Publications by authors named "Yi Shen"

1,066 Publications

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Nomograms predict prognosis and hospitalization time using non-contrast CT and CT perfusion in patients with ischemic stroke.

Front Neurosci 2022 22;16:912287. Epub 2022 Jul 22.

Department of Radiology, China-Japan Union Hospital of Jilin University, Changchun, China.

Background: Stroke is a major disease with high morbidity and mortality worldwide. Currently, there is no quantitative method to evaluate the short-term prognosis and length of hospitalization of patients.

Purpose: We aimed to develop nomograms as prognosis predictors based on imaging characteristics from non-contrast computed tomography (NCCT) and CT perfusion (CTP) and clinical characteristics for predicting activity of daily living (ADL) and hospitalization time of patients with ischemic stroke.

Materials And Methods: A total of 476 patients were enrolled in the study and divided into the training set ( = 381) and testing set ( = 95). Each of them owned NCCT and CTP images. We propose to extract imaging features representing as the Alberta stroke program early CT score (ASPECTS) values from NCCT, ischemic lesion volumes from CBF, and TMAX maps from CTP. Based on imaging features and clinical characteristics, we addressed two main issues: (1) predicting prognosis according to the Barthel index (BI)-binary logistic regression analysis was employed for feature selection, and the resulting nomogram was assessed in terms of discrimination capability, calibration, and clinical utility and (2) predicting the hospitalization time of patients-the Cox proportional hazard model was used for this purpose. After feature selection, another specific nomogram was established with calibration curves and time-dependent ROC curves for evaluation.

Results: In the task of predicting binary prognosis outcome, a nomogram was constructed with the area under the curve (AUC) value of 0.883 (95% CI: 0.781-0.985), the accuracy of 0.853, and F1-scores of 0.909 in the testing set. We further tried to predict discharge BI into four classes. Similar performance was achieved as an AUC of 0.890 in the testing set. In the task of predicting hospitalization time, the Cox proportional hazard model was used. The concordance index of the model was 0.700 (SE = 0.019), and AUCs for predicting discharge at a specific week were higher than 0.80, which demonstrated the superior performance of the model.

Conclusion: The novel non-invasive NCCT- and CTP-based nomograms could predict short-term ADL and hospitalization time of patients with ischemic stroke, thus allowing a personalized clinical outcome prediction and showing great potential in improving clinical efficiency.

Summary: Combining NCCT- and CTP-based nomograms could accurately predict short-term outcomes of patients with ischemic stroke, including whose discharge BI and the length of hospital stay.

Key Results: Using a large dataset of 1,310 patients, we show a novel nomogram with a good performance in predicting discharge BI class of patients (AUCs > 0.850). The second nomogram owns an excellent ability to predict the length of hospital stay (AUCs > 0.800).
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http://dx.doi.org/10.3389/fnins.2022.912287DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355636PMC
July 2022

Effect of Body Surface Area on Severe Osteoporotic Fractures: A Study of Osteoporosis in Changsha China.

Front Endocrinol (Lausanne) 2022 22;13:927344. Epub 2022 Jul 22.

Hunan Provincial Key Laboratory of Metabolic Bone Diseases, National Clinical Research Center for Metabolic Diseases, Department of Metabolism and Endocrinology, The Second Xiangya Hospital of Central South University, Changsha, China.

Clinical vertebral fractures and femoral neck fractures are severe osteoporotic fractures that increase morbidity and mortality. Anthropometric variables are associated with an increased risk of osteoporotic fractures, but it is not clear whether body surface area (BSA) has an effect on clinically severe osteoporotic fractures. The study included total of 3,694 cases of clinical vertebral fractures and femoral neck fractures (2,670 females and 1,024 males) and 3,694 controls without fractures who were matched with the cases by sex and age. There was a significant positive correlation between BSA and bone mineral density (BMD) in female and male fracture patients (females: = 0.430-0.471, < 0.001; males: = 0.338-0.414, < 0.001). There was a significant systematic increase in BMD in both genders at various skeletal sites, grouped by BSA quartile. The osteoporosis rates of the lumbar spine (97.9%), femoral neck (92.4%) and total hip (87.1%) in the female Q1 group were significantly higher than those in the Q4 group ( < 0.001), which were 80.0%, 57.9% and 36.9%, respectively, in the Q4 group; the osteoporosis rates of the lumbar spine, femoral neck, and total hip were 53.9%, 59.4%, and 36.3% in the male Q1 group, and 15.2%, 21.9%, and 7.03% in the Q4 group, which were significantly lower than those in the Q1 group ( < 0.001). In age-adjusted Cox regression models, the risk of fracture in the remaining three groups (Q2, Q3, and Q4) for weight, BMI, and BSA for both genders, compared with the highest quartile (Q1 by descending quartile stratification) were significantly higher. In models adjusted for age and BMD, only men in the BSA Q3 (HR = 1.55, 95% CI = 1.09-2.19) and BSA Q4 groups (HR = 1.41, 95% CI = 1.05-1.87) had significantly higher fracture risks. In models adjusted for age, height, weight, BMI, and BSA, low BMD was the greatest fracture risks for both sexes. Our results showed that BSA was closely related to BMD, prevalence of osteoporosis, and fracture risk, and that a decline in BSA may be a new potential risk factor for osteoporotic fractures in Chinese men.
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http://dx.doi.org/10.3389/fendo.2022.927344DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354973PMC
July 2022

Diet-induced inflammation in the anterior paraventricular thalamus induces compulsive sucrose-seeking.

Nat Neurosci 2022 Aug 1;25(8):1009-1013. Epub 2022 Aug 1.

Institute of Neuroscience, NHC and CAMS Key Laboratory of Medical Neurobiology, MOE Frontier Science Center for Brain Research and Brain-Machine Integration, Zhejiang University School of Brain Science and Brain Medicine, Hangzhou, China.

Overconsumption of palatable food may initiate neuroadaptive responses in brain reward circuitry that may contribute to eating disorders. Here we report that high-fat diet (HFD) consumption impedes threat-cue-induced suppression of sucrose-seeking in mice. This compulsive sucrose-seeking was due to enhanced cue-triggered neuronal activity in the anterior paraventricular thalamus (aPVT) resulting from HFD-induced microglia activation. Thus, metabolic inflammation in the aPVT produces an adaptive response to threat cues, leading to compulsive food-seeking.
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http://dx.doi.org/10.1038/s41593-022-01129-yDOI Listing
August 2022

Roles of Oxygen Species in Low-Temperature Catalytic -Xylene Oxidation on MOF-Derived Bouquetlike CeO.

ACS Appl Mater Interfaces 2022 Jul 29. Epub 2022 Jul 29.

Key Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province, College of Environment, Zhejiang University of Technology, Hangzhou 310014, China.

