Publications by authors named "Yi Li"

5,767 Publications

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Sizes of crab burrows regulate water-salt transport of tidal marsh wetlands.

Mar Environ Res 2022 Jun 27;179:105691. Epub 2022 Jun 27.

School of Ecology and Nature Conservation, Beijing Forestry University, Beijing, 100083, PR China; The Key Laboratory of Ecological Protection in the Yellow River Basin of National Forestry and Grassland Administration, Beijing, 100083, PR China. Electronic address:

In recent years, scholars have paid increased attention to the ecological role of crab burrows, particularly their impact on the hydrological processes of saltmarsh wetlands. This study aims to investigate the influence of crab burrows on soil water and salt transport and to understand the ecological significance of crab burrows in coastal wetlands from the perspective of ecohydrological processes. We combined a field sample survey and an indoor soil column infiltration experiment to analyze the differences in infiltration time, soil water content, and soil electrical conductivity (EC) between different experimental groups. Consequently, the results showed that the size of crab burrow diameter varies significantly in different areas of the coastal wetland, influenced by tidal creek and sea-land distances, with larger burrow diameters in areas around 5 m from the tidal creek. Large-diameter burrows (2.5 cm × 6) are more conducive to salt transport due to their preferential water conductivity to the underlying soil vertically, small-diameter burrows (0.5 cm × 6) could promote water infiltration uniformly and maintain good soil water retention capacity. This study's results provide insights into the hydrological connectivity and spatial distribution of salinity in coastal wetlands. Additionally, the positive impact of burrows on the water-salt environment of coastal wetland sediments may also provide new ideas for coastal wetland restoration.
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http://dx.doi.org/10.1016/j.marenvres.2022.105691DOI Listing
June 2022

Porous MgCoO nanoflakes serve as electrode materials for hybrid supercapacitors with excellent performance.

J Colloid Interface Sci 2022 Jun 26;625:925-935. Epub 2022 Jun 26.

School of Materials Science and Engineering, North University of China, Taiyuan 030051, China. Electronic address:

In this work, MgCoO microspheres (MgCoO MSs) and MgCoO nanoflakes (MgCoO NFs) were prepared by one-step and two-step synthetic method, respectively, and combined with a post annealing treatment. Both MSs and NFs electrode materials possessed porous structure and large specific surface areas. The electrochemical properties were evaluated using three-electrode as well as two-electrode systems. The MgCoO NFs delivered a specific capacity of 375.5C g at 1 A g together with a high rate performance (74.9%) at 10 A g, while the MgCoO MSs exhibited 276.3C g at the current density of 1 A g. A hybrid supercapacitor (HSC) device was assembled with a cathode made from MgCoO and an anode made from activated carbon (AC) for evaluation of real applications, and it was able to run over a high voltage window (1.75 V). This MgCoO NFs//AC HSC delivered a high energy density (E, 35.4 W h kg) at 950.6 W kg, and at the highest power density (P) of 8905.0 W kg, it could still hold 25.8 W h kg. On the other hand, the MgCoO MSs//AC HSC device exhibited an E of 32.4 W h kg at a P of 1048.0 W kg. Both HSCs exhibited good long-term cycling stability due to no capacity decay over 6000 cycles at 6 A g. The excellent electrochemical performance demonstrates that these MgCoO electrode materials, especially the MgCoO NFs, have great application potential for electrochemical energy storage. This synthesis method is simple and is possibly to be applied in synthesizing other transition metal oxides (TMOs)-based electrode materials with large surface area and outstanding electrochemical performance.
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http://dx.doi.org/10.1016/j.jcis.2022.06.098DOI Listing
June 2022

The key micronutrient copper orchestrates broad-spectrum virus resistance in rice.

Sci Adv 2022 Jul 1;8(26):eabm0660. Epub 2022 Jul 1.

The State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China.

Copper is a critical regulator of plant growth and development. However, the mechanisms by which copper responds to virus invasion are unclear. We previously showed that SPL9-mediated transcriptional activation of adds a previously unidentified regulatory layer to the established ARGONAUTE (AGO18)-miR528- () antiviral defense. Here, we report that rice promotes copper accumulation in shoots by inducing copper transporter genes, including and , to counteract viral infection. Copper suppresses the transcriptional activation of by inhibiting the protein level of SPL9, thus alleviating miR528-mediated cleavage of transcripts to strengthen the antiviral response. Loss-of-function mutations in , , and caused a significant reduction in copper accumulation and plant viral resistance because of the increased SPL9-mediated transcription. Gain in viral susceptibility was mitigated when was mutated in the mutant background. Our study elucidates the molecular mechanisms and regulatory networks of copper homeostasis and the SPL9-miR528-AO antiviral pathway.
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http://dx.doi.org/10.1126/sciadv.abm0660DOI Listing
July 2022

pH-responsive dynamically cross-linked nanogels with effective endo-lysosomal escape for synergetic cancer therapy based on intracellular co-delivery of photosensitizers and proteins.

Colloids Surf B Biointerfaces 2022 Jun 16;217:112638. Epub 2022 Jun 16.

College of Materials Science and Engineering, Jilin Institute of Chemical Technology, Jilin City 132022, Jilin Province, PR China. Electronic address:

Co-delivery of photosensitizers (PSs) and protein drugs represents great potentiality for enhancing the efficiency of synergistic cancer therapy. However, the intricate tumor-microenvironment and the lack of nanoplatforms to co-deliver both into cancer cells and activate their functions significantly hinder the clinical translation of this combined approach for cancer treatment. Herein, a chlorine e6 (Ce6)-functionalized and pH-responsive dynamically cross-linked nanogel ([email protected]) is fabricated by formation of benzoic imine linkages between Ce6-modified methoxy poly (ethyleneglycol)-block-poly (diethylenetriamine)-L-glutamate-Ce6 [MPEG-b-P(Deta)LG-Ce6] and terephthalaldehyde as cross-linkers for effective intracellular co-delivery of Ce6 and cytochrome c (CC), which could form a novel combination therapy system (CC/[email protected]). The pH-sensitive benzoic imine bonds in the CC/[email protected] endow them with excellent systemic stability under normal physiological environment while this nanosystem can be further cationized to enhance cell uptake in acidic extracellular environment. Upon cellular internalization, CC/[email protected] can rapidly escape from the endo/lysosomal compartments and subsequently activate Ce6 to generate cytotoxic singlet oxygen upon laser irradiation and release of CC to induce programmed cell death by complete cleavage of benzoic imines at more acidic intracellular environments. Importantly, the catalase-like activity of CC can decompose HO to produce O for hypoxia alleviation and improvement of the photodynamic therapy (PDT) of cancer. Moreover, this enhanced synergistic anticancer activity is confirmed both in vitro and in vivo. In view of the versatile chemical conjugation, this research offers a promising and smart nanosystem for intracellular co-delivery of PSs and therapeutic proteins.
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http://dx.doi.org/10.1016/j.colsurfb.2022.112638DOI Listing
June 2022

Determination of Nucleotide Sequences within Promoter Regions Affecting Promoter Compatibility between and .

