Publications by authors named "Yi Wang"

7,102 Publications

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Volumetric changes in specific neurofunctional subfields of the hippocampus in major depressive disorder.

Neuroreport 2022 Jun 11;33(9):380-385. Epub 2022 May 11.

Shanghai TCM-Integrated Hospital, Shanghai University of Traditional Chinese Medicine.

Objective: There is evidence that hippocampal volume is abnormal in patients with major depressive disorder (MDD), but there have been no studies on volumetric changes in different subfields based on functional topography. This was investigated in the present study by comparing hippocampal neurofunctional subfield volumes between MDD patients and healthy control (HC) subjects.

Methods: Patients with MDD (n = 44) and HCs (n = 27) recruited at Shanghai Traditional Chinese Medicine Integrated Hospital underwent a T1-weighted anatomical MRI scan in the sagittal orientation, and the data were used to calculate hippocampal subfield volumes. Logistic regression was used to evaluate the association between the volumes and risk of MDD. A nomogram for predicting MDD risk based on volume changes in different subfields was developed, and its predictive power was evaluated by calculating the concordance (C)-index.

Results: Compared with HCs, MDD patients showed reduced volume in hippocampal neurofunctional subfields, specifically in left (L)1, right (R)1, and R2 (related to emotion) and L2, L3, and R4 (related to cognition and perception). The logistic regression analysis revealed that the risk of MDD was 4.59-, 5.8-, 8.33-, and 6.92-fold higher with atrophies of L1, L2, L3, and R4, respectively. A nomogram for predicting MDD risk was developed based on age; sex; and hippocampal L1, L2, L3, and R4 subfield volumes and showed good accuracy, with a C-index of 0.784.

Conclusion: Volumetric changes in the neurofunctional subfield of the hippocampus are potential imaging markers that can predict the occurrence of MDD.
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http://dx.doi.org/10.1097/WNR.0000000000001794DOI Listing
June 2022

Exploration of Stock Portfolio Investment Construction Using Deep Learning Neural Network.

Authors:
Zizheng Xie Yi Wang

Comput Intell Neurosci 2022 10;2022:7957097. Epub 2022 May 10.

Economics and Management School, Wuhan University, Wuhan 430072, China.

To study the intelligent and efficient stock portfolio in China's financial market, based on the relevant theories such as deep learning (DL) neural network (NN) and stock portfolio, this study selects 111 stable stocks from the constituent stocks of the China Security Index (CSI) 300 from January 1, 2018, to December 31, 2021, as the research samples. Then, it analyzes these research samples and imports the relevant data of 111 stocks into the DL NN model. The corresponding prediction results of stock prices are obtained. Finally, the stock portfolio model based on DL NN is compared with the data results of the Shanghai Stock Exchange (SSE) 50 Index and CSI 500 Index. The results show that the closing prices of the selected 111 stocks are relatively stable and fluctuate up and down around the horizontal axis, and the positive and negative returns are relatively balanced, roughly between -5% and 5%. There is a phenomenon of fluctuation aggregation to a certain extent. Comparing the prediction results of different models reveals that the prediction results of model are closest to the actual stock price trend. Comparing the relevant returns of the proposed stock portfolio with other stocks uncovers that the annualized return of the stock portfolio based on the DL NN model is 47.44%. The sharp ratio is 1.52, the maximum pullback is 18.15%, the monthly excess return is 3.11%, and the information ratio is 0.82. Compared with other indexes, the proposed stock portfolio shows the best results. Therefore, the proposal of the stock portfolio based on DL NN provides a theoretical basis for the development of the financial field in the future.
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http://dx.doi.org/10.1155/2022/7957097DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113895PMC
May 2022

Exercise Modifies the Transcriptional Regulatory Features of Monocytes in Alzheimer's Patients: A Multi-Omics Integration Analysis Based on Single Cell Technology.

Front Aging Neurosci 2022 3;14:881488. Epub 2022 May 3.

Huashan Hospital, Fudan University, Shanghai, China.

Monocytes have been reported to be important mediators of the protective effect of exercise against the development of Alzheimer's disease (AD). This study aims explored the mechanism by which monocytes achieve this. Using single cell transcriptome analysis, results showed that CD14 + and CD16 + monocytes interacted with other cells in the circulating blood. , , , and , the key ligand-receptor-related genes, were found to be differentially expressed between exercise-treated and AD patients. The SCENIC analysis was performed to identify individual clusters of the key transcription factors (TFs). Nine clusters (M1-M9) were obtained from the co-expression network. Among the identified TFs, , , and were found to be differentially expressed in AD. Moreover, the M4 cluster to which , , and belonged was defined as the signature cluster for AD phenotype. Differential analysis by bulkRNA-seq revealed that the expression of , , and were all upregulated after exercise ( < 0.05). And , , , and that were identified in M4 clusters may be the TFs that regulate , , and in exercise prescription. After that, , , , , , , and formed a regulatory network in the ERADMT gene set, and all of them were mechanistically linked. The ERADMT gene set has been found to be a potential risk marker for the development of AD and can be used as an indicator of compliance to exercise therapy in AD patients. Using single-cell integration analysis, a network of exercise-regulating TFs in monocytes was constructed for AD disease. The constructed network reveals the mechanism by which exercise regulated monocytes to confer therapeutic benefits against AD and its complications. However, this study, as a bioinformatic research, requires further experimental validation.
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http://dx.doi.org/10.3389/fnagi.2022.881488DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9110789PMC
May 2022

Effect of the Porcine STC-1 Gene on Autophagy and Mitochondrial Function as Induced by Serum Starvation.

Biochem Genet 2022 May 19. Epub 2022 May 19.

College of Veterinary Medicine, Yangzhou University/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Yangzhou, 225009, Jiangsu, People's Republic of China.

