Publications by authors named "Yi Ren"

757 Publications

Fibrotic Scar in CNS Injuries: From the Cellular Origins of Fibroblasts to the Molecular Processes of Fibrotic Scar Formation.

Cells 2022 Aug 2;11(15). Epub 2022 Aug 2.

Department of Biomedical Sciences, Florida State University College of Medicine, Tallahassee, FL 32304, USA.

Central nervous system (CNS) trauma activates a persistent repair response that leads to fibrotic scar formation within the lesion. This scarring is similar to other organ fibrosis in many ways; however, the unique features of the CNS differentiate it from other organs. In this review, we discuss fibrotic scar formation in CNS trauma, including the cellular origins of fibroblasts, the mechanism of fibrotic scar formation following an injury, as well as the implication of the fibrotic scar in CNS tissue remodeling and regeneration. While discussing the shared features of CNS fibrotic scar and fibrosis outside the CNS, we highlight their differences and discuss therapeutic targets that may enhance regeneration in the CNS.
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http://dx.doi.org/10.3390/cells11152371DOI Listing
August 2022

Characterization of the volatile flavor profiles of Zhenjiang aromatic vinegar combining a novel nanocomposite colorimetric sensor array with HS-SPME-GC/MS.

Food Res Int 2022 Sep 28;159:111585. Epub 2022 Jun 28.

School of Food and Biological Engineering, Jiangsu University, Xuefu Road 301, Zhenjiang 212013, Jiangsu, PR China.

In this work, the feasibility of combining headspace solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC/MS) with colorimetric sensor array (CSA) for flavor characterization of Zhenjiang aromatic vinegar (ZAV) from different grades was evaluated. Firstly, a new type of nanocomposite CSA that combines porous nanomaterials including hollow zeolitic imidazolate framework-8 (H-ZIF-8) and Universitetet i Oslo-66-NO (UiO-66-NO) with chemically responsive dyes was successfully constructed. Then, the nanocomposite CSA was applied to effectively discriminate ZAV of different grades and further quantitively predict the characteristic aroma components by using multivariate data analysis. Compared with other pattern recognition methods, support vector machine (SVM) model achieved the highest recognition rate both for training set (100%) and prediction set (94.44%). Furthermore, a good performance of quantitative prediction of characteristic aroma components of ZAV including acetic acid, total volatile acids, furfural, aldehyde ketones, ethyl acetate and esters combining CSA with partial least square (PLS) regression was achieved with all the correlation coefficients being over 0.80 for training and prediction sets. Therefore, the nanocomposite CSA combined with chemometrics could be an effective tool for the rapid and nondestructive assessment of flavor and quality of vinegar.
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http://dx.doi.org/10.1016/j.foodres.2022.111585DOI Listing
September 2022

Two-dimensional sound field reproduction based on Mathieu function expansion.

J Acoust Soc Am 2022 Jul;152(1):416

Graduate School of Informatics and Engineering, University of Electro-Communications, Chofu, Tokyo 182-8585, Japan.

In this study, a two-dimensional sound field reproduction method based on Mathieu function expansion (MFE) is proposed. Mathieu functions are orthogonal solutions of the Helmholtz equation in an elliptical coordinate system. A MFE, which is similar to the conventional circular harmonic expansion, is applied to the sound field. The MFE-based method introduces elliptical properties such that the listening area is an ellipse. Three methods are described: an analytical sound field reproduction method for elliptical loudspeaker arrays, a method applying transformation (i.e., stretch, rotation, and translation) to the listening area, and a numerical approach for arbitrarily shaped arrays. A suitable truncation order for the MFE is also derived. The performance of all methods is tested in computer simulations with examples.
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http://dx.doi.org/10.1121/10.0012687DOI Listing
July 2022

Vapor-Phase Infiltration of Polymer of Intrinsic Microporosity 1 (PIM-1) with Trimethylaluminum (TMA) and Water: A Combined Computational and Experimental Study.

J Phys Chem B 2022 Aug 3;126(31):5920-5930. Epub 2022 Aug 3.

School of Materials Science and Engineering, Georgia Institute of Technology, 771 Ferst Drive NW, Atlanta, Georgia 30332, United States.

Vapor-phase infiltration, a postpolymerization modification process, has demonstrated the ability to create organic-inorganic hybrid membranes with excellent stability in organic solvents while maintaining critical membrane properties of high permeability and selectivity. However, the chemical reaction pathways that occur during VPI and their implications on the hybrid membrane stability are poorly understood. This paper combines quartz crystal microbalance gravimetry (QCM) and chemical characterization with first-principles simulations at the atomic scale to study each processing step in the infiltration of polymer of intrinsic microporosity 1 (PIM-1) with trimethylaluminum (TMA) and its co-reaction with water vapor. Building upon results from QCM experiments and SEM/EDX, which find TMA remains within PIM-1 even under long desorption times, density functional theory (DFT) simulations identify that an energetically stable coordination forms between the metal-organic precursor and PIM-1's nitrile functional group during the precursor exposure step of VPI. In the subsequent water vapor exposure step, the system undergoes a series of exothermic reactions to form the final hybrid membrane. DFT simulations indicate that these reaction pathways result in aluminum oxyhydroxide species consistent with XPS and FTIR characterization. Both NMR and DFT simulations suggest that the final aluminum structure is primarily 6-fold coordinated and that the aluminum is at least dimerized, if not further "polymerized". According to the simulations, coordination of the aluminum with at least one nitrile group from the PIM-1 appears to weaken significantly as the final inorganic structure emerges but remains present to enable the formation of the 6-fold coordination species. Water molecules are proposed to complete the coordination complex without further increasing the aluminum's oxidation state. This study provides new insights into the infiltration process and the chemical structure of the final hybrid membrane including support for the possible mechanism of solvent stability.
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http://dx.doi.org/10.1021/acs.jpcb.2c01928DOI Listing
August 2022

Novel potent liposome agonists of triggering receptor expressed on myeloid cells 2 phenocopy antibody treatment in cells.

Glia 2022 Aug 1. Epub 2022 Aug 1.

Rare and Neurology TA, Sanofi, Chilly-Mazarin, France.