ACS Synth Biol 2022 Jun 30. Epub 2022 Jun 30.

State Key Laboratory of Biocatalysis and Enzyme Engineering, and School of Life Sciences, Hubei University, Wuhan 430062, China.

A promoter plays a crucial role in controlling the expression of the target gene in cells, thus being one of the key biological parts for synthetic biology practices. Although significant efforts have been made to identify and characterize promoters with different strengths in various microorganisms, the compatibility of promoters within different hosts still lacks investigation. In this study, we chose the native P promoter of to investigate nucleotide sequences within promoter regions affecting promoter compatibility between and . P is one of the strongest promotors in that has many excellent characteristics to be developed as microbial cell factories. Using EGFP as a reporter, a -derived P mutant library was constructed and sorted in , with candidate promoters exhibiting high fluorescence intensity collected. A total of 53 variants were finally selected and sequenced by Sanger sequencing. The sequencing results grouped these variants into 12 different P variant types, among which seven types presented higher promoter strength than native P in . The next-generation sequencing technique was then employed to identify key mutations within the P promoter region that affect the promoter compatibility. Finally, six important sites were identified and confirmed to help increase P strength in while keeping similar strength of native P in . Compared to native P, synthetic promoters combining these sites had enhanced strength; especially, P-6M combining all six sites exhibited 20-fold greater strength than native P in . This study thus not only determined six important sites affecting promoter compatibility but also confirmed a series of P promoter variants with strong promoter activity in both and . In addition, a strategy was established in this study to investigate and determine nucleotide sequences in promoter regions affecting promoter compatibility, which can be applied in other microorganisms to help reveal universal factors affecting promoter compatibility and design promoters with desired strengths among different microbial cell factories.
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http://dx.doi.org/10.1021/acssynbio.2c00187DOI Listing
June 2022

High entropy metal chalcogenides: synthesis, properties, applications and future directions.

Chem Commun (Camb) 2022 Jun 30. Epub 2022 Jun 30.

Department of Materials, The University of Manchester, Oxford Raod, M13 9PL, UK.

Metal oxides, sulphides, selenides and tellurides have routinely been investigated and utilised for a wide range of applications, in particular in the areas of energy (photovoltaic, thermoelectric) and catalysis (thermocatalysis, electrocatalysis and photoelectrocatalysis). A recent development in this chemical space is high entropy and entropy-stabilised inorganic materials, which took inspiration from preceding work on high entropy metal alloys (multicomponent alloys). High entropy inorganic materials typically have many (often ≥6) different cations or anions to yield a high configurational entropy, which can give unexpected structures and properties that are conducive to a broad range of applications in energy and catalysis. More recently still, high entropy metal chalcogenides have been developed and have been investigated for thermoelectric energy generation, batteries for energy storage and electrocatalysis. This review sets out to define entropic stabilisation in high entropy materials, along with a discussion of synthetic techniques to produce these compounds, focusing on high entropy metal chalcogenides including extant routes to high entropy sulfides, selenides and tellurides. The resulting advantageous properties of these materials due to their multi-element nature and the currently proposed and investigated applications of these materials are reviewed. We end the perspective with an opinion on future directions.
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http://dx.doi.org/10.1039/d2cc01796bDOI Listing
June 2022

Combined Channel Attention and Spatial Attention Module Network for Chinese Herbal Slices Automated Recognition.

Front Neurosci 2022 13;16:920820. Epub 2022 Jun 13.

School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou, China.

Chinese Herbal Slices (CHS) are critical components of Traditional Chinese Medicine (TCM); the accurate recognition of CHS is crucial for applying to medicine, production, and education. However, existing methods to recognize the CHS are mainly performed by experienced professionals, which may not meet vast CHS market demand due to time-consuming and the limited number of professionals. Although some automated CHS recognition approaches have been proposed, the performance still needs further improvement because they are primarily based on the traditional machine learning with hand-crafted features, resulting in relatively low accuracy. Additionally, few CHS datasets are available for research aimed at practical application. To comprehensively address these problems, we propose a combined channel attention and spatial attention module network (CCSM-Net) for efficiently recognizing CHS with 2-D images. The CCSM-Net integrates channel and spatial attentions, focusing on the most important information as well as the position of the information of CHS image. Especially, pairs of max-pooling and average pooling operations are used in the CA and SA module to aggregate the channel information of the feature map. Then, a dataset of 14,196 images with 182 categories of commonly used CHS is constructed. We evaluated our framework on the constructed dataset. Experimental results show that the proposed CCSM-Net indicates promising performance and outperforms other typical deep learning algorithms, achieving a recognition rate of 99.27%, a precision of 99.33%, a recall of 99.27%, and an F1-score of 99.26% with different numbers of CHS categories.
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http://dx.doi.org/10.3389/fnins.2022.920820DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9234258PMC
June 2022

Author Correction: A functionally conserved STORR gene fusion in Papaver species that diverged 16.8 million years ago.

Nat Commun 2022 Jun 29;13(1):3755. Epub 2022 Jun 29.

Centre for Novel Agricultural Products, Department of Biology, University of York, York, YO10 5DD, UK.

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http://dx.doi.org/10.1038/s41467-022-31568-xDOI Listing
June 2022

An Intein-Mediated Split-nCas9 System for Base Editing in Plants.

ACS Synth Biol 2022 Jun 29. Epub 2022 Jun 29.

Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, United States.