Stanniocalcin-1 (STC-1) is a glycoprotein hormone involved in calcium/phosphorus metabolism and direct inhibition of bone and muscle growth. The aim of this study was to investigate the STC-1 gene with respect to the regulatory mechanisms of porcine growth metabolic pathways involving autophagy. Western blotting was used to detect the expression of autophagy and mitochondrial function-related proteins, and flow cytometry was used to detect mitochondrial function-related. Changes in the autophagosome and mitochondrial were observed by electron microscopy. The expression of the autophagy-related proteins was detected by confocal microscopy. The results showed that Pink1, Parkin and LC3B expression was increased; SQSTM1/P62 expression was reduced. Electron microscopy revealed that the cells in the serum starvation group all produced autophagosomes. The fluorescence intensity of GFP-LC3B and GFP-Parkin increased. The Bax/Bcl-2 ratio, Pink1 and Parkin protein levels were profoundly reduced in the STC-KO. In addition, the increase in Mfn2, OPA1, DRP1 and LC3B proteins was attenuated; the increase in the apoptosis rate and amount of active oxygen was attenuated; the decrease in membrane potential; the decrease in ATP was reversed; the fluorescence intensity of GFP-LC3B and GFP-Parkin was increased. These results indicate that autophagy can be caused by serum starvation. Knocking out the porcine STC-1 gene had an obvious antiapoptotic effect on cells, the inhibition of serum starvation-induced autophagy. This is the first study to show that the porcine STC-1 gene confers self-protection in the absence of nutrients. To provide a theoretical basis for studying the effect of STC-1 on pig growth and development.
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http://dx.doi.org/10.1007/s10528-022-10233-4DOI Listing
May 2022

Activation of waste paper: Influence of varied chemical agents on product properties.

Waste Manag 2022 May 16;146:94-105. Epub 2022 May 16.

School of Material Science and Engineering, University of Jinan, Jinan 250022, PR China. Electronic address:

Waste paper (WP) is rich in cellulose, which can be activated to produce porous carbon, bio-oil, and combustible gases. During chemical activation of WP, the use of varied chemical agents not only generates activated carbon of distinct pore structure but also bio-oil/gases of different property. In this study, the activation of WP with varied chemical agents was conducted. The distinct characteristics of activated carbon and also bio-oil/gases were correlated with the different nature of the used chemical agents. The results indicated that HPO and ZnCl catalyzed polymerization reactions for producing more bio-oil while less gases owing to their Brønsted and Lewis acidic sites. KCO showed high activity for cracking/gasification reactions, forming bio-oil with higher abundance of organics with smaller π-conjugated structures. In addition, ZnCl could create a very coarse porous structure with abundant macropores via destroying fiber structure in WP and promoting the growth of graphitic crystals. In comparison, KCO hindered the aromatization and facilitated the formation of amorphous activated carbon. KCO and ZnCl were much more effective than HPO for creating micropores and mesopores from WP, the derived activated carbon showed superior performances as the electrode of supercapacitor and adsorbent for adsorption of oxytetracycline from aqueous solution. In addition, KCO as activating agent showed lower environmental impact than ZnCl in terms of energy consumption, environmental pollution and the greenhouse effect.
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http://dx.doi.org/10.1016/j.wasman.2022.05.003DOI Listing
May 2022

Soil and foliar exposure of soybean (Glycine max) to Cu: Nanoparticle coating-dependent plant responses.

NanoImpact 2022 May 11;26:100406. Epub 2022 May 11.

Environmental Science and Engineering Ph.D. Program, The University of Texas at El Paso, 500 West University Ave., El Paso, TX 79968, USA; Department of Chemistry and Biochemistry, The University of Texas at El Paso, 500 West University Ave., El Paso, TX 79968, USA. Electronic address:

In this study, we investigated the effects of citric acid (CA) coated copper oxide nanoparticles (CuO NPs) and their application method (foliar or soil exposure) on the growth and physiology of soybean (Glycine max). After nanomaterials exposure via foliar or soil application, Cu concentration was elevated in the roots, leaves, stem, pod, and seeds; distribution varied by plant organ and surface coating. Foliar application of CuO NPs at 300 mg/L and CuO-CA NPs at 75 mg/L increased soybean yield by 169.5% and 170.1%, respectively. In contrast, foliar and soil exposure to ionic Cu with all treatments (75 and 300 mg/L) had no impact on yield. Additionally, CuO-CA NPs at 300 mg/L significantly decreased Cu concentration in seeds by 46.7%, compared to control, and by 44.7%, compared to equivalent concentration of CuO NPs. Based on the total Cu concentration, CuO NPs appeared to be more accessible for plant uptake, compared to CuO-CA NPs, inducing a decrease in protein content by 56.3% and inhibiting plant height by 27.9% at 300 mg/kg under soil exposure. The translocation of Cu from leaf to root and from the root to leaf through the xylem was imaged by two-photon microscopy. The findings indicate that citric acid coating reduced CuO NPs toxicity in soybean, demonstrating that surface modification may change the toxic properties of NPs. This research provides direct evidence for the positive effects of CuO-CA NPs on soybean, including accumulation and in planta transfer of the particles, and provides important information when assessing the risk and the benefits of NP use in food safety and security.
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http://dx.doi.org/10.1016/j.impact.2022.100406DOI Listing
May 2022

Protective effects of lavender oil on sepsis-induced acute lung injury via regulation of the NF-κB pathway.

Pharm Biol 2022 Dec;60(1):968-978

No.1 Cardiovascular Disease Department, Heilongjiang Chinese Medical University No.1 Affiliated Hospital, Harbin, Heilongjiang, China.

Context: Lavender oil (Lav) from L. (Lamiacease) exhibits antioxidative and anti-inflammatory properties against various diseases.

Objective: The study explores the effect of Lav pre-treatment on sepsis-induced acute lung injury (ALI).

Materials And Methods: Sprague-Dawley rats were assigned into Sham, caecal ligation and puncture (CLP), CLP + Lav (200, 400, and 800 mg/kg) groups. Lav was administered by gavage, once a day, for 7 days. Histological analysis was performed using haematoxylin and eosin staining. Cytokine and nitrite levels were detected by enzyme-linked immunosorbent assay kits and Griess reagent. Gene and protein expression were tested by quantitative real-time polymerase chain reaction and western blot.