The receptor Triggering Receptor Expressed on Myeloid cells 2 (TREM2) is associated with several neurodegenerative diseases including Alzheimer's Disease and TREM2 stimulation represents a novel therapeutic opportunity. TREM2 can be activated by antibodies targeting the stalk region, most likely through receptor dimerization. Endogenous ligands of TREM2 are suggested to be negatively charged apoptotic bodies, mimicked by phosphatidylserine incorporated in liposomes and other polyanionic molecules likely binding to TREM2 IgV fold. However, there has been much discrepancy in the literature on the nature of phospholipids (PLs) that can activate TREM2 and on the stability of the corresponding liposomes over time. We describe optimized liposomes as robust agonists selective for TREM2 over TREM1 in cellular system. The detailed structure/activity relationship studies of lipid polar heads indicate that negatively charged lipid heads are required for activity and we identified the shortest maximally active PL sidechain. Optimized liposomes are active on both TREM2 common variant and TREM2 R47H mutant. Activity and selectivity were further confirmed in different native TREM2 expressing cell types including on integrated cellular responses such as stimulation of phagocytic activity. Such tool agonists will be useful in further studies of TREM2 biology in cellular systems alongside antibodies, and in the design of small molecule synthetic TREM2 agonists.
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http://dx.doi.org/10.1002/glia.24252DOI Listing
August 2022

Oncolytic viruses combined with immune checkpoint therapy for colorectal cancer is a promising treatment option.

Front Immunol 2022 15;13:961796. Epub 2022 Jul 15.

Tsinghua-Peking Joint Center for Life Sciences, School of Medicine, Tsinghua University, Beijing, China.

Immunotherapy is one of the promising strategies in the treatment of oncology. Immune checkpoint inhibitors, as a type of immunotherapy, have no significant efficacy in the clinical treatment of patients with pMMR/MSS/MSI-L mCRC alone. Therefore, there is an urgent need to find combination therapies that can improve the response rate of immune checkpoint inhibitors. Oncolytic viruses are a new class of cancer drugs that, in addition to directly lysing tumor cells, can facilitate the action of immune checkpoint inhibitors by modulating the tumor microenvironment and transforming "cold" tumors into "hot" ones. The combination of oncolytic viruses and immune checkpoint inhibitors is currently being used in several primary and clinical studies to treat tumors with exciting results. The combination of genetically modified "armed" OV with ICIs is expected to be one of the treatment options for pMMR/MSS/MSI-L mCRC. In this paper, we will analyze the current status of oncolytic viruses and ICIs available for the treatment of CRC. The feasibility of OV in combination with ICI for CRC will be discussed in terms of the mechanism of action of OV in treating tumors.
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http://dx.doi.org/10.3389/fimmu.2022.961796DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334725PMC
August 2022

The Emerging Roles of Rad51 in Cancer and Its Potential as a Therapeutic Target.

Front Oncol 2022 7;12:935593. Epub 2022 Jul 7.

Department of Thoracic Surgery, Shenyang Chest Hospital & Tenth People's Hospital, Shenyang, China.

Defects in DNA repair pathways are emerging hallmarks of cancer. Accurate DNA repairs and replications are essential for genomic stability. Cancer cells require residual DNA repair capabilities to repair the damage from replication stress and genotoxic anti-tumor agents. Defective DNA repair also promotes the accumulation of genomic changes that eventually lead to tumorigenesis, tumor progression, and therapeutic resistance to DNA-damaging anti-tumor agents. Rad51 recombinase is a critical effector of homologous recombination, which is an essential DNA repair mechanism for double-strand breaks. Rad51 has been found to be upregulated in many malignant solid tumors, and is correlated with poor prognosis. In multiple tumor types, Rad51 is critical for tumor metabolism, metastasis and drug resistance. Herein, we initially introduced the structure, expression pattern of Rad51 and key Rad51 mediators involved in homologous recombination. Additionally, we primarily discussed the role of Rad51 in tumor metabolism, metastasis, resistance to chemotherapeutic agents and poly-ADP ribose polymerase inhibitors.
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http://dx.doi.org/10.3389/fonc.2022.935593DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9300834PMC
July 2022

A mechanistic review on aerobic denitrification for nitrogen removal in water treatment.

Sci Total Environ 2022 Jul 19;847:157452. Epub 2022 Jul 19.

School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China; Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.

The traditional biological nitrogen removal technology consists of two steps: nitrification by autotrophs in aerobic circumstances and denitrification by heterotrophs in anaerobic situations; however, this technology requires a huge area and stringent environmental conditions. Researchers reached the conclusion that the denitrification process could also be carried out in aerobic circumstances with the discovery of aerobic denitrification. The aerobic denitrification process is carried out by aerobic denitrifying bacteria (ADB), most of which are heterotrophic bacteria that can metabolize various forms of nitrogen compounds under aerobic conditions and directly convert ammonia nitrogen to N for discharge from the system. Despite the fact that there is no universal agreement on the mechanism of aerobic denitrification, this article reviewed four current explanations for the denitrification mechanism of ADB, including the microenvironment theory, theory of enzyme, electron transport bottlenecks theory, and omics study, and summarized the parameters affecting the denitrification efficiency of ADB in terms of carbon source, temperature, dissolved oxygen (DO), and pH. It also discussed the current status of the application of aerobic denitrification in practical processes. Following the review, the difficulties of present aerobic denitrification technology are outlined and future research options are highlighted. This review may help to improve the design of current wastewater treatment facilities by utilizing ADB for effective nitrogen removal and provide the engineers with relevant references.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157452DOI Listing
July 2022

Performance of TB-LAMP in the Diagnosis of Tuberculous Empyema Using Samples Obtained From Pleural Decortication.

Front Med (Lausanne) 2022 29;9:879772. Epub 2022 Jun 29.

Department of Tuberculosis, Shenyang Tenth People's Hospital, Shenyang Chest Hospital, Shenyang, China.

Purpose: To evaluate the performance of TB-LAMP in the diagnosis of TB empyema using pleural tissue specimens obtained during pleural decortication.

Methods: Using the clinical records and the different diagnostic test results of patients who underwent pleural decortication in a TB-designated hospital over 3.5 years, we calculated the sensitivity, specificity positive predictive, and negative predictive values of the pathology, MGIT 960 culture, and TB-LAMP obtained by using pleural tissue specimens against the etiologic diagnosis and composite clinical reference standard (CCRS) as the reference standards.

Result: A total of 304 patients' records were extracted. All these patients had gone through pleural decortication. When the etiologic diagnosis was used as the reference, the sensitivity of TB-LAMP in identifying TB empyema was 77.8% (compared to 10.6% of MGIT 960 < 0.05). The sensitivity of MGIT 960, pathology, and TB-LAMP was 8.2%, 77.7%, and 67.2% against CCRS as the reference; and the specificity of the three was 100.0, 100.0, and 96.2% against the same standard. A combination of pathology and TB-LAMP would increase the sensitivity and specificity to 84.7 and 96.0%. Using TB-LAMP to diagnose TB empyema using pleural tissue samples obtained from pleural decortication was faster with satisfactory performance.

