Publications by authors named "Yi Zhao"

1,501 Publications

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Annual trends and health risks of antibiotics and antibiotic resistance genes in a drinking water source in East China.

Sci Total Environ 2021 May 29;791:148152. Epub 2021 May 29.

State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China; Shanghai Environmental Protection Key Laboratory on Environmental Standard and Risk Management of Chemical Pollutants, East China University of Science & Technology, Shanghai 200237, China; National Engineering Laboratory for Industrial Wastewater Treatment, East China University of Science & Technology, Shanghai 200237, China. Electronic address:

The extensive pollution of antibiotics and antibiotic resistance genes (ARGs) in drinking water has aroused worldwide concern. Successive monitoring of these pollutants has noteworthy significance for drinking water safety. Accordingly, this study conducted successive monitoring of antibiotics and ARGs from 2015 to 2017 in a drinking water source in East China. The total antibiotic concentration ranged from 19.68 ng/L to 497.00 ng/L, and decreased slightly from 2015 to 2017. Eighteen out of forty-one ARG subtypes showing resistance to six antibiotic classes and one class I integrase gene intI1, were detected in the drinking water source at concentrations ranging from 6.5 × 10 copies/mL to 1.6 × 10 copies/mL. Importantly, the total ARG concentration increased on an annual basis from 2015 to 2017 with an average annual increment of 0.25 orders of magnitude, which was mainly attributed to the increase in specific ARG subtypes, such as sul1, sul2, sul3, tetA, qnrB, and ermB. Most ARGs was positively correlated with the intI1 genes (r = 0.47-0.55, P < 0.01). Furthermore, the variation of antibiotics and ARGs appeared to be related to the water indices, particularly of the values of COD, BOD NO-N (P < 0.05). This study provides basic data on antibiotic and ARG pollution in the studied drinking water source. Importantly, the findings expound that although the residual antibiotics in this drinking water source decreased slightly from 2015 to 2017, while its biological effect, the antibiotic resistance, increased annually, which give a warning of the antibiotic resistance pollution in the drinking water source.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148152DOI Listing
May 2021

Speciation, controlling steps and pathways of silver release from the sludge generated from coagulation of wastewater spiked with silver nanoparticles.

Chemosphere 2021 Jun 4;282:131093. Epub 2021 Jun 4.

Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Jinan, 250100, PR China.

Sludge generated in wastewater treatment facilities is an integral part for the introduction of silver nanoparticles (AgNPs) to the terrestrial environment, which would cause some adverse ecosystem responses. The understanding of silver release process from the sludge is important to evaluate their risks. In this study, the amount and speciation of the released silver were investigated by taking the sludge generated by wastewater coagulation with AgNPs added (denoted as sludge) an example, and kinetic analysis and density functional theory (DFT) calculations were first used to explore the controlling steps and pathways about the silver release. The results showed that sludge could release the dissolved silver and the colloidal silver. Beside Cl, Ca in the leaching solution could enhance the silver release of sludge, especially for the colloidal silver. The released colloidal silver restricted in size from 40 nm to 100 nm with irregular shape. Although the oxidative dissolution of Ag was the origin of the silver release pathways from the sludge, the silver diffusion was the controlling step due to the spontaneous binding between silver and the hydrolysates of polyaluminium chloride in sludge. However, Ca in the leaching solution could occupy the binding site of silver on sludge, which would increase the diffusion rate of silver over the oxidative rate of Ag. With this condition, the controlling step of silver release from sludge turned to the oxidative dissolution of Ag. Our findings are important to assess the fate of AgNPs in wastewater treatment as well as sludge applications.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131093DOI Listing
June 2021

Enhancement of carotenoid production and its regulation in edible mushroom Cordyceps militaris by abiotic stresses.

Enzyme Microb Technol 2021 Aug 24;148:109808. Epub 2021 Apr 24.

College of Food Science & Institute of Food Biotechnology, South China Agricultural University, Guangzhou, 510642, China; Research Center for Micro-Ecological Agent Engineering and Technology of Guangdong Province, Guangzhou, 510642, China. Electronic address:

Cordyceps militaris carotenoids are widely used as food additives, animal feed supplements, and so on. However, the biosynthetic pathway of carotenoids in C. militaris is still obscure. In this paper, changes of mycelial morphology and carotenoid accumulation of C. militaris were investigated under oxidative (KMnO) and osmotic stress (NaCl). Subsequently, qRT-PCR was employed to detect the expression levels of genes related to carotenogenesis to explore the mechanism of adaptation to abiotic stress. When the concentrations of KMnO and NaCl were respectively 0.4 g/L and 2 g/L, carotenoid accumulation reached a maximum of 6616.82 ± 666.43 μg/g and 6416.77 ± 537.02 μg/g. Under the oxidative stress condition of KMnO, the expressions of psy and hsp70 increased significantly compared with control. Besides, the genes fus3 and hog1 were significantly enriched in the MAPK signal pathway. Compared with the control group, there was no significant difference in expression of psy in the NaCl group. Moreover, the accumulation of triacylglycerols may contribute significantly to the increase in carotenoid accumulation. The increased accumulation of antioxidant carotenoids induced under environmental stress is to resist oxidative conditions. Fus3 and Hog1 signaling in the MAPK pathway was activated and subsequently take effects on the resistance of oxidative condition by regulating related metabolic processes. C. militaris resist the stress of high oxygen by producing a large amount of glycerol and carotenoids when this fungus is cultured in a saline environment for a long time.
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http://dx.doi.org/10.1016/j.enzmictec.2021.109808DOI Listing
August 2021

MicroRNA-532-5p-programmed cell death protein 4 (PDCD4) axis regulates angiotensin II-induced human umbilical vein endothelial cell apoptosis and proliferation.

Microvasc Res 2021 Jun 8:104195. Epub 2021 Jun 8.

Department of Neonatology, Kaifeng Maternity and Children Health Hospital, Kaifeng City, Henan Province 475002, PR China.

Background: This study was carried out to investigate the effect of microRNA miR-532-5p on the proliferation of hypertension endothelial cells.