Virus-assisted delivery of the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system represents a promising approach for editing plant genomes. Among the CRISPR/Cas systems, CRISPR/Cas9 is most widely used; however, to pack the relatively large size of the CRISPR/Cas9 system into viral vectors with confined packaging capacity is challenging. To address this technical challenge, we developed a strategy based on split inteins that splits the required CRISPR/Cas9 components across a dual-vector system. The CRISPR/Cas reassembles into an active form following co-infection to achieve targeted genome editing in plant cells. An intein-mediated split system was adapted and optimized in plant cells by a successful demonstration of split-eYGFPuv expression. Using a plant-based biosensor, we demonstrated for the first time that the split-nCas9 can induce efficient base editing in plant cells. We identified several split sites for future biodesign strategies. Overall, this strategy provides new opportunities to bridge different CRISPR/Cas9 tools including base editor, prime editor, and CRISPR activation with virus-mediated gene editing.
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http://dx.doi.org/10.1021/acssynbio.1c00507DOI Listing
June 2022

Angiotensin Receptor Blockers Are Associated With a Lower Risk of Progression From Mild Cognitive Impairment to Dementia.

Hypertension 2022 Jun 29:101161HYPERTENSIONAHA12219378. Epub 2022 Jun 29.

Department of Neurology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China. (Z.D., J.J., J.W., D.P., Y.Z., H.L., X.Z.,X.L., Y.X., Y.L., Y.T.).

Background: Previous studies found that antihypertensive medications (AHMs) acting on the renin-angiotensin system had the potential to reduce the progression from mild cognitive impairment to dementia. However, it remains unclear whether this association differs between ACE (angiotensin-converting enzyme) inhibitors and angiotensin receptor blockers.

Methods: We conducted a retrospective cohort study in the Alzheimer's Disease Neuroimaging Initiative among 403 participants with hypertension and mild cognitive impairment at baseline. Information on AHMs received during the follow-up period, including angiotensin receptor blockers, ACE inhibitors, beta-blockers, calcium channel blockers, and diuretics, were self-reported. Cox proportional hazards models adjusted for potential confounders were used in the time to event analysis with progression to dementia as outcome.

Results: Of the 403 participants, the mean (SD) age was 74.0 (7.3) years, 152 (37.7%) were female, 158 (39.2%) progressed to dementia over a median follow-up time of 3.0 years. Angiotensin receptor blockers were associated with a lower risk of progression to dementia as compared to ACE inhibitors (adjusted hazard ratio=0.45 [95% CI, 0.25-0.81]; =0.023), other classes of AHMs (beta-blockers, calcium channel blockers, diuretics; adjusted hazard ratio, 0.49 [95% CI, 0.27-0.89]; =0.037), and none of AHMs (adjusted hazard ratio, 0.31 [95% CI, 0.16-0.58]; =0.001).

Conclusions: In patients with hypertension and mild cognitive impairment, angiotensin receptor blockers were associated with a lower risk of progression to dementia compared with ACE inhibitors and other classes of AHMs. Our findings may have important implications for clinical practice but still warrant further investigations in larger prospective cohorts or clinical trials.
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http://dx.doi.org/10.1161/HYPERTENSIONAHA.122.19378DOI Listing
June 2022

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Mol Pharmacol 2022 Jun 28. Epub 2022 Jun 28.

Department of Chemical Biology, School of Pharmaceutical Sciences, South-Central University for Nationalities, China

Voltage-gated K1.3 channel has been reported to be a drug target for the treatment of autoimmune diseases, and specific inhibitors of Kv1.3 are potential therapeutic drugs for multiple diseases. The scorpions could produce various bioactive peptides which could inhibit K1.3 channel. Here we identified a new scorpion toxin polypeptide gene from the venom gland cDNA library of the Chinese scorpion Sequence alignment revealed high similarities between mature peptide and previously reported K1.3 channel blockers - LmKTX10 and ImKTX88, suggesting that ImKTX58 peptide might also be a K1.3 channel blocker. By using electrophysiological recordings, we showed that recombinant ImKTX58 prepared by genetic engineering technologies had a highly selective inhibiting effect on K1.3 channel. Further alanine scanning mutagenesis and computer simulation identified four amino acid residues in ImKTX58 peptide as key binding sites to K1.3 channel by forming hydrogen bonds, salt bonds and hydrophobic interactions. Among these four residues, 28th lysine of the ImKTX58 mature peptide was found to be the most critical amino acid residue for blocking K1.3 channel. In this study, we discovered a scorpion toxin gene which has not been reported before in Hainan and successfully used prokaryotic expression system to express and purify the polypeptides encoded by this gene. Electrophysiological experiments on ImKTX58 showed that ImKTX58 has a selectively blocking effects on K1.3 channel over Kv1.1, Kv1.2, Kv1.5, SK2, SK3 and BK channels. These findings provide a theoretical basis for designing highly effective K1.3 blockers to treat autoimmune and other diseases.
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http://dx.doi.org/10.1124/molpharm.121.000480DOI Listing
June 2022

Exploring critical metabolites of honey peach (Prunus persica (L.) Batsch) from five main cultivation regions in the north of China by UPLC-Q-TOF/MS combined with chemometrics and modeling.

Food Res Int 2022 Jul 4;157:111213. Epub 2022 Apr 4.

Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing 100193, China. Electronic address:

The honey peach (Prunus persica (L.) Batsch) is the third most important worldwide fruit ranking after apples and pears. It is essential to seek the critical metabolites for identifying the origins as well as exploring potential valuable information for the honey peach. In this study, the honey peach from five main cultivation regions in the north of China were studied. Firstly, the metabolic profiling of honey peach was characterized by UPLC-Q-TOF/MS. Subsequently, the multivariate statistical techniques were performed to obtain critical metabolites. As a result, 58 metabolites were regarded as potential key markers that revealed the significant difference among the five groups. The screened critical metabolites of quercitrin, plantagoside, 3-p-coumaroylquinic acid, procyanidin, and quinic acid might have positive impacts on honey peach. Moreover, the partial least squares discriminant analysis (PLS-DA) model was developed with the critical metabolites. It gained acceptable predictive ability with the accuracy of 0.9444 for the validation set. Additionally, this study affords theoretical basis for the origin traceability of peach samples and provides the guidance for honey peach breeders for a long-range planting.
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http://dx.doi.org/10.1016/j.foodres.2022.111213DOI Listing
July 2022

Addressing Social Needs in the Clinical Setting: Description of Needs Identified in a Quality Improvement Pilot Across 3 Community Hospital Service Areas.