Conclusion: TB-LAMP has great potential in faster and more accurate diagnosis of TB empyema. Our findings provide insights for optimizing diagnostic algorithms for TB empyema.
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http://dx.doi.org/10.3389/fmed.2022.879772DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9278273PMC
June 2022

Positive affect decreases the negative association between social mobility belief and physical health among Chinese rural-to-urban migrant adolescents.

Psychoneuroendocrinology 2022 Sep 22;143:105846. Epub 2022 Jun 22.

Institute of Developmental Psychology, Faculty of Psychology, Beijing Normal University, Beijing, China; Beijing Key Laboratory of Applied Experimental Psychology, National Demonstration Center for Experimental Psychology Education, Beijing Normal University, China. Electronic address:

Objective: The belief in upward social mobility promotes the mental health and social adaptation of disadvantaged adolescents. However, efforts for upward mobility may facilitate psychosocial adaptation while undermining physical health for minority youth from disadvantaged backgrounds. Therefore, we aimed to examine how social mobility belief is associated with mental and physical health among Chinese rural-to-urban migrant adolescents and explore whether positive affect acts as a protective factor moderating these relationships.

Method: A total of 158 rural-to-urban migrant adolescents aged 10-14 completed a self-report questionnaire and underwent medical examination.

Results: Social mobility belief and positive affect were positively related to mental health (general self-efficacy). Positive affect interacted with social mobility belief in predicting physical health (self-rated health and allostatic load). For migrant adolescents with low levels of positive affect, strong belief in social mobility was associated with poor self-rated health and high allostatic load. For migrant adolescents with high levels of positive affect, their physical health was better and not associated with social mobility belief.

Conclusions: Social mobility belief showed a double-edged sword effect; it was positively associated with mental adaptation while negatively associated with physical health among Chinese rural-to-urban migrant adolescents. Positive affect acted as a protective factor for decreasing the negative association between social mobility belief and physical health.
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http://dx.doi.org/10.1016/j.psyneuen.2022.105846DOI Listing
September 2022

Fabrication and evaluation of chitosan modified filter paper for chlorpyrifos detection in wheat by surface-enhanced Raman spectroscopy.

J Sci Food Agric 2022 Jun 29. Epub 2022 Jun 29.

School of Food and Biological Engineering, Jiangsu University, Zhenjiang, China.

Background: Chlorpyrifos is a commonly used organophosphorus pesticide in agriculture. However, its neurotoxicity poses a huge threat to human health. In the present study, a chitosan-modified filter paper-based surface enhanced Raman scattering active substrate (Ch/AgNPs/paper) was fabricated and used to detect trace amounts of chlorpyrifos in 120 treated wheat samples.

Results: Results showed that the Ch/AgNPs/paper substrate could be used to enhance the chlorpyrifos spectral fingerprint only up to a concentration of 0.000558 mg L . Following Raman spectra acquisition, three pre-processing methods, including Savitzky-Golay (Savitsky-Golay filter with a second order polynomial) smoothing with first derivative and second derivative and normalization, were used to reduce baseline variation and increase resolutions of spectral peak features of the original spectra dataset. Then, prediction models based on partial least squares were established for detecting chlorpyrifos pesticide residue in wheat. The partial least squares model with normalization yielded optimal result, with a correlation coefficient of 0.9764, root mean square error of prediction of 1.22 mg L in the prediction, and relative analysis deviation of 4.12. Five unknown samples were prepared to verify the accuracy of the prediction model. The predicted recoveries were calculated to be between 97.25% and 119.38% with an absolute t value of 0.598. The value of a t-test shows that the prediction model is accurate and reliable.

Conclusion: The present study demonstrates that the proposed method can achieve rapid detection of chlorpyrifos in wheat. © 2022 Society of Chemical Industry.
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http://dx.doi.org/10.1002/jsfa.12098DOI Listing
June 2022

The diversity of elaborate petals in Isopyreae (Ranunculaceae): a special focus on nectary structure.

Protoplasma 2022 Jun 27. Epub 2022 Jun 27.

Key Laboratory of Medicinal Plant Resource and Natural Pharmaceutical Chemistry of Ministry of Education, Shaanxi Normal University, Xi'an, 710062, China.

Elaborate petals are highly diverse in morphology, structure, and epidermal differentiation and play a key role in attracting pollinators. There have been few studies on the elaborate structure of petals in the tribe Isopyreae (Ranunculaceae). Seven genera in Isopyreae (Aquilegia, Semiaquilegia, Urophysa, Isopyrum, Paraquilegia, Dichocarpum, and Leptopyrum) have petals that vary in morphology, and two genera (Enemion and Thalictrum) have no petals. The petals of nine species belonged to 7 genera in the tribe were studied to reveal their nectary structure, epidermal micromorphology and ancestral traits. The petal nectaries of Isopyreae examined in this study were located at the tip of spurs (Aquilegia yabeana and A. rockii), or the bottom of shallow sacs (Semiaquilegia adoxoides, Urophysa henryi, Isopyrum manshuricum, and Paraquilegia microphylla), a cup-shaped structure (Dichocarpum fargesii) and a bilabiate structure (Leptopyrum fumarioides). The petal nectary of eight species in Isopyreae (except A. ecalcarata) was composed of secretory epidermis, nectary parenchyma, and vascular tissues, and some sieve tubes reached the secretory parenchyma cells. Among the eight species with nectaries examined in the present study, A. yabeana had the most developed nectaries, with 10-15 layers of secretory parenchyma cells. The epidermal cells of mature petals of the nine species were divided into 11 types. Among these 11 types, there were two types of secretory cells and two types of trichomes. Aquilegia yabeana and A. rockii had the highest number of cell types (eight types), and I. manshuricum and L. fumarioides had the lowest number of cell types (three types). Aquilegia ecalcarata had no secretory cells, and the papillose conical polygonal secretory cells of D. fargesii were different from those of the other seven species with nectaries. Trichomes were found only in Aquilegia, Semiaquilegia, Urophysa, and Paraquilegia. The ancestral mode of nectar presentation in Isopyreae was petals with hidden nectar (70.58%). The different modes of nectar presentation in petals may reflect adaptations to different pollinators in Isopyreae.
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http://dx.doi.org/10.1007/s00709-022-01787-6DOI Listing
June 2022

Ultramicroporous Organophosphorus Polymers via Self-Accelerating P-C Coupling Reactions: Kinetic Effects on Crosslinking Environments and Porous Structures.

