Publications by authors named "Yi-Fan Wang"

112 Publications

Engineering Microdomains of Oxides in High-Entropy Alloy Electrodes toward Efficient Oxygen Evolution.

Adv Mater 2021 Jul 11:e2101845. Epub 2021 Jul 11.

Wuhan National High Magnetic Field Center & School of Physic, Huazhong University of Science and Technology, Wuhan, 430074, P. R. China.

One important goal of the current electrocatalysis is to develop integrated electrodes from the atomic level design to multilevel structural engineering in simple ways and low prices. Here, a series of oxygen micro-alloyed high-entropy alloys (O-HEAs) is developed via a metallurgy approach. A (CrFeCoNi) O bulk O-HEA shows exceptional electrocatalytic performance for the oxygen evolution reaction (OER), reaching an overpotential as low as 196 mV and a Tafel slope of 29 mV dec , and with stability longer than 120 h in 1 m KOH solution at a current density of 10 mA cm . It is shown that the enhanced OER performance can be attributed to the formation of island-like Cr O microdomains, the leaching of Cr ions, and structural amorphization at the interfaces of the domains. These findings offer a technological-orientated strategy to integrated electrodes.
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http://dx.doi.org/10.1002/adma.202101845DOI Listing
July 2021

Nitrogen fertilizer affects rhizosphere Cd re-mobilization by mediating gene AmALM2 and AmALMT7 expression in edible amaranth roots.

J Hazard Mater 2021 Jun 6;418:126310. Epub 2021 Jun 6.

Key Laboratory of Environmental Pollution and Health of Guangdong Province, School of Environment, Jinan University, Guangzhou 510632, China. Electronic address:

In-situ stabilization of Cd-contaminated farmland is a commonly used remediation technology. Yet, rhizosphere metabolites (e.g., organic acids) during crop cultivation may cause Cd re-mobilization and over-accumulation. Here, we identified four pivotal cytomembrane-localized genes underlying Cd accumulation difference between two contrasting edible amaranth cultivars based on root gene expression profile, studied their subcellular localization and functional characteristics, and then investigated effects of nitrogen fertilizer on their expression and rhizosphere Cd re-mobilization. Results showed that more Cd accumulated by edible amaranth was due to rhizosphere Cd mobilization by mediating high expression of AmALMT2 and AmALMT7 genes, not Cd transporters in roots. This was confirmed by heterologous expression of AmALMT2 and AmALMT7 genes in Arabidopsis thaliana, since they mediated malic, fumaric, succinic, and aspartic acids efflux. Furthermore, nitrogen influencing rhizosphere acidification might be closely associated with organic acids efflux genes. Compared with N-NO application, N-NH was massively assimilated into glutamates and oxaloacetates through up-regulating glutamine synthetase and alanine-aspartate-glutamate metabolic pathways, thereby enhancing TCA cycle and organic acids efflux dominated by binary carboxylic acids via up-regulating AmALMT2 and AmALMT7 genes, which finally caused Cd re-mobilization. Therefore, N-NO-dominated nitrogen retarded rhizosphere Cd re-mobilization via inhibiting organic acids efflux function of AmALMT2 and AmALMT7 proteins.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126310DOI Listing
June 2021

Tanshinone I, a new EZH2 inhibitor restricts normal and malignant hematopoiesis through upregulation of and .

Theranostics 2021 8;11(14):6891-6904. Epub 2021 May 8.

Women's Hospital, and Institute of Genetics, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.

Tanshinone, a type of diterpenes derived from , is a particularly promising herbal medicine compound for the treatment of cancers including acute myeloid leukemia (AML). However, the therapeutic function and the underlying mechanism of Tanshinone in AML are not clear, and the toxic effect of Tanshinone limits its clinical application. Our work utilizes human leukemia cell lines, zebrafish transgenics and xenograft models to study the cellular and molecular mechanisms of how Tanshinone affects normal and abnormal hematopoiesis. WISH, Sudan Black and O-Dianisidine Staining were used to determine the expression of hematopoietic genes on zebrafish embryos. RNA-seq analysis showed that differential expression genes and enrichment gene signature with Tan I treatment. The surface plasmon resonance (SPR) method was used with a BIAcore T200 (GE Healthcare) to measure the binding affinities of Tan I. methyltransferase assay was performed to verify Tan I inhibits the histone enzymatic activity of the PRC2 complex. ChIP-qPCR assay was used to determine the H3K27me3 level of EZH2 target genes. We found that Tanshinone I (Tan I), one of the Tanshinones, can inhibit the proliferation of human leukemia cells and in the xenograft zebrafish model, as well as the normal and malignant definitive hematopoiesis in zebrafish. Mechanistic studies illustrate that Tan I regulates normal and malignant hematopoiesis through direct binding to EZH2, a well-known histone H3K27 methyltransferase, and inhibiting PRC2 enzymatic activity. Furthermore, we identified and as two possible downstream genes of Tan I's effects on EZH2. Together, this study confirmed that Tan I is a novel EZH2 inhibitor and suggested and as two potential therapeutic targets for myeloid malignant diseases.
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http://dx.doi.org/10.7150/thno.53170DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8171091PMC
May 2021

Stereodivergent Synthesis of Tertiary Fluoride-Tethered Allenes via Copper and Palladium Dual Catalysis.

J Am Chem Soc 2021 May 10;143(19):7285-7291. Epub 2021 May 10.

CAS Key Laboratory of Synthetic Chemistry of Natural Substances, Center for Excellence in Molecular Synthesis, Shanghai Institute of Organic Chemistry, University of Chinese Academy of Sciences, Shanghai 200032, China.

Herein we describe a protocol for the unprecedented stereodivergent synthesis of tertiary fluoride-tethered allenes bearing a stereogenic center and stereogenic axis via Cu/Pd synergistic catalysis. A broad scope of conjugated enynes are coupled with various α-fluoroesters in high yields with high diastereoselectivities and generally >99% ee. In addition, the four stereoisomers of the allene products ensure precise access to the corresponding four stereoisomers of the fluorinated hydrofurans via a novel stereodivergent axial-to-central chirality transfer process.
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http://dx.doi.org/10.1021/jacs.1c03157DOI Listing
May 2021

The complete mitochondrial genome of the freshwater crab Türkay and Dai 1997 (Decapoda: Brachyura: Potamoidea).

