Publications by authors named "Yi Gao"

966 Publications

Open-source algorithm and software for computed tomography-based virtual pancreatoscopy and other applications.

Vis Comput Ind Biomed Art 2022 Aug 3;5(1):20. Epub 2022 Aug 3.

School of Biomedical Engineering, Health Science Center, Shenzhen University, Shenzhen, 518060, China.

Pancreatoscopy plays a significant role in the diagnosis and treatment of pancreatic diseases. However, the risk of pancreatoscopy is remarkably greater than that of other endoscopic procedures, such as gastroscopy and bronchoscopy, owing to its severe invasiveness. In comparison, virtual pancreatoscopy (VP) has shown notable advantages. However, because of the low resolution of current computed tomography (CT) technology and the small diameter of the pancreatic duct, VP has limited clinical use. In this study, an optimal path algorithm and super-resolution technique are investigated for the development of an open-source software platform for VP based on 3D Slicer. The proposed segmentation of the pancreatic duct from the abdominal CT images reached an average Dice coefficient of 0.85 with a standard deviation of 0.04. Owing to the excellent segmentation performance, a fly-through visualization of both the inside and outside of the duct was successfully reconstructed, thereby demonstrating the feasibility of VP. In addition, a quantitative analysis of the wall thickness and topology of the duct provides more insight into pancreatic diseases than a fly-through visualization. The entire VP system developed in this study is available at https://github.com/gaoyi/VirtualEndoscopy.git .
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http://dx.doi.org/10.1186/s42492-022-00116-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9346031PMC
August 2022

Assessment of Neoadjuvant Treatment Response Using Automated Breast Ultrasound in Breast Cancer.

J Breast Cancer 2022 Jul 8. Epub 2022 Jul 8.

Department of Ultrasound, Xijing Hospital, Fourth Military Medical University, Xi'an, China.

Breast imaging techniques are used to assess the tumor response to neoadjuvant treatment (NAT), which is increasingly one of the preferred therapeutic options and increases the rate of breast conservation for breast cancer. Herein, we report a case in which a woman was diagnosed with invasive ductal carcinoma in the left breast and received NAT before surgery. Automated breast ultrasound (AB US) was regularly performed before and during the NAT to evaluate the tumor response to NAT by measuring diameter changes and volume reductions of the tumor. Images showed that the tumor size was significantly reduced and disappeared after 7 cycles of NAT, except for macrocalcification. Postoperative histopathological examination confirmed that there were no residual tumor cells. We found that AB US overcame the limitations of handheld US, such as operator dependence, poor reproducibility and limited field of view, and can be an alternative modality to assess the tumor response of NAT in the absence of magnetic resonance imaging (MRI) instruments.
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http://dx.doi.org/10.4048/jbc.2022.25.e32DOI Listing
July 2022

Design, synthesis and biological evaluation of novel chromone-maleimide hybrids as potent anti-inflammatory agents against LPS-induced acute lung injury.

Bioorg Chem 2022 Jul 25;128:106049. Epub 2022 Jul 25.

Chemical Biology Research Center at School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China. Electronic address:

Acute lung injury (ALI) is an acute inflammatory disease, which severely impacts lung function with a high lethality rate. Chromone and maleimide are very important moieties of anti-inflammatory agents. Here, forty new chromone-maleimide hybrids were readily synthesized using a Heck-type coupling strategy in good yields and were screened for their anti-inflammatory activity. A majority of these hybrids showed high inhibitory potency against LPS-stimulated release of pro-inflammatory cytokines in macrophages. Preliminary structure-activity relationship studies led to the discovery of highly potent inhibitors. Five of them were found to inhibit lipopolysaccharide (LPS)-induced IL-6 and TNF-α release in a dose-dependent manner with IC values in the nanomolar rang. Furthermore, in vivo administration of 5e and 5g resulted in distinctly attenuated LPS-induced ALI via inhibiting the inflammation. Thus it is evident from our study that these novel chromone-maleimide hybrids present promising therapeutic potential for ALI.
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http://dx.doi.org/10.1016/j.bioorg.2022.106049DOI Listing
July 2022

Calculating PFAS interfacial adsorption as a function of salt concentration using model parameters determined from chemical structure.

Sci Total Environ 2022 Jul 27;848:157663. Epub 2022 Jul 27.

School of Civil and Environmental Engineering, Water Research Centre, University of New South Wales, Sydney, NSW 2052, Australia.

Per- and polyfluoroalkyl substances (PFAS) are widely-detected environmental contaminants known to concentrate at surfaces and interfaces. Many of the most commonly-detected PFAS function as ionic surfactants under environmental conditions. The interfacial behaviors of ionic surfactants, including PFAS, are strongly dependent on salt concentration and composition, with interfacial affinity potentially varying by orders of magnitude for the same compound under different conditions. The work described here presents a tool for predicting the salt-dependent adsorption of PFAS compounds based entirely on chemical structure, something of great value for predicting the real-world environmental behavior of many of the large numbers of PFAS compounds for which experimental data are not available. The approach combines two different previously-developed models, one a mass-action model designed to predict the effects of salts on interfacial adsorption of ionic PFAS (the UNSW-OU salt model), and the second a group-contribution model designed to predict interfacial adsorption of PFAS in the absence of salt based on chemical structure. The challenge of combining the two models comes from the fact that both are based on different isotherms. The salt model can produce sigmoidal isotherms under salt-limited conditions (an isotherm shape that is supported by experimental evidence), while the group-contribution model can generate Langmuir parameters from calculations based on chemical structure. Equations were derived to determine salt model isotherm parameters from Langmuir parameters (either from the group-contribution model or experimental sources) by matching surface tension curves in the vicinity of the concentration of highest second derivative. Refined group-contribution model parameters were determined based on data from an additional 40 surface tension curves to allow improved structure-based predictions for important classes of PFAS that were not sufficiently well-represented in the original model. The resulting equations provide a tool allowing quantitative predictions of PFAS behavior under realistic environmental conditions for compounds for which little or no experimental data are available.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157663DOI Listing
July 2022

A multimodal model fusing multiphase contrast-enhanced CT and clinical characteristics for predicting lymph node metastases of pancreatic cancer.

Phys Med Biol 2022 Jul 29. Epub 2022 Jul 29.

School of Biomedical Engineering, Guangdong Provincial Key Laboratory of Medical Image Processing, Guangdong Province Engineering Laboratory for Medical Imaging and Diagnostic Technology, Southern Medical University, No. 1838 Guangzhou Avenue North, Guangzhou, Guangdong, 510515, CHINA.

Objective: To develop a multimodal model that combines multiphase contrast-enhanced computed tomography (CECT) imaging and clinical characteristics, including experts' experience, to preoperatively predict lymph node metastasis in pancreatic cancer patients.

Methods: We proposed a new classifier fusion strategy based on a new evidential reasoning rule (CFS-nER) by combining nomogram weights into a previous evidential reasoning rule-based classifier fusion strategy. Three kernelled support tensor machine-based classifiers with plain, arterial, and venous phases of CECT as the inputs, respectively, were constructed. They were then fused based on the CFS-nER to construct a fusion model of multiphase CECT. The clinical characteristics were analyzed by univariate and multivariable logistic regression to screen risk factors, which were used to construct correspondent risk factor-based classifiers. Finally, the fusion model of the three phases of CECT and each risk factor-based classifier were fused further to construct the multimodal model based on our CFS-nER, named MMM-nER. This study consisted of 186 patients diagnosed with pancreatic cancer from four clinical centers in China, 88 (47.31%) of whom had lymph node metastasis.

Results: The fusion model of the three phases of CECT performed better overall than single and two-phase fusion models; this implies that the three considered phases of CECT were supplementary and complemented one another. The MMM-nER further improved the predictive performance, which implies that our MMM-nER can complement the supplementary information between CECT and clinical characteristics. The MMM-nER had better predictive performance than based on previous classifier fusion strategies, which presents the advantage of our CFS-nER.

Conclusion: We proposed a new CFS-nER, based on which the fusion model of the three phases of CECT and MMM-nER were constructed and performed better than all compared methods. MMM-nER achieved an encouraging performance, implying that it can assist clinicians in noninvasively and preoperatively evaluating the lymph node status of pancreatic cancer.
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http://dx.doi.org/10.1088/1361-6560/ac858eDOI Listing
July 2022

Application of a traction metal clip with a fishhook-like device in wound sutures after endoscopic resection.

Clin Endosc 2022 Jul 28;55(4):525-531. Epub 2022 Jul 28.

Department of Gastroenterology, Jiangyin Hospital Affiliated to Nantong University, Jiangyin, China.

Background/aims: Endoscopic wound suturing is an important factor that affects the ability to remove large and full-thickness lesions during endoscopic resection. We aimed to evaluate the effect of a traction metal clip with a fishhook-like device on wound sutures after endoscopic resection.

Methods: From July 2020 to April 2021, patients who met the enrollment criteria were treated with a fishhook-like device during the operation to suture the postoperative wound (group A). Patients with similar conditions and similar size wounds who were treated with a "purse-string suture" to suture the wounds were retrospectively analyzed as the control group (group B). Difference in the suture rate, adverse events, time required for suturing, and number of metal clips were compared between the two groups.

Results: The time required for suturing was 7.72±0.51 minutes in group A and 11.50±0.91 minutes in group B. This difference was statistically significant (F=13.071, p=0.001). The number of metal clamps used in group A averaged 8.1 pieces/case, and the number of metal clamps used in group B averaged 7.3 pieces/case. This difference was not statistically significant (F=0.971, p>0.05).

Conclusion: The traction metal clip with the fishhook-like device is ingeniously designed and easy to operate. It has a good suture effect on the wound after endoscopic submucosal dissection and effectively prevents postoperative adverse events.
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http://dx.doi.org/10.5946/ce.2021.241DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329651PMC
July 2022

Investigation of the Stability and Hydrogen Evolution Activity of Dual-Atom Catalysts on Nitrogen-Doped Graphene.

Nanomaterials (Basel) 2022 Jul 25;12(15). Epub 2022 Jul 25.

Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.

Single atom catalysts (SACs) have received a lot of attention in recent years for their high catalytic activity, selectivity, and atomic utilization rates. Two-dimensional N-doped graphene has been widely used to stabilize transition metal (TM) SACs in many reactions. However, the anchored SAC could lose its activity because of the too strong metal-N interaction. Alternatively, we studied the stability and activity of dual-atom catalysts (DACs) for 24 TMs on N-doped graphene, which kept the dispersion state but had different electronic structures from SACs. Our results show that seven DACs can be formed directly compared to the SACs. The others can form stably when the number of TMs is slightly larger than the number of vacancies. We further show that some of the DACs present better catalytic activities in hydrogen evolution reaction (HER) than the corresponding SACs, which can be attributed to the optimal charge transfer that is tuned by the additional atom. After the screening, the DAC of Re is identified as the most promising catalyst for HER. This study provides useful information for designing atomically-dispersed catalysts on N-doped graphene beyond SACs.
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http://dx.doi.org/10.3390/nano12152557DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332772PMC
July 2022

MBD1/HDAC3-miR-5701-FGFR2 axis promotes the development of gastric cancer.

Aging (Albany NY) 2022 Jul 22;14(14):5878-5894. Epub 2022 Jul 22.

Institute of Genetics and Developmental Biology, Translational Medicine Institute, Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, P.R. China.

Gastric cancer (GC) remains one of the leading causes of cancer-related deaths worldwide due to the lack of specific biomarkers for the early diagnosis and universal accepted therapy for advanced GC. Lower levels of miR-5701 were found in the GC tissue from the online sequencing data and confirmed in the GC tissues and GC cell lines. Overexpression of miR-5701 inhibited the proliferation and migration of GC cells and promoted the apoptosis of these cells. Bioinformatics analyses and luciferase assay showed that miR-5701 targeted FGFR2, which acted as an oncogene in GC. Nude mice with GC cells overexpressing miR-5701 exhibited smaller tumor sizes and less lung metastases. The miR-5701 expression was directly, transcriptionally inhibited by MBD1 together with HDAC3 by binding together to form a complex. Knocked down MBD1 or HDAC3 increased the miR-5701 expression. These results indicated the potential use of exogenously administered miR-5701 or agents that elevated endogenous miR-5701 to inhibit GC, improving the prognosis of patients with GC.
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http://dx.doi.org/10.18632/aging.204190DOI Listing
July 2022

An efficient deep equilibrium model for medical image segmentation.

Comput Biol Med 2022 Jul 5;148:105831. Epub 2022 Jul 5.

The School of Biomedical Engineering, Health Science Center, Shen zhen University, Shenzhen, 518060, China; Shenzhen Key Laboratory of Precision Medicine for Hematological Malignancies, Shenzhen 518060, China; Marshall Laboratory of Biomedical Engineering, Shenzhen 518060, China; Pengcheng Laboratory, Shenzhen 518066, China. Electronic address:

In this paper, we propose an effective method that takes the advantages of classical methods and deep learning technology for medical image segmentation through modeling the neural network as a fixed point iteration seeking for system equilibrium by adding a feedback loop. In particular, the nuclear segmentation of medical image is used as an example to demonstrate the proposed method where it can successfully complete the challenge of segmenting nuclei from cells in different histopathological images. Specifically, the nuclei segmentation is formulated as a dynamic process to search for the system equilibrium. Starting from an initial segmentation generated either by a classic algorithm or pre-trained deep learning model, a sequence of segmentation output is created and combined with the original image to dynamically drive the segmentation towards the expected value. This dynamical extension to neural networks requires little extra change on the backbone deep neural network while it significantly increased model accuracy, generalizability, and stability as demonstrated by intensive experimental results from pathological images of different tissue types across different open datasets.
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http://dx.doi.org/10.1016/j.compbiomed.2022.105831DOI Listing
July 2022

Full-Scale of a Compost Process Using Swine Manure, Human Feces, and Rice Straw as Feedstock.

Front Bioeng Biotechnol 2022 1;10:928032. Epub 2022 Jul 1.

Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin, China.

Regarding the composting of rural waste, numerous studies either addressed the composting of a single waste component or were conducted at a laboratory/pilot scale. However, far less is known about the mixed composting effect of multi-component rural waste on a large scale. Here, we examined nutrient transformation, maturity degree of decomposition, and succession of microbial communities in large-scale (1,000 kg mixed waste) compost of multi-component wastes previously optimized by response models. The results showed that multi-component compost can achieve the requirement of maturity and exhibit a higher nutritional value in actual compost. It is worth noting that the mixed compost effectively removed pathogenic fungi, in which almost no pathogenic fungi were detected, and only two pathogenic bacteria regrown in the cooling and maturation stages. Structural equation models revealed that the maturity (germination index and the ratio of ammonium to nitrate) of the product was directly influenced by compost properties (electrical conductivity, pH, total organic carbon, moisture, temperature, and total nitrogen) compared with enzymes (cellulase, urease, and polyphenol oxidase) and microbial communities. Moreover, higher contents of total phosphorus, nitrate-nitrogen, and total potassium were conducive to improving compost maturity, whereas relatively lower values of moisture and pH were more advantageous. In addition, compost properties manifested a remarkable indirect effect on maturity by affecting the fungal community ( and ). Collectively, this evidence implies that mixed compost of multi-component rural waste is feasible, and its efficacy can be applied in practical applications. This study provides a solution for the comprehensive treatment and utilization of rural waste.
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http://dx.doi.org/10.3389/fbioe.2022.928032DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9286457PMC
July 2022

A prospective randomized clinical trial of active-fluidics versus gravity-fluidics system in phacoemulsification for age-related cataract (AGSPC).

Ann Med 2022 12;54(1):1977-1987

Medical School of Chinese People's Liberation Army, Beijing, China.

Background: To figure out the efficacy, effects, safety and patient's subjective perceptions of phacoemulsification with the active-fluidics system (AFS).

Patients And Methods: This was a prospective, randomized, double-masked, controlled clinical study. Age-related cataract patients were recruited and randomly assigned to the AFS group and gravity-fluidics system (GFS) group in a ratio of 1:1 to have phacoemulsification. Participants were followed up at one day, one week, one month and three months postoperatively (Chinese Clinical Trial Registry, ChiCTR2100044409).

Results: The overall included participants were 107 finally. The total aspiration time of the AFS group was significantly less than that of the GFS group ( = .020), while no significant difference existed in cumulative dissipated energy and estimated fluid usage between the two groups. The best corrected visual acuity was significantly better in the AFS group at one day and one week postoperatively ( = .002,  = .038 respectively). The recovery of central corneal thickening and macular superficial vasculature increase was earlier in the AFS group. The central retinal thickness was significantly higher in the GFS group at one month and three months postoperatively ( = .029,  = .016 respectively). The incidence of corneal adverse events was higher in GFS group ( = .035). No serious adverse events occurred in either group. Pain scores and the scores of Cat-PROM5 questionnaire of the AFS group were significantly lower than that of the GFS group ( = .011,  = .002 respectively).

Conclusion: AFS improves the efficiency, effects, safety and patients' subjective perceptions of phacoemulsification compared with GFS. It is worthwhile to promote its application in cataract surgery.KEY MESSAGESThe active-fluidics system automatically detects and maintains stable intraocular pressure at the set value.The active-fluidics system improves the efficiency, effects, safety and patients' subjective perceptions in phacoemulsification.
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http://dx.doi.org/10.1080/07853890.2022.2098375DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9310653PMC
December 2022

A bioartificial transgenic porcine whole liver expressing human proteins alleviates acute liver failure in pigs.

Hepatobiliary Pancreat Dis Int 2022 Jun 30. Epub 2022 Jun 30.

General Surgery Center, Department of Hepatobiliary Surgery II, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Guangzhou Clinical Research and Transformation Center for Artificial Liver, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China. Electronic address:

Background: Preventing heterologous protein influx in patients is important when using xenogeneic bioartificial livers (BALs) to treat liver failure. The development of transgenic porcine livers synthesizing human proteins is a promising approach in this regard. Here, we evaluated the safety and efficacy of a transgenic porcine liver synthesizing human albumin (hALB) and coagulation factor VII (hFVII) within a bioartificial system.

Methods: Tibetan miniature pigs were randomly subjected to different interventions after surgery-induced partially ischemic liver failure. Group A (n = 4) was subjected to basic treatment; group B (n = 4) was to standard medical treatment and wild-type porcine BAL perfusion, and group C (n = 2) was to standard medical treatment and transgenic BAL perfusion. Biochemical parameters, coagulation status, survival time, and pathological changes were determined. Expressions of hALB and hFVII were detected using immunohistochemistry and enzyme-linked immunosorbent assays.

Results: The survival time in group A was 9.75 ± 1.26 days; this was shorter than that in both perfused groups, in which all animals reached an endpoint of 12 days (P = 0.006). Ammonia, bilirubin, and lactate levels were significantly decreased, whereas albumin and fibrinogen levels were increased after perfusion (all P < 0.05). hALB and hFVII were detected in transgenic BAL-perfused pig serum and ex vivo in the liver tissues.

Conclusions: The humanized transgenic pig livers could synthesize and secrete hALB and hFVII ex vivo in a whole organ-based bioartificial system, while maintaining their metabolism, detoxification, transformation, and excretion functions, which were comparable to those observed in wild-type porcine livers. Therefore, the use of transgenic bioartificial whole livers is expected to become a new approach in treating acute liver failure.
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http://dx.doi.org/10.1016/j.hbpd.2022.06.012DOI Listing
June 2022

Mathematical Modelling and Hierarchical Encourage Particle Swarm Optimization Genetic Algorithm for Jet Pipe Servo Valve.

Comput Intell Neurosci 2022 1;2022:9155248. Epub 2022 Jul 1.

School of Electronic Engineering, Xi'an Shiyou University, No. 18, East Section of Electronic 2nd Road, Xi'an, Shannxi, China.

The jet pipe servo (JPS) valve is one key component, whose dynamic performance directly influences the aircraft's maneuverability. In this paper, a more accurate mathematical model and a novel multiobjective hierarchical encourage particle swarm optimization genetic algorithm (HEPGA) are proposed to improve the dynamic performance of the jet pipe servo valve. By optimizing the main structure parameters of the jet pipe servo valve, the adjustment and overshoot in the dynamic performance are reduced by 24.28% and 51.39%, respectively, compared with the prototype before optimization. To obtain a more accurate mathematical model, the computational fluid dynamics (CFD) is introduced to modify the analytical model considering the turbulent submerged free jet. Different from conventional numerical simulation, the dynamic mesh technique is used to analyze the flow field distribution by considering the force interaction of various parts of the jet pipe servo valve under actual working condition. Then, the HEPGA with better convergence is utilized because of the conflict of adjustment and overshoot. This proposed hybrid algorithm introduces the concept of staff welfare system to divide the population into elite individuals and excellent individuals of particle swarm optimization and general individuals of genetic algorithm. Meanwhile, the convergency performance of the HEPGA is evaluated through the Rosenbrock function by comparing with other particle swarm genetic hybrid methods. Subsequently, the experimental platform is constructed and the dynamic performance tests are conducted on the prototype after optimization. The experimental results verify the accuracy of the established mathematical model and the significant improvement of dynamic performance of the jet pipe servo valve.
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http://dx.doi.org/10.1155/2022/9155248DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9270145PMC
July 2022

QNE1 is a key flowering regulator determining the length of the vegetative period in soybean cultivars.

Sci China Life Sci 2022 Jul 1. Epub 2022 Jul 1.

State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, 100101, China.

The soybean E1 gene is a major regulator that plays an important role in flowering time and maturity. However, it remains unclear how cultivars carrying the dominant E1 allele adapt to the higher latitudinal areas of northern China. We mapped the novel quantitative trait locus QNE1 (QTL near E1) for flowering time to the region proximal to E1 on chromosome 6 in two mapping populations. Positional cloning revealed Glyma.06G204300, encoding a TCP-type transcription factor, as a strong candidate gene for QNE1. Association analysis further confirmed that functional single nucleotide polymorphisms (SNPs) at nucleotides 686 and 1,063 in the coding region of Glyma.06G204300 were significantly associated with flowering time. The protein encoded by the candidate gene is localized primarily to the nucleus. Furthermore, soybean and Brassica napus plants overexpressing Glyma.06G204300 exhibited early flowering. We conclude that despite their similar effects on flowering time, QNE1 and E4 may control flowering time through different regulatory mechanisms, based on expression studies and weighted gene co-expression network analysis of flowering time-related genes. Deciphering the molecular basis of QNE1 control of flowering time enriches our knowledge of flowering gene networks in soybean and will facilitate breeding soybean cultivars with broader latitudinal adaptation.
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http://dx.doi.org/10.1007/s11427-022-2117-xDOI Listing
July 2022

Reliability assessment of the upper urinary tract dilation grading system based on magnetic resonance urography in patients with neurogenic bladder.

BMC Urol 2022 Jul 4;22(1):97. Epub 2022 Jul 4.

Cheeloo College of Medicine, Shandong University; Department of Urology, China Rehabilitation Research Center, Beijing, 100068, China.

Background: To assess the inter-observer and intra-observer reliability of the magnetic resonance urography (MRU)-upper urinary tract dilation (UUTD) grading system.

Methods: A total of 40 patients with a diagnosis of NB were enrolled in this study. The images were assembled in an electronic presentation randomly. The presentations were reviewed and graded by 4 junior and 4 senior urologists. One week later, the images were randomized again and reassessed. The inter-observer reliability was estimated by Kendall's coefficient of concordance and intra-class correlation coefficient (ICC), and the intra-observer reliability was estimated by weighted Cohen's kappa.

Results: The inter-observer reliability strength was excellent for all urologists, with the ICC value of 0.939 (0.908-0.963) and Kendall's W value of 0.967. The highest agreement was shown in Grade 4 at 92.50%, and the lowest in Grade 2 at 82.14%. All disagreements were within one grade of difference. Moreover, the Intra-observer reliability was excellent, with the weighted kappa value ranging from 0.904 to 0.954.

Conclusions: The inter-observer and intra-observer reliability of this novel MRU-UUTD grading system is confirmed, providing adequate evidence for broader clinical application.
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http://dx.doi.org/10.1186/s12894-022-01039-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9254662PMC
July 2022

Low concentration triphenyl phosphate fuels proliferation and migration of hepatocellular carcinoma cells.

Environ Toxicol 2022 Jul 1. Epub 2022 Jul 1.

General Surgery Center, Department of Hepatobiliary Surgery II, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Guangzhou Clinical Research and Transformation Center for Artificial Liver Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China.

Organophosphate flame retardants (OPFRs) have been widely used due to their unique properties. The OPFRs are mainly metabolized in the liver. However, whether the plasma level of OPFRs was involved in the progression of liver cancer remains unclear. Triphenyl phosphate (TPP) is one of the OPFRs that are mostly detected in environment. In this study, we performed CCK8, ATP, and EdU analyses to evaluate the effect of TPP at the concentrations at 0.025-12.8 μM on the proliferation, invasion, and migration of Hep3B, a hepatocellular carcinoma (HCC) cell line. Tumor-bearing mouse model was used for in vivo validation. The results showed that low concentrations of TPP at (0.025-0.1 μM), which are obtained in the plasma of patients with cancers, remarkably promoted cell invasion and migration of Hep3B cells. Animal experiments confirmed that TPP treatment significantly enhanced tumor growth in the xenograft HCC model. To explore the possible molecular mechanisms that might mediate the actions of TPP on Hep3B cells, we profiled gene expression in groups treated with or without TPP at the concentrations of 0.05 and 0.1 μM using transcriptional sequencing. Gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment, and Protein-protein interaction (PPI) analyses demonstrated that pathways affected by differentially expressed genes (DEGs) were mainly in nuclear-transcribed mRNA catabolic processes, cytosolic ribosome, and ATPase activity. A 0.05 and 0.1 μM TPP led to up-regulation of a series of genes including EREG, DNPH1, SAMD9, DUSP5, PFN1, CKB, MICAL2, SCUBE3, and CXCL8, but suppressed the expression of MCC. These genes have been shown to be associated with proliferation and movement of cells. Taken together, our findings suggest that low concentration of TPP could fuel the proliferation, invasion, and migration of HCC cells. Thus, TPP is a risk factor in the progression of HCC in human beings.
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http://dx.doi.org/10.1002/tox.23609DOI Listing
July 2022

Simultaneous resection of coexisting pulmonary and mediastinal lesions by video-assisted thoracic surgery: a case-series study.

BMC Surg 2022 Jun 20;22(1):237. Epub 2022 Jun 20.

Department of Thoracic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei, People's Republic of China.

Background: With the growing number of patients with coexisting pulmonary and mediastinal lesions detected, reports about simultaneous video-assisted thoracic surgery (VATS) for these concurrent diseases are still rare. To further explore the safety and effectiveness of simultaneous resection of pulmonary and mediastinal lesions by uniportal or biportal VATS, we retrospectively analyzed the clinical data of the largest series of cases to date.

Methods: From July 2018 to July 2021, all patients whose pulmonary lesions and mediastinal tumors were resected simultaneously in our institution were retrospectively reviewed. Their demographic and clinical data were collected and analyzed.

Results: A total of 54 patients were enrolled, of whom 44 underwent unilateral uniportal VATS, 3 underwent bilateral uniportal VATS and 7 underwent unilateral biportal VATS. Seven cases were converted to thoracotomy during surgery. For the remaining 47 patients with various demographic and clinical characteristics, most of the operations were completed within 3 h (n = 33, 70.2%) with blood loss of no more than 100 mL (n = 43, 91.5%). The duration of chest tube drainage was 5.66 ± 3.34 days, and the average daily volume was 196.90 ± 122.31 mL. Four cases of postoperative complications occurred during hospitalization. The length of postoperative hospital stay was 8.60 ± 3.63 days. No severe complications or deaths were observed during follow-up.

Conclusions: Uniportal and biportal VATS are safe and effective for simultaneous resection of selected coexisting pulmonary and mediastinal lesions, but the indications and operational details need more evaluation.
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http://dx.doi.org/10.1186/s12893-022-01684-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208703PMC
June 2022

bFGF-Loaded Mesoporous Silica Nanoparticles Promote Bone Regeneration Through the Wnt/β-Catenin Signalling Pathway.

Int J Nanomedicine 2022 7;17:2593-2608. Epub 2022 Jun 7.

School of Medicine, Southern University of Science and Technology, Shenzhen, 518055, People's Republic of China.

Background: Bone defects remain an unsolved clinical problem due to the lack of effective osteogenic induction protocols. Nanomaterials play an important role in bone defect repair by stimulating osteogenesis. However, constructing an effective bioactive nanomaterial remains a substantial challenge.

Methods: In this study, mesoporous silica nanoparticles (MSNs) were prepared and used as nanocarriers for basic fibroblast growth factor (bFGF). The characteristics and biological properties of the synthetic [email protected] were tested. The osteogenic effects of the particles on the behavior of MC3T3-E1 cells were investigated in vitro. In addition, the differentially expressed genes during induction of osteogenesis were analyzed by transcriptomic sequencing. Radiological and histological observations were carried out to determine bone regeneration capability in a distal femur defect model.

Results: Achieving bFGF sustained release, [email protected] had uniform spherical morphology and good biocompatibility. In vitro osteogenesis induction experiments showed that [email protected] exhibited excellent osteogenesis performance, with upregulation of osteogenesis-related genes (RUNX2, OCN, Osterix, ALP). Transcriptomic sequencing revealed that the Wnt/β-catenin signalling pathway could be activated in regulation of biological processes. In vivo, bone defect repair experiments showed enhanced bone regeneration, as indicated by radiological and histological analysis, after the application of [email protected]

Conclusion: [email protected] can promote bone regeneration by activating the Wnt/β-catenin signalling pathway. These particles are expected to become a potential therapeutic bioactive material for clinical application in repairing bone defects in the future.
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http://dx.doi.org/10.2147/IJN.S366926DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9188412PMC
June 2022

Hepatoprotective and Human Gut Microbiota Modulation of Polysaccharide-Peptides in .

Front Cell Infect Microbiol 2022 20;12:892049. Epub 2022 May 20.

Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.

, a golden oyster mushroom, is popular in Asia and has pharmacological functions. However, the effects of polysaccharide-peptides extracted from and underlying mechanism on digestive systme have not yet been clarified. Here, we determined the composition of two polysaccharide-peptides (PSI and PSII) from and investigated the protective effects of on hepatoprotective and gut microbiota. The results showed that PSI and PSII were made up of similar monosaccharide moieties, except for the varying ratios. Furthermore, PSI and PSII showed that they have the hepatoprotective effects and significantly increased the viabilities and cellular total superoxide dismutase activities increased significantly in HepG2 cells. Intracellular triglyceride content and extracellular alanine aminotransferase and aspartate transaminase contents markedly decreased following treatment with 40 and 50 μg/mL PSI and PSII, respectively. Moreover, PSI and PSII activated the adiponectin pathway and reduced lipid accumulation in liver cells. PSI and PSII elevated short-chain fatty acid concentrations, especially butyric and acetic acids. 16S rRNA gene sequencing analysis showed that PSI promoted the relative abundances of , , , as well as generas in the gut. PSII markedly suppressed the relative abundances of and generas. We speculate that the PSI and PSII play a role through liver-gut axis system. Polysaccharide-peptides metabolize by gut microbiota to produce short-chain fatty acids (SCFAs) and in turn influence liver functions.
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http://dx.doi.org/10.3389/fcimb.2022.892049DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9165600PMC
May 2022

Enhanced Sampling Simulation Reveals How Solvent Influences Chirogenesis of the Intra-Molecular Diels-Alder Reaction.

J Chem Theory Comput 2022 Jul 6;18(7):4318-4326. Epub 2022 Jun 6.

Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.

The timescale involved in chemical reactions is quite often beyond that of normal molecular dynamics simulations. Here, we combine metadynamics with selective integrated tempering sampling to simulate an intra-molecular Diels-Alder reaction in explicit solvents. Based on a one-dimensional collective variable obtained from harmonic linear discriminant analysis, four chiral isomers of products were observed in the simulation. Analyses of reactive trajectories showed that this reaction follows a concerted mechanism in all four solvents. In addition, the hydrogen bond between the reactant and water solvent plays an important role in the water-accelerated reaction mechanism. The dynamics of chirality formation varies significantly with solvents. The chirality of products forms significantly before the transition state, especially in ionic liquid.
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http://dx.doi.org/10.1021/acs.jctc.2c00233DOI Listing
July 2022

Risk factors for impaired pulmonary diffusion function in convalescent COVID-19 patients: A systematic review and meta-analysis.

EClinicalMedicine 2022 Jul 27;49:101473. Epub 2022 May 27.

State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510120, Guangdong, China.

Background: The long-term prognosis of COVID-19 survivors remains poorly understood. It is evidenced that the lung is the main damaged organ in COVID-19 survivors, most notably in impairment of pulmonary diffusion function. Hence, we conducted a meta-analysis of the potential risk factors for impaired diffusing capacity for carbon monoxide (DLCO) in convalescent COVID-19 patients.

Methods: We performed a systematic search of PubMed, Web of Science, Embase, and Ovid databases for relevant studies from inception until January 7, 2022, limited to papers involving human subjects. Studies were reviewed for methodological quality. Fix-effects and random-effects models were used to pool results. Heterogeneity was assessed using I. The publication bias was assessed using the Egger's test. PROSPERO registration: CRD42021265377.

Findings: A total of eighteen qualified articles were identified and included in the systematic review, and twelve studies were included in the meta-analysis. Our results showed that female (OR: 4.011; 95% CI: 2.928-5.495), altered chest computerized tomography (CT) (OR: 3.002; 95% CI: 1.319-6.835), age (OR: 1.018; 95% CI: 1.007-1.030), higher D-dimer levels (OR: 1.012; 95% CI: 1.001-1.023) and urea nitrogen (OR: 1.004;95% CI: 1.002-1.007) were identified as risk factors for impaired DLCO.

Interpretation: Pulmonary diffusion capacity was the most common impaired lung function in recovered patients with COVID-19. Several risk factors, such as female, altered chest CT, older age, higher D-dimer levels and urea nitrogen are associated with impairment of DLCO. Raising awareness and implementing interventions for possible modifiable risk factors may be valuable for pulmonary rehabilitation.

Funding: This work was financially supported by Emergency Key Program of Guangzhou Laboratory (EKPG21-29, EKPG21-31), Incubation Program of National Science Foundation for Distinguished Young Scholars by Guangzhou Medical University (GMU2020-207).
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http://dx.doi.org/10.1016/j.eclinm.2022.101473DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9137275PMC
July 2022

Quality inspection and result analysis of the spirometer calibration cylinder.

BMC Pulm Med 2022 Jun 4;22(1):218. Epub 2022 Jun 4.

National Center for Respiratory Medicine, State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, First Affiliated Hospital of Guangzhou Medical University, Yanjiang Road 151, Guangzhou, 510120, People's Republic of China.

Background: To understand the accuracy of volume calibration syringes used in China and compare the difference between new and old volume calibration syringes, technical testing was performed on volume calibration syringes in clinical lung function instruments.

Materials And Methods: A standard validator device (Model 1180, Hans Rudolph, USA) was used to perform leak testing and volume accuracy testing for calibration syringes. Sixteen volume calibration syringes from 8 brands (CareFusion in Germany, Vyaire in Germany, Yaeger in Germany, Vitalograph in the United Kingdom, MGC Diagnostics in the United States, U-Breath in Zhejiang, China, Wendi in Ningbo, Zhejiang, and Boya in Ningbo, China) were tested.

Results: A total of 75% (12/16) of the volume calibration syringes passed the pressure decay leak test, 69% (11/16) of the volume calibration syringes passed the volume accuracy and repeatability test, and 56% (9/16) passed both tests; there was no significant difference in the total passing of the new and old volume calibration syringe quality tests (P > 0.05).

Conclusions: A standard validator device should be used for both leakage tests and volume accuracy and repeatability tests to ensure the reliability of volume calibration syringes. It is suggested that the quality verification of volume calibration syringes should be regularly conducted to ensure the accuracy of the pulmonary function tests.
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http://dx.doi.org/10.1186/s12890-022-02010-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9166523PMC
June 2022

Microscopic Insight into pH-Dependent Conformational Dynamics and Noncanonical Base Pairing in Telomeric i-Motif DNA.

J Phys Chem Lett 2022 Jun 3:5109-5115. Epub 2022 Jun 3.

Institute of Systems and Physical Biology, Shenzhen Bay Laboratory, 518107 Shenzhen, China.

Gene regulatory functions of noncanonical i-motif DNA are associated with dynamic i-motif formation in the cellular environment and pH variation. With atomistic simulations, we show the dramatic influence of solvent pH on the conformational dynamics of biologically relevant telomeric i-motif DNA coupled with protonation of cytosine bases in different conformations. We rationalized the pH-dependent dynamics and conformational variability of the i-motif in terms of base pairing and specific loop motions. The human telomeric i-motif is found to acquire various metastable folded conformations at pH values near the p of cytosine with the formation of a noncanonical C:C W:W trans base pair along with the hemiprotonated C:C pairs in the i-motif core. pH-dependent dynamics and the local solvent structure of i-motif DNA imply that the presence of a cosolvent or molecular crowding can promote i-motif formation by changing the conformational fluctuations and hydration state of the structure.
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http://dx.doi.org/10.1021/acs.jpclett.2c00640DOI Listing
June 2022

LECT2: A pleiotropic and promising hepatokine, from bench to bedside.

J Cell Mol Med 2022 07 3;26(13):3598-3607. Epub 2022 Jun 3.

General Surgery Center, Department of Hepatobiliary Surgery II, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Guangzhou Clinical Research and Transformation Center for Artificial Liver, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China.

LECT2 (leucocyte cell-derived chemotaxin 2) is a 16-kDa protein mainly produced by hepatocytes. It was first isolated in PHA-activated human T-cell leukaemia SKW-3 cells and originally identified as a novel neutrophil chemotactic factor. However, many lines of studies suggested that LECT2 was a pleiotropic protein, it not only functioned as a cytokine to exhibit chemotactic property, but also played multifunctional roles in some physiological conditions and pathological abnormalities, involving liver regeneration, neuronal development, HSC(haematopoietic stem cells) homeostasis, liver injury, liver fibrosis, hepatocellular carcinoma, metabolic disorders, inflammatory arthritides, systemic sepsis and systemic amyloidosis. Among the above studies, it was discovered that LECT2 could be a promising molecular biomarker and therapeutic target. This review summarizes LECT2-related receptors and pathways, basic and clinical researches, primarily in mice and human, for a better comprehension and management of these diseases in the future.
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http://dx.doi.org/10.1111/jcmm.17407DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9258709PMC
July 2022

Effects of soil properties on heavy metal bioavailability and accumulation in crop grains under different farmland use patterns.

Sci Rep 2022 06 2;12(1):9211. Epub 2022 Jun 2.

School of Resources and Environmental Engineering, Anhui University, Hefei, 230061, China.

Mining activities have increased the accumulation of heavy metals in farmland soil and in food crops. To identify the key soil properties influencing heavy metal bioavailability and accumulation in food crops, 81 crop samples and 81 corresponding agricultural soil samples were collected from rape, wheat, and paddy fields. Heavy metal (copper (Cu), zinc (Zn), lead (Pb), cadmium (Cd), iron (Fe), and manganese (Mn)) concentrations in soils and rape, wheat, rice grains were determined using inductively coupled plasma atomic emission spectroscopy, and soil physicochemical properties (pH, organic matter, total nitrogen, total phosphorus, available phosphorus, and available potassium (AK)) were analyzed. Soil extractable metals were extracted using various single extractants (DTPA, EDTA, NHOAc, NHNO, and HCl). The average concentrations of Cu, Zn, Pb, Cd, and Mn in the soil samples all exceeded the local geochemical background value (background values of Cu, Zn, Pb, Cd, and Mn are 43.0, 81.0, 28.5, 0.196, and 616 mg/kg, respectively), and Cd over-standard rate was the highest, at 98%. Furthermore, soil total Cd concentrations (0.1-24.8 mg/kg) of more than 86% of the samples exceeded the soil pollution risk screening value (GB 15618-2018). The sources of Cu, Zn, Pb, Cd, and Mn in soils were mainly associated with mining activities. The key factors influencing heavy metal bioavailability were associated with the types of extractants (complexing agents or neutral salt extractants) and the metals. Cd and Pb concentrations in most wheat and rice grain samples exceeded the maximum allowable Cd and Pb levels in food, respectively, and Cd concentrations in approximately 10% of the rice grain samples exceeded 1.0 mg/kg. Furthermore, rice and wheat grains exhibited higher Cd accumulation capacity than rape grains, and despite the high soil Cd concentrations in the rape fields, the rape grains were safe for consumption. High soil pH and AK restricted Cd and Cu accumulation in wheat grains, respectively. Soil properties seemed to influence heavy metal accumulation in rice grains the most.
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http://dx.doi.org/10.1038/s41598-022-13140-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9163331PMC
June 2022

Asymmetrically Doping a Platinum Atom into a Au Nanocluster for Changing the Electron Configuration and Reactivity in Electrocatalysis.

Angew Chem Int Ed Engl 2022 Aug 15;61(31):e202207685. Epub 2022 Jun 15.

Key Lab of Mesoscopic Chemistry of MOE and Jiangsu Key Lab of Vehicle Emissions Control, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, China.

It is an obstacle to precisely manipulate a doped heteroatom into a desired position in a metal nanocluster. Herein, we overcome this difficulty to obtain Pt Au (SCH Ph Bu) and Pt Au (SCH Ph Bu) nanoclusters via controllably doping Pt atoms into the kernels of Au (SCH Ph Bu) . We reveal that asymmetrical doping of one Pt atom into either of the cores of Au (SCH Ph Bu) elevates the relative energy of the HOMO (highest occupied molecular orbital) accompanied by one valence electron loss of Pt Au (SCH Ph Bu) , compared to Au (SCH Ph Bu) with 14 electrons, while symmetrical doping of two Pt atoms into the cores of Au (SCH Ph Bu) narrows the HOMO-LUMO gap (LUMO: lowest unoccupied molecular orbital) of Pt Au (SCH Ph Bu) with two valence electrons less. Consequently, Pt Au (SCH Ph Bu) shows an electron-spin-induced high activity for CO electroreduction, whereas Pt Au (SCH Ph Bu) is least efficient and Au (SCH Ph Bu) has a decent performance.
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http://dx.doi.org/10.1002/anie.202207685DOI Listing
August 2022

Synthesis of PNIPAAm-P4VP Microgel as Draw Agent in Forward Osmosis by RAFT Polymerization and Reverse Suspension Polymerization to Improve Water Flux.

Molecules 2022 May 17;27(10). Epub 2022 May 17.

School of Materials Science and Engineering, Hainan University, Haikou 570228, China.

Microgels have unique and versatile properties allowing their use in forward osmosis areas as a draw agent. In this contribution, poly(4-vinylpyridine) (P4VP) was synthesized via RAFT polymerization and then grafted to a poly(-Isopropylacrylamide) (PNIPAAm) crosslinking network by reverse suspension polymerization. P4VP was successfully obtained by the quasiliving polymerization with the result of nuclear magnetic resonance and gel permeation chromatography characterization. The particle size and particle size distribution of the PNIPAAm-P4VP microgels containing 0, 5, 10, 15 and 20 wt% P4VP were measured by means of a laser particle size analyzer. It was found that all the microgels were of micrometer scale and the particle size was increased with the P4VP load. Inter/intra-molecular-specific interactions, i.e., hydrogen bond interactions were then investigated by Fourier infrared spectroscopy. In addition, the water flux measurements showed that all the PNIPAAm--P4VP microgels can draw water more effectively than a blank PNIPAAm microgel. For the copolymer microgel incorporating 20 wt% P4VP, the water flux was measured to be 7.48 L∙m∙h.
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http://dx.doi.org/10.3390/molecules27103214DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9146756PMC
May 2022

What role does organic fertilizer actually play in the fate of antibiotic resistome and pathogenic bacteria in planting soil?

J Environ Manage 2022 Sep 24;317:115382. Epub 2022 May 24.

Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, 300191, China. Electronic address:

Organic fertilizer increase antibiotic resistance genes (ARGs) and bacterial pathogens have widely documented. However, how organic fertilizer is involved in changing soil ARGs and pathogenic bacteria after long-term (≥5 years) application remains unclear. Herein, the ARGs and pathogenic bacteria were compared in organic fertilized soils (AF) and non-fertilized soils (NF), and the contribution of input sources (organic fertilizer, irrigation water, air and background soil) on soil ARGs also was determined in this study. Results showed that the abundances of some ARGs, such as vanR and aac(6')-I in AF, were significantly higher than these of NF (p < 0.05). And a relatively higher abundance of potential pathogens, especially, Salmonella enterica and Stenotrophomonas maltophilia, in AF was observed. This indicated that organic fertilizer application can maintain a high level of some soil ARGs and pathogenic bacteria for at least 5 years. Traceability analysis unearthed that organic fertilizer application mainly increased its own contribution to soil ARGs from 1.16% to 9.05%, as well reduced the contribution of background soil, suggesting that the increase in soil ARGs may be partly attributable to organic fertilizer inputs. Notably, organic fertilizer application did not significantly alter the contribution ratio of input sources to microorganisms, but there was a clear change in the composition of soil microorganisms, which meant that the effect of the input source on the microorganism may emanate from other factors, rather than direct inputs. Subsequent structural equation demonstrated that organic fertilizer application significantly enhanced the effect of environmental factors on ARGs, and also indirectly increased the influence of communities on ARGs. Collectively, under the long-term fertilization, the role of organic fertilizers on soil ARGs not just stems from its own input, and also dominates the influence of environmental factors on ARGs. This study elucidates main causes for the difference in ARGs in AF vs. NF and enlightens actual role of organic fertilizer in them.
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http://dx.doi.org/10.1016/j.jenvman.2022.115382DOI Listing
September 2022

Hepatic cortical blindness: a case report and comprehensive literature review.

Am J Med Sci 2022 May 16. Epub 2022 May 16.

Department of Gastroenterology, The Affiliated Hospital of Northwest University, Xi'an No.3 Hospital, Xi'an, Shaanxi, China.

Hepatic cortical blindness is an unusual clinical complication of hepatic encephalopathy, and its epidemiology has not been studied in detail. Herein, we present a case report of a 37-year-old male with liver cirrhosis and immunoglobulin A nephropathy who noted visual impairment after eating high-protein food. In addition to the case report, all previously documented cases of hepatic cortical blindness occurring across the globe published from inception until August 2019 were reviewed. The incidence of hepatic cortical blindness is low, and diagnoses are easily missed. The diagnosis of hepatic cortical blindness is dependent on symptoms, and patients may experience several attacks of vision loss. Most patients experience complete blindness, but vision loss recovers within 10 minutes to one month. Early detection and therapy for hepatic encephalopathy may improve patient outcome.
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http://dx.doi.org/10.1016/j.amjms.2022.04.034DOI Listing
May 2022

Ultraefficient reconstruction of effectively hyperuniform disordered biphase materials via non-Gaussian random fields.

Phys Rev E 2022 Apr;105(4-2):045305

School for Engineering of Matter, Transport & Energy, Arizona State University, Tempe, Arizona 85281, USA.

Disordered hyperuniform systems are statistically isotropic and possess no Bragg peaks like liquids and glasses, yet they suppress large-scale density fluctuations in a similar manner as in perfect crystals. The unique hyperuniform long-range order in these systems endow them with nearly optimal transport, electronic, and mechanical properties. The concept of hyperuniformity was originally introduced for many-particle systems and has subsequently been generalized to biphase heterogeneous materials such as porous media, composites, polymers, and biological tissues for unconventional property discovery. Existing methods for rendering realizations of disordered hyperuniform biphase materials reconstruction typically employ stochastic optimization such as the simulated annealing approach, which requires many iterations. Here, we propose an explicit ultraefficient method for reconstructing effectively hyperuniform biphase materials, based on the second-order non-Gaussian random fields where no additional tuning step or iteration is needed. Both the effectively hyperuniform microstructure and the latent material property field can be simultaneously generated in a single reconstruction. Moreover, our method can also incorporate hierarchical uncertainties in the heterogeneous materials, including both uncertainties in the disordered material microstructure and material property variation within each phase. The efficiency and feasibility of the proposed reconstruction method are demonstrated via a wide spectrum of examples spanning from isotropic to anisotropic, effectively hyperuniform to nonhyperuniform, and antihyperuniform systems. Our ultraefficient reconstruction method can be readily incorporated into material design, probabilistic analysis, optimization, and discovery of novel disordered hyperuniform heterogeneous materials.
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http://dx.doi.org/10.1103/PhysRevE.105.045305DOI Listing
April 2022
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