Publications by authors named "Yi Ji"

381 Publications

[Corrigendum] HIF-1α contributes to tube malformation of human lymphatic endothelial cells by upregulating VEGFR-3.

Int J Oncol 2022 Oct 3;61(4). Epub 2022 Aug 3.

Department of Burns and Plastic Surgery, Children's Hospital of Nanjing Medical University, Nanjing, Jiangsu 210008, P.R. China.

Subsequently to the publication of the above article, the authors have realized that Fig. 4 on p. 145 (showing the results from the colony formation assay) was published containing erroneous images in Fig. 4C. Essentially, incorrect images were selected to represent the negative control (NC) experiments owing to an altered shooting angle and incorrect naming of the files. The corrected version of Fig. 4, now showing the correct data for the NC experiments in Fig. 4C, is shown on the next page. The authors confirm that the error made in the presentation of Fig. 4 did not adversely affect either the results or the conclusions reported in this paper, and they are grateful to the Editor of for granting them this opportunity to publish a Corrigendum. They also apologise to the readership for any inconvenience caused. [International Journal of Oncology 54: 139-151, 2019; DOI: 10.3892/ijo.2018.4623].
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http://dx.doi.org/10.3892/ijo.2022.5403DOI Listing
October 2022

Development and Application of a Visual Duck Meat Detection Strategy for Molecular Diagnosis of Duck-Derived Components.

Foods 2022 Jun 26;11(13). Epub 2022 Jun 26.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.

To make meat adulteration detection systems faster, simpler and more efficient, we established a duck-derived meat rapid detection Recombinase Polymerase Amplification (dRPA) method by using interleukin 2 (IL-2) from nuclear genomic DNA as the target gene to design specific primers. We tested the dRPA detection system by comparing its sensitivity and specificity using real-time fluorescent PCR technology. By adjusting the ratio of reagents, this method shortens the time of DNA extraction and visualizes results in combination with colloidal gold immunoassay strips. Our results demonstrate that this dRPA method could specifically detect duck-derived components with a sensitivity of up to 23 copies/μL and the accuracy of the results is consistent with real-time fluorescent PCR. Additionally, dRPA can detect at least 1% of the duck meat content by mixing beef and mutton with duck meat in different proportions, which was verified by spot-check market samples. These results can be visualized with colloidal gold immunoassay strips with the same accuracy as real-time fluorescent RPA. dRPA can complete detection within 30 min, which shortens existing detection time and quickly visualizes the detection results on-site. This lays the groundwork for future large-scale standardized duck origin detection.
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http://dx.doi.org/10.3390/foods11131895DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9266182PMC
June 2022

Non-interferometric volumetric imaging in living human retina by confocal oblique scanning laser ophthalmoscopy.

Authors:
Wenjun Shao Ji Yi

Biomed Opt Express 2022 Jun 24;13(6):3576-3592. Epub 2022 May 24.

Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland, 21231, USA.

Three-dimensional (3D) imaging of the human retina is instrumental in vision science and ophthalmology. While interferometric retinal imaging is well established by optical coherence tomography (OCT), non-interferometric volumetric imaging in the human retina has been challenging up to date. Here, we report confocal oblique scanning laser ophthalmoscopy (CoSLO) to fill that void and harness non-interferometric optical contrast in 3D. CoSLO decouples the illumination and detection by utilizing oblique laser scanning and oblique imaging to achieve ∼4x better axial resolution than conventional SLO. By combining remote focusing, CoSLO permits the acquisition of depth signals in parallel and over a large field of view. Confocal gating is introduced by a linear sensor array to improve the contrast and resolution. For the first time, we reported non-interferometric 3D human retinal imaging with >20° viewing angle, and revealed detailed features in the inner, outer retina, and choroid. CoSLO shows potential to be another useful technique by offering 3D non-interferometric contrasts.
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http://dx.doi.org/10.1364/BOE.457408DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208584PMC
June 2022

Maternal and Perinatal Risk Factors for Infantile Hemangioma: A Matched Case-Control Study with a Large Sample Size.

Dermatol Ther (Heidelb) 2022 Jul 25;12(7):1659-1670. Epub 2022 Jun 25.

Division of Oncology, Department of Pediatric Surgery, West China Hospital of Sichuan University, 37 Guo-Xue-Xiang, Chengdu, 610041, China.

Introduction: Infantile hemangioma (IH) is the most common benign tumor in infancy, but information about its pathogenesis is limited. The aim of this study was to determine maternal and perinatal risk factors for IH.

Methods: A total of 1033 IH patients were enrolled in the study between 2017 and 2020. IH patients were matched with controls by sex. Trained investigators collected detailed information from the participants. Logistic regression models were used for multivariate analysis.

Results: The statistical analysis demonstrated that miscarriage history (odds ratio [OR] = 4.275; 95% confidence interval [CI] [3.195, 5.720]), anemia in pregnancy (OR = 4.228; 95% CI [3.083, 5.799]), preterm premature rupture of membranes (PPROM) (OR = 3.182; 95% CI [1.359, 7.454]), placenta previa (OR = 2.440; 95% CI [1.787, 3.333]), threatened miscarriage (OR = 2.290; 95% CI [1.726, 3.039]), premature rupture of membranes (PROM) (OR = 1.785; P < 0.05), progesterone use (OR = 1.614; P < 0.001) and abnormal amniotic fluid volume (OR = 1.499; P < 0.05) were independent risk factors for IH. Gestational diabetes mellitus (GDM) (OR = 0.607; 95% CI [0.464, 0.794]), multiple gestations (OR = 0.407; 95% CI [0.232, 0.713]), hypothyroidism (OR = 0.407; 95% CI [0.227, 0.730]) and uterine fibroids (OR = 0.393; 95% CI [0.250, 0.618]) may reduce the risk of IH.

Conclusions: Maternal and perinatal factors are closely associated with IH occurrence. Our study provides reliable clues to guide further exploration of the pathogenesis of IH.