Methods: Angiotensin II (Ang II)-treated human umbilical vein endothelial cells (HUVECs) and primary human aortic endothelial cells (HAECs) were used as cell models to imitate the pathological changes in endothelial cells under hypertensive conditions. The expression levels of miR-532-5p and programmed cell death protein 4 (PDCD4) were detected by Quantitative Real-time PCR (qRT-PCR). The effects of miR-532-5p and PDCD4 on the proliferation of HUVECs and HAECs treated with Ang II were detected by Methyl Thiazolyl Tetrazolium (MTT) assay. The effects of miR-532-5p and PDCD4 on the apoptosis and cell cycle of HUVECs and HAECs treated with Ang II were detected by flow cytometry. Western blot was used to detect the expression levels of PDCD4, apoptosis-related proteins and cycle-related proteins in HUVECs and HAECs treated with Ang II. Bioinformatics analysis and Luciferase gene reporter assay were used to assess the relationship between miR-532-5p and PDCD4.

Results: The expression levels of miR-532-5p were reduced, while the expression levels of PDCD4 were raised in Ang II-treated HUVECs and HAECs. MiR-532-5p mimic and si-PDCD4 restrained the apoptosis, promoted the proliferation of Ang II-treated HUVECs and HAECs and caused S-phase arrest of cells. PDCD4 was identified as a potential target for miR-532-5p. Knockdown of PDCD4 significantly affected apoptosis and proliferation of Ang II-treated HUVECs. MiR-532-5p regulates apoptosis and proliferation of Ang II-induced HUVECs and HAECs. In addition, overexpression of PDCD4 attenuated the effect of miR-532-5p on the proliferation of Ang II-treated HUVECs and HAECs.

Conclusion: MiR-532-5p inhibited the expression of PDCD4, thereby inhibiting apoptosis and promoting proliferation of Ang II-treated HUVECs and HAECs.
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http://dx.doi.org/10.1016/j.mvr.2021.104195DOI Listing
June 2021

The mRNA-Binding Protein HuR Is a Kinetically-Privileged Electrophile Sensor.

Helv Chim Acta 2020 May 12;103(5). Epub 2020 Apr 12.

Institute of Chemical Sciences & Engineering (ISIC), Swiss Federal Institute of Technology Lausanne (EPFL), CH-1015 Lausanne.

The key mRNA-binding proteins HuR and AUF1 are reported stress sensors in mammals. Intrigued by recent reports of sensitivity of these proteins to the electrophilic lipid prostaglandin A2 and other redox signals, we here examined their sensing abilities to a prototypical redox-linked lipid-derived electrophile, 4-hydroxynonenal (HNE). Leveraging our T-REX electrophile delivery platform, we found that only HuR, and not AUF1, is a kinetically-privileged sensor of HNE in HEK293T cells, and sensing functions through a specific cysteine, C13. Cells depleted of HuR, upon treatment with HNE, manifest unique alterations in cell viability and Nrf2-transcription-factor-driven antioxidant response (AR), which our recent work shows is regulated by HuR at the Nrf2-mRNA level. Mutagenesis studies showed that C13-specific sensing alone is not sufficient to explain HuR-dependent stress responsivities, further highlighting a complex context-dependent layer of Nrf2/AR regulation through HuR.
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http://dx.doi.org/10.1002/hlca.202000041DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188987PMC
May 2020

DeepOmix: A scalable and interpretable multi-omics deep learning framework and application in cancer survival analysis.

Comput Struct Biotechnol J 2021 1;19:2719-2725. Epub 2021 May 1.

Key Laboratory of Intelligent Information Processing, Advanced Computer Research Center, Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100190, China.

Integrative analysis of multi-omics data can elucidate valuable insights into complex molecular mechanisms for various diseases. However, due to their different modalities and high dimension, utilizing and integrating different types of omics data suffers from great challenges. There is an urgent need to develop a powerful method to improve survival prediction and detect functional gene modules from multi-omics data. To deal with these problems, we present DeepOmix (a scalable and interpretable multi-Omics Deep learning framework and application in cancer survival analysis), a flexible, scalable, and interpretable method for extracting relationships between the clinical survival time and multi-omics data based on a deep learning framework. DeepOmix enables the non-linear combination of variables from different omics datasets and incorporates prior biological information defined by users (such as signaling pathways and tissue networks). Benchmark experiments demonstrate that DeepOmix outperforms the other five cutting-edge prediction methods. Besides, Lower Grade Glioma (LGG) is taken as the case study to perform the prognosis prediction and illustrate the functional module nodes which are associated with the prognostic result in the prediction model.
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http://dx.doi.org/10.1016/j.csbj.2021.04.067DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8131983PMC
May 2021

Expression of lncRNA NEAT1 in peripheral blood mononuclear cells of patients with systemic lupus erythematosus and its correlation with Th1/Th2 balance.

Int J Clin Exp Pathol 2021 15;14(5):646-652. Epub 2021 May 15.

Department of Rheumatology and Immunology, West China Hospital, Sichuan University Chengdu 610041, Sichuan, China.

Objective: This study explored and analyzed the expression of LncRNA NEAT1 in peripheral blood mononuclear cells (PBMCs) of patients with systemic lupus erythematosus (SLE) and its correlation with Th1/Th2 balance.

Methods: We chose 97 SLE patients admitted in our hospital from Jun. 2016 to Feb. 2019 as SLE group, and randomly selected 50 healthy volunteers that underwent physical examination in our hospital during the same period as control group. We detected the expression of LncRNA NEAT1 in PBMCs of the two groups of subjects by qRT-PCR, the degree of Th1 and Th2 cells in both groups by flow cytometry, and the expression of TFN-γ and IL-4 in both groups by ELISA.

Results: The relative expression of LncRNA NEAT1 in PBMCs of SLE group was higher than that of control group (). The proportion of Th1 and the ratio of Th1/Th2 cells in PBMCs were markedly lower in the SLE group than the control group (), while the proportion of Th2 was higher in the SLE group than the control group (). IFN-γ level in SLE group was much lower than the control group (), while IL-4 level was evidently higher in the SLE group than in controls (). The expression of LncRNA NEAT1 in PBMCs of SLE group was notably negatively correlated with Th1 proportion and Th1/Th2 ratio (), while positively correlated with Th2 proportion ().

Conclusion: LncRNA NEAT1 in PBMCs of SLE patients is abnormally highly expressed, and this expression is negatively correlated with Th1/Th2 balance. These two factors may interact and jointly affect the occurrence and progression of SLE.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167491PMC
May 2021

Case Report: Anti-N-Methyl-D-Aspartate Receptor Encephalitis in an Elderly Patient With Primary Sjögren's Syndrome.

Front Neurol 2021 20;12:656024. Epub 2021 May 20.

Department of Rheumatology and Allergy, Xuanwu Hospital, Capital Medical University, Beijing, China.