J Am Chem Soc 2022 Jul 23;144(26):11748-11756. Epub 2022 Jun 23.

School of Physical Science and Technology, ShanghaiTech University, Shanghai 201210, People's Republic of China.

Porous organic polymers (POPs) have drawn significant attention in diverse applications. However, factors affecting the heterogeneous polymerization and porosity of POPs are still not well understood. Herein, we report a new strategy to construct porous organophosphorus polymers (POPPs) with high surface areas (1283 m/g) and ultramicroporous structures (0.67 nm). The strategy harnesses an efficient transition-metal-catalyzed phosphorus-carbon (P-C) coupling reaction at the trigonal pyramidal P-center, which is distinct from the typical carbon-carbon coupling reaction utilized in the synthesis of POPs. As the first kinetic study on the coupling reaction of POPs, we uncovered a self-accelerating reaction characteristic, which is controlled by the choice of bases and catalysts. The self-accelerating characteristic of the P-C coupling reaction is beneficial for the high surface area and uniform ultramicroporosity of POPPs. The direct crosslinking of the P-centers allows P solid-state (ss)NMR experiments to unambiguously reveal the crosslinking environments of POPPs. Leveraging on the kinetic studies and P ssNMR studies, we were able to reveal the kinetic effects of the P-C coupling reaction on both the crosslinking environments and the porous structures of POPPs. Furthermore, our studies show that the CO uptake capacity of POPPs is highly dependent on their porous structures. Overall, our studies paves the way to design new POPs with better controlled chemical and ultramicroporous structures, which have potential applications for CO capture and separation.
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http://dx.doi.org/10.1021/jacs.2c03759DOI Listing
July 2022

Interference with connective tissue growth factor attenuated fibroblast-to-myofibroblast transition and pulmonary fibrosis.

Ann Transl Med 2022 May;10(10):566

Department of Emergency Medicine, Jinling Hospital, Medical School of Nanjing University, Nanjing, China.

Background: The aberrant activation and phenotype shift of resident fibroblasts in lung tissues via fibroblast-to-myofibroblast transition (FMT) is considered a pivotal step in pulmonary fibrogenesis, resulting in excessive extracellular matrix (ECM) production and deposition. However, the molecular mechanisms regulating FMT and lung fibrosis are still unclear. Connective tissue growth factor (CTGF) has been reported to be both an ECM protein and a versatile signaling molecule that is involved in multiple pathophysiological contexts, especially fibrosis. The relationship between CTGF, FMT, and lung fibrosis has not yet been well defined.

Methods: In this study, a pulmonary fibrosis (PF) rat model and FMT cell model induced by paraquat (PQ) were established to explore the relevant regulatory mechanisms and .

Results: The results showed that the CTGF was highly activated and was a mediator of canonical Wnt signaling during FMT and PF. The inhibition of the CTGF by small-interfering ribonucleic acid decreased the expression of FMT markers, including α-smooth muscle actin, vimentin, and collagen I, inhibited the activated Wnt signaling pathway, and ameliorated lung fibrosis.

Conclusions: Our findings showed that CTGF was the key effector of the FMT and fibrotic changes, and emphasized the therapeutic potential of the inhibitor or monoclonal antibody against CTGF for PF.
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http://dx.doi.org/10.21037/atm-22-1397DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201195PMC
May 2022

Prediction of Chlorophyll Content in Multi-Temporal Winter Wheat Based on Multispectral and Machine Learning.

Front Plant Sci 2022 27;13:896408. Epub 2022 May 27.

High-Quality Special Wheat Crop Engineering Technology Research Center, College of Agronomy, Xinjiang Agricultural University, Ũrũmqi, China.

To obtain the canopy chlorophyll content of winter wheat in a rapid and non-destructive high-throughput manner, the study was conducted on winter wheat in Xinjiang Manas Experimental Base in 2021, and the multispectral images of two water treatments' normal irrigation (NI) and drought stress (DS) in three key fertility stages (heading, flowering, and filling) of winter wheat were obtained by DJI P4M unmanned aerial vehicle (UAV). The flag leaf chlorophyll content (CC) data of different genotypes in the field were obtained by SPAD-502 Plus chlorophyll meter. Firstly, the CC distribution of different genotypes was studied, then, 13 vegetation indices, combined with the Random Forest algorithm and correlation evaluation of CC, and 14 vegetation indices were used for vegetation index preference. Finally, preferential vegetation indices and nine machine learning algorithms, Ridge regression with cross-validation (RidgeCV), Ridge, Adaboost Regression, Bagging_Regressor, K_Neighbor, Gradient_Boosting_Regressor, Random Forest, Support Vector Machine (SVM), and Least absolute shrinkage and selection operator (Lasso), were preferentially selected to construct the CC estimation models under two water treatments at three different fertility stages, which were evaluated by correlation coefficient (), root means square error (RMSE) and the normalized root mean square error (NRMSE) to select the optimal estimation model. The results showed that the CC values under normal irrigation were higher than those underwater limitation treatment at different fertility stages; several vegetation indices and CC values showed a highly significant correlation, with the highest correlation reaching.51; in the prediction model construction of CC values, different models under normal irrigation and water limitation treatment had high estimation accuracy, among which the model with the highest prediction accuracy under normal irrigation was at the heading stage. The highest precision of the model prediction under normal irrigation was in the RidgeCV model ( = 0.63, RMSE = 3.28, NRMSE = 16.2%) and the highest precision of the model prediction under water limitation treatment was in the SVM model ( = 0.63, RMSE = 3.47, NRMSE = 19.2%).
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http://dx.doi.org/10.3389/fpls.2022.896408DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9197342PMC
May 2022

Identification of Biomarkers Associated With CD4 T-Cell Infiltration With Gene Coexpression Network in Dermatomyositis.

Front Immunol 2022 30;13:854848. Epub 2022 May 30.

Department of Pediatrics, The Second Xiangya Hospital of Central South University, Changsha, China.

Background: Dermatomyositis is an autoimmune disease characterized by damage to the skin and muscles. CD4 T cells are of crucial importance in the occurrence and development of dermatomyositis (DM). However, there are few bioinformatics studies on potential pathogenic genes and immune cell infiltration of DM. Therefore, this study intended to explore CD4 T-cell infiltration-associated key genes in DM and construct a new model to predict the level of CD4 T-cell infiltration in DM.