Trial Registration: ClinicalTrials.gov, NCT03331744. Infantile hemangioma is the most common benign tumor in children, which seriously affects appearance and function and even threatens life. The pathogenesis is not clear, a detailed case-control study of the maternal and perinatal periods with a large sample size will facilitate the development of individualized and precise treatment, early and timely interventions for high-risk children and improvement of prognosis. Our study found that miscarriage history, anemia in pregnancy, preterm premature rupture of membranes (PPROM), placenta previa, threatened miscarriage, premature rupture of membranes (PROM), progesterone use and abnormal amniotic fluid volume were independent risk factors for IH.
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http://dx.doi.org/10.1007/s13555-022-00756-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9276869PMC
July 2022

Infantile hepatic hemangiomas: looking backwards and forwards.

Precis Clin Med 2022 Mar 11;5(1):pbac006. Epub 2022 Feb 11.

Division of Oncology, Department of Pediatric Surgery, West China Hospital of Sichuan University, Chengdu 610041, China.

Infantile hepatic hemangiomas (IHHs) are common benign tumors seen in the liver of infants. IHHs are true infantile hemangiomas (IHs) and have phases of proliferation and involution parallel to those of cutaneous IHs. The definition and classification of IHH are still confusing in the literature. The mechanisms during the pathogenesis of IHH have yet to be discovered. The clinical manifestations of IHH are heterogeneous. Although most IHH lesions are asymptomatic, some lesions can lead to severe complications, such as hypothyroidism, consumptive coagulopathy, and high-output congestive cardiac failure. Consequently, some patients can possibly encounter a fatal clinical condition. The heterogeneity of the lesions and the occurrence of disease-related comorbidities can make the treatment of IHH challenging. Oral propranolol is emerging as an effective systemic approach to IHH with obvious responses in tumor remission and symptom regression. However, the precise clinical characteristics and treatment strategies for patients with severe IHH have not yet been well established. Here, we summarize the epidemiology, pathogenic mechanism, clinical manifestations, diagnosis, and treatment of IHH. Recent updates and future perspectives for IHH will also be elaborated.
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http://dx.doi.org/10.1093/pcmedi/pbac006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8982613PMC
March 2022

Treated Pierre Robin Sequence Using Placed Allogenic Acellular Bone Matrix and Mandibular Distraction Osteogenesis in the Neonate.

Front Pediatr 2022 23;10:890156. Epub 2022 May 23.

Department of Burns and Plastic Surgery, Children's Hospital of Nanjing Medical University, Nanjing, China.

Objective: The aim of the study was to report our experience with placed allogenic acellular bone matrix and mandibular distraction osteogenesis in Pierre Robin sequence (PRS), and explore the role of distraction in the osteogenesis of acellular bone.

Materials And Methods: A total of 428 neonates with severe PRS managed with placing allogenic acellular bone and bilateral mandibular distraction osteogenesis were included in the study. The procedure included using oblique-shaped osteotomy, fixing bilateral mandibular distractor, instantly extending a 4-6 mm gap, and placing allogenic acellular bone into the gap. The length of allogenic acellular bone was 4-5 mm. Although the surgical techniques, distraction, and consolidation periods were similar, the allogenic acellular bone matrix we placed was quite different from the traditional distraction. With the technology we used, tracheal intubation could be immediately removed, thus quickly improving breathing conditions compared to traditional methods after the surgery. The jaw extending and oral feeding could begin on the 5th day. The jaw was extended 0.6 mm twice a day until the mandible was overcorrected by 20%.

Results: All 428 cases included in this study were successfully extubated after the operation, and the difficulty in breathing was instantly relieved. Total mandibular distraction was 15-20 mm. Oral feeding was started at 6 h to 6 days postoperatively, while hospital stay ranged from 18 to 20 days postoperatively. No major complications were reported. Medium to long-term results was good. Mandibular distractors were removed after 3 months.

Conclusions: Bilateral mandibular distraction osteogenesis combined with placing allogenic acellular bone in the neonate are safe and accurate procedures, which are the primary treatment options for cases of severe PRS. It can be considered that the tension of distraction can promote osteogenesis in acellular bone and thus improve distractive effect of the mandible.
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http://dx.doi.org/10.3389/fped.2022.890156DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168749PMC
May 2022

Transumbilical single-site two incision laparoscopic pyloromyotomy for pediatric hypertrophic pyloric stenosis.

BMC Surg 2022 Jun 7;22(1):218. Epub 2022 Jun 7.

Department of Pediatric Surgery, West China Hospital of Sichuan University. #37 Guo-Xue-Xiang, Chengdu, 610041, Sichuan, China.

Purpose: A new novel technique for the treatment of pediatric hypertrophic pyloric stenosis (HPS), transumbilical single-site laparoscopic pyloromyotomy with a single instrument (TUSSLP), was introduced. TUSSLP was compared with the transabdominal three-site laparoscopic pyloromyotomy (TATSLP) procedure.

Methods: Patients with HPS who underwent TUSSLP and TATSLP between January 2016 and September 2020 were assigned to group A and group B, respectively. The descriptive variables, perioperative clinical characteristics and postoperative follow-up results were retrospectively analyzed and compared between the 2 groups. The primary outcome of this study was the rate of switching to conventional pyloromyotomy.

Results: Sixty-four patients were enrolled in this study. Of these patients, 29 (22 males, 7 females, 54.4 ± 22.6 days) who received TUSSLP were assigned to group A. The remaining 35 (28 males, 7 females, 54.5 ± 27.6 days) who received TATSLP were assigned to group B. The data of preoperative patient variables were comparable between the 2 groups (P > 0.05). The mean operative time (ORT) was 28.1 ± 5.6 min in group A, which was not significantly different from 25.8 ± 3.1 min in group B (P = 0.25). The other perioperative features were not significantly different between the 2 groups (P > 0.05). During follow-up (39.1 ± 14.7 m in group A and 35.4 ± 16.1 m in group B, P = 0.51), no significant difference was observed in the overall incidence of vomiting between the 2 groups (P = 0.26).