Neurological manifestations of primary Sjögren's syndrome (SS) are diverse involving the peripheral and central nervous system. Anti-N-methyl-D-aspartate receptor (NMDAR) encephalitis, as the most prevalent autoimmune encephalitis, was rarely reported to be complicated with primary SS. Herein, we present an elderly patient with a 15-year history of primary SS presenting with progressive cognitive dysfunction due to anti-NMDAR encephalitis that was once misdiagnosed as primary degenerative dementia. Early recognition of anti-NMDAR encephalitis and initiation of treatment with steroids and immunosuppressant gained a favorable outcome. Our findings enhance the awareness that autoimmune encephalitis should be taken into account in the patients with primary SS presenting with progressive cognitive impairment.
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http://dx.doi.org/10.3389/fneur.2021.656024DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8172793PMC
May 2021

The long-term outcomes of posterior nasal neurectomy with or without pharyngeal neurectomy in patients with allergic rhinitis: a randomized controlled trial.

Braz J Otorhinolaryngol 2021 May 29. Epub 2021 May 29.

First Affiliated Hospital of Anhui Medical University, Department of Otorhinolaryngology Head and Neck Surgery, Hefei, Anhui, China. Electronic address:

Introduction: Allergic rhinitis is a form of IgE mediated inflammation of the nasal mucosa in response to specific allergens, resulting in typical symptoms.

Objectives: This study was designed with the primary goal of comparing the clinical efficacy of posterior nasal neurectomy with or without pharyngeal neurectomy for the treatment of moderate-to-severe perennial allergic rhinitis. Secondary study aims included a comparison of the severity of comorbidities, including chronic cough and asthma, between patients in these two surgical treatment groups.

Methods: A total of 52 patients were enrolled in this randomized controlled trial and were assigned to either the control group (posterior nasal neurectomy) or the experimental group (posterior nasal neurectomy + pharyngeal neurectomy). The visual analog scale and rhinoconjunctivitis quality of life questionnaire were used to compare the differences in patient symptoms between baseline and 6-, 12-, and 24-months post-treatment. In addition, patient cough and asthma symptoms were monitored during follow-up via visual analog scale and asthma control test respectively.

Results: No significant differences in preoperative scores were evident between groups (p > 0.05). At 6-months post-treatment, there were significant differences in visual analog scale, rhinoconjunctivitis quality of life questionnaire, and asthma control test scores relative to baseline values in experimental group and control group patients (p < 0.05), and this remained true upon 12- and 24-month follow-up. No significant differences in visual analog scale, rhinoconjunctivitis quality of life questionnaire, or asthma control test scores were observed between the two treatment groups at any postoperative follow-up time point (p > 0.05), while coughing severity was found to be significantly reduced in the experimental group relative to the control group (p < 0.05).

Conclusion: posterior nasal neurectomy can be safely implemented with or without pharyngeal neurectomy in order to effectively treat allergic rhinitis. Combined posterior nasal neurectomy and pharyngeal neurectomy treatment may offer greater value than posterior nasal neurectomy alone for the treatment of allergic rhinitis patients with chronic cough.
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http://dx.doi.org/10.1016/j.bjorl.2021.05.006DOI Listing
May 2021

DTI Atlases Evaluations.

Neuroinformatics 2021 Jun 4. Epub 2021 Jun 4.

School of Marine Science and Technology, Northwestern Polytechnical University, Xi'an, 710072, China.

The cerebral atlas of diffusion tensor magnetic resonance image (DT-MRI, shorted as DTI) is one of the key issues in neuroimaging research. It is crucial for comparisons of neuronal structural integrity and connectivity across populations. Usually, the atlas is constructed by iteratively averaging the registered individual image. In tradition, the fuzzy group average image is easily generated in the initial stage, which is harmful to providing clear guidance for subsequent registration, to the performance of the final atlas. To solve this problem, an improved unbiased DTI atlas construction algorithm based on adaptive weights is proposed in this paper. The adaptive weighted strategy based on diffeomorphic deformable tensor registration is introduced. At the same time, the distance measure for tensors is used as a constraint condition, which ensures the unbiasedness of the atlas. Then, using 77 DTIs from the dataset in http://www.brain-development.org , three study-specific atlases, i.e. the constructed atlases of the proposed algorithm and two open-sourced algorithms (DTIAtlasBuilder and DTI-TK), are compared with two standardized atlases (IIT v. 4.1 and NTU-DSI-122-DTI). The performances of the atlases were evaluated in spatial normalization way with six region-based criteria (including Euclidean distances between diffusion tensors, Euclidean distances of the deviatoric tensors, standard deviation, overlaps of eigenvalue-eigenvector, cross-correlations and three sets angles of eigenvalue-eigenvector pairs between diffusion tensors) and three fiber-based criteria (including distances between fiber bundles, angles between fiber bundles and fiber property profile-based criteria). The experimental results showed that the overall performances of the study-specific atlases are better than those of the standardized atlases for specific datasets, and the comprehensive performance of the improved algorithm proposed in this paper is the best.
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http://dx.doi.org/10.1007/s12021-021-09521-yDOI Listing
June 2021

Insights into influences of sucrose amendment on nitrification and denitrification in sewage sludge composting.

Chemosphere 2021 Aug 15;276:130245. Epub 2021 Mar 15.

Institute of Microbiology, Heilongjiang Academy of Sciences, 150010, Harbin, China.

Sucrose amendment could promote ammonia assimilation and reduce nitrogen loss in sewage sludge (SS) composting, but the effects of sucrose amendment on nitrification and denitrification are still unknown that were firstly researched in present paper. Result showed that sucrose amendment reduced 33.0% of NO emission by changing the physicochemical indexes, nitrogen forms, related bacteria and functional genes. In the sucrose treatment, the higher nitrifying bacteria community, amoA and nxrA genes abundance were, the lower hao, narG、nirS、nirK and norB genes abundance were. Based on the correlation analysis, the number of nitrifying bacteria was significantly positively correlated with NO and nxrA abundance, indicating that sucrose amendment promoted the growth of nitrifying bacteria, the contents of NO and the activity of nitrite oxidation. Moreover, contents of NO were positively correlated with NO emission, narG, nirS and norB abundance, indicating that denitrification was the main path of NO generated.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130245DOI Listing
August 2021

KOBAS-i: intelligent prioritization and exploratory visualization of biological functions for gene enrichment analysis.

Nucleic Acids Res 2021 Jun 4. Epub 2021 Jun 4.

