Publications by authors named "Yi Tan"

502 Publications

Behavioural and neurophysiological differences in working memory function of depressed patients and healthy controls.

J Affect Disord 2021 Aug 31;295:559-568. Epub 2021 Aug 31.

School of Psychiatry, University of New South Wales, Sydney, Australia; Faculty of Psychology and Neuroscience, Maastricht University, Maastricht, the Netherlands.

Objective: Major depressive disorder (MDD) is associated with deficits in working memory. Several cognitive subprocesses interact to produce working memory, including attention, encoding, maintenance and manipulation. We sought to clarify the contribution of functional deficits in these subprocesses in MDD by varying cognitive load during a working memory task.

Methods: 41 depressed participants and 41 age and gender-matched healthy controls performed the n-back working memory task at three levels of difficulty (0-, 1-, and 2-back) in a pregistered study. We assessed response times, accuracy, and event-related electroencephalography (EEG), including P2 and P3 amplitudes, and frontal theta power (4-8 Hz).

Results: MDD participants had prolonged response times and more positive frontal P3 amplitudes (i.e., Fz) relative to controls, mainly in the most difficult 2-back condition. Working memory accuracy, P2 amplitudes and frontal theta event-related synchronisation did not differ between groups at any level of task difficulty.

Conclusions: Depression is associated with generalized psychomotor slowing of working memory processes, and may involve compensatory hyperactivity in frontal and parietal regions.

Significance: These findings provide insights into MDD working memory deficits, indicating that depressed individuals dedicate greater levels of cortical processing and cognitive resources to achieve comparable working memory performance to controls.
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http://dx.doi.org/10.1016/j.jad.2021.08.083DOI Listing
August 2021

Two-photon fluorescent turn-on probes for highly efficient detection and profiling of thiols in live cells and tissues.

Biol Chem 2021 Sep 9. Epub 2021 Sep 9.

School of Pharmacy, Jinan University, 601 Huangpu Avenue West, Guangzhou510632, China.

Thiols are important units in amino acids such as cysteine and peptides like glutathione. Development of chemical sensors capable of precise detection of thiols is important in cancer diagnosis and therapy. We have developed novel two-photon fluorescent turn-on probes for selective detection of thiols. The probes displayed excellent sensitivity and low detection limits. The dual-purpose probes have been demonstrated to be suitable for simultaneous imaging and proteome profiling in live cells and tumor tissues. The unique turn-on design endows the probes with excellent selectivity toward thiols and , and can be further developed to support a thiol-quantification assay.
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http://dx.doi.org/10.1515/hsz-2021-0189DOI Listing
September 2021

Outpatient versus inpatient Foley catheter induction of labor in multiparas with unripe cervixes: A randomized trial.

Acta Obstet Gynecol Scand 2021 Aug 30. Epub 2021 Aug 30.

Department of Obstetrics and Gynecology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.

Introduction: Multiparous labor inductions are typically successful, and the process can be rapid, starting from a ripened cervix with a predictable response to amniotomy and oxytocin infusion. Outpatient Foley catheter labor induction in multiparas with unripe cervixes is a feasible option as the mechanical process of ripening is usually without significant uterine contractions and well tolerated. Labor contractions can be initiated by amniotomy and titrated oxytocin infusion in the hospital for well-timed births during working hours as night birth are associated with adverse events. We sought to evaluate outpatient compared with inpatient Foley catheter induction of labor in multiparas for births during working hours and maternal satisfaction.

Material And Methods: A randomized trial was conducted in the University of Malaya Medical Center. A total of 163 term multiparas (no dropouts) with unripe cervixes (Bishop score ≤5) scheduled for labor induction were randomized to outpatient or inpatient Foley catheter. Primary outcomes were delivery during "working hours" 08:00-18:00 h and maternal satisfaction on allocated care (assessed by 11-point visual numerical rating score 0-10, with higher score indicating more satisfied).

Clinical Trial Registration: ISRCTN13534944.

Results: Comparing outpatient and inpatient arms, delivery during working hours were 54/82 (65.9%) vs. 48/81 (59.3%) (relative risk 1.1, 95% CI 0.9-1.4, p = 0.421) and median maternal satisfaction visual numerical rating score was 9 (interquartile range 9-9) vs. 9 (interquartile range 8-9, p = 0.134), repectively. Duration of hospital stay and membrane rupture to delivery interval were significantly shorter in the outpatient arm: 35.8 ± 20.2 vs. 45.2 ± 16.2 h (p = 0.001) and 4.1 ± 2.9 vs. 5.3 ± 3.6 h (p = 0.020), respectively. Other maternal and neonatal secondary outcomes were not significantly different.

Conclusions: The trial failed to demonstrate the anticipated increase in births during working hours with outpatient compared with inpatient induction of labor with Foley catheter in parous women with an unripe cervix. Hospital stay and membrane rupture to delivery interval were significantly shortened in the outpatient group. The rate of maternal satisfaction was high in both groups and no significant differences were found.
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http://dx.doi.org/10.1111/aogs.14247DOI Listing
August 2021

Myeloproliferative neoplasms: Classifications and an approach to diagnosis.

Aust J Gen Pract 2021 09;50(9):622-627

MBBS, MMed (Clin Epi), FRACP, FRCPA, Clinical and Laboratory Haematologist, Nepean Hospital, Kingswood, NSW.

Background: Myeloproliferative neoplasms (MPNs) are a group of illnesses that share a tendency to transform into myelofibrosis and acute leukaemias. Their initial presenting symptoms may be non-specific, which can make diagnosis difficult without a structured approach.

Objective: The aim of the article is to provide general practitioners (GPs) insight into the classification and clinical approach to diagnosis of MPNs.

Discussion: An elevated peripheral blood cell count is often among the first presenting features of an MPN. Although MPNs are rare illnesses, the GP is well placed to identify suspicious features and initiate investigations and referral. It is therefore important for GPs to have an approach to differentiating between reactive and neoplastic causes of elevated blood cell counts.
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http://dx.doi.org/10.31128/AJGP-03-21-5868DOI Listing
September 2021

Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes Improve Ovarian Function and Proliferation of Premature Ovarian Insufficiency by Regulating the Hippo Signaling Pathway.