Conclusions: TUSSLP is a feasible and reliable minimally invasive method for HPS. It has the advantages of an improved cosmetic appearance. The postoperative follow-up results of TUSSLP are comparable with those of TATSLP.
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http://dx.doi.org/10.1186/s12893-022-01672-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9172057PMC
June 2022

A Baseline Study of Oxygen Saturation in Parafoveal Vessels Using Visible Light Optical Coherence Tomography.

Front Med (Lausanne) 2022 12;9:886576. Epub 2022 May 12.

Department of Ophthalmology, School of Medicine, Johns Hopkins University, Baltimore, MD, United States.

The retinal macula is at the center of our visual field, and thus pathological damage in the macula significantly impacts an individual's quality of life. The parafoveal vessels form the inner retina provide oxygen perfusion, and the measurement of parafoveal oxygen saturation (sO) can evaluate macular metabolism and provide pathophysiological insight. In this paper, for the first time, we present a baseline study of microvascular oxygen saturation (sO) in perifoveal macular region using visible light optical coherence tomography (VIS-OCT) on normal eyes. The arterial and venous sO from all eyes was 92.1 ± 7.1 (vol %) and 48.4 ± 5.0 (vol %) (mean ± SD), respectively. Arteriovenous sO difference was 43.8 ± 9.5 (vol %). Marginal correlation was found between venous sO and intraocular pressure (IOP) among eyes. No significant correlation was found between sO and vessel topological features, including length, diameter, and distance to fovea. This baseline study could serve as a benchmark for the future sO investigation of retinal macular pathologies.
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http://dx.doi.org/10.3389/fmed.2022.886576DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9133487PMC
May 2022

Amylose Inter-Chain Entanglement and Inter-Chain Overlap Impact Rice Quality.

Foods 2022 May 23;11(10). Epub 2022 May 23.

Jiangsu Key Laboratory of Crop Genetics and Physiology, State Key Laboratory of Hybrid Rice, College of Agriculture, Yangzhou University, Yangzhou 225009, China.

Retrogradation of cooked rice happens in two ways: one is by the formation of ordered structures, and the other is through intra- and inter-chain entanglement and inter-chain overlap, which in turn are affected by the amylose chain-length distribution. Both entanglement and overlap could affect rice texture. Here, four amylose samples were isolated from starch by precipitation from a dimethyl sulfoxide solution with butan-1-ol and isoamyl alcohol. Following enzymatic debranching, they were then characterized using size-exclusion chromatography. Amylose solutions (10%, /) were made by dissolving amylose in 90% (/) DMSO. Amylose gels (10%, /) were made by dissolving amylose powders into hot water, followed by cooling. The rigidity of the amylose gels and the structural order were measured using a texture analyzer and X-ray diffractometer, respectively. In the amylose solution, for a given mass of polymer in a fixed amount of solvent, the total occupied volume was reduced when the polymer molecular weight was smaller, resulting in less inter-chain overlap and a lower viscosity of the amylose solution. The overall mobility and diffusion of the molecules were inversely related to the square of the molecular weight until the gelation concentration. Thus, amylose gels in which amylose had a lower molecular weight had a greater chance to permeate into other molecules, which counterintuitively led to more inter-chain entanglement and more rigid amylose gels during retrogradation. This information could help rice breeders improve rice quality by using the molecular structure of starch as a guide.
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http://dx.doi.org/10.3390/foods11101516DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9141181PMC
May 2022

Impact of local living environment on innovation efficiency of high-tech industries in China: a spatial analysis.

Authors:
Yi Ji Zilong Wang

Environ Sci Pollut Res Int 2022 May 27. Epub 2022 May 27.

College of Economics and Management, Nanjing University of Aeronautics and Astronautics, No.29 Jiangjun Road, Jiangning District, Nanjing, 211106, People's Republic of China.

Environmental problems not only relate to residents' happiness but also challenge the innovation development of industries. This study first measures the innovation efficiency of China's high-tech industries using the super-efficiency data envelopment analysis model and portrays its spatial characteristics through the Moran's I index and the local indicators of spatial association map. Second, we use the entropy weight method to construct the local living environment index from both natural and social environments. Finally, we utilize spatial econometric models to analyze the impact of local living environment on high-tech industries' innovation efficiency. The results reveal that, first, the spatial variation of innovation efficiency in China's high-tech industries is significant, with efficiency being higher in the east than the Midwest, and higher in the south than the north. Second, innovation efficiency has a positive adjacent and geographical spatial autocorrelation, and low-low agglomeration and low-high agglomeration dominate the types of spatial correlation. Finally, the contribution of the local living environment to the innovation efficiency of high-tech industries is positive and significant. This contribution has an obvious spatial spillover effect and regional heterogeneity. This study can help regional governments to improve local living environments and promote industrial innovation and development.
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http://dx.doi.org/10.1007/s11356-022-21038-8DOI Listing
May 2022

Application of Computer-Assisted Preoperative Planning in Treating Congenital Bilateral Zygomatico-Maxillo-Mandibular Fusion: A Case Report and Literature Review.

Cleft Palate Craniofac J 2022 Apr 19:10556656211064775. Epub 2022 Apr 19.

159388Children's Hospital of Nanjing Medical University.

Congenital fusion of the jaws (syngnathia) is a rare facial malformation with an unknown etiology. This disease may vary in severity with adhesion of soft tissue and bony fusion. It can be anterior fusion, unilateral or bilateral fusion, and complete fusion. The main problem of these patients is the difficulty of airway maintenance and feeding, and the most common postoperative complication is the relapse of bony fusion. Here, we report a young male patient with bony syngnathia, involving bilateral fusion of the ascending ramus and body of the mandible with the maxillary complex. We performed bone isolation by computer-assisted preoperative planning and used an insertional temporalis flap to fix the wound surface to prevent refusion of bone.
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http://dx.doi.org/10.1177/10556656211064775DOI Listing
April 2022

Data mining analysis reveals key acupoints and meridians for the treatment of chemotherapy-induced peripheral neuropathy.

