistant strains, eugenol was determined with MIC values of 4 to 8 μg/mL. Checkerboard dilution test suggested that eugenol exhibited synergistic activity when combined with colistin (FICI ranging from 0.375 to 0.625). Comparison analysis of Real-time PCR showed that synergy could significantly down-regulate expression of mcr-1 gene. A metal ion coordination bond with catalytic zinc atom and a hydrogen bond with crucial amino acid residue Ser284 of MCR-1 were observed after molecular docking, indicating antibacterial activity and direct molecular interactions of eugenol with MCR-1 protein.

Conclusions: Our results demonstrated that eugenol exhibited synergistic effect with colistin and enhanced its antimicrobial activity. This might further contribute to the antibacterial actions against colistin-resistant strains.
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http://dx.doi.org/10.1186/s13756-018-0303-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5789579PMC
June 2019

The complete chloroplast genome sequence of and its phylogenetic analysis.

Mitochondrial DNA B Resour 2017 Dec 8;2(2):932-933. Epub 2017 Dec 8.

State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing, China.

The complete chloroplast genome of was reconstructed by reference-based assembly using whole-genome sequencing data. The total chloroplast genome size of was 158,080 bp in length, including a pair of inverted repeat regions (IRs) of 27,749 bp each, a large single-copy region (LSC) of 85,949 bp and a small single-copy region (SSC) of 16,633 bp. A total of 133 genes were predicted from the chloroplast genome, including 86 protein-coding genes, 39 tRNA genes and eight rRNA genes. Among these genes, 20 genes occurred in IRs, containing nine protein-coding genes, seven tRNA genes and four rRNA genes. The GC content of chloroplast genome was 36.6%. The phylogenetic analysis with 15 other species showed that was closely clustered with . The complete chloroplast genome of provides new insights into evolutionary and genomic studies.
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http://dx.doi.org/10.1080/23802359.2017.1413291DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7799498PMC
December 2017

Hsa_circ_0101996 combined with hsa_circ_0101119 in peripheral whole blood can serve as the potential biomarkers for human cervical squamous cell carcinoma.

Int J Clin Exp Pathol 2017 1;10(12):11924-11931. Epub 2017 Dec 1.

Department of Oncology, Chongqing Cancer Institute & Hospital & Cancer Center Chongqing, China.

Background: Previous study suggests changes in circRNAs in tumor tissues from cervical squamous cell carcinoma (CSCC) patients. However, little is known about the diagnostic value of circRNAs in CSCC. To assess the potential application of circRNAs as diagnostic tools in CSCC, the circulating circRNAs in peripheral whole blood were carried out.

Methods: Five up-regulated circRNAs in peripheral whole blood from 87 patients with CSCC and 55 healthy controls were first identified by real-time quantitative polymerase chain reaction (RT-qPCR). The diagnostic value was evaluated using receiver operating characteristics (ROC) curves and the area under the ROC curves (AUC).

Results: Compared with healthy controls, hsa_circ_0101996, hsa_circ_0104649, hsa_circ_0104443 and hsa_circ_0101119 expression were significantly up-regulated in peripheral whole blood from CSCC patients. ROC analysis showed that hsa_circ_0101996 and hsa_circ_0101119 could distinguish CSCC patients from healthy controls with high AUC (0.906 and 0.887, respectively). Intriguingly, the combination of hsa_circ_0101996 and hsa_circ_0101119 markedly improved AUC (0.964).

Conclusion: All of the findings suggest that hsa_circ_0101996 combined with hsa_circ_0101119 can serve as potential biomarkers for CSCC detection.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6966017PMC
December 2017

[Effect of processed Polygonum multiflorum on mRNA expression level of five subtypes of CYP450 enzymes in rat liver].

Zhongguo Zhong Yao Za Zhi 2017 Jan;42(2):352-356

School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China.

To observe the effect of processed Polygonum multiflorum on mRNA expression levels of five subtypes of CYP450 enzymes in rat liver. SD rats were randomly divided into the normal control group, processed P. multiflorum high dose and low dose groups (5.40 g•kg⁻¹ and 1.08 g•kg⁻¹). The rats in administration groups were continuously given with processed P. mutiflorum for 7 days by ig administration, and the rats in normal control group were given with the same volume of distilled water. After successive administration of 7 days, the serum biochemical indications were detected, and Real-time quantitative PCR technology was used to detect the mRNA expression levels of five subtypes of CYP450 enzymes in rat liver. Experimental results showed that AST was decreased significantly in both low and high dose groups. ALT was significantly decreased in low dose group and significantly increased in high dose group. The mRNA expression levels of five subtypes of CYP450 enzymes in rat liver were decreased in high dose and low dose groups in a dose-dependent manner. Especially the high dose processed P. multiflorum could significantly inhibit CYP1A2 and CYP2E1 mRNA expression levels in rats. The study showed that high dose P. multiflorum water extract had hepatotoxicity, and the degree of liver damage was increased with the increase of dose. It shall be noted that 5.40 g•kg⁻¹ water extract of P. multiflorum could significantly inhibit CYP1A2 and CYP2E1 mRNA expression levels in the liver of rats.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20161222.079DOI Listing
January 2017