Mitochondrial DNA B Resour 2021 Apr 26;6(4):1554-1556. Epub 2021 Apr 26.

School of Basic Medical Sciences, Research lab of Freshwater Crustacean Decapoda & Paragonimus, Nanchang University, Nanchang, PR China.

We report the complete mitochondrial genome of for the first time, which is found to be 17,612 base pairs in length, and contains 13 protein-coding genes (PCGs), 2 ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA), and 1 non-coding AT-rich region known as the D-loop. In addition, the mitogenome has 17 intergenic regions ranging from 1 to 1512 bp in length. The mitochondrial genome of is the first mitochondrial genome under the genus , providing DNA data for species identification, enriching the species diversity of Brachyura. The maximum-likelihood (ML) tree and Bayesian inference (BI) tree based on the 13 PCGs of mitochondrial genome of Brachyura species showed similar topologies with high confidence, and the analysis results were consistent with the current mainstream classification system. The results indicating that is closely related to , , and , and it is likely to be derived from them.
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http://dx.doi.org/10.1080/23802359.2021.1915208DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079082PMC
April 2021

Chromosome-scale assembly of the Kandelia obovata genome.

Hortic Res 2020 May 2;7(1):75. Epub 2020 May 2.

Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at the College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

The mangrove Kandelia obovata (Rhizophoraceae) is an important coastal shelterbelt and landscape tree distributed in tropical and subtropical areas across East Asia and Southeast Asia. Herein, a chromosome-level reference genome of K. obovata based on PacBio, Illumina, and Hi-C data is reported. The high-quality assembled genome size is 177.99 Mb, with a contig N50 value of 5.74 Mb. A large number of contracted gene families and a small number of expanded gene families, as well as a small number of repeated sequences, may account for the small K. obovata genome. We found that K. obovata experienced two whole-genome polyploidization events: one whole-genome duplication shared with other Rhizophoreae and one shared with most eudicots (γ event). We confidently annotated 19,138 protein-coding genes in K. obovata and identified the MADS-box gene class and the RPW8 gene class, which might be related to flowering and resistance to powdery mildew in K. obovata and Rhizophora apiculata, respectively. The reference K. obovata genome described here will be very useful for further molecular elucidation of various traits, the breeding of this coastal shelterbelt species, and evolutionary studies with related taxa.
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http://dx.doi.org/10.1038/s41438-020-0300-xDOI Listing
May 2020

Facile Strategy for Efficient Charge Separation and High Photoactivity of Mixed-Linker MOFs.

ACS Appl Mater Interfaces 2021 May 25;13(17):20897-20905. Epub 2021 Apr 25.

Beijing Key Laboratory for Optical Materials and Photonic Devices, Department of Chemistry, Capital Normal University, Beijing 100048, China.

Two new sets of UiO-Zr metal-organic framework (MOF) bearing mixed linkers and that have different band gaps and edges were prepared through post oxidation and direct methods, namely, ( = 4, 9, 12 oxidation hours) and ( = 0, 0.4, 0.6, 2), respectively. These composites with stoichiometric components were fully characterized via proton nuclear magnetic resonance (H NMR) spectroscopy, powder X-ray diffraction (PXRD), transmission electron microscopy (TEM), Fourier-transform infrared (FT-IR) spectra, Brunauer-Emmett-Teller (BET), photo electrochemical measurements, and femtosecond transient absorption (fs-TA) spectroscopy. The structure, electronic property, and photoresponsive and catalytic ability as the functions of the molar ratio of linkers and the synthetic protocol were first investigated. The mixed-linker and exhibited improved performances as compared to the and possessing neat linkers only. Their photo response and catalytic activity varied with different linker ratios. For , the performance increased with the increasing linker ratio upon oxidation but reached the highest as the being of 24.4% in . In comparison, the best photocurrent (80.74 uA/cm) and the highest H generation rate (2018.8 μmol g h) (λ > 400 nm) in are about twice those of obtained by direct synthesis, although the linker ratio of those two composites is almost the same (24.4% vs 23.9%). Recorded shorter lifetime and higher charge separation efficiency of the former than those of the latter suggest the postsynthetic protocol as the efficient method for achieving the mixed-liner-MOF-based photocatalyst with high performance. A new type-II tailored homojunction is proposed in these mixed-linker MOFs for their efficient charge separation and improved activity.
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http://dx.doi.org/10.1021/acsami.1c04130DOI Listing
May 2021

Rational engineering of BaLal_16 from a novel Bacillus amyloliquefaciens strain to improve catalytic performance.

Enzyme Microb Technol 2021 May 18;146:109781. Epub 2021 Mar 18.

School of Biological Science, Jining Medical University, 669 Xueyuan Road, Rizhao, 276800, China. Electronic address:

-amino acid ligases (Lals) are promising biocatalysts for the synthesis of dipeptides with special biological properties. However, their poor (or broad) substrate specificity limits their industrial applications. To address this problem, a molecular engineering method for Lals was developed to enhance their catalytic performance. Based on substrate channeling, entrances to the active site for different substrates were identified, and the "gate" located around the active site pocket, which plays an essential role in substrate recognition, was then engineered to facilitate acceptance of -Gln. Two mutants (L110Y and N108F/L110Y) were discovered to display significantly increased catalytic activity toward -Ala and -Gln in the biosynthesis of Ala-Gln. The catalytic efficiency (k/ K) of the L110Y and N108F/L110Y mutants was improved by 2.64-fold and 4.06-fold, respectively, compared with that of the wild type. N108F/L110Y was then further applied for batch production of Ala-Gln, which showed that the released Pi yield was 694.47 μM, which was an increase of approximately 21.4 %, and the yield of Ala-Gln was approximately 2.59 mM L mg. Collectively, these findings suggest the potential practical application of this method in the rational design of Lals for increased catalytic performance.
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http://dx.doi.org/10.1016/j.enzmictec.2021.109781DOI Listing
May 2021

The gut microbiota metabolite capsiate promotes Gpx4 expression by activating to inhibit intestinal ischemia reperfusion-induced ferroptosis.

Gut Microbes 2021 Jan-Dec;13(1):1-21

Department of Anesthesiology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.