Popul Health Manag 2022 Jun 27. Epub 2022 Jun 27.

Enterprise Population Health, Advocate Aurora Health, Downers Grove, IL, USA.

Describe patient social needs in a large midwestern health care system. Health care systems are increasingly interested in addressing social needs in the clinical setting for improved health outcomes and lower costs. This retrospective cross-sectional analysis of health records data was a population health pilot project which tested a digital platform to screen and refer patients for social needs across three community hospitals serving Chicago and its South Suburbs. The analysis reports on social needs identified based on the referrals made. Electronic health record data were linked to the referral data to describe patient clinical and demographic characteristics. A total of 2909 patients had at least 1 social need identified. The mean age was 49 years ±21.7 and 65.7% were women. Only 33% of patients had 1 social need identified, indicating that if a social need is identified for patients, they most likely have social needs from other categories as well. Patients of color, specifically African American patients, were 14% more likely to have at least 3 social need categories identified (95% confidence interval of relative risk: 1.02-1.29). Financial stability was the most commonly reported social need (50.7%), followed by food and nutrition (43%). Transportation (22%) and housing (21%) were also commonly reported. This population health initiative reveals that many patients accessing health care have multiple social needs that can be identified at the point of care, and patterns of need-based referral vary by patient clinical and demographic characteristics.
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http://dx.doi.org/10.1089/pop.2022.0041DOI Listing
June 2022

Current status of interventional therapy for pulmonary emphysema with bronchoscopy.

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2022 May;47(5):665-672

Department of Respiratory Medicine, Branch of National Clinical Research Center for Respiratory Disease, Xiangya Hospital, Central South University, Changsha 410008.

Pulmonary emphysema is one of phenotypes of lung disease that can progress to chronic obstructive pulmonary disease (COPD). The pathogenesis is unknown, which may be closely related to smoking, infection, gene mutation, and air pollution. Pathological features of emphysema include the decreased airway elasticity at the distal end of the bronchioles and over-hyperinflation. As the incidence and mortality of COPD increase, emphysema has become the focus of research. The efficacy of drugs for COPD is limited, and currently the treatment of emphysema mainly depends on surgery. Since the concept of lung volume reduction surgery was proposed in the 1990s, many studies have confirmed improvement of lung function and exercise capacity, but the large wound, high mortality, and high cost have reduced its benefits. Lung volume reduction surgery with bronchoscopy technology, including valves, coils, sclerosants, vapor thermal ablation, etc., has the advantages of minimal invasive and decreased mortality under the basis of effectiveness. This article aims to review the current status of interventional therapy for pulmonary emphysema with bronchoscopy, compare and summarize the characteristics and applicable populations of interventional therapy, and provide theoretical support for patients' early referral and clinicians' decision-making.
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http://dx.doi.org/10.11817/j.issn.1672-7347.2022.210120DOI Listing
May 2022

Non-typhoidal salmonella invasive infections in China.

Lancet Infect Dis 2022 Jul;22(7):939

Department of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Hong Kong Special Administrative Region, China. Electronic address:

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http://dx.doi.org/10.1016/S1473-3099(22)00347-4DOI Listing
July 2022

Dehydromevalonolactone ameliorates liver fibrosis and inflammation by repressing activation of NLRP3 inflammasome.

Bioorg Chem 2022 Jun 16;127:105971. Epub 2022 Jun 16.

Key Laboratory of Biotechnology of Antibiotics, the National Health and Family Planning Commission (NHFPC), Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China. Electronic address:

Liver fibrosis is an important process in chronic liver disease and is strongly related to poor prognosis. Dehydromevalonolactone (C8) is a natural product isolated from a fungus of Fusarium sp. CPCC 401218, and its pharmacological activity has never been reported before. In this study, the potential of C8 as an anti-hepatic fibrosis agent was investigated. In human hepatic stellate cell (HSC) line LX-2, C8 suppressed the increased expression of COL1A1 and α-SMA induced by TGFβ1, which indicated that C8 could repress the activation of HSCs. In bile duct ligated rats, C8 administration (100 mg/kg, i.p.) markedly attenuated liver injury, fibrosis, and inflammation, and suppressed the expression of the macrophage surface marker F4/80. In terms of mechanism, C8 treatment blocked the activation of the NLRP3 inflammasome, which was stimulated by LPS and nigericin in bone marrow-derived macrophages (BMDMs) and companied by the release of active IL-1β. In addition, the activation of LX-2 cells induced by IL-1β released from BMDMs was also inhibited after C8 administration, which indicated that C8 repressed HSCs activation by inhibiting the activation of NLRP3 inflammasome in macrophages. Furthermore, C8 exhibited the effects of anti-fibrosis and inhibiting the expression of NLRP3 inflammasome in non-alcoholic steatohepatitis (NASH) mice. Finally, C8 can be commendably absorbed in vivo and was safe for mice at the concentration of 1000 mg/kg (p.o.). In summary, our study reveals that C8 ameliorates HSCs activation and liver fibrosis in cholestasis rats and NASH mice by inhibiting NLRP3 inflammasome in macrophages, and C8 might be a safe and effective candidate for the treatment of liver fibrosis.
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http://dx.doi.org/10.1016/j.bioorg.2022.105971DOI Listing
June 2022

Development of Photoremovable Linkers as a Novel Strategy to Improve the Pharmacokinetics of Drug Conjugates and Their Potential Application in Antibody-Drug Conjugates for Cancer Therapy.

Pharmaceuticals (Basel) 2022 May 25;15(6). Epub 2022 May 25.

Academy of Pharmacy, Xi'an Jiaotong-Liverpool University, Suzhou 215123, China.