Center for Bioinformatics, State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China.

Gene set enrichment (GSE) analysis plays an essential role in extracting biological insight from genome-scale experiments. ORA (overrepresentation analysis), FCS (functional class scoring), and PT (pathway topology) approaches are three generations of GSE methods along the timeline of development. Previous versions of KOBAS provided services based on just the ORA method. Here we presented version 3.0 of KOBAS, which is named KOBAS-i (short for KOBAS intelligent version). It introduced a novel machine learning-based method we published earlier, CGPS, which incorporates seven FCS tools and two PT tools into a single ensemble score and intelligently prioritizes the relevant biological pathways. In addition, KOBAS has expanded the downstream exploratory visualization for selecting and understanding the enriched results. The tool constructs a novel view of cirFunMap, which presents different enriched terms and their correlations in a landscape. Finally, based on the previous version's framework, KOBAS increased the number of supported species from 1327 to 5944. For an easier local run, it also provides a prebuilt Docker image that requires no installation, as a supplementary to the source code version. KOBAS can be freely accessed at http://kobas.cbi.pku.edu.cn, and a mirror site is available at http://bioinfo.org/kobas.
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http://dx.doi.org/10.1093/nar/gkab447DOI Listing
June 2021

FBXO15 plays a critical suppressive functional role in regulation of breast cancer progression.

Signal Transduct Target Ther 2021 Jun 4;6(1):211. Epub 2021 Jun 4.

Department of Life Science, Research Institute for Natural Sciences, Hanyang University, Seoul, South Korea.

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http://dx.doi.org/10.1038/s41392-021-00605-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175582PMC
June 2021

Glucose and Triphenylphosphonium Co-Modified Redox-Sensitive Liposomes to Synergistically Treat Glioma with Doxorubicin and Lonidamine.

ACS Appl Mater Interfaces 2021 Jun 1. Epub 2021 Jun 1.

Key Laboratory of Drug-Targeting and Drug Delivery System of the Education Ministry and Sichuan Province, Sichuan Engineering Laboratory for Plant-Sourced Drug and Sichuan Research Center for Drug Precision Industrial Technology, West China School of Pharmacy, Sichuan University, Chengdu 610041, China.

Glioma is one of the most lethal and complex tumors, and thus, an effective drug delivery system must selectively target the tumor sites and release its cargos in a controlled manner. For the first time, we combined chemotherapeutic agent doxorubicin (DOX) and chemosensitizer lonidamine (LND) to synergistically treat glioma. We also designed and prepared multitargeted redox-sensitive liposomes (Lip-SPG) co-modified with glucose and triphenylphosphonium (TPP) to effectively deliver DOX and LND for anti-glioma therapy. The anti-glioma evaluation shows that DOX and LND have a synergistic effect and Lip-SPG could further enhance their cooperation. , Lip-SPG could increase the cellular uptake and mitochondrial uptake on bEnd.3 cells and C6 cells with multitargeting ability on the brain, tumor, and mitochondria mediated by glucose and TPP. Lip-SPG can also escape from lysosomes before entering the mitochondria. The anti-glioma efficacy shows that Lip-SPG can inhibit tumor cell proliferation and induce apoptosis. In addition, Lip-SPG have a remarkable interference to mitochondria, such as reducing intracellular ATP production, inducing ROS generation, and promoting mitochondrial membrane potential depolarization. Furthermore, , the introduction of PEGylation via glutathione-sensitive disulfide bonds endows Lip-SPG with favorable pharmacokinetic properties, brain targeting ability, low toxicity to normal tissues, and great anti-glioma efficacy with the survival time extended from 19 to 39 days. In conclusion, Lip-SPG are an effective delivery system for synergistically treating glioma with DOX and LND.
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http://dx.doi.org/10.1021/acsami.1c02404DOI Listing
June 2021

Development of unenhanced CT-based imaging signature for BAP1 mutation status prediction in malignant pleural mesothelioma: Consideration of 2D and 3D segmentation.

Lung Cancer 2021 Jul 29;157:30-39. Epub 2021 Apr 29.

Department of Medical Imaging, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650032, China. Electronic address:

Objectives: We aimed to explore the feasibility of 2D and 3D radiomics signature based on the unenhanced computed tomography (CT) images to predict BRCA1-associated protein 1 (BAP1) gene mutation status for malignant pleural mesothelioma (MPM) patients.

Materials And Methods: 74 patients with MPM were retrospectively enrolled (22 mutant BAP1, 52 wild-type BAP1 demonstrated by Sanger sequencing). The radiomic features were extracted respectively from the 2D and 3D segmentation of unenhanced pre-treatment CT images, and the dataset was randomly divided into training (n = 51) and test (n = 23) sets for radiomics model development and internal validation. The synthetic minority over-sampling technique (SMOTE) was used for data balancing in the training set. 2D or 3D features were sequentially selected by ICC > 0.8, correlation analysis (cut-value 0.7), univariate analysis or univariate logistic regression (LR), which were involved into multivariate LR for LR model construction. Following the comparison of the 2D and 3D models by the ROC analysis and Delong test for AUC, the calibration and clinical utility of 2D and 3D models were evaluated.

Results: 3D radiomic features showed better ICCs compared with 2D in both intra- (P < 0.001) and inter-observer (P <  0.001) analysis. 3D radiomic model based on selected features developed from a balanced training dataset presented a favorable predictive performance with AUC of 0.786 and 0.768 in the training and test sets, respectively. The predictive performance of 3D model was superior to 2D model (1 feature) both in the training (AUC 0.786 vs. 0.683, P = 0.036) and the test (AUC 0.768 vs.0.652, P = 0.441) set. The calibration curve and decision curves also indicate a better BAP1 prediction performance and clinical benefit for 3D model than that of 2D model.

Conclusion: The developed unenhanced CT-based 3D radiomics signature is potential as a noninvasive marker for predicting BAP1 mutation status.
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http://dx.doi.org/10.1016/j.lungcan.2021.04.023DOI Listing
July 2021

ncFANs v2.0: an integrative platform for functional annotation of non-coding RNAs.

Nucleic Acids Res 2021 May 29. Epub 2021 May 29.

Pervasive Computing Research Center, Institute of Computing Technology, Chinese Academy of Sciences, Beijing, 100190, China.