Ferroptosis, a new type of cell death has been found to aggravate intestinal ischemia/reperfusion (I/R) injury. However, little is known about the changes of gut microbiota and metabolites in intestinal I/R and the role of gut microbiota metabolites on ferroptosis-induced intestinal I/R injury. This study aimed to establish a mouse intestinal I/R model and ileum organoid hypoxia/reoxygenation (H/R) model to explore the changes of the gut microbiota and metabolites during intestinal I/R and protective ability of capsiate (CAT) against ferroptosis-dependent intestinal I/R injury. Intestinal I/R induced disturbance of gut microbiota and significant changes in metabolites. We found that CAT is a metabolite of the gut microbiota and that CAT levels in the preoperative stool of patients undergoing cardiopulmonary bypass were negatively correlated with intestinal I/R injury. Furthermore, CAT reduced ferroptosis-dependent intestinal I/R injury in vivo and in vitro. However, the protective effects of CAT against ferroptosis-dependent intestinal I/R injury were abolished by RSL3, an inhibitor of glutathione peroxidase 4 (Gpx4), which is a negative regulator of ferroptosis. We also found that the ability of CAT to promote Gpx4 expression and inhibit ferroptosis-dependent intestinal I/R injury was abrogated by JNJ-17203212, an antagonist of transient receptor potential cation channel subfamily V member 1 (TRPV1). This study suggests that the gut microbiota metabolite CAT enhances Gpx4 expression and inhibits ferroptosis by activating TRPV1 in intestinal I/R injury, providing a potential avenue for the management of intestinal I/R injury.
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http://dx.doi.org/10.1080/19490976.2021.1902719DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009132PMC
March 2021

[Comparative analysis of binding multi-fold rib graft, iliac bone graft and titanium mesh graft during surgery of tuberculosis of thoracic vertebra].

Zhongguo Gu Shang 2021 Jan;34(1):73-80

Zhejiang Integrated Traditional Chinese and Western Medicine Hospital, Hangzhou 310000, Zhejiang, China.

Objective: To compare the clinical effects of three different methods of binding multi-fold rib graft, iliac bone graft and titanium mesh graft in tuberculosis of thoracic vertebra by approach of transverse rib process.

Methods: A hundred and seven patients with tuberculosis of thoracic vertebra received surgical treatment from January 2010 to December 2016 were retrospectively analyzed. The patients were divided into three groups according different methods of bone graft. The surgical approach of the transverse rib process was used in all 107 patients, after thoroughly remove the necrotic tissue of tuberculosis, three different bone grafts were used respectively including iliac bone graft (36 cases, group A), binding multi-fold rib graft (35 cases, group B), titanium mesh bone graft (36 cases, group C). Perioperative indexes, the time required for bone graft during operation, intraoperation blood loss, the loss rate of the anterior edge of the lesion, Cobb angle, postoperative bone graft fusion time, spinal nerve recovery and Oswestry Disability Index were compared among three groups.

Results: All the patients were followed up for 13 to 24 months, and the operation time required for bone graft was (23.2±4.1) min in group A, (23.8± 4.4)min in group B, and (25.5±4.2) min in group C, with no statistically significant difference among three groups (>0.05). Intraoperative blood loss was (541.6±35.3) ml in group A, (546.8±27.8) ml in group B, and (540.1±34.5) ml in group C, withno statistically significant difference among three groups(>0.05). Preoperative anterior vertebral height loss rate was (46.0± 3.1)% in group A, (46.4±3.3)% in group B, and (45.3±3.6)% in group B;at the final follow up, the loss rate of anterior vertebral height among three groups was (8.6±5.0)%, (8.1±4.2)%, (9.4±4.3)%, respectively. There were no statistically significant differences before operation and final follow-up among three groups (>0.05). Preoperative Cobb angle was (35.1±4.8)° in group A, (35.2±4.5)° in group B and (35.2±4.5)° in group C, with no statistically significant difference among three groups (>0.05);postoperative at 3 days, Cobb angle in three groups was (15.1±3.6)°, (15.3±3.1)° and (15.2±3.4)°, respectively, there was no statistically significant difference among three groups (>0.05);at the final follow-up, the Cobb angle among three groups was (17.7±3.3)°, (17.9±3.9)°, (18.6±3.6)°, respectively, with no statistically significant difference among three groups (>0.05). The time of bone graft fusion was (5.6±0.5) months in group A, (5.6±0.6) months in group B and (5.8±0.6)months in group C, with no statistically significant difference among three groups (>0.05). Frankel classification at the final follow up, 4 cases were grade B, 7 cases were grade C, 10 cases were grade D, and 86 cases were grade E. Spinal nerve function in all three groups recovered to a certain extent after treatment, with no statistically significant difference among three groups (> 0.05). Oswestry Disability Index at the final follow-up showed no statistically significant difference among three groups (> 0.05).

Conclusion: The approach of transverse rib process for debridement of lesions can effectively treat tuberculosis of thoracic vertebra by binding multi-fold rib graft, iliac bone graft and titanium mesh graft, but binding multi-fold rib graft can effectively avoid iliac bone donor complications, and is an effective alternative to iliac bone graft, which is worth popularizing.
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http://dx.doi.org/10.12200/j.issn.1003-0034.2021.01.014DOI Listing
January 2021

Impact of Implementing CYP2C19 Genotype-Guided Antiplatelet Therapy on P2Y Inhibitor Selection and Clinical Outcomes in Acute Coronary Syndrome Patients After Percutaneous Coronary Intervention: A Real-World Study in China.

Front Pharmacol 2020 20;11:582929. Epub 2021 Jan 20.

Department of Pharmacy, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.