To realize efficient low-temperature catalytic -xylene oxidation, MOF-derived CeO-X catalysts were prepared via the pyrolysis of MOF precursors with different ratios of cerium nitrate to trimesic acid. Among the synthesized catalysts, the bouquet like CeO-1 exhibited the best activity with and of 156 and 198 °C and the lowest activation energy of 60.67 kJ·mol (WHSV= 48 000 mL·g·h, -xylene concentration = 500 ppm). -Xylene was completely mineralized, and no change in conversion efficiency or CO yield was found at 5 vol % HO for over 50 h. The rich active oxygen species (XPS: O/O = 0.69) and abundant oxygen vacancies (Raman: / = 0.036) of CeO-1 made crucial contribution to its superior catalytic activity. The O-TPD and H-TPR results confirmed that CeO-1 had more surface active oxygen and better mobility of bulk oxygen. Moreover, the reaction routes under different atmospheres were probed through DRIFTS, in which oxygen vacancy played a key role in promoting the adsorption and activation of molecular oxygen and facilitating the migration of the bulk lattice oxygen.
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http://dx.doi.org/10.1021/acsami.2c08418DOI Listing
July 2022

FIGNL1 Inhibits Non-homologous Chromosome Association and Crossover Formation.

Front Plant Sci 2022 11;13:945893. Epub 2022 Jul 11.

Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, China.

Meiotic crossovers (COs) not only generate genetic diversity but also ensure the accuracy of homologous chromosome segregation. Here, we identified FIGNL1 as a new inhibitor for extra crossover formation in rice. The mutant displays abnormal interactions between non-homologous chromosomes at diakinesis, and chromosome bridges and fragmentation at subsequent stages of meiosis, but shows normal homologous chromosome pairing and synapsis during early prophase I. FIGNL1 participates in homologous chromosome recombination and functions downstream of DMC1. Mutation of increases the number of bivalents in mutants, but does not change the number of HEI10 foci, indicating that FIGNL1 functions in limiting class II CO formation. FIGNL1 interacts with MEICA1, and colocalizes with MEICA1 in a dynamic pattern as punctate foci located between two linear homologous chromosomes. The localization of FIGNL1 depends on ZEP1-mediated assembly of the synaptonemal complex. Based on these results, we propose that FIGNL1 inhibits non-homologous chromosome interaction and CO formation during rice meiosis.
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http://dx.doi.org/10.3389/fpls.2022.945893DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9310568PMC
July 2022

Comparative Characterization of Different Molecular Formats of Bispecific Antibodies Targeting EGFR and PD-L1.

Pharmaceutics 2022 Jun 29;14(7). Epub 2022 Jun 29.

Division of Biotechnology Review and Research 1, Office of Biotechnology Products, Office of Pharmaceutical Quality, Center for Drug Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, MD 20993, USA.

We generated two IgG1-like bispecific antibodies (BsAbs) with different molecular formats, symmetrical DVD-Ig and asymmetrical knob-in-hole (KIH), targeting the same antigens, EGFR and PD-L1 (designated as anti-EGFR/PD-L1). We performed the physiochemical and biological characterization of these two formats of anti-EGFR/PD-L1 BsAbs and compared some key quality attributes and biological activities of these two formats of BsAbs. Physiochemical binding characterization data demonstrated that both formats bound EGFR and PD-L1. However, the binding affinity of the KIH format was weaker than the DVD-Ig format in Biacore binding assays. In contrast, both DVD-Ig and KIH BsAbs had similar ELISA and cell surface binding activities, comparable to mAbs. Triple-negative breast cancer (TNBC) cells and a xenograft model were used to test the potency of BsAbs and other biological activities. Results showed that anti-EGFR/PD-L1 BsAbs exhibited in vitro and in vivo antitumor proliferation activity, but there was a difference in the potencies of the respective BsAb formats (DVD-Ig and KIH) when different cells or assays were used. This study provides evidence that the potency of the BsAbs targeting the same antigens can be affected by the respective molecular features, and selection of appropriate cell lines and assays is critically important for the assay development and potency testing of BsAbs.
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http://dx.doi.org/10.3390/pharmaceutics14071381DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9325241PMC
June 2022

First report on the burden and distribution of Cu, Zn, Pb, and Cd in the Ocellated icefish (Chionodraco rastrospinosus) of northern Antarctic Peninsula.

Authors:
Yi Shen Guoping Zhu

Mar Pollut Bull 2022 Jul 22;182:113963. Epub 2022 Jul 22.

College of Marine Sciences, Shanghai Ocean University, Shanghai 201306, China; Center for Polar Research, Shanghai Ocean University, Shanghai 201306, China; Polar Marine Ecosystem Group, The Key Laboratory of Sustainable Exploitation of Oceanic Fisheries Resources, Ministry of Education, Shanghai 201306, China; National Engineering Research Center for Oceanic Fisheries, Shanghai 201306, China. Electronic address:

Understanding the distribution of trace elements in Ocellated icefish (Chionodraco rastrospinosus), one of fish species with lacking hemoglobin from the family Channichthyidae and distributes in a very limited area at the south Scotia Sea, will help understand their physiological composition and conserve this vulnerable population; however, information on this topic is extremely limited. This study examines trace elements (two essential elements, copper [Cu] and zinc [Zn], and two non-essential elements, cadmium [Cd] and lead [Pb]) in C. rastrospinosus and provides for the first time baseline data on elemental distribution in four tissues of C. rastrospinosus in the northern Antarctic Peninsula (NAP). The element concentrations showed the following trends: Zn > Cu > Pb > Cd in muscle and stomach and Zn > Cu > Cd > Pb in intestine and liver. Among all tissues, muscle had the lowest element concentrations. The average Zn level is 70.81 ± 28.91 μg g dry weight (DW) in C. rastrospinosus muscle, which is higher significantly than average levels of Cu (0.56 ± 0.41 μg g DW), Pb (0.29 ± 0.33 μg g DW) and Cd (0.12 ± 0.05 μg g DW). Zn and Cd concentrations in the stomach and intestines were significantly positively correlated. C. rastrospinosus could be a useful bioindicator for monitoring variability in trace elements dynamics in NAP and the environmental variability in this region.
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http://dx.doi.org/10.1016/j.marpolbul.2022.113963DOI Listing
July 2022

Overexpression of 14-3-3ζ primes disease recurrence, metastasis and resistance to chemotherapy by inducing epithelial-mesenchymal transition in NSCLC.

Aging (Albany NY) 2022 Jul 22;14(14):5838-5854. Epub 2022 Jul 22.

Department of Cardiothoracic Surgery, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China.