Results: The levels of tumour necrosis factor-α (BALF: 64%, serum: 59%), interleukin (IL)-1β (BALF: 63%, serum: 66%) and IL-6 (BALF: 54%, serum: 59%), and nitrite (40%) and inducible nitric oxide synthase (51%), and the level of myeloperoxidase (66%) and malondialdehyde (59%), and cleaved-caspase 3 (84%) and Bax expression (74%) induced by CLP were decreased when given Lav. Additionally, the level of superoxide dismutase (211%) and glutathione (139%), and the expression of Bcl-2 (980%) induced by CLP were increased when given Lav. The increased p-nuclear factor (NF)-κB/NF-κB (72%) and p-inhibitor of κBα (IκBα)/IκBα (77%) induced by CLP could be reversed by Lav.

Discussion And Conclusions: Lav pre-treatment might protect rats from sepsis-induced ALI via deactivation of the NF-κB pathway. Our research demonstrated the regulatory mechanisms of Lav in sepsis-induced ALI and can provide a theoretical basis for the use of Lav in the treatment of sepsis-induced ALI.
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http://dx.doi.org/10.1080/13880209.2022.2067570DOI Listing
December 2022

 (Primulaceae), a new species from Yunnan, China.

PhytoKeys 2022 11;194:15-22. Epub 2022 Apr 11.

Forestry and Grassland Bureau, Lincang 677000, Yunnan, China Forestry and Grassland Bureau Lincang China.

from SW Yunnan, China, is described as a species new to science and illustrated. The systematic placement of this new species is also discussed based on an nrITS molecular tree. It is morphologically most similar to , but differs from the latter in its racemose inflorescence, green calyx tube, pink to pink rose corolla, stamens at 1/3 length above the base of the corolla tube and applanate globose capsule.
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http://dx.doi.org/10.3897/phytokeys.194.81335DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9016029PMC
April 2022

Functionalized Macrophage Exosomes with Panobinostat and PPM1D-siRNA for Diffuse Intrinsic Pontine Gliomas Therapy.

Adv Sci (Weinh) 2022 May 18:e2200353. Epub 2022 May 18.

CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China, Beijing, 100190, P. R. China.

Diffuse intrinsic pontine glioma (DIPG) is a rare and fatal pediatric brain tumor. Mutation of p53-induced protein phosphatase 1 (PPM1D) in DIPG cells promotes tumor cell proliferation, and inhibition of PPM1D expression in DIPG cells with PPM1D mutation effectively reduces the proliferation activity of tumor cells. Panobinostat effectively kills DIPG tumor cells, but its systemic toxicity and low blood-brain barrier (BBB) permeability limits its application. In this paper, a nano drug delivery system based on functionalized macrophage exosomes with panobinostat and PPM1D-siRNA for targeted therapy of DIPG with PPM1D mutation is prepared. The nano drug delivery system has higher drug delivery efficiency and better therapeutic effect than free drugs. In vivo and in vitro experimental results show that the nano drug delivery system can deliver panobinostat and siRNA across the BBB and achieve a targeted killing effect of DIPG tumor cells, resulting in the prolonged survival of orthotopic DIPG mice. This study provides new ideas for the delivery of small molecule drugs and gene drugs for DIPG therapy.
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http://dx.doi.org/10.1002/advs.202200353DOI Listing
May 2022

Highly Efficient Genome Editing in Clostridium difficile Using the CRISPR-Cpf1 System.

Methods Mol Biol 2022 ;2479:175-187

Department of Biosystems Engineering, Auburn University, Auburn, AL, USA.

Clostridium difficile is often the primary cause of nosocomial diarrhea, leading to thousands of deaths annually worldwide. The availability of an efficient genome editing tool for C. difficile is essential to understanding its pathogenic mechanism and physiological behavior. Here, we describe a streamlined CRISPR-Cpf1-based protocol to achieve precise genome editing in C. difficile with high efficiencies. Our work highlighted the first application of CRISPR-Cpf1 for genome editing in C. difficile, which are both crucial for understanding pathogenic mechanism of C. difficile and developing strategies to fight against C. difficile infection (CDI). In addition, for the DNA cloning, we developed a one-step-assembly protocol along with a Python-based algorithm for automatic primer design, shortening the time for plasmid construction to half that of conventional procedures. Approaches we developed herein are easily and broadly applicable to other microorganisms. Our results provide valuable guidance for establishing CRISPR-Cpf1 as a versatile genome engineering tool in prokaryotic cells.
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http://dx.doi.org/10.1007/978-1-0716-2233-9_12DOI Listing
May 2022

Biodegradable Microplastics: A Review on the Interaction with Pollutants and Influence to Organisms.

Bull Environ Contam Toxicol 2022 May 18. Epub 2022 May 18.

Nanjing Kaver Scientific Instruments, Co., Ltd, Nanjing, 210042, Jiangsu, China.

Biodegradable plastics attract public attention as promising substitutes for traditional nondegradable plastics which have caused the serious white pollution problem due to their persistence. However, even for biodegradable plastics, natual conditions for the rapid and complete degradation are rare. Even more serious is that biodegradable plastics might be disintegrated into microplastics more rapidly than tranditional plastics, emerging as another threat to the environment. Similar to traditional microplastics, biodegradable microplastics could adsorb many pollutants by various physicochemical effects and release additives. Biodegradable microplastics have been confirmed to be toxic to the organisms as particle matter and the vector as pollutants. Under some conditions, biodegradable microplastics may pose more severe negative impacts on the organisms. With the fierely increasing trend to replace the nondegradable plastic commodities with biodegradable ones, it is necessary to evaluate whether biodegradable plastics and the generated microplastics would alleviate plastic pollution or induce greater ecological impacts.
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http://dx.doi.org/10.1007/s00128-022-03486-7DOI Listing
May 2022

ROS-Targeted Depression Therapy via BSA-Incubated Ceria Nanoclusters.

Nano Lett 2022 May 18. Epub 2022 May 18.

Center for Translational Neurodegeneration and Regenerative Therapy, Tongji Hospital affiliated to Tongji University School of Medicine, Shanghai 200065, China.

