Publications by authors named "Yi Fang"

640 Publications

Microplastic pollution in soils, plants, and animals: A review of distributions, effects and potential mechanisms.

Sci Total Environ 2022 Aug 3:157857. Epub 2022 Aug 3.

Jilin Provincial Key Laboratory of Grassland Farming, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, Jilin 130102, China. Electronic address:

Increasing production of synthetic plastics and poor management of plastic wastes have dramatically increased the amount of plastics in the environment. In 2014, at the first United Nations Environment Assembly, marine plastic waste pollution was listed as one of the 10 most pressing environmental issues. In addition, there is much plastic waste in terrestrial ecosystems due to substantial residues from agricultural mulching and packing. As a recently recognized pollutant, microplastics (MPs) have attracted significant attention from the public and various governments. Concentrations of MPs in the environment vary among locations, from <100 to >1 × 10 particles per cubic meter. Many studies have addressed the impacts and potential mechanisms of MPs on the environment and organisms. Humans and other organisms can ingest or carry MPs in a variety of passive ways and these MPs can have a range of negative effects on metabolism, function, and health. Additionally, given their large surface area, MPs can sorb various pollutants, including heavy metals and persistent organic pollutants, with serious implications for animals and human wellbeing. However, due to their complexity and a lack of accurate determination methods, the systematic impacts of MP pollution on whole foodwebs are not clearly established. Therefore, this review summarizes current research advances in MP pollution, particularly the impact of MPs on soils, plants, and animals, and proposes potential future research prospects to better characterize MPs.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157857DOI Listing
August 2022

Influence of root distribution patterns on soil dynamic characteristics.

Sci Rep 2022 Aug 4;12(1):13448. Epub 2022 Aug 4.

National Institute of Natural Hazards, Ministry of Emergency Management of China, Beijing, 100083, People's Republic of China.

Slopes along the highway and railway routes are subjected to not only static loads but also dynamic loads generated by vehicles and trains. The induced excessive deformation potentially poses a threat to slope stability. In terms of the extensive application of ecological slope protection, plants play a critical role in slope stability, as the roots can enhance the shear strength of the soil. This study aims to investigate the influence of different root distribution patterns on the dynamic characteristics induced by cyclic loading. By conducting a group of dynamic triaxial tests, the results indicate that the root system can significantly enhance the liquefaction resistance of the soil when the soil is subjected to lower dynamic loads, and the cross arrangement has a better-reinforced effect than the mixed arrangement. The reinforced effect was not obvious when the soil was subjected to a dynamic load with a larger stress amplitude. In addition, based on the validation of the seed model, a new pore water pressure development model was proposed according to the test results. Overall, the research provides a new model and some innovative observations to better understand the dynamic behavior of root-reinforced soil.
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http://dx.doi.org/10.1038/s41598-022-17828-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9352746PMC
August 2022

Enzymatic hydrolysis of corn stover lignin by laccase, lignin peroxidase, and manganese peroxidase.

Bioresour Technol 2022 Jul 26;361:127699. Epub 2022 Jul 26.

Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China. Electronic address:

Lignin of high purity and structural integrity was isolated from the enzymatic residue of corn stover. Degradation of the lignin by laccase, lignin peroxidase, and manganese peroxidase was investigated. Structural changes in the lignin after degradation were characterized by scanning electron microscopy, nitrogen adsorption and Fourier transform infrared spectroscopy, and the enzymatic products were systematically analyzed by gas chromatography mass spectrometry. The highest percentage of lignin degradation was obtained with a mixture of three enzymes (25.79%): laccase (Lac), the starting enzyme of the mixed enzyme reaction, worked with lignin peroxidase (LiP), and manganese peroxidase (MnP) to further degrade lignin. This degradation destroyed the macromolecular structure of lignin, broke its key chemical bonds, and opened benzene rings, thus producing more acidic compounds. This study elucidated the concept of degrading lignin from corn stover using the Lac, LiP and MnP enzymes synergistically, thus providing a theoretical basis for the biodegradation of lignin.
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http://dx.doi.org/10.1016/j.biortech.2022.127699DOI Listing
July 2022

Correction: Long-term drinking behavior change patterns and its association with hyperuricemia in chinese adults: evidence from China Health and Nutrition Survey.

BMC Public Health 2022 Jul 26;22(1):1418. Epub 2022 Jul 26.

Department of Nephrology, Zhongshan Hospital, Fudan University, No.180 Fenglin Road, Shanghai, 200032, Shanghai, China.

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http://dx.doi.org/10.1186/s12889-022-13794-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316682PMC
July 2022

Effects of Hyperbaric Exposure on Hematology and Cecal Microbiota in Rats.

Biomed Environ Sci 2022 06;35(6):538-542

College of Food Science and Engineering, Jilin University, Changchun 130062, Jilin, China.

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http://dx.doi.org/10.3967/bes2022.071DOI Listing
June 2022

Characterization of Lactate Metabolism Score in Breast and Thyroid Cancers to Assist Immunotherapy via Large-Scale Transcriptomic Data Analysis.

Front Pharmacol 2022 7;13:928419. Epub 2022 Jul 7.

Department of Breast Surgical Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Breast cancer (BC) and thyroid cancer (TC) have the highest rate of incidence, especially in women. Previous studies have revealed that lactate provides energetic and anabolic support to cancer cells, thus serving as an important oncometabolite with both extracellular and intracellular signaling functions. However, the correlation of lactate metabolism scores with thyroid and breast cancer immune characteristics remains to be systematically analyzed. To investigate the role of lactate at the transcriptome level and its correlation with the clinical outcome of BC and TC, transcriptome data of 1,217 patients with breast cancer (BC) and 568 patients with thyroid cancer (TC) were collected from The Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) datasets with their corresponding clinical and somatic mutation data. The lactate metabolism score was calculated based on a single-sample gene set enrichment analysis (ssGSEA). The results showed that lactate metabolism-related genes and lactate metabolism scores was significantly associated with the survival of patients with BRCA and THCA. Notably, the lactate metabolism scores were strongly correlated with human leukocyte antigen (HLA) expression, tumor-infiltrating lymphocyte (TIL) infiltration, and interferon (IFN) response in BC and TC. Furthermore, the lactate metabolism score was an independent prognostic factor and could serve as a reliable predictor of overall survival, clinical characteristics, and immune cell infiltration, with the potential to be applied in immunotherapy or precise chemotherapy of BC and TC.
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http://dx.doi.org/10.3389/fphar.2022.928419DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301074PMC
July 2022

Amelioration of Non-Alcoholic Steatohepatitis by Polysaccharide, Chlorogenic Acid, and Geniposide Combination Is Associated With Reducing Endotoxin Gut Leakage.

Front Cell Infect Microbiol 2022 5;12:827516. Epub 2022 Jul 5.

Institute of Liver Diseases, Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Gut-derived lipopolysaccharide (LPS) leaking through the dysfunctional intestinal barrier contributes to the onset of non-alcoholic steatohepatitis (NASH) by triggering inflammation in the liver. In the present study, a combination consisting of polysaccharide (A), chlorogenic acid (C), and geniposide (G) (together, ACG), was shown to ameliorate NASH in mice and reduce hepatic LPS signaling and endotoxemia without decreasing the abundance of identified Gram-negative bacteria through restoring the intestinal tight junctions. Our data indicated that inhibition of LPS gut leakage by the ACG combination contributed to its amelioration of NASH.
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http://dx.doi.org/10.3389/fcimb.2022.827516DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294165PMC
July 2022

A Convolutional Neural Network Model for Detecting Sellar Floor Destruction of Pituitary Adenoma on Magnetic Resonance Imaging Scans.

Front Neurosci 2022 4;16:900519. Epub 2022 Jul 4.

Department of Neurosurgery, Dongfang Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, China.

Objective: Convolutional neural network (CNN) is designed for image classification and recognition with a multi-layer neural network. This study aimed to accurately assess sellar floor invasion (SFI) of pituitary adenoma (PA) using CNN.

Methods: A total of 1413 coronal and sagittal magnetic resonance images were collected from 695 patients with PAs. The enrolled images were divided into the invasive group ( = 530) and the non-invasive group ( = 883) according to the surgical observation of SFI. Before model training, 100 images were randomly selected for the external testing set. The remaining 1313 cases were randomly divided into the training and validation sets at a ratio of 80:20 for model training. Finally, the testing set was imported to evaluate the model performance.

Results: A CNN model with a 10-layer structure (6-layer convolution and 4-layer fully connected neural network) was constructed. After 1000 epoch of training, the model achieved high accuracy in identifying SFI (97.0 and 94.6% in the training and testing sets, respectively). The testing set presented excellent performance, with a model prediction accuracy of 96%, a sensitivity of 0.964, a specificity of 0.958, and an area under the receptor operator curve (AUC-ROC) value of 0.98. Four images in the testing set were misdiagnosed. Three images were misread with SFI (one with conchal type sphenoid sinus), and one image with a relatively intact sellar floor was not identified with SFI.

Conclusion: This study highlights the potential of the CNN model for the efficient assessment of PA invasion.
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http://dx.doi.org/10.3389/fnins.2022.900519DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9289618PMC
July 2022

Combined Effect of Smoking and Fatty Liver Disease on the Progression of Type 2 Diabetes: Insights from a Population-Based Cohort Study.

Comput Math Methods Med 2022 9;2022:1776875. Epub 2022 Jul 9.

Department of Endocrinology, The Fifth Medical Center of Chinese PLA Hospital, Beijing, China.

Objectives: Fatty liver disease (FLD) is strongly linked to the occurrence of type 2 diabetes mellitus (T2DM). Insulin resistance (IR) is linked to smoking. Our study's purpose was to see how smoking and fatty liver accompanied affected the development of T2DM in the past.

Materials And Methods: We collected data from 15,464 Japanese adults aged 18 to 79 years who took part in the NAGALA research, and our team utilized a Cox proportion risk model to look at the combination effect of FLD and smoking status on the incidence of T2DM. Participants were separated into three categories: nonsmokers, ex-smokers, and current smokers. An abdominal ultrasound was used to diagnose FLD.

Results: 384 subjects had T2DM after a median follow-up of 5.4 years. In comparison to the other groups, current FLD smokers had a greater chance of developing T2DM. Ex-smokers and present FLD smokers, on the other hand, had no significant difference in their likelihood of acquiring T2DM. When compared to ex-smokers and nonsmokers without FLD, current smokers with FLD had a considerably greater chance of acquiring T2DM. Furthermore, the risk of T2DM among nonsmokers, ex-smokers with FLD, and current smokers without FLD was not statistically significant.

Conclusions: In order to prevent the progression of T2DM, we should recognize that smoking status may vary in FLD.
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http://dx.doi.org/10.1155/2022/1776875DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9288327PMC
July 2022

The risk factors for urinary incontinence in female adults with chronic cough.

BMC Pulm Med 2022 Jul 18;22(1):276. Epub 2022 Jul 18.

State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, National Center for Respiratory Medicine, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, 28 middle Qiaozhong Rd, Liwan District, Guangzhou, Guangdong, People's Republic of China.

Background: Female patients with chronic cough are more likely to suffer from urinary incontinence (UI). However, there are few data in regard of risks related with UI in female adults with chronic cough.

Method: We recruited female adult patients with chronic cough from the cough specialist clinic. Demographic information and clinical characteristics including age, BMI, duration of cough, severity of cough, nature and timing of cough, cough triggers, concomitant symptoms, comorbidities and UI condition were collected. The demographics and clinical features of patients with UI and those without UI were compared.

Result: A total of 700 female patients with the main symptom of chronic cough were included, of whom 351 (50.1%) presented with UI. As compared with patients without UI, patients with UI showed a longer mean age (years) (49.5 vs. 42.4, p < 0.001), a more severe cough symptom (median of cough Visual Analogue Scale: 65 vs. 50, p < 0.001), a higher prevalence of chronic sinusitis (17.6% vs. 8.6%, p = 0.002), and combined with a higher incidence of abdominal muscle pain due to cough (39.6% vs. 18.7%, p < 0.001).In addition, patients in UI group whose cough were more easily triggered by exercise (28.2% vs. 17.2%, p = 0.048). Multivariate logistic regression analysis indicated the above five variables were risk factors for UI in female adult patients with chronic cough.

Conclusion: Urinary incontinence is a common complication in female patients with chronic cough. Older age, severe cough, combing with a higher proportion of chronic sinusitis and abdominal muscle pain, a cough easily triggered by exercise are identified as risk factors for urinary incontinence. We should pay more attention to female chronic coughers with these risk factors in clinics.
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http://dx.doi.org/10.1186/s12890-022-02069-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9295435PMC
July 2022

Risk factors associated with augmented renal clearance in a mixed intensive care unit population: a retrospective study.

Int J Clin Pharm 2022 Jul 14. Epub 2022 Jul 14.

Faculté de Pharmacie de L'Université de Montréal, Pavillon Jean-Coutu 2940, Chemin de Polytechnique, Montreal, QC, H3T 1J4, Canada.

Background: Augmented renal clearance is increasingly recognized in critically ill patients. This condition may lead to suboptimal dosing of renally excreted medications.

Aim: Our primary objective was to identify demographic and clinical factors associated with augmented renal clearance in a mixed critically ill population.

Method: This retrospective single center observational cohort study evaluated patients admitted in a mixed adult intensive care unit for augmented renal clearance, defined as a creatinine clearance of ≥ 130 ml/min/1.73m through weekly 24-h urine collection. Variables associated with augmented renal clearance were identified using univariate analysis, then served as covariates in a backward stepwise logistic regression. Goodness-of-fit of the model was assessed and receiver operating characteristic curve was generated.

Results: Augmented renal clearance was observed in 25.3% of the study cohort (n = 324). Age below 50 years (adjusted odds ratio 7.32; 95% CI 4.03-13.29, p < 0.001), lower serum creatinine at intensive care admission (adjusted odds ratio 0.97; 95% CI 0.96-0.99, p < 0.001) and trauma admission (adjusted odds ratio 2.26; 95% CI 1.12-4.54, p = 0.022) were identified as independent risk factors. Our model showed acceptable discrimination in predicting augmented renal clearance (Area under receiver operating characteristic curve (0.810; 95% CI 0.756-0.864, p < 0.001)).

Conclusion: We identified age below 50 years, lower serum creatinine upon intensive care admission and trauma as independent risk factors for augmented renal clearance, consistent with the literature suggesting that patients with low serum creatinine upon admission could have a higher risk of developing augmented renal clearance.
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http://dx.doi.org/10.1007/s11096-022-01458-9DOI Listing
July 2022

A Randomized Controlled Dose-Escalation Study of LY06006, a Recombinant Humanized Monoclonal Antibody to RANKL, in Chinese Healthy Adults.

Front Pharmacol 2022 15;13:893166. Epub 2022 Jun 15.

Department of Pharmacy, Peking University People's Hospital, Beijing, China.

This study was conducted to explore the safety, tolerance, pharmacokinetics, pharmacodynamics, and immunogenicity of LY06006, a recombinant humanized monoclonal antibody to RANKL, when administrated subcutaneously in Chinese healthy adults. This was a randomized, double-blinded, placebo-controlled, single ascending dose study performed in 32 healthy Chinese adults, who were randomly assigned to receive a single injection dose of 18, 60, 120 mg study drug or placebo with a follow-up of 140-252 days. No deaths or drug-related serious adverse events occurred. LY06006 was rapidly absorbed in the 60 mg group with a T range of 120-480 h and serum LY06006 concentrations decreased slowly 11-13 days after dosing with a long mean (SD) half-life of 389.58 (63.44) h. The most frequent AEs were elevated serum parathyroid hormone (PTH) level (83.3%), hypocalcemia (54.2%), and hypophosphatemia (45.8%). None of the 32 subjects tested positive for anti-drug antibody during the trial. Single-dose subcutaneous administration of LY06006 was safe and well-tolerated in healthy Chinese adults. C showed linear pharmacokinetic characteristics in the dose range of 18-120 mg based on dose-exposure proportionality analysis.
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http://dx.doi.org/10.3389/fphar.2022.893166DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240259PMC
June 2022

Effects of residual monensin in livestock manure on nitrogen transformation and microbial community during "crop straw feeding-substrate fermentation-mushroom cultivation" recycling system.

Waste Manag 2022 Jul 30;149:333-344. Epub 2022 Jun 30.

Jilin Provincial Key Laboratory of Grassland Farming, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, Jilin, PR China. Electronic address:

Although crop-livestock integration recycling systems improve nitrogen (N) utilization in agroecosystems, there are limited studies regarding impacts of residual antibiotics in livestock manure on N transformation in entire recycling system. The objective was to evaluate effects of feeding monensin on N recycling during "straw feeding-substrate fermentation-mushroom cultivation". This experiment contained 3 steps. During straw feeding, beef cattle were allocated into 2 groups and fed diets with or without monensin, respectively. During fermentation, beef cattle manure (with or without monensin) and straw (corn or wheat) and were co-fermented for 35 d to produce substrates. During cultivation, Agaricus bisporus was cultivated on 4 substrates to recycle N in the form of mushrooms. Rates of N retention during fermentation were significant higher for monensin and corn straw treatments and there was an significant interaction between straw and antibiotic on N retention rate during cultivation. However, residual monensin significantly reduced amount of recycled N during entire recycling system, due to changes in N transformation-associated enzyme activity, ammonification and denitrification plus microbial community structure and succession. Specifically, residual monensin inhibited growth of dominant bacterial phylum Bacteroidetes and fungal phylum Neocallimastigomycota, and increased bacterial phylla Actinobacteriota and Firmicutes. These alterations in functional microbes increased N retention rates but reduced mushroom yields in antibiotic treatments during cultivation. In conclusion, monensin decreased the N recycling rate in recycling system, but also reduced N losses during fermentation by inhibiting ammonification and denitrification, so, avoiding antibiotics usage is an effective strategy to improve the efficiency of recycling systems.
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http://dx.doi.org/10.1016/j.wasman.2022.06.015DOI Listing
July 2022

Transfer of Nitrogen and Phosphorus From Cattle Manure to Soil and Oats Under Simulative Cattle Manure Deposition.

Front Microbiol 2022 14;13:916610. Epub 2022 Jun 14.

Jilin Provincial Laboratory of Grassland Farming, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, China.

Simulated cattle manure deposition was used to estimate nutrient transfer to soil and oats and to investigate changes in microbial community composition and functional groups in oat rhizospheres. Nutrient absorption and return efficiency were calculated as a series of standard calculation formulas, and total nutrient transfer efficiency was nutrient absorption efficiency plus nutrient return efficiency. In total, 74.83% of nitrogen (N) and 59.30% of phosphorus (P) in cattle manure were transferred to soil and oats, with 11.79% of N and 7.89% of P in cattle manure absorbed by oats, and the remainder sequestered in the soil for 80 days after sowing. Cattle manure increased oat root length, surface, and volume under 0.2 mm diameter, and improved relative abundance of the microbiome known to be beneficial. In response to cattle manure, several bacteria known to be beneficial, such as Proteobacteria, Bacteroidota, and Firmicutes at phyla the level and , , and at the genus level, were positively related to oat biomass and nutrient accumulation. For fungal communities, the relative abundance of Ascomycota is the predominant phylum, which varied in a larger range in the control treatment (81.0-63.3%) than the cattle manure deposition treatment (37.0-42.9%) as plant growing days extend. The relevant abundance of Basidiomycota known as decomposer was higher in cattle manure deposition treatment compared to that in control treatment at 15 days after sowing. More importantly, cattle manure deposition inhibited trophic mode within pathotroph like and fungal genus and promoted saprotroph and symbiotroph.
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http://dx.doi.org/10.3389/fmicb.2022.916610DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9238326PMC
June 2022

Reduction in RF Loss Based on AlGaN Back-Barrier Structure Changes.

Micromachines (Basel) 2022 May 26;13(6). Epub 2022 May 26.

The Engineering Research Center for Optoelectronics of Guangdong Province, School of Physics and Optoelectronics, South China University of Technology, Guangzhou 510640, China.

We designed a high electron mobility transistor (HEMT) epitaxial structure based on an AlGaN/GaN heterojunction, utilizing Silvaco TCAD, and selected AlGaN with an aluminum composition of 0.1 as the back-barrier of the AlGaN/GaN heterojunction. We enhanced the confinement of the two-dimensional electron gas (2DEG) by optimizing the structural parameters of the back barrier, so that the leakage current of the buffer layer is reduced. Through these optimization methods, a lower drain leakage current and a good radio frequency performance were obtained. The device has a cut-off frequency of 48.9 GHz, a maximum oscillation frequency of 73.20 GHz, and a radio frequency loss of 0.239 dB/mm (at 6 GHz). This work provides a basis for the preparation of radio frequency devices with excellent frequency characteristics and low RF loss.
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http://dx.doi.org/10.3390/mi13060830DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9227317PMC
May 2022

Effect of Sulfur Content in Sulfate-Rich Copper Tailings on the Properties of MgO-Activated Slag Materials.

Materials (Basel) 2022 Jun 20;15(12). Epub 2022 Jun 20.

School of Civil Engineering and Architecture, Anhui University of Science and Technology, Huainan 232001, China.

The high-value utilization of sulfate-rich tailings (SRCTs) can accelerate their mass consumption, so the many problems caused by the massive accumulation of SRCTs can be alleviated, such as environmental pollution, land occupation, security risk, etc. This study proposes using SRCTs to replace fine natural aggregates in MgO-activated slag materials (MASMs) and investigate the influence of the sulfur content in SRCTs on the properties of MASMs. The experimental results showed that the 28 d compressive strength of MASM mortars was increased by up to 83% using SRCT composites. Two major mechanisms were discovered: additional hydration product formation and pore structure refinement. The results of XRD suggested that incorporating SRCT composite into MASMs increased the production of expansive sulfate-containing hydration products, such as ettringite, gypsum, and hydroxyl-Afm. The results of element mapping showed that the oxidation of pyrite in SRCTs could release sulfates into the surrounding area and participate in the hydration of MASM, indicating that SRCTs can work as an auxiliary activator for MASMs. Furthermore, the addition of SRCT significantly refined the pore structure of MASMs, leading to the reduction in porosity by up to 37.77%. These findings confirm a synergistic effect on activating the slag between SRCTs and MgO, promoting the mass utilization of SRCTs. As a result, the additional expansive hydration products contribute to the enhanced compressive strength and refined pore structure.
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http://dx.doi.org/10.3390/ma15124340DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9254570PMC
June 2022

Non-Wilms' renal tumors in children: experience with 139 cases treated at a single center.

BMC Urol 2022 Jun 22;22(1):89. Epub 2022 Jun 22.

Department of Urology, National Children's Medical Center, Beijing Children's Hospital of Capital Medical University, No. 56 Nanlishi St, Xicheng District, Beijing, 100045, China.

Background: Pediatric non-Wilms renal tumors (NWRTs), which comprise a small proportion of renal tumors, are a heterogeneous group of neoplasms with variable malignant potential, mortality, and response to treatment. We performed this study to determine the clinical characteristics, management and prognosis of children with Pediatric NWRTs.

Methods: Medical records of all patients (n = 139) treated for NWRTs over a 12-year period (2008.01-2019.10) at a single center were reviewed retrospectively.

Results: The histopathological groups of NWRTs included malignant rhabdoid tumor of the kidney (MRTK) (n: 30, 21.6%), renal cell cancer (RCC) (n: 26,18.7%), clear cell sarcoma of the kidney (CCSK) (n: 24,17.3%), congenital mesoblastic nephroma (CMN) (n: 21,15.1%), cystic nephroma (CN) (n: 16,11.5%), metanephric tumors (n: 12, 8.6%), renal angiomyoliporma (RAML) (n: 3, 2.2%), renal primitive neuroectodermal tumor (n: 2, 1.4%), renal hemangioma (n: 2, 1.4%), inflammatory myofibroblastic tumor (n: 2, 1.4%), ossifying renal tumor of infancy (ORTI) (n: 1, 0.7%). The distribution of all malignant NWRTs, including MRTK, CCSK, RCC and PNET, according to stage was as follows: stages I (n = 26), II (n = 16), III (n = 29), and IV (n = 11). The summary table shows the treatment offered to children with NWRTs. A total of 123 children were followed up for an average of 42 months. Sixteen children were lost to follow-up. Tumor-free survival was observed in 94 children. One patient who suffered from RCC is currently receiving targeted therapy and survives with the tumor. Twenty-eight children (22.8%) died.

Conclusions: Pediatric NWRTs comprise 19.1% of all renal tumors in our single center. Most NWRTs can readily be distinguished using a range of immunohistochemical markers. Molecular genetic profiling has allowed much progress in the understanding of this group of tumors, making diagnosis and classification less difficult. The mainstay treatment of malignant NWRTs, including MRTK, CCSK, RCC and PNET, is comprehensive treatment. The mainstay treatment of benign NWRTs, including RAML, CN, ORTI, CMN, metanephric tumors, and renal hemangioma, is surgical resection alone and when the tumor diameter is smaller than 7 cm and the tumor locates in one pole, NSS can be performed.
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http://dx.doi.org/10.1186/s12894-022-01042-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9219123PMC
June 2022

Long-term drinking behavior change patterns and its association with hyperuricemia in chinese adults: evidence from China Health and Nutrition Survey.

BMC Public Health 2022 06 20;22(1):1230. Epub 2022 Jun 20.

Department of Nephrology, Zhongshan Hospital, Fudan University, No.180 Fenglin Road, 200032, Shanghai, Shanghai, China.

Background: We aimed to explore the association between long-term drinking behavior change patterns with hyperuricemia (HUA) in Chinese community adults.

Methods: This study was designed as a community-based unbalanced cohort study involving 4127 adults aged between 18 ~ 75 years, derived from the China Health and Nutrition Survey (CHNS) in 1997 and 2009. Drinking behavior change patterns were categorized into: never drinking, change to drinking, quitting drinking, and continued drinking. The alcoholism, type, and frequency of drinking were further categorized. We applied logistic regression models to explore the associations between drinking behavior change patterns and HUA.

Results: The average age of the participants was 54.6 (± 11.3) years and 47.8% were male. The overall prevalence of HUA was 15.5%. Drinking behavior change patterns of quitting (aOR 1.8; 95% CI 1.1 ~ 2.8) and continued drinking (aOR 2.0; 95% CI 1.3 ~ 3.0) were positively associated with high risks of HUA in the male participants. Early drinking behaviors such as liquor intake (aOR 1.8; 95% CI 1.4 ~ 2.5) and high consumption or frequency showed a positive correlation with HUA. Of note, heavy alcoholism (aOR 2.0; 95% CI 1.4 ~ 2.8) and daily drinking (aOR 2.5; 95% CI 1.7 ~ 3.6) had the highest risks of HUA. Furthermore, in the male participants, the association between early total alcohol intake and HUA was more pronounced at 18 standard drinks intake, with a stable increasing trend. In contrast, no statistical correlation was observed between the drinking behaviors and HUA in the female participants.

Conclusions: Drinking behavior change patterns of quitting and continued drinking are strongly associated with increased risks of HUA in males. The risks emanated from early drinking behaviors such as liquor drinking, high drinking frequency, and alcohol consumption. Although quitting drinking was associated with lower HUA risks compared to continued drinking, it still presented an undeniable risk for HUA.
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http://dx.doi.org/10.1186/s12889-022-13637-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9210654PMC
June 2022

Microscopic Transsphenoidal Resection of Giant Pituitary Adenomas: Analysis of the Factors Limiting the Degree of Resection in 73 Cases.

Front Neurol 2022 27;13:880732. Epub 2022 May 27.

Department of Neurosurgery, The 900th Hospital, Fuzong Clinical Medical College of Fujian Medical University, Fuzhou, China.

Purpose: To analyze the risk factors affecting the gross-total resection of giant pituitary adenomas using a transsphenoidal approach under a microscope to provide a reference basis for formulating an appropriate surgical strategy.

Methods: The clinical data of patients who underwent microscopic transsphenoidal resection of giant pituitary adenomas in a single center from January 2011 to December 2020 were retrospectively analyzed. Based on magnetic resonance imaging and surgical records, the predictive factors affecting the gross-total resection of giant pituitary adenomas under microscopy were determined through univariate and multivariate analyses.

Results: A total of 73 patients with giant pituitary adenomas underwent transsphenoidal microsurgery. Gross-total resection was performed in 19 cases (26%), subtotal resection in 31 cases (42%), partial resection in 21 cases (29%), and the degree of resection was <50% in only two cases (3%). After binary logistic analysis, it was found that it was more difficult to completely remove giant pituitary adenomas with a Knosp grade 3-4 [odds ratio (OR) = 0.214, 95% confidence interval (CI): 0.05-0.917; = 0.038], greater proportion of tumor suprasellar volume (odds ratio = 0.937, 95% confidence interval: 0.898-0.978; = 0.003), and intraoperative evidence of invasion of the cavernous sinus (odds ratio = 0.187, 95% CI: 0.039-0.898; = 0.036).

Conclusion: It is difficult to remove a giant pituitary adenoma invading the cavernous sinus completely with a higher degree of invasion of the suprasellar region using microscopic transsphenoidal surgery. The combined application of multiple surgical methods can help to improve the degree of resection during a single operation.
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http://dx.doi.org/10.3389/fneur.2022.880732DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9195514PMC
May 2022

Ethnicity evaluation of ferric pyrophosphate citrate among Asian and Non-Asian populations: a population pharmacokinetics analysis.

Eur J Clin Pharmacol 2022 Jun 17. Epub 2022 Jun 17.

Center for Drug Clinical Research, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Purpose: To evaluate the potential ethnic differences of ferric pyrophosphate citrate (FPC, Triferic) in healthy subjects and patients with hemodialysis-dependent stage 5 chronic kidney disease (CKD-5HD) and identify covariates that may influence pharmacokinetics (PK) of FPC.

Methods: Data were collected from 2 Asian and 4 non-Asian clinical studies involving healthy subjects and CKD-5HD patients. Three population PK models were developed: M1 for intravenous (IV) administration of FPC in healthy subjects; M2 for dialysate administration of FPC in CKD-5HD patients; M3 for pre-dialyzer administration of FPC in CKD-5HD patients. All the models were fitted to concentration versus time data of FPC using the nonlinear mixed effect approach with the NONMEM program. All statistical analyses were performed using SAS version 9.4.

Results: In total, 26 Asians and 65 non-Asians were included in the final model analysis database. Forty healthy subjects were administered FPC via intravenous (IV) route and 51 patients with CKD-5HD via dialysate (N = 50) and pre-dialyzer blood circuit administration (N = 51). The PK parameters of FPC IV were similar. The population PK model showed good parameter precision and reliability as shown by model evaluation, and no relevant influence of ethnicity on PK parameters was observed. In healthy subjects, the maximum observed plasma concentration (C) and area under the plasma concentration-time curve (AUC) decreased with increase in lean body mass (LBM) and the average serum total iron at 6 h before the baseline period (Fe), whereas, in both patient populations, C and AUC decreased with increase in LBM and decrease in Fe. Other factors such as gender, age, Fe, and ethnicity had no influence on PK exposures in patients. The influence of LBM on PK exposures in patients was smaller than that in healthy subjects (ratio of AUC for the 5th [68 kg] and 95th [45 kg] patient's LBM was almost 1). The influence of Fe and LBM on PK exposures was < 50%.

Conclusion: The population pharmacokinetics model successfully described the PK parameters of FPC in healthy subjects and CKD-5HD patients and were comparable between Asian and non-Asian populations.
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http://dx.doi.org/10.1007/s00228-022-03328-9DOI Listing
June 2022

Prevalence and Molecular Identification of spp. in Non-human Primates in a Zoological Garden in Nanjing, China.

Front Vet Sci 2022 30;9:906822. Epub 2022 May 30.

MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.

Objective: spp. are globally distributed zoonotic parasites that infect various hosts, among which non-human primates (NHPs) have been identified as one of the most common hosts of these parasites. Consequently, the infections of spp. in captive NHPs from Nanjing Hongshan Forest Zoo in China were investigated in order to assess their zoonotic potential.

Methods: A total of 120 fresh fecal samples, including 19 species of NHPs, were collected from four breeding bases of the zoo from May to June 2019. The infections of six species of spp. were detected by PCR using the 16S or 18S rDNA-specific primers, and the positive samples were sequenced and analyzed.

Results: spp. were detected as positive in 59 NHPs fecal samples (49.17%), including five species: (7.50%), (22.50%), (22.50%), (10.00%) and (1.67%). Infection with one species was more common (35%) than co-infections (13.33%) or infections with three species (0.83%). There was a significantly higher prevalence rate of spp. in the species and than in sp., , and .

Conclusion: spp. are highly prevalent in the NHPs raised in Nanjing Hongshan Forest Zoo. Therefore, attention should be paid to the development of containment strategies of spp. in this zoological garden.
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http://dx.doi.org/10.3389/fvets.2022.906822DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9189403PMC
May 2022

Design and optimization of wall-climbing robot impeller by genetic algorithm based on computational fluid dynamics and kriging model.

Sci Rep 2022 06 10;12(1):9571. Epub 2022 Jun 10.

School of Mechanical and Aerospace Engineering, Jilin University, Changchun, 130025, China.

In recent years, wall-climbing robots have begun to replace manual work at heights to reduce economic losses and casualties caused by working at heights. This paper designs a negative pressure adsorption type wall-climbing robot and analyzes the internal fluid movement state of its negative pressure device and the force analysis of the robot when it is adsorbed and balanced. Furthermore, through the experimental prototype, the influence of wall material, robot pose, negative pressure cavity shape and sealing method on the adsorption performance of the wall-climbing robot is explored. The computational fluid dynamics simulation (CFD) simulation method and experimental results are used to verify each other, which proves the correctness of the simulation results. Based on the Kriging surrogate model, the functional relationship between the impeller blade outlet angle, the impeller inlet diameter, the number of blades as the design variables, the negative pressure as the dependent variable was established, and the genetic algorithm (GA) was used to optimize it. Compared with the original design, the optimized design results of impeller parameters have increased the negative pressure value from 3534.75 to 4491.19 Pa, an increase of 27.06%.
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http://dx.doi.org/10.1038/s41598-022-13784-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9187699PMC
June 2022

Bio-Based Admixture (Black Tea Extraction) for Better Performance of Metakaolin Blended Cement Mortars.

Materials (Basel) 2022 Jun 3;15(11). Epub 2022 Jun 3.

Department of Civil, Construction, and Environmental Engineering, The University of Alabama, Tuscaloosa, AL 35487, USA.

With high pozzolanic reactivity, metakaolin (MK) is a popular supplementary cementitious material (SCM), which can be used to partially replace Portland cement in concretes. Due to its small particle size, however, MK can agglomerate, resulting in a nonuniform matrix and underperformance of the produced concrete. To address this issue, this paper exploits a low-cost, bio-based admixture-black tea extract (BTE)-to replace the traditional petroleum-based chemical admixture to enhance the dispersion and workability of MK blended cement mortars. Major biomolecules in the BTE such as caffeine, catechin, theanine, and theaflavin are rich in polyphenol, hydroxyl, and carboxylic acid groups, which can interact with cement particles and have profound effects on the hydration process and microstructure of the hydration products. Experimental studies showed that BTE does improve the workability of the MK blended cement mortar. More importantly, the BTE introduces significant change on the microstructure of the hardened pastes. Both the pores with size less than 50 nm and the total porosity of the hardened paste were significantly reduced, leading to a significant improvement in the micro- and macro-mechanical properties of the hardened paste. Experimental results suggest that up to 35% greater improvement in the compressive strength at 28 days was achieved using the proposed bio-admixture. Economic and environmental advantages of using the BTE as a renewable admixture were also illustrated through analyzing the cost-benefit, embodied carbon, and eco-efficiency of the MK blended mortars.
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http://dx.doi.org/10.3390/ma15113994DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9181937PMC
June 2022

PD-L1 Protein Expression Is Associated With Good Clinical Outcomes and Nomogram for Prediction of Disease Free Survival and Overall Survival in Breast Cancer Patients Received Neoadjuvant Chemotherapy.

Front Immunol 2022 20;13:849468. Epub 2022 May 20.

Department of Breast Surgical Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Objective: This study aims to investigate the potential prognostic significance of programmed death ligand-1 (PD-L1) protein expression in tumor cells of breast cancer patients received neoadjuvant chemotherapy (NACT).

Methods: Using semiquantitative immunohistochemistry, the PD-L1 protein expression in breast cancer tissues was analyzed. The correlations between PD-L1 protein expression and clinicopathologic characteristics were analyzed using Chi-square test or Fisher's exact test. The survival curve was stemmed from Kaplan-Meier assay, and the log-rank test was used to compare survival distributions against individual index levels. Univariate and multivariate Cox proportional hazards regression models were accessed to analyze the associations between PD-L1 protein expression and survival outcomes. A predictive nomogram model was constructed in accordance with the results of multivariate Cox model. Calibration analyses and decision curve analyses (DCA) were performed for the calibration of the nomogram model, and subsequently adopted to assess the accuracy and benefits of the nomogram model.

Results: A total of 104 breast cancer patients received NACT were enrolled into this study. According to semiquantitative scoring for IHC, patients were divided into: low PD-L1 group (61 cases) and high PD-L1 group (43 cases). Patients with high PD-L1 protein expression were associated with longer disease free survival (DFS) (mean: 48.21 months vs. 31.16 months; P=0.011) and overall survival (OS) (mean: 83.18 months vs. 63.31 months; P=0.019) than those with low PD-L1 protein expression. Univariate and multivariate analyses indicated that PD-L1, duration of neoadjuvant therapy, E-Cadherin, targeted therapy were the independent prognostic factors for patients' DFS and OS. Nomogram based on these independent prognostic factors was used to evaluate the DFS and OS time. The calibration plots shown PD-L1 based nomogram predictions were basically consistent with actual observations for assessments of 1-, 3-, and 5-year DFS and OS time. The DCA curves indicated the PD-L1 based nomogram had better predictive clinical applications regarding prognostic assessments of 3- and 5-year DFS and OS, respectively.

Conclusion: High PD-L1 protein expression was associated with significantly better prognoses and longer DFS and OS in breast cancer patients. Furthermore, PD-L1 protein expression was found to be a significant prognostic factor for patients who received NACT.
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http://dx.doi.org/10.3389/fimmu.2022.849468DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9163312PMC
May 2022

The Double-Faceted Role of Leucine-Rich Repeat Kinase 2 in the Immunopathogenesis of Parkinson's Disease.

Front Aging Neurosci 2022 11;14:909303. Epub 2022 May 11.

Department of Geriatrics, Xiangya Hospital, Central South University, Changsha, China.

() is one of the most common causative genes in Parkinson's disease (PD). The complex structure of this multiple domains' protein determines its versatile functions in multiple physiological processes, including migration, autophagy, phagocytosis, and mitochondrial function, among others. Mounting studies have also demonstrated the role of LRRK2 in mediating neuroinflammation, the prominent hallmark of PD, and intricate functions in immune cells, such as microglia, macrophages, and astrocytes. Of those, microglia were extensively studied in PD, which serves as the resident immune cell of the central nervous system that is rapidly activated upon neuronal injury and pathogenic insult. Moreover, the activation and function of immune cells can be achieved by modulating their intracellular metabolic profiles, in which LRRK2 plays an emerging role. Here, we provide an updated review focusing on the double-faceted role of LRRK2 in regulating various cellular physiology and immune functions especially in microglia. Moreover, we will summarize the latest discovery of the three-dimensional structure of LRRK2, as well as the function and dysfunction of LRRK2 in immune cell-related pathways.
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http://dx.doi.org/10.3389/fnagi.2022.909303DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9131027PMC
May 2022

Validation of the Augmented Renal Clearance in Trauma Intensive Care scoring system for augmented renal clearance prediction in a trauma subgroup of a mixed ICU population.

J Clin Pharm Ther 2022 May 28. Epub 2022 May 28.

Faculté de pharmacie de l'Université de Montréal, Montreal, Quebec, Canada.

What Is Known And Objective: Augmented renal clearance is prevalent in trauma patients and leads to subtherapeutic levels of renally eliminated medications with potentially unfavourable clinical outcomes. The Augmented Renal Clearance of Trauma in Intensive Care (ARCTIC) score has been developed to predict augmented renal clearance in critically ill trauma patients. Our primary objective was to validate this score among the trauma subgroup of a mixed intensive care patient cohort.

Methods: This single-centre, retrospective, observational cohort study assessed augmented renal clearance using a timed 24-h urine collection performed weekly. ARC was defined as a measured creatinine clearance of ≥130 ml/min/1.73 m . ARCTIC score performance was evaluated through a receiver operator characteristic curves and analysis of sensitivities and specificities for the trauma subgroup, the medical/surgical subgroup and the pooled cohort.

Results And Discussion: Augmented renal clearance was observed in 33.9% (n = 58) of trauma patients (n = 171) and 15.7% (n = 24) of medical/surgical patients (n = 153). Examination of different cutoffs for the ARCTIC score in our trauma population confirmed that the optimal cutoff score was ≥6. Comparison between ROC curves for ARCTIC score and for regression model based upon our data in trauma patients indicated validation of the score in this subgroup. Comparison of sensitivities and specificities for ARCTIC score between trauma (93.1% and 41.6%, respectively) and medical/surgical subjects (87.5% and 49.6%, respectively) showed no clinical nor statistical difference, suggesting validation for the medical/surgical subgroup as well.

What Is New And Conclusion: In our mixed ICU population, the ARCTIC score was validated in the trauma subgroup. We also found that the score performed well in the medical/surgical population. Future studies should assess the performance of the ARCTIC score prospectively.
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http://dx.doi.org/10.1111/jcpt.13695DOI Listing
May 2022

MicroRNA-382 Promotes M2-Like Macrophage the SIRP-α/STAT3 Signaling Pathway in Aristolochic Acid-Induced Renal Fibrosis.

Front Immunol 2022 2;13:864984. Epub 2022 May 2.

Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China.

Aristolochic acid nephropathy (AAN) is a type of drug-induced nephropathy and is correlated with a potentially progression of kidney fibrosis. However, whether miR-382 is implicated in macrophage activation in AA-induced kidney fibrosis remains elusive. Here, cell-sorting experiments defined a significant miR-382 enrichment in renal macrophage after AAN 14 days. Then, we found that treatment of AA induced a significant switch in the phenotype of macrophage both and . Furthermore, miR-382 knockout (KO) mice and miR-382 bone marrow-derived macrophage (BMDM) were subjected to AA induction. We found that both systemic KO and macrophage-specific miR-382 depletion notably suppressed M2-like macrophage activation as well as kidney interstitial fibrosis. Additionally, adoptive transfer of miR-382 overexpression BMDMs into mice promoted AA-induced kidney injury. Moreover, in cultured macrophage, upregulation of miR-382 promoted M2-related gene expression, accompanied by downregulation of signal regulatory protein α (SIRP-α) and activation of signal transducer and activator of transcription 3 (STAT3). The interaction between miR-382 and SIRP-α was evaluated dual-luciferase assay. Knockdown of SIRP-α upregulated phosphorylated STAT3 at S727 and Y705. Pharmacological inhibition of STAT3 was performed both and . Inhibition of STAT3 attenuated AA-induced kidney fibrosis, in parallel to lesser macrophage M2 polarization. Coculture experiments further confirmed that overexpressed miR-382 in macrophage promoted injuries of tubular cells. Luminex bio-chip detection suggested that IL-4 and CCL-5 were critical in the cross talk between macrophages and tubular cells. Taken together, our data suggest that miR-382 is a critical mediator in M2-like macrophage polarization and can be a promising therapeutic target for kidney fibrosis.
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http://dx.doi.org/10.3389/fimmu.2022.864984DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9108237PMC
May 2022

Self-Supporting, Binder-Free, and Flexible TiCT MXene-Based Supercapacitor Electrode with Improved Electrochemical Performance.

ACS Nano 2022 May 18. Epub 2022 May 18.

School of Materials Science and Engineering, Sun Yat-sen University, Guangzhou 510275, P. R. China.

MXenes have shown great potential for supercapacitor electrodes due to their unique characteristics, but simultaneously achieving high capacitance, rate capability, and cyclic stability along with good mechanical flexibility is exceptionally challenging. Here, highly enhanced capacitance, rate capability, and cyclic stability, as well as good mechanical flexibility for TCT MXene-based supercapacitor electrodes are simultaneously obtained by engineering the electrode structure, modifying the surface chemistry, and optimizing the fabrication process an optimized integration approach. This approach combines and more importantly optimizes three methods that all require a calcination process: carbonizing grown polymer ("C") on the MXene, alkali treatment ("A"), and template sacrificing ("P"); and the optimized processes lead to more abundant active sites, faster ion accessibility, better chemical stability, and good mechanical flexibility. The obtained P-MXene/C-A electrodes are binder-free and self-supporting and not only have good mechanical flexibility but also demonstrate much larger capacitances and better rate performance than the pristine MXene electrode. Specifically, the P-MXene/C-A electrode (PAQ: quinone-amine polymer) achieves a high capacitance of 532.9 F g at 5 mV s, together with superior rate performance and improved cyclic stability (97.1% capacitance retention after 40 000 cycles at 20 A g) compared with the pristine MXene (79.6% retention) and P-MXene-A (77.3% retention) electrodes. In addition, it is discovered that carbonizing grown polymers can variously remove the -F group and the removal effect can be accumulated with that by the alkali treatment.
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http://dx.doi.org/10.1021/acsnano.2c03351DOI Listing
May 2022

Energy restriction induced SIRT6 inhibits microglia activation and promotes angiogenesis in cerebral ischemia via transcriptional inhibition of TXNIP.

Cell Death Dis 2022 May 11;13(5):449. Epub 2022 May 11.

Department of Neurology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan Province, P.R. China.

Energy restriction (ER) protects against cerebral ischemic injury, but the underlying mechanism remains largely unclear. Here, rats were fed ad libitum (AL) or on an alternate-day food deprivation intermittent fasting (IF) diet for 3 months, followed by middle cerebral artery occlusion (MCAO) surgery. The body weight, infarct volume, and neurological deficit score were accessed at the designated time points. ELISA, qRT-PCR, and Western blotting were used to determine cytokine secretion and the expression of SIRT6, TXNIP, and signaling molecules, respectively. Immunofluorescence evaluated microglial activation and angiogenesis in vivo. For in vitro study, oxygen-glucose deprivation/reoxygenation (OGD/R)-treated cell model was generated. MTT and tube formation assays were employed to determine cell viability and tube formation capability. ChIP assay detected chromatin occupancy of SIRT6 and SIRT6-mediated H3 deacetylation. We found that IF or ER mimetics ameliorated cerebral ischemic brain damage and microglial activation, and potentiated angiogenesis in vivo. ER mimetics or SIRT6 overexpression alleviated cerebral ischemia and reperfusion (I/R)-induced injury in vitro. SIRT6 suppressed TXNIP via deacetylation of H3K9ac and H3K56ac in HAPI cells and BMVECs. Downregulation of SIRT6 reversed ER mimetics-mediated protection during cerebral I/R in vitro. Our study demonstrated that ER-mediated upregulation of SIRT6 inhibited microglia activation and potentiated angiogenesis in cerebral ischemia via suppressing TXNIP.
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http://dx.doi.org/10.1038/s41419-022-04866-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9095711PMC
May 2022
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