The prognosis of non-small cell lung cancer (NSCLC) is disappointing because disease recurrence and distant metastasis inevitably occurred. The aim of the present study is to identify novel biomarkers predicting tumor recurrence and metastasis. The 14-3-3ζ protein has been extensively described as a tumor promoter in a panel of solid tumors, including NSCLC. However, there is a big gap regarding the knowledge between 14-3-3ζ and NSCLC recurrence. In this study, we found that overexpression of 14-3-3ζ was more frequent in NSCLC tumor tissues with tumor recurrence. By using scratch healing assay and transwell assay, we demonstrated that NSCLC cells with high expression of 14-3-3ζ gained increased motile and invasive capacity, whereas siRNA-mediated knockdown of endogenous 14-3-3ζ abrogated cancer cell dissemination. Intriguingly, we found that NSCLC cells underwent epithelial-mesenchymal transition (EMT) after the induction of 14-3-3ζ and . These findings could be readily recaptured in clinical setting since we showed that NSCLC tumor specimen with high expression of 14-3-3ζ revealed biological features of EMT. Overexpression of 14-3-3ζ also enhanced the phosphorylation of Akt and promoted the proliferation of NSCLC cell lines. In agreement with this notion, we reported that NSCLC cells with high expression of 14-3-3ζ became resistant to chemotherapy-induced apoptosis. These findings strongly suggested that 14-3-3ζ as a novel biomarker predicting risks of disease recurrence and screening 14-3-3ζ status may be a promising approach to select patients who experienced high risks of cancer recurrence and resistance to chemotherapy.
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http://dx.doi.org/10.18632/aging.204188DOI Listing
July 2022

Design of International Chinese Education Promotion Platform Based on Artificial Intelligence and Facial Recognition Technology.

Authors:
Yi Shen Sixian Sun

Comput Intell Neurosci 2022 13;2022:6424984. Epub 2022 Jul 13.

Chiang Rai Rajabhat University, Chiang Rai 57100, Thailand.

With the continuous development of today's society, digital image processing technology has been applied in more and more fields, among which authentication in digital image processing technology has become a hot field. In the process of identity verification, the face is used as the basis of feature recognition because the method of using the face as a feature basis is more easily accepted by the public and the operation is simple and the feasibility is stronger. In the online education model, observing and comparing students' facial emotions through the platform and analyzing students' learning goals, learning effects, learning emotions, and contradictions and conflicts arising in the process of cooperation have become an effective teaching evaluation system. Up to now, China has developed into the second largest economy in the world. The global "Chinese fever" has brought China's culture into a new stage of development. Countries in the world learn Chinese culture by developing Chinese language courses. By building a Chinese learning intelligent system with a B/S structure, this system can effectively evaluate the teaching process. It can be seen from the test results that the platform meets the basic requirements of functional design.
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http://dx.doi.org/10.1155/2022/6424984DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9300334PMC
July 2022

Blood Cells and Venous Thromboembolism Risk: A Two-Sample Mendelian Randomization Study.

Front Cardiovasc Med 2022 8;9:919640. Epub 2022 Jul 8.

State Key Laboratory of Respiratory Disease, National Center for Respiratory Medicine, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.

Background: Previous studies have shown that various cell indices are associated with a higher risk of venous thromboembolism (VTE), however, whether these findings reflect a causal relationship remains unclear. Therefore, we performed a two-sample Mendelian randomization (MR) analysis to assess the causal association of various blood cells with VTE risk.

Study Design And Methods: Summary statistics of genetic instruments representing cell indices for erythrocytes, leukocytes, and platelets were extracted from genome-wide association studies of European ancestry, by Two-Sample Mendelian Randomization. Inverse variance weighting (IVW) was used as the primary analytical method for MR. Sensitivity analyses were performed to detect horizontal pleiotropy and heterogeneity.

Results: Genetically predicted red blood cell distribution width, mean reticulocyte volume, and mean red blood cell volume were positively associated with VTE, with odds ratio (OR) of 1.002 [CI 1.000-1.003, = 0.022), 1.003 (CI 1.001-1.004, = 0.001, respectively)] and 1.001 (CI 1.000-1.002, = 0.005). Genetically predicted monocyte count was negatively correlated with VTE, with OR = 0.998 (CI 0.996-0.999, = 0.041).

Conclusion: Genetically liability to high- red blood cell distribution width, mean reticulocyte volume, mean red blood cell volume, and low monocyte count are associated with the higher risk of VTE. Targeting these factors might be a potential strategy to prevent VTE.
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http://dx.doi.org/10.3389/fcvm.2022.919640DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304581PMC
July 2022

Bacteria-induced amino acid metabolisms involve in appearance characteristics of high-temperature Daqu.

J Sci Food Agric 2022 Jul 20. Epub 2022 Jul 20.

Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China.

Background: There were significant changes occurred in Daqu bricks on the 15th day of incubation and the colors of bricks (yellow, brown, and dark) were generally used as one of the standards for the quality evaluation by experienced workers. This study aimed to explore basis behind the phenomenon by multi-omics studies. The physicochemical properties of different high-temperature Daqu were compared. Furthermore, PacBio sequencing and UHPLC-QE-MS approach was employed to analyze the differences in the microbiome and metabolome among different Daqu.

Results: Bacillus was the biomarker of yellow Daqu, Thermoactinomyces and Thermoascus were the key genera in brown Daqu, and Burkholderiales, Sphingomonas, and Ralstonia were biomarkers in dark Daqu. The physicochemical characteristics (especially the color values) of different high-temperature Daqu, showed strong correlations with the bacterial alpha diversity and the relative abundance of bacterial dominant genera. Amino acid metabolism pathways including tryptophan metabolism, beta-alanine metabolism, and arginine biosynthesis were the key factors resulting in the characteristic differences where Bacillus, Burkholderia, Ralstonia, and Sphingomonas were pivotal bacterial genera. The relative abundance of Bacillus had a positive correlation with the content of 3-hydroxykynurenamine, L-glutamic acid, and pantothenic acid while it showed a negative correlation with indoleacetic acid, L-tryptophan, N-acetylserotonin, L-histidine, L-aspartic acid, phosphatidylserine, 5-methoxyindoleacetate, and L-serine. Burkholderia, Ralstonia, and Sphingomonas had the opposite effects.

Conclusion: Microbes played different roles in amino acid metabolism pathways, resulting in the production of different metabolites, which contributed to the differences in Daqu appearance and quality. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/jsfa.12136DOI Listing
July 2022

Direct Determination of Free Nicotine Content in Tobacco.

ACS Omega 2022 Jul 23;7(27):23061-23068. Epub 2022 Jun 23.

Technology Center, China Tobacco Sichuan Industrial Co., Ltd., No. 56, Section 1 of Cheng Long Avenue, Jinjiang District, Chengdu 610066, China.