CYP2C19 loss-of-function (LOF) alleles reduce the effectiveness of clopidogrel in patients undergoing percutaneous coronary intervention for acute coronary syndrome. However, the clinical impact of implementing CYP2C19 gene-guided pharmacotherapy is unclear, especially among the Chinese population. The purpose of this study was to evaluate P2Y12 receptor inhibitor selection and clinical outcomes upon implementation of CYP2C19 genotype-guided pharmacotherapy in current clinical practice. This was a single-center observational cohort study. Adult percutaneous coronary intervention patients who received CYP2C19 genetic testing (*2, *3, *17 alleles) were included. Ticagrelor was recommended for patients with a LOF allele. Factors related to P2Y12 inhibitor selection were determined by logistic regression. The primary endpoint was major cardiac or cerebrovascular adverse events (MACCE) within 12 months. MACCE and clinically significant bleeding events (BARC ≥2) in the LOF-clopidogrel group, non-LOF-clopidogrel group, and non-LOF-ticagrelor group were compared with those in the LOF-ticagrelor group. The inverse probability of treatment weighting (IPTW) was adjusted in a Cox regression analysis to eliminate confounding factors. Among 1,361 patients, 826 (60.7%) had a LOF allele. Patients with a LOF allele were more likely to be prescribed ticagrelor (multivariate-adjusted OR 1.349; 95% CI 1.040 to 1.751; = 0.024). The MACCE rate was higher in the LOF-clopidogrel group than in the LOF-ticagrelor group (7.8 vs. 4.0%; log-rank = 0.029; IPTW-adjusted HR 2.138; 95% CI 1.300-3.515). Compared with the LOF-ticagrelor group, the non-LOF-clopidogrel group showed no significant difference in MACCE rate (5.8 vs. 4.0%; log-rank = 0.272; IPTW-adjusted HR 1.531; 95% CI 0.864-2.714). Among the patients treated with ticagrelor, there was no significant difference in the MACCE rate between the LOF group and non-LOF group (4.3 vs. 4.0%; log-rank = 0.846; IPTW-adjusted HR 1.184; 95% CI 0.582-2.410). There was no significant difference in the incidence of clinically significant bleeding events among the four groups. This study confirms that efficiently returned CYP2C19 genotype results did partially guide cardiologists to prescribe ticagrelor for patients with a LOF allele, and that clopidogrel had a higher risk of MACCE than ticagrelor in these patients, which provides support for the implementation of CYP2C19 gene-guided antiplatelet therapy in clinical practice.
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http://dx.doi.org/10.3389/fphar.2020.582929DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7854467PMC
January 2021

Search for Gravitational Waves from High-Mass-Ratio Compact-Binary Mergers of Stellar Mass and Subsolar Mass Black Holes.

Phys Rev Lett 2021 Jan;126(2):021103

Max-Planck-Institut für Gravitationsphysik (Albert-Einstein-Institut), D-30167 Hannover, Germany and Leibniz Universität Hannover, D-30167 Hannover, Germany.

We present the first search for gravitational waves from the coalescence of stellar mass and subsolar mass black holes with masses between 20-100  M_{⊙} and 0.01-1  M_{⊙}(10-10^{3}  M_{J}), respectively. The observation of a single subsolar mass black hole would establish the existence of primordial black holes and a possible component of dark matter. We search the ∼164 day of public LIGO data from 2015-2017 when LIGO-Hanford and LIGO-Livingston were simultaneously observing. We find no significant candidate gravitational-wave signals. Using this nondetection, we place a 90% upper limit on the rate of 30-0.01  M_{⊙} and 30-0.1  M_{⊙} mergers at <1.2×10^{6} and <1.6×10^{4}  Gpc^{-3}  yr^{-1}, respectively. If we consider binary formation through direct gravitational-wave braking, this kind of merger would be exceedingly rare if only the lighter black hole were primordial in origin (<10^{-4}  Gpc^{-3}  yr^{-1}). If both black holes are primordial in origin, we constrain the contribution of 1(0.1)M_{⊙} black holes to dark matter to <0.3(3)%.
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http://dx.doi.org/10.1103/PhysRevLett.126.021103DOI Listing
January 2021

Progress on genic male sterility gene in soybean.

Yi Chuan 2021 Jan;43(1):52-65

School of Life Sciences, Guangzhou University, Guangzhou 510006, China.

Male sterility refers to the phenomenon that stamens cannot grow normally and produce viable pollen grains in plants. Hybrid seed production by taking advantage of the trait of male sterility is an effective and quick strategy to increase crop yield. Up to date, the yield of rice (Oryza sativa L.), maize (Zea mays L.), wheat (Triticum aestivum L.) and other crops has been greatly increased based on hybrid vigor utilization. Soybean (Glycine max (L.) Merr.) is a self-pollination species, artificial emasculation is not only time-consuming, but also labor-intensive and economically impracticable. So far, large scale hybrid breeding has not been performed in soybean due to the shortage of male sterile lines suitable for hybrid production. Therefore, it is urgent to identify a stable male sterile system for the rapid utilization of heterosis in soybean. In this review, we summarize the progress on the discovery of soybean genic male sterility (GMS) mutants and GMS genes. Combining with the investigation of GMS genes in Arabidopsis, rice and maize, we provide important insights into the identification and potential utilization of GMS genes in soybean in the perspective of reverse genetics.
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http://dx.doi.org/10.16288/j.yczz.20-357DOI Listing
January 2021

The complete chloroplast genome sequence of (Staphyleaceae).

Mitochondrial DNA B Resour 2020 Sep 21;5(3):3354-3356. Epub 2020 Sep 21.

College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, P. R. China.

The Rosids are characterized by remarkable morphological and ecological diversity. Here, we provide the completed plasmid genome of The complete chloroplast size of is 160,111 bp, including a large single-copy (LSC) region of 89,631 bp, a small single-copy (SSC) region of 18,247 bp, a pair of invert repeats (IR) regions of 26,120 bp. Plastid genome contains 131 genes, 86 protein-coding genes, 37 genes, and eight genes. Phylogenetic analysis base on 23 plastid genomes indicates that is clustered with the plants of the Euscaphis japonica and .
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http://dx.doi.org/10.1080/23802359.2020.1821821DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7783033PMC
September 2020

The complete chloroplast genome sequence of (Staphyleaceae).

Mitochondrial DNA B Resour 2020 Jul 6;5(3):2658-2660. Epub 2020 Jul 6.

College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, China.