Although there have been extensive research and progress on the discovery of anticancer drug over the years, the application of these drugs as stand-alone therapy has been limited by their off-target toxicities, poor pharmacokinetic properties, and low therapeutic index. Targeted drug delivery, especially drug conjugate, has been recognized as a technology that can bring forth a new generation of therapeutics with improved efficacy and reduced side effects for cancer treatment. The linker in a drug conjugate is of essential importance because it impacts the circulation time of the conjugate and the release of the drug for full activity at the target site. Recently, the light-triggered linker has attracted a lot of attention due to its spatiotemporal controllability and attractive prospects of improving the overall pharmacokinetics of the conjugate. In this paper, the latest developments of UV- and IR-triggered linkers and their application and potential in drug conjugate development are reviewed. Some of the most-well-researched photoresponsive structural moieties, such as UV-triggered coumarin, ortho-nitrobenzyl group (ONB), thioacetal ortho-nitrobenzaldehyde (TNB), photocaged C40-oxidized abasic site (PC4AP), and IR-triggered cyanine and BODIPY, are included for discussion. These photoremovable linkers show better physical and chemical stabilities and can undergo rapid cleavage upon irradiation. Very importantly, the drug conjugates containing these linkers exhibit reduced off-target toxicity and overall better pharmacokinetic properties. The progress on photoactive antibody-drug conjugates, such as antibody-drug conjugates (ADC) and antibody-photoabsorber conjugate (APC), as precision medicine in clinical cancer treatment is highlighted.
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http://dx.doi.org/10.3390/ph15060655DOI Listing
May 2022

Effect of Ipsilateral, Contralateral or Bilateral Repetitive Transcranial Magnetic Stimulation in Patients with Lateralized Tinnitus: A Placebo-Controlled Randomized Study.

Brain Sci 2022 Jun 2;12(6). Epub 2022 Jun 2.

Department of Ear, Nose, and Throat, Shaanxi Provincial People's Hospital, Xi'an 710068, China.

The relative benefit of ipsilateral, contralateral, and bilateral repetitive transcranial magnetic stimulation (rTMS) for tinnitus treatment remains unclear, especially for patients with lateralized tinnitus. In this study, we compared outcomes after 10 sessions of 1-Hz rTMS at 110% of resting motor threshold over a two-week period. In total, 104 right-handed patients with lateralized subjective tinnitus were randomly divided into four groups according to rTMS treatment: Left ( = 29), Right ( = 23), Bilateral ( = 30), and Sham stimulation ( = 22). Outcomes included estimates of tinnitus severity, psychological state, and psychoacoustic measures. Patients with left- or right-sided tinnitus were similarly distributed across treatment groups. There were no significant changes in outcome measures for the Right or Sham treatment groups. For the Left and Bilateral groups, tinnitus severity was significantly lower after treatment ( < 0.05). The reduction in tinnitus severity was largest for ipsilateral treatment in the Left group. The overall response rate was 56.1% for the Left group, 46.7% for the Bilateral group, 8.3% for the Right group, and 8.3% for the Sham group. For the Left and Bilateral groups, the response rate was larger for patients with left- than right-sided tinnitus. Changes in tinnitus severity were best predicted by changes in anxiety, depression, and the loudness of the tinnitus. The results suggests that rTMS on the left temporoparietal cortex is more effective for patients with left-sided than with right-sided tinnitus.
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http://dx.doi.org/10.3390/brainsci12060733DOI Listing
June 2022

Pharmacokinetics and Pharmacodynamics of Colistin Methanesulfonate in Healthy Chinese Subjects after Multi-Dose Regimen.

Antibiotics (Basel) 2022 Jun 14;11(6). Epub 2022 Jun 14.

Key Laboratory of Clinical Pharmacology of Antibiotics, National Population and Family Planning Commission, Shanghai 200040, China.

Colistin methanesulfonate (CMS) is an important treatment option for infections caused by carbapenem-resistant Gram-negative organisms (CROs). This study evaluated the pharmacokinetic/pharmacodynamic (PK/PD) profiles and safety of CMS in Chinese subjects following a recommended dosage. A total of 12 healthy Chinese subjects received CMS injections at 2.5 mg/kg once every 12 h for 7 consecutive days. The PK/PD profiles of the active form of CMS, colistin, against CROs were analyzed with the Monte Carlo simulation method. No serious adverse events were observed. The average steady-state plasma concentrations of CMS and colistin were 4.41 ± 0.75 μg/mL and 1.27 ± 0.27 μg/mL, and the steady-state exposures (AUC) were 52.93 ± 9.05 h·μg/mL and 15.28 ± 3.29 h·μg/mL, respectively. Colistin, at its minimum inhibitory concentration (MIC) of 0.5 μg/mL, has >90% probability to reduce CROs by ≥1 log. The PK/PD breakpoints for the ≥1 log kill were ≥MIC for carbapenem-resistant and , but were ≤MIC for carbapenem-resistant . The recommended dose regimen of CMS for 7 consecutive days was safe in Chinese subjects. The systemic exposure of colistin showed a high probability of being sufficient for most CROs, but was not sufficient for some carbapenem-resistant .
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http://dx.doi.org/10.3390/antibiotics11060798DOI Listing
June 2022

Biolimus-Coated Balloon in Small-Vessel Coronary Artery Disease: The BIO-RISE CHINA Study.

JACC Cardiovasc Interv 2022 Jun 25;15(12):1219-1226. Epub 2022 May 25.

General Hospital of Northern Theater Command, Shenyang, People's Republic of China. Electronic address:

Background: Drug-coated balloons are a safe and effective option for patients undergoing percutaneous coronary intervention, but prior randomized studies have exclusively used paclitaxel-coated devices.

Objectives: The aim of this study was to assess for the first time the safety and efficacy of a novel biolimus-coated balloon (BCB) in patients with small-vessel coronary disease.

Methods: In a prospective trial conducted at 10 centers in China, 212 patients with small-vessel native coronary disease (reference vessel diameter 2.0-2.75 mm, lesion length ≤25 mm) were randomized to receive a BCB or an uncoated balloon. The primary endpoint was in-segment late lumen loss at 9 months.

Results: In the per-protocol population, angiographic late lumen loss at 9 months was 0.16 ± 0.29 mm in the BCB group vs 0.30 ± 0.35 mm with the plain balloon (P = 0.001). Late luminal enlargement (positive remodeling) occurred in 29.7% of patients in the BCB group vs 9.8% of patients with plain balloons (P = 0.007). In the full analysis set population, after 12 months, target lesion failure rates were 6.7% in the BCB group vs 13.9% with the plain balloon (HR: 0.47; 95% CI: 0.19-1.16), and rates of the patient-oriented clinical outcome were 14.3% with the BCB vs 21.8% with the plain balloon (HR: 0.64; 95% CI: 0.33-1.24).

