Publications by authors named "Yi Huang"

1,657 Publications

  • Page 1 of 1

Efficacy and safety of adalimumab in comparison to infliximab for Crohn's disease: A systematic review and meta-analysis.

World J Clin Cases 2022 Jun;10(18):6091-6104

Department of Endocrinology, Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430077, Hubei Province, China.

Background: Adalimumab (ADA) and infliximab (IFX) are the cornerstones of the treatment of Crohn's disease (CD). It remains controversial whether there is a difference in the effectiveness and safety between IFX and ADA for CD.

Aim: To perform a meta-analysis to compare the effectiveness and safety of ADA and IFX in CD.

Methods: PubMed, Embase, Cochrane Library, and Web of Science databases were searched. Cohort studies were considered for inclusion. The primary outcomes were induction of response and remission, maintenance of response and remission, and secondary loss of response. Adverse events were secondary outcomes.

Results: Fourteen cohort studies were included. There was no apparent difference between the two agents in the induction response [odds ratio (OR): 1.27, 95% confidence interval (CI): 0.93-1.74, = 0.14] and remission (OR: 1.11, 95%CI: 0.78-1.57, = 0.57), maintenance response (OR: 1.08, 95%CI: 0.76-1.53, = 0.67) and remission (OR: 1.26, 95%CI: 0.87-1.82, = 0.22), and secondary loss of response (OR: 1.01, 95%CI: 0.65-1.55, = 0.97). Subgroup analysis revealed ADA and IFX had similar rates of response, remission, and loss of response either in anti-tumor necrosis factor-α naïve or non-naïve patients. Further, there was a similar result regardless of whether CD patients were treated with optimized therapy, including dose intensification, shortening interval, and combination immunomodulators. However, ADA had a fewer overall adverse events than IFX (OR: 0.62, 95%CI: 0.42-0.91, = 0.02).

Conclusion: ADA and IFX have similar clinical benefits for anti-tumor necrosis factor-α naïve or non-naïve CD patients. Overall adverse events rate is higher in patients in the IFX group.
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http://dx.doi.org/10.12998/wjcc.v10.i18.6091DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9254215PMC
June 2022

Meta-Analysis of Predictive Role of Early Neurological Deterioration after Intravenous Thrombolysis.

Emerg Med Int 2022 22;2022:2894426. Epub 2022 Jul 22.

Department of Neurology, Hubei No. 3 People's Hospital of Jianghan University, Wuhan 430033, Hubei Province, China.

With the popularization of intravenous thrombolysis, more and more people use intravenous thrombolysis to treat related diseases, but problems also arise. There are still a considerable number of patients with early disease after thrombolytic therapy not only not significantly improving, but also progressing, that is, early neurological deterioration (END). In view of this problem, the prediction of END after intravenous thrombolysis becomes very important. With the development of medical technology, research on the prediction of END after intravenous thrombolysis has gradually been carried out. Effective prediction is of great significance for the prevention and treatment of END after intravenous thrombolysis. This article aimed to carry out a meta-analysis of the predictive role of END after intravenous thrombolysis. Through an informed analysis of all studies of this type in this field, this article determines a method for predicting END after intravenous thrombolysis. The actual effect of its role is revealed in this paper, and its purpose is to promote the development of this field. This article addresses the same type of study on the predictive role of neurological deterioration after intravenous thrombolysis. The article performs test and meta-analysis of its role by conditionally searching for literature studies. It is explained using the relevant theoretical formulas. The analysis results show that the prediction of END after intravenous thrombolysis in this paper can effectively help make a preliminary judgment on the possible later neurological deterioration. Although there is an error between the predicted curve and the actual curve, the difference between the two is between 1% and 5%. It can basically effectively predict the occurrence of END. Therefore, the prediction of END after intravenous thrombolysis has a very large preventive effect on the END after intravenous thrombolysis.
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http://dx.doi.org/10.1155/2022/2894426DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9337960PMC
July 2022

A "3S+f" Nephrometry Score System to Predict the Clinical Outcomes of Laparoscopic Nephron-Sparing Surgery.

Front Oncol 2022 14;12:922082. Epub 2022 Jul 14.

Department of Urology, Peking University Third Hospital, Peking University, Beijing, China.

Background: When we treat renal cell carcinoma by laparoscopic nephron-sparing surgery (NSS), it is essential to use an evaluation system to predict clinical outcomes. Hitherto, there are more than a dozen nephrometry score systems. In this study, through assessing the correlations between nephrometry score systems and clinical outcomes, we aim to provide a novel nephrometry score system-the "3S+f" score system-to simplify the evaluation of technical complexity of partial nephrectomy.

Methods: We retrospectively collected the data of 131 patients who underwent NSS, which was performed by a single surgeon (SZ) from January 2013 to July 2018 at Peking University Third Hospital. The "3S+f" score system contains four parameters: "size, side, site, and fat", all of which can be obtained from preoperative imaging data. We evaluated the correlations between the "3S+f" score and clinical outcomes, and compared R.E.N.A.L. score and PADUA score.

Results: All the three nephrometry score systems were related to some clinical outcomes in univariate analyses. In multivariate regression models, the "3S+f" score, the R.E.N.A.L. score, and the PADUA score were significantly associated with operative time ( = 0.016, = 0.035, and = 0.001, respectively) and warm ischemia time (all = 0.008, < 0.001, and < 0.001, respectively). "3S+f" was also significantly related to extubation time > 5 days ( = 0.018). In predicting operative time > 120 min and extubation time >5 days from ROC curves, the AUCs of the "3S+f" score (0.717 and 0.652, respectively) were larger than both the R.E.N.A.L (0.598 and 0.554, respectively) and PADUA (0.600 and 0.542, respectively) score systems.

Conclusion: A novel nephrometry score system-the "3S+f" score system-shows equivalent correlation and the ability in predicting clinical outcomes when compared to the R.E.N.A.L. score system and the PADUA score system, which can describe renal tumors.
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http://dx.doi.org/10.3389/fonc.2022.922082DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330399PMC
July 2022

Cavernous sinus dural arteriovenous fistula complicated by hippocampus venous congestion: A case report.

J Stroke Cerebrovasc Dis 2022 Jul 27;31(9):106666. Epub 2022 Jul 27.

Department of Neurosurgery, Ningbo First Hospital, Ningbo Hospital, Zhejiang University School of Medicine, Ningbo, Zhejiang 315010, China. Electronic address:

Background: Hippocampal venous congestion is a rare complication associated with cavernous sinus dural arteriovenous fistulas (CS-DAVFs).

Case Description: A 74-year-old woman was admitted to the hospital with a swollen left eye. Isolated lesions were found in the left hippocampus and the middle cerebellar peduncle. Cerebral angiography revealed retrograde venous drainage of the bilateral inferior petrosal sinuses from the left CS-DAVF. The patient underwent transcatheter arterial embolization, resulting in complete resolution of the hippocampal lesions and neurological symptoms.