, belong to Tapisciaceae, is endangered tree endemic to China. Here, we provide the complete plastid genomic data of with the aim of providing data for future conservation efforts research and revealing its phylogenetic position. The complete chloroplast sequence is 161,093 bp, including a large single-copy (LSC) region of 87,782 bp, a small single-copy (SSC) region of 18,517 bp, a pair of invert repeats (IR) regions of 27,387 bp. Plastid genome contains 131 genes, 85 protein-coding genes, 38 tRNA genes, and eight rRNA genes. Phylogenetic analysis base on 19 plastid genomes indicates that located Malvids branch, and is more closely related to the species of the order Sapindales than those of the order Malvales.
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http://dx.doi.org/10.1080/23802359.2020.1781565DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7781918PMC
July 2020

Light Microscopic and Ultrastructural Characteristics of Heterophil Toxicity and Left-shifting in Green Sea Turtles () from Taiwan.

Zool Stud 2020 10;59:e52. Epub 2020 May 10.

Institute of Veterinary Clinical Science, School of Veterinary Medicine, National Taiwan University, No.153, Sec. 3, Keelung Rd, Da-an district, Taipei 10672, Taiwan. E-mail: (Yu); (Wang); (Chi).

Heterophil toxic change (TC) and left-shifting (LS) are widely used as indicators of accelerated granulopoiesis. However, the ultrastructure of heterophil TC and LS in sea turtles remain poorly understood. This study aimed to describe the ultrastructural characteristics of sea turtle TC and LS heterophils, compare the staining quality of accessible staining methods, and provide a better understanding of the clinical applications and limitations of heterophil TC and LS examinations. Blood samples were collected from 21 rescued sea turtles from January 2017 to September 2018. Morphologic ( = 22) and ultrastructural ( = 15) examination of TC and LS heterophils were performed, and the qualities of three staining methods (Wright-Giemsa stain, Diff-Quik stain and Liu's stain) were analyzed to diagnose TC and LS heterophils. In addition, the diagnostic values of TC and LS heterophils were examined. Diff-Quik stain was significantly inferior in the assessment of heterophil TC and/or LS comparing to the Wright-Giemsa stain and Liu's stain (Mann-Whitney test, < 0.001). Microscopic examinations of heterophil TC and/or LS were comparable to transmission electron microscopy examinations (Cohen's kappa coefficient, κ = 1). The correlation between the presence of heterophil TC and/or LS and clinical inflammatory state was weak (Spearman's rank correlation coefficient, = 0.171, = 0.445). In conclusion, this is the first study to describe the ultrastructural characteristics of reptile TC and LS heterophils. Wright-Giemsa stain and Liu's stain were suitable staining methods for the microscopic observations of TC and LS heterophil in sea turtles. Given the poor correlation between TC and/or LS and clinical findings, TC and LS are not a suitable diagnostic indicator of green sea turtles' inflammation status.
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http://dx.doi.org/10.6620/ZS.2020.59-52DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7807173PMC
May 2020

Antimicrobial mechanism of alkyl gallates against Escherichia coli and Staphylococcus aureus and its combined effect with electrospun nanofibers on Chinese Taihu icefish preservation.

Food Chem 2021 Jun 27;346:128949. Epub 2020 Dec 27.

School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, Zhejiang 310035, China.

The objective of this study was to investigate the antibacterial activity and potential mechanism of alkyl gallates against Escherichia coli and Staphylococcus aureus. Results show that the length of the alkyl chain plays a pivotal role in eliciting the activity and octyl gallate (OG) exerted excellent bactericidal activity through a multiple bactericidal mechanism. OG functions against both bacteria through damaging bacterial cell wall integrity, permeating into cells and then interacting with DNA, as well as disturbing the activity of the respiratory electron transport chain to induce a high-level toxic ROS (hydroxyl radicals) generation and up-regulation of the ROS genes. Also, electrospun nanofibers with OG have unique superiorities for maintaining the freshness of the icefish (4 °C). This research not only provides a more in-depth understanding of the interaction between OG and microorganisms but also highlights the great promise of using OG as a safe multi-functionalized food additive for food preservations.
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http://dx.doi.org/10.1016/j.foodchem.2020.128949DOI Listing
June 2021

One-Pot Preparation of Tricyclo[5.2.2.0]undecanes via Cu-Catalyzed Asymmetric Carboboration of Cyclohexadienone-Tethered Allenes.

Org Lett 2021 01 30;23(2):607-611. Epub 2020 Dec 30.

The Research Center of Chiral Drugs, Innovation Research Institute of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, China.

The Cu-catalyzed asymmetric carboboration of cyclohexadienone-tethered allenes has been achieved through regioselective β-borylation of the allenes and subsequent conjugate addition to cyclohexadienones, affording -bicyclic frameworks with acceptable yields and high to excellent enantioselectivities. Further conjugate borylation of the carboboration products proved to be a favorable kinetic resolution process, which improved the overall enantioselectivity. Finally, one-pot preparation of highly enantioenriched tricyclo[5.2.2.0]undecanes was developed from the cyclohexadienone-tethered allenes through β-borylation/1,4-addition and subsequent tandem oxidation/intramolecular aldol reaction.
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http://dx.doi.org/10.1021/acs.orglett.0c04191DOI Listing
January 2021

Enhanced performance of microbial fuel cells using Ag nanoparticles modified Co, N co-doped carbon nanosheets as bifunctional cathode catalyst.

Bioelectrochemistry 2021 Apr 29;138:107717. Epub 2020 Nov 29.

School of Chemistry and Chemical Engineering/Guangzhou Key Laboratory for Clean Energy and Materials, Guangzhou University, Guangzhou 510006, China.

The slow kinetics of oxygen reduction reaction (ORR) and the formation of biofilm on cathode severely limited the performance of microbial fuel cells (MFCs). An efficient way to enhance the power-generation capacity and long-term stability of MFCs is to develop bifunctional catalyst by incorporating the efficient ORR catalysts with antibacterial ingredient. In this study, the Ag/Co-N-C nanosheets were designed and synthesized by decorating Ag nanoparticles (NPs) onto Co-N-C nanosheets, which were prepared from Zn/Co bimetallic metal-organic framework (ZIF-67/ZIF-8) precursor. The Zn/Co ratio, Ag doping amount and the calcination temperature of the precursor were systematically investigated. The optimum sample Ag/Co-N-C-30 revealed the excellent ORR performance with a half-wave potential of 0.80 V vs. RHE, which was slightly lower than that of Pt/C (0.82 V vs. RHE). The MFCs equipped with Ag/Co-N-C-30 cathode exhibited maximum power density of 548 ± 12.6 mW m and superior durability even after 1600 h operation. Besides, the selective antimicrobial ability of Ag/Co-N-C-30 was further explored and the aerobic bacteria in cathode biofilm was found to be obviously inhibited by Ag/Co-N-C-30. The results suggested the Ag/Co-N-C nanosheets can serve as a promising cathode catalyst for practical applications of MFCs.
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http://dx.doi.org/10.1016/j.bioelechem.2020.107717DOI Listing
April 2021

Recent advances in visual detection for cancer biomarkers and infectious pathogens.