Conclusions: In this first-in-human study, a novel BCB showed superior efficacy to plain balloon angioplasty in patients with small-vessel coronary disease undergoing percutaneous coronary intervention. Positive vascular remodeling was more frequent, and there was a trend toward improved clinical outcomes. (A Randomized Trial of a Biolimus-Coated Balloon Versus POBA in Small Vessel Coronary Artery Disease [Brave]; NCT03769623).
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http://dx.doi.org/10.1016/j.jcin.2022.03.024DOI Listing
June 2022

Antifouling Conductive Composite Membrane with Reversible Wettability for Wastewater Treatment.

Membranes (Basel) 2022 Jun 16;12(6). Epub 2022 Jun 16.

Beijing Key Lab for Source Control Technology of Water Pollution, College of Environmental Science and Engineering, Beijing Forestry University, Beijing 100083, China.

Membrane fouling severely hinders the sustainable development of membrane separation technology. Membrane wetting property is one of the most important factors dominating the development of membrane fouling. Theoretically, a hydrophilic membrane is expected to be more resistant to fouling during filtration, while a hydrophobic membrane with low surface energy is more advantageous during membrane cleaning. However, conventional membrane materials do not possess the capability to change their wettability on demand. In this study, a stainless steel mesh-sulfosuccinate-doped polypyrrole composite membrane (SSM/PPY(AOT)) was prepared. By applying a negative or positive potential, the surface wettability of the membrane can be switched between hydrophilic and relatively hydrophobic states. Systematic characterizations and a series of filtration experiments were carried out. In the reduction state, the sulfonic acid groups of AOT were more exposed to the membrane surface, rendering the surface more hydrophilic. The fouling filtration experiments verified that the membrane is more resistant to fouling in the hydrophilic state during filtration and easier to clean in the hydrophobic state during membrane cleaning. Furthermore, Ca and Mg could complex with foulants, aggravating membrane fouling. Overall, this study demonstrates the importance of wettability switching in membrane filtration and suggests promising applications of the SSM/PPY(AOT) membrane.
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http://dx.doi.org/10.3390/membranes12060626DOI Listing
June 2022

The Ratio of CD226 and TIGIT Expression in Tfh and PD-1ICOSTfh Cells Are Potential Biomarkers for Chronic Antibody-Mediated Rejection in Kidney Transplantation.

J Immunol Res 2022 12;2022:5326083. Epub 2022 Jun 12.

Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.

Kidney transplantation is the ideal treatment for end-stage renal disease (ESRD). Chronic antibody-mediated rejection (CAMR) is the main cause of graft failure. Tfh and B cells are key immune cells that play important roles in CAMR. In this study, the populations of different Tfh cell phenotypes and B cell subsets in CAMR were investigated in a total of 36 patients. Based on Banff-2019, 15 patients were diagnosed with CAMR (CAMR group), 11 recipients were diagnosed with recurrent or de novo IgA nephropathy (IgAN group), and 10 patients displayed stable renal function (stable group). The Tfh and B cell subsets were analyzed by flow cytometry. The percentage and absolute number of PD-1ICOSTfh cells were significantly higher in CAMR ( < 0.05), as was the ratio of CD226Tfh cells to TIGITTfh cells ( < 0.05). Compared with stable recipients, CAMR patients had lower naïve B cells and higher unswitched memory B cells, which were also significantly related to renal function ( < 0.05). Using the logistic regression model, we concluded that the estimated glomerular filtration rate (eGFR), absolute number of PD-1ICOSTfh cells, and ratio of CD226Tfh cells to TIGITTfh cells were independent risk factors for CAMR. The combination of eGFR, PD-1ICOSTfh cells, and the ratio of CD226Tfh cells to TIGITTfh cells showed better diagnostic efficacy for CAMR than each single parameter. The collective findings show that monitoring different Tfh phenotypes and B cell subsets is beneficial to kidney transplant recipients and implicate the combination of eGFR, number of PD-1ICOSTfh cells, and ratio of CD226Tfh cells to TIGITTfh cells as a biomarker for diagnosing CAMR. The findings may also inform new strategies to identify and treat CAMR.
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http://dx.doi.org/10.1155/2022/5326083DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9206998PMC
June 2022

Partner and Relationship Characteristics Determining Intimate Partner Violence Among Women Living with HIV in Ruili, China.

AIDS Behav 2022 Jun 22. Epub 2022 Jun 22.

School of Public Health, University of Illinois at Chicago, Chicago, IL, USA.

Intimate partner violence (IPV) in China is a largely understudied, major health risk among women living with HIV. Using structured face-to-face interviews, this research examined partner and couple relationship characteristics associated with physical and sexual IPV among 219 HIV-positive women living with a male partner in Ruili, China. Twenty-nine women (13%) reported past-year occurrences of physical IPV, and 24 (11%) reported sexual IPV. Physical IPV was more common when the woman's partner was of Jingpo ethnicity, drank weekly, or learned of her HIV status indirectly from a third person. Reduced risk of physical IPV was associated with a woman's perceived confidant support that was available through either her partner or a minimum of 2 non-partner confidants. Sexual IPV was more often reported among women with a partner who drank frequently or was concurrently HIV-positive, or in situations where the woman was employed and the partner was not. Sexual IPV was less likely with a partner of Dai ethnicity than Han, the major ethnicity in China. Identifying determinants of IPV vulnerability among women living with HIV may help future interventions to achieve greater impact in similar settings.
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http://dx.doi.org/10.1007/s10461-022-03722-5DOI Listing
June 2022

Risk factors and clinical impact associated with infections caused by different types of carbapenem-resistant Klebsiella pneumoniae in China: A clinical study from 2014 to 2017.

J Infect 2022 Jun 18. Epub 2022 Jun 18.

Department of Clinical Laboratory Medicine, Second Affiliated Hospital of Zhejiang University, Hangzhou, China. Electronic address:

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http://dx.doi.org/10.1016/j.jinf.2022.05.045DOI Listing
June 2022

The Bioactivities of Phycocyanobilin from Spirulina.