Conclusion: Hippocampal injury is a rare complication of CS-DAVF. Attentive diagnosis and treatment can effectively prevent adverse consequences.
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http://dx.doi.org/10.1016/j.jstrokecerebrovasdis.2022.106666DOI Listing
July 2022

DNA Methylation of Patatin-Like Phospholipase Domain-Containing Protein 6 Gene Contributes to the Risk of Intracranial Aneurysm in Males.

Front Aging Neurosci 2022 11;14:885680. Epub 2022 Jul 11.

Department of Neurosurgery, Ningbo First Hospital, Ningbo, China.

: This study is aimed to investigate the contribution of patatin-like phospholipase domain-containing protein 6 () DNA methylation to the risk of intracranial aneurysm (IA) in the Han Chinese population. : A total of 96 age- and sex-matched participants were recruited to evaluate methylation bisulfite pyrosequencing. The mRNA expression in the plasma was determined using real-time quantitative reverse transcription-polymerase chain reaction. Human primary artery smooth muscle cells (HPCASMC) were used for the in vitro function study. : methylation was significantly higher in patients with IA than in healthy controls ( < 0.01). Sex group analysis showed that this correlation appeared in the male group ( < 0.01) but not in the female group ( > 0.05). methylation was significantly associated with age in all participants ( = 0.306, = 0.003) and in the control group ( = 0.377, = 0.008) but not in the IA group ( = 0.127, = 0.402). Furthermore, the mRNA expression significantly decreased in patients with IA than that in the controls ( = 0.016). expression was significantly inversely correlated with elevated DNA methylation in participants (r = -0.825, < 0.0001). In addition, transcription was significantly enhanced following treatment with 5-aza-2'-deoxycytidine methylation inhibitor in HPCASMC.The receiver operating characteristic analyses of curves showed that the mean methylation [area under the curve (AUC) = 0.74, < 0.001] and mRNA expression (AUC = 0.86, < 0.001) could have a diagnostic value for patients with IA. : Although future functional experiments are required to test our hypothesis, our study demonstrated that methylation and mRNA expression were significantly associated with the risk of IA; thus, they show potential for use in the early diagnosis of IA.
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http://dx.doi.org/10.3389/fnagi.2022.885680DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309567PMC
July 2022

Post-tuberculosis tracheobronchial stenosis: long-term follow-up after self-expandable metallic stents placement and development of a prediction score-the Restenosis Score.

Eur J Med Res 2022 Jul 27;27(1):133. Epub 2022 Jul 27.

Department of Respiratory and Critical Care Medicine, Shanghai Changhai Hospital, The First Affiliated Hospital of Second Military Medical University, Shanghai, 200433, China.

Background: The insertion of self-expandable metallic stents (SEMS) for post-tuberculosis tracheobronchial stenosis (PTTS) was controversial. This study aimed to evaluate the efficacy and safety of SEMS for treating PTTS, and developed a scoring system for predicting the occurrence of restenosis after stenting in PTTS patients.

Methods: We conducted a retrospective review of 87 patients who were diagnosed with PTTS and experienced SEMS insertion between January 2000 and December 2017. All procedures were performed via flexible bronchoscopy under conscious sedation and local anesthesia.

Results: A total of 85 SEMS were successfully placed in 77 patients. Comparing with pre-stenting, there were significant improvements in the lumen diameters of the stenotic segment, mMRC scale and lung function after short-term SEMS placement. During the long-term (average 163.32 months) follow-up, 48 patients (62.3%) did not develop restenosis after stenting; the other 29 patients (37.7%) developed and eventually, 12 remained under interventional therapies and 11 had bronchial atresia. Multivariate Cox regression analysis revealed that the difference value between SEMS length and the stenosis-segment length, stenosis type, and the number of pre-stenting thermal ablation were independently related to restenosis occurrence and were subsequently used to establish the Restenosis Score. The model's development group (0.83, 95% CI 0.74-0.92) and external validation set (0.94, 95% CI 0.77-1.00) showed excellent discrimination.

Conclusion: SEMS placement could serve as a safe and effective treatment option for most patients with PTTS. Further, we built a prediction model depending on the independent predictors of restenosis occurrence, the Restenosis Score. This validated tool might provide a decision support and a better management for PTTS patients who underwent SEMS implantation.
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http://dx.doi.org/10.1186/s40001-022-00765-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9327164PMC
July 2022

A Novel Necroptosis-Related Prognostic Signature of Glioblastoma Based on Transcriptomics Analysis and Single Cell Sequencing Analysis.

Brain Sci 2022 Jul 26;12(8). Epub 2022 Jul 26.

Department of Neurosurgery, Ningbo First Hospital, Zhejiang University School of Medicine, Ningbo 315010, China.

Background: Glioblastoma (GBM) is the most common and deadly brain tumor. The clinical significance of necroptosis (NCPS) genes in GBM is unclear. The goal of this study is to reveal the potential prognostic NCPS genes associated with GBM, elucidate their functions, and establish an effective prognostic model for GBM patients.

Methods: Firstly, the NCPS genes in GBM were identified by single-cell analysis of the GSE182109 dataset in the GEO database and weighted co-expression network analysis (WGCNA) of The Cancer Genome Atlas (TCGA) data. Three machine learning algorithms (Lasso, SVM-RFE, Boruta) combined with COX regression were used to build prognostic models. The subsequent analysis included survival, immune microenvironments, and mutations. Finally, the clinical significance of NCPS in GBM was explored by constructing nomograms.

Results: We constructed a GBM prognostic model composed of NCPS-related genes, including CTSD, AP1S1, YWHAG, and IER3, which were validated to have good performance. According to the above prognostic model, GBM patients in the TCGA and CGGA groups could be divided into two groups according to NCPS, with significant differences in survival analysis between the two groups and a markedly worse prognostic status in the high NCPS group ( <0.001). In addition, the high NCPS group had higher levels of immune checkpoint-related gene expression, suggesting that they may be more likely to benefit from immunotherapy.

Conclusions: Four genes (CTSD, AP1S1, YWHAG, and IER3) were screened through three machine learning algorithms to construct a prognostic model for GBM. These key and novel diagnostic markers may become new targets for diagnosing and treating patients with GBM.
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http://dx.doi.org/10.3390/brainsci12080988DOI Listing
July 2022

Puerarin Attenuates Oxidative Stress and Ferroptosis via AMPK/PGC1α/Nrf2 Pathway after Subarachnoid Hemorrhage in Rats.

Antioxidants (Basel) 2022 Jun 27;11(7). Epub 2022 Jun 27.

Department of Neurosurgery, Ningbo First Hospital, Ningbo Hospital, Zhejiang University School of Medicine, Ningbo 315010, China.

