Publications by authors named "Ye Zhang"

1,916 Publications

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A Response to: Letter to the Editor Regarding Preemptive Intravenous Nalbuphine for the Treatment of Post-Operative Visceral Pain: A Multicenter, Double-Blind, Placebo-Controlled, Randomized Clinical Trial.

Pain Ther 2022 Aug 10. Epub 2022 Aug 10.

Department of Anaesthesiology and Perioperative Medicine, The Second Hospital of Anhui Medical University, 678 Furong Road, Hefei, Anhui Province, China.

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http://dx.doi.org/10.1007/s40122-022-00413-wDOI Listing
August 2022

Facet-selective etching trajectories of individual semiconductor nanocrystals.

Sci Adv 2022 Aug 10;8(32):eabq1700. Epub 2022 Aug 10.

Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA.

The size and shape of semiconductor nanocrystals govern their optical and electronic properties. Liquid cell transmission electron microscopy (LCTEM) is an emerging tool that can directly visualize nanoscale chemical transformations and therefore inform the precise synthesis of nanostructures with desired functions. However, it remains difficult to controllably investigate the reactions of semiconductor nanocrystals with LCTEM, because of the highly reactive environment formed by radiolysis of liquid. Here, we harness the radiolysis processes and report the single-particle etching trajectories of prototypical semiconductor nanomaterials with well-defined crystalline facets. Lead selenide nanocubes represent an isotropic structure that retains the cubic shape during etching via a layer-by-layer mechanism. The anisotropic arrow-shaped cadmium selenide nanorods have polar facets terminated by either cadmium or selenium atoms, and the transformation trajectory is driven by etching the selenium-terminated facets. LCTEM trajectories reveal how nanoscale shape transformations of semiconductors are governed by the reactivity of specific facets in liquid environments.
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http://dx.doi.org/10.1126/sciadv.abq1700DOI Listing
August 2022

Establishment of fingerprint and mechanism of anti-myocardial ischemic effect of Syringa pinnatifolia.

Biomed Chromatogr 2022 Aug 10. Epub 2022 Aug 10.

College of Pharmacy, Inner Mongolia Medical University, Hohhot, Inner Mongolia, PR China.

Objective: To establish the fingerprint of Syringa pinnatifolia Hemsl. (SP), analyze the blood components of SP, and explore the possible mechanism of SP's anti-myocardial ischemia, so as to provide scientific basis for the follow-up development and research of SP and lay a foundation for its clinical application.

Methods: The fingerprint of SP was established by UPLC-QE-MS and GC-MS. A rat Myocardial infarction (MI) was constructed by ligating the left anterior descending branch (LAD) of the rat coronary artery, and SP alcohol extract was administered to evaluate its anti-myocardial ischemic effect. We analyzed the blood components of SP, screened the active compounds, established a database of SP anti-myocardial ischemic targets, and explored the possible mechanism of SP in treating MI by bioinformatics. The rats were examined by echocardiography, serum biomarkers were determined, and pathological changes were observed by histopathological examination. TUNEL staining was performed to detect the apoptotic level of cells, and western blot and qRT-PCR were performed to detect the expression levels of Bcl-2, Bax and caspase-3 in heart tissues.

Results: In the fingerprint of SP, 24 common peaks were established, and the similarity evaluation results of 10 batches of SP were all > 0.9. UPLC-QE-MS and GC-MS detected a total of 17 active ingredients in the drug-containing serum, including terpenoids, flavonoids, phenols, phenylpropanoids and phenolic acids, the most abundant of which was resveratrol. Enrichment analysis of SP targets against myocardial ischemia revealed that key candidate targets of SP were significantly enriched in multiple pathways associated with apoptosis. Resveratrol was administered to the successfully modeled rats, and the results showed that the resveratrol group significantly reduced LVEDd and LVEDs and significantly increased EF and FS in all groups compared with the model group. Resveratrol significantly reduced the levels of CK-MB and LDH in serum compared to the model group (p < 0.001). Hematoxylin-eosin (HE) staining of rat myocardial tissue showed that all lesions were reduced under microscopic observation in the resveratrol group compared with the model group. RT-PCR and western blot results showed that resveratrol group down-regulated the expression of the pro-apoptotic factor Bax, up-regulated the expression of the anti-apoptotic factor Bcl-2, and decreased the expression of Caspase-3.

Conclusion: The established fingerprints are accurate, reliable and reproducible, and can be used as an effective method for the quality control of the herbs. The anti-myocardial ischemia effect of SP may be that resveratrol can improve cardiac function and inhibit cardiomyocyte apoptosis to protect cardiomyocytes. The present study provides ample evidence for the clinical use of SP, suggesting that this drug has great potential in the treatment of ischemic heart disease.
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http://dx.doi.org/10.1002/bmc.5475DOI Listing
August 2022

Vaginal microbiota and personal risk factors associated with HPV status conversion-A new approach to reduce the risk of cervical cancer?