Depression is one of the most fatal mental diseases, and there is currently a lack of efficient drugs for the treatment of depression. Emerging evidence has indicated oxidative stress as a key pathological feature of depression. We targeted reactive oxygen species (ROS) and synthesized [email protected] nanoclusters as a novel antidepression nanodrug a convenient, green, and highly effective bovine serum albumin (BSA) incubation strategy. [email protected] has ultrasmall size (2 nm) with outstanding ROS scavenging and blood-brain barrier crossing capacity, rapid metabolism, and negligible adverse effects and . [email protected] administration alleviates depressive behaviors and depression-related pathological changes of the chronic restraint stress-induced depressive model, suggesting promising therapeutic effects of [email protected] for the treatment of depression. Our study proved the validity by directly using nanodrugs as antidepression drugs instead of using them as a nanocarrier, which greatly expands the application of nanomaterials in depression treatment.
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http://dx.doi.org/10.1021/acs.nanolett.2c01334DOI Listing
May 2022

Neighborhood Environment and Depressive Symptoms among Chinese Older Immigrants in the U.S.: The Mediation Effects of Coping Resources.

Gerontologist 2022 May 18. Epub 2022 May 18.

Institute for Health, Health Care Policy and Aging Research, Rutgers, The State University of New Jersey, New Brunswick, New Jersey, USA.

Background And Objectives: Studies have shown that neighborhood environment shapes older Americans' aging experience and health. However, it remains largely unknown whether and how neighborhood environment influences the wellbeing of older Asian Immigrants. Guided by the neighborhood stress process model, this study aims to investigate 1) the associations between neighborhood environmental stressors and depression among Chinese older immigrants, and 2) the potential mediation effects of intrapersonal (sense of mastery, sense of hopefulness) and interpersonal coping (social engagement) resources in such associations.

Research Design And Methods: This study analyzed data collected from 2,801 Chinese older immigrants in the greater Chicago area. Structural equation modeling (SEM) with bootstrap resampling was used to fit path models on neighborhood environmental stressor, intra- and interpersonal coping resources, and depression.

Results: Findings showed that neighborhood social disintegration and physical disorder were associated with more depressive symptoms directly and indirectly via lower intra- and inter-personal coping resources. Specifically, older immigrants living in neighborhoods with greater social disintegration reported lower sense of mastery and social engagement, which in turn were associated with more depressive symptoms (partial mediation). Older immigrants living in neighborhoods with greater physical disorder reported lower sense of hopefulness and mastery, which subsequently were associated with more depressive symptoms (full mediation).

Discussion And Implications: The findings showed that neighborhood environmental stressors are risk factors for mental health of older Chinese immigrants and coping resources may serve as pathways of the associations. The implications for future research and practice were discussed.
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http://dx.doi.org/10.1093/geront/gnac065DOI Listing
May 2022

Reliability and validity of a Chinese Version of the Lysholm Score and Tegner Activity Scale for Knee Arthroplasty.

J Rehabil Med 2022 May 18. Epub 2022 May 18.

Department of Sports Medicine, Peking University Third Hospital, Institute of Sports Medicine of Peking University, Beijing Key Laboratory of Sports Injuries, Beijing, China.

Objective: To verify the reliability and validity of Chinese versions of the Lysholm score and the Tegner activity scale for knee arthroplasty.

Methods: Sixty-four patients undergoing total knee arthroplasty and 28 healthy volunteers were included in this study. Participants were divided into 4 groups: a pre-operation group; a 3 months post-operation group; a 1-year post-operation group; and a control group of healthy volunteers. Participants completed the Lysholm score and Tegner activity scale twice over a period of 3-7 days.

Results: The intraclass correlation coefficients of the Lysholm score and Tegner scale were both relatively high, at 0.99 and 0.97, respectively. Moreover, the Cronbach's alpha of the Lysholm score was 0.71. The items "locking" and "instability" differed slightly between groups (Kruskal-Wallis: for locking, χ2 (p) = 13.48, p = 0.0037; for instability, χ2 (p) = 4.32, p = 0.2292).

Conclusion: The simplified-Chinese versions of the Lysholm score and the Tegner scale are applicable for use with patients undergoing total knee arthroplasty, and have relatively high validity and reliability. The items "locking" and "instability" should be combined with clinical data to make the Lysholm score more suitable for assessment of total knee arthroplasty.
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http://dx.doi.org/10.2340/jrm.v54.2304DOI Listing
May 2022

Pulmonary CT scan of white rabbits using the selective photon shield technique of the third-generation force CT.

J Radiol Prot 2022 May 17. Epub 2022 May 17.

Hunan Provincial People's Hospital, Changsha, 410005, CHINA.

Objective: This study aims to optimize the protocol for low-dose pulmonary computed tomography (CT) scanning of infants by studying the effects of the selective photon shield (SPS) technique of the third-generation dual-source CT (DSCT) on the image quality and radiation dose of chest CT in white rabbits under different tube currents.

Method: Twelve white rabbits of similar weight to infants were selected and randomly divided into Experimental group and Control group. The Experimental groups (A1-A5) were scanned at low dose by the third-generation DSCT using SPS under different tube current*time (60, 50, 40, 30, and 20 mAs). The Control group (B) was scanned under a conventional tube voltage (100 kV) and current*time (20 mAs). Advanced model iterative reconstruction at strength 3 was used for objective and subjective evaluation of the image quality and radiation dose of the lung and mediastinal windows. With the standard deviation of the air in the trachea as image noise, the signal-to-noise ratio (SNR), contrast-to-noise ratio, and CT values of each site were evaluated. Radiation doses were compared using volume CT dose index, dose length product, and effective dose.

Result: The differences in subjective image quality between group A2 and B were not statistically significant (P = 0.34). The differences in SNR of lung and mediastinal windows between group A2 and B were not statistically significant (P > 0.05). The radiation dose of group A2 was 83.2% lower than that of group B.

Conclusion: SPS of the third-generation DSCT under 50 mAs might be applied in pulmonary CT examination of infants.
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http://dx.doi.org/10.1088/1361-6498/ac7089DOI Listing
May 2022

Role of Neighborhood Physical Disorder and Social Cohesion on Racial and Ethnic Disparities in Dementia Risk.