Front Endocrinol (Lausanne) 2021 12;12:711902. Epub 2021 Aug 12.

Department of Obstetrics and Gynecology, The Second Hospital of Hebei Medical University, Shijiazhuang, China.

Background: Premature ovarian insufficiency (POI) is associated with severe physical damage and psychological burden on women. Transplantation of exosomes is an encouraging regenerative medicine method, which has the potential for restoring ovarian functions on POI with high efficiency. This study aims at evaluating the therapeutic efficacy of human umbilical cord mesenchymal stem cell-derived exosomes (hUCMSC-Exos) on ovarian dysfunction of POI and the role of Hippo pathway in this exosome-mediated treatment.

Methods: POI mice models were established through intraperitoneal injection of cyclophosphamide. Subsequently, transplantation of hUCMSC-Exos was conducted to administer POI mice. Ovaries and plasma of these mice models were harvested after two weeks of treatment. Ovarian morphology and follicle number were assessed by hematoxylin and eosin staining. Moreover, ELISA was used to detect hormone levels, which are related to ovarian function in serum. To assess the recovery of reproductive ability, we recorded the rate of pregnancy, the amount of offspring, and the time of birth in different groups. To explore the underlying mechanisms of exosome-mediated treatment for ovarian function recovery, the proliferation of ovarian cells was detected by immunohistochemistry and immunofluorescence staining. Additionally, we conducted EdU and CCK-8 assays to assess the proliferative ability of ovarian granulosa cells (GCs) that were cultured . Western blot analysis was conducted to estimate the proteins levels of Hippo- and proliferation-associated molecules .

Results: After transplantation of hUCMSC-Exos, the ovarian function-related hormone levels and the number of ovarian follicles returned to nearly normal degrees. Meanwhile, there was a significant improvement in reproductive outcomes after exosomal treatment. Furthermore, the improvement of ovarian function and proliferation was associated with the regulation of Hippo pathway. , co-culture with exosomes significantly elevated the proliferation of ovarian GCs by regulating Hippo pathway. However, the positive effects on the proliferation of GCs were significantly depressed when key Hippo pathway molecule was inhibited.

Conclusion: This study suggested that hUCMSC-Exos promoted ovarian functions and proliferation by regulating the Hippo pathway. Therefore, exosomal transplantation could be a promising and efficient clinical therapy for POI in the near future.
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http://dx.doi.org/10.3389/fendo.2021.711902DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8397419PMC
August 2021

Validation of pharmaceutical pictograms among older adults with limited English proficiency.

Patient Educ Couns 2021 Jul 10. Epub 2021 Jul 10.

Department of Pharmacy, Children's Hospital of Eastern Ontario, Canada Department of Pharmacy, Children's Hospital of Eastern Ontario, Canada.

Objective: Pictograms on prescription medication labels enhance medication literacy and medication adherence. However, pictograms need to be contextually validated. We assessed the validity of 52 International Pharmaceutical Federation pictograms among 250 older Singaporeans with limited English proficiency.

Methods: Participants were randomly assigned 11 pictograms each. For each pictogram, participants were first asked its intended meaning. Then, they were told the intended meaning and asked to rate how well the pictogram represented the meaning, on a scale of 1-7. Pictograms were classified as valid (≥66% participants assigned the pictogram interpreted its intended meaning correctly [transparency criterion] and ≥85% participants rated its representativeness as ≥5 [translucency criterion]), partially valid (only transparency criterion was fulfilled) or not valid. Open-ended questions gathered feedback to improve pictograms.

Results: 14 pictograms (26.9%) achieved validity and 6 pictograms (11.5%) achieved partial validity. A greater proportion of pictograms for dose and route of administration, and dosage frequency achieved validity or partial validity versus those depicting precautions, indications or side effects.

Conclusion: Majority (61.5%) of the assessed pictograms did not achieve validity or partial validity, highlighting the importance of contextual validation.

Practice Implications: Low pictogram comprehension emphasizes the importance of facilitating pictogram understanding during medication counseling.
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http://dx.doi.org/10.1016/j.pec.2021.07.015DOI Listing
July 2021

The Combination of Age, International Standardized Ratio, Albumin and γ-Glutamyl Transpeptidase (AIAG), Tumor Size and Alpha Fetoprotein (AFP) Stage as the Prognostic Model for Hepatitis B-Related Hepatocellular Carcinoma.

Int J Gen Med 2021 10;14:4291-4301. Epub 2021 Aug 10.

Department of Hepatobiliary Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, 233000, Anhui, People's Republic of China.

Background: Advanced liver fibrosis can lead to cirrhosis, portal hypertension and liver failure. Besides, advanced liver fibrosis and cirrhosis are the major risk factors for hepatocellular carcinoma (HCC). Almost all patients with HCC also have liver cirrhosis. This study aims to predict the survival rate of hepatitis B-related hepatocellular carcinoma (HCC) by age, international standardized ratio, albumin and γ-glutamyl transpeptidase (AIAG), an indicator measuring the degree of cirrhosis.

Methods: A total of 501 hepatitis B-related HCC patients experiencing radical surgery were analyzed, retrospectively. General data about demographics and labs were collected at the date of diagnosis to calculate AIAG [age, international standardized ratio (INR), albumin and gamma-glutamyl transferase (GGT)]. The Kaplan-Meier curves and Cox analysis were used to evaluate overall survival (OS) and recurrence-free survival (RFS). The C-index was calculated in R software (version 4.0.3) to evaluate the accuracy of the prognostic model.

Results: During a median follow-up period of 30 months, 31.1% (156/501) of the patients died, and 34.3% (172/501) experienced the recurrence of HCC. Compared with patients with lower AIAG score, patients with higher AIAG score had higher Child-Pugh grade and were at higher Barcelona Clinic Liver Cancer (BCLC) stage (both <0.05). Multivariate analysis suggested that GGT, alpha fetoprotein (AFP), tumor size, BCLC stage and AIAG grade were independent predictors of OS and RFS. Furthermore, the combined use of tumor size, AFP and AIAG stage could predict survival significantly better (C-index=0.710, 95% CI: 0.669-0.751) than BCLC stage.