PLoS One 2022 9;17(8):e0270521. Epub 2022 Aug 9.

BGI Genomics, BGI-Shenzhen, Shenzhen, China.

Vaginal microbiota (VMB) is associated with changes in Human papilloma virus (HPV) status, which consequently influences the risk of cervical cancer. This association was often confounded by personal risk factors. This pilot research aimed to explore the relationship between vaginal microbiota, personal risk factors and their interactions with HPV status conversion to identify the vaginal microbiota that was associated with HPV clearance under heterogeneous personal risk factors. A total of 38 women participated by self-collecting a cervicovaginal mucus (CVM) sample that was sent for metagenomics sequencing. Most of the participants also filled in personal risk factors questionnaire through an eHealth platform and authorized the use of their previous HPV genotyping results stored in this eHealth platform. Based on the two HPV results, the participants were grouped into three cohorts, namely HPV negative, HPV persistent infection, and HPV status conversion. The relative abundance of VMB and personal factors were compared among these three cohorts. A correlation investigation was performed between VMB and the significant personal factors to characterize a robustness of the panel for HPV status change using R programming. At baseline, 12 participants were HPV-negative, and 22 were HPV-positive. Within one year, 18 women remained HPV-positive, 12 were HPV-negative and 4 participants showed HPV clearance. The factors in the eHealth questionnaire were systematically evaluated which identified several factors significantly associated with persistent HPV infection, including age, salary, history of reproductive tract infection, and the total number of sexual partners. Concurrent vaginal microbiome samples suggest that a candidate biomarker panel consisting of Lactobacillus gasseri, Streptococcus agalactiae, and Timona prevotella bacteria, which may be associated with HPV clearance. This pilot study indicates a stable HPV status-related vaginal microbe environment. To establish a robust biomarker panel for clinical use, larger cohorts will be recruited into follow-up studies.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0270521PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9362946PMC
August 2022

Diagnosis of fetal total anomalous pulmonary venous connection based on the post-left atrium space ratio using artificial intelligence.

Prenat Diagn 2022 Aug 8. Epub 2022 Aug 8.

Echocardiography Medical Center, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.

Objective: To explore whether the post-left atrium space (PLAS) ratio would be useful for prenatal diagnosis of total anomalous pulmonary venous connection (TAPVC) using echocardiography and artificial intelligence.

Methods: We retrospectively included 642 frames of four-chamber view from 319 fetuses (32 with TAPVC and 287 without TAPVC) in end-systolic and end-diastolic periods with multiple apex directions. The average gestational age was 25.6±2.7 weeks. No other cardiac or extracardiac malformations were observed. The dataset was divided into a training set (n=540; 48 with TAPVC and 492 without TAPVC) and test set (n=102; 20 with TAPVC and 82 without TAPVC). The PLAS ratio was defined as the ratio of the epicardium-descending aortic distance to the center of the heart-descending aortic distance. Supervised learning was used in DeepLabv3+, FastFCN, PSPNet, and DenseASPP segmentation models. The area under the curve (AUC) was used on the test set.

Results: Expert annotations showed that this ratio was not related to the period or apex direction. It was higher in the TAPVC group than in the control group detected by expert and the four models. The AUC of expert annotations, DeepLabv3+, FastFCN, PSPNet, and DenseASPP were 0.977, 0.941, 0.925, 0.856, and 0.887, respectively.

Conclusion: Segmentation models achieve good diagnostic accuracy for TAPVC based on the PLAS ratio. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/pd.6220DOI Listing
August 2022

A highly sensitive and versatile fluorescent biosensor for pathogen nucleic acid detection based on toehold-mediated strand displacement initiated primer exchange reaction.

Anal Chim Acta 2022 Aug 25;1221:340125. Epub 2022 Jun 25.

Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, PR China; Guangdong Engineering and Technology Research Center for Rapid Diagnostic Biosensors, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, PR China. Electronic address:

Existing detection methods for pathogen nucleic acid detection, such as polymerase chain reaction (PCR), are complicated and expensive to perform. Here, we report a simple and versatile strategy for highly sensitive detection of pathogen nucleic acid based on toehold-mediated strand displacement initiated primer exchange amplification (t-PER). In the presence of the target, the blocked hairpin substrate is released by toehold-mediated strand displacement, which triggers the primer exchange reaction amplification. Then, multiple long tandem-repeat single-strands generated by PER open the molecular beacon to recover the fluorescence signal. The t-PER protocol also successfully directly detected human papilloma virus from clinical cervical swab samples, with consistent results compared to real time-polymerase chain reaction (RT-PCR). Moreover, the versatility and clinical feasibility of this method was further confirmed by measuring Epstein-Barr virus, hepatitis B virus, and Ureaplasma urealyticum from different clinical samples (serum samples and urine samples). This simple platform enabled specific and sensitive detection of pathogen nucleic acid in a format that might hold great potential for point-of-care infection diagnosis.
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http://dx.doi.org/10.1016/j.aca.2022.340125DOI Listing
August 2022

Electrospun tannin-rich nanofibrous solid-state membrane for wastewater environmental monitoring and remediation.