J Mater Chem B 2021 01 23;9(1):35-52. Epub 2020 Nov 23.

Tongji School of Pharmacy, HuaZhong University of Science and Technology, Wuhan 430030, China.

It is an urgency to detect infectious pathogens or cancer biomarkers using rapid, simple, convenient and cost-effective methods in complex biological samples. Many existing approaches (traditional virus culture, ELISA or PCR) for the pathogen and biomarker assays face several challenges in the clinical applications that require lengthy time, sophisticated sample pre-treatment and expensive instruments. Due to the simple and rapid detection manner as well as no requirement of expensive equipment, many visual detection methods have been considered to resolve the aforementioned problems. Meanwhile, various new materials and colorimetric/fluorescent methods have been tried to construct new biosensors for infectious pathogens and biomarkers. However, the recent progress of these aspects is rarely reviewed, especially in terms of integration of new materials, microdevice and detection mechanism into the visual detection systems. Herein, we provide a broad field of view to discuss the recent progress in the visual detection of infectious pathogens and cancer biomarkers along with the detection mechanism, new materials, novel detection methods, special targets as well as multi-functional microdevices and systems. The novel visual approaches for the infectious pathogens and biomarkers, such as bioluminescence resonance energy transfer (BRET), metal-induced metallization and clustered regularly interspaced short palindromic repeats (CRISPR)-based biosensors, are discussed. Additionally, recent advancements in visual assays utilizing various new materials for proteins, nucleic acids, viruses, exosomes and small molecules are comprehensively reviewed. Future perspectives on the visual sensing systems for infectious pathogens and cancers are also proposed.
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http://dx.doi.org/10.1039/d0tb01883jDOI Listing
January 2021

Copper(I)-catalyzed diastereo- and enantio-selective construction of optically pure exocyclic allenes.

Nat Commun 2020 08 27;11(1):4293. Epub 2020 Aug 27.

The Research Center of Chiral Drugs, Innovation Research Institute of Traditional Chinese Medicine and China-Thailand Joint Research Institute of Natural Medicine, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai, 201203, China.

Among about 150 identified allenic natural products, the exocyclic allenes constitute a major subclass. Substantial efforts are devoted to the construction of axially chiral allenes, however, the strategies to prepare chiral exocyclic allenes are still rare. Herein, we show an efficient strategy for the asymmetric synthesis of chiral exocyclic allenes with the simultaneous control of axial and central chirality through copper(I)-catalyzed asymmetric intramolecular reductive coupling of 1,3-enynes to cyclohexadienones. This tandem reaction exhibits good functional group compatibility and the corresponding optically pure exocyclic allenes bearing cis-hydrobenzofuran, cis-hydroindole, and cis-hydroindene frameworks, are obtained with high yields (up to 99% yield), excellent diastereoselectivities (generally >20:1 dr) and enantioselectivities (mostly >99% ee). Furthermore, a gram-scale experiment and several synthetic transformations of the chiral exocyclic allenes are also presented.
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http://dx.doi.org/10.1038/s41467-020-18136-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7453021PMC
August 2020

The Recent Advances of Fluorescent Sensors Based on Molecularly Imprinted Fluorescent Nanoparticles for Pharmaceutical Analysis.

Curr Med Sci 2020 Jun 17;40(3):407-421. Epub 2020 Jul 17.

School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.

Fluorescent nanoparticles have good chemical stability and photostability, controllable optical properties and larger stokes shift. In light of their designability and functionability, the fluorescent nanoparticles are widely used as the fluorescent probes for diverse applications. To enhance the sensitivity and selectivity, the combination of the fluorescent nanoparticles with the molecularly imprinted polymer, i.e. molecularly imprinted fluorescent nanoparticles (MIFN), was an effective way. The sensor based on MIFN (the MIFN sensor) could be more compatible with the complex sample matrix, which was especially widely adopted in medical and biological analysis. In this mini-review, the construction method, detective mechanism and types of MIFN sensors are elaborated. The current applications of MIFN sensors in pharmaceutical analysis, including pesticides/herbicide, veterinary drugs/drugs residues and human related proteins, are highlighted based on the literature in the recent three years. Finally, the research prospect and development trend of the MIFN sensor are forecasted.
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http://dx.doi.org/10.1007/s11596-020-2195-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7366466PMC
June 2020

Inhibition of GSK3β and RIP1K Attenuates Glial Scar Formation Induced by Ischemic Stroke Reduction of Inflammatory Cytokine Production.

Front Pharmacol 2020 12;11:812. Epub 2020 Jun 12.

Jiangsu Key Laboratory of Neuropsychiatric Diseases and College of Pharmaceutical Sciences, Department of Pharmacology and Laboratory of Cerebrovascular Pharmacology, College of Pharmaceutical Science, Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases, School of Public Health, Soochow University, Suzhou, China.