Authors:
Roger Wong Yi Wang

J Aging Health 2022 May 17:8982643221101352. Epub 2022 May 17.

School of Social Work, University of Iowa, Iowa City, IA, USA.

To analyze how neighborhood physical disorder and social cohesion are associated with racial and ethnic disparities in dementia risk. Nine years of data (2011-2019) were retrieved from the National Health and Aging Trends Study, a nationally representative U.S. older adult (age 65+) sample. Cox regression analyzed time to dementia diagnosis using composite scores for neighborhood physical disorder and social cohesion. Higher baseline neighborhood physical disorder (Adjusted Hazard Ratio [aHR]=1.11, 95% Confidence Interval [CI]=1.01-1.23) and increased disorder at follow-up (aHR=1.10, 95% CI=1.01-1.19) significantly increased dementia risk. Hispanic older adults with higher physical disorder at baseline (aHR=0.62, 95% CI=0.49-0.79) and follow-up (aHR=0.81, 95% CI=0.67-0.98) had a significantly decreased dementia risk. There were no significant associations for social cohesion. Physical but not social neighborhood characteristics are associated with dementia risk. Future research is needed to understand protective mechanisms for dementia among Hispanic older adults in neighborhoods with high physical disorder.
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http://dx.doi.org/10.1177/08982643221101352DOI Listing
May 2022

Electrochemical-Promoted Nickel-Catalyzed Reductive Allylation of Aryl Halides.

Org Lett 2022 May 17. Epub 2022 May 17.

State Key Laboratory of Coordination Chemistry, Jiangsu Key Laboratory of Advanced Organic Materials, Collaborative Innovation Center of Advanced Microstructures, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.

Compared with conventional reductive coupling, reductive coupling under electrochemical conditions without external reductants is greener, milder, and more efficient and is of increasing interest to organic chemists. In this work, we report the sacrificial anode, nickel-catalyzed electrochemical allylation reaction of aryl and alkyl halides. The reaction can be applied to a range of allylation reagents such as trifluoroalkenes, oxalates, and acetates.
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http://dx.doi.org/10.1021/acs.orglett.2c01247DOI Listing
May 2022

Network Pharmacology Deciphers the Action of Bioactive Polypeptide in Attenuating Inflammatory Osteolysis via the Suppression of Oxidative Stress and Restoration of Bone Remodeling Balance.

Oxid Med Cell Longev 2022 14;2022:4913534. Epub 2022 Apr 14.

Department of Joint Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, China.

Oxidative stress involves enormously in the development of chronic inflammatory bone disease, wherein the overproduction of reactive oxygen species (ROS) negatively impacts the bone remodeling via promoting osteoclastogenesis and inhibiting osteogenesis. Lacking effective therapies highlights the importance of finding novel treatments. Our previous study screened a novel bioactive peptide D7 and demonstrated it could enhance the cell behaviors and protect bone marrow mesenchymal stem cells (BMSCs). Since BMSCs are progenitor cells of osteoblast (OB), we therefore ask whether D7 could also protect against the progress of inflammatory osteolysis. To validate our hypothesis and elucidate the underlying mechanisms, we first performed network pharmacology-based analysis according to the molecule structure of D7, and then followed by pharmacological evaluation on D7 by in vitro lipopolysaccharide(LPS)-induced models. The result from network pharmacology identified 20 candidate targets of D7 for inflammatory osteolysis intervention. The further analysis of Gene Ontology (GO)/KEGG pathway enrichment suggested the therapeutic effect of D7 may primarily affect osteoclast (OC) differentiation and function during the inflammatory osteolysis. Through validating the real effects of D7 on OC and OB as postulated, results demonstrated suppressive effects of D7 on LPS-stimulated OC differentiation and resorption, via the inhibition on OC marker genes. Contrarily, by improving the expression of OB marker genes, D7 displayed promotive effects on OB differentiation and alleviated LPS-induced osteogenic damage. Further mechanism study revealed that D7 could reduce LPS-induced ROS formation and strengthen antioxidants expressions in both OC and OB precursors, ameliorating LPS-triggered redox imbalance in bone remodeling. Taken together, our findings unveiled therapeutic effects of D7 against LPS-induced inflammatory osteolysis through the suppression of oxidative stress and the restoration of the bone remodeling process, providing a new therapeutic candidate for chronic inflammatory bone diseases.
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http://dx.doi.org/10.1155/2022/4913534DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9107052PMC
May 2022

Discovering the direct evidence of photocatalytic sterilization mechanism on bimetallic sulfides heterostructures.

J Colloid Interface Sci 2022 May 7;623:182-195. Epub 2022 May 7.

School of Petrochemical Engineering, Liaoning Shihua University, Fushun 113001, China.

Despite the development of sterilization, the study of reactive oxygen species (ROS) based disinfection mechanism is still under restrictions due to the lack of direct evidence. In this work, DiI is used to explore the sterilization mechanism in-depth. The mechanism is directly proven to be that the generated ROS would seriously damage the lipid of the cell membrane. It provides direct evidence for the destruction of cell membrane for the first time. These ROS can cause the death of more than 99% of both Gram-negative and Gram-positive bacteria within 120 min under visible light. What's more, MoS/MS (MS = MnS, CuS, and CeS) are successfully synthesized by employing Mo/M-MOF (M = Mn, Cu, and Ce) as precursors. Since bigger Fermi lever difference and smaller work function bring better photocatalytic activity, MoS/MnS composites display superb photocatalytic activity than others. Therefore, the as-prepared materials can be widely used in environmental protection, biotechnology, and clinical therapy.
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http://dx.doi.org/10.1016/j.jcis.2022.05.019DOI Listing
May 2022

High-Entropy Perovskite as a High-Performing Chromium-Tolerant Cathode for Solid Oxide Fuel Cells.

ACS Appl Mater Interfaces 2022 May 16. Epub 2022 May 16.

Mechanical & Aerospace Engineering Department, West Virginia University, Morgantown, West Virginia 26506, United States.