Explore (NY) 2022 Apr 7. Epub 2022 Apr 7.

Department of Oncology, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu; Department of Oncology, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing, Jiangsu. Electronic address:

Objective: To explore effective acupoints and combinations for the treatment of chemotherapy-induced peripheral neuropathy (CIPN) METHODS: Clinical controlled trials and randomized controlled trials of acupuncture for CIPN were sourced from PubMed, Embase, Web of Science, and Chinese databases, including the Wanfang database, VIP Journals database, and China National Knowledge Infrastructure database. The quality of eligible research was evaluated based on CONSORT and STRICTA statements. The common acupoints, meridians, and acupoint combinations were determined from acupuncture prescriptions reporting positive effects and were analyzed using SPSS 23.0 and SPSS Modeler 14.1. Finally, a complex network was constructed using Cytoscape 3.8.2 to explore the core acupoints.

Results: The quality of 24 clinical trials was evaluated, and 20 acupuncture prescriptions that reported positive outcomes were included in subsequent data mining analysis. The most frequently used acupoints are ST36, LI11, LI4, LR3, and SP6. Meanwhile, they are also the core acupoints in acupuncture prescriptions according to the complex network with 28 nodes and 177 edges. The most commonly used meridians were the large intestine, stomach, and spleen. Acupoint combinations of LI11 and ST36, SP6 and ST36 were frequently used.

Conclusion: Our study provides a reference for the selection of effective acupoints for CIPN treatment and a basis for the effective use of this form of traditional Chinese medicine. Furthermore, we found limitations in the design and implementation of the available clinical research, which should be minimized in future studies.
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http://dx.doi.org/10.1016/j.explore.2022.04.003DOI Listing
April 2022

Transumbilical Single-Site Laparoscopic Intraperitoneal Closure of the Internal Inguinal Ring for Pediatric Inguinal Hernia.

Front Pediatr 2022 15;10:855537. Epub 2022 Mar 15.

Department of Pediatric Surgery, West China Hospital of Sichuan University, Chengdu, China.

Background: A new novel technique for pediatric inguinal hernia (PIH) repair, namely, transumbilical single-site laparoscopic intraperitoneal closure (TUSLIC) of the internal inguinal ring (IIR) with a single instrument, was introduced. The short-term follow-up of TUSLIC for PIH was compared with that of transabdominal multiple-site laparoscopic extraperitoneal closure (TAMLEC) for PIH.

Methods: Descriptive variables, perioperative clinical features, and short-term outcomes were retrospectively analyzed and compared between the patients who underwent TUSLIC and those who underwent TAMLEC.

Results: In total, 289 patients were enrolled in this study. Of these, 190 patients received TUSLIC, and 99 patients received TAMLEC. The descriptive variables (including sex, age, weight, and preoperative diagnosis of patients) were comparable between the two groups (-values were 0.12, 0.71, 0.69, and 0.23, respectively). The mean operative times for unilateral hernia repair and bilateral hernia repairs in TAMLEC group were significantly less than those in TUSLIC group ( < 0.01). The values of surgical site infection, umbilical bleeding, testicular atrophy, iatrogenic ascent of the testis, and secondary hydrocele were not significantly different between the two groups. There were no suture granulomas, and recurrence occurred in TUSLIC group, though at a significantly lower rate than in TAMLEC group ( < 0.05).

Conclusions: TUSLIC is a feasible, safe, and reliable minimally invasive method for PIH. Compared with TAMLEC, TUSLIC has the advantages of minimized complications and a low recurrence rate.
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http://dx.doi.org/10.3389/fped.2022.855537DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8964975PMC
March 2022

Mapping cerebral atrophic trajectory from amnestic mild cognitive impairment to Alzheimer's disease.

Cereb Cortex 2022 Apr 3. Epub 2022 Apr 3.

Department of Radiology, Tianjin Medical University General Hospital, Tianjin 300052, China.

Alzheimer's disease (AD) patients suffer progressive cerebral atrophy before dementia onset. However, the region-specific atrophic processes and the influences of age and apolipoprotein E (APOE) on atrophic trajectory are still unclear. By mapping the region-specific nonlinear atrophic trajectory of whole cerebrum from amnestic mild cognitive impairment (aMCI) to AD based on longitudinal structural magnetic resonance imaging data from Alzheimer's disease Neuroimaging Initiative (ADNI) database, we unraveled a quadratic accelerated atrophic trajectory of 68 cerebral regions from aMCI to AD, especially in the superior temporal pole, caudate, and hippocampus. Besides, interaction analyses demonstrated that APOE ε4 carriers had faster atrophic rates than noncarriers in 8 regions, including the caudate, hippocampus, insula, etc.; younger patients progressed faster than older patients in 32 regions, especially for the superior temporal pole, hippocampus, and superior temporal gyrus; and 15 regions demonstrated complex interaction among age, APOE, and disease progression, including the caudate, hippocampus, etc. (P < 0.05/68, Bonferroni correction). Finally, Cox proportional hazards regression model based on the identified region-specific biomarkers could effectively predict the time to AD conversion within 10 years. In summary, cerebral atrophic trajectory mapping could help a comprehensive understanding of AD development and offer potential biomarkers for predicting AD conversion.
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http://dx.doi.org/10.1093/cercor/bhac137DOI Listing
April 2022

"Drug-Carrier" Synergy Therapy for Amyloid-β Clearance and Inhibition of Tau Phosphorylation via Biomimetic Lipid Nanocomposite Assembly.

Adv Sci (Weinh) 2022 05 20;9(14):e2106072. Epub 2022 Mar 20.

Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education), State Key Laboratory of Natural Medicines, Department of Pharmaceutics, China Pharmaceutical University, Nanjing, 210009, China.