Chemosphere 2022 Aug 3:135810. Epub 2022 Aug 3.

School of Nano-Tech and Nano-Bionics, University of Science and Technology of China (USTC), Hefei, 230026, PR China; Suzhou Key Laboratory of Functional Molecular Imaging Technology, CAS Key Laboratory for Nano-Bio Interface, Suzhou Institute of Nano-Tech and Nano-Bionics (SINANO), Chinese Academy of Sciences, Suzhou, 215123, PR China. Electronic address:

Heavy metal, organic dyes, and bacterial contamination in water endanger human/animals' health, and therefore, the detection, adsorption, and capturing of contaminants are essential for environmental safety. Ligand-rich membranes are promising for sensors, adsorption, and bacterial decontamination. Herein, tannin (TA)-reinforced 3-aminopropyltriethoxysilane (APTES) crosslinked polycaprolactone (PCL) based nanofibrous membrane (PCL-TA-APTES) was fabricated via electrospinning. PCL-TA-APTES nanofibers possess superior thermal, mechanical, structural, chemical, and aqueous stability properties than the un-crosslinked membrane. It changed its color from yellowish to black in response to Fe ions due to supramolecular iron-tannin network (FeTA) interaction. Such selective sensing has been noticed after adsorption-desorption cycles. Fe concentration, solution pH, contact time, and ligand concentration influence FeTA coordination. Under optimized conditions followed by image processing, the introduced membrane showed a colorimetric linear relationship against Fe ions (16.58 μM-650 μM) with a limit of detection of 5.47 μM. The PCL-FeTA-APTES membrane could restrain phenolic group oxidation and result in a partial water-insoluble network. The adsorption filtration results showed that the PCL-FeTA-APTES membrane can be reused and had a higher methylene blue adsorption (32.04 mg/g) than the PCL-TA-APTES membrane (14.96 mg/g). The high capture efficiency of nanocomposite against Fe-based S. aureus suspension than Fe-free suspension demonstrated that Fe-bounded bacterium adhered to the nanocomposite through Fe/TA-dependent biointerface interactions. Overall, high surface area, rich phenolic ligand, porous microstructure, and super-wetting properties expedite FeTA coordination in the nanocomposite, crucial for Fe ions sensing, methylene blue adsorption-filtration, and capturing of Fe-bounded bacterium. These multifunctional properties could promise nanocomposite membrane practicability in wastewater and environmental protection.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135810DOI Listing
August 2022

Complications and oncological outcomes after salvage surgery for recurrent and residual hypopharyngeal squamous cell carcinoma: a retrospective cohort study.

Ann Transl Med 2022 May;10(9):525

Department of Head and Neck Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: Salvage surgery for hypopharyngeal squamous cell carcinoma (HPSCC) after radiotherapy may result in several postoperative complications and the oncological outcome is unsatisfying. Therefore, identifying the risk factors for postoperative complications and oncological outcome after salvage surgery is important. This study aimed to determine which HPSCC patients might benefit from salvage surgery following previous radiotherapy.

Methods: We retrospectively analyzed 91 HPSCC patients who underwent salvage surgery due to locoregional recurrence/residual disease after radiotherapy. The pre- and intraoperative characteristics with complications and oncological outcomes were collected through medical records and telephone follow-up. Risk factors for complications were analyzed by binary logistic regression. The oncological outcomes were assessed by overall survival (OS) after salvage surgery. Kaplan-Meier curves and Cox proportional hazard regression analysis were used for univariate and multivariate survival analyses.