Increasing evidence proves the essential regulatory roles of non-coding RNAs (ncRNAs) in biological processes. However, characterizing the specific functions of ncRNAs remains a challenging task, owing to the intensive consumption of the experimental approaches. Here, we present an online platform ncFANs v2.0 that is a significantly enhanced version of our previous ncFANs to provide multiple computational methods for ncRNA functional annotation. Specifically, ncFANs v2.0 was updated to embed three functional modules, including ncFANs-NET, ncFANs-eLnc and ncFANs-CHIP. ncFANs-NET is a new module designed for data-free functional annotation based on four kinds of pre-built networks, including the co-expression network, co-methylation network, long non-coding RNA (lncRNA)-centric regulatory network and random forest-based network. ncFANs-eLnc enables the one-stop identification of enhancer-derived lncRNAs from the de novo assembled transcriptome based on the user-defined or our pre-annotated enhancers. Moreover, ncFANs-CHIP inherits the original functions for microarray data-based functional annotation and supports more chip types. We believe that our ncFANs v2.0 carries sufficient convenience and practicability for biological researchers and facilitates unraveling the regulatory mechanisms of ncRNAs. The ncFANs v2.0 server is freely available at http://bioinfo.org/ncfans or http://ncfans.gene.ac.
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http://dx.doi.org/10.1093/nar/gkab435DOI Listing
May 2021

Basophils as a potential marker of lupus nephritis by flow cytometry.

Future Sci OA 2021 Feb 16;7(5):FSO690. Epub 2021 Feb 16.

Department of Rheumatology & Immunology, West China Hospital, Sichuan University, Chengdu, China.

Objective: To establish a convenient and simple flow cytometry immunophenotyping panel to explore immune cellular alterations and potential cellular biomarkers in systemic lupus erythematosus.

Materials And Methods: This is a cross-sectional, case-control study including 60 patients with systemic lupus erythematosus and 20 sex- and age-matched healthy controls. A 14-color immunophenotyping panel was applied to detect proportions of circulating immune mononuclear cells, and comparisons between patients and healthy controls, and subgroups of patients, were performed. Correlations between cellular proportions and other parameters were investigated.

Results: After multivariate analysis, significantly decreased proportions of CD4CD8 T cells, natural killer cells and innate lymphoid cells were observed in patients compared with healthy controls. The proportions of basophils were decreased significantly in patients with lupus nephritis (LN) compared with those in patients without LN.

Conclusion: In the present study, we found that basophil proportions may be a biomarker of LN.
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http://dx.doi.org/10.2144/fsoa-2020-0212DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147755PMC
February 2021

STEP® vectors for rapid generation of stable transfected CHO cell pools and clones with high expression levels and product quality homogeneity of difficult-to-express proteins.

Protein Expr Purif 2021 May 24:105920. Epub 2021 May 24.

Batavia Biosciences Inc., 300 Trade Center Suite 6650, Woburn, MA, USA. Electronic address:

Many proteins produced in CHO cells need evaluation for their clinical and commercial potential. Traditional methods based on stable clone generation are slow and unsuitable for screening larger numbers of proteins, while transient expression technologies are fast but unpredictable regarding product quality and lacking an optional path to subcloning. The STEP® vector technology introduced here combines the best properties of both methods. STEP® vectors contain a strong transcriptional cassette driving expression of a bicistronic mRNA. The gene-of-interest (GOI) is cloned upstream of a functionally impaired zeocin resistance gene (FI-Zeo) whose translation is coupled to that of the GOI through an IRES. Stable transfected cells surviving zeocin selection produce high levels of FI-Zeo and thus, high levels of the GOI-encoded protein. By using different spacers, the translational coupling efficiency and selection strength can be controlled allowing maximization of expression of any GOI. Production of laronidase and factor VII (FVII) is presented as examples of unrelated, difficult-to-express (DTE) proteins. First step is rapid generation of transfected pools with the STEP® vectors. All high expressing surviving pools showed high product quality homogeneity as did monoclonal cell lines obtained from the top pools. Up to 500 μg/mL laronidase was obtained with virtually identical glycosylation profile as reference product. For FVII, cell specific productivity of 0.45 pg/cell/day with 50IU/μg protein matched highest reported levels of reference product even before process development. Taken together, STEP® vector technology is ideally suited for rapid, small to large-scale production of DTE proteins compared to traditional methods.
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http://dx.doi.org/10.1016/j.pep.2021.105920DOI Listing
May 2021

A nomogram to predict the risk of lupus enteritis in systemic lupus erythematosus patients with gastroinctestinal involvement.

EClinicalMedicine 2021 Jun 24;36:100900. Epub 2021 May 24.

Department of Rheumatology and Immunology, West China Hospital, Sichuan University, Chengdu, China.

Background: Lupus enteritis (LE), a main cause of acute abdominal pain in systemic lupus erythematosus (SLE) patients, is a serious and potentially fatal complication. This study aimed to identify clinical serological indicators to establish a nomogram to assess LE in SLE patients with gastrointestinal manifestations.

Methods: The clinical and laboratory data of SLE patients with gastrointestinal manifestations that were hospitalized in the West China Hospital from January 2010 to January 2020 were retrospectively analyzed. The least absolute shrinkage and selection operator logistic regression model was used to select potentially relevant features. Subsequently, a nomogram was developed using multivariable logistic analysis. The performance of the nomogram was evaluated using a receiver operating characteristic curve, a calibration curve, and decision curve analysis (DCA).

Findings: We included a total of 8,505 SLE patients, of which 251 had experienced gastrointestinal manifestations. The patients were randomly divided into training ( = 176) and validation ( = 75) groups. The LRA (LE Risk Assessment) model consisted of 11 significantly associated variables, which included complement 4, antineutrophil cytoplasmic antibody, albumin, anion gap, age, d-dimer, platelet, serum chlorine, anti-Sjögren's-syndrome-related antigen A, anti-ribosomal P protein, and anti-ribonucleoprotein. In the training and validation cohorts, the areas under the curve were 0.919 (95% confidence interval [CI]: 0.876-0.962) and 0.870 (95% CI: 0.775-0.964), respectively. The nomogram demonstrated excellent performance in the calibration curve and DCA.

Interpretation: The LRA model exhibits good predictive ability in assessing LE risk in SLE patients with gastrointestinal manifestations.
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http://dx.doi.org/10.1016/j.eclinm.2021.100900DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144679PMC
June 2021

Neutrophil-to-Lymphocyte Ratio on Admission is an Independent Risk Factor for the Severity of Neurological Impairment at Disease Onset in Patients with a First Episode of Neuromyelitis Optica Spectrum Disorder.

