Publications by authors named "Yi He"

967 Publications

A novel intensive conditioning regimen for allogeneic hematopoietic stem cell transplantation in the treatment of relapsed/refractory acute myeloid leukemia.

Neoplasma 2021 Oct 11. Epub 2021 Oct 11.

Department of Hematology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

We conducted a prospective study to evaluate the efficacy and safety of cladribine, cytarabine, mitoxantrone, and granulocyte colony-stimulating factor (CLAG-M) regimen combined with busulfan and cyclophosphamide (Bucy) as new intensive conditioning before allogeneic hematopoietic stem cell transplantation (allo-HSCT) in the treatment of relapsed/refractory acute myeloid leukemia (AML). 24 patients were enrolled. The median follow-up was 15.2 months (range 1.9-67.0 months). Except for one patient who died before graft infusion, the evaluable 23 patients (96%) achieved complete remission (CR). The two-year overall survival (OS) rate and leukemia-free survival (LFS) rate were 61.4% and 59.4%, respectively. The non-relapse mortality (NRM) was 9.1%. Univariate analysis revealed that the myeloid blast phase of chronic myelomonocytic leukemia (CMML), an EVI1 mutated, blood blasts ≥ 20% at transplant and extramedullary disease were risk factors for LFS.
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http://dx.doi.org/10.4149/neo_2021_210426N573DOI Listing
October 2021

Conjugated microporous organic polymer as fluorescent chemosensor for detection of Fe and Fe ions with high selectivity and sensitivity.

Talanta 2022 Jan 14;236:122872. Epub 2021 Sep 14.

College of Chemistry, Jilin University, Changchun, 130012, PR China. Electronic address:

A conjugated microporous organic polymer (TPA-Bp) comprised of triphenylamine (TPA) and 2,2'-bipyridine-5,5'-diformaldehyde (Bp) was prepared via the Schiff-base reaction under ambient conditions. TPA-Bp is an amorphous and microporous spherical nanoparticle with very high stability. TPA-Bp suspension in DMF displayed strong fluorescence emission and selective fluorescence quenching response towards Fe and Fe ions. The fluorescence intensity of TPA-Bp at 331 nm presents linear relationship with the concentrations of both Fe and Fe with low detection limits of 1.02 × 10 M for Fe and 5.37 × 10 M for Fe. The results of X-ray photoelectron spectroscopy (XPS) and Fourier Transform infrared spectroscopy (FTIR) confirm the selective coordination of N atoms of pyridine unit with Fe ions. The fluorescence quenching of TPA-Bp upon the addition of Fe/Fe ions can be attributed to the absorption competition quenching (ACQ) mechanism and the energy transfer between TPA-Bp and Fe/Fe ions. This work demonstrates that the conjugated microporous polymers are promising candidates as luminescent sensor for detection of the special analytes in practical applications.
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http://dx.doi.org/10.1016/j.talanta.2021.122872DOI Listing
January 2022

Leukotriene B4 receptor 2 correlates with prognosis and immune infiltration in clear cell renal cell carcinoma.

Invest New Drugs 2021 Oct 11. Epub 2021 Oct 11.

Department of Oncology, The Affiliated Cancer Hospital of Xiangya School of Medicine, Hunan Cancer Hospital, Central South University, Changsha, Hunan, China.

Background: Clear cell renal cell carcinoma (ccRCC) is the most common renal cancer. According to reports, leukotriene B4 receptor 2 (LTB4R2, also known as BLT2), a chemokine receptor, is upregulated in different tumors. However, the correlation between BLT2 expression and its prognostic value in ccRCC remains to be explored.

Methods: This study used the The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases to evaluate the association between BLT2 expression and the clinical outcome of ccRCC. Based on TIMER2.0, the correlation between BLT2 expression in ccRCC and tumor immune characteristics was evaluated.

Results: The expression of BLT2 in ccRCC was higher than that in normal tissues. Kaplan-Meier survival analysis indicated that high BLT2 expression was significantly correlated with poor overall survival (HR = 1.75, p < 0.001) and disease-specific survival (HR = 1.60, p = 0.014) for patients with ccRCC. In addition, our findings revealed that there was no significant correlation between the M1 marker genes and the expression of BLT2 in ccRCC, while moderate correlations were observed between the BLT2 expression and the M2 marker genes. Tregs and T cell exhaustion marker genes were positively correlated with BLT2 expression in ccRCC (p < 0.001).

Conclusion: BLT2 may serve as a novel prognostic biomarker and is related to the shaping of tumor immune microenvironment in ccRCC. The expression of BLT2 potentially contributes to the regulation of TAMs, T cell exhaustion, and Tregs activation in ccRCC, providing new approaches to promote the development of new immunotherapeutic strategies for ccRCC.
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http://dx.doi.org/10.1007/s10637-021-01174-7DOI Listing
October 2021

Selenomethionine protects oxidative-stress-damaged bone-marrow-derived mesenchymal stem cells via an antioxidant effect and the PTEN/PI3K/AKT pathway.

Exp Cell Res 2021 Oct 6;408(2):112864. Epub 2021 Oct 6.

Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-Sen University, Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, 510055, PR China. Electronic address:

Dental implant surgery is currently a routine therapy for the repair of missing dentition or dentition defects. Both clinical and basic research have elucidated that oxidative stress caused by the accumulation of reactive oxygen species (ROS) for various reasons impairs the process of osteointegration after dental implantation. Therefore, the osteogenic micro-environment must be ameliorated to decrease the damage caused by oxidative stress. Selenomethionine (SEMET) has been reported to play an important role in alleviating oxidative stress and accelerating cell viability and growth. However, it remains unclear whether it exerts protective effects on bone-marrow-derived mesenchymal stem cells (BMSCs) under oxidative stress. In this study, we explored the influence of selenomethionine on the viability and osteogenic differentiation of BMSCs under oxidative stress and the underlying mechanisms. Results showed that 1 μM selenomethionine was the optimum concentration for BMSCs under HO stimulation. HO-induced oxidative stress suppressed the viability and osteogenic differentiation of BMSCs, manifested by the increases in ROS production and cell apoptosis rates, and by the decrease of osteogenic differentiation-related markers. Notably, the aforementioned oxidative damage and osteogenic dysfunction induced by HO were rescued by selenomethionine. Furthermore, we found that the PTEN expression level was suppressed and its downstream PI3K/AKT pathway was activated by selenomethionine. However, when PTEN was stimulated, the PI3K/AKT pathway was down-regulated, and the protective effects of selenomethionine on BMSC osteogenic differentiation diminished, while the inhibition of PTEN up-regulated the protective effects of selenomethionine. Together, these results revealed that selenomethionine could attenuate HO-induced BMSC dysfunction through an antioxidant effect, modulated via the PTEN/PI3K/AKT pathway, suggesting that selenomethionine is a promising antioxidant candidate for reducing oxidative stress during the process of dental implant osteointegration.
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http://dx.doi.org/10.1016/j.yexcr.2021.112864DOI Listing
October 2021