Results: Postoperative complications occurred in 40.7% of patients, with pharyngo-cutaneous fistula (PCF) occurring in 29.7% of patients. Salvage surgery for local disease was the only independent risk factor for postoperative complications and PCF [complications: odds ratio (OR) =5.298, 95% confidence interval (CI): 1.163-24.130, P=0.031; PCF: OR =4.543, 95% CI: 1.187-17.387, P=0.027). In the subgroup of patients with local disease, time of curative treatment initiation >90 days (OR =7.331, 95% CI: 1.278-42.054, P=0.025) and preoperative hemoglobin <118 g/L (OR =10.101, 95% CI: 1.026-99.492, P=0.045) were independent risk factors for postoperative complications, while free flap reconstruction was an independent protective factor for PCF (OR =0.099, 95% CI: 0.010-0.934, P=0.043). The median OS time was 17 months, with 5-year OS rates of 30%. Age at salvage surgery <50 years [hazard ratio (HR) =2.047, 95% CI: 1.217-3.443, P=0.007] and recurrence or retreatment clinical T stage 3-4 (rcT3-4) (HR =2.051, 95% CI: 1.219-3.450, P=0.007) were identified as risk factors for OS. The 5-year OS rates of patients without and with both risk factors were 43% and 10% (P=0.001).

Conclusions: Salvage surgery for locoregional recurrence/residual disease after previous radiotherapy could improve survival in selected patients with HPSCC. Patients with local recurrence/residual disease had a higher complication rate. Efforts can be made to shorten the time of curative treatment initiation and treat anemia to reduce the risk of postoperative complications in this subgroup.
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http://dx.doi.org/10.21037/atm-22-1844DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9347053PMC
May 2022

Carbon Nanotubes/Polydopamine/ZSM-5 Composite Soil Conditioner with Good Controlled Release and Adsorption Properties.

Langmuir 2022 Aug 4. Epub 2022 Aug 4.

Jiangsu LuHong Landscaping Engineering Company Limited, Jiangsu 226100, P. R. China.

Currently, the excessive application of fertilizers and the random discharge of waste water, waste gas, and residues have led to more and more serious soil pollution problems. Zeolite is the most promising material for preparing a green and environmentally friendly soil conditioner. Herein, the carbon nanotubes/polydopamine/ZSM-5 composite soil conditioner was prepared by a facile two-step method, and it was used to release fulvic acid and adsorb methylene blue to improve the environment. The cumulative release rate of the composite soil conditioner was 52% within 430 h for fulvic acid, which had a good sustained release effect and could be sustained-released in different acid-based surroundings. In addition, it showed a good adsorption capacity of methylene blue, and it is about 80.02 mg/g which was about six times higher than that of ZSM-5. It was beneficial for the adsorption of methylene blue in a neutral environment. Finally, it could promote the growth of brassica chinensis and maize, and the promotion effect was 60 and 35%, respectively. Therefore, the carbon nanotubes/polydopamine/ZSM-5 composite soil conditioner is a green and efficient material, which provides a new strategy to solve the problem of soil pollution.
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http://dx.doi.org/10.1021/acs.langmuir.2c01175DOI Listing
August 2022

Multiple Machine Learning Methods Reveal Key Biomarkers of Obstructive Sleep Apnea and Continuous Positive Airway Pressure Treatment.

Front Genet 2022 13;13:927545. Epub 2022 Jul 13.

Sleep Medicine Center, Department of Respiratory and Critical Care Medicine, Mental Health Center, West China Hospital, Sichuan University, Chengdu, China.

Obstructive sleep apnea (OSA) is a worldwide health issue that affects more than 400 million people. Given the limitations inherent in the current conventional diagnosis of OSA based on symptoms report, novel diagnostic approaches are required to complement existing techniques. Recent advances in gene sequencing technology have made it possible to identify a greater number of genes linked to OSA. We identified key genes in OSA and CPAP treatment by screening differentially expressed genes (DEGs) using the Gene Expression Omnibus (GEO) database and employing machine learning algorithms. None of these genes had previously been implicated in OSA. Moreover, a new diagnostic model of OSA was developed, and its diagnostic accuracy was verified in independent datasets. By performing Single Sample Gene Set Enrichment Analysis (ssGSEA) and Counting Relative Subsets of RNA Transcripts (CIBERSORT), we identified possible immunologic mechanisms, which led us to conclude that patients with high OSA risk tend to have elevated inflammation levels that can be brought down by CPAP treatment.
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http://dx.doi.org/10.3389/fgene.2022.927545DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326093PMC
July 2022

Polysomnographic features of idiopathic restless legs syndrome: a systematic review and meta-analysis of 13 sleep parameters and 23 leg movement parameters.

J Clin Sleep Med 2022 Jul 29. Epub 2022 Jul 29.

Sleep Medicine Center, Department of Respiratory and Critical Care Medicine, Mental Health Center, Translational Neuroscience Center, and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.

Study Objectives: This study aims to explore the polysomnographically measured sleep and leg movement (LM) differences between idiopathic restless legs syndrome (RLS) patients and healthy controls (HCs).