Amyloid-β (Aβ) toxicity is considered to be companioned by Tau phosphorylation in Alzheimer's disease (AD). The clinical AD therapy is usually subjected to low blood-brain barrier (BBB) penetration and complex interaction mechanisms between Aβ and phosphorylated Tau. A "Drug-Carrier" synergy therapy is herein designed to simultaneously target Aβ and Tau-associated pathways for AD treatment. To imitate natural nanoparticle configuration, the endogenous apolipoprotein A-I and its mimicking peptide 4F fused angiopep-2 (Ang) are sequentially grafted onto lipid nanocomposite (APLN), providing liberty of BBB crossing and microglia targeted Aβ clearance. For synergy treatment, methylene blue (MB) is further assembled into APLN (APLN/MB) for Tau aggregation inhibition. After intravenous administration, the optimized density (5 wt%) of Ang ligands dramatically enhances APLN/MB intracerebral shuttling and accumulation, which is 2.15-fold higher than that Ang absent-modification. The site-specific release of MB collaborates APLN to promote Aβ capture for microglia endocytosis clearance and reduce p-Tau level by 25.31% in AD pathogenesis. In AD-Aβ-Tau bearing mouse models, APLN/MB can relieve AD symptoms, rescue neuron viability and cognitive functions. Collectively, it is confirmed that "Drug-Carrier" synergy therapy of APLN/MB is a promising approach in the development of AD treatments.
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http://dx.doi.org/10.1002/advs.202106072DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9108666PMC
May 2022

Health-Related Quality of Life in Children With Kaposiform Hemangioendothelioma.

Front Pediatr 2022 24;10:720611. Epub 2022 Feb 24.

Division of Oncology, Department of Pediatric Surgery, West China Hospital of Sichuan University, Chengdu, China.

Background And Objective: Kaposiform hemangioendothelioma (KHE) is a rare, aggressive and borderline vascular tumor mainly occurring in infants and children. The aim of this study was to determine the health-related quality of life (HRQOL) in children with KHE.

Measures: A total of 91 children with KHE participated in this cross-sectional study. The HRQOL was assessed by the age-specific Pediatric Quality of Life Inventory Version 4.0 (PedsQL™ 4.0) Infant Scales, Family Information Form (FIF), Family Impact Module (FIM) and Generic Core Scales (GCS). For comparison, demographically matched healthy children were recruited as a control group. The main outcome measure of HRQOL was analyzed in the two groups. We determined related factors that influenced the HRQOL in children with KHE and their parents by using a stepwise multiple regression analysis.

Results: The study found that the scores of each item in the family impact module (FIM) were lower than 75, which suggesting that KHE can make the parents of patients in a state of poor quality of life. The scores of physiological and psychosocial domains in all age groups of patients with KHE were lower than those of normal children ( < 0.01). Activity dysfunction is the factor influencing the physiological function score of all-age patients. KMP is the factor influencing the psychosocial function score of all-age patients.

Conclusions: The findings presented here suggest that patients with KHE have a poor HRQOL. KMP and activity dysfunction are risk factors for poor HRQOL in patients with KHE. However, lesion size, lesion location and education level of the mother and father were not related to the HRQOL.
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http://dx.doi.org/10.3389/fped.2022.720611DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8907831PMC
February 2022

Bibliometric Analysis Reveals a 20-Year Research Trend for Chemotherapy-Induced Peripheral Neuropathy.

Front Neurol 2021 8;12:793663. Epub 2022 Feb 8.

Department of Oncology, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, China.

Objective: A lot of research has focused on the field of chemotherapy-induced peripheral neuropathy (CIPN). In this study, we performed a bibliometric analysis of CIPN-related publications to identify the key research areas and trends over the last 20 years.

Methods: We searched the Web of Science core collection for publications related to CIPN that were published between January 2001 and September 2021. We then performed bibliometric analysis and visualization using Microsoft Excel 2019, VOSviewer, and the Bibliometric online analysis platform (https://bibliometric.com/).

Results: In total, we identified 2,188 eligible publications in the field of CIPN, with an increasing trend in the annual number of publications. The United States and Italy were dominant in the CIPN field. was the most productive journal. G. Cavaletti and A.A. Argyriou published the largest number of papers. Of all institutions, the University of Milano-Bicocca, Italy, published the highest number of papers. Analysis of the co-occurrence of keywords revealed the specific characteristics relating to the four main clusters: oxaliplatin, paclitaxel, pain management, and quality of life (QOL). Newly emerging research focusses predominantly on neuroinflammatory mechanisms and non-pharmacological interventions for CIPN.

Conclusion: This bibliometric study reviewed the evolutionary trends in CIPN research and identified current research hotspots and research trends. In addition, we identified journals, institutions, and authors, with the highest levels of impact to enhance the collaboration and learning.
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http://dx.doi.org/10.3389/fneur.2021.793663DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8860827PMC
February 2022

Significance of three-dimensional ultrasound screening of fetal micrognathia.

J Plast Reconstr Aesthet Surg 2022 04 22;75(4):1497-1520. Epub 2022 Jan 22.

Department of Plastic Surgery, Children's Hospital of Nanjing Medical University 210008, China. Electronic address:

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http://dx.doi.org/10.1016/j.bjps.2022.01.025DOI Listing
April 2022

A risk scoring system to predict the individual incidence of early-onset colorectal cancer.

BMC Cancer 2022 Jan 29;22(1):122. Epub 2022 Jan 29.

Department of Oncology, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, 100 Cross Street, Maigaoqiao, Nanjing, Jiangsu, 210028, P.R. China.

Background: The incidence of early-onset colorectal cancer (EOCRC) is increasing at an alarming rate and further studies are needed to identify risk factors and to develop prevention strategies.

Methods: Risk factors significantly associated with EOCRC were identified using meta-analysis. An individual risk appraisal model was constructed using the Rothman-Keller model. Next, a group of random data sets was generated using the binomial distribution function method, to determine nodes of risk assessment levels and to identify low, medium, and high risk populations.

Results: A total of 32,843 EOCRC patients were identified in this study, and nine significant risk factors were identified using meta-analysis, including male sex, Caucasian ethnicity, sedentary lifestyle, inflammatory bowel disease, and high intake of red meat and processed meat. After simulating the risk assessment data of 10,000 subjects, scores of 0 to 0.0018, 0.0018 to 0.0036, and 0.0036 or more were respectively considered as low-, moderate-, and high-risk populations for the EOCRC population based on risk trends from the Rothman-Keller model.