Conclusion: AIAG is significantly associated with survival of HCC patients, and provides additional prognostic information for patients with HCC. Our findings suggest that the combination of AIAG, tumor size and AFP stage has a better predictive value for the prognosis of patients with hepatitis B-related hepatocellular carcinoma. However, it is necessary for more external evidences to determine clinical utility.
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http://dx.doi.org/10.2147/IJGM.S323293DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8364385PMC
August 2021

Combination of Ferulic Acid, Ligustrazine and Tetrahydropalmatine attenuates Epithelial-mesenchymal Transformation Wnt/β-catenin Pathway in Endometriosis.

Int J Biol Sci 2021 11;17(10):2449-2460. Epub 2021 Jun 11.

College of Pharmaceutical Sciences & Chinese Medicine, Southwest University, Chongqing, China.

Previously the potential therapeutic action of ferulic acid, ligustrazine and tetrahydropalmatine (FLT) are discovered with unclear mechanism in rat autograft endometriosis. However, the effect of FLT on endometrial cells and allograft endometriosis is still unclear. This study is designed to elucidate the influence of FLT on epithelial-mesenchymal transformation in allograft endometriosis and endometrium cells. , fluorescent xenogeneic endometriosis model was established. , invasion and metastasis were analyzed after treating FLT. Epithelial-mesenchymal transformation and Wnt/β-catenin pathway were inspected and . Activator or inhibitor of Wnt/β-catenin signaling was performed to inspect mechanism of epithelial-mesenchymal transformation. , FLT not only decreased fluorescent intensity and volume of ectopic lesion, but also ameliorated pathological morphology. E and PROG levels in serum were reduced by FLT. In endometrial cells, FLT significantly inhibited the invasion and metastasis. Meantime, epithelial-mesenchymal transformation was reversed, accompanied by suppression of Wnt/β-catenin pathway. In-depth study, activation of Wnt/β-catenin pathway lead to promotion of epithelial-mesenchymal transformation, which was reversed by FLT. FLT prevented fluorescent allograft endometriosis and endometrium cells, which was related to suppress epithelial-mesenchymal transformation through inactivating Wnt/β-catenin pathway. The findings disclose molecular mechanism of epithelial-mesenchymal transformation in endometriosis by FLT, and contribute to further application.
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http://dx.doi.org/10.7150/ijbs.60167DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8315018PMC
June 2021

Hypoxia-induced alteration of RNA modifications in the mouse testis and sperm.

Biol Reprod 2021 Jul 22. Epub 2021 Jul 22.

Laboratory Animal Center, Chongqing Medical University, Yixueyuan Road 1, Yuzhong District, Chongqing, 400016, P. R. China.

Hypobaric hypoxia as an extreme environment in a plateau may have deleterious effects on human health. Studies have indicated that rush entry into a plateau may reduce male fertility and manifest in decreased sperm counts and weakened sperm motility. RNA modifications are sensitive to environmental changes and have recently emerged as novel post-transcriptional regulators in male spermatogenesis and intergenerational epigenetic inheritance. In the present study, we generated a mouse hypoxia model simulating the environment of 5500 meters in altitude for 35 days, which led to compromised spermatogenesis, decreased sperm counts, and an increased sperm deformation rate. Using this hypoxia model, we further applied our recently developed high-throughput RNA modification quantification platform based on LC-MS/MS, which exhibited the capacity to simultaneously examine 25 types of RNA modifications. Our results revealed an altered sperm RNA modifications signature in the testis (6 types) and mature sperm (11 types) under the hypoxia model, with 4 types showing overlap (Am, Gm, m7G, and m22G). Our data first drew the signature of RNA modification profiles and comprehensively analyzed the alteration of RNA modification levels in mouse testis and sperm under a mouse hypoxia model. These data may be highly related to human conditions under a similar hypoxia environment.
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http://dx.doi.org/10.1093/biolre/ioab142DOI Listing
July 2021

Electron Transfer Quenching of Rhodamine 6G by -Methylpyrrole Is an Unproductive Process in the Photocatalytic Heterobiaryl Cross-Coupling Reaction.

J Phys Chem B 2021 08 21;125(30):8550-8557. Epub 2021 Jul 21.

Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, 636371, Singapore.

In the heterobiaryl cross-coupling reaction between aryl halides (Ar-X) and -methylpyrrole (-MP) catalyzed by rhodamine 6G (Rh6G) under irradiation with visible light, a highly active and long-lived (millisecond time range) rhodamine 6G radical (Rh6G) is formed upon electron transfer from ,-diisopropylethylamine (DIPEA) to Rh6G. In this study, we utilized steady-state and time-resolved spectroscopy techniques to demonstrate the existence of another electron-transfer process occurring from the relatively electron-rich -MP to photoexcited Rh6G that was neglected in the previous reports. In this case, the radical Rh6G formed is short-lived and undergoes rapid recombination (nanosecond time-range), rendering it ineffective in reducing Ar-X to aryl radicals Ar that can subsequently be trapped by -MP. This is further demonstrated two model reactions involving 4'-bromoacetophenone and 1,3,5-tribromobenzene with insignificant product yields after visible-light irradiation in the absence of DIPEA. The unproductive quenching of photoexcited Rh6G by -MP leads to a lower concentration of photocatalyst available for competitive charge transfer with DIPEA and hence decreases the efficiency of the cross-coupling reaction.
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http://dx.doi.org/10.1021/acs.jpcb.1c04536DOI Listing
August 2021

Cross-sectional study of prevalence and risk factors, and a cost-effectiveness evaluation of screening and preventive treatment strategies for latent tuberculosis among migrants in Singapore.

BMJ Open 2021 07 15;11(7):e050629. Epub 2021 Jul 15.

Saw Swee Hock School of Public Health, National University of Singapore and National University Health System, Singapore.

Objectives: WHO recommends that low burden countries consider systematic screening and treatment of latent tuberculosis infection (LTBI) in migrants from high incidence countries. We aimed to determine LTBI prevalence and risk factors and evaluate cost-effectiveness of screening and treating LTBI in migrants to Singapore from a government payer perspective.

Design: Cross-sectional study and cost-effectiveness analysis.

Setting: Migrants in Singapore.

Participants: 3618 migrants who were between 20 and 50 years old, have not worked in Singapore previously and stayed in Singapore for less than a year were recruited.