Puerarin was shown to exert anti-oxidative and anti-ferroptosis effects in multiple diseases. The goal of this study was to explore the neuroprotective effect of puerarin on early brain injury (EBI) after subarachnoid hemorrhage (SAH) in rats. A total of 177 adult male Sprague Dawley rats were used. SAH was included via endovascular perforation. Intranasal puerarin or intracerebroventricular dorsomorphin (AMPK inhibitor) and SR18292 (PGC1α inhibitor) were administered. The protein levels of pAMPK, PGC1α, Nrf2, 4HNE, HO1, MDA, ACSL4, GSSG, and iron concentration in the ipsilateral hemisphere were significantly increased, whereas SOD, GPX4, and GSH were decreased at 24 h after SAH. Moreover, puerarin treatment significantly increased the protein levels of pAMPK, PGC1α, Nrf2, HO1, SOD, GPX4, and GSH, but decreased the levels of 4HNE, MDA, ACSL4, GSSG, and iron concentration in the ipsilateral hemisphere at 24 h after SAH. Dorsomorphin or SR18292 partially abolished the beneficial effects of puerarin exerted on neurological dysfunction, oxidative stress injury, and ferroptosis. In conclusion, puerarin improved neurobehavioral impairments and attenuated oxidative-stress-induced brain ferroptosis after SAH in rats. The neuroprotection acted through the activation of the AMPK/PGC1α/Nrf2-signaling pathway. Thus, puerarin may serve as new therapeutics against EBI in SAH patients.
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http://dx.doi.org/10.3390/antiox11071259DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312059PMC
June 2022

Role of Pyroptosis in Gynecological Oncology and Its Therapeutic Regulation.

Biomolecules 2022 07 1;12(7). Epub 2022 Jul 1.

The Reproductive Medicine Center, The 1st Hospital of Lanzhou University, Lanzhou 730000, China.

With the continuous advances in molecular biotechnology, many new cell death methods have been discovered. Pyroptosis is a programmed cell death process that differs from apoptosis and autophagy in cell morphology and function. Compared with apoptosis and autophagy, pyroptosis is primarily mediated by intracellular inflammasome and gasdermin D of the gasdermin protein family and involves the release of numerous inflammatory factors. Pyroptosis has been found to be involved in the occurrence and development of infectious diseases and other diseases involving the nervous system and the cardiovascular system. Recent studies have also reported the occurrence of pyroptosis in tumor cells. Accordingly, exploring its effect on tumors has become one of the research hotspots. Herein, recent research progress on pyroptosis is reviewed, especially its role in the development of gynecological tumors. As the pathogenesis of gynecological tumor is better understood, new targets have been introduced for the prevention and clinical treatment of gynecological tumors.
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http://dx.doi.org/10.3390/biom12070924DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313147PMC
July 2022

Evaluating nicotinamide adenine dinucleotide for its effects on halo blight of snap bean.

Plant Dis 2022 Jul 26. Epub 2022 Jul 26.

University of Florida, Tropical REC, 18905 SW 280th Street, Homestead, Florida, United States, 33031;

Halo blight caused by pv. is one of the major bacterial diseases on snap bean in Florida, and the outbreaks of this disease have occurred more often in recent years. Current management of this disease primarily depends on application of fixed copper-based bactericides, but the climate change and resistance development in the pathogen populations still cause hardship for disease management especially in south Florida. In this study, nicotinamide adenine dinucleotide (NAD) was evaluated for its potential to reduce halo blight on snap bean. When NAD at 5 mM was applied by soil drench, foliar spray, or leaf infiltration, NAD significantly (P < 0.05) reduced disease severity of halo blight on snap bean compared to the untreated control. When NAD was applied by leaf infiltration, among the tested concentrations, NAD at 0.5-1.0 mM was most effective in decreasing halo blight disease. NAD at 2.5 mM applied as a foliar spray in rotation with Kocide 3000 (copper hydroxide) at 0.5 mg/ml further reduced the disease severity compared to Kocide 3000 alone. In the in-vitro study, no inhibitory effects of NAD+ were detected on the pathogen P. syringae pv. phaseolicola. Results of real-time polymerase chain reaction (qPCR) showed that the defense-related genes , , , , , and were up-regulated in the NAD treatment. Taken together, these data indicate that NAD significantly suppressed halo blight on snap bean, and application of NAD has the potential in management of this important disease.
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http://dx.doi.org/10.1094/PDIS-05-22-1126-REDOI Listing
July 2022

The KIR2DL2/HLA-C1C1 Gene Pairing Is Associated With an Increased Risk of SARS-CoV-2 Infection.

Front Immunol 2022 7;13:919110. Epub 2022 Jul 7.

Wuhan Institute of Biomedical Sciences, School of Medicine, Jianghan University, Wuhan, China.

SARS-CoV-2 is the causative agent for the global COVID-19 pandemic; however, the interaction between virus and host is not well characterized. Natural killer cells play a key role in the early phase of the antiviral response, and their primary functions are dependent on signaling through the killer cell immunoglobulin-like receptor (KIR). This study measured the association between KIR/HLA class I ligand pairings and the occurrence and development of COVID-19. DNA of blood samples from 257 COVID-19 patients were extracted and used to detect KIR and HLA-C gene frequencies using single strain sequence-specific primer (SSP) PCR. The frequency of these genes was compared among 158 individuals with mild COVID-19, 99 with severe disease, and 98 healthy controls. The frequencies of KIR2DL2 (P=0.04, OR=1.707), KIR2DS3 (P=0.047, OR=1.679), HLA-C1C1 (P<0.001, OR=3.074) and the KIR2DL2/HLA-C1C1 pairing (P=0.038, OR=2.126) were significantly higher in the COVID-19 patients than the healthy controls. At the same time, the frequency of KIR2DL3+KIR2DL2-/HLA-C1+Others+ was lower in COVID-19 patients than in healthy individuals (P=0.004, OR=0.477). These results suggest that the protective effect of KIR2DL3 against SARS-CoV-2 infection is related to the absence of the KIR2DL2 gene. This study found no correlation between the frequencies of these genes and COVID-19 pathogenesis. Global statistical analysis revealed that the incidence of COVID-19 infection was higher in geographic regions with a high frequency of KIR2DL2. Together these results suggest that the KIR2DL2/HLA-C1C1 gene pairing may be a risk factor for SARS-CoV-2 infection.
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http://dx.doi.org/10.3389/fimmu.2022.919110DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301464PMC
July 2022

Association Between Plasma Fibulin-1 and Brachial-Ankle Pulse Wave Velocity in Arterial Stiffness.

Front Cardiovasc Med 2022 7;9:837490. Epub 2022 Jul 7.