Methods: An electronic literature search was conducted in EMBASE, MEDLINE, All EBM databases, CINAHL, and PsycINFO. Only observational case-control studies were included in the meta-analysis. The differences in 13 sleep parameters and 23 LM parameters between RLS patients and HCs were explored.

Results: Thirty-eight studies were identified for systematic review, 31 of which were used for meta-analysis. Meta-analyses revealed significant reductions in total sleep time, sleep efficiency, Stage 2 (N2) and rapid eye movement (REM) sleep percentage, and increases in wake time after sleep onset, stage shifts per hour, Stage 1 (N1) percentage, REM latency, arousal index, and apnea-hypopnea index. Some LM parameters, such as periodic limb movement during sleep (PLMS) index, PLMS sequence duration, number of PLMS sequence, and periodicity index (PI), were higher in RLS patients compared with HCs. Further, our meta-analysis revealed a higher PLMS index during NREM sleep compared with that during REM sleep.

Conclusions: RLS patients manifest a lightening of sleep, increased sleep fragmentation and greater sleep related breathing disruption and limb movements during sleep relative to healthy normal subjects. The distributions of PLMS during a night's sleep may provide more information to clarify the specific characteristics of LM in RLS. PLMS in RLS are concentrated in NREM sleep. PI may be a more sensitive and specific marker of RLS than the PLMS index.
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http://dx.doi.org/10.5664/jcsm.10160DOI Listing
July 2022

The Mechanism of Chlorantraniliprole Resistance and Detoxification in Trichogramma chilonis (Hymenoptera: Trichogrammatidae).

J Insect Sci 2022 Jul;22(4)

College of Plant Protection, Shanxi Agricultural University, Taiyuan 030031, China.

Parasitic Trichogramma chilonis Ishii, an egg parasitoid of Grapholita molesta, is a critical agent for biological control of insect pests in crop plants. However, the efficiency of T. chilonis is influenced by its resistance to the common pesticide chlorantraniliprole. To elucidate the chlorantraniliprole detoxification mechanism, differentially expressed genes (DEGs) related to chlorantraniliprole resistance were studied at different developmental stages of the wasp. Individuals of T. chilonis were grouped and treated with chlorantraniliprole at different developmental stages. Untreated wasps were used as controls. Transcriptomic analysis identified the DEGs associated with chlorantraniliprole resistance and detoxification in T. chilonis. A total of 1,483 DEGs were associated with chlorantraniliprole resistance at all developmental stages. DEGs that correlated with chlorantraniliprole sensitivity of T. chilonis at different developmental stages were distinct and had various functions. The newly identified DEGs are involved in cytochrome P450- and glutathione metabolism-related pathways, which were predicted to contribute to chlorantraniliprole detoxification. Chlorantraniliprole detoxification by T. chilonis was associated with cytochrome P450- and glutathione-related pathways. Our findings may be useful for balancing chemical and biological control practices aimed to optimize agricultural production.
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http://dx.doi.org/10.1093/jisesa/ieac044DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334689PMC
July 2022

Modified constraint-induced movement therapy enhances cortical plasticity in a rat model of traumatic brain injury: a resting-state functional MRI study.

Neural Regen Res 2023 Feb;18(2):410-415

Center of Rehabilitation Medicine, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine; Institute of Rehabilitation Medicine, School of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine; Engineering Research Center of Traditional Chinese Medicine Intelligent Rehabilitation, Ministry of Education, Shanghai, China.

Modified constraint-induced movement therapy (mCIMT) has shown beneficial effects on motor function improvement after brain injury, but the exact mechanism remains unclear. In this study, amplitude of low frequency fluctuation (ALFF) metrics measured by resting-state functional magnetic resonance imaging was obtained to investigate the efficacy and mechanism of mCIMT in a control cortical impact (CCI) rat model simulating traumatic brain injury. At 3 days after control cortical impact model establishment, we found that the mean ALFF (mALFF) signals were decreased in the left motor cortex, somatosensory cortex, insula cortex and the right motor cortex, and were increased in the right corpus callosum. After 3 weeks of an 8-hour daily mCIMT treatment, the mALFF values were significantly increased in the bilateral hemispheres compared with those at 3 days postoperatively. The mALFF signal values of left corpus callosum, left somatosensory cortex, right medial prefrontal cortex, right motor cortex, left postero dorsal hippocampus, left motor cortex, right corpus callosum, and right somatosensory cortex were increased in the mCIMT group compared with the control cortical impact group. Finally, we identified brain regions with significantly decreased mALFF values at 3 days postoperatively. Pearson correlation coefficients with the right forelimb sliding score indicated that the improvement in motor function of the affected upper limb was associated with an increase in mALFF values in these brain regions. Our findings suggest that functional cortical plasticity changes after brain injury, and that mCIMT is an effective method to improve affected upper limb motor function by promoting bilateral hemispheric cortical remodeling. mALFF values correlate with behavioral changes and can potentially be used as biomarkers to assess dynamic cortical plasticity after traumatic brain injury.
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http://dx.doi.org/10.4103/1673-5374.344832DOI Listing
February 2023

Large-Scale Customized Production Scheduling of Multiagent-Based Medical 3D Printing.