Methods: GSE46239, GSE142807, GSE1551, and GSE193276 datasets were downloaded. The WGCNA and CIBERSORT algorithms were performed to identify the most correlated gene module with CD4 T cells. Matascape was used for GO enrichment and KEGG pathway analysis of the key gene module. LASSO regression analysis was used to identify the key genes and construct the prediction model. The correlation between the key genes and CD4 T-cell infiltration was investigated. GSEA was performed to research the underlying signaling pathways of the key genes. The key gene-correlated transcription factors were identified through the RcisTarget and Gene-motif rankings databases. The miRcode and DIANA-LncBase databases were used to build the lncRNA-miRNA-mRNA network.

Results: In the brown module, 5 key genes (chromosome 1 open reading frame 106 (), component of oligomeric Golgi complex 8 (), envoplakin (), GTPases of immunity-associated protein family member 6 (), and interferon-alpha inducible protein 6 ()) highly associated with CD4 T-cell infiltration were identified. The prediction model was constructed and showed better predictive performance in the training set, and this satisfactory model performance was validated in another skin biopsy dataset and a muscle biopsy dataset. The expression levels of the key genes promoted the CD4 T-cell infiltration. GSEA results revealed that the key genes were remarkably enriched in many immunity-associated pathways, such as JAK/STAT signaling pathway. The cisbp_M2205, transcription factor-binding site, was enriched in , , and . Finally, 3,835 lncRNAs and 52 miRNAs significantly correlated with key genes were used to build a ceRNA network.

Conclusion: The , , , , and genes are associated with CD4 T-cell infiltration. The prediction model constructed based on the 5 key genes may better predict the level of CD4 T-cell infiltration in damaged muscle and lesional skin of DM. These key genes could be recognized as potential biomarkers and immunotherapeutic targets of DM.
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http://dx.doi.org/10.3389/fimmu.2022.854848DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9196312PMC
May 2022

Evaluating immune response and metabolic related biomarkers pre-allogenic hematopoietic stem cell transplant in acute myeloid leukemia.

PLoS One 2022 14;17(6):e0268963. Epub 2022 Jun 14.

Division of Hematologic Malignancies and Cellular Therapy, Duke University School of Medicine, Durham, North Carolina, United States of America.

Although hematopoietic stem cell transplantation (HCT) is the only curative treatment for acute myeloid leukemia (AML), it is associated with significant treatment related morbidity and mortality. There is great need for predictive biomarkers associated with overall survival (OS) and clinical outcomes. We hypothesized that circulating metabolic, inflammatory, and immune molecules have potential as predictive biomarkers for AML patients who receive HCT treatment. This retrospective study was designed with an exploratory approach to comprehensively characterize immune, inflammatory, and metabolomic biomarkers. We identified patients with AML who underwent HCT and had existing baseline plasma samples. Using those samples (n = 34), we studied 65 blood based metabolomic and 61 immune/inflammatory related biomarkers, comparing patients with either long-term OS (≥ 3 years) or short-term OS (OS ≤ 1 years). We also compared the immune/inflammatory response and metabolomic biomarkers in younger vs. older AML patients (≤30 years vs. ≥ 55 years old). In addition, the biomarker profiles were analyzed for their association with clinical outcomes, namely OS, chronic graft versus host disease (cGVHD), acute graft versus host disease (aGVHD), infection and relapse. Several baseline biomarkers were elevated in older versus younger patients, and baseline levels were lower for three markers (IL13, SAA, CRP) in patients with OS ≥ 3 years. We also identified immune/inflammatory response markers associated with aGVHD (IL-9, Eotaxin-3), cGVHD (Flt-1), infection (D-dimer), or relapse (IL-17D, bFGF, Eotaxin-3). Evaluation of metabolic markers demonstrated higher baseline levels of medium- and long-chain acylcarnitines (AC) in older patients, association with aGVHD (lactate, long-chain AC), and cGVHD (medium-chain AC). These differentially expressed profiles merit further evaluation as predictive biomarkers.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0268963PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9197059PMC
June 2022

Mitoquinone mitigates paraquat-induced A549 lung epithelial cell injury by promoting MFN1/MFN2-mediated mitochondrial fusion.

J Biochem Mol Toxicol 2022 Jun 9:e23127. Epub 2022 Jun 9.

Department of Emergency Medicine, Jinling Hospital, Medical School of Nanjing University, Nanjing, PR China.

Paraquat (PQ) poisoning often leads to severe lung injuries, in which the mitochondria damage plays a critical role. Mitoquinone (MitoQ), a newly designed mitochondria-targeted antioxidant, has been proved for its benefit in mitochondria protection. However, the role of MitoQ in PQ-induced lung injury remains unclear. Thus, this study was performed to investigate the effect of MitoQ on PQ-induced lung injury and its underlying mechanisms. Our work showed that PQ caused the inhibition of A549 lung epithelial cell viability in a dose-dependent manner, while MitoQ remarkably mitigated the PQ-induced cell viability suppression. Besides this, PQ-mediated apoptosis of A549 cells was significantly attenuated by MitoQ, as indicated by the TUNEL assay and mitochondria membrane potential assay. Moreover, the intracellular reactive oxygen species (ROS) production was also dramatically suppressed when cotreated MitoQ with PQ. This could be ascribed to enhanced mitochondrial fusion mediated by Mitofusin 1 (MFN1)/Mitofusin 2 (MFN2), because MitoQ preserved mitochondrial network integrity, as reflected by MitoTracker staining, and MitoQ also increased the expression of MFN1/MFN2 in A549 cells after PQ treatment. Our data suggested MitoQ mitigated PQ-induced lung epithelial cell injury by promoting MFN1/MFN2-mediated mitochondrial fusion, and MitoQ might be a potential candidate drug for the treatment of PQ-induced lung injury.
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http://dx.doi.org/10.1002/jbt.23127DOI Listing
June 2022

A Proprioceptive Soft Robot Module Based on Supercoiled Polymer Artificial Muscle Strings.

Polymers (Basel) 2022 Jun 1;14(11). Epub 2022 Jun 1.

Department of Mechanical and Aerospace Engineering, The Hong Kong University of Science and Technology, Hong Kong, China.