The accurate determination of the free nicotine content in tobacco is of great importance for the quality assessment of tobacco leaves and cigarettes, as well as for the study of tar reduction and cigarette formulation. A method based on solvent extraction and gas chromatography-mass spectrometry (GC-MS) was established for direct determination of free nicotine content in tobacco. Free nicotine extraction from tobacco was optimized, using different solvents and physical techniques (shaking, ultrasonic oscillation, and static extraction). Ultimately, a 24 h static extraction with cyclohexane, followed by GC-MS analysis, gave superior results. The standard addition recovery was 98.0-104.7%, with a limit of detection of 5.3 μg/g and a relative standard deviation between 1.3 and 4.1% ( = 5). Quadratic regression of the standard curve was excellent ( ≥ 0.9994). The free nicotine content was determined in 67 tobacco samples, with parallel samples showing relative deviations of 0.1-3.1%. To evaluate the effect of nicotine salts present in tobacco, malic acid, citric acid, acetic acid, and nicotine citrate were spiked into samples prior to extraction and analysis. The results show no interference from bound nicotine compounds on the method. The experimental results show that direct determination of free nicotine in tobacco was achieved using a simple static cyclohexane extraction. Moreover, the high extraction efficiency of free nicotine was also achieved with ease of operation and good repeatability.
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http://dx.doi.org/10.1021/acsomega.2c00089DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9280935PMC
July 2022

Pyroptosis: A Novel Intervention Target in the Progression of Osteoarthritis.

J Inflamm Res 2022 11;15:3859-3871. Epub 2022 Jul 11.

Department of Orthopaedics, The Second Xiangya Hospital of Central South University, Changsha, People's Republic of China.

Osteoarthritis (OA) is one of the most common chronic joint diseases and is gradually becoming the main cause of disability and joint pain in the elderly worldwide. Pyroptosis is a regulated programmed cell death triggered by inflammasomes. It leads to cell swelling, lysis, and bioactive molecule secretion. Studies found that the damaged chondrocytes in OA joints had morphological characteristics of pyroptosis, and the cytokines associated with pyroptosis in synovial fluid increased, indicating that pyroptosis may have certain impacts on the pathological progression of OA. This review briefly summarizes the molecular mechanisms of pyroptosis and the epidemiology and pathogenesis of OA. Furthermore, we discussed the role of pyroptosis in articular cartilage and synovium during OA and reviewed the progress of pyroptosis-related molecules in the targeted therapy of OA joints, hoping to provide feasible directions for the diversified treatment of OA.
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http://dx.doi.org/10.2147/JIR.S368501DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9285853PMC
July 2022

Effects of ultrasound-guided erector spinae plane block with dexmedetomidine combined with ropivacaine of the same dose and different concentrations on analgesic effect and rehabilitation quality of patients undergoing thoracoscopic wedge resection of the lung: a prospective, randomized, controlled trial.

BMC Anesthesiol 2022 07 16;22(1):225. Epub 2022 Jul 16.

Department of Anesthesiology, Longyan First Affiliated Hospital of Fujian Medical University, Jiuyi North Road, Xinluo District, Longyan, 364000, Fujian, China.

Objective: To investigate the analgesic effect and rehabilitation quality of patients undergoing thoracoscopic wedge resection of the lung under erector spinae plane (ESP) block with dexmedetomidine combined with the same dose and different concentrations of ropivacaine.

Methods: Seventy patients undergoing thoracoscopic wedge resection were randomly divided into groups A (n = 35) and B (n = 35). To perform ESP block, the groups were administered dexmedetomidine (0.5 μg/kg) combined with 30 mL of 0.33% ropivacaine or 20 mL of 0.5% ropivacaine, respectively, half an hour before general anesthesia induction. We compared the onset time of anesthesia, the block level, and the duration of the block between the two groups. The number of compressions of the analgesic pump within 24 h and 48 h postoperatively and the time of the first compression were noted. The visual analog scale (VAS) scores of static and cough at 0.5 h, 6 h, 12 h, 24 h, and 48 h postoperatively were noted. Furthermore, the 40-item quality of recovery questionnaire (QoR-40) score was recorded at 24 h postoperatively. In addition, we noted the time taken to get out of the bed for the first time, the length of hospital stay, analgesia satisfaction, and the occurrence of related adverse reactions and complications within 48 h postoperatively.

Results: The range of ESP block was wider in Group A than in Group B (P < 0.05). Group B had a significantly shorter onset time (P < 0.05) and lower static and cough VAS scores at 6 h and 12 h postoperatively (P < 0.05); this was associated with significantly fewer compressions of the analgesic pump within 24 h and 48 h postoperatively and significantly more time until the first compression of the analgesic pump was required (P < 0.05). Group B was associated with significantly superior QoR-40 scores 24 h postoperatively and significantly shorter time to get out of the bed for the first time than Group A (P < 0.05).

Conclusion: Dexmedetomidine combined with 0.5% ropivacaine for ESP block is better than 0.33% ropivacaine for overall analgesia and postoperative rehabilitation of patients undergoing thoracoscopic wedge resection.

Trial Registration: ChiCTR2200058114 , Date of registration: 30/03/2022.
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http://dx.doi.org/10.1186/s12871-022-01768-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9288034PMC
July 2022

Ritter-type amination of C(sp)-H bonds enabled by electrochemistry with SO.

Nat Commun 2022 Jul 16;13(1):4138. Epub 2022 Jul 16.

Engineering Research Centre of Pharmaceutical Process Chemistry, Ministry of Education; Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China.

By merging electricity with sulfate, the Ritter-type amination of C(sp)-H bonds is developed in an undivided cell under room temperature. This method features broad substrate generality (71 examples, up to 93% yields), high functional-group compatibility, facile scalability, excellent site-selectivity and mild conditions. Common alkanes and electron-deficient alkylbenzenes are viable substrates. It also provides a straightforward protocol for incorporating C-deuterated acetylamino group into C(sp)-H sites. Application in the synthesis or modification of pharmaceuticals or their derivatives and gram-scale synthesis demonstrate the practicability of this method. Mechanistic experiments show that sulfate radical anion, formed by electrolysis of sulfate, served as hydrogen atom transfer agent to provide alkyl radical intermediate. This method paves a convenient and flexible pathway for realizing various synthetically useful transformations of C(sp)-H bonds mediated by sulfate radical anion generated via electrochemistry.
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http://dx.doi.org/10.1038/s41467-022-31813-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9288499PMC
July 2022

Blocking glycine utilization inhibits multiple myeloma progression by disrupting glutathione balance.

Nat Commun 2022 Jul 11;13(1):4007. Epub 2022 Jul 11.