Neuropsychiatr Dis Treat 2021 18;17:1493-1503. Epub 2021 May 18.

Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, People's Republic of China.

Purpose: To investigate the relationship between the neutrophil-to-lymphocyte ratio (NLR) and the severity of neurological impairment at disease onset in patients with a first episode of neuromyelitis optica spectrum disorder (NMOSD).

Patients And Methods: This retrospective study included 259 patients with newly diagnosed NMOSD who were hospitalized at our institution between January 2013 and January 2020 (NMOSD group) and 169 healthy control subjects who underwent a physical examination at our hospital during the same period (control group). The clinical data collected included general information, past medical history, biochemical test results, imaging findings, NLR, AQP-4 antibody status, and initial Expanded Disability Status Scale score. A logistic regression model was used to analyze NLR as an independent risk factor for the severity of neurological impairment at disease onset in the NMOSD group. Receiver-operating characteristic curve analysis was used to evaluate the ability of the NLR to predict the severity of neurological impairment at disease onset in the NMOSD group and to determine its critical value.

Results: The NLR was significantly higher in the NMOSD group than in the control group (<0.001). In the NMOSD group, neurological impairment at disease onset was more severe in those with a high NLR than in those with a low NLR (<0.001). At onset of disease, patients with severe neurological impairment had a more significant increase in NLR than those with mild-to-moderate neurological impairment (<0.001). Both univariate (OR 1.180, 95% CI 1.046-1.331, =0.007) and multivariate (OR 1.146, 95% CI 1.003-1.308, =0.044) logistic regression analyses showed that the NLR was positively correlated with the severity of neurological impairment at onset of disease in the NMOSD group. The area under the receiver-operating characteristic curve was 0.687.

Conclusion: The NLR is an independent risk factor for the severity of neurological impairment at disease onset in patients with a first episode of NMOSD.
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http://dx.doi.org/10.2147/NDT.S311942DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8140946PMC
May 2021

Systematic review and practical guidance on the use of topical calcipotriol and topical calcipotriol with betamethasone dipropionate as long-term therapy for mild-to-moderate plaque psoriasis.

J Dermatol 2021 May 25. Epub 2021 May 25.

King Abdulaziz University, Jeddah, Saudi Arabia.

While many patients with psoriasis are candidates for topical agents, long-term treatment effects are unclear. This systematic review evaluated global findings from clinical trials and real-world studies of topical calcipotriol and the two-compound formulation of calcipotriol and betamethasone dipropionate for mild-to-moderate plaque psoriasis (including scalp psoriasis). PubMed, Embase and MEDLINE were searched for relevant English-language publications along with Chinese, Japanese, Korean and Latin American publication databases. Identified articles were screened by title and abstract against predefined inclusion/exclusion criteria. A narrative synthesis of key efficacy and safety findings from the full papers of selected publications was developed. Thirty-seven relevant papers were identified (25 English, 11 Chinese and one Japanese-language study) including 28 randomized controlled trials. While there was significant heterogeneity in study length, treatment intensity and clinical measures, following a critical review of the published data combined with expert opinion, the following clinical practice recommendations were agreed in order to assist healthcare providers: in adults, long-term treatment with calcipotriol/betamethasone dipropionate is well tolerated and efficacious for up to 1 year on an 'as needed' basis, and for up to 16 weeks on a fixed-treatment regimen. Calcipotriol is also well tolerated and efficacious when used long term (up to 52 weeks) 'as needed' and for up to 20 weeks on a fixed-treatment regimen. Used on an 'as needed' basis for up to 1 year, the safety and efficacy profile of fixed-dose combination calcipotriol/betamethasone dipropionate is more favorable than calcipotriol alone; regular consultation between patients and their dermatologist/primary care physician is required to review psoriasis symptoms and adjust treatment accordingly; a specific treatment goal should be agreed on initiation of topical agent(s) to determine when long-term treatment can begin or if a regimen change is warranted; and application frequency during the continued treatment phase should consider the patients' treatment expectations and goals.
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http://dx.doi.org/10.1111/1346-8138.15806DOI Listing
May 2021

IL-21 Is an Accomplice of PD-L1 in the Induction of PD-1-Dependent Treg Generation in Head and Neck Cancer.

Front Oncol 2021 5;11:648293. Epub 2021 May 5.

Department of Otorhinolaryngology Head and Neck Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Regulatory T cells (Tregs) are immunosuppressive cells involved in antitumor immunity. However, the regulation of Treg generation by inflammation in the tumor microenvironment has not been carefully investigated. Here, we demonstrated that IL-21-polarized inflammation was enriched in the tumor microenvironment in head and neck squamous cell carcinoma (HNSCC) and that IL-21 could promote PD-L1-induced Treg generation in a PD-1-dependent manner. Moreover, generated Tregs showed a greater ability to suppress the proliferation of tumor-associated antigen (TAA)-specific T cells than naturally occurring Tregs. Importantly, an anti-PD-1 antibody could inhibit only Treg expansion induced by clinical tumor explants with high expression of IL-21/PD-L1. In addition, neutralizing IL-21 could enhance the anti-PD-1 antibody-mediated inhibitory effect on Treg expansion. Furthermore, simultaneous high expression of IL-21 and PD-L1 was associated with more Treg infiltrates and predicted reduced overall and disease-free survival in patients with HNSCC. These findings indicate that IL-21 in the tumor microenvironment may promote PD-L1-induced, Treg-mediated immune escape in a PD-1-dependent manner and that an IL-21 neutralization strategy may enhance PD-1 blockade-based antitumor immunotherapy by targeting Treg-mediated immune evasion in patients with high expression of IL-21 and PD-L1.
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http://dx.doi.org/10.3389/fonc.2021.648293DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8131831PMC
May 2021

Transcriptome landscape of double negative T cells by single-cell RNA sequencing.

J Autoimmun 2021 Jul 19;121:102653. Epub 2021 May 19.