Conclusion: This model can be used for screening of young adults to predict high risk of EOCRC and will contribute to the primary prevention strategies and the reduction of risk of developing EOCRC.
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http://dx.doi.org/10.1186/s12885-022-09238-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8801093PMC
January 2022

Centimetre-scale perovskite solar cells with fill factors of more than 86 per cent.

Nature 2022 01 26;601(7894):573-578. Epub 2022 Jan 26.

School of Engineering, The Australian National University, Canberra, Australian Capital Territory, Australia.

Owing to rapid development in their efficiency and stability, perovskite solar cells are at the forefront of emerging photovoltaic technologies. State-of-the-art cells exhibit voltage losses approaching the theoretical minimum and near-unity internal quantum efficiency, but conversion efficiencies are limited by the fill factor (<83%, below the Shockley-Queisser limit of approximately 90%). This limitation results from non-ideal charge transport between the perovskite absorber and the cell's electrodes. Reducing the electrical series resistance of charge transport layers is therefore crucial for improving efficiency. Here we introduce a reverse-doping process to fabricate nitrogen-doped titanium oxide electron transport layers with outstanding charge transport performance. By incorporating this charge transport material into perovskite solar cells, we demonstrate 1-cm cells with fill factors of >86%, and an average fill factor of 85.3%. We also report a certified steady-state efficiency of 22.6% for a 1-cm cell (23.33% ± 0.58% from a reverse current-voltage scan).
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http://dx.doi.org/10.1038/s41586-021-04216-5DOI Listing
January 2022

Sirolimus plus prednisolone vs sirolimus monotherapy for kaposiform hemangioendothelioma: a randomized clinical trial.

Blood 2022 03;139(11):1619-1630

Department of Pediatric Surgery, Chengdu Women and Children's Central Hospital, Chengdu, China.

The Kasabach-Merritt phenomenon (KMP) in kaposiform hemangioendothelioma (KHE) is characterized by life-threatening thrombocytopenia and consumptive coagulopathy. This study compared the efficacy and safety of sirolimus plus prednisolone vs sirolimus monotherapy as treatment strategies for KHE with KMP in the largest cohort to date. Participants were randomized to receive either sirolimus in combination with a short course of prednisolone or sirolimus monotherapy for at least 12 months. The primary outcome was defined as achievement of a durable platelet response (platelet count >100 × 109/L) at week 4. Participants completed efficacy assessments 2 years after the initial treatment. At week 4, a durable platelet response was achieved by 35 of 37 patients given sirolimus and prednisolone compared with 24 of 36 patients given sirolimus monotherapy (difference 27.9%; 95% confidence interval, 10.0-44.7). Compared with the sirolimus monotherapy group, the combination treatment group showed improvements in terms of measures of durable platelet responses at all points during the initial 3-week treatment period, median platelet counts during weeks 1 to 4, increased numbers of patients achieving fibrinogen stabilization at week 4, and objective lesion responses at month 12. Patients receiving combination therapy had fewer blood transfusions and a lower total incidence of disease sequelae than patients receiving sirolimus alone. The frequencies of total adverse events and grade 3-4 adverse events during treatment were similar in both groups. The responses seen in patients with KHE with KMP were profound and encouraging, suggesting that sirolimus plus prednisolone should be considered a valid treatment of KHE with KMP. This trial was registered at www.clinicaltrials.gov as #NCT03188068.
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http://dx.doi.org/10.1182/blood.2021014027DOI Listing
March 2022

Solid-State NMR Dipolar and Chemical Shift Anisotropy Recoupling Techniques for Structural and Dynamical Studies in Biological Systems.

Chem Rev 2022 05 10;122(10):9880-9942. Epub 2022 Jan 10.

State Key Laboratory of Catalysis, National Laboratory for Clean Energy, 2011-Collaborative Innovation Center of Chemistry for Energy Materials, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Zhongshan Road 457, Dalian 116023, China.

With the development of NMR methodology and technology during the past decades, solid-state NMR (ssNMR) has become a particularly important tool for investigating structure and dynamics at atomic scale in biological systems, where the recoupling techniques play pivotal roles in modern high-resolution MAS NMR. In this review, following a brief introduction on the basic theory of recoupling in ssNMR, we highlight the recent advances in dipolar and chemical shift anisotropy recoupling methods, as well as their applications in structural determination and dynamical characterization at multiple time scales (i.e., fast-, intermediate-, and slow-motion). The performances of these prevalent recoupling techniques are compared and discussed in multiple aspects, together with the representative applications in biomolecules. Given the recent emerging advances in NMR technology, new challenges for recoupling methodology development and potential opportunities for biological systems are also discussed.
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http://dx.doi.org/10.1021/acs.chemrev.1c00779DOI Listing
May 2022

Simultaneous visible light optical coherence tomography and near infrared OCT angiography in retinal pathologies: A case study.

Exp Biol Med (Maywood) 2022 03 14;247(5):377-384. Epub 2021 Dec 14.

Department of Ophthalmology, 1466Johns Hopkins University, Baltimore, MD 21231, USA.

A dual-channel optical coherence tomography system with wavelengths in the visible and near-infrared light ranges can provide both structural and functional information for retinal microvasculature simultaneously. We applied this integrated system in an ongoing clinical study of patients with various retinal pathologies. Here, we present case study results of patients with diabetic retinopathy, central retinal vein occlusion, and sickle cell retinopathy compared to a healthy subject. For the first time, this comparison validates the system's ability to detect structural anomalies in both and B-scan images with simultaneous retinal optical coherence tomography angiography and measurement of sO in parafoveal vessels that are around 20-30 µm in diameter. This integrated system represents a powerful instrument with potentially far-reaching clinical implications for the early detection and diagnosis of retinal vascular diseases.
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http://dx.doi.org/10.1177/15353702211063839DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8919322PMC
March 2022

Y chemical shift anisotropy: a sensitive structural probe of layered yttrium hydroxides revealed by solid-state NMR spectroscopy and DFT calculations.