To achieve chromium tolerance and high performance, a new series of high-entropy perovskites (HEPs) are investigated as cathode materials for solid oxide fuel cells (SOFCs). Multiple rare-earth, alkaline-earth, and high-order transition metal elements are used for the A-site of this ABO structure. A pure phase is achieved through the designed combination of different elements in seven out of eight candidates. Due to the retaining of alkaline-earth elements Sr and/or Ba, the electrical conductivities of these HEPs are in the order of 100 S/cm at 550-700 °C, a value that can practically eliminate the electronic resistance of the porous cathode. Three out of eight candidates show similar or better performance than the (LaSr)(CoFe)O (LSCF) benchmark. It is found that A-site elements can cast a substantial influence on the overall performance even with a change as small as 10% of the total cations. It seems that each element has its individual "phenomenal activity" that can be transferred from one candidate to the other in the general setting of the perovskite structure, leading to the best candidate by using the three most active elements simultaneously at the A-site. Excellent Cr tolerance has been observed on the (LaSrPrYBa)CoFeO sample, showing degradation of only 0.25%/kh during a 41 day operation in the presence of Cr, while LSCF increases by 100% within the first day in the same condition. X-ray photoelectron spectroscopy discovers no Sr segregation as LSCF is found in this HEP; rather, the active element Y takes more A-sites on the outermost layer after long-term operation.
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http://dx.doi.org/10.1021/acsami.2c03657DOI Listing
May 2022

Murphy Scale: A locational equivalent intensity scale for hazard events.

Risk Anal 2022 May 16. Epub 2022 May 16.

Department of Earth, Marine and Environmental Sciences, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.

Empirical cross-hazard analysis and prediction of disaster vulnerability, resilience, and risk requires a common metric of hazard strengths across hazard types. In this paper, the authors propose an equivalent intensity scale for cross-hazard evaluation of hazard strengths of events for entire durations at locations. The proposed scale is called the Murphy Scale, after Professor Colleen Murphy. A systematic review and typology of hazard strength metrics is presented to facilitate the delineation of the defining dimensions of the proposed scale. An empirical methodology is introduced to derive equivalent intensities of hazard events on a Murphy Scale. Using historical data on impacts and hazard strength indicators of events from 2013 to 2017, the authors demonstrate the utility of the proposed methodology for computing the equivalent intensities for earthquakes and tropical cyclones. As part of a new area of research called hazard equivalency, the proposed Murphy Scale paves the way toward creating multi-hazard hazard maps. The proposed scale can also be leveraged to facilitate hazard communication regarding past and future local experiences of hazard events for enhancing multi-hazard preparedness, mitigation, and emergency response.
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http://dx.doi.org/10.1111/risa.13933DOI Listing
May 2022

Reversible regulation of enzyme-like activity of molybdenum disulfide quantum dots for colorimetric pharmaceutical analysis.

J Pharm Anal 2022 Feb 31;12(1):113-121. Epub 2021 Mar 31.

Chongqing Research Center for Pharmaceutical Engineering, College of Pharmacy, Chongqing Medical University, Chongqing, 400016, China.

Regulating the catalytic activity of nanozymes is significant for their applications in various fields. Here, we demonstrate a new strategy to achieve reversible regulation of the nanozyme's activity for sensing purpose. This strategy involves the use of zero-dimensional MoS quantum dots (MQDs) as the building blocks of nanozymes which display very weak peroxidase (POD)-like activity. Interestingly, such POD-like activity of the MQDs largely enhances in the presence of Fe while diminishes with the addition of captopril thereafter. Further investigations identify the mechanism of Fe-mediated aggregation-induced enhancement of the POD-like activity and the inhibitory effect of captopril on the enhancement, which is highly dependent on their concentrations. Based on this finding, a colorimetric method for the detection of captopril is developed. This sensing approach exhibits the merits of simplicity, rapidness, reliability, and low cost, which has been successfully applied in quality control of captopril in pharmaceutical products. Moreover, the present sensing platform allows smartphone read-out, which has promising applications in point-of-care testing devices for clinical diagnosis and drug analysis.
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http://dx.doi.org/10.1016/j.jpha.2021.03.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9073247PMC
February 2022

Deciphering bioactive compounds of complex natural products by tandem mass spectral molecular networking combined with an aggregation-induced emission based probe.

J Pharm Anal 2022 Feb 28;12(1):129-135. Epub 2020 Nov 28.

Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, 310058, China.

Natural products are great treasure troves for the discovery of bioactive components. Current bioassay guided fractionation for identification of bioactive components is time- and workload-consuming. In this study, we proposed a robust and convenient strategy for deciphering the bioactive profile of natural products by mass spectral molecular networking combined with rapid bioassay. As a proof-of-concept, the strategy was applied to identify angiotensin converting enzyme (ACE) inhibitors of Fangjihuangqi decoction (FJHQD), a traditional medicine clinically used for the treatment of heart failure. The chemical profile of FJHQD was comprehensively revealed with the assistance of tandem mass spectral molecular networking, and a total of 165 compounds were identified. With characterized constituents, potential clinical applications of FJHQD were predicted by Bioinformatics Analysis Tool for Molecular mechANism of Traditional Chinese Medicine, and a range of cardiovascular related diseases were significantly enriched. ACE inhibitory activities of FJHQD and its constituents were then investigated with an aggregation-induced emission based fluorescent probe. FJHQD exhibited excellent ACE inhibitory effects, and a bioactive molecular network was established to elucidate the ACE inhibitory profile of constituents in FJHQD. This bioactive molecular network provided a panoramic view of FJHQD's ACE inhibitory activities, which demonstrated that flavones from Astragali Radix and Glycyrrhizae Radix et Rhizoma, saponins from Astragali Radix, and sesquiterpenoids from Atractylodis Macrocephalae Rhizoma were principal components responsible for this effect of FJHQD. Among them, four novel ACE inhibitors were the first to be reported. Our study indicated that the proposed strategy offers a useful approach to uncover the bioactive profile of traditional medicines and provides a pragmatic workflow for exploring bioactive components.
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http://dx.doi.org/10.1016/j.jpha.2020.11.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9073139PMC
February 2022

Traditional Chinese Medicine Tanreqing Targets Both Cell Division and Virulence in .