Primary And Secondary Outcome Measures: Costs, quality-adjusted life-years (QALYs), threshold length of stay, incremental cost-effectiveness ratios (ICERs), cost per active TB case averted.

Results: Of 3584 migrants surveyed, 20.4% had positive interferon-gamma release assay (IGRA) results, with the highest positivity in Filipinos (33.2%). Higher LTBI prevalence was significantly associated with age, marital status and past TB exposure. The cost-effectiveness model projected an ICER of S$57 116 per QALY and S$12 422 per active TB case averted for screening and treating LTBI with 3 months once weekly isoniazid and rifapentine combination regimen treatment compared with no screening over a 50-year time horizon. ICER was most sensitive to the cohort's length of stay in Singapore, yearly disease progression rates from LTBI to active TB, followed by the cost of IGRA testing.

Conclusions: For LTBI screening and treatment of migrants to be cost-effective, migrants from high burden countries would have to stay in Singapore for ~50 years. Risk-stratified approaches based on projected length of stay and country of origin and/or age group can be considered.
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http://dx.doi.org/10.1136/bmjopen-2021-050629DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8286773PMC
July 2021

Combination of ferulic acid, ligustrazine and tetrahydropalmatine inhibits invasion and metastasis through MMP/TIMP signaling in endometriosis.

PeerJ 2021 28;9:e11664. Epub 2021 Jun 28.

College of Pharmaceutical Sciences & Chinese Medicine, Southwest University, Chongqing, China.

Background: The design of the combination of ferulic acid, ligustrazine and tetrahydropalmatine (FLT) is inspired by the Chinese herbal prescription Foshou San. Previous work has shown that FLT inhibited endometriosis growth in rat autograft models. However, the mechanism behind this is unclear. MMP/TIMP signaling is considered as the vital pathway of metastasis and invasion in endometriosis. In this study, we aim to disclose effects of FLT on MMP/TIMP signaling in invasion and metastasis during endometrial cells and xenograft endometriosis.

Methods: In vivo, effect of FLT on endometriosis was evaluated in a xenogeneic mice model. In vitro, cell viability assay was performed with an IC50 measurement of FLT in hEM15A and HEC1-B cells. The effect of FLT on invasion and metastasis was analyzed in scratch wound and transwell assay. Gene and protein expression of MMP/TIMP signaling were detected by qPCR and Western blotting.

Results: In xenograft endometriosis, FLT reduced ectopic volume without effect on weight. FLT inhibitory effects on cell growth exhibited a dose-dependent manner in hEM15A and HEC1-B cells. IC50s of FLT in hEM15A cells were 839.30 ± 121.11 or 483.53 ±156.91 μg·ml after the treatment for 24 or 48 h, respectively. In HEC1-B cells, IC50 values of 24 or 48 h were 625.20 ± 59.52 or 250.30 ± 68.12 μg·ml. In addition, FLT significantly inhibited invasion and metastasis in scratch wound and transwell assay. Furthermore, FLT inactivated MMP/TIMP signaling with decreasing expression of MMP-2/9, and an enhancing expression of TIMP-1.

Conclusions: MMP/TIMP inactivation is a reasonable explanation for the inhibition of FLT on invasion and metastasis in endometriosis. This result reveals a potential mechanism on the role of FLT in endometriosis and may benefit for its further application.
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http://dx.doi.org/10.7717/peerj.11664DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8247703PMC
June 2021

Implementation and data processing of a five-channel microwave interferometer with high temporal resolution and low noise on Sino-UNIted Spherical Tokamak.

Rev Sci Instrum 2021 Apr;92(4):043538

Department of Engineering Physics, Tsinghua University, Beijing 100084, People's Republic of China.

A five-channel microwave interferometer with high temporal resolution and high phase resolution has been developed for measuring electron density profiles and fluctuations on the Sino-UNIted Spherical Tokamak. A correction algorithm, based on the low signal amplitude detection, is proposed to eliminate the fringe jump errors. The correction algorithm achieves an accuracy of 92%. The density profile is reconstructed through the Park-matrix method, with the geometry of magnetic surfaces calculated by the equilibrium fitting. The reconstructed density profiles for discharges with supersonic molecular beam injection are in good agreement with the results of another 94 GHz single-channel horizontal interferometer and the Langmuir probes. The temporal resolution of the system is 0.5 µs and the line-integrated electron density resolution is 1 × 10 m, which benefits from the single sideband modulation technique. Therefore, the multichannel interferometer system is capable of studying the details of the high-frequency (up to 200 kHz) density fluctuation such as that in the minor disruption event.
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http://dx.doi.org/10.1063/5.0043667DOI Listing
April 2021

Modular bolometric/soft x-ray diagnostic in Sino-UNIted Spherical Tokamak.

Rev Sci Instrum 2021 Apr;92(4):043540

Department of Engineering Physics: Tsinghua University, Beijing 100084, China.

A bolometer/soft x-ray diagnostic based on modular absolute extreme ultraviolet photodiodes has been built to obtain the local emission profile of a poloidal cross section in Sino-UNIted Spherical Tokamak (SUNIST). The system consists of seven photodiode arrays of 112 channels located around the low field side of a poloidal cross section. All arrays are mounted into 81 × 36 × 33 mm modules, which could be installed flexibly on the wall of the vacuum vessel. Tungsten shields with adjustable pinholes restrict the radiation flux. Compact flexible printed circuits with multilayer copper polygon poured on both sides are designed to transmit signals with electronic noise, which is reduced to less than 1/10 before. A two-stage transimpedance amplifier array of 700 kHz bandwidth is fixed on the atmosphere side of a vacuum electrical feedthrough and is powered by battery packs. A synchronized switch shuts down the circuit to reduce the heat production and increase the battery life. A line-integrated radiation emission profile with 2 cm spatial resolution and temporal resolution up to 2 µs is obtained, and the local emission profile of SUNIST is reconstructed with the help of a newly developed tomography scheme for movable detector modules.
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http://dx.doi.org/10.1063/5.0043678DOI Listing
April 2021

Development of a thin high-frequency and high-precision magnetic probe array in Sino-United Spherical Tokamak.

Rev Sci Instrum 2021 May;92(5):053518

Department of Engineering Physics, Tsinghua University, Beijing 10084, People's Republic of China.