Haihe Laboratory of Cell Ecosystem, State Key Laboratory of Experimental Hematology, Key Laboratory for Carcinogenesis and Invasion, Chinese Ministry of Education, Key Laboratory of Carcinogenesis, Chinese Ministry of Health, Department of Hematology, Xiangya Hospital, Central South University, Changsha, Hunan, China.

Metabolites in the tumor microenvironment are a critical factor for tumor progression. However, the lack of knowledge about the metabolic profile in the bone marrow (BM) microenvironment of multiple myeloma (MM) limits our understanding of MM progression. Here, we show that the glycine concentration in the BM microenvironment is elevated due to bone collagen degradation mediated by MM cell-secreted matrix metallopeptidase 13 (MMP13), while the elevated glycine level is linked to MM progression. MM cells utilize the channel protein solute carrier family 6 member 9 (SLC6A9) to absorb extrinsic glycine subsequently involved in the synthesis of glutathione (GSH) and purines. Inhibiting glycine utilization via SLC6A9 knockdown or the treatment with betaine suppresses MM cell proliferation and enhances the effects of bortezomib on MM cells. Together, we identify glycine as a key metabolic regulator of MM, unveil molecular mechanisms governing MM progression, and provide a promising therapeutic strategy for MM treatment.
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http://dx.doi.org/10.1038/s41467-022-31248-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273595PMC
July 2022

Calycosin Improves Intestinal Mucosal Barrier Function after Gastrectomy in Rats through Alleviating Bacterial Translocation, Inflammation, and Oxidative Stress.

Evid Based Complement Alternat Med 2022 26;2022:7412331. Epub 2022 Jun 26.

Department of General Surgery, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei 230031, Anhui, China.

Objective: Calycosin is the main bioactive extract of with anti-inflammation, antioxidant, and anticancer properties. Here, our study evaluated the protective effects and mechanisms of calycosin on intestinal mucosal barrier under gastrectomy.

Methods: After receiving gastrectomy, the rats were administrated with 20 mg/kg, 40 mg/kg, or 80 mg/kg calycosin. Endotoxin, bacterial translocation, and intestinal bacterial flora were assayed. Intestinal injury was detected via hematoxylin and eosin staining. Tight junction indicators (occludin, claudin, and ZO-1) and apoptotic proteins (Bax, Bcl-2, and cleaved caspase 3) were examined in intestinal tissues. Inflammatory indicators (IL-1, IL-6, and TNF-) were examined in serum or intestinal specimens via ELISA. Apoptosis was assessed via TUNEL staining. IgA + B cells in intestinal tissues and sIgA in intestinal lumen were examined through immunohistochemistry and ELISA, respectively. Oxidative stress indicators (TSH, SOD, CAT, GSH-Px, and MDA) were also detected via ELISA.

Results: Our results showed that calycosin administration decreased endotoxin levels in peripheral blood, intestine, and portal vein blood; lowered the bacterial translocation ratio; and regained the balance among intestinal bacterial flora (comprising bifidobacterium, lactic acid bacillus, enterobacter, enterococcus, aerobic bacteria, and anaerobic bacteria) in the rats with gastrectomy. After calycosin treatment, intestinal mucosal damage of the rats with gastrectomy was ameliorated, with the increase in expression of tight junction proteins. Additionally, calycosin reduced intestinal inflammation, apoptosis, secretion of sIgA, and oxidative stress in the rats with gastrectomy.

Conclusion: Altogether, our findings demonstrate that calycosin may improve intestinal mucosal barrier function under gastrectomy via reducing bacterial translocation, inflammation, and oxidative stress.
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http://dx.doi.org/10.1155/2022/7412331DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9251107PMC
June 2022

Recent Advances in Microgels: From Biomolecules to Functionality.

Small 2022 Jul 5:e2200180. Epub 2022 Jul 5.

Centre for Misfolding Diseases, Yusuf Hamied Department of Chemistry, University of Cambridge, Cambridge, CB2 1EW, UK.

The emerging applications of hydrogel materials at different length scales, in areas ranging from sustainability to health, have driven the progress in the design and manufacturing of microgels. Microgels can provide miniaturized, monodisperse, and regulatable compartments, which can be spatially separated or interconnected. These microscopic materials provide novel opportunities for generating biomimetic cell culture environments and are thus key to the advances of modern biomedical research. The evolution of the physical and chemical properties has, furthermore, highlighted the potentials of microgels in the context of materials science and bioengineering. This review describes the recent research progress in the fabrication, characterization, and applications of microgels generated from biomolecular building blocks. A key enabling technology allowing the tailoring of the properties of microgels is their synthesis through microfluidic technologies, and this paper highlights recent advances in these areas and their impact on expanding the physicochemical parameter space accessible using microgels. This review finally discusses the emerging roles that microgels play in liquid-liquid phase separation, micromechanics, biosensors, and regenerative medicine.
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http://dx.doi.org/10.1002/smll.202200180DOI Listing
July 2022

Profunda Artery Perforator Flaps From the Posteromedial Region of the Thigh for Head and Neck Reconstruction.

Otolaryngol Head Neck Surg 2022 Jul 5:1945998221109145. Epub 2022 Jul 5.

Department of CT Clinical Research, CT Business Unit, Canon Medical Systems Co, Ltd, Beijing, China.

Objective: Profunda femoris artery perforator flaps (PAPFs) have not been widely used in head and neck reconstructions. The feasibility and outcomes of PAPFs for various head and neck reconstructions need to be investigated.

Study Design: Retrospective analysis.

Setting: A single-institution review.

Methods: PAPFs were utilized in head and neck reconstructions from 2019 to 2021. Local anatomy, surgical technique, and complications were discussed. Chimeric PAPF applications with muscle components were described for coverage of extensive multiunit defects. Additionally, aesthetic and functional outcomes were compared with anterolateral thigh perforator flaps.

Results: A total of 33 cases were included. The average age was 54.2 years (range, 30-74). The most common underlying pathology was oral squamous cell carcinoma (n = 26, 78.8%), while the mean ± SD body mass index was 25.4 ± 2.8 kg/m. Middle perforators (n = 14, 42.4%) were the most commonly utilized ones. The perforator-based chimeric/composite applications were used in 9 (27.3%), with the muscular components consisting of gracilis (n = 3, 9.1%), adductor magnus (n = 5, 15.2%), or semimembranosus muscles (n = 1, 3.0%). Venous thromboses of the PAPFs were found in 2 (6.1%), though salvaged. The occurrence of postoperative 90-day morbidity (complication) was related to mandibulectomy/maxillectomy ( = .020). Postoperative validated questionnaires showed a trend of intermediate to high scores, indicating noninferior outcomes in several categories, when compared with the anterolateral thigh perforator flap counterparts.