General Surgery Department, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China; National Clinical Research Center for Digestive Diseases, Beijing, 100050, China; Beijing Key Laboratory of Tolerance Induction and Organ Protection in Transplantation, Beijing, 100050, China; Immunology Research Center for Oral and Systemic Health, Beijing Friendship Hospital, Capital Medical University, China; Beijing Clinical Research Institute, Beijing, 100050, China. Electronic address:

CD4 and CD8 coreceptor double negative TCRαβ T (DNT) cells are increasingly being recognized for their critical and diverse roles in the immune system. However, their molecular and functional signatures remain poorly understood and controversial. Moreover, the majority of studies are descriptive because of the relative low frequency of cells and non-standardized definition of this lineage. In this study, we performed single-cell RNA sequencing on 28,835 single immune cells isolated from mixed splenocytes of male C57BL/6 mice using strict fluorescence-activated cell sorting. The data was replicated in a subsequent study. Our analysis revealed five transcriptionally distinct naïve DNT cell clusters, which expressed unique sets of genes and primarily performed T helper, cytotoxic and innate immune functions. Anti-CD3/CD28 activation enhanced their T helper and cytotoxic functions. Moreover, in comparison with CD4, CD8 T cells and NK cells, Ikzf2 was highly expressed by both naïve and activated cytotoxic DNT cells. In conclusion, we provide a map of the heterogeneity in naïve and active DNT cells, addresses the controversy about DNT cells, and provides potential transcription signatures of DNT cells. The landscape approach herein will eventually become more feasible through newer high throughput methods and will enable clustering data to be fed into a systems analysis approach. Thus the approach should become the "backdrop" of similar studies in the myriad murine models of autoimmunity, potentially highlighting the importance of DNT cells and other minor lineage of cells in immune homeostasis. The clear characterization of functional DNT subsets into helper DNT, cytotoxic DNT and innate DNT will help to better understand the intrinsic roles of different functional DNT subsets in the development and progression of autoimmune diseases and transplant rejection, and thereby may facilitate diagnosis and therapy.
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http://dx.doi.org/10.1016/j.jaut.2021.102653DOI Listing
July 2021

Diagnosis and Management of Headaches in the Emergency Department (ED) in Adults and Children.

Neurol India 2021 Mar-Apr;69(Supplement):S173-S182

SGH Department of Pharmacy, NUS Dept of Pharmacy, Singapore.

Background: Headache accounts for a significant number of cases presenting to the Emergency Department (ED) and has a high societal cost, contributed by recurrent ED and clinic visits, and unnecessary diagnostic tests.

Objective: This review article covers the important clinical tools needed to evaluate headaches in both adults and children in the ED.

Methods: Medline/PubMed was searched using the keywords "Emergency department", "headache", "adult", "pediatric", "clinical assessment", "diagnosis" and "treatment", in the title or abstract. The search covers the period from 1 January 1990 to 31 December 2019.

Results: The articles selected were based on their relevancy to the objective of this review article. Additional relevant publications were identified from article references lists.

Conclusion: The emergency physician plays a key role in differentiating between primary and secondary headaches. Within the limited ED resources, appropriate diagnostic testing should be used to identify the life-threatening headaches. This will ensure patients are given the appropriate evidence-based pharmacological therapy and holistic management.
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http://dx.doi.org/10.4103/0028-3886.315977DOI Listing
May 2021

Clinical characteristics and outcome of tumor-associated acute pancreatitis: a single-center cohort study.

Ann Transl Med 2021 Apr;9(8):639

Department of Gastroenterology, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.

Background: What features should alert clinicians to suspect underlying tumors in patients with acute pancreatitis (AP) was largely unknown. This study aimed to assess the clinical characteristics and outcome in patients with tumor-associated AP.

Methods: Patients who presented with AP and were diagnosed with tumor after admission were included according to the inclusion and exclusion criteria and followed up by hospital notes, telephone, WeChat and/or e-mail. The clinical characteristics and outcome were analyzed with multivariable logistic regression and were compared with AP patients without tumor.

Results: Out of a cohort of 1,792 AP patients we identified 103 who had a neoplastic etiology. The 103 patients had a median age of 57 (range, 13-81) and 65 were males. AP was mild in 92 patients, moderately severe in 7 and severe in 4. The three most common tumors included pancreatic cancer (PC) (40), periampullary carcinoma (PAC) (25), and neoplastic pancreatic cysts (NPC) (22). The following ranked features were predictive of a tumor etiology: dilation of main pancreatic duct (MPD) (OR 417.83, 95% CI: 80.40-2,171.42), vascular invasion (OR 82.04, 95% CI: 6.05-1,113.14), mild AP (8.29, 95% CI: 1.98-34.73), and anemia (OR 5.73, 95% CI: 2.02-16.26). The median survival period of AP patients with PC, PAC, and NPC was 10.0 (7.0-23.5), 21.0 (5.0-37.0), and 35.0 (30.0-96.0) months, respectively.

Conclusions: Mild AP patients with dilation of MPD, vascular invasion, and anemia were more frequently suggested a tumor etiology. Thus, clinical vigilance is needed for timely detection of tumor-associated pancreatitis with these characteristics.
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http://dx.doi.org/10.21037/atm-20-7196DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106109PMC
April 2021

Circulating Exosomal MiR-107 Restrains Tumorigenesis in Diffuse Large B-Cell Lymphoma by Targeting 14-3-3η.

Front Cell Dev Biol 2021 27;9:667800. Epub 2021 Apr 27.

Department of Hematology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.

Exosomes, nanometer-sized membranous vesicles in body fluids, have emerged as promising non-invasive biomarkers for cancer diagnosis. However, the function of exosomes in diffuse large B-cell lymphoma (DLBCL) remains elusive. This study aimed to investigate the role of exosomal miR-107 in lymphomagenesis and explore its clinical significance. In this study, decreased exosomal miR-107, miR-375-3p, and upregulated exosomal miR-485-3p were detected in the plasma of DLBCL patients and showed potential diagnostic value. Downregulated miR-107 expression was associated with advanced Ann Arbor stage, high IPI score, LDH, and β-MG level in DLBCL patients. Overexpression of miR-107 by miR-107 Agomir significantly abrogated cell proliferation, induced apoptosis, and inhibited cell invasion , and repressed tumor growth . Moreover, the downregulation of miR-107 went in the opposite direction. The target genes of miR-107 were mainly enriched in the PI3K-Akt, Hippo, and AMPK signaling pathways. Notably, upregulated 14-3-3η (YWHAH) was suppressed by miR-107 in DLBCL, suggesting that miR-107 may restrain tumorigenesis by targeting 14-3-3η. In summary, this study unveils the function of miR-107 in lymphomagenesis, highlighting its potential as a diagnostic and prognostic indicator and as a new therapeutic target in the management of DLBCL.
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http://dx.doi.org/10.3389/fcell.2021.667800DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8111223PMC
April 2021

Developmental validation of the Microreader™ Y Prime Plus ID System: An advanced Y-STR 38-plex system for forensic applications.