Since the major/minor radius of the Sino-United Spherical Tokamak (SUNIST) is 0.3/0.23 m, respectively, the space left for magnetic diagnostics in the high field side (HFS) is quite limited. At the same time, a good signal-to-noise ratio and a high-frequency response (up to 1 MHz) are required for equilibrium reconstruction (ER) and Alfven eigenmode studies. Such a magnetic probe array must be extremely thin and tightly close to the central column, not exceeding the inner limiter and leaving the aspect ratio of the spherical tokamak unchanged. Therefore, the turn number and the shape of windings should be highly optimized to enable both a high-frequency response and an enough effective area. A 32-channel magnetic probe array fulfilling these requirements on the central column is designed, calibrated, and installed in the SUNIST. The array consists of 16 probes. Each of them consists of two perpendicular windings, which can measure toroidal and poloidal magnetic fields simultaneously. The effective area and frequency response of each probe are calibrated using a Helmholtz coil and an LCR bridge based on an equivalent probe-and-cable circuit model. After that, an expression of the magnetic diagnostic response to the field coil currents is used to calibrate the installation error. With the full coverage of magnetic probes in the poloidal direction, more reliable ER can be obtained, and the features of magnetohydrodynamic activities in the HFS can be studied.
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http://dx.doi.org/10.1063/5.0043631DOI Listing
May 2021

SIMULATION AND MEASUREMENT OF EXTERNAL ELECTROMAGNETIC ENVIRONMENT OF TOKAMAK DEVICE.

Radiat Prot Dosimetry 2021 Aug;194(4):187-195

Department of Engineering Physics, Tsinghua University, Beijing 100084, China.

Magnetic confinement nuclear fusion is an important way to realize controllable nuclear fusion. Due to the large current and complicated coil arrangement, there is a complicated electromagnetic environment around the fusion device. In this paper, the B-dot sensor is used to measure the magnetic field, the D-dot sensor is used to measure the electric field, the MAXWELL electromagnetic simulation software is used to simulate the electromagnetic field strength; the simulation and measurement of the spherical Tokamak SUNIST device and the measurement of MARX generator are carried out, then we give corresponding electromagnetic protection suggestions.
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http://dx.doi.org/10.1093/rpd/ncab101DOI Listing
August 2021

BIM and Data-Driven Predictive Analysis of Optimum Thermal Comfort for Indoor Environment.

Sensors (Basel) 2021 Jun 27;21(13). Epub 2021 Jun 27.

Department of Civil and Environmental Engineering, Hong Kong University of Science and Technology, Hong Kong, China.

Mechanical ventilation comprises a significant proportion of the total energy consumed in buildings. Sufficient natural ventilation in buildings is critical in reducing the energy consumption of mechanical ventilation while maintaining a comfortable indoor environment for occupants. In this paper, a new computerized framework based on building information modelling (BIM) and machine learning data-driven models is presented to analyze the optimum thermal comfort for indoor environments with the effect of natural ventilation. BIM provides geometrical and semantic information of the built environment, which are leveraged for setting the computational domain and boundary conditions of computational fluid dynamics (CFD) simulation. CFD modelling is conducted to obtain the flow field and temperature distribution, the results of which determine the thermal comfort index in a ventilated environment. BIM-CFD provides spatial data, boundary conditions, indoor environmental parameters, and the thermal comfort index for machine learning to construct robust data-driven models to empower the predictive analysis. In the neural network, the adjacency matrix in the field of graph theory is used to represent the spatial features (such as zone adjacency and connectivity) and incorporate the potential impact of interzonal airflow in thermal comfort analysis. The results of a case study indicate that utilizing natural ventilation can save cooling power consumption, but it may not be sufficient to fulfil all the thermal comfort criteria. The performance of natural ventilation at different seasons should be considered to identify the period when both air conditioning energy use and indoor thermal comfort are achieved. With the proposed new framework, thermal comfort prediction can be examined more efficiently to study different design options, operating scenarios, and changeover strategies between various ventilation modes, such as better spatial HVAC system designs, specific room-based real-time HVAC control, and other potential applications to maximize indoor thermal comfort.
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http://dx.doi.org/10.3390/s21134401DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8271837PMC
June 2021

BZD9L1 sirtuin inhibitor: Identification of key molecular targets and their biological functions in HCT 116 colorectal cancer cells.

Life Sci 2021 Jun 24:119747. Epub 2021 Jun 24.

Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Penang 11800, Malaysia. Electronic address:

BZD9L1 was previously described as a SIRT1/2 inhibitor with anti-cancer activities in colorectal cancer (CRC), either as a standalone chemotherapy or in combination with 5-fluorouracil. BZD9L1 was reported to induce apoptosis in CRC cells; however, the network of intracellular pathways and crosstalk between molecular players mediated by BZD9L1 is not fully understood. This study aimed to uncover the mechanisms involved in BZD9L1-mediated cytotoxicity based on previous and new findings for the prediction and identification of related pathways and key molecular players. BZD9L1-regulated candidate targets (RCTs) were identified using a range of molecular, cell-based and biochemical techniques on the HCT 116 cell line. BZD9L1 regulated major cancer pathways including Notch, p53, cell cycle, NFκB, Myc/MAX, and MAPK/ERK signalling pathways. BZD9L1 also induced reactive oxygen species (ROS), regulated apoptosis-related proteins, and altered cell polarity and adhesion profiles. In silico analyses revealed that most RCTs were interconnected, and were involved in the modulation of catalytic activity, metabolism and transcription regulation, response to cytokines, and apoptosis signalling pathways. These RCTs were implicated in p53-dependent apoptosis pathway. This study provides the first assessment of possible associations of molecular players underlying the cytotoxic activity of BZD9L1, and establishes the links between RCTs and apoptosis through the p53 pathway.
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http://dx.doi.org/10.1016/j.lfs.2021.119747DOI Listing
June 2021

BZD9L1 sirtuin inhibitor: Identification of key molecular targets and their biological functions in HCT 116 colorectal cancer cells.

Life Sci 2021 Jun 24:119747. Epub 2021 Jun 24.

Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Penang 11800, Malaysia. Electronic address:

BZD9L1 was previously described as a SIRT1/2 inhibitor with anti-cancer activities in colorectal cancer (CRC), either as a standalone chemotherapy or in combination with 5-fluorouracil. BZD9L1 was reported to induce apoptosis in CRC cells; however, the network of intracellular pathways and crosstalk between molecular players mediated by BZD9L1 is not fully understood. This study aimed to uncover the mechanisms involved in BZD9L1-mediated cytotoxicity based on previous and new findings for the prediction and identification of related pathways and key molecular players. BZD9L1-regulated candidate targets (RCTs) were identified using a range of molecular, cell-based and biochemical techniques on the HCT 116 cell line. BZD9L1 regulated major cancer pathways including Notch, p53, cell cycle, NFκB, Myc/MAX, and MAPK/ERK signalling pathways. BZD9L1 also induced reactive oxygen species (ROS), regulated apoptosis-related proteins, and altered cell polarity and adhesion profiles. In silico analyses revealed that most RCTs were interconnected, and were involved in the modulation of catalytic activity, metabolism and transcription regulation, response to cytokines, and apoptosis signalling pathways. These RCTs were implicated in p53-dependent apoptosis pathway. This study provides the first assessment of possible associations of molecular players underlying the cytotoxic activity of BZD9L1, and establishes the links between RCTs and apoptosis through the p53 pathway.
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http://dx.doi.org/10.1016/j.lfs.2021.119747DOI Listing
June 2021

microRNA-17 is a tumor suppressor in oral squamous cell carcinoma and is repressed by LSD1.

Oral Dis 2021 Jun 21. Epub 2021 Jun 21.

Department of Stomatology, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou, China.

Objective: The effects of epigenetic modifiers have been uncovered on cellular reprogramming and, specifically, on sustaining characteristics of cancer stem cells. We here aim to investigate whether lysine-specific demethylase 1 (LSD1) affects the development of oral squamous cell carcinoma (OSCC) by sustaining the cancer stem cells from OSCC (OSCSCs).

Methods: RT-qPCR detection was firstly conducted to screen out research gene by determining differential expression of histone demethylases and methylases in identified OSCSCs. Then, microarray analysis was carried out in cells with poor expression of LSD1.

Results: OSCSCs expressed high levels of LSD1, and LSD1 inhibition reduced cell viability, migration, invasion, and sphere formation of OSCSCs. Later mechanistic studies suggested that LSD1 inhibited microRNA (miR)-17 expression through histone demethylation. miR-17 bound to KPNA2, and LSD1 downstream genes were mainly enriched in the PI3K/AKT pathway. Importantly, miR-17 inhibitor reversed the inhibitory effect of si-LSD1 on cell activity, while si-KPNA2 abolished the promotive effect of miR-17 inhibitor on cell activity both in vitro and in vivo.

Conclusion: Overall, LSD1 functions as a cancer stem cell supporter in OSCC by catalyzing demethylation of miR-17 and activating the downstream KPNA2/PI3K/AKT pathway, which contributes to understanding of the mechanisms associated with epigenetic regulation in OSCC.
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http://dx.doi.org/10.1111/odi.13944DOI Listing
June 2021

Enhanced electrochemical properties of Ni-rich layered cathode materials via Mg and Tico-doping for lithium-ion batteries.

J Colloid Interface Sci 2021 Nov 29;601:853-862. Epub 2021 May 29.

College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou 434023, PR China. Electronic address:

To optimize the electrochemical performance of Ni-rich cathode materials, the 0.005 mol of Mg and 0.005 mol of Tico-doping LiNiCoMnO composite powders, labeled as NCM-11, are successfully prepared by being calcinated at 750 °C for 15 h following by an appropriate post-treatment, which are confirmed by XRD, EDS and XPS. The results suggest that NCM-11 presents a well-ordered layered structure with a low Li/Ni mixing degree of 1.46% and Mg and Ti ions are uniformly distributed across the lattice. The cell assembled with NCM-11 can deliver an initial discharge specific capacity of 194.2 mAh g and retain a discharge specific capacity of 163.0 mAh g after 100cycles at 2.0C at 25 °C. Furthermore, it still maintains a discharge specific capacity of 166.7 mAh g after 100cycles at 2.0C at 60 °C. More importantly, it also exhibits a higher discharge specific capacity of about 150.7 mAh g even at 5.0C. Those superior electrochemical performance can be mainly ascribed to the synergistic effect of Mg and Tico-doping, in which Mg ions can occupy the Li layer to act as pillar ions and Ti ions can occupy the transition metal ions layer to enlarge the interplane spacing. Thus, the heterovalent cations co-doping strategy can be considered as a simple and practical method to improve the electrochemical performance of Ni-rich layered cathode materials for lithium-ion batteries.
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http://dx.doi.org/10.1016/j.jcis.2021.05.167DOI Listing
November 2021

Novel Optineurin Frameshift Insertion in a Family With Frontotemporal Dementia and Parkinsonism Without Amyotrophic Lateral Sclerosis.

Front Neurol 2021 19;12:645913. Epub 2021 May 19.

Department of Neurology, National Neuroscience Institute, Singapore, Singapore.

Frontotemporal Dementia (FTD) is a common cause of Young Onset Dementia and has diverse clinical manifestations involving behavior, executive function, language and motor function, including parkinsonism. Up to 50% of FTD patients report a positive family history, supporting a strong genetic basis, particularly in cases with both FTD and amyotrophic lateral sclerosis (FTD-ALS). Mutations in three genes are associated with the majority of familial FTD (fFTD) cases - microtubule associated protein tau gene (), granulin precursor (), and hexanucleotide repeat expansions in chromosome 9 open reading frame 72- SMCR8complex subunit (C9orf72) while mutations in other genes such as optineurin () have rarely been reported. Mutations in have been reported mostly in familial and sporadic cases of ALS, or in rare cases of FTD-ALS, but not in association with pure or predominant FTD and/or parkinsonian phenotype. Here, we report for the first time, a family from the Philippines with four members harboring a novel frameshift insertion at (Chr 10:13166090 G>GA) p.Lys328GluTer11, three of whom presented with FTD-related phenotypes. Additionally, one sibling heterozygous for the frameshift insertion had a predominantly parkinsonian phenotype resembling corticobasal syndrome, but it remains to be determined if this phenotype is related to the frameshift insertion. Notably, none of the affected members showed any evidence of motor neuron disease or ALS at the time of writing, both clinically and on electrophysiological testing, expanding the phenotypic spectrum of mutations. Close follow-up of mutation carriers for the development of new clinical features and wider investigation of additional family members with further genetic analyses will be conducted to investigate the possibility of other genetic modifiers in this family which could explain phenotypic heterogeneity.
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http://dx.doi.org/10.3389/fneur.2021.645913DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170397PMC
May 2021

Cardiac metallothionein overexpression rescues diabetic cardiomyopathy in Akt2-knockout mice.