Conclusion: PAPFs are a good reconstructive alternative for intermediate to large head and neck reconstructions. Besides, PAPFs can provide sufficient tissue volume and versatility of potentially incorporating adjacent muscle components.
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http://dx.doi.org/10.1177/01945998221109145DOI Listing
July 2022

Reduced astrocytic mGluR5 in the hippocampus is associated with stress-induced depressive-like behaviors in mice.

Neurosci Lett 2022 07 30;784:136766. Epub 2022 Jun 30.

Department of Neurobiology and Department of General Intensive Care Unit of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310058, China; NHC and CAMS Key Laboratory of Medical Neurobiology, MOE Frontier Science Center for Brain Research and Brain-Machine Integration, School of Brain Science and Brain Medicine, Zhejiang University, Hangzhou 310058, China; National Health and Disease Human Brain Tissue Resource Center, Hangzhou 310058, China. Electronic address:

Major depressive disorder (MDD) is one of the most common and disabling mental disorders that characterized by profound disturbances in emotional regulation, motivation, cognition, and the physiology of affected individuals. Although MDD was initially thought to be primarily triggered through neuronal dysfunction, the pathological alterations in astrocytic function have been previously reported in MDD. We report that chronic restraint stress (CRS) induces astrocyte activation and decreases expression of astrocytic mGluR5 in the hippocampal CA1 of susceptible mice exhibited depressive-like behaviors. Reducing expression of astrocytic mGluR5 in dorsal CA1 simulates CRS-induced depressive-like behaviors and impairs excitatory synaptic function in mice, while overexpression of astrocytic mGluR5 in dorsal CA1 rescues CRS-induced depressive-like traits and excitatory synaptic dysfunction. Thus, we provide direct evidence for an important role of astrocytic mGluR5 in producing the behavioral phenotypes of MDD, supporting astrocytic mGluR5 may serve as an effective therapeutic target for MDD.
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http://dx.doi.org/10.1016/j.neulet.2022.136766DOI Listing
July 2022

Comparative analysis of malnutrition diagnosis methods in lung cancer patients using a Bayesian latent class model.

Asia Pac J Clin Nutr 2022 ;31(2):181-190

Department of Epidemiology and Health Statistics, School of Public Health, Beijing Municipal Key Laboratory of Clinical Epidemiology, Capital Medical University, Beijing, China. Email:

Background And Objectives: There are no consensus criteria for malnutrition diagnosis in clinical settings, the Global Leadership Initiative on Malnutrition (GLIM) criteria were developed to facilitate global comparisons of malnutrition prevalence, interventions and outcomes. Validation to assess usefulness in clinical practice is essential, however, the imperfect nature of reference standards used in concurrent validation may result in biased estimates of diagnostic accuracy. The Bayesian latent class model (BLCM) can assess the diagnostic performance when a "gold standard" is absent. This study's objective was to assess the diagnostic performance of the GLIM criteria in comparison with the Nutritional Risk Screening 2002 (NRS-2002) and the Patient Generated Subjective Global Assessment (PG-SGA) in lung cancer patients using a BLCM. We hypothesized that the GLIM criteria are more sensitive and specific for malnutrition diagnosis in lung cancer patients.

Methods And Study Design: 1,384 patient records retrospectively obtained from the "Investigation on Nutrition Status and its clinical outcome of common Cancers" (INSCOC) study were used to determine the prevalence of malnutrition, sensitivity (Se) and specificity (Sp) by applying a BLCM.

Results: The prevalence of malnutrition was 0.56. The sensitivity and specificity of the GLIM criteria were Se: 0.85 and Sp: 0.88; Se: 0.74 and Sp: 0.85 for NRS-2002 and Se: 0.96 and Sp: 0.89 for PG-SGA.

Conclusions: Although the GLIM criteria were acceptable for malnutrition diagnosis, PG-SGA is superior for determining cancer-associated malnutrition. Because of its fair sensitivity, NRS-2002 was best equipped to screen out patients not at nutritional risk.
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http://dx.doi.org/10.6133/apjcn.202206_31(2).0003DOI Listing
July 2022

A dynamic nomogram for predicting survival among diabetic patients on maintenance hemodialysis.

Ther Apher Dial 2022 Jun 22. Epub 2022 Jun 22.

Department of Nephrology, Affiliated Hospital of Nantong University, Nantong, Jiangsu, P.R. China.

Introduction: Among maintenance hemodialysis (MHD) patients, ones with diabetes mellitus (DM) are known to have the worst outcome.

Methods: A total of 263 MHD patients were included, a dynamic nomogram was established based on multivariable Cox regression analysis.

Results: The median overall survival (OS) time was 46 months. The 1-, 3-, and 5-year OS rates were 90.9%, 70.5% and 53.9%, respectively. The multivariable Cox regression analysis indicated that DM duration, cardiovascular complication, baseline values before starting MHD for estimated glomerular filtration rate and serum phosphate were independent risk factors. The C-index of the dynamic nomogram was 0.745 and the calibration curves showed optimal agreement between the model prediction and actual observation for predicting survival probabilities.

Conclusions: Our study was the first to establish dynamic nomogram among diabetic MHD patients, the fast and convenient online tool can be used for individual risk estimation at the point of prognosis prediction.
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http://dx.doi.org/10.1111/1744-9987.13901DOI Listing
June 2022

Aging can transform single-component protein condensates into multiphase architectures.

Proc Natl Acad Sci U S A 2022 06 21;119(26):e2119800119. Epub 2022 Jun 21.

Maxwell Centre, Cavendish Laboratory, Department of Physics, University of Cambridge, Cambridge CB3 0HE, United Kingdom.

Phase-separated biomolecular condensates that contain multiple coexisting phases are widespread in vitro and in cells. Multiphase condensates emerge readily within multicomponent mixtures of biomolecules (e.g., proteins and nucleic acids) when the different components present sufficient physicochemical diversity (e.g., in intermolecular forces, structure, and chemical composition) to sustain separate coexisting phases. Because such diversity is highly coupled to the solution conditions (e.g., temperature, pH, salt, composition), it can manifest itself immediately from the nucleation and growth stages of condensate formation, develop spontaneously due to external stimuli or emerge progressively as the condensates age. Here, we investigate thermodynamic factors that can explain the progressive intrinsic transformation of single-component condensates into multiphase architectures during the nonequilibrium process of aging. We develop a multiscale model that integrates atomistic simulations of proteins, sequence-dependent coarse-grained simulations of condensates, and a minimal model of dynamically aging condensates with nonconservative intermolecular forces. Our nonequilibrium simulations of condensate aging predict that single-component condensates that are initially homogeneous and liquid like can transform into gel-core/liquid-shell or liquid-core/gel-shell multiphase condensates as they age due to gradual and irreversible enhancement of interprotein interactions. The type of multiphase architecture is determined by the aging mechanism, the molecular organization of the gel and liquid phases, and the chemical makeup of the protein. Notably, we predict that interprotein disorder to order transitions within the prion-like domains of intracellular proteins can lead to the required nonconservative enhancement of intermolecular interactions. Our study, therefore, predicts a potential mechanism by which the nonequilibrium process of aging results in single-component multiphase condensates.
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http://dx.doi.org/10.1073/pnas.2119800119DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9245653PMC
June 2022

Robust Transmit Beamforming Algorithm for Low-Altitude Slow-Speed Small Target Detection.