In this paper, a multi-functional soft robot module that can be used to constitute a variety of soft robots is proposed. The body of the soft robot module made of rubber is in the shape of a long strip, with cylindrical chambers at both the top end and bottom end of the module for the function of actuators and sensors. The soft robot module is driven by supercoiled polymer artificial muscle (SCPAM) strings, which are made from conductive nylon sewing threads. Artificial muscle strings are embedded in the chambers of the module to control its deformation. In addition, SCPAM strings are also used for the robot module's sensing based on the linear relationship between the string's length and their resistance. The bending deformation of the robot is measured by the continuous change of the sensor's resistance during the deformation of the module. Prototypes of an inchworm-like crawling robot and a soft robotic gripper are made, whose crawling ability and grasping ability are tested, respectively. We envision that the proposed proprioceptive soft robot module could potentially be used in other robotic applications, such as continuum robotic arm or underwater robot.
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http://dx.doi.org/10.3390/polym14112265DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9182732PMC
June 2022

Phosphatidylserine-Specific Phospholipase A1 Alleviates Lipopolysaccharide-Induced Macrophage Inflammation by Inhibiting MAPKs Activation.

Biol Pharm Bull 2022 Aug 31;45(8):1061-1068. Epub 2022 May 31.

Department of Emergency Medicine, Jinling Clinical Medical College of Nanjing Medical University.

Macrophages are a key in innate immune responses and play vital roles in homeostasis and inflammatory diseases. Phosphatidylserine-specific phospholipase A1 (PS-PLA1) is a specific phospholipase which hydrolyzes fatty acid from the sn-1 position of phosphatidylserine (PS) to produce lysophosphatidylserine (lysoPS). Both PS and lysoPS are associated with activation of immune cells including macrophages. However, the effect of PS-PLA1 on macrophage inflammation remains unclear. The purpose of this study is to evaluate the role of PS-PLA1 in lipopolysaccharide (LPS)-induced macrophage inflammation. Alterations of PS-PLA1 expression in LPS-stimulated RAW264.7 macrophages were investigated via Western blot. PS-PLA1 stable knockdown and overexpression RAW264.7 cell lines were generated by infecting cells with appropriate lentiviral vectors, respectively. PS-PLA1 expression was found to be dramatically upregulated in RAW264.7 macrophages after LPS stimulation. PS-PLA1 knockdown promotes while PS-PLA1 overexpression ameliorates the release of tumor necrosis factor (TNF)-α, interleukin (IL)-1β and nitric oxide from RAW264.7 cells and M1 macrophage polarization. Additionally, PS-PLA1 knockdown facilitates phosphorylation of p38, extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK), while PS-PLA1 overexpression attenuates their phosphorylation. Moreover, mitogen-activated protein kinase (MAPK) inhibitors block the release of TNF-α and IL-1β in PS-PLA1 knockdown RAW264.7 cells after LPS stimulation. These findings suggest PS-PLA1 ameliorates LPS-induced macrophage inflammation by inhibiting MAPKs activation, and PS-PLA1 might be considered as a target for modulating macrophage inflammation.
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http://dx.doi.org/10.1248/bpb.b22-00001DOI Listing
August 2022

Effect of High-Temperature Hydrothermal Treatment on the Cellulose Derived from the Plant.

Polymers (Basel) 2022 May 18;14(10). Epub 2022 May 18.

College of Furniture and Art Design, Central South University of Forestry and Technology, Changsha 410004, China.

Cellulose has attracted considerable attention as the most promising potential candidate raw material for the production of bio-based polymeric materials. In the last decade, significant progress has been made in the production of biopolymers based on different cellulose forms. In this study, cellulose was obtained in an innovative and environmentally friendly way, using boxwood powder. Crude cellulose was obtained by treating powder with an ethanol-acetic acid-water mixture. Refined cellulose was then obtained by treatment with an acidic sodium hypochlorite solution and alkaline hydrogen peroxide solution. The novel chemistry of cellulose prepared by this method promises to be not only green, but also highly desirable, because of its lower emissions and low cost. It is crucial for the future of the global polymer industry. The refined cellulose was subjected to a high-temperature hydrothermal treatment under two temperatures and time conditions, with temperature gradients of 120, 140, and 160 °C, and time gradients of 1, 2, and 3 h. The samples were subjected to infrared and thermogravimetric analyses. The cellulose undergoes dehydration and thermal degradation reactions during the heat treatment process, and the thermal stability of the residual is enhanced, compared with that of virgin cellulose. Between 120 and 140 °C, the hydroxyl and hypomethyl groups on the surface of cellulose are shed. Groups in the amorphous region of the polymer are the first to be shed. The dehydration reaction reduces the number of free hydroxyl groups on the surface of the cellulose molecules. The dehydration reaction was accelerated by an increase in temperature. Between 140 and 160 °C, the β-(1,4)-glycosidic bond begins to slowly break and some furans are generated. The structure of cellulose undergoes reorganization during thermal treatment. The thermal stability of the modified material is greater than that of untreated cellulose.
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http://dx.doi.org/10.3390/polym14102053DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9143544PMC
May 2022

Serum and Synovial Markers in Patients with Rheumatoid Arthritis and Periprosthetic Joint Infection.

J Pers Med 2022 May 17;12(5). Epub 2022 May 17.

Center for Musculoskeletal Surgery, Clinic for Orthopedics, Charité University Hospital, 10117 Berlin, Germany.

Current diagnostic standards for PJI rely on inflammatory markers that are typically elevated in autoimmune diseases, thus making the diagnosis of PJI in patients with rheumatoid arthritis and joint replacement particularly complicated. There is a paucity of data on differentiating PJI from rheumatoid arthritis in patients with previous arthroplasty. In this study, we retrospectively analyzed the cases of 17 patients with rheumatoid arthritis and 121 patients without rheumatoid disease who underwent surgical intervention due to microbiology-positive PJI of the hip or knee joint. We assessed clinical patient characteristics, laboratory parameters, and prosthesis survival rates in patients with and without rheumatoid arthritis and acute or chronic PJI. ROC analysis was conducted for the analyzed parameters. In patients with chronic PJI, peripheral blood CRP ( = 0.05, AUC = 0.71), synovial WBC count ( = 0.02, AUC = 0.78), synovial monocyte cell count ( = 0.04, AUC = 0.75), and synovial PMN cell count ( = 0.02, AUC = 0.80) were significantly elevated in patients with rheumatoid arthritis showing acceptable to excellent discrimination. All analyzed parameters showed no significant differences and poor discrimination for patients with acute PJI. Median prosthesis survival time was significantly shorter in patients with rheumatoid arthritis ( = 0.05). In conclusion, routinely used laboratory markers have limited utility in distinguishing acute PJI in rheumatoid patients. In cases with suspected chronic PJI but low levels of serum CRP and synovial cell markers, physicians should consider the possibility of activated autoimmune arthritis.
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http://dx.doi.org/10.3390/jpm12050810DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9148028PMC
May 2022

Study on seafloor hydrothermal systems circulation flow and heat transfer characteristics.