J Cell Mol Med 2021 Jul 30;25(14):6828-6840. Epub 2021 May 30.

Department of Pediatrics, Pediatric Research Institute, University of Louisville School of Medicine, Louisville, KY, USA.

To efficiently prevent diabetic cardiomyopathy (DCM), we have explored and confirmed that metallothionein (MT) prevents DCM by attenuating oxidative stress, and increasing expression of proteins associated with glucose metabolism. To determine whether Akt2 expression is critical to MT prevention of DCM, mice with either global Akt2 gene deletion (Akt2-KO), or cardiomyocyte-specific overexpressing MT gene (MT-TG) or both combined (MT-TG/Akt2-KO) were used. Akt2-KO mice exhibited symptoms of DCM (cardiac remodelling and dysfunction), and reduced expression of glycogen and glucose metabolism-related proteins, despite an increase in total Akt (t-Akt) phosphorylation. Cardiac MT overexpression in MT-TG/Akt2-KO mice prevented DCM and restored glucose metabolism-related proteins expression and baseline t-Akt phosphorylation. Furthermore, phosphorylation of ERK1/2 increased in the heart of MT-TG/Akt2-KO mice, compared with Akt2-KO mice. As ERK1/2 has been implicated in the regulation of glucose transport and metabolism this increase could potentially underlie MT protective effect in MT-TG/Akt2-KO mice. Therefore, these results show that although our previous work has shown that MT preserving Akt2 activity is sufficient to prevent DCM, in the absence of Akt2 MT may stimulate alternative or downstream pathways protecting from DCM in a type 2 model of diabetes, and that this protection may be associated with the ERK activation pathway.
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http://dx.doi.org/10.1111/jcmm.16687DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8278119PMC
July 2021

A novel therapeutic approach for inflammatory bowel disease by exosomes derived from human umbilical cord mesenchymal stem cells to repair intestinal barrier via TSG-6.

Stem Cell Res Ther 2021 05 29;12(1):315. Epub 2021 May 29.

Department of Gastroenterology, The Second Hospital of Hebei Medical University, No. 80 Huanghe Road, Yuhua District, Shijiazhuang, 050000, Hebei, China.

Background: Exosomes as the main therapeutic vectors of mesenchymal stem cells (MSC) for inflammatory bowel disease (IBD) treatment and its mechanism remain unexplored. Tumor necrosis factor-α stimulated gene 6 (TSG-6) is a glycoprotein secreted by MSC with the capacities of tissue repair and immune regulation. This study aimed to explore whether TSG-6 is a potential molecular target of exosomes derived from MSCs (MSCs-Exo) exerting its therapeutic effect against colon inflammation and repairing mucosal tissue.

Methods: Two separate dextran sulfate sodium (DSS) and 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced IBD mouse models were intraperitoneally administered MSCs-Exo extracted from human umbilical cord MSC (hUC-MSC) culture supernatant. Effects of MSCs-Exo on intestinal inflammation, colon barrier function, and proportion of T cells were investigated. We explored the effects of MSCs-Exo on the intestinal barrier and immune response with TSG-6 knockdown. Moreover, recombinant human TSG-6 (rhTSG-6) was administered exogenously and colon inflammation severity in mice was evaluated.

Results: Intraperitoneal injection of MSCs-Exo significantly ameliorated IBD symptoms and reduced mortality rate. The protective effect of MSCs-Exo on intestinal barrier was demonstrated evidenced by the loss of goblet cells and intestinal mucosa permeability, thereby improving the destruction of tight junctions (TJ) structures and microvilli, as well as increasing the expression of TJ proteins. Microarray analysis revealed that MSCs-Exo administration downregulated the level of pro-inflammatory cytokines and upregulated the anti-inflammatory cytokine in colon tissue. MSCs-Exo also modulated the response of Th2 and Th17 cells in the mesenteric lymph nodes (MLN). Reversely, knockdown of TSG-6 abrogated the therapeutic effect of MSCs-Exo on mucosal barrier maintenance and immune regulation, whereas rhTSG-6 administration showed similar efficacy to that of MSCs-Exo.

Conclusions: Our findings suggested that MSCs-Exo protected against IBD through restoring mucosal barrier repair and intestinal immune homeostasis via TSG-6 in mice.
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http://dx.doi.org/10.1186/s13287-021-02404-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164818PMC
May 2021

Transgenic , Containing Individual Genes and Open Reading Frames Under Promoter, Exhibit Compact Habit, Reduced Plant Growth, and Altered Ethylene Tolerance in Flowers.

Front Plant Sci 2021 7;12:672023. Epub 2021 May 7.

Section for Crop Sciences, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Taastrup, Denmark.

Reduced growth habit is a desirable trait for ornamental potted plants and can successfully be obtained through transformation in a stable and heritable manner. Additionally, it can also be obtained by transformation with harboring specific genes from . The bacterial T-DNA harbors four () genes and 14 less known open reading frames (ORFs). The four genes, i.e., A, B, C, and D, are conceived as the common denominator for the compact phenotype and the other less characterized ORFs seem auxiliary but present a potential breeding target for less aberrant and/or more tailored phenotypes. In this study, 'Molly' was transformed with individual genes and selected ORFs in overexpressing cassettes to comprehensively characterize growth traits, gene copy and expression, and ethylene tolerance of the flowers. An association of reduced growth habit, e.g. height and diameter, was observed for B2 and ORF14-2 when a transgene single copy and high gene expression were detected. Chlorophyll content was reduced in overexpressing lines compared to wild type (WT), except for one ΔORF13a (a truncated ORF13a, where SPXX DNA-binding motif is absent). The flower number severely decreased in the overexpressing lines compared to WT. The anthesis timing showed that WT opened the first flower at 68.9 ± 0.9 days and the overexpressing lines showed similar or up to 24 days delay in flowering. In general, a single or low relative gene copy insertion was correlated to higher gene expression, ca. 3 to 5-fold, in B and ΔORF13a lines, while in ORF14 such relation was not directly linked. The increased gene expression observed in B2 and ΔORF13a-2 contributed to reducing plant growth and a more compact habit. Tolerance of detached flowers to 0.5 μl L ethylene was markedly higher for ORF14 with 66% less flower closure at day 3 compared to WT. The subcellular localization of C and ΔORF13a was investigated by transient expression in and confocal images showed that C and ΔORF13a are soluble and localize in the cytoplasm being able to enter the nucleus.
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http://dx.doi.org/10.3389/fpls.2021.672023DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138453PMC
May 2021

Efficacy and safety of umbilical cord mesenchymal stem cells for the treatment of patients with COVID-19.