In the chronic phase following ischemic stroke, glial scars can prevent axonal regeneration and the intensification of inflammation. The protective effect of inhibition of glycogen synthase kinase-3β (GSK3β) or receptor-interacting protein 1 kinase (RIP1K) on ischemic stroke has been previously reported. The current study examined the effects of RIP1K and GSK3β on ischemic stroke-induced glial scar formation. To investigate this, we used an model of ischemic stroke based on middle cerebral artery occlusion for 90 min followed by reperfusion for 7 d, and an model in primary cultured astrocytes involving oxygen and glucose deprivation for 6 h followed by reoxygenation for 24 h. Both and , we found that SB216763, a GSK3β inhibitor, and necrostatin-1 (Nec-1), a RIP1K inhibitor, decreased levels of glial scar markers, including glial fibrillary acidic protein (GFAP), neurocan, and phosphacan. SB216763 and Nec-1 also decreased levels of inflammatory related cytokines, including interleukin-6 (IL-6), interleukin-1 β (IL-1β), and tumor necrosis factor-α (TNF-α). However, only Nec-1 increased the level of interleukin-1 receptor antagonist. Concurrent neutralization of TNF-α, IL-1β, and IL-6 with their antibodies provided better reduction in oxygen and glucose deprivation-induced increases in scar markers than obtained with separate use of each antibody. Further investigations showed that SB216763 reduced the levels of necroptosis-related proteins, including RIP1K, p-RIP1K, RIP3K, p-RIP3K, mixed lineage kinase domain-like protein (MLKL), and p-MLKL, while Nec-1 decreased the expression of p-GSK3β. Compared with Nec-1 (10 μM) and SB216763 (1 μM) alone, Nec-1 and SB216763 in combination reduced levels of GFAP, neurocan, and inflammatory-related cytokines. In conclusion, inhibition of GSK3β or RIP1K reduced glial scar formation induced by ischemic stroke. The underlying mechanisms might be at least, partially related to reducing levels of inflammatory-related cytokines and to blocking an interaction between GSK3β- and RIP1K-mediated pathways.
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http://dx.doi.org/10.3389/fphar.2020.00812DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7303311PMC
June 2020

Deep Learning for Accurate Diagnosis of Liver Tumor Based on Magnetic Resonance Imaging and Clinical Data.

Front Oncol 2020 28;10:680. Epub 2020 May 28.

Department of General Surgery, School of Medicine, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou, China.

Early-stage diagnosis and treatment can improve survival rates of liver cancer patients. Dynamic contrast-enhanced MRI provides the most comprehensive information for differential diagnosis of liver tumors. However, MRI diagnosis is affected by subjective experience, so deep learning may supply a new diagnostic strategy. We used convolutional neural networks (CNNs) to develop a deep learning system (DLS) to classify liver tumors based on enhanced MR images, unenhanced MR images, and clinical data including text and laboratory test results. Using data from 1,210 patients with liver tumors ( = 31,608 images), we trained CNNs to get seven-way classifiers, binary classifiers, and three-way malignancy-classifiers (Model A-Model G). Models were validated in an external independent extended cohort of 201 patients ( = 6,816 images). The area under receiver operating characteristic (ROC) curve (AUC) were compared across different models. We also compared the sensitivity and specificity of models with the performance of three experienced radiologists. Deep learning achieves a performance on par with three experienced radiologists on classifying liver tumors in seven categories. Using only unenhanced images, CNN performs well in distinguishing malignant from benign liver tumors (AUC, 0.946; 95% CI 0.914-0.979 vs. 0.951; 0.919-0.982, = 0.664). New CNN combining unenhanced images with clinical data greatly improved the performance of classifying malignancies as hepatocellular carcinoma (AUC, 0.985; 95% CI 0.960-1.000), metastatic tumors (0.998; 0.989-1.000), and other primary malignancies (0.963; 0.896-1.000), and the agreement with pathology was 91.9%.These models mined diagnostic information in unenhanced images and clinical data by deep-neural-network, which were different to previous methods that utilized enhanced images. The sensitivity and specificity of almost every category in these models reached the same high level compared to three experienced radiologists. Trained with data in various acquisition conditions, DLS that integrated these models could be used as an accurate and time-saving assisted-diagnostic strategy for liver tumors in clinical settings, even in the absence of contrast agents. DLS therefore has the potential to avoid contrast-related side effects and reduce economic costs associated with current standard MRI inspection practices for liver tumor patients.
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http://dx.doi.org/10.3389/fonc.2020.00680DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7271965PMC
May 2020

Differences in the Magnitude of Motor Skill Acquisition and Interlimb Transfer between Left- and Right-Handed Subjects after Short-Term Unilateral Motor Skill Practice.

Tohoku J Exp Med 2020 05;251(1):31-37

Department of Medicine and Science in Sports and Exercise, Tohoku University Graduate School of Medicine.

Motor skill practice improves performance not only in the trained - but also in the untrained contralateral limb - a phenomenon called as interlimb transfer. Handedness affects motor skill acquisition and interlimb transfer, but it remains unknown whether handedness affects interlimb transfer when practicing with the dominant or non-dominant limb. We have hypothesized that interlimb transfer of skill acquisition differs between left- and right-handed participants, and that right- as compared with left-hand motor skill practice shows greater interlimb transfer, regardless of handedness. Strongly left-hand (n = 12, aged 27.3 ± 4.4 years; 3 female) and right-hand dominant (n = 12, 20.7 ± 3.8 years; 5 female) subjects with no history of neurological or orthopedic disorders performed the grooved pegboard test before and after 4 blocks of practice on the same apparatus. Subjects were timed on their speed of the task. Right-handed subjects failed to improve manual performance in their right hand after right- or left-hand motor practice. In contrast, they showed improvement on the left hand in each condition. These data suggest greater interlimb transfer after right-hand motor skill practice, but no interlimb transfer after left-hand practice. On the other hand, our results show consistent interlimb transfer effects in left-handed subjects, irrespective of whether the dominant left or the non-dominant right arm has been initially trained. In conclusion, our results add to the body of literature by detecting the differences in the magnitude of motor skill acquisition and interlimb transfer between left- and right-handed subjects after short-term unilateral motor skill practice.
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http://dx.doi.org/10.1620/tjem.251.31DOI Listing
May 2020

In Situ Pyrolysis Tracking and Real-Time Phase Evolution: From a Binary Zinc Cluster to Supercapacitive Porous Carbon.

Angew Chem Int Ed Engl 2020 Aug 20;59(32):13232-13237. Epub 2020 May 20.

Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry & Chemical Engineering, Hubei University, Wuhan, 430062, P. R. China.