Phys Chem Chem Phys 2021 Dec 15;23(48):27244-27252. Epub 2021 Dec 15.

State Key Laboratory of Catalysis, National Laboratory for Clean Energy, 2011-Collaborative Innovation Center of Chemistry for Energy Materials, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

Anion-exchangeable Y(OH)X·HO (LYH-X, X = monovalent anions, ≈ 1.5) materials are an ideal platform for incorporating the unique properties of layered metal hydroxides and rare-earth (RE) ions, and thus have exhibited promising prospects for various applications. To further improve the performance of LYH-X and related functional materials, their structure-property relationships must be explored. However, due to the intrinsic felxibility, extracting the local structural details of these materials is particularly challenging. In this work, we utilized a combined approach of Y solid-state NMR (ssNMR) spectroscopy and density functional theory (DFT) calculations to reveal the response of Y chemical shift anisotropy (CSA) in LYH-X to the structural changes including a small displacement of cationic yttrium hydroxide layers and intercalated anions. Such subtle structural changes are often associated with dehydration/rehydration, anion-exchange, exfoliation, and the self-assembly process of LYH-X and related functional materials, which are exceedingly difficult to detect using other techniques. The principal components of Y CSA show a larger variation range than isotropic chemical shifts, making CSA a more sensitive probe. In addition, it is found that the response of Y CSA to structural changes is distinct for Y sites with different local coordination environments, opening great opportunities to analyze each Y site within these materials. All these observations suggest that the strategy involving both experimental (Y ssNMR) and theoretical (DFT) approaches can be utilized to extract previously unavailable ultrafine structural information of LYH-X and related materials, and provide fruitful insights into their thorough structure-property relationships.
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http://dx.doi.org/10.1039/d1cp04247eDOI Listing
December 2021

The web-based multiplex PCR primer design software Ultiplex and the associated experimental workflow: up to 100- plex multiplicity.

BMC Genomics 2021 Nov 18;22(1):835. Epub 2021 Nov 18.

General Surgery Center, Zhujiang Hospital, Southern Medical University, 253 Industrial Boulevard, Guangzhou, 510280, Guangdong Province, China.

Background: A large number of variants have been employed in various medical applications, such as providing medication instructions, disease susceptibility testing, paternity testing, and tumour diagnosis. A high multiplicity PCR will outperform other technologies because of its lower cost, reaction time and sample consumption. To conduct a multiplex PCR with higher than 100 plex multiplicity, primers need to be carefully designed to avoid the formation of secondary structures and nonspecific amplification between primers, templates and products. Thus, a user-friendly, highly automated and highly user-defined web-based multiplex PCR primer design software is needed to minimize the work of primer design and experimental verification.

Results: Ultiplex was developed as a free online multiplex primer design tool with a user-friendly web-based interface ( http://ultiplex.igenebook.cn ). To evaluate the performance of Ultiplex, 294 out of 295 (99.7%) target primers were successfully designed. A total of 275 targets produced qualified primers after primer filtration, and 271 of those targets were successfully clustered into one compatible PCR group and could be covered by 108 primers. The designed primer group stably detected the rs28934573(C > T) mutation at lower than a 0.25% mutation rate in a series of samples with different ratios of HCT-15 and HaCaT cell line DNA.

Conclusion: Ultiplex is a web-based multiplex PCR primer tool that has several functions, including batch design and compatibility checking for the exclusion of mutual secondary structures and mutual false alignments across the whole genome. It offers flexible arguments for users to define their own references, primer Tm values, product lengths, plex numbers and tag oligos. With its user-friendly reports and web-based interface, Ultiplex will provide assistance for biological applications and research involving genomic variants.
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http://dx.doi.org/10.1186/s12864-021-08149-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8600765PMC
November 2021

Hematemesis in an Infant: A Rare Tumor in a Rare Site.

Gastroenterology 2022 06 30;162(7):e3-e5. Epub 2021 Oct 30.

Division of Oncology, Department of Pediatric Surgery, West China Hospital of Sichuan University, Chengdu, China. Electronic address:

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http://dx.doi.org/10.1053/j.gastro.2021.10.033DOI Listing
June 2022

Treatment of Syndromic Craniosynostosis by Anterior and Posterior Vault Distraction Osteogenesis (A-PVDO).

J Craniofac Surg 2022 Mar-Apr 01;33(2):654-656

Department of Plastic Surgery, Children's Hospital of Nanjing Medical University, Nanjing 210008, China.

Objective: To explore the feasibility and therapeutic effect of anterior and posterior vault distraction osteogenesis (A-PVDO) in the treatment of infantile syndromic craniosynostosis.

Methods: Between January 2017 and December 2019, 7 children with syndromic craniosynostosis underwent osteotomy with Piezo-surgery at our hospital. The first step was to harvest the frontal bone flap and the orbitofrontal bone flap. The second step was to separate the scalp and expose the posterior occipital. Osteotomy was performed on the occipital tubercle. Thereafter, 2 distractors were horizontally installed on the upper edge of the anterior cranial orbit, 2 distractors of 3 cm were installed on the posterior cranial bone. Meanwhile, lambdoidal sutures were fixed by titanium plates. Bone distraction was initiated on postoperative day 5 at the rate of 0.4 to 0.6 mm/day, twice per-day, for a total of 10 to 15 days. After 6 months, the distractors and the titanium plates were removed by secondary surgery.

Results: The intracranial volume and posterior cranial morphology were recorded during the follow-up of 6 to 14 months (average = 12 months). The posterior craniums of 7 cases with lambdoidal sutures fixation were completely extended. The anterior cranial morphology was normal. All the cranial deformities were significantly improved. There were no severe complications, such as death, cranial necrosis, and intracranial infection.