Clinics (Sao Paulo) 2021 17;76:e2604. Epub 2021 May 17.

Department of Hematology, the Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.

Objectives: The coronavirus disease (COVID-19) outbreak has catastrophically threatened public health worldwide and presented great challenges for clinicians. To date, no specific drugs are available against severe acute respiratory syndrome coronavirus 2. Mesenchymal stem cells (MSCs) appear to be a promising cell therapy owing to their potent modulatory effects on reducing and healing inflammation-induced lung and other tissue injuries. The present pilot study aimed to explore the therapeutic potential and safety of MSCs isolated from healthy cord tissues in the treatment of patients with COVID-19.

Methods: Twelve patients with COVID-19 treated with MSCs plus conventional therapy and 13 treated with conventional therapy alone (control) were included. The efficacy of MSC infusion was evaluated by changes in oxygenation index, clinical chemistry and hematology tests, immunoglobulin (Ig) levels, and pulmonary computerized tomography (CT) imaging. The safety of MSC infusion was evaluated based on the occurrence of allergic reactions and serious adverse events.

Results: The MSC-treated group demonstrated significantly improved oxygenation index. The area of pulmonary inflammation decreased significantly, and the CT number in the inflammatory area tended to be restored. Decreased IgM levels were also observed after MSC therapy. Laboratory biomarker levels at baseline and after therapy showed no significant changes in either the MSC-treated or control group.

Conclusion: Intravenous infusion of MSCs in patients with COVID-19 was effective and well tolerated. Further studies involving a large cohort or randomized controlled trials are warranted.
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http://dx.doi.org/10.6061/clinics/2021/e2604DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8101688PMC
May 2021

The changes in the epidemiology of hand, foot, and mouth disease after the introduction of the EV-A71 vaccine.

Vaccine 2021 06 14;39(25):3319-3323. Epub 2021 May 14.

Nursing College, Guangxi Medical University, Nanning, Guangxi, China. Electronic address:

Three inactive monovalent EV-A71 vaccines have been launched in China since 2016, which may change the HFMD pathogen spectrum and epidemiological trend. Using notifications from the national surveillance system, we analyzed the epidemiological character profiles and the possible pathogen replacement. The proportion of HFMD cases aged 0-12 months decreased from 23.0% to 15.3% between 2013-2015 and 2017-2019 (p < 0.01). EV-A71 among laboratory-confirmed severe cases in 2013-2015 (62.8%) transformed to other EVs (67.2%) in 2017-2019. The age distribution of EV-A71 infection shifted to the older. The cumulative coverage of the EV-A71 vaccine for children aged six months to five years in Guangxi has increased, while in severe cases, the positive rate declined. After gradually expanded vaccination, EV-A71 associated incidence rate, case-severity rate has decreased, and other serotypes are becoming dominant. Thus, bivalent even polyvalent vaccines are urgently needed to control HFMD.
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http://dx.doi.org/10.1016/j.vaccine.2021.05.009DOI Listing
June 2021

Impact of pulmonary rehabilitation in patients with interstitial lung disease in Singapore.

Ann Acad Med Singap 2021 04;50(4):349-352

Department of Respiratory and Critical Care Medicine, Singapore General Hospital, Singapore.

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April 2021

Gas-Forming Psoas Abscess Secondary to Lumbar Spondylodiscitis.

Cureus 2021 Apr 9;13(4):e14388. Epub 2021 Apr 9.

Orthopaedic Surgery, Putrajaya Hospital, Putrajaya, MYS.

Lower back pain is a common presentation in clinical practice. Although most are musculoskeletal in nature, occult spine infection such as spondylodiscitis is commonly missed due to its insidious onset and non-specific symptoms. We report a case of a 63-year-old diabetic woman who presented to our institution's emergency department with altered mental status, nausea, and vomiting. She had a fall one month prior with persistent lower back-pain of increasing intensity. Initial laboratory data revealed an elevated leukocyte count with neutrophil predominance. Plain radiographs showed extensive gas shadows over the chest wall, abdomen, left thigh, and left knee. CT scan revealed L2 compression fracture with spondylodiscitis at L1/L2, left psoas abscess, and extensive subcutaneous emphysema. Open abscess drainage with extensive wound debridement was performed. Intra-operative pus, as well as blood cultures, yielded Escherichia coli. Unfortunately, the patient succumbed to the infection on the seventh day of admission secondary to multi-organ failure.
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http://dx.doi.org/10.7759/cureus.14388DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106948PMC
April 2021

Characterization of the complete mitochondrial genome of the Qiaoke sheep ().

Mitochondrial DNA B Resour 2021 Apr 26;6(4):1514-1515. Epub 2021 Apr 26.

Key Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, China.

Qiaoke sheep () is a local sheep breed in Gansu province, China. It is a kind of Tibetan sheep that used for both meat and wool after long-term breeding. In this paper, the complete mitochondrial genome of Qiaoke sheep was sequenced. The total length of the mitochondrial genome is 16616 bp, and the base composition is 33.65% A, 13.14% G, 25.88% C and 27.33% T. The genome has a total of 37 genes, including 13 protein-coding genes, 22 tRNA genes, two ribosomal RNA genes and a control region (D-loop region). This complete sequence would enlarge useful genomic information for further studies.
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http://dx.doi.org/10.1080/23802359.2021.1914519DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079016PMC
April 2021
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