Department of Geriatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Arterial stiffness forms the basis of cardiovascular diseases (CVD) and is also an independent predictor of CVD risk. Early detection and intervention of arterial stiffness are important for improving the global burden of CVD. Pulse wave velocity (PWV) is the gold standard for assessing arterial stiffness and the molecular mechanism of arterial stiffness remains to be studied. Extracellular matrix (ECM) remodeling is one of the major mechanisms of arterial stiffness. Partial quantitative changes of ECM proteins can be detected in plasma. Therefore, we examined the hypothesis that a discovery proteomic comparison of plasma proteins between high arterial stiffness (baPWV ≥ 1,400 cm/s) and normal arterial stiffness (baPWV < 1,400 cm/s) populations might identify relevant changed ECM proteins for arterial stiffness. Plasma samples were randomly selected from normal arterial stiffness ( = 6) and high arterial stiffness ( = 6) people. Isobaric tags for relative and absolute quantitation (iTRAQ) based quantitative proteomics technique was performed to find a total of 169 differentially expressed proteins (DEPs). Nine ECM proteins were included in all DEPs and were all up-regulated proteins. Fibulin-1 had the highest statistically fold-change (FC = 3.7, < 0.0001) in the high arterial stiffness population compared with the control group during the nine ECM proteins. The expression of plasma fibulin-1 in normal arterial stiffness ( = 112) and high arterial stiffness ( = 72) populations was confirmed through enzyme-linked immunosorbent assay (ELISA). Similarly, ELISA results showed that plasma concentrations of fibulin-1 in the high arterial stiffness group were higher than those in the normal arterial stiffness group (12.69 ± 0.89 vs. 9.84 ± 0.71 μg/ml, < 0.05). Univariate analysis of fibulin-1 with brachial-ankle pulse wave velocity (baPWV) indicated that fibulin-1 was positively correlated with baPWV in all participants ( = 0.32, < 0.01) and a stronger positive correlation between baPWV and fibulin-1 in high arterial stiffness group ( = 0.64, < 0.0001) was found. Multiple regression analysis of factors affecting baPWV showed that fibulin-1 was also a significant determinant of the increased ba-PWV ( = 0.635, = 0.001). Partial correlation analysis showed that baPWV increased with the growth of plasma fibulin-1( = 0.267, < 0.001). In conclusion, our results demonstrated that fibulin-1 is positively correlated with ba-PWV and an independent risk factor for arterial stiffness.
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http://dx.doi.org/10.3389/fcvm.2022.837490DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302601PMC
July 2022

Selective catalytic oxidation of formaldehyde on single V- and Cr-atom decorated magnetic CN substrate: A first principles study.

J Hazard Mater 2022 Jul 14;439:129608. Epub 2022 Jul 14.

School of Energy and Power Engineering, Jiangsu University of Science and Technology, Zhenjiang 212003, China.

Formaldehyde (HCHO) is the most common indoor hazardous pollutant and has attracted great concern because its long-term exposure has adverse health effects on humans. Retention and catalytic oxidation of highly hazardous HCHO is an efficient and environmentally friendly method to use for air remediation, but a major obstacle to this procedure is the lack of an appropriate catalyst. Herein, two-dimensional magnetic CN material with a 3d-transition metal as activate sites was systemically investigated in HCHO oxidation using density functional theory calculations. The results show that V-CN and Cr-CN have high structural stability and shallow activation barriers for O decomposition; these characteristics provide the necessary precursors for the subsequent oxidation reaction. Moreover, the V-CN and Cr-CN catalysts have unique selective adsorption and catalysis toward HCHO in a mixture of some typical in-door volatile organic compounds (VOCs) and air. The corresponding dynamic barrier for each reaction step was investigated and the mechanism involved in HCHO oxidation was revealed in detail. Aggregation of metal atoms in the V-CN and Cr-CN catalysts is prevented by enormous diffusion resistance, and this is further confirmed by AIMD simulations. These results provide insightful guidance for developing advanced magnetic catalysts for HCHO oxidation to improve the remediation of air contaminants.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129608DOI Listing
July 2022

Biodegradable microplastics enhance soil microbial network complexity and ecological stochasticity.

J Hazard Mater 2022 Jul 14;439:129610. Epub 2022 Jul 14.

Beijing Key Laboratory of Farmland Soil Pollution Prevention and Remediation, College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China. Electronic address:

Biodegradable plastics have emerged as an ecological alternative to conventional petroleum-based plastics. Despite the recent advances in the effects of conventional microplastic on soil ecosystems, the ecological impact of biodegradable microplastics in soil environments remains poorly understood. Here, we performed soil microcosms with conventional (polyethylene and polystyrene) and biodegradable (polybutylene succinate and polylactic acid) microplastics to estimate their effects on the success patterns, co-occurrence networks, and the assembly mechanisms of soil bacterial communities. Biodegradable microplastics significantly altered the soil bacterial community composition with steeper temporal turnovers (rate: 0.317 - 0.514) compared to the conventional microplastic treatments (rate: 0.211 - 0.220). Network under biodegradable microplastics showed greater network complexity, including network size, connectivity, average clustering coefficient, and the number of keystone species, as compared with the conventional microplastic treatments. Additionally, the biodegradable microplastic network had higher robustness, which may be potentially due to the enhanced dissolved organic carbon contents in the soil treated with biodegradable microplastics. The bacterial community assembly was initially governed by deterministic homogeneous selection (93 - 100 %) under the stress of microplastics, but was progressively structured by increasing stochastic homogeneous dispersal (17.8 - 73.3 %) over time. The normalized stochasticity ratio also revealed that the application of microplastics increased the importance of stochastic processes following incubation. These findings greatly enhanced our understanding of the ecological mechanisms and interactions of soil bacterial communities in response to microplastic stress.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129610DOI Listing
July 2022

Occupational Therapy and Prevention of Common Sports Injuries for Special Physical Training.

Occup Ther Int 2022 5;2022:6227377. Epub 2022 Jul 5.

School of Sports and Art, Hunan University of Medicine, Huaihua Hunan 418000, China.

This paper provides an in-depth study of occupational therapy and the prevention of common sports injuries in special physical training. The issue of sports injuries and rehabilitation has always been a hot topic in special training. With the continuous development of sports, the increasing intensity of competition, and more stringent requirements for special techniques, the increase in difficulty and intensity of training has led to the increasing frequency of sports injuries, so how to prevent injuries in special physical training and rehabilitation and recovery of athletes after the injury is particularly important. Since the most common musculoskeletal injuries occur in the lower quadrant, this paper proposes a lower extremity functional test (LEFT) model as a means of identifying injury risk and guiding the implementation of training programs to prevent sports injuries. In this paper, a knee injury is used as an example, and an occupational therapy program of TCM physical therapy + aquatic rehabilitation is adopted for the already occurred sports injuries. Through interviews and clinical examinations of athletes, coaches, and medical personnel, this paper summarizes the sites, types, characteristics, and probability of occurrence of common sports injuries in special physical training. Experiments were conducted through clinical rehabilitation of common sports injuries with the addition of TCM manual massage. A series of effects of this modality on the rehabilitation of sports injuries were examined by monitoring physiological and biochemical indexes and by comparative analysis before and after testing physical function indexes using the Omega Wave system. Sports injuries are diverse. Traditional Chinese medicine physical therapy + water rehabilitation therapy is an effective physical therapy method. According to the relevant theories of traditional Chinese medicine treatment, diagnosis and treatment through meridians and related acupuncture points have significant curative effects. Traditional Chinese medicine, massage, and acupuncture have irreplaceable roles in the rehabilitation and treatment of sports injuries and can effectively improve and cure sports injuries.
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http://dx.doi.org/10.1155/2022/6227377DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277208PMC
July 2022

Biological Effects and Mechanisms of Caspases in Early Brain Injury after Subarachnoid Hemorrhage.

Oxid Med Cell Longev 2022 5;2022:3345637. Epub 2022 Jul 5.

Department of Neurosurgery, Ningbo First Hospital, Ningbo Hospital, Zhejiang University School of Medicine, Ningbo, Zhejiang 315010, China.