Comput Intell Neurosci 2022 18;2022:6557137. Epub 2022 Jul 18.

Department of Neurosurgery, Third Affiliated Hospital, Naval Medical University, Shanghai 200438, China.

Three-dimensional (3D) printing, also known as additive manufacturing, has unique advantages over traditional manufacturing technologies; thus, it has attracted widespread attention in the medical field. Especially in the context of the frequent occurrence of major public health events, where the medical industry's demand for large-scale and customized production is increasing, traditional 3D printing production scheduling methods take a long time to handle large-scale customized medical 3D printing (M-3DP) production and have weak intelligent collaboration ability in the face of job-to-device matching under multimaterial printing. Given the problem caused by M-3DP large-scale customized production scheduling, an intelligent collaborative scheduling multiagent-based method is proposed in this study. First, a multiagent-based optimization model is established. On this basis, an improved genetic algorithm embedded with the product mix strategy and the intelligent matching mechanism is designed to optimize the completion time and load balance between devices. Finally, the effectiveness of the proposed method is evaluated using numerical simulation. The simulation results indicated that compared with the simple genetic algorithm, particle swarm optimization, and snake optimizer, the improved genetic algorithm could better reduce the M-3DP mass customization production scheduling time, optimize the load balance between devices, and promote the "intelligent manufacturing" process of M-3DP mass customization.
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http://dx.doi.org/10.1155/2022/6557137DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313918PMC
July 2022

Emerging mechanisms of pyroptosis and its therapeutic strategy in cancer.

Cell Death Discov 2022 Jul 27;8(1):338. Epub 2022 Jul 27.

Department of Oncology, NHC Key Laboratory of Cancer Proteomics & State Local Joint Engineering Laboratory for Anticancer Drugs, National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, 410008, Changsha, Hunan, China.

Pyroptosis, a type of inflammatory programmed cell death, is triggered by caspase cleavage of gasdermin family proteins. Based on accumulating evidence, pyroptosis is closely associated with tumour development, but the molecular mechanism underlying pyroptosis activation and the signalling pathways regulated by pyroptosis remain unclear. In this review, we first briefly introduce the definition, morphological characteristics, and activation pathways of pyroptosis and the effect of pyroptosis on anticancer immunity. Then we review recent progress concerning the complex role of pyroptosis in various tumours. Importantly, we summarise various FDA-approved chemotherapy drugs or natural compounds that exerted antitumor properties by inducing pyroptosis of cancer cells. Moreover, we also focus on the current application of nanotechnology-induced pyroptosis in tumour therapy. In addition, some unsolved problems and potential future research directions are also raised.
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http://dx.doi.org/10.1038/s41420-022-01101-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329358PMC
July 2022

Research on the Equity and Influencing Factors of Medical and Health Resources Allocation in the Context of COVID-19: A Case of Taiyuan, China.

Healthcare (Basel) 2022 Jul 16;10(7). Epub 2022 Jul 16.

School of Geographical Sciences, Shanxi Normal University, Taiyuan 030031, China.

COVID-19 has killed millions of people worldwide. As a result, medical and health resources continue to be strained, posing a great threat to people's safety and economic and social development. This paper built the index system of influencing factors of medical and health resources containing the economy, population and society, and then classified Taiyuan into three types of regions by cluster analysis. The Gini coefficient, Theil index and agglomeration degree were then used to analyze the spatial distribution of medical and health resources allocation, and its influencing factors were studied by grey relational analysis. It was found that the population allocation of medical and health resources in Taiyuan was better than area allocation. Population has the greatest influence on the allocation of medical and health resources, followed by society and the economy. The more developed the regional economy, the more diversified the main influencing factors, and the more adjustment and control choices of medical and health resources allocation. Suggestions for optimal allocation were put forward in order to fully utilize the limited medical and health resources, effectively respond to the epidemic needs, promote the sustainable development of resources, protect the health of residents, and improve social benefits.
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http://dx.doi.org/10.3390/healthcare10071319DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9324996PMC
July 2022

Renal phenotypes correlate with genotypes in unrelated individuals with tuberous sclerosis complex in China.

Orphanet J Rare Dis 2022 07 23;17(1):288. Epub 2022 Jul 23.