The in situ tracking of the pyrolysis of a binary molecular cluster [Zn (μ -CH O) (L) ][ZnLCl ] is presented with one brucite disk and two mononuclear fragments (L=mmimp: 2-methoxy-6-((methylimino)-methyl)phenolate) to porous carbon using TG-MS from 30 to 900 °C. Following up the spilled gas product during the decomposed reaction of zinc cluster along the temperature rising, and in conjunction with XRD, SEM, BET and other materials characterization, where three key steps were observed: 1) cleavage of the bulky external ligand; 2) reduction of ZnO and 3) volatilization of Zn. The real-time-dependent phase-sequential evolution of the remaining products and the processing of pore forming template transformation are proposed simultaneously. The porous carbon structure featuring a uniform nano-sized pore distribution synthesized at 900 °C with the highest surface area of 1644 m  g and pore volume of 0.926 cm  g exhibits the best known capacitance of 662 F g at 0.5 A g .
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http://dx.doi.org/10.1002/anie.202004072DOI Listing
August 2020

Chromosome-scale assembly of the genome.

Hortic Res 2020 2;7:75. Epub 2020 May 2.

3Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at the College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou, 350002 China.

The mangrove (Rhizophoraceae) is an important coastal shelterbelt and landscape tree distributed in tropical and subtropical areas across East Asia and Southeast Asia. Herein, a chromosome-level reference genome of based on PacBio, Illumina, and Hi-C data is reported. The high-quality assembled genome size is 177.99 Mb, with a contig N50 value of 5.74 Mb. A large number of contracted gene families and a small number of expanded gene families, as well as a small number of repeated sequences, may account for the small genome. We found that experienced two whole-genome polyploidization events: one whole-genome duplication shared with other Rhizophoreae and one shared with most eudicots (γ event). We confidently annotated 19,138 protein-coding genes in and identified the MADS-box gene class and the gene class, which might be related to flowering and resistance to powdery mildew in and , respectively. The reference genome described here will be very useful for further molecular elucidation of various traits, the breeding of this coastal shelterbelt species, and evolutionary studies with related taxa.
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http://dx.doi.org/10.1038/s41438-020-0300-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7195387PMC
May 2020

[Effect of electroacupuncture of "Hegu" (LI4) and "Zusanli" (ST36) on intestinal sensitivity and motility in irritable bowel syndrome rats].

Zhen Ci Yan Jiu 2020 Apr;45(4):293-8

School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China.

Objective: To observe the effect of electroacupuncture (EA) of "Hegu"(LI4) and "Zusanli"(ST36)on changes of intestinal sensitivity and colonic motility and expression of colonic 5-hydroxytryptamine 3A receptor (5-HTR) in irritable bowel syndrome (IBS) rats, so as to reveal its mechanism underlying improvement of IBS.

Methods: A total of 40 neonatal Wistar rats were randomly and equally divided into normal control, model, LI4 and ST36 groups (=10). The IBS model was induced by mother-infant separation, acetic acid enema and colorectal distension (CRD). EA (2 Hz/100 Hz, a tolerable strength) was applied to bilateral LI4 and ST36 for 20 min, once every other day for 5 times. The Bristol stool form scale was used to assess the gastrointestinal function, and the latency and number of abdominal muscular contraction waves of abdominal withdrawal reflex (AWR) were used to evaluate the intestinal sensitivity and motility respectively. The immunoactivity of 5-HTR of the colon tissue was detected by immunohistochemistry.

Results: After modeling, the score of Bristol fecal form scale, number of muscular contraction waves and expression levels of colonic 5-HTR in the myometrium and mucosal layers were significantly increased (<0.01), and the latency of muscular initial contraction wave was obviously shortened in the model group relevant to the normal control group (<0.01). After the intervention, the increased Bristol fecal form score, number of muscular contraction waves and expression levels of 5-HTR in the myometrium and mucosal layers as well as the decreased latency of muscular contraction were reversed in both LI4 and ST36 groups (<0.01, <0.05). The effect of EA of ST36 was significantly superior to those of EA-LI4 in lowering Bristol fecal scale score and 5-HTR expression in the muscular layer (<0.01), but obviously inferior to those of EA-LI4 in increasing the latency of of muscular initial contraction wave and down-regulating muscular contraction waves and 5-HTR expression in the mucosal layer (<0.05, <0.01).

Conclusion: Both EA-LI4 and EA-ST36 can significantly improve the symptoms of abdominal pain and diarrhea, but EA-LI4 is better in suppressing intestinal high sensitivity, and EA-ST36 is better in promoting intestinal motility, suggesting a specificity of effect of acupoints of different meridians.
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http://dx.doi.org/10.13702/j.1000-0607.190743DOI Listing
April 2020

l-amino acid ligase: A promising alternative for the biosynthesis of l-dipeptides.

Enzyme Microb Technol 2020 May 26;136:109537. Epub 2020 Feb 26.

School of Biological Science, Jining Medical University, Jining, China. Electronic address:

Given their special action mechanisms and structural simplicity, -amino acid ligases (Lals) are considered to be desirable tools for the catalytic biosynthesis of dipeptides. Ywf E (BacD) was the first Lal identified and was shown to be involved in the biosynthesis of a potent antibacterial, bacilysin, since then, various novel Lals have been discovered. Each Lal has different substrate spectra and is capable of synthesizing different dipeptides. Owning to their great potentials for producing bioactive dipeptides of industrial importance, in this review, recent developments of Lals are discussed, including their structures, action mechanisms, applications and the advantages and disadvantages of different Lals. In addition, protein engineering of Lals to improve their substrate specificity and catalytic performance is also discussed.
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http://dx.doi.org/10.1016/j.enzmictec.2020.109537DOI Listing
May 2020

A binary sampling Res2net reconstruction network for single-pixel imaging.

Rev Sci Instrum 2020 Mar;91(3):033709

School of Information Engineering, Nanchang University, Nanchang 330031, China.

The traditional algorithm for compressive reconstruction has high computational complexity. In order to reduce the reconstruction time of compressive sensing, deep learning networks have proven to be an effective solution. In this paper, we have developed a single-pixel imaging system based on deep learning and designed the binary sampling Res2Net reconstruction network (Bsr2-Net) model suitable for binary matrix sampling. In the experiments, we compared the structural similarity, peak signal-to-noise ratio, and reconstruction time using different reconstruction methods. Experimental results show that the Bsr2-Net is superior to several deep learning networks recently reported and closes to the most advanced reconstruction algorithms.
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http://dx.doi.org/10.1063/1.5137817DOI Listing
March 2020
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