Sci Justice 2021 May 30;61(3):260-270. Epub 2021 Jan 30.

Forensic Science Service of the Beijing Public Security Bureau, 100192 Beijing, China. Electronic address:

Y-STR is widely used in sexual assaults and familial searches of suspects. Here, we reported a novel 38-plex STR genotyping system designed for forensic applications. Microreader™ Y Prime Plus ID System (YPP) amplifies 38 loci in one reaction, including 29 loci from commonly used Yfiler® Plus PCR Amplification Kit & PowerPlex® Y23 System (DYS393, DYS570, DYS19, DYS392, DYS549, Y GATA H4, DYS460, DYS458, DYS481, DYS635, DYS448, DYS533, DYS449, DYS456, DYS389I, DYS390, DYS389Ⅱ, DYS438, DYS391, DYS439, DYS437, DYS385a/b, DYS643, DYS518, DYS576, DYF387S1a/b, and DYS627), 6 commonly used loci for the Y-STR database (DYS444, DYS447, DYS596, DYF404a/b, DYS527a/b, DYS557) and one Y-indel specific for the Chinese population. YPP is designed for different types of samples, such as blood card and swabs. In this work, YPP was validated following SWGDAM guidelines (2016) and guidelines from Ministry of Public Security of the People's Republic of China, including PCR-based, sensitivity, accuracy and precision, mixture, stability and inhibitor, and species specificity. The results indicate that the Microreader™ Y Prime Plus ID System is a powerful identification kit designed for forensic databases.
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http://dx.doi.org/10.1016/j.scijus.2021.01.003DOI Listing
May 2021

Dual-wavelength wide area illumination Raman difference spectroscopy for remote detection of chemicals.

Appl Opt 2021 Apr;60(12):3540-3548

Remote Raman instruments have become powerful analytical tools in some special environments. However, ambient daylight is the main limitation in the data acquisition process. To suppress daylight background interference and obtain a high signal-to-background ratio (SBR), we develop a dual-wavelength wide area illumination Raman difference spectroscopy (WAIRDS) system for daytime remote detection. In the WAIRDS system, a wide area illumination scheme and shifted-excitation Raman difference spectroscopy method are used to improve the reliability of collected Raman spectra. Measurements of polystyrene indicate that the WAIRDS system can be operated to obtain background-free Raman spectra under different levels of daylight background interference. The remote results show that the improvement in SBR is about three- to fivefold, and the system can work at distances of up to 9.2 m on a sunny afternoon. Moreover, to be close to the actual detection, the system is used for mineral and explosive raw material detection during daytime measurement. Measurements show that the WAIRDS system will be a useful tool for many remote applications in the future.
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http://dx.doi.org/10.1364/AO.422321DOI Listing
April 2021

Incidental Unruptured Intracranial Aneurysms Do Not Impact Outcome in Patients With Acute Cerebral Infarction.

Front Neurol 2021 26;12:613027. Epub 2021 Apr 26.

Department of Neurology, Affiliated Hospital of Yangzhou University, Yangzhou, China.

This study was to examine the patients with acute cerebral infarction (ACI) treated at a single center over 9 years and who underwent Unruptured intracranial aneurysm (UIA) screening by three-dimensional time-of-flight magnetic resonance angiography (3D-TOF-MRA), and to explore the factors associated with outcomes. The outcome was the modified Rankin scale (mRS) score at 90 days after stroke onset. The outcome was classified into a good outcome (mRS score of 0-2 points) and poor outcome (mRS score of 3-6 points). UIAs were found in 260 (6.5%) of 4,033 patients with ACI; 2,543 (63.1%) had a good outcome, and 1,490 (36.9%) had a poor outcome. There was no difference in outcomes between the two groups ( = 0.785). The multivariable analysis showed that age (OR = 1.009, 95%CI: 1.003-1.014, = 0.003), diabetes (OR = 1.179, 95%CI: 1.035-1.342, = 0.013), ischemic stroke history (OR = 1.451, 95%CI: 1.256-1.677, < 0.001), and baseline NIHSS score (OR = 1.034, 95%CI: 1.018-1.050, < 0.001) were independently associated with the 90-day outcomes in patients with ACI. The presence of incidental UIA was not associated with outcomes after ACI. Age, diabetes, ischemic stroke history, and baseline NIHSS score were independently associated with the early outcomes of patients with ACI.
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http://dx.doi.org/10.3389/fneur.2021.613027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107683PMC
April 2021

Significance of Serum Markers and Urinary Microalbumin in the Diagnosis of Early Renal Damage in Patients with Gout.

Clin Lab 2021 May;67(5)

Background: To explore the diagnostic value of changes in serum C-X-C Motif Chemokine Ligand 16 (CXCL16), cystatin C (CysC), cyclooxygenase-2 (COX-2), and urinary microalbumin (mALB) in patients with gout complicated by early renal damage.

Methods: A retrospective analysis of 47 patients with gout without complications and 48 patients with gout complicated by early renal damage was conducted in our hospital. A retrospective analysis was performed with 50 healthy people as controls. Serum IL-8, tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β), CXCL16, CysC, COX-2, and urine mALB levels were detected and analyzed, and the diagnostic efficacy of single factor and multifactor combined detection for early renal damage in patients with gout was analyzed and compared.

Results: Serum interleukin-8 (IL-8), TNF-α, IL-1β, CXCL16, CysC, COX-2, and urine mALB/Cr levels of patients with gout were significantly higher than those of healthy people (p < 0.01). Serum IL-8, TNF-α, IL-1β, CXCL16, CysC, COX-2 and urinary mALB/Cr levels in patients with gout complicated by early renal damage were significantly higher than those in patients with gout but without complications (p < 0.01). The sensitivity of CXCL16, CysC, COX-2, and mALB in diagnosing gout patients with early renal damage can reach 91.7%. CXCL16 and COX-2 were positively correlated with CysC and mALB/Cr, respectively, p < 0.01. The area under the ROC curve of CXCL16, CysC, COX-2, and mALB in the diagnosis of gout patients with early renal damage was 0.763, 0.832, 0.518, and 0.895, respectively.

Conclusions: CysC and mALB are sensitive indicators for the diagnosis of early renal damage. The combined diagnosis of CXCL16 and COX-2 can effectively improve the detection sensitivity of early renal damage in patients with gout.
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http://dx.doi.org/10.7754/Clin.Lab.2020.200722DOI Listing
May 2021