Math Biosci Eng 2022 Apr;19(6):6186-6203

College of Power and Energy Engineering, Harbin Engineering University, Harbin, MO 150001, China.

In this work, the numerical simulation study of the hydrothermal flow and heat transfer process in the porous rock under 30 MPa pressure was developed. The flow and heat transfer characteristics of hydrothermal in rocks with different porosities are studied by changing the porosity of the rock. The simulation results show that the average flow velocity decreases and the average temperature increases when the porosity decreases. The velocity field and temperature field are coupled due to the nonlinear thermophysical properties of hydrothermal. The velocity field and temperature field have strongly interacted in the range of 400-450 ℃ and the effect of temperature on velocity is gradually diminishing outside the range. Most of the fluid will be "squeezed" into the crevice and the average velocity is almost three times the no-creviced case when a crevice is present. The existence of the crevice makes the total heat flux decrease from an overall perspective, and the crevice makes a large temperature gradient at the entrance and export of the crevice from a local perspective. These results provide theoretical support for the utilization of submarine hydrothermal fluid shallow circulation heat energy.
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http://dx.doi.org/10.3934/mbe.2022289DOI Listing
April 2022

Geriatric Assessment Reveals Actionable Impairments in Hematopoietic Stem Cell Transplantation Candidates Age 18 to 80 Years.

Transplant Cell Ther 2022 Aug 17;28(8):498.e1-498.e9. Epub 2022 May 17.

Division of Hematologic Malignancies and Cellular Therapy, Duke University Medical Center, Durham, North Carolina.

Allogeneic hematopoietic stem cell transplantation (HCT) is a potentially curative treatment for both malignant and nonmalignant hematologic diseases; however, reported rates of treatment-related mortality approach 30%. Outcomes are worse in patients who begin HCT with functional impairments. To detect such impairments, a geriatric assessment (GA) is recommended in adults age ≥65 years. Younger HCT candidates also may be impaired because of chemotherapy regimens pre-HCT. Therefore, we hypothesized that GA can be beneficial for adult patients of all ages and subsequently created a clinical pretransplantation optimization program to assess all HCT candidates using a modified GA. One-hundred fifty-seven patients were evaluated in 4 functional domains- physical, cognitive, nutritional, and psychological-at 2 time points prior to HCT-new patient evaluation (NPE) and sign-off (SO)-between October 2017 and January 2020. At NPE, 80.9% of the patients had at least 1 domain with a functional impairment, and physical (P = .006), cognitive (P = .04), and psychological (P = .04) impairments were associated with an increased likelihood of not proceeding to HCT. In addition, patients age 18 to 39 years were more likely than older patients to have a physical function impairment (P = .001). Between NPE and SO, 51.9% of the patients had resolution of 1 or more impairments, and nutritional impairment at SO was predictive of worse overall survival (P = .01). Our study shows that GA can identify functional impairments in patients of all ages. Early identification of impairments could facilitate referrals to supportive care and resolution of impairments prior to HCT, suggesting that GA could be recommended for HCT candidates of all ages.
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http://dx.doi.org/10.1016/j.jtct.2022.05.018DOI Listing
August 2022

A Digital Twin-Driven Life Prediction Method of Lithium-Ion Batteries Based on Adaptive Model Evolution.

Materials (Basel) 2022 May 6;15(9). Epub 2022 May 6.

School of Aeronautic Science and Engineering, Beihang University, Beijing 100191, China.

Accurate life prediction and reliability evaluation of lithium-ion batteries are of great significance for predictive maintenance. In the whole life cycle of a battery, the accurate description of the dynamic and stochastic characteristics of life has always been a key problem. In this paper, the concept of the digital twin is introduced, and a digital twin for reliability based on remaining useful cycle life prediction is proposed for lithium-ion batteries. The capacity degradation model, stochastic degradation model, life prediction, and reliability evaluation model are established to describe the randomness of battery degradation and the dispersion of the life of multiple cells. Based on the Bayesian algorithm, an adaptive evolution method for the model of the digital twin is proposed to improve prediction accuracy, followed by experimental verification. Finally, the life prediction, reliability evaluation, and predictive maintenance of the battery based on the digital twin are implemented. The results show the digital twin for reliability has good accuracy in the whole life cycle. The error can be controlled at about 5% with the adaptive evolution algorithm. For battery L1 and L6 in this case, predictive maintenance costs are expected to decrease by 62.0% and 52.5%, respectively.
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http://dx.doi.org/10.3390/ma15093331DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9103731PMC
May 2022

Analysis of Clinical and Genetic Characterization of Three Ataxia-Telangiectasia Pedigrees With Novel Gene Mutations.

Front Pediatr 2022 2;10:877826. Epub 2022 May 2.

Department of Pediatrics, The Second Xiangya Hospital of Central South University, Changsha, China.

Objective: The clinical manifestations of ataxia-telangiectasia (AT) are very complex and are easily misdiagnosed and missed. The purpose of this study was to explore the clinical characteristics and genetic features of five pediatric patients with AT from three pedigrees in china.

Methods: Retrospectively collected and analyzed the clinical data and genetic testing results of five AT patients diagnosed by the Whole-exome sequencing followed by Sanger sequencing. The five patients with AT were from three pedigrees, including two female patients (case 1 and case 2) in pedigree I, one male patient (case 3) in pedigree II, and two male patients (case 4 and case 5) in pedigree III. According to the United Kingdom Association for Clinical Genomic Science Best Practice Guidelines for Variants Classification in Rare Disease 2020 to grade the genetic variants.

Results: Five patients had mainly clinical presentations including unsteady gait, dysarthria, bulbar conjunctive telangiectasia, cerebellar atrophy, intellectual disability, stunted growth, increase of alpha-fetoprotein in serum, lymphopenia. Notably, one patient with classical AT presented dystonia as the first symptom. One patient had recurrent infections, five patients had serum Immunoglobulin (Ig) A deficiency, and two patients had IgG deficiency. In three pedigrees, we observed five pathogenic variants of the gene, which were c.1339C>T (p.Arg447Ter), c.7141_7151delAATGGAAAAAT (p.Asn2381GlufsTer18), c.437_440delTCAA (p.Leu146GlnfsTer6), c.2482A>T (p.Lys828Ter), and c.5495_5496+2delAAGT (p.Glu1832GlyfsTer4). Moreover, the c.437_440delTCAA, c.2482A>T, and c.5495_5496+2delAAGT were previously unreported variants.