Comput Intell Neurosci 2022 8;2022:6358133. Epub 2022 Jun 8.

School of Information and Science Technology, Shijiazhuang Tiedao University, Shijiazhuang 050043, China.

Strong ground clutter echoes make it difficult to detect low-altitude slow-speed small (LSS) targets. To suppress ground clutter effectively in LSS target detection, a robust transmit beamforming algorithm has been proposed in this paper. Sidelobes in the ground side can be cut down, with the excess energy concentrated on the air side, which would be cleaner and simpler. The objective function is a second-order cone programming problem and can be solved by the convex optimization algorithm. With the consideration of taking full advantage of transmit power, the weight vector is further processed under the unimodular constraint. Numerical experiments are carried out to demonstrate the validity and superiority of the proposed method.
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http://dx.doi.org/10.1155/2022/6358133DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200524PMC
June 2022

Effect of Bairui Granule on Inflammatory Mediators in Induced Sputum, Leukotriene C4, and EOS in Peripheral Blood of Children with Cough Variant Asthma.

Comput Math Methods Med 2022 8;2022:2657994. Epub 2022 Jun 8.

Department of Paediatrics, The Affiliated Suzhou Science &Technology Town Hospital of Nanjing Medical University, China.

Objective: To study the effect of Hanchuan Zupa granule combined with conventional western medicine in the treatment of children with bronchial asthma.

Methods: 98 cases in Fengrun District People's Hospital of Tangshan City from June 2018 to February 2021 were selected. The control group was given oxygen therapy, antibiotics, and aerosol inhalation of quick acting 2 receptor agonist, glucocorticoid, and other conventional western medicine treatment, while the observation group was treated with Bairui granule on the basis of the control group. The course of treatment of the two groups was 1 week.

Results: After treatment, the levels of sputum IL-4, IL-17, neu, and ECP in the two groups decreased, and the observation group was lower than the control group ( < 0.05). The levels of EOS, CXCR4, LTB4, and SDF-1 in peripheral blood of the two groups were lower than those in the control group ( < 0.05). The daytime cough, night cough, and TCM syndrome scores of the two groups were decreased, and the observation group was lower than the control group ( < 0.05).

Conclusion: Bairui granule combined with conventional western medicine in the treatment of children with bronchial asthma, the curative effect is worthy of affirmation, can effectively improve cough symptoms, reduce EOS, CXCR, LTB4, SDF-1 levels, inhibit airway inflammation, and has good clinical application value.
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http://dx.doi.org/10.1155/2022/2657994DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200585PMC
June 2022

Research on Portal Venous Hemodynamics and Influencing Factors of Portal Vein System Thrombosis for Wilson's Disease after Splenectomy.

Front Surg 2022 30;9:834466. Epub 2022 May 30.

The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, China.

Objective: Splenectomy is one crucial solution for hypersplenism with portal hypertension. However, portal vein system thrombosis (PVST) caused by hemodynamic changes affects the prognosis of patients. We analyze the changes in portal vein hemodynamics following splenectomy for Wilson's disease combined with portal hypertension and the influencing factors that lead to PVST.

Methods: A retrospective cohort study was conducted, in which 237 Wilson's disease patients with hypersplenism underwent splenectomy. The hemodynamic indices of the portal vein were monitored before surgery and on the 1st, 7th, and 14th days around surgery. The patients were divided into PVST and non-PVST groups. The clinical factors were identified by univariate and multivariate logistic regression. The Logit was calculated according to the logistic regression prediction model, and the ROC curve for each independent factor was plotted.

Results: The portal vein velocity, flow, and inner diameter showed a downward trend around surgery, with statistically significant differences between each time point (< 0.01). The PVST incidence rate was 55.7%. Univariate analysis revealed that the platelet (PLT) levels on the postoperative 3rd and 7th days (= 0.001; < 0.001), D-dimer (D-D) on the postoperative 7th and 14th days (= 0.002; < 0.001), preoperative portal vein velocity, flow, diameter (< 0.001), and splenic vein diameter (< 0.001) were all statistically and significantly different between the two groups. Multivariate logistic regression revealed a significant increase in PLT on the postoperative 7th day (OR= 1.043, 95% CI, 1.027-1.060, < 0.001) and D-D on the postoperative 14th day (OR = 1.846, 95% CI, 1.400-2.435, < 0.001). Preoperative portal and splenic vein diameters (OR = 1.565, 95% CI, 1.213-2.019, = 0.001; OR = 1.671, 95% CI, 1.305-2.140, < 0.001) were the risk factors for PVST. However, preoperative portal vein velocity and flow (OR = 0.578, 95% CI, 0.409-0.818, = 0.002; OR= 0.987, 95% CI, 0.975-0.990, = 0.046) were protective factors for PVST. Logit was calculated using a logistic regression prediction model with a cut-off value of -0.32 and an area under receiver operating characteristic curve of 0.952 with 88.61% accuracy.

Conclusions: Splenectomy relieves portal hypertension by reducing the hemodynamics index. PVST is linked to multiple factors, including preoperative portal vein diameter, velocity, flow, and splenic vein diameter, especially PLT on the postoperative 7th day and D-D on the postoperative 14th day. The predictive model is accurate in predicting PVST.
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http://dx.doi.org/10.3389/fsurg.2022.834466DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9189385PMC
May 2022

Characteristics of the microbiota and metabolic profile of high-temperature Daqu with different grades.

World J Microbiol Biotechnol 2022 Jun 14;38(8):137. Epub 2022 Jun 14.

Key Laboratory of Precision Nutrition and Food Quality, Key Laboratory of Functional Dairy, Ministry of Education, Department of Nutrition and Health, China Agricultural University, Beijing, 100083, China.