Department of Urology, Xiangya Hospital, Central South University, No. 87 Xiangya Road, Changsha City, 410008, Hunan Province, People's Republic of China.

Purpose: To explore the relationship between the genotype and renal phenotype in a Chinese cohort and guide clinical decision-making for treating tuberous sclerosis complex (TSC).

Materials And Methods: We reviewed 173 patients with definite TSC at three centers in China from September 2014 to September 2020. All the patients underwent TSC1 and TSC2 genetic testing as well as renal phenotypic evaluation. All analyses were performed using the SPSS software, version 19.0, with a cut-off P value of 0.05 considered statistically significant.

Results: We identified variants in 93% (161/173) cases, including 16% TSC1 and 77% TSC2 variants. Analysis of the relationship between the genotype and renal phenotype, revealed that those with TSC2 variants were more likely to develop severe renal AML (> 4) (P = 0.044). In terms of treatment, TSC2 variants were more likely to undergo nephrectomy/partial nephrectomy (P = 0.036) and receive mTOR medication such as everolimus (P < 0.001). However, there was no significant difference between the two groups in terms of their response to the everolimus treatment.

Conclusion: Patients with TSC2 variants exhibit more severe renal phenotypes, especially those associated with renal angiomyolipomas (AML), and they often require nephrectomy/partial nephrectomy or mTOR medication. Detection of the genotype is helpful in TSC management.
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http://dx.doi.org/10.1186/s13023-022-02443-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9308181PMC
July 2022

PD-1 inhibitors plus chemotherapy in EGFR/ALK-positive NSCLC patients with brain metastases and disease progression after EGFR/ALK-TKIs therapy.

J Cancer Res Clin Oncol 2022 Jul 20. Epub 2022 Jul 20.

Department of Medical Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China.

Background: Resistance to epidermal growth factor receptor (EGFR)/anaplastic lymphoma kinase (ALK)-tyrosine kinase inhibitors (TKIs) is a pervasive barrier in TKIs therapy for EGFR/ALK-positive non-small cell lung cancer (NSCLC) patients. Immune checkpoint inhibitor (ICI) monotherapy has exhibited an encouraging anti-tumor activity in high-selected EGFR/ALK-positive NSCLC patients with acquired resistance to TKI therapy. However, the effect of ICI plus chemotherapy therapy on those with brain metastases in this subset of patients is still unknown.

Methods: From April 2019 to August 2021, EGFR-mutated or ALK-rearranged NSCLC patients who progressed after previous EGFR/ALK-TKIs with brain metastases and received ICI plus chemotherapy ± bevacizumab at Cancer Hospital of the Chinese Academy of Medical Sciences (CAMS) were included. We retrospectively analyzed the efficacy, toxicity and progression site after ICI treatment.

Results: A total of 19 patients were included in the study, including 16 (84.4%) patients with EGFR mutations, 2 (10.5%) with ALK translocations and 1 (5.3%) with RET rearrangement. All of the patients progressed after previous TKI therapy and had brain metastatic lesions when received ICI combination therapy. The overall response rate (ORR) and disease control rate (DCR) were 15.8 and 57.9%, respectively. The median progression-free survival (PFS) and overall survival (OS) were 4.7 months (95% confidence interval CI 0.43-8.96) and 19.2 months (95% CI 15.08-23.29), respectively. The intracranial ORR was 10.5% and extracranial ORR was 15.8%, and the intracranial and extracranial DCR were 68.4 and 63.2%, respectively. The most common progression pattern was extracranial failure, and primary lesions enlargement rather than new sites metastases accounted for the vast majority of progressions. The most common grade 3-4 adverse event (AE) was leukopenia (31.6%), followed by neutropenia (26.3%), thrombocytopenia (10.5%) and rash (5.3%) successively. No grade 5 AE and discontinuation of ICI therapy for severe AEs were observed.

Conclusions: ICI combined with chemotherapy ± bevacizumab might be effective and safe for EGFR/ALK-positive NSCLC patients who progressed after previous TKI therapy, and synergistic anti-tumor activity for brain metastases was also observed.
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http://dx.doi.org/10.1007/s00432-022-04177-wDOI Listing
July 2022

Comparative efficacy analysis of ultrasound-guided quadratus lumborum block and lumbar plexus block in hip arthroscopy: a pilot prospective randomized controlled trial.

J Hip Preserv Surg 2022 Jul 29;9(2):119-125. Epub 2022 Mar 29.

Department of Anesthesiology, Beijing Jishuitan Hospital, Beijing 100000, China.