Authors:
Yi Li

J Immunol Res 2022 11;2022:4008991. Epub 2022 Jun 11.

College of Bioscience and Biotechnology, Hunan Agricultural University, Hunan Provincial Engineering Research Center of Applied Microbial Resources Development for Livestock and Poultry, Changsha, Hunan 410125, China.

Phycocyanobilin (PCB) is a linear open-chain tetrapyrrole chromophore that captures and senses light and a variety of biological activities, such as anti-oxidation, anti-cancer, and anti-inflammatory. In this paper, the biological activities of PCB are reviewed, and the related mechanism of PCB and its latest application in disease treatment are introduced. PCB can resist oxidation by scavenging free radicals, inhibiting the activity of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, and delaying the activity of antioxidant enzymes. In addition, PCB can also be used as an excellent anti-inflammatory agent to reduce the proinflammatory factors IL-6 and IFN- and to up-regulate the production of anti-inflammatory cytokine IL-10 by inhibiting the inflammatory signal pathways NF-B and mitogen-activated protein kinase (MAPK). Due to the above biological activities of phycocyanobilin PCB, it is expected to become a new effective drug for treating various diseases, such as COVID-19 complications, atherosclerosis, multiple sclerosis (MS), and ischaemic stroke (IS).
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http://dx.doi.org/10.1155/2022/4008991DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9206584PMC
June 2022

G9a promotes inflammation in induced pneumonia mice by stimulating M1 macrophage polarization and H3K9me2 methylation in FOXP1 promoter region.

Ann Transl Med 2022 May;10(10):583

Graduate School, Tianjin Medical University, Tianjin, China.

Background: has become a leading cause of pneumonia in recent years. Here, we investigated the mechanism of histone methylase G9a in -induced pneumonia (Spn).

Methods: G9a expression in Spn mouse tissue was measured. G9a lentivirus interference vector was injected into Spn mice to evaluate the wet and dry weight of the right upper lobe and the total lung water content (TLW) and wet/dry ratio (W/D). The number of neutrophils, macrophages, and lymphocytes in bronchoalveolar lavage fluid (BALF) was detected, and the levels of interleukin-1β (IL-1β), IL-6, tumor necrosis factor-α (TNF-α), and IL-10 in BALF were assessed. The expressions of M1 and M2 macrophage markers were also detected. The enrichment of histone 3 lysine 9 dimethylation (H3K9me2) in the Forkhead Box P1 (FOXP1) promoter was detected by chromatin immunoprecipitation (ChIP) assay, and the transcription level of FOXP1 was detected. Mouse macrophage RAW264.7 was induced by lipopolysaccharide (LPS) following G9a interference.

Results: G9a in the lung tissue of Spn mice was increased. After G9a knockdown, the mouse weight increased, the infiltration of inflammatory cells was decreased, levels of pro-inflammatory cytokines in BALF were decreased, CD86 and inducible nitric oxide synthase (iNOS) were decreased, and CD206 and arginase-1 (Arg-1) were elevated. In LPS-induced RAW264.7, G9a inhibited macrophage polarization to M1 and promoted macrophage polarization to M2. G9a promoted H3K9me2 methylation in the FOXP1 promoter region and inhibited its transcription, while FOXP1 downregulation reversed the inhibition of G9a knockdown on macrophage polarization to M1 and the inflammatory effect on Spn mice.

Conclusions: G9a promotes M1 polarization of macrophages by promoting H3K9me2 methylation in the FOXP1 promoter region, promoting an inflammatory response in Spn mice.
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http://dx.doi.org/10.21037/atm-22-1884DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201180PMC
May 2022

The Association Between FT3 With the Outcome and Inflammation/Coagulopathy/Fibrinolysis of COVID-19.

Front Endocrinol (Lausanne) 2022 3;13:877010. Epub 2022 Jun 3.

Critical Care Medicine, The Second Xiangya Hospital, Central South University, Changsha, China.

Background: The coronavirus disease 2019 (COVID-19) pandemic has caused substantial threats to people's physical health and lives, claiming the lives of over 5 million people worldwide. It is imperative to identify the disease severity and intervene with effective therapy as early as possible. Previous studies have shown that low free triiodothyronine (FT3) may possess the predictive value on COVID-19 prognosis.

Methods: In this retrospective cohort study, 15-day clinical and laboratory data of 186 hospitalized patients of COVID-19 after admission were analyzed. Groups were based on the disease severity of COVID-19, survival or non-survival, and presence or absence of euthyroid sick syndrome (ESS). Categorical variables were compared with the chi-square test or Fisher's exact test. Continuous variables were tested by Wilcoxon rank-sum test for the non-normal distribution. Spearman correlations were used to assess the correlations between FT3 with clinic parameters of multiple time points.

Results: The non-survival patients had significant lower levels of FT3 (3.24 ± 0.42 vs. 4.19 ± 0.08 pmol/L, < 0.05) and thyroid-stimulating hormone (TSH) (0.69 ± 0.19 vs. 2.32 ± 0.2 uIU/ml, < 0.05), and the FT3 of severe patients was significantly lower than that of non-severe patients (3.67 ± 0.14 vs. 4.33 ± 0.09 pmol/L, < 0.05). Fifty-nine cases of COVID-19 patients were diagnosed with ESS. Compared with non-ESS patients, those with ESS were older and had higher proportions of fever, shortness of breath, hypertension, diabetes, severe disease, and mortality. In addition, the correlation analysis between FT3 and clinical parameters showed that FT3 were positively related to the lymphocyte count and albumin and negatively correlated with C-reactive protein, erythrocyte sedimentation rate, and D-dimer at all time points in the first 15 days after admission.

Conclusion: Low FT3 had a significant predictive value on the prognosis of COVID-19 patients, and FT3 was significantly related with clinic parameters of inflammation/coagulopathy/fibrinolysis.
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http://dx.doi.org/10.3389/fendo.2022.877010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204000PMC
June 2022

Beneficial Effect of Edoxaban on Preventing Atrial Fibrillation and Coagulation by Reducing Inflammation HBG1/HBD Biomarkers.

Front Pharmacol 2022 3;13:904317. Epub 2022 Jun 3.

Department of Cardiology, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, China.

Atrial fibrillation (AF) is the most common cardiac arrhythmia. The effectiveness and mechanism of edoxaban in preventing stroke after atrial fibrillation remain unclear. The expressions of HBG1 and HBD in red blood cells were tested in AF. Sixty C57B/6J mice were randomly divided into the following groups: the control (CON) group, atrial fibrillation (AF) group, AF + edoxaban group, and AF + rivaroxaban group. H&E staining assay and reticular fiber staining were performed. Myocardial fibrosis was evaluated by the Masson staining assay, Sirius red staining assay, and immunohistochemical assay for the expressions of α-SMA and COL1A1. ELISA and RT-PCR assay were performed for the detection of inflammatory parameters (TNF-α, IL-1β, IL-6, and IL-10). Blood lipids were detected by using the Beckman automatic biochemical analyzer. Furthermore, four items of coagulation were detected, and molecular docking among HBG1, HBD, and MASP1 (Xa) was performed by PyMOL 2.1 software. The BP neural network model, cubic spline interpolation, and support vector machine model were constructed to predict prothrombin time based on HBG1 and HBD expressions. COIP assay was performed to construct the interaction between HBG1 and HBD. The functional enrichment analysis was performed by DAVID and Metascape tools. : The expressions of HBG1 and HBD in red blood cells of the patients with atrial fibrillation were decreased. The results showed a lower level of hemoglobin in red blood cells with HBG1-siRNA and HBG1-siRNA. Compared with the AF group, the collagen fiber percentage in the AF + edoxaban group was decreased ( < 0.05). After using edoxaban, the expressions of TNF-α, IL-1β, IL-6, and IL-10 were significantly decreased ( < 0.05). The LDL-C, TC, and TG levels were downregulated in the AF + edoxaban group. The PT and APTT levels in the AF + edoxaban group were more increasing than in the AF mice ( < 0.05). Compared with the AF group, the expressions of HBG1 and HBD were downregulated in the AF + edoxaban group ( < 0.05). HBG1 protein matched well with HBD and MASP1(Xa) protein surfaces. There exists a significant interaction between HBG1, HBD, and PT the BP neural network and support vector machine. Enrichment analysis showed that HBG1 and HBD were mainly enriched in blood coagulation. Edoxaban could prevent atrial fibrillation and coagulation by reducing inflammation, lipids, and fibrosis HBG1/HBD biomarkers effectively, and the effect was superior to that of rivaroxaban.
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http://dx.doi.org/10.3389/fphar.2022.904317DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204214PMC
June 2022

Factors Associated With Newly HIV Infection and Transmitted Drug Resistance Among Men Who Have Sex With Men in Harbin, P.R. China.

Front Public Health 2022 2;10:860171. Epub 2022 Jun 2.

Chinese Center for Disease Control and Prevention, Beijing, China.

Background: This study aimed to evaluate HIV incidence, factors associated with HIV incidence and transmitted drug resistance (TDR) among newly infected men who have sex with men (MSM) in Harbin, P.R. China.

Methods: A cohort study was conducted among MSM in Harbin during 2013 and 2018, with a follow-up frequency of every 6 months. Blood samples from MSM were tested for HIV antibodies, RNA was extracted from plasma, and the gene was sequenced, and genotypic drug-resistance analyses were performed.

Results: From 2013 to 2018, the overall rate of HIV incidence was 3.55/100 PY. Syphilis infection, unprotected sex with men in the past 6 months, and unawareness of HIV/AIDS knowledge were risk factors for HIV seroconversion. The distribution of HIV genotypes was as follows: CRF01_AE, 57.1%; CRF07_BC, 28.5%; CRF55_01B, 2.0%; B, 8.2%. The prevalence of transmitted drug resistance was 4.08%.

Conclusion: HIV incidence in MSM in Harbin is moderately high, and transmitted drug resistance exists in the population.
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http://dx.doi.org/10.3389/fpubh.2022.860171DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201057PMC
June 2022

Impact of high-density EEG in presurgical evaluation for refractory epilepsy patients.

Clin Neurol Neurosurg 2022 Jun 11;219:107336. Epub 2022 Jun 11.

Stanford University Department of Neurology and Neurological Sciences, Palo Alto, CA 94304, USA.

Objective: Electrical source localization (ESI) can help to identify the seizure onset zone or propagation zone, but it is unclear how dipole localization techniques influence surgical planning.

Methods: Patients who received a high density (HD)-EEG from 7/2014-7/2019 at Stanford were included if they met the following inclusion criteria: (1) adequate epileptiform discharges were recorded for source localization analysis, (2) underwent surgical treatment, which was at least 6 months before the survey. Interictal ESI was performed with the LORETA method on age matched MRIs. Six neurophysiologists from the Stanford Epilepsy Program independently reviewed each case through an HIPPA-protected online survey. The same cases were presented again with additional data from the HD-EEG study. Ratings of how much the HD-EEG findings added value and in what way were recorded.

Results: Fifty out of 202 patients met the inclusion criteria, providing a total of 276 h of HDEEG recordings. All patients had video EEG recordings and at least one brain MRI, 88 % had neuropsychological testing, 78 % had either a PET or SPECT scan. Additional HD-EEG information was rated as helpful in 83.8 %, not useful in 14.4 % and misleading in 1.8 % of cases. In 20.4 % of cases the HD-EEG information altered decision-making in a major way, such as choosing a different surgical procedure, avoidance of invasive recording or suggesting placement of invasive electrodes in a lobe not previously planned. In 21.5 % of cases, HD-EEG changed the plan in a minor way, e.g., extra invasive electrodes near the previously planned sites in the same sub-lobar region. In 42.3 % cases, HD-EEG did not change their plan but provided confirmation. In cases with normal MRI, additional HD-EEG information was more likely to change physicians' decision making during presurgical process when compared to the cases with MRI-visible lesions (53.3 % vs. 34.3 %, p = 0.002). Among patients achieving Engel class I/II outcome, the concordance rate of HD-EEG and resection zone was 64.7 % versus 35.3 % with class III/IV (p = 0.028).

Conclusion: HD-EEG assists presurgical planning for refractory epilepsy patients, with a higher yield in patients with non-lesional MRIs. Concordance of HD-EEG dipole analysis localization and resection site is a favorable outcome indicator.
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http://dx.doi.org/10.1016/j.clineuro.2022.107336DOI Listing
June 2022
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