Caspases are an evolutionarily conserved family of proteases responsible for mediating and initiating cell death signals. In the past, the dysregulated activation of caspases was reported to play diverse but equally essential roles in neurodegenerative diseases, such as brain injury and neuroinflammatory diseases. A subarachnoid hemorrhage (SAH) is a traumatic event that is either immediately lethal or induces a high risk of stroke and neurological deficits. Currently, the prognosis of SAH after treatment is not ideal. Early brain injury (EBI) is considered one of the main factors contributing to the poor prognosis of SAH. The mechanisms of EBI are complex and associated with oxidative stress, neuroinflammation, blood-brain barrier disruption, and cell death. Based on mounting evidence, caspases are involved in neuronal apoptosis or death, endothelial cell apoptosis, and increased inflammatory cytokine-induced by apoptosis, pyroptosis, and necroptosis in the initial stages after SAH. Caspases can simultaneously mediate multiple death modes and regulate each other. Caspase inhibitors (including XIAP, VX-765, and Z-VAD-FMK) play an essential role in ameliorating EBI after SAH. In this review, we explore the related pathways mediated by caspases and their reciprocal regulation patterns after SAH. Furthermore, we focus on the extensive crosstalk of caspases as a potential area of research on therapeutic strategies for treating EBI after SAH.
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http://dx.doi.org/10.1155/2022/3345637DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277153PMC
July 2022

Integration Profiling Between Plasma Lipidomics, Epstein-Barr Virus and Clinical Phenomes in Nasopharyngeal Carcinoma Patients.

Front Microbiol 2022 30;13:919496. Epub 2022 Jun 30.

Guangxi Health Commission Key Laboratory of Molecular Epidemiology of Nasopharyngeal Carcinoma, Wuzhou Red Cross Hospital, Wuzhou, China.

Plasma lipidomics has been commonly used for biomarker discovery. Studies in cancer have suggested a significant alteration of circulating metabolite profiles which is correlated with cancer characteristics and treatment outcome. However, the lipidomics characteristics of nasopharyngeal carcinoma (NPC) have rarely been studied. We previously described the phenomenon of lipid droplet accumulation in NPC cells and showed that such accumulation could be regulated by latent infection of Epstein-Barr virus (EBV). Here, we compared the plasma lipidome of NPC patients to that of healthy controls by liquid chromatography-tandem mass spectrometry (LC-MS/MS). We found 19 lipids (e.g., phosphatidylinositols 18:0/20:4 and 18:0/18:2 and free fatty acid 22:6) to be remarkably decreased, whereas 2 lipids (i.e., diacylglycerols 16:0/16:1 and 16:0/20:3) to be increased, in the plasma of NPC patients, compared with controls. Different lipid profiles were also observed between patients with different titers of EBV antibodies (e.g., EA-IgA and VCA-IgA) as well as between patients with and without lymph node or distant organ metastasis. In conclusion, plasma lipidomics might help to differentiate NPC cases from controls, whereas EBV infection might influence the risk and prognosis of NPC through modulating lipid metabolism in both tumor cells and peripheral blood.
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http://dx.doi.org/10.3389/fmicb.2022.919496DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9281874PMC
June 2022

Effects of soil colloids on the aggregation and degradation of engineered nanoparticles (TiCT MXene).

Environ Res 2022 Jul 12;214(Pt 1):113886. Epub 2022 Jul 12.

State Environmental Protection Key Laboratory of Synergetic Control and Joint Remediation for Soil & Water Pollution, College of Ecology and Environment, Chengdu University of Technology, Chengdu 610059, China; State Key Laboratory of Geohazard Prevention and Geoenvironment Protection, Chengdu University of Technology, Chengdu 610059, China. Electronic address:

Soil colloid is a nonnegligible factor when evaluating the environmental risk of engineered nanoparticles (ENPs) in the groundwater. In this study, the environmental fate of an emerging ENP (TiCT MXene) in the groundwater was investigated for the first time, which currently poses a severe environmental risk due to its cytotoxicity but has received little attention. The colloidal dispersion stability and degradation kinetics of TiCT MXene in the groundwater were evaluated by considering the effects of soil colloids prepared from sodium humate (SH), montmorillonite (MT), and a natural soil (NS) under variable solution chemistry. The results showed that the affinity of soil colloids with TiCT followed an SH > MT > NS sequence. Increasing SH concentration led to TiCT disaggregation by enhancing the electrical and steric repulsive forces, while MT and NS resulted in hetero-aggregation because of the elevated collision frequency. SH and MT enhanced the critical coagulation concentrations of TiCT by 100 and 10 folders, respectively, via surface coating process, while NS slightly reduced due to the bridging effects induced by the soluble cations. The soil colloids promoted TiCT degradation compared with their absence and in an SH > MT ≫ NS sequence. SH and MT were through forming Ti-O-C and Si-O-Ti bonds with TiCT via their carboxyl and hydroxyl groups, respectively, rendering the TiCT surface more reactive and faster degradation. NS showed a weak promotion effect because of its less affinity with TiCT and limited organic matter and clay contents with hydroxyl and carboxyl groups. This study demonstrated the unstable environmental behaviors of TiCT in the groundwater and mitigated its environmental risk concerns.
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http://dx.doi.org/10.1016/j.envres.2022.113886DOI Listing
July 2022

Efficacy and Safety of Electroacupuncture for Pain Control in Herpes Zoster: A Systematic Review and Meta-Analysis.

Evid Based Complement Alternat Med 2022 4;2022:4478444. Epub 2022 Jul 4.

Department of Acupuncture and Moxibustion, The Third Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China.

Introduction: Herpes zoster is caused by the reactivation of the latent varicella-zoster virus, which leads to acute pain that may disturb routine activities and affect patients' quality of life. Electroacupuncture (EA) has been commonly used for treating herpetic pain in clinical treatment. However, no relevant studies have been performed to evaluate the efficacy and safety of EA for acute control in herpetic neuralgia patients. The purpose of the current study was to conduct a systematic review and meta-analysis to address the deficiencies of the current research.

Methods: Three English (PubMed, Cochrane Library, and Web of Science) and four Chinese (China National Knowledge Infrastructure (CNKI), Chinese Biomedical Literature database (CBM), Wan-fang database, and the Chinese Scientific Journals Full-text Database (VIP)) were comprehensively searched from inception to 31 December 2021. Two independent reviewers evaluated the retrieved data based on the eligibility criteria in advance. In addition, the Cochrane Risk of Bias Tool was used to assess the methodological quality of the included studies. Outcome indexes in this study included the visual analog scale, the time to cessation of pustules, the time to scabs, the time to rash healing, adverse reactions, and the incidence of postherpetic neuralgia. Sensitivity and subgroup analyses were also performed to evaluate the intervention effect specifically. In addition, publication bias was analyzed.