Front Cell Infect Microbiol 2022 27;12:884045. Epub 2022 Apr 27.

Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing, China.

has been recognized as an important human pathogen and poses a serious health threat worldwide. With the advent of antibiotic resistance, such as the increased number of methicillin-resistant (MRSA), there is an urgent need to develop new therapeutical agents. In this study, Chinese traditional medicine Tanreqing (TRQ) has been used as an alternative treating agent against MRSA and we aim to unravel the mode of action of TRQ underlying MRSA inhibition. TRQ treatment affected numerous gene expression as revealed by RNA-seq analysis. Meanwhile, TRQ targeted cell division to inhibit cell growth as shown by illumination microscopy. Besides, we confirmed that TRQ downregulates the expression of virulence factors such as hemolysin and autolysin. Finally, we used a murine model to demonstrate that TRQ efficiently reduces bacterial virulence. Altogether, we have proved TRQ formula to be an effective agent against infections.
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http://dx.doi.org/10.3389/fcimb.2022.884045DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9093593PMC
May 2022

Epilepsy and Molecular Phenotype Affect the Neurodevelopment of Pediatric Angelman Syndrome Patients in China.

Front Psychiatry 2022 28;13:886028. Epub 2022 Apr 28.

Department of Neurology, Children's Hospital of Fudan University, Shanghai, China.

Objective: This study investigated the mental development of children with Angelman syndrome (AS) in China and evaluated the relationship between neurodevelopment and molecular subtype, age, epilepsy, and sex using the Chinese version of the Griffith Mental Development Scale (GMDS-C) to provide detailed baseline data regarding neurodevelopment with AS in China.

Methods: Participants were recruited from the AS Natural History Study. The GMDS-C was used to evaluate all participants' mental age and developmental quotients. The general quotient (GQ) and quotients of five subscales (sports, personal-social, auditory language, eye-hand coordination, and comprehensive performance) were calculated.

Results: A total of 119 children (average age: 42.12 months; range, 7.5-95.5 months) with a genetic diagnosis of AS were enrolled. The median GQ score of the GMDS was 29.6 points (95% confidence interval, 28.6-33.25). The children had relatively good locomotor and personal-social skills but poor language skills. Overall, 89% (106/119) had mental ages younger than 24 months for all five subscales. The non-deletion group (i.e., without deletion in chromosome 15q11-13) had higher GQs and locomotor, personal-social, and performance subscale quotients. The GQ was significantly different among the three age subgroups and significantly correlated with age. Compared with the non-epilepsy group, the epilepsy group had lower GQs and lower quotients for the locomotor, personal-social, speech, language, and eye-hand coordination subscales.

Conclusion: Children with AS in China experience severe neurodevelopmental deterioration. In addition to age, molecular subtypes and the onset of seizures may also correlate with these patients' intellectual development. The GMDS-C is an accurate tool that can assess the clinical characteristics of AS. The data of this study can be used as baseline data for clinical trials performed to evaluate drug development or other AS treatment development.
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http://dx.doi.org/10.3389/fpsyt.2022.886028DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9096167PMC
April 2022

Screening for Autism Spectrum Disorder in Toddlers During the 18- and 24-Month Well-Child Visits.

Front Psychiatry 2022 26;13:879625. Epub 2022 Apr 26.

Department of Child Healthcare, Children's Hospital of Fudan University, Shanghai, China.

Objective: Early screening contributes to the early detection of children with autism spectrum disorder (ASD). We conducted a longitudinal ASD screening study in a large community setting. The study was designed to investigate the diagnostic rate of ASD screening and determine the effectiveness of ASD screening model in a community-based sample.

Methods: We enrolled children who attended 18- and 24-month well-child care visits in Shanghai Xuhui District. Modified Checklist for Autism in Toddlers, Revised with Follow-up (M-CHAT-R/F) and Binomial Observation Test (BOT) were selected as screening instruments. Screen-positive children were referred to a tertiary diagnostic center for comprehensive ASD diagnostic evaluation. Screen-negative children received well-child checkups and follow-up every 3-6 months until age three and were referred if they were suspected of having ASD.

Results: A total of 11,190 toddlers were screened, and 36 screen-positive toddlers were diagnosed with ASD. The mean age at diagnosis for these children was 23.1 ± 4.55 months, diagnosed 20 months earlier than ASD children not screened. The diagnostic rate of ASD was 0.32% (95% CI: 0.23-0.45%) in this community-based sample. In addition, 12 screen-negative children were diagnosed with ASD during subsequent well-child visit and follow-up. The average diagnostic rate of ASD rose to 0.43% (95% CI: 0.32-0.57%) when toddlers were followed up to 3 years old. The positive predictive values (PPVs) of M-CHAT-R/F, M-CHAT-R high risk, and BOT for ASD were 0.31, 0.43, and 0.38 respectively.

Conclusion: Our findings provide reliable data for estimating the rate of ASD detection and identifying the validity of community-based screening model. M-CHAT-R/F combined with BOT can be an effective tool for early detection of ASD. This community-based screening model is worth replicating.
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http://dx.doi.org/10.3389/fpsyt.2022.879625DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9097214PMC
April 2022

Open Eyes Increase Neural Oscillation and Enhance Effective Brain Connectivity of the Default Mode Network: Resting-State Electroencephalogram Research.

Front Neurosci 2022 27;16:861247. Epub 2022 Apr 27.

Beijing Institute of Basic Medical Sciences, Beijing, China.