The superior grade Daqu (S_Daqu) and normal grade Daqu (N_Daqu) have obvious differences in flavor, fracture surface, appearance, etc., which can be accurately grouped by well-trained panel based on their sensory properties. However, the differences in microbial community diversity and metabolites between the S_Daqu and N_Daqu were still unclear. The culture-dependent method, the third generation Pacific Biosciences (PacBio) single-molecule, real-time (SMRT) sequencing technology, and nuclear magnetic resonance (NMR) were combined to show the characteristics in microorganisms and metabolites. Results showed that the fungal counts were higher in N_Daqu while the richness of bacterial communities was higher in S_Daqu (P < 0.05). Lentibacillus, Burkholderia, Saccharopolyspora, Thermoascus, and Rasamsonia were the dominant genera of S_Daqu while Staphylococcus, Scopulibacillus, and Chromocleista were the dominant genera in N_Daqu. The content of differential acids, amino acids, and alcohols including fumarate, glucuronate, glycine, 4-carboxyglutamate, and myo-inositol in S_Daqu was higher than that in N_Daqu by H NMR coupled with multivariate statistical analysis. The network analysis regarding microbes and metabolites suggested that Saccharopolyspora showed a strong positive correlation with 4-carboxyglutamate while Thermoascus and Chromocleista were highly negatively correlated with alanine and isobutyrate, respectively. Linear Discriminant Analysis (LDA) Effect Size (LEfSe) revealed that Macrococcus and Caulobacter were regarded as bacterial biomarkers in the S_Daqu while Chromocleista was the key fungal genera in N_Daqu. Functionality prediction indicated that the bacteria in S_Daqu were largely involved in more metabolic activities including biosynthesis, degradation, detoxification, and generation of precursor metabolite and energy.
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http://dx.doi.org/10.1007/s11274-022-03303-7DOI Listing
June 2022

FORUM: Remote testing for psychological and physiological acoustics.

J Acoust Soc Am 2022 05;151(5):3116

VA Loma Linda Healthcare System, Loma Linda, California 92357, USA.

Acoustics research involving human participants typically takes place in specialized laboratory settings. Listening studies, for example, may present controlled sounds using calibrated transducers in sound-attenuating or anechoic chambers. In contrast, remote testing takes place outside of the laboratory in everyday settings (e.g., participants' homes). Remote testing could provide greater access to participants, larger sample sizes, and opportunities to characterize performance in typical listening environments at the cost of reduced control of environmental conditions, less precise calibration, and inconsistency in attentional state and/or response behaviors from relatively smaller sample sizes and unintuitive experimental tasks. The Acoustical Society of America Technical Committee on Psychological and Physiological Acoustics launched the Task Force on Remote Testing (https://tcppasa.org/remotetesting/) in May 2020 with goals of surveying approaches and platforms available to support remote testing and identifying challenges and considerations for prospective investigators. The results of this task force survey were made available online in the form of a set of Wiki pages and summarized in this report. This report outlines the state-of-the-art of remote testing in auditory-related research as of August 2021, which is based on the Wiki and a literature search of papers published in this area since 2020, and provides three case studies to demonstrate feasibility during practice.
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http://dx.doi.org/10.1121/10.0010422DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9305596PMC
May 2022

A 5'-tiRNA fragment that inhibits proliferation and migration of laryngeal squamous cell carcinoma by targeting PIK3CD.

Genomics 2022 May 26;114(4):110392. Epub 2022 May 26.

Department of Otorhinolaryngology Head and Neck Surgery, Ningbo Medical Center Lihuili Hospital, Ningbo, Zhejiang, China; Department of Otorhinolaryngology Head and Neck Surgery, Lihuili Hospital affiliated to Ningbo University, Ningbo, Zhejiang, China. Electronic address:

tRNA-derived small RNAs (tsRNAs) participate in several biological processes, including carcinogenesis. The correlations between tsRNAs and human cancers are attracting substantial attention. Nevertheless, the involvement of tsRNAs in laryngeal squamous cell carcinoma (LSCC) progression remains unclear. We constructed tsRNAs expression profiles in LSCC and adjacent normal tissues by next-generation sequencing. Interestingly, we identified a specific 5'-tiRNA fragment (tRF-33-Q1Q89P9L842205) that was significantly downregulated and was closely associated with lymph node metastasis and advanced stages of LSCC. Importantly, we found that tRF-33-Q1Q89P9L842205 suppressed cell growth, proliferation, migration, invasion and induced apoptosis in LSCC by directly silencing phosphoinositide 3-kinase catalytic subunit (PIK3CD). We speculated that tRF-33-Q1Q89P9L842205 is a potential diagnostic biomarker for LSCC and acts as a tumor suppressor by directly targeting PIK3CD.
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http://dx.doi.org/10.1016/j.ygeno.2022.110392DOI Listing
May 2022

[Safety Analysis of Perioperative Period in Non-Small Cell Lung Cancer Patients with Comorbid Pneumoconiosis].

Sichuan Da Xue Xue Bao Yi Xue Ban 2022 May;53(3):488-492

Department of Thoracic Surgery, West China Fourth Hospital, Sichuan University, Chengdu 610041, China.

Objective: To explore the surgical safety of patients with comorbid non-small cell lung cancer (NSCLC) and pneumoconiosis.

Methods: In this study, the clinical data of 165 NSCLC patients treated at West China Fourth Hospital, Sichuan University from August 2019 to May 2021 were collected. Among them, 21 patients with comorbid pneumoconiosis were included in the pneumoconiosis group, and the remaining 144 patients were included in the general group. Radical resection for lung cancer was performed in both groups. The perioperative clinical data, including preoperative, intraoperative and postoperative indicators, of the two groups were compared and analyzed.

Results: There was no perioperative death in either group. The proportions of male patients and patients with smoking history in the pneumoconiosis group were significantly higher than those in the general group ( <0.05). The body mass index (BMI), pulmonary ventilation function and diffusion function in the pneumoconiosis group were significantly lower than those in the general group ( <0.05). There was no significant difference in the median operative time and the median volume of intraoperative blood loss between the pneumoconiosis group and the general group. In the pneumoconiosis group, the proportion of advanced tumors (stage Ⅱ/Ⅲ), incidence of postoperative complications, median duration of postoperative intubation, and postoperative length of hospital stay were higher/longer than those of the normal group ( <0.05). Compared with the general group, the incidences of lymph node calcification, dense pleural adhesion and surgical method alteration (switching from thoracoscopic surgery to open surgery or video-assisted thoracoscopy) were also significantly higher in the pneumoconiosis group ( <0.05). Univariate analysis showed that age, smoking history, pneumoconiosis, pulmonary ventilation dysfunction, lymph node calcification, dense pleural adhesion and the volume of intraoperative blood loss were the risk factors for postoperative complications. Further multivariate regression analysis demonstrated that smoking history ( =1.37, <0.05), lymph node calcification ( =2.36, <0.05) and pulmonary ventilation dysfunction ( =5.21, <0.05) were independent risk factors for postoperative complications.

Conclusion: NSCLC patients with comorbid pneumoconiosis face relatively greater risks during the perioperative period when they undergo radical resection for lung cancer. Therefore, the close attention of surgeons and the nursing staff should be raised accordingly.
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http://dx.doi.org/10.12182/20220560108DOI Listing
May 2022
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