Results: Six randomized controlled trials (167 participants in the experimental groups and 174 participants in the control groups) were identified as reporting the application of EA for acute herpes zoster pain and were included in this study. The results from our meta-analysis revealed that EA was superior to control treatment according to visual analog scale, the time of rash healing, and the incidence of postherpetic neuralgia. However, in terms of the time to cessation of pustules, scabs, and adverse reactions, the results showed that EA compared with the control group showed no significant difference. In addition, subgroup analyses indicated that 2/100 Hz-EA has more significant effects on herpetic pain. Sensitivity analyses revealed that the results of EA for acute pain control and the rash healing time in herpetic neuralgia patients were stable. However, a publication bias was observed.

Conclusion: Our meta-analysis results showed that EA could offer certain advantages in treating acute pain in herpetic neuralgia patients. However, small sample sizes, heterogeneity in study design, and variable methodological quality weaken these inferences. In addition, weak evidence was found for the safety of EA.
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http://dx.doi.org/10.1155/2022/4478444DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273388PMC
July 2022

Overexpression of KITLG predicts unfavorable clinical outcomes and promotes lymph node metastasis via the JAK/STAT pathway in nasopharyngeal carcinoma.

Lab Invest 2022 Jul 13. Epub 2022 Jul 13.

Affiliated Hospital of Guizhou Medical University, Guiyang, China.

Lymph node metastasis (LNM) is an early clinical sign and a contributor to the treatment failure in patients with nasopharyngeal carcinoma (NPC). The molecular mechanisms of LNM in NPC remain unclear. We aimed to identify and validate the possible key genes that play a crucial role in the LNM of NPC. The study included a discovery and validation phase. In the discovery phase, the key gene was identified by bioinformatics analysis. In the validation phase, the mRNA and protein expression of the key gene was detected by RT-PCR in NPC cells and by immunohistochemistry in a tissue microarray. Then, the effect of the key gene expression on cell invasion and migration was explored in vitro and in vivo. As a result, KITLG was identified as the key gene. The overexpression of KITLG was detected in NPC cells, which was correlated with neck lymph node metastasis and poor prognosis in patients with NPC. The suppression of KITLG inhibited the proliferation, invasion, and metastasis of NPC cells in vitro and in vivo. JAK/STAT signaling pathway might mediate the enhancement of cell invasion and metastasis caused by KITLG. In summary, the overexpression of KITLG in NPC cells might play a crucial role in the LNM of NPC, raising the possibility of KITLG as a prognostic factor and a potential target for NPC treatment.
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http://dx.doi.org/10.1038/s41374-022-00817-4DOI Listing
July 2022

Clinical Outcomes of Patients With Unresectable Primary Liver Cancer Treated With Yttrium-90 Radioembolization With an Escalated Dose.

Adv Radiat Oncol 2022 Jul-Aug;7(4):100948. Epub 2022 Mar 21.

Department of Radiation Oncology, Washington University School of Medicine, St. Louis, Missouri.

Purpose: Yttrium-90 (90Y) radioembolization with an escalated dose has been shown to improve clinical outcomes compared with standard dose radioembolization, but there are few data on the local control of primary liver tumors. We reported the clinical outcomes of patients with unresectable primary liver tumors treated with 90Y radioembolization with an escalated dose.

Methods And Materials: Clinical data of patients with unresectable hepatocellular carcinoma (HCC), cholangiocarcinoma (CC), and biphenotypic tumors (cHCC-CC) treated with radioembolization with an escalated dose (≥150 Gy) between 2013 and 2020 with >3 months follow-up were retrospectively reviewed. The primary endpoint was freedom from local progression. Clinical response was defined by Modified Response Evaluation Criteria in Solid Tumours and toxic effects were assessed using Common Terminology Criteria for Adverse Events version 5.0.

Results: Fifty-three patients with HCC and 15 patients with CC/cHCC-CC were analyzed. The median dose delivered was 205 Gy (interquartile range, 183-253 Gy) and 198 Gy (interquartile range, 154-234 Gy) for patients with HCC and CC/cHCC-CC, respectively. The 1-year freedom from local progression rate was 54% (95% confidence interval [CI], 38%-78%) for patients with HCC and 66% (95% CI, 42%-100%) for patients with CC/cHCC-CC. For patients with HCC, United Network for Organ Sharing nodal stage 1 ( = .01), nonsolitary tumors ( = .02), pretreatment α-fetoprotein of >7.7 ng/mL ( = .006), and ≤268 Gy dose delivered ( = .003) were predictors for local progression on multivariate Cox analysis. No patients with HCC who received a dose >268 Gy had a local tumor progression. The 1-year overall survival for patients with HCC was 74% (95% CI, 61%-89%). After radioembolization, 5 (7%) patients had grade 3 ascites, and 4 (6%) patients had grade 3/4 hyperbilirubinemia.

Conclusions: Treatment of unresectable primary liver tumors with 90Y radioembolization with an escalated dose was safe and well tolerated. Delivery of >268 Gy may improve local tumor control of HCC. Determination of the maximum tolerated dose needs to be performed in the context of future prospective dose-escalation trials to further evaluate the safety and efficacy of such an approach.
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http://dx.doi.org/10.1016/j.adro.2022.100948DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9260102PMC
March 2022

Evaluation and comparison of three virucidal agents on inactivation of Nipah virus.

Sci Rep 2022 Jul 5;12(1):11365. Epub 2022 Jul 5.

National Biosafety Laboratory, Chinese Academy of Sciences, Wuhan, 430020, Hubei, People's Republic of China.

Modern human activity is profoundly changing our relationship with microorganisms with the startling rise in the rate of emerging infectious diseases. Nipah virus together with Ebola virus and SARS-CoV-2 are prominent examples. Since COVID-19 and the West African Ebola virus disease outbreak, different chemical disinfectants have been developed for preventing the direct spread of viruses and their efficacy has also been evaluated. However, there are currently no published efficacy studies for the chemical disinfection of Nipah virus. In this study, the virucidal efficacy of three disinfectants (Micro-Chem Plus detergent disinfectant cleaner, FWD and Medical EtOH) against Nipah virus was evaluated in quantitative suspension tests including. Our results showed that the > 4 log reduction achieved for all products in inactivating Nipah virus in 15 s. Even, 19% ethanol was able to inactivate Nipah virus when applied for at least 8 min contact time. Comparative analysis displayed virucidal efficacy of each of the evaluated disinfectants against SARS-CoV-2, Ebola virus and Nipah virus, with only minor differences in working concentrations and contact times required for complete inactivation. We expect that our study can assist in decontamination in healthcare settings and high level biosafety laboratories and can be beneficial to control for emerging enveloped viruses.
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http://dx.doi.org/10.1038/s41598-022-15228-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9255448PMC
July 2022

Prognostic Value of Tumor Size in Patients with Upper Tract Urothelial Carcinoma: A Systematic Review and Meta-analysis.

Eur Urol Open Sci 2022 Aug 28;42:19-29. Epub 2022 Jun 28.

Department of Urology, Peking University Third Hospital, Beijing, China.

Context: The role of tumor size in predicting prognosis in upper tract urothelial carcinoma (UTUC) patients remains poorly defined.

Objective: To assess the prognostic value of tumor size in patients with UTUC through a systematic review and meta-analysis.

Evidence Acquisition: A comprehensive literature search of the PubMed and Embase databases were performed to identify all relevant articles published up to December 2021 according to the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) statement. Available hazard ratios (HRs) and corresponding 95% confidence intervals (95% CIs) were analyzed to evaluate the association between tumor size and survival outcomes.

Evidence Synthesis: A total of 35 articles representing 32 292 patients met the eligibility criteria and were finally included for the meta-analysis. Tumor size was significantly associated with poor outcomes in terms of overall survival (HR = 1.42, 95% CI = 1.28-1.58), cancer-specific survival (HR = 1.66, 95% CI = 1.47-1.88), recurrence-free survival (HR = 1.25, 95% CI = 1.13-1.38), and intravesical recurrence (HR = 1.12, 95% CI = 1.04-1.20). There was between-study heterogeneity in the effect of tumor size on all these meta-analyses, with  < 0.10 and I generally >50%. Subgroup analyses illustrated that the association of tumor size with adverse prognosis in UTUC patients is not affected by treatment modalities. Segmental resection of ureter, whether receiving lymph node dissection, cutoff of tumor size, and region of population were potential sources of heterogeneity. The funnel plot test indicated no significant publication bias in the meta-analysis of survival outcomes.

Conclusions: This study shows that larger tumor size is associated with an increased risk of overall and cancer-specific mortality, and disease recurrence in UTUC. Integration of tumor size with other prognostic indicators may help in risk stratification and individualized treatment of UTUC.

Patient Summary: Through a systematic review and meta-analysis, this study found that larger tumor size is associated with an increased risk of overall and cancer-specific mortality, and disease recurrence in patients with upper tract urothelial carcinoma.
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http://dx.doi.org/10.1016/j.euros.2022.06.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9244730PMC
August 2022

Identification of pyroptosis-related long non-coding RNAs with prognosis and therapy in lung squamous cell carcinoma.

Sci Rep 2022 Jul 1;12(1):11206. Epub 2022 Jul 1.

Shengli Clinical Medical College of Fujian Medical University, Fuzhou, 350001, Fujian, People's Republic of China.

Pyroptosis is a type of programmed cell death with an intense inflammatory response. Previous studies have shown that pyroptosis plays an important role in the pathogenesis and progression of lung cancer. However, the prognostic value and drug targets of pyroptosis-related lncRNAs in lung squamous cell carcinoma (LSCC) have never been studied. In the present study, we identified 1468 pyroptosis-related lncRNAs in LSCC by performing Pearson correlation analysis between the pyroptosis-related genes and the lncRNAs from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) database. The whole set was divided into a training and a test set with a 1:1 ratio. Univariate Cox regression and least absolute shrinkage and selection operator (LASSO) analyses were conducted to establish an 11 multilncRNA signature in the three sets. The signature divided LSCC patients into the low-risk and the high-risk groups. Kaplan-Meier analysis and receiver operating characteristic (ROC) indicated that the prognostic signature had a promising predictive capability for LSCC patients. Besides, the association of microenvironment and immunotherapy response with signature was also analyzed. Moreover, 28 potential compounds targeting signature were screened as possible drugs to treat LSCC. Finally, a nomogram model was constructed to offer the quantitative prediction and net benefit for the prognosis of LSCC patients. In conclusion, the 11 pyroptosis-related lncRNAs and their signature may be promising prognostic factors and therapeutic targets for patients with LSCC.
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http://dx.doi.org/10.1038/s41598-022-15373-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9249737PMC
July 2022

Event-level prediction of urban crime reveals a signature of enforcement bias in US cities.

Nat Hum Behav 2022 Jun 30. Epub 2022 Jun 30.

Department of Medicine, University of Chicago, Chicago, IL, USA.

Policing efforts to thwart crime typically rely on criminal infraction reports, which implicitly manifest a complex relationship between crime, policing and society. As a result, crime prediction and predictive policing have stirred controversy, with the latest artificial intelligence-based algorithms producing limited insight into the social system of crime. Here we show that, while predictive models may enhance state power through criminal surveillance, they also enable surveillance of the state by tracing systemic biases in crime enforcement. We introduce a stochastic inference algorithm that forecasts crime by learning spatio-temporal dependencies from event reports, with a mean area under the receiver operating characteristic curve of ~90% in Chicago for crimes predicted per week within ~1,000 ft. Such predictions enable us to study perturbations of crime patterns that suggest that the response to increased crime is biased by neighbourhood socio-economic status, draining policy resources from socio-economically disadvantaged areas, as demonstrated in eight major US cities.
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http://dx.doi.org/10.1038/s41562-022-01372-0DOI Listing
June 2022

Effect of Apatinib Plus Pegylated Liposomal Doxorubicin vs Pegylated Liposomal Doxorubicin Alone on Platinum-Resistant Recurrent Ovarian Cancer: The APPROVE Randomized Clinical Trial.

JAMA Oncol 2022 Jun 30. Epub 2022 Jun 30.

Department of Gynecologic Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Importance: There are substantial unmet therapeutic needs in patients with platinum-resistant recurrent ovarian cancer (PROC), and novel therapeutic strategies should be explored.

Objective: To evaluate the efficacy and safety of treatment with apatinib (a vascular endothelial growth factor receptor 2 tyrosine kinase inhibitor) plus pegylated liposomal doxorubicin (PLD) for PROC.

Design, Setting, And Participants: The APPROVE trial was performed as an open-label, randomized clinical trial at 11 hospitals in China between March 22, 2018, and November 16, 2020. Patients with histologically confirmed ovarian cancer who had experienced disease progression during or within 6 months of discontinuing any prior line of treatment with platinum-based chemotherapy were eligible. This primary analysis was based on data that were current as of January 28, 2021.

Interventions: Patients received PLD alone (40 mg/m2, intravenously, every 4 weeks, for up to 6 cycles) or PLD plus apatinib (250 mg, orally, daily).

Main Outcomes And Measures: The primary end point was progression-free survival (PFS) by Response Evaluation Criteria in Solid Tumours (RECIST), version 1.1, in the intent-to-treat population.

Results: In total, 152 female patients were randomized, with 78 (51.3%) in the apatinib plus PLD group (median age, 54 years; range, 22-76 years) and 74 (48.7%) in the PLD group (median age, 56 years; range, 33-72 years). The median follow-up duration was 8.7 months (IQR, 4.7-14.1 months). The median PFS was 5.8 months (95% CI, 3.8-8.8) for treatment with apatinib plus PLD vs 3.3 months (95% CI, 2.1-3.8) for PLD (hazard ratio, 0.44; 95% CI, 0.28-0.71; P < .001). The median overall survival was 23.0 months (95% CI, 18.9 to not reached) with treatment with apatinib plus PLD vs 14.4 months (95% CI, 12.1-23.4) with PLD (hazard ratio, 0.66; 95% CI, 0.40-1.09). The most frequent grade 3 or higher treatment-emergent adverse events were decreased neutrophil counts (11 [14.9%] in the apatinib plus PLD group vs 6 [8.3%] in the PLD group), hypertension (6 [8.1%] vs none), and decreased white blood cell count (5 [6.8%] vs 3 [4.2%]). Two patients receiving treatment with apatinib plus PLD experienced grade 2 fistulas.