The default mode network (DMN) has a unique activity pattern in the resting brain. Studies on resting-state brain activity are helpful to identify various brain dynamic characteristics of patients with mental diseases and those of healthy people. The brain produces a series of changes in different eye states. However, the relationship between eye states and the DMN, which is closely related to the resting state, has not been widely examined. This study recruited 42 healthy students aged 17-22. Participants completed the Profile of Mood States questionnaire. Thereafter, the electroencephalogram data was collected with the patients' eyes open and closed. Changes in neural oscillation and the DMN's information transmission during different eye openness states were compared. The results showed that the neural oscillation activities of the parietal-occipital network such as the superior parietal lobule and precuneus were significantly enhanced in the eyes open state. In addition, the effective connectivity within the DMN was enhanced during opened eyes, especially from the left precuneus to the left posterior cingulate cortex, and this connectivity was negatively correlated with the Vigor-Activity mood state in the eyes open state. The activity of the DMN in the resting-state is regulated by eye states, which may relate to mood and emotional perception.
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http://dx.doi.org/10.3389/fnins.2022.861247DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9092973PMC
April 2022

Association between cine CMR-based radiomics signature and microvascular obstruction in patients with ST-segment elevation myocardial infarction.

J Thorac Dis 2022 Apr;14(4):969-978

Department of Radiology, Shenzhen People's Hospital (The Second Clinical College of Jinan University, The First Affiliated Hospital of Southern University of Science and Technology), Shenzhen, China.

Background: In the process of percutaneous coronary intervention (PCI), patients with ST-segment elevation myocardial infarction (STEMI) may receive large doses of the iodine contrast agent. Some adverse events may be aroused if the patients receive the gadolinium agents. We investigate the association between cine cardiac magnetic resonance (CMR)-based radiomics signature and microvascular obstruction (MVO) in patients with STEMI.

Methods: A total of 116 STEMI patients who received continuous CMR within 6 days after PCI were retrospectively included in this study. According to the late gadolinium enhancement (LGE) of CMR, the myocardial infarction (MI) was divided into with and without MVO. Radiomic features were extracted from cine CMR images and the least absolute shrinkage and selectionator operator (LASSO) algorithm was used for features selection and radiomic signatures construction. Binary logistic regression was used to assess association between radiomic signatures and MVO with adjusted for baseline clinical characteristics.

Results: Of 116 patients with STEMI, MI with MVO was found in 50 patients and MI without MVO was found in 66 patients. LASSO regression selected five radiomics features for radiomics signature construction. Logistic regression revealed that radiomics score, high sensitivity C-reactive protein (hs-CRP) and creatine phosphokinases (CPK) were independent risk factors for MVO with odds ratio (OR) of 4.41 (95% CI: 2.26-9.93), 1.018 (95% CI: 1.006-1.034) and 1.0007 (95% CI: 1.0004-1.0012), respectively. Area under curve (AUC) of receiver operating characteristic (ROC) of radiomics score to predict MVO was 0.75 (95% CI: 0.68-0.85).

Conclusions: Cine CMR-based radiomics signature was an independent predictive factor of MVO in patients with STEMI, which showed the potential of this contrast free radiomics signature to be an imaging biomarker for MVO.
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http://dx.doi.org/10.21037/jtd-21-1706DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9096300PMC
April 2022

Diagnosis of Atherosclerotic Plaques Using Vascular Endothelial Growth Factor Receptor-2 Targeting Antibody Nano-microbubble as Ultrasound Contrast Agent.

Comput Math Methods Med 2022 5;2022:6524592. Epub 2022 May 5.

Department of Ultrasonography, The Second Hospital of Hebei Medical University, Shijiazhuang, 050000 Hebei, China.

The atherosclerotic plaque is characterized by narrowing of blood vessels and reduced blood flow leading to the insufficient blood supply to the brain. The hemodynamic changes caused by arterial stenosis increase the shearing force of the fibrous cap on the surface of the plaque, thereby reducing the stability of the plaque. Unstable plaques are more likely to promote angiogenesis and increase the risk of patients with cerebrovascular diseases. A timely understanding of the formation and stability of the arterial plaque can guide in taking targeted measures for reducing the risk of acute stroke in patients. It has been confirmed that nano-microbubbles can enter these plaques through the gaps in the patient's vascular endothelial cells, thereby enhancing the acquisition of ultrasound information for plaque visualization. Therefore, we aim to investigate the diagnostic value of targeted nano-microbubbles for atherosclerotic plaques. This study constructed vascular endothelial growth factor receptor-2 (VEGFR-2) targeting antibody nano-microbubbles and compared its diagnostic value with that of blank nano-microbubbles for atherosclerotic plaques. Studies have found that VEGFR-2 targeting antibody nano-microbubbles can accurately detect the position of plaques. Its detection rate, sensitivity, and specificity for plaques are higher than those of blank nano-microbubbles. Similarly, the peak intensity and average transit time of VEGFR-2 targeting antibody nano-microbubbles were greater than those of blank nano-microbubbles. Therefore, we believe that the combination of VEGFR-2 antibody and nano-microbubbles can enhance the acquisition of ultrasound information on atherosclerotic plaque neovascularization, thereby improving the early diagnosis of unstable plaque.
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http://dx.doi.org/10.1155/2022/6524592DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9098277PMC
May 2022

m6A Reader Igf2bp1 Regulates the Inflammatory Responses of Microglia by Stabilizing and mRNAs.

Front Immunol 2022 29;13:872252. Epub 2022 Apr 29.

Center for Translational Neurodegeneration and Regenerative Therapy, Tongji Hospital Affiliated to Tongji University School of Medicine, Shanghai, China.

Microglia are brain resident cells that function as brain phagocytic macrophages. The inflammatory responses of microglia induced by pathologic insults are key regulators in the progression of various neurological disorders. Currently, little is known about how these responses are regulated intrinsically. Here, it is observed that LPS-activated microglia exhibit distinct N6-methyladenosine (m6A) methylation patterns that are positively correlated with the expression patterns of corresponding mRNAs. High-throughput analyses and molecular studies both identified Igf2bp1 as the most significantly regulated m6A modifiers in activated microglia. Perturbation of function approaches further indicated Igf2bp1 as a key mediator for LPS-induced m6A modification and microglial activation presumably enhancing the m6A methylation and stability of and mRNAs. Thus, our study provides a possible mechanism for the m6A methylation-mediated microglia regulation and identifies Igf2bp1 as a potential target for modulating the inflammatory responses of microglia.
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http://dx.doi.org/10.3389/fimmu.2022.872252DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100696PMC
May 2022
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