Conclusions: A-PVDO is an ideal method for the treatment of severe syndromic craniosynostosis, which can achieve more natural appearance than anterior vault distraction osteogenesis or posterior vault distraction osteogenesis. Moreover, A-PVDO causes no severe complications and is suitable for the treatment of severe syndromic craniosynostosis.
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http://dx.doi.org/10.1097/SCS.0000000000008289DOI Listing
April 2022

Inflow Occlusion Combined With Bleomycin Sclerotherapy for Management of Macro/Mixed Cystic Lymphatic Malformation in Children.

Front Pediatr 2021 22;9:749983. Epub 2021 Sep 22.

Department of Burns and Plastic Surgery, Children's Hospital of Nanjing Medical University, Nanjing, China.

The link between cystic lymphatic malformation (cLM) and normal lymphatic system has become the focus of research. This study aimed to assess the outcomes of indocyanine green (ICG) lymphography-guided inflow occlusion combined with bleomycin sclerotherapy for the management of macro or mixed cLM in children. Between June 2018 and October 2020, inflow occlusion combined with bleomycin sclerotherapy was performed in 81 cLM patients (age range from 6 months to 8 years). All cases were evaluated by the following parameters: cLM location, histological typing, number of afferent lymph vessels, dermal backflow, curative effects, treatment frequency, and postoperative complications. The duration of postoperative follow-up was from 10 to 16 months. All cLM cases could be found with at least one lymphatic inflow. Excellent outcomes were observed in 68 cases (84.0%), 11 cases (13.6%) experienced good outcomes, and two (2.5%) cases had fair outcome. No case experienced repeated treatment for more than three times. Wound infection, fever, and scar hyperplasia were the independent adverse events, which were managed by symptomatic treatment. Inflow occlusion combined with bleomycin sclerotherapy renders a safe and efficient approach for the management of macro or mixed cLM.
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http://dx.doi.org/10.3389/fped.2021.749983DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8493213PMC
September 2021

Preventive Efficacy and Safety of Yiqi-Wenjing-Fang Granules on Oxaliplatin-Induced Peripheral Neuropathy: A Protocol for a Randomized, Double-Blind, Placebo-Controlled, Multicenter Trial.

Evid Based Complement Alternat Med 2021 29;2021:5551568. Epub 2021 Sep 29.

The Third Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210046, China.

. Oxaliplatin-induced peripheral neuropathy (OIPN) is one of the most common side effects of oxaliplatin, which can cause reduction and cessation of oxaliplatin-based chemotherapy and significantly affect patients' quality of life. However, no drug has got recognition to prevent or treat OIPN. Yiqi-Wenjing-Fang (YWF) is a joint name of Chinese medicine prescriptions with similar effects of tonifying qi and warming meridians, represented by Huangqi Guizhi Wuwu decoction (HGWD) and Danggui Sini decoction (DSD), both from "Treatise on Cold Pathogenic and Miscellaneous Diseases." YWF granules, including HGWD granules and DSD granules, have been, respectively, demonstrated to be effective in preventing OIPN in previous small-sample observations. The purpose of this study is to enlarge the sample size for further evaluation of the preventive efficacy and safety of YWF granules on OIPN. This study is a randomized, double-blind, placebo-controlled, and multicenter clinical trial. 360 postoperative patients with stage IIa-IIIc colorectal cancer will be randomly assigned into placebo-control group, intervention group I, and intervention group II, taking the mimetic granules of YWF as placebo, HGWD granules and DSD granules, respectively. All subjects will receive oxaliplatin-based chemotherapy regimen at the same time. EORTC QLQ-CIPN20 will be used to assess the degree of OIPN as the primary outcome measure. The grades of OIPN, quality of life, chemotherapeutic efficacy, and the number of completed chemotherapy cycles are selected as the secondary outcome measures. . Based on the condition of no recognized effective drugs in preventing OIPN, evidence-based medical study will be conducted for seeking a breakthrough in the field of Chinese herb medicine. This protocol could provide reliable and systemic research basis about the efficacy of YWF granules and the differentiation of two classical prescriptions of YWF on preventing OIPN objectively. This study was registered at ClinicalTrials.gov on 26 December 2020 (ID: https://clinicaltrials.gov/ct2/show/NCT04690283).
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http://dx.doi.org/10.1155/2021/5551568DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8494586PMC
September 2021

Accurate heteronuclear distance measurements at all magic-angle spinning frequencies in solid-state NMR spectroscopy.

Chem Sci 2021 Sep 20;12(34):11554-11564. Epub 2021 Jul 20.

State Key Laboratory of Catalysis, National Laboratory for Clean Energy, 2011-Collaborative Innovation Center of Chemistry for Energy Materials, Dalian Institute of Chemical Physics, Chinese Academy of Sciences Zhongshan Road 457 Dalian 116023 China

Heteronuclear dipolar coupling is indispensable in revealing vital information related to the molecular structure and dynamics, as well as intermolecular interactions in various solid materials. Although numerous approaches have been developed to selectively reintroduce heteronuclear dipolar coupling under MAS, most of them lack universality and can only be applied to limited spin systems. Herein, we introduce a new and robust technique dubbed phase modulated rotary resonance (PMRR) for reintroducing heteronuclear dipolar couplings while suppressing all other interactions under a broad range of MAS conditions. The standard PMRR requires the radiofrequency (RF) field strength of only twice the MAS frequency, can efficiently recouple the dipolar couplings with a large scaling factor of 0.50, and is robust to experimental imperfections. Moreover, the adjustable window modification of PMRR, dubbed wPMRR, can improve its performance remarkably, making it well suited for the accurate determination of dipolar couplings in various spin systems. The robust performance of such pulse sequences has been verified theoretically and experimentally model compounds, at different MAS frequencies. The application of the PMRR technique was demonstrated on the H-ZSM-5 zeolite, where the interaction between the Brønsted acidic hydroxyl groups of H-ZSM-5 and the absorbed trimethylphosphine oxide (TMPO) were probed, revealing the detailed configuration of super acid sites.
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http://dx.doi.org/10.1039/d1sc03194eDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8409495PMC
September 2021
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