Conclusions And Relevance: This randomized clinical trial found that treatment with apatinib plus PLD showed promising efficacy and manageable toxic effects in patients with PROC and may be a new alternative treatment option in this setting.

Trial Registration: Clinicaltrials.gov Identifier: NCT04348032.
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http://dx.doi.org/10.1001/jamaoncol.2022.2253DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9247861PMC
June 2022

Current understanding and future perspectives on the extraction, structures, and regulation of muscle function of tea pigments.

Crit Rev Food Sci Nutr 2022 Jun 30:1-23. Epub 2022 Jun 30.

School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, People's Republic of China.

With the aggravating aging of modern society, the sarcopenia-based aging syndrome poses a serious potential threat to the health of the elderly. Natural dietary supplements show great potential to reduce muscle wasting and enhance muscle performance. Tea has been widely recognized for its health-promoting effects. which contains active ingredients such as tea polyphenols, tea pigments, tea polysaccharides, theanine, caffeine, and vitamins. In different tea production processes, the oxidative condensation and microbial transformation of catechins and other natural substances from tea promotes the production of various tea pigments, including theaflavins (TFs), thearubigins (TRs), and theabrownins (TBs). Tea pigments have shown a positive effect on maintaining muscle health. Nevertheless, the relationship between tea pigments and skeletal muscle function has not been comprehensively elucidated. In addition, the numerous research on the extraction and purification of tea pigments is disordered with the limited recent progress due to the complexity of species and molecular structure. In this review, we sort out the strategies for the separation of tea pigments, and discuss the structures of tea pigments. On this basis, the regulation mechanisms of tea pigments on muscle functional were emphasized. This review highlights the current understanding on the extraction methods, molecular structures and regulation mechanisms of muscle function of tea pigments. Furthermore, main limitations and future perspectives are proposed to provide new insights into broadening theoretical research and industrial applications of tea pigments in the future.
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http://dx.doi.org/10.1080/10408398.2022.2093327DOI Listing
June 2022

Mutant D620N and VPS35 induces motor dysfunction and impairs DAT-mediated dopamine recycling pathway.

Hum Mol Genet 2022 Jun 29. Epub 2022 Jun 29.

Department of Neurology, The First Affiliated Hospital, Sun Yat-sen University; Guangdong Provincial Key Laboratory of Diagnosis and Treatment of Major Neurological Diseases; National Key Clinical Department and Key Discipline of Neurology, No.58 Zhongshan Road 2, Guangzhou, 510080, China.

The D620N mutation in vacuolar protein sorting protein 35 (VPS35) gene has been identified to be linked to late onset familial Parkinson disease (PD). However, the pathophysiological roles of VPS35-D620N in PD remain unclear. Here, we generated the transgenic C. elegans overexpressing either human wild type or PD-linked mutant VPS35-D620N in neurons. C. elegans expressing VPS35-D620N, compared with non-transgenic controls, showed movement disorders and dopaminergic neuron loss. VPS35-D620N worms displayed more swimming induced paralysis but showed no defects in BSR assays, thus indicating the disruption of dopamine (DA) recycling back inside neurons. Moreover, VPS35 formed a protein interaction complex with DA transporter (DAT), RAB5, RAB11, and FAM21. In contrast, the VPS35-D620N mutant destabilized these interactions, thus disrupting DAT transport from early endosomes to recycling endosomes, and decreasing DAT at the cell suffice. These effects together increased DA in synaptic clefts, and led to dopaminergic neuron degeneration and motor dysfunction. Treatment with reserpine significantly decreased the swimming induced paralysis in VPS35-D620N worms, as compared with vehicle treated VPS35-D620N worms. Our studies not only provide novel insight into the mechanisms of VPS35-D620N-induced dopaminergic neuron degeneration and motor dysfunction via disruption of DAT function and the DA signaling pathway, but also indicate a potential strategy to treat VPS35-D620N related PD and other disorders.
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http://dx.doi.org/10.1093/hmg/ddac142DOI Listing
June 2022

Automated identification and quantification of invisible microplastics in agricultural soils.

Sci Total Environ 2022 Jun 22;844:156853. Epub 2022 Jun 22.

State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China; Marine Institute for Bioresources and Environment, Peking University Shenzhen Institute, Shenzhen, Guangdong 518057, China. Electronic address:

Microplastics in agricultural soils have become the research hotspot in recent years, however, the quantitative methods based on the traditional visual inspection may have a high false detection rate. Here we combined the laser direct infrared (LDIR) and Fourier-transform infrared (FTIR) methods to investigate the microplastics in farmland with long-term agricultural activities. The results showed that the total abundance of microplastics reached 1.98 ± 0.41 × 10, 1.57 ± 0.28 × 10, 1.78 ± 0.27 × 10, and 3.20 ± 0.41 × 10 particles/kg soil in cotton fields with film mulching of 5, 10, 20, and >30 years, respectively. LDIR results indicated that microplastics ranging from 10 to 500 μm accounted for 96.5-99.9 % of the total microplastic amounts in the soils. Additionally, a total of 26 polymer types of microplastics were detected, among which polyethylene (PE), polypropylene (PP), polyvinyl chloride (PVC), polyamide (PA), and polytetrafluoroethylene (PTFE) were dominantly observed. For the microplastics detected by FTIR (500 μm-5 mm), PE polymer was majorly observed (88.0-98.9 %). Most microplastics were films (88.2 %), while fibers and pellets were also found. The reclaimed water from sewage treatment plants, the drip irrigation utilities, and the residual plastic film are the potential sources of microplastics in the farmland soils. By using the automated quantitative and identifiable approaches, this study suggested that the commonly used visual counting method may underestimate the microplastic contamination in agricultural soils.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156853DOI Listing
June 2022

In Vitro and In Vivo Characterization of a New Strain of Mosquito Flavivirus Derived from .

Viruses 2022 06 14;14(6). Epub 2022 Jun 14.

Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.

Mosquito-specific flaviviruses comprise a group of insect-specific viruses with a single positive RNA, which can affect the duplication of mosquito-borne viruses and the life growth of mosquitoes, and which have the potential to be developed as a vaccine platform for mosquito-borne viruses. In this study, a strain of mosquito flavivirus (MFV) YN15-283-02 was detected in collected from Yunnan, China. The isolation of the purified MFV YN15-283-02 from cell culture failed, and the virus was then rescued by an infectious clone. To study the biological features of MFV YN15-283-02 in vitro and in vivo, electron microscopy, phylogenetic tree, and viral growth kinetic analyses were performed in both cell lines and mosquitoes. The rescued MFV (rMFV) YN15-283-02 duplicated and reached a peak in C6/36 cells at 6 d.p.i. with approximately 2 × 10 RNA copies/μL (RNA to cell ratio of 0.1), but without displaying a cytopathic effect. In addition, the infection rate for the rMFV in show a low level in both larvae (≤15%) and adult mosquitoes (≤12%).
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http://dx.doi.org/10.3390/v14061298DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9229015PMC
June 2022
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