Conclusions: Pediatric patients with classical AT may present dystonia as the main manifestation, or even a first symptom, besides typical cerebellar ataxia, bulbar conjunctive telangiectasia, etc. Crucially, we also found three novel pathogenic gene variants (c.437_440delTCAA, c.2482A>T, and c.5495_5496+2delAAGT), expanding the pathogenic gene mutation spectrum.
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http://dx.doi.org/10.3389/fped.2022.877826DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9108171PMC
May 2022

[Relationship between central line-associated bloodstream infection and catheter dwell time in neonates].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2022 Mar;34(3):320-324

Department of Neonatology, Xuzhou Central Hospital (the Xuzhou Clinical College of Xuzhou Medical University), Xuzhou 221009, Jiangsu, China. Corresponding author: Gao Xiangyu, Email:

The central line-associated bloodstream infection (CLABSI) is a common and serious complication of central venous catheterization, which can lead to various short-term and long-term adverse outcomes. Neonatal CLABSI did not increase linearly with the prolongation of peripherally inserted central catheter (PICC). When the umbilical venous catheter (UVC) is retained for more than 1-2 weeks, the incidence of neonatal CLABSI may increase significantly; PICC replacement at 7-10 days after UVC indwelling may reduce the incidence of CLABSI, but it has not been supported by evidence-based medicine. This article reviews the related studies between neonatal CLABSI and catheter dwell time, in order to plan the indwelling time more reasonably and prevent CLABSI better.
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http://dx.doi.org/10.3760/cma.j.cn121430-20211111-01688DOI Listing
March 2022

Natural variation in the NAC transcription factor NONRIPENING contributes to melon fruit ripening.

J Integr Plant Biol 2022 Jul 6;64(7):1448-1461. Epub 2022 Jun 6.

National Watermelon and Melon Improvement Center, Beijing Academy of Agricultural and Forestry Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Beijing Key Laboratory of Vegetable Germplasm Improvement, Beijing, 100097, China.

The NAC transcription factor NONRIPENING (NOR) is a master regulator of climacteric fruit ripening. Melon (Cucumis melo L.) has climacteric and non-climacteric fruit ripening varieties and is an ideal model to study fruit ripening. Two natural CmNAC-NOR variants, the climacteric haplotype CmNAC-NOR and the non-climacteric haplotype CmNAC-NOR , have effects on fruit ripening; however, their regulatory mechanisms have not been elucidated. Here, we report that a natural mutation in the transcriptional activation domain of CmNAC-NOR contributes to climacteric melon fruit ripening. CmNAC-NOR knockout in the climacteric-type melon cultivar "BYJH" completely inhibited fruit ripening, while ripening was delayed by 5-8 d in heterozygous cmnac-nor mutant fruits. CmNAC-NOR directly activated carotenoid, ethylene, and abscisic acid biosynthetic genes to promote fruit coloration and ripening. Furthermore, CmNAC-NOR mediated the transcription of the "CmNAC-NOR-CmNAC73-CmCWINV2" module to enhance flesh sweetness. The transcriptional activation activity of the climacteric haplotype CmNAC-NOR on these target genes was significantly higher than that of the non-climacteric haplotype CmNAC-NOR . Moreover, CmNAC-NOR complementation fully rescued the non-ripening phenotype of the tomato (Solanum lycopersicum) cr-nor mutant, while CmNAC-NOR did not. Our results provide insight into the molecular mechanism of climacteric and non-climacteric fruit ripening in melon.
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http://dx.doi.org/10.1111/jipb.13278DOI Listing
July 2022

Lobectomy versus segmentectomy in patients with stage T (> 2 cm and ≤ 3 cm) N0M0 non-small cell lung cancer: a propensity score matching study.

J Cardiothorac Surg 2022 May 11;17(1):110. Epub 2022 May 11.

Department of Thoracic Surgery, Shenyang Chest Hospital and Tenth People's Hospital, No. 11, Beihai Street, Dadong District, Shenyang, 110044, Liaoning, People's Republic of China.

Background: The safety and effectiveness of lung segmentectomy in patients with early non-small cell lung cancer (NSCLC) remains controversial. We have therefore reviewed the clinicopathologic characteristics and survival outcomes of patients treated with lobectomy or segmentectomy for early T (> 2 and ≤ 3 cm) N0M0 NSCLC.

Methods: We obtained data from the Surveillance, Epidemiology, and End Results database for patients who underwent lobectomy or segmentectomy between 2004 and 2015. To reduce bias and imbalances between the treatment groups, propensity score matching analysis was performed. We used Kaplan-Meier curves to estimate overall survival (OS) and lung cancer-specific survival (LCSS). We conducted univariate and multivariate Cox proportional hazards regression analyses to identify independent prognostic factors for OS and cancer-specific survival, and applied the Cox proportional hazards model to create forest plots.

Results: Before matching, both univariate and multivariate Cox regression analyses revealed that patients who underwent lobectomy exhibited better OS (P < 0.001) and LCSS (P = 0.001) than patients who underwent segmentectomy. However, after matching, survival differences between the groups were not significant; OS (P = 0.434) and LCSS (P = 0.593). Regression analyses revealed that age and tumor grade were independent predictors of OS and LCSS (P < 0.05).

Conclusions: Patients with stage T (> 2 and ≤ 3 cm) N0M0 NSCLC undergoing segmentectomy can obtain OS and LCSS similar to those obtained with lobectomy. Further studies are required considering the solid component effects and pathologic tumor types regarding segmentectomies. Additional long-term survival and outcome analyses should be conducted with larger cohorts.
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http://dx.doi.org/10.1186/s13019-022-01867-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9092858PMC
May 2022

Possible Mechanism of SARS-CoV-2 Nsp1-Mediated Control of Viral Gene Expression.

Front Cell Infect Microbiol 2022 22;12:881749. Epub 2022 Apr 22.

Guangdong Provincial Key Laboratory of Virology, Institute of Medical Microbiology, Jinan University, Guangzhou, China.

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http://dx.doi.org/10.3389/fcimb.2022.881749DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9072774PMC
April 2022
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