Unsupervised domain adaptation model for lesion detection in retinal OCT images.

Phys Med Biol 2021 Oct 7. Epub 2021 Oct 7.

Jiangsu Key Laboratory of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China, Suzhou, CHINA.

Background and objective Optical Coherence Tomography (OCT) is one of the most used retinal imaging modalities in the clinic as it can provide high-resolution anatomical images. The huge number of OCT images has significantly advanced the development of deep learning methods for automatic lesion detection to ease the doctor's workload. However, it has been frequently revealed that the deep neural network (DNN) model has difficulty handling the domain discrepancies, which widely exist in medical images captured from different devices. Many works have been proposed to solve the domain shift issue in deep learning tasks such as disease classification and lesion segmentation, but few works focused on lesion detection, especially for OCT images. Methods In this work, we proposed a faster-RCNN based, unsupervised domain adaptation model to address the lesion detection task in cross-device retinal OCT images. The domain shift is minimized by reducing the image-level shift and instance-level shift at the same time. We combined a domain classifier with a Wasserstein distance (WD) critic to align the shifts at each level. Results The model was tested on two sets of OCT image data captured from different devices, obtained an average accuracy improvement of more than 8% over the method without domain adaptation, and outperformed other comparable domain adaptation methods. Conclusion The results demonstrate the proposed model is more effective in reducing the domain shift than advanced methods.
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http://dx.doi.org/10.1088/1361-6560/ac2dd1DOI Listing
October 2021

Enhanced Fog Harvesting through Capillary-Assisted Rapid Transport of Droplet Confined in the Given Microchannel.

ACS Appl Mater Interfaces 2021 Oct 5;13(40):48292-48300. Epub 2021 Oct 5.

Key Laboratory of Bioinspired Smart Interfacial Science and Technology of Ministry of Education, School of Chemistry and Beijing Advanced Innovation Center for Biomedical Engineering, Beihang University (BUAA), Beijing 100191, P. R. China.

A novel integrated bioinspired surface is fabricated by using an innovative capillarity-induced selective oxidation method, to achieve the combination of the fog-collecting characteristics of a variety of creatures, i.e., the micronanostructures of spider silk, the wettable patterns of desert beetle, the conical structure of cactus spine, and the hierarchical microchannel of trichome. The fog is captured effectively via multistructures on the cone tips, and captured droplet is collected and confined in the microchannel to realize rapid transport via the formation of wettable pattern on the surface and the introduction of wettable gradient in the microchannel. Consequently, the fog harvest efficiency reaches 2.48 g/h, increasing to nearly 320% compared to the normal surface. More interestingly, similar to trichome, the surface also presents two transport modes, namely, Mode I (water transport along dry microchannel) and Mode II (succeeding water slippage on the water film). In Mode II, the velocity of 34.10 mm/s is about three times faster than that on the trichome. Such a design of integrated bioinspired surface may present potential applications in high-efficiency water collection systems, microfluidic devices, and others.
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http://dx.doi.org/10.1021/acsami.1c14696DOI Listing
October 2021

Effects of the polysaccharides extracted from Chinese yam ( Thunb.) on cancer-related fatigue in mice.

Food Funct 2021 Sep 29. Epub 2021 Sep 29.

Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.

The aim of this study was to investigate the anti-fatigue activity of Chinese Yam polysaccharides (CYPs). The structural characterization of CYPs was conducted using Fourier transform-infrared spectroscopy, nuclear magnetic resonance spectroscopy, gel permeation chromatography-light scattering-refractive index, and ion chromatography. The weight-loaded swimming capability, behavior performance, tumor growth, content of adenosine triphosphate (ATP), and biochemical markers of CYP in a cancer-related fatigue mouse model were tested. The results showed that CYP is a mixture with an average of 75.57 kDa and is mainly composed of rhamnose, glucuronic acid, glucose, galactose, and arabinose with a molar ratio of 0.01 : 0.06 : 1.00 : 0.17 : 0.01. CYP increased the exhausting swimming time, which was decreased in the cisplatin (DDP) control group and the model group. CYP also increased the content of ATP in musculus gastrocnemius, which was down-regulated by DDP; the DDP had significantly enhanced the contents of interleukin-1β (IL-lβ), malondialdehyde (MDA), blood urea nitrogen (BUN) and lactic dehydrogenase (LDH) and inhibited the activity of superoxide dismutase (SOD) in the muscle. Administration of CYP decreased the levels of IL-lβ, MDA, BUN and LDH, and up-regulated the SOD activity. The DDP + CYP group presented a decreased tumor volume and a lower tumor weight as compared with the model group. Moreover, the mice in the CYP or DDP + CYP groups had heavier body weights than the mice in the model group and DDP group. These results suggest that CYP should improve cancer-related fatigue the regulation of inflammatory responses, oxidative stress and increase in energy supplementation.
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http://dx.doi.org/10.1039/d1fo00375eDOI Listing
September 2021

Selenomethionine-Modified Polyethylenimine-Based Nanoparticles Loaded with miR-132-3p Inhibitor-Biofunctionalized Titanium Implants for Improved Osteointegration.

ACS Biomater Sci Eng 2021 10 29;7(10):4933-4945. Epub 2021 Sep 29.

Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-Sen University, Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, PR China.

Titanium and its alloys have been widely used as bone implants, but for reduced treatment span, improvements are urgently needed to achieve faster and better osteointegration. In this study, we found that miR-132-3p inhibited bone-marrow-derived stem cell (BMSC) osteogenic differentiation via targeting BMP2, and that inhibiting miR-132-3p could significantly improve the osteogenic capability of BMSCs. Moreover, we fabricated a biocompatible selenomethionine (SEMET)-modified polyethylene glycol (PEG)/polyethylenimine (PEI) nanoparticle (SeNP) cross-linked with 0.2% gelatin solutions and delivered miR-132-3p inhibitor to biofunctionalize alkali heat-treated titanium implants, resulting in the development of a novel coating for reverse transfection. The biological performances of PEG/PEI/miR-132-3p inhibitor and SeNP/miR-132-3p inhibitor-biofunctionalized titanium were compared. The biological effects, including cell viability, cytotoxicity, adhesion, cellular uptake, and osteogenic capacity of SeNP/miR-132-3p inhibitor-biofunctionalized titanium implants, were then assessed. Results showed that SeNPs presented appropriate morphology, diameter, and positive zeta potential for efficient gene delivery. The transfection efficiency of the SeNP/miR-132-3p inhibitor was comparable to that of the PEG/PEI/miR-132-3p inhibitor, but the former induced less reactive oxygen species (ROS) production and lower apoptosis rates. Confocal laser scanning microscopy (CLSM) demonstrated that SeNP/miR-132-3p inhibitor nanoparticles released from the titanium surfaces and were taken up by adherent BMSCs. In addition, the release profile showed that transfection could obtain a long-lasting silencing effect for more than 2 weeks. The cell viability, cytotoxicity, and cell spreading of SeNP/miRNA-132-3p inhibitor-biofunctionalized titanium were comparable with those of untreated titanium and the SeNP/miRNA-132-3p inhibitor negative control (NC)-biofunctionalized titanium but resulted in higher ALP activity and osteogenic gene expression levels. In vivo animal studies further certified that SeNP/miRNA-132-3p inhibitor nanoparticles from titanium surfaces promoted osteointegration, which was revealed by microcomputed tomography (micro-CT) and histological observations. Taken together, these findings suggested that selenomethionine-modified PEI-based nanoparticles could achieve better biocompatibility. Moreover, titanium implants biofunctionalized by SeNP/miRNA-132-3p inhibitor nanoparticles might have significant clinical potential for more effective osteointegration.
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http://dx.doi.org/10.1021/acsbiomaterials.1c00880DOI Listing
October 2021

β-caryophyllene, an FDA-Approved Food Additive, Inhibits Methamphetamine-Taking and Methamphetamine-Seeking Behaviors Possibly CB2 and Non-CB2 Receptor Mechanisms.

Front Pharmacol 2021 9;12:722476. Epub 2021 Sep 9.

Molecular Targets and Medications Discovery Branch, National Institute on Drug Abuse, Intramural Research Program, Baltimore, MD, United States.

Recent research indicates that brain cannabinoid CB2 receptors are involved in drug reward and addiction. However, it is unclear whether β-caryophyllene (BCP), a natural product with a CB2 receptor agonist profile, has therapeutic effects on methamphetamine (METH) abuse and dependence. In this study, we used animal models of self-administration, electrical brain-stimulation reward (BSR) and microdialysis to explore the effects of BCP on METH-taking and METH-seeking behavior. We found that systemic administration of BCP dose-dependently inhibited METH self-administration under both fixed-ratio and progressive-ratio reinforcement schedules in rats, indicating that BCP reduces METH reward, METH intake, and incentive motivation to seek and take METH. The attenuating effects of BCP were partially blocked by AM 630, a selective CB2 receptor antagonist. Genetic deletion of CB2 receptors in CB2-knockout (CB2-KO) mice also blocked low dose BCP-induced reduction in METH self-administration, suggesting possible involvement of a CB2 receptor mechanism. However, at high doses, BCP produced a reduction in METH self-administration in CB2-KO mice in a manner similar as in WT mice, suggesting that non-CB2 receptor mechanisms underlie high dose BCP-produced effects. In addition, BCP dose-dependently attenuated METH-enhanced electrical BSR and inhibited METH-primed and cue-induced reinstatement of drug-seeking in rats. microdialysis assays indicated that BCP alone did not produce a significant reduction in extracellular dopamine (DA) in the nucleus accumbens (NAc), while BCP pretreatment significantly reduced METH-induced increases in extracellular NAc DA in a dose-dependent manner, suggesting a DA-dependent mechanism involved in BCP action. Together, the present findings suggest that BCP might be a promising therapeutic candidate for the treatment of METH use disorder.
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http://dx.doi.org/10.3389/fphar.2021.722476DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8458938PMC
September 2021

Surface/sub-surface crack-scattered nonlinear rayleigh waves: A full analytical solution based on elastodynamic reciprocity theorem.

Ultrasonics 2022 Jan 14;118:106578. Epub 2021 Sep 14.

Department of Mechanical Engineering, The Hong Kong Polytechnic University, Kowloon, Hong Kong Special Administrative Region; The Hong Kong Polytechnic University Shenzhen Research Institute, Shenzhen 518057, PR China. Electronic address:

High-order harmonics and sub-harmonics that are engendered upon interaction between surface Rayleigh waves and material flaws have been exploited intensively, for characterizing material defects on or near to waveguide surfaces. Nevertheless, theoretical interpretation on underlying physics of defect-induced nonlinear features of Rayleigh waves remains a daunting task, owing to the difficulty in analytically modeling the stress and displacement fields of a Rayleigh wave in the vicinity of defect, in an explicit and accurate manner. In this study, the Rayleigh wave scattered by a surface or a sub-surface micro-crack is scrutinized analytically, and the second harmonic triggered by the clapping and rubbing behaviors of the micro-crack is investigated, based on the elastodynamic reciprocity theorem. With a virtual wave approach, a full analytical, explicit solution to the micro-crack-induced second harmonic wavefield in the propagating Rayleigh wave is ascertained. Proof-of-concept numerical simulation is performed to verify the analytical solution. Quantitative agreement between analytical and numerical results has demonstrated the accuracy of the solution when used to depict a surface/sub-surface crack-perturbed Rayleigh wavefield and to calibrate the crack-induced wave nonlinearity. The analytical modeling and solution advance the use of Rayleigh waves for early awareness and quantitative characterization of embryonic material defects that are on or near to structural surfaces.
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http://dx.doi.org/10.1016/j.ultras.2021.106578DOI Listing
January 2022

Visual colorimetric detection of triacetone triperoxide based on a Fe(II)-promoted thermal decomposition process.

Analyst 2021 Oct 11;146(20):6187-6192. Epub 2021 Oct 11.

National Collaborative Innovation Center for Nuclear Waste and Environmental Safety, School of National Defence Science & Technology, Southwest University of Science and Technology, Mianyang 621010, P. R. China.

Triacetone triperoxide (TATP) explosives, a popular choice for terrorists, have been used in many violent terrorist attacks all over the world. However, simple, rapid, and on-site detection methods of TATP are still lacking. Herein, we present a visual colorimetric method for on-site and rapid detection of TATP based on a Fe(II)-promoted thermal decomposition process of TATP. We discovered that TATP can be decomposed into HO under heating conditions, and it reacts with Fe to produce hydroxyl radicals (˙OH) and Fe the Fenton reaction. The resulting ˙OH and Fe further oxidize colorless 3,3',5,5'-tetramethylbenzidine (TMB) to a yellow oxidized product (oxTMB). These reaction processes remarkably promote the chemical equilibrium shift and decrease the activation energy. Using the TATP-Fe-TMB ternary chromogenic system, the present colorimetric assay for TATP shows a dynamic range of 0.5-30 μM with a low detection limit of 0.12 μM. Additionally, common substances (, inorganic salts, small organic substances, and polymers) do not interfere with TATP detection. This assay can be used for analyzing TATP in real water and camouflage samples. Furthermore, a test-paper-based method was also successfully developed for visual, rapid and on-site detection of TATP.
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http://dx.doi.org/10.1039/d1an01480cDOI Listing
October 2021

Association Between ZNF804A Gene rs1344706 Polymorphism and Brain Functions in Healthy Individuals: A Systematic Review and Voxel-Based Meta-Analysis.

Neuropsychiatr Dis Treat 2021 14;17:2925-2935. Epub 2021 Sep 14.

Department of Psychology, Chengdu Medical College, Chengdu, 610500, People's Republic of China.

Objective: Zinc finger protein 804A (ZNF804A) protein participates in embryonic neural repair and development. The single nucleotide polymorphism rs1344706 in ZNF804A gene is closely related to functional abnormalities of the human brain. However, these results are inconsistent. This association was verified by meta-analysis in this study.

Methods: Fifteen studies on functional magnetic resonance imaging involving 1710 healthy individuals were included in the systematic review and meta-analysis used by Anisotropic Effect-Size Signed Differential Mapping software.

Results: Functional connectivity of the right dorsolateral prefrontal cortex (rDLPFC)-left hippocampus in the rs1344706 risk allele carrier was significantly increased ( = 2.066, < 0.001), while those in the rDLPFC-left middle frontal gyrus ( = -1.420, < 0.001) and rDLPFC-right middle frontal gyrus ( = -1.298, < 0.001) were significantly decreased. Neural activity of the left anterior cingulate gyrus in the rs1344706 risk allele carrier was significantly decreased ( = -2.525, < 0.001). Sensitivity analysis was almost stable, and no publication bias was found.

Conclusion: The changes in brain function have a clear correlation with ZNF804A gene in healthy individuals, which indicate the contribution of genetic variants on brain dysfunction.

Registration Number: This meta-analysis is registered in PROSPERO (No. CRD42016051331).
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http://dx.doi.org/10.2147/NDT.S322114DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8449690PMC
September 2021

EPHB4 Regulates the Proliferation and Metastasis of Oral Squamous Cell Carcinoma through the HMGB1/NF-κB Signalling Pathway.

J Cancer 2021 20;12(20):5999-6011. Epub 2021 Aug 20.

Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University. Guangzhou, Guangdong, China, 510055.

Malignant proliferation and cervical lymphatic metastasis restrict the prognosis of oral squamous cell carcinoma (OSCC). Erythropoietin-producing human hepatocellular B4 (EPHB4) regulates a series of tumour functions involving tumourigenesis, cancer cell attachment and metastasis. However, the mechanism of EphB4 regulating the malignant progression of OSCC has not been fully elucidated. EPHB4 expression was analysed in 65 OSCC samples and adjacent noncancerous tissues through immunohistochemistry (IHC). siRNA and overexpression plasmids were transfected into OSCC cells to modify EPHB4 expression, and then, regulatory functions were explored and . Co-immunoprecipitation (Co-IP) and mass spectrometry were applied to detect proteins interacting with EPHB4. Subsequently, protein stability assays and NF-κB pathway inhibition assays were used to verify the regulation of EPHB4, high-mobility group box 1 (HMGB1) and nuclear factor-κB (NF-κB) activation. EPHB4 was found to be highly expressed in OSCC tissues, which was related to tumour stage and lymphatic metastasis and resulted in a poor prognosis. Cellular experiments and mouse tongue xenograft models further confirmed that high EPHB4 expression promoted the proliferation and metastasis of OSCC tumours. Mechanistically, co-IP and mass spectrometry studies indicated that EPHB4 could bind to HMGB1 and maintain HMGB1 stability. Downregulation of HMGB1 inhibited the proliferation and metastasis of OSCC cells and inhibited NF-κB phosphorylation activation but did not affect EPHB4 expression. This study revealed the mechanism by which EPHB4 promotes the proliferation and metastasis of OSCC by activating the HMGB1-mediated NF-κB signalling pathway, which can be exploited as a novel marker or therapeutic target to control metastasis and improve the survival rate of OSCC.
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http://dx.doi.org/10.7150/jca.59331DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8425198PMC
August 2021

Correction to: Subtraction improves the accuracy of coronary CT angiography for detecting obstructive disease in severely calcified segments.

Eur Radiol 2021 Sep 16. Epub 2021 Sep 16.

Department of Radiology, Beijing Friendship Hospital, Capital Medical University, No. 95 YongAn Road, Beijing, 100050, People's Republic of China.

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http://dx.doi.org/10.1007/s00330-021-08223-yDOI Listing
September 2021

[Corrigendum] Murine and Chinese cobra venom‑derived nerve growth factor stimulate chondrogenic differentiation of BMSCs : A comparative study.

Mol Med Rep 2021 Nov 16;24(5). Epub 2021 Sep 16.

Guangxi Engineering Center in Biomedical Material for Tissue and Organ Regeneration, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.

Subsequently to the publication of this paper, an interested reader drew to the authors' attention that Figs. 2 and 3, showing the results from experiments designed to assess the viability of bone‑derived mesenchymal stem cells culture with or without nerve growth factors (NGFs) via fluorescein diacetate/propidium iodide or H&E staining respectively, contained apparently duplicated data panels within the figures. After having examined their original data, the authors have realized that these figures were inadvertently assembled incorrectly, and that there were also misassembled data panels in Fig. 6, which showed the secretion of types I and II collagens in bone‑derived mesenchymal stem cells with or without NGFs. The corrected versions of Figs. 2, 3 and 6 are shown below and on the next page. Note that these errors did not significantly affect the results or the conclusions reported in this paper, and all the authors agree to this Corrigendum. Furthermore, the authors apologize to the readership for any inconvenience caused. [the original article was published in 10.3892/mmr.2018.9307].
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http://dx.doi.org/10.3892/mmr.2021.12444DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8477180PMC
November 2021

Erratum: Weakly supervised anomaly segmentation in retinal OCT images using an adversarial learning approach: publisher's note.

Biomed Opt Express 2021 Aug 30;12(8):5337. Epub 2021 Jul 30.

Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Science, 510530 Guangzhou, China.

[This corrects the article on p. 4713 in vol. 12.].
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http://dx.doi.org/10.1364/BOE.438948DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8407815PMC
August 2021

Weakly supervised anomaly segmentation in retinal OCT images using an adversarial learning approach.

Biomed Opt Express 2021 08 7;12(8):4713-4729. Epub 2021 Jul 7.

School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, China.

Lesion detection is a critical component of disease diagnosis, but the manual segmentation of lesions in medical images is time-consuming and experience-demanding. These issues have recently been addressed through deep learning models. However, most of the existing algorithms were developed using supervised training, which requires time-intensive manual labeling and prevents the model from detecting unaware lesions. As such, this study proposes a weakly supervised learning network based on CycleGAN for lesions segmentation in full-width optical coherence tomography (OCT) images. The model was trained to reconstruct underlying normal anatomic structures from abnormal input images, then the lesions can be detected by calculating the difference between the input and output images. A customized network architecture and a multi-scale similarity perceptual reconstruction loss were used to extend the CycleGAN model to transfer between objects exhibiting shape deformations. The proposed technique was validated using an open-source retinal OCT image dataset. Image-level anomaly detection and pixel-level lesion detection results were assessed using area-under-curve (AUC) and the Dice similarity coefficient, producing results of 96.94% and 0.8239, respectively, higher than all comparative methods. The average test time required to generate a single full-width image was 0.039 s, which is shorter than that reported in recent studies. These results indicate that our model can accurately detect and segment retinopathy lesions in real-time, without the need for supervised labeling. And we hope this method will be helpful to accelerate the clinical diagnosis process and reduce the misdiagnosis rate.
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http://dx.doi.org/10.1364/BOE.426803DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8407839PMC
August 2021

Correction to: Occurrence and Prognostic Value of Perineural Invasion in Esophageal Squamous Cell Cancer: A Retrospective Study.

Ann Surg Oncol 2021 Sep 9. Epub 2021 Sep 9.

Department of Thoracic Surgery, Section of Esophageal Surgery, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, China.

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http://dx.doi.org/10.1245/s10434-021-10760-1DOI Listing
September 2021

Chemical composition characteristics and source analysis of PM in Jiaxing, China: Insights into the effect of COVID-19 outbreak.

Environ Technol 2021 Sep 9:1-29. Epub 2021 Sep 9.

College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310000, China.

Jiaxing is a medium-sized city in the Yangtze River Delta (YRD), which showed complex local and surrounding pollution sources. To study the COVID-19 impact on the ambient PM in Jiaxing, we collected the PM samples from January 2 to April 25, 2020 and analyzed their chemical compositions (including carbon components, water-soluble ions (WSIs), and inorganic elements). The concentration of PM was 83.13 30.93 μg/m before COVID-19 pandemic, and then remarkably decreased with COVID-19 outbreak due to the suspension of mobility and industrial activities. Meanwhile, the concentrations of main chemical species (carbon components, water-soluble ions and inorganic elements) of PM all decreased from period A (January 2 to 20, 2020) to period B (January 23 to February 10, 2020). Moreover, Trajectory clustering analysis showed that close-range transport was one of the dominant factors throughout all the period, except for period D (April 1 to 25, 2020). In addition, PSCF model indicated that the COVID-19 outbreak resulted in a significant decrease of WPSCF value. This study highlighted the differences in chemical compositions and sources of PM since COVID-19 pandemic were reported and provide a better understanding of the impact of COVID-19 outbreak on PM.
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http://dx.doi.org/10.1080/09593330.2021.1979104DOI Listing
September 2021

CdGAP promotes prostate cancer metastasis by regulating epithelial-to-mesenchymal transition, cell cycle progression, and apoptosis.

Commun Biol 2021 09 7;4(1):1042. Epub 2021 Sep 7.

Cancer Research Program, Research Institute of the McGill University Health Centre, Montréal, QC, Canada.

High mortality of prostate cancer patients is primarily due to metastasis. Understanding the mechanisms controlling metastatic processes remains essential to develop novel therapies designed to prevent the progression from localized disease to metastasis. CdGAP plays important roles in the control of cell adhesion, migration, and proliferation, which are central to cancer progression. Here we show that elevated CdGAP expression is associated with early biochemical recurrence and bone metastasis in prostate cancer patients. Knockdown of CdGAP in metastatic castration-resistant prostate cancer (CRPC) PC-3 and 22Rv1 cells reduces cell motility, invasion, and proliferation while inducing apoptosis in CdGAP-depleted PC-3 cells. Conversely, overexpression of CdGAP in DU-145, 22Rv1, and LNCaP cells increases cell migration and invasion. Using global gene expression approaches, we found that CdGAP regulates the expression of genes involved in epithelial-to-mesenchymal transition, apoptosis and cell cycle progression. Subcutaneous injection of CdGAP-depleted PC-3 cells into mice shows a delayed tumor initiation and attenuated tumor growth. Orthotopic injection of CdGAP-depleted PC-3 cells reduces distant metastasic burden. Collectively, these findings support a pro-oncogenic role of CdGAP in prostate tumorigenesis and unveil CdGAP as a potential biomarker and target for prostate cancer treatments.
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http://dx.doi.org/10.1038/s42003-021-02520-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8423782PMC
September 2021

Exome-based preconception carrier testing for consanguineous couples in China.

Prenat Diagn 2021 Oct 9;41(11):1425-1429. Epub 2021 Sep 9.

Prenatal Diagnostic Center, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, Guangdong, China.

Objective: To evaluate the utility of clinical exome sequencing (ES)-based carrier screening in Chinese consanguineous couples.

Methods: Consanguineous couples were screened for autosomal recessive (AR) disorders using the clinical ES of 5000 genes associated with human diseases.

Results: We recruited 14 couples who elected to have sequencing. One couple was related as first cousins and 13 as second cousins. Both partners carrying the same pathogenic variant were detected in four couples. One couple was found in which one partner carried a splice variant, and the other had a missence variant of the same gene. These five couples were identified as being at risk of having a child affected by an AR disorder.

Conclusion: Our study demonstrates that ES-based preconception screening yields a clinical value for Chinese consanguineous couples. It enables to detect at-risk couples for rare AR diseases.
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http://dx.doi.org/10.1002/pd.6018DOI Listing
October 2021

Downregulation of Filamin a Expression in the Aorta Is Correlated With Aortic Dissection.

Front Cardiovasc Med 2021 13;8:690846. Epub 2021 Aug 13.

Division of Cardiothoracic and Vascular Surgery, Sino-Swiss Heart-Lung Transplantation Institute, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Filamins (FLNs) are actin cross-linking proteins, and as scaffolding proteins, FLNs are closely associated with the stabilization of the cytoskeleton. Nevertheless, the biological importance of FLNs in aortic dissection (AD) has not been well-elucidated. In this study, we first reanalyzed datasets downloaded from the Gene Expression Omnibus (GEO) database, and we found that in addition to the extracellular matrix, the actin cytoskeleton is a key structure associated with AD. Given that FLNs are involved in remodeling the cytoskeleton to affect cellular functions, we measured their expression levels in the aortas of patients with Stanford type A AD (TAAD). Our results showed that the mRNA and protein levels of FLNA were consistently decreased in dissected aortas of both humans and mice, while the FLNB protein level was upregulated despite decreased FLNB mRNA levels, and comparable expression levels of FLNC were observed between groups. Furthermore, the immunohistochemistry results demonstrated that FLNA was highly expressed in smooth muscle cells (SMCs) of aorta in non-AD samples, and downregulated in the medial layer of the dissected aortas of humans and mice. Moreover, we revealed that FOS and JUN, forming a dimeric transcription factor called AP-1 (activating protein-1), were positively correlated with the expression of FLNA in aorta. Either overexpression of FOS or JUN alone, or overexpression of FOS and JUN together, facilitated the expression of FLNA in primary cultured human aortic SMCs. In the present study, we not only detected the expression pattern of FLNs in aortas of humans and mice with or without AD, but we also found that the expression of FLNA in the AD samples was significantly reduced and that AP-1 might regulate the expression of FLNA. Our findings will contribute to the elucidation of the pathological mechanisms of AD and provide potential therapeutic targets for AD.
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http://dx.doi.org/10.3389/fcvm.2021.690846DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8414519PMC
August 2021

Attenuation of rheumatoid arthritis through the inhibition of caspase3/GSDME-mediated pyroptosis induced by TNF-α.

Arthritis Rheumatol 2021 Sep 4. Epub 2021 Sep 4.

Department of Rheumatology and Immunology, The Third Affiliated Hospital, Southern Medical University, Guangzhou, China.

Objectives: To determine the role of gasdermin E (GSDME)-mediated pyroptosis in the pathogenesis and progression of rheumatoid arthritis (RA), and explore the potential therapeutic targets for RA.

Methods: The expression and activation of caspase3 and GSDME in the synovium, macrophages, and monocytes of RA patients was detected by immunohistochemistry, immunofluorescence, and western blot analysis. The correlation of activated GSDME with RA disease activity was evaluated. The pyroptotic ability of monocytes from RA patients was also tested. In addition, the effect of TNF-α on caspase3/GSDME-mediated pyroptosis of monocytes and macrophages was investigated. Furthermore, mice lacking Gsdme were subjected to collagen-induced arthritis, and the incidence and severity of arthritis was assessed.

Results: Monocytes and synovial macrophages from RA patients showed increased expression of activated caspase3, GSDME, and GSDME-N. The expression of GSDME-N in monocytes from RA patients correlated positively with the disease activity. Monocytes from RA patients with higher GSDME levels were more susceptible to pyroptosis. Furthermore, TNF-α induced pyroptosis in monocytes and macrophages by activating the caspase3/GSDME pathway. The use of a caspase3 inhibitor and silencing of GSDME significantly blocked TNF-α-induced pyroptosis. Gsdme deficiency effectively alleviated arthritis in a collagen-induced arthritis mice model.

Conclusions: These results support a pathogenic role of GSDME in RA and provide an alternative mechanism for RA pathogenesis involving TNF-α, which activates GSDME-mediated pyroptosis of monocytes and macrophages in RA. In addition, targeting GSDME might be a potential therapeutic approach for RA.
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http://dx.doi.org/10.1002/art.41963DOI Listing
September 2021

ASO Visual Abstract: Occurrence and Prognostic Value of Perineural Invasion in Esophageal Squamous Cell Cancer: A Retrospective Study.

Ann Surg Oncol 2021 Sep 3. Epub 2021 Sep 3.

Department of Thoracic Surgery, Section of Esophageal Surgery, Shanghai Chest Hospital, Shanghai Jiao Tong University, 241 Huaihai West Rd., Shanghai, 200030, China.

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http://dx.doi.org/10.1245/s10434-021-10741-4DOI Listing
September 2021

Triage Modeling for Differential Diagnosis Between COVID-19 and Human Influenza A Pneumonia: Classification and Regression Tree Analysis.

Front Med (Lausanne) 2021 10;8:673253. Epub 2021 Aug 10.

Huashan Hospital, Fudan University, Shanghai, China.

The coronavirus disease 2019 (COVID-19) pandemic has lasted much longer than an influenza season, but the main signs, symptoms, and some imaging findings are similar in COVID-19 and influenza patients. The aim of the current study was to construct an accurate and robust model for initial screening and differential diagnosis of COVID-19 and influenza A. All patients in the study were diagnosed at Fuyang No. 2 People's Hospital, and they included 151 with COVID-19 and 155 with influenza A. The patients were randomly assigned to training set or a testing set at a 4:1 ratio. Predictor variables were selected based on importance, assessed by random forest algorithms, and analyzed to develop classification and regression tree models. In the optimal model A, the best single predictor of COVID-19 patients was a normal or high level of low-density lipoprotein cholesterol, followed by low level of creatine kinase, then the presence of <3 respiratory symptoms, then a highest temperature on the first day of admission <38°C. In the suboptimal model B, the best single predictor of COVID-19 was a low eosinophil count, then a normal monocyte ratio, then a normal hematocrit value, then a highest temperature on the first day of admission of <37°C, then a complete lack of respiratory symptoms. The two models provide clinicians with a rapid triage tool. The optimal model can be used to developed countries/regions and major hospitals, and the suboptimal model can be used in underdeveloped regions and small hospitals.
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http://dx.doi.org/10.3389/fmed.2021.673253DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8382719PMC
August 2021

Identification of a circRNA-miRNA-mRNA regulatory network for exploring novel therapeutic options for glioma.

PeerJ 2021 6;9:e11894. Epub 2021 Aug 6.

Neurosurgery Department, Xiangya Hospital Central South University, Changsha, Hunan, China.

Background: Glioma is the most common brain neoplasm with a poor prognosis. Circular RNA (circRNA) and their associated competing endogenous RNA (ceRNA) network play critical roles in the pathogenesis of glioma. However, the alteration of the circRNA-miRNA-mRNA regulatory network and its correlation with glioma therapy haven't been systematically analyzed.

Methods: With GEO, GEPIA2, circBank, CSCD, CircInteractome, mirWalk 2.0, and mirDIP 4.1, we constructed a circRNA-miRNA-mRNA network in glioma. LASSO regression and multivariate Cox regression analysis established a hub mRNA signature to assess the prognosis. GSVA was used to estimate the immune infiltration level. Potential anti-glioma drugs were forecasted using the cMap database and evaluated with GSEA using GEO data.

Results: A ceRNA network of seven circRNAs (hsa_circ_0030788/0034182/0000227/ 0018086/0000229/0036592/0002765), 15 miRNAs(hsa-miR-1200/1205/1248/ 1303/3925-5p/5693/581/586/599/607/640/647/6867-5p/767-3p/935), and 46 mRNAs (including 11 hub genes of ARHGAP11A, DRP2, HNRNPA3, IGFBP5, IP6K2, KLF10, KPNA4, NRP2, PAIP1, RCN1, and SEMA5A) was constructed. Functional enrichment showed they influenced majority of the hallmarks of tumors. Eleven hub genes were proven to be decent prognostic signatures for glioma in both TCGA and CGGA datasets. Forty-six LASSO regression significant genes were closely related to immune infiltration. Finally, five compounds (fulvestrant, tanespimycin, mifepristone, tretinoin, and harman) were predicted as potential treatments for glioma. Among them, mifepristone and tretinoin were proven to inhibit the cell cycle and DNA repair in glioma.

Conclusion: This study highlights the potential pathogenesis of the circRNA-miRNA-mRNA regulatory network and identifies novel therapeutic options for glioma.
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http://dx.doi.org/10.7717/peerj.11894DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8351580PMC
August 2021

Occurrence and Prognostic Value of Perineural Invasion in Esophageal Squamous Cell Cancer: A Retrospective Study.

Ann Surg Oncol 2021 Aug 23. Epub 2021 Aug 23.

Department of Thoracic Surgery, Section of Esophageal Surgery, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, China.

Objective: The aim of this study was to explore the occurrence and prognostic value of perineural invasion (PNI) as a classic tumor pathological feature in esophageal squamous cell carcinoma (ESCC).

Methods: We retrospectively enrolled 794 ESCC patients who underwent radical esophagectomy at Shanghai Chest Hospital from 2017 to 2018. The incidence, associated factors, and prognosis of PNI were analyzed.

Results: PNI was identified in 15.7% (125/794) of patients. The presence of PNI was significantly associated with depth of invasion (p < 0.001), pN stage (p = 0.008), tumor stage (p < 0.001), and lymphovascular invasion (LVI; p < 0.001). Multivariate logistic regression analysis demonstrated that advanced pT stage and LVI were independently associated with the presence of PNI, while multivariate Cox regression analysis demonstrated that PNI was not an independent risk factor for poor overall survival (OS) or recurrence-free survival (RFS) in ESCC patients (OS hazard ratio [HR] 0.688, 95% confidence interval [CI] 0.448-1.056, p = 0.087; RFS HR 0.837, 95% CI 0.551-1.273, p = 0.406). In the PNI-positive patient subgroup, adjuvant therapy was associated with better OS and RFS.

Conclusion: PNI correlates with, and may be a concomitant consequence of, LVI and advanced tumor invasion (T3-4) in ESCC patients. Although PNI was not identified as an independent prognostic indicator, our results suggest ESCC patients with PNI should be considered for adjuvant therapy.
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http://dx.doi.org/10.1245/s10434-021-10665-zDOI Listing
August 2021

Brain-Specific Oxysterols and Risk of Schizophrenia in Clinical High-Risk Subjects and Patients With Schizophrenia.

Front Psychiatry 2021 2;12:711734. Epub 2021 Aug 2.

The National Clinical Research Center for Mental Disorders and Beijing Key Laboratory of Mental Disorders, Beijing Anding Hospital, Capital Medical University, Beijing, China.

Accumulating evidence from clinical, genetic, and epidemiologic studies suggest that schizophrenia might be a neuronal development disorder. While oxysterols are important factors in neurodevelopment, it is unknown whether oxysterols might be involved in development of schizophrenia. The present study investigated the relationship between tissue-specifically originated oxysterols and risk of schizophrenia. A total of 216 individuals were recruited in this study, including 76 schizophrenia patients, 39 clinical high-risk (CHR) subjects, and 101 healthy controls (HC). We investigated the circulating levels of brain-specific oxysterol 24(S)-hydroxycholesterol (24OHC) and peripheral oxysterol 27-hydroxycholesterol (27OHC) in all participants and analyzed the potential links between the oxysterols and specific clinical symptoms in schizophrenic patients and CHR. Our data showed an elevation of 24OHC in both schizophrenia patients and CHR than that in HC, while a lower level of 27OHC in the schizophrenia group only. The ratio of 24OHC to 27OHC was only increased in the schizophrenic group compared with CHR and HC. For the schizophrenic patients, the circulating 24OHC levels are significantly associated with disease duration, positively correlated with the positive and negative syndrome total scores, while the 27OHC levels were inversely correlated with the positive symptom scores. Together, our data demonstrated the disruption of tissue-specifically originated cholesterol metabolism in schizophrenia and CHR, suggesting the circulating 24OHC or 24OHC/27OHC ratio might not only be a potential indicator for risk for schizophrenia but also be biomarkers for functional abnormalities in neuropathology of schizophrenia.
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http://dx.doi.org/10.3389/fpsyt.2021.711734DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8367079PMC
August 2021

The Cancers-Specific Survival of Metastatic Pulmonary Carcinoids and Sites of Distant Metastasis: A Population-Based Study.

Technol Cancer Res Treat 2021 Jan-Dec;20:15330338211036528

Department of Thoracic Surgery, 89657The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.

Background: Lung cancer is the leading cause of cancer-related deaths and pulmonary carcinoids (PCs) account for almost 2% of all pulmonary malignancies. However, few published articles have reported prognosis and related factors of pulmonary carcinoid patients.

Material And Method: The Surveillance, Epidemiology, and End Results (SEER) database was used to collect data of patients diagnosed with metastatic PCs from 2010 to 2016. The prognosis and survival of these patients were compared by employing Cox proportional hazards and the Kaplan-Meier survival analysis.

Results: A total of 1763 patients were analyzed. The liver (668, 25.6%) was shown to be the most common metastatic site in the isolated organ metastasis cohort, followed by the lung (636, 24.4%), bone (562, 21.6%), and brain (460, 17.6%). Among the patients, the tumor metastasized to a single distant site included the liver, bone, lung, and brain. Cancer-specific survival (CSS) in metastatic PCs is determined by the site of metastasis and the total number of such sites. Pulmonary carcinoid patients with isolated liver metastasis manifested more favorable survival rates in comparison to patients having isolated metastasis in the lung, brain, or bone. The median CSS was 45, 7, 6, 5 months ( = 0.011). The number of distant metastatic sites and the location of distant metastasis were found to be independent risk factors for CSS. For patients with distant isolated metastasis, liver metastasis ( < 0.0001) had better CSS in comparison to those with bone metastasis. When compared to patients whose carcinoids had metastasized to the bones, patients with a brain ( = 0.273) or lung ( = 0.483) metastasis had the same CSS.

Conclusion: Cancer-specific survival in metastatic PCs depends on the site of metastasis and the total number of such locations. PC patients with isolated liver metastasis manifested more favorable survival in comparison to patients with isolated metastasis in the lung, brain, or bone.
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http://dx.doi.org/10.1177/15330338211036528DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8361524PMC
August 2021

Linkage and association mapping and Kompetitive allele-specific PCR marker development for improving grain protein content in wheat.

Theor Appl Genet 2021 Nov 10;134(11):3563-3575. Epub 2021 Aug 10.

Provincial Key Lab for Agrobiology, Jiangsu Academy of Agricultural Sciences, 50 Zhongling Street, Nanjing, 210014, Jiangsu, China.

Key Message: Linkage and association mapping identified nine candidate intervals for wheat GPC, and large-scale association mapping based on 9 corresponding KASP markers and 1163 F breeding lines revealed 3 significant markers. Wheat grain protein content (GPC) is an important quality indicator. The GPC of wheat grown in the middle and lower reaches of the Yangtze River is often low. Marker-assisted selection (MAS) is an effective tool for improving quantitative traits; however, most markers have not been effectively applied in GPC improvement except Gpc-B1, although many loci associated with GPC were identified. In this study, linkage analysis using a recombinant inbred line population from the cross of core parents of Ningmai 9 and Yangmai 158 and association mapping using the local cultivated varieties were performed and nine candidate intervals were identified. The appropriate kompetitive allele-specific PCR (KASP) markers associated with GPC were successfully developed and screened in 1163 F4 breeding lines. Three markers, Kgpc-2B, Kgpc-2D, and Kgpc-4A, were validated to be significantly related to GPC by large-scale association mapping, and they were combined to achieve the highest efficiency to enhance GPC. We applied these markers in 164 F6 breeding lines and obtained 15 lines with high GPC, indicating their high selective efficiency. Further, strategies for gene exploration in the three significant intervals were proposed. These results were expected to provide a novel route for improving GPC in wheat quality breeding.
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http://dx.doi.org/10.1007/s00122-021-03913-zDOI Listing
November 2021
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