Controlled trials assessing quadratus lumborum block (QLB) for post-operative analgesia in hip surgery are scarce. This study aimed to compare ultrasound-guided QLB and lumbar plexus block (LPB) for clinical efficacy in hip arthroscopy. Patients undergoing hip arthroscopy in Beijing Jishuitan Hospital in January-June 2019 were randomized to the lumbar plexus (L) and quadratus lumborum (Q) groups ( = 25/group). After either ultrasound-guided block for 30 min, both groups were prepared for surgery after muscle strength measurement in the affected limbs. Opioid doses for patient-controlled analgesia (PCA), visual analog scale (VAS) scores in the resting and active states, upon leaving the post-anesthesia care unit (PACU), and at 2-48 h post-surgery were recorded, and post-operative complications were also recorded. Muscle strength in the affected limbs was significantly higher in the Q group compared with the L group (4.0 versus 2.0,  < 0.001). VAS scores were similar in both groups post-surgery ( > 0.05). One patient had epidural spread in the L group, with no other complications. Compared with ultrasound-guided LPB, ultrasound-guided QLB provides similar and good post-operative analgesia after hip arthroscopy, with less impact on muscle strength and fewer complications. These results should be confirmed in larger trials.
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http://dx.doi.org/10.1093/jhps/hnac020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9291388PMC
July 2022

The pH sensor and ion binding of NhaD Na /H antiporter from IT superfamily.

Mol Microbiol 2022 Jul 19. Epub 2022 Jul 19.

State Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University, Qingdao, P. R. China.

Sodium-proton (Na /H ) antiporters from the ion transporter (IT) superfamily play a vital role in controlling the pH and electrolyte homeostasis. However, very limited information regarding their structural functions is available to date. In this study, the structural model of the NhaD antiporter was proposed as a typical hairpin structure of IT proteins, with two symmetrically conserved scaffold domains that frame the core substrate-binding sites, and four motifs were identified. Furthermore, 25 conserved sites involving these domains were subjected to site-directed mutagenesis, and all mutations resulted in an impact on transport abilities. In particular, as candidates for Na -binding sites, D166 and D405 mutations at hairpin discontinuities were detrimental to transport activities and were found to induce pronounced conformational changes using fluorescence resonance energy transfer (FRET) assays. In addition, as observed in the NhaA structure, some charged residues, for example, E64, E65, R454, and R464, are predicted to be involved in the net charge switch of NhaD activation, by collectively form a "pH sensor" at the entrance of the cytoplasmic funnel. Mutations encompassing these residues were detrimental to the transport activity of NhaD or lost the capacity to respond to pH signals and trigger conformational changes for Na translocation.
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http://dx.doi.org/10.1111/mmi.14965DOI Listing
July 2022

Single-cell transcriptomic profiling to evaluate the effects of Di(2-ethylhexyl)phthalate exposure on early meiosis of female mouse germ cells.

Chemosphere 2022 Jul 13;307(Pt 1):135698. Epub 2022 Jul 13.

College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, 266109, China. Electronic address:

Di(2-ethylhexyl)phthalate (DEHP) has proven characteristics of an endocrine-disrupting compound (EDC), which can threaten the reproductive health of humans and other animals. In mammals, a series of chromosomal events occur during the meiotic stage of oocytes. External toxins may enter the body and cause infertility and other related diseases. Therefore, it is crucial to explore the influence of DEHP exposure on the molecular mechanism of germ cell meiosis. We used single-cell RNA sequencing (scRNA-seq) to analyse the ovaries of foetal mice at embryonic day 12.5 (E12.5) and E14.5 after maternal DEHP exposure. DEHP exposure further activated the pathways related to DNA repair in germ cells, increased the expression of genes related to DNA damage and changed the developmental trajectory of germ cells. DEHP exposure may affect the proliferation of pregranulosa (PG) cells. Moreover, DEHP exposure altered the signal transduction between PG cells and germ cells. We showed that DEHP affects meiosis by causing DNA damage in oocytes and disrupting the signal transduction between PG cells and germ cells. These results provide a strong theoretical basis for the prevention and treatment of DEHP-mediated female reproductive health problems.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135698DOI Listing
July 2022

Constructing ECM-like Structure on the Plasma Membrane via Peptide Assembly to Regulate the Cellular Response.

Langmuir 2022 Jul 15;38(29):8733-8747. Epub 2022 Jul 15.

Active Soft Matter Group, CAS Songshan Lake Materials Laboratory, Dongguan 523808, China.

This feature article introduces the design of self-assembling peptides that serve as the basic building blocks for the construction of extracellular matrix (ECM)-like structure in the vicinity of the plasma membrane. By covalently conjugating a bioactive motif, such as membrane protein binding ligand or enzymatic responsive building block, with a self-assembling motif, especially the aromatic peptide, a self-assembling peptide that retains bioactivity is obtained. Instructed by the target membrane protein or enