Publications by authors named "Yi Juan Teo"

3 Publications

  • Page 1 of 1

A 3D pancreatic tumor model to study T cell infiltration.

Biomater Sci 2021 Nov 9;9(22):7420-7431. Epub 2021 Nov 9.

Singapore Immunology Network, A*STAR, 8A Biomedical Groove, 138648, Singapore.

The desmoplastic nature of the pancreatic ductal adenocarcinoma (PDAC) tumor microenvironment (TME) prevents the infiltration of T cells and the penetration of chemotherapeutic drugs, posing a challenge to the validation of targeted therapies, including T cell immunotherapies. We present an 3D PDAC-TME model to observe and quantify T cell infiltration across the vasculature. In a three-channel microfluidic device, PDAC cells are cultured in a collagen matrix in the central channel surrounded, on one side, by endothelial cells (ECs) to mimic a blood vessel and, on the opposite side, by pancreatic stellate cells (PSCs) to simulate exocrine pancreas. The migration of T cells toward the tumor is quantified based on their activation state and TME composition. The presence of EC-lining drastically reduces T cell infiltration, confirming the essential role of the vasculature in controlling T cell trafficking. We show that activated T cells migrate ∼50% more than the not-activated ones toward the cancer cells. Correspondingly, in the absence of cancer cells, both activated and not-activated T cells present similar migration toward the PSCs. The proposed approach could help researchers in testing and optimizing immunotherapies for pancreatic cancer.
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http://dx.doi.org/10.1039/d1bm00210dDOI Listing
November 2021

Renal CD169 resident macrophages are crucial for protection against acute systemic candidiasis.

Life Sci Alliance 2021 05 19;4(5). Epub 2021 Feb 19.

School of Biological Sciences, Nanyang Technological University, Singapore, Singapore

Disseminated candidiasis remains as the most common hospital-acquired bloodstream fungal infection with up to 40% mortality rate despite the advancement of medical and hygienic practices. While it is well established that this infection heavily relies on the innate immune response for host survival, much less is known for the protective role elicited by the tissue-resident macrophage (TRM) subsets in the kidney, the prime organ for persistence. Here, we describe a unique CD169 TRM subset that controls growth and inflammation during acute systemic candidiasis. Their absence causes severe fungal-mediated renal pathology. CD169 TRMs, without being actively involved in direct fungal clearance, increase host resistance by promoting IFN-γ release and neutrophil ROS activity.
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http://dx.doi.org/10.26508/lsa.202000890DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7918719PMC
May 2021

Type 1 Conventional CD103 Dendritic Cells Control Effector CD8 T Cell Migration, Survival, and Memory Responses During Influenza Infection.

Front Immunol 2018 21;9:3043. Epub 2018 Dec 21.

School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.

Type 1 conventional CD103 dendritic cells (cDC1) contribute significantly to the cytotoxic T lymphocyte (CTL) response during influenza virus infection; however, the mechanisms by which cDC1s promote CTL recruitment and viral clearance are unclear. We demonstrate that cDC1 ablation leads to a deficient influenza-specific primary CD8 T cell response alongside severe pulmonary inflammation, intensifying susceptibility to infection. The diminished pulmonary CTL population is not only a consequence of reduced priming in the lymph node (LN), but also of dysregulated CD8 T cell egression from the LN and reduced CD8 T cell viability in the lungs. cDC1s promote S1PR expression on CTLs, a key chemokine receptor facilitating CTL LN egress, and express high levels of the T cell survival cytokine, IL-15, to support CTL viability at the site of infection. Moreover, cDC1 ablation leads to severe impairment of CD8 T cell memory recall and cross-reactive protection, suggesting that cDC1 are not only involved in primary T cell activation, but also in supporting the development of effective memory CD8 T cell precursors. Our findings demonstrate a previously unappreciated and multifaceted role of CD103 DCs in controlling pulmonary T cell-mediated immune responses.
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http://dx.doi.org/10.3389/fimmu.2018.03043DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6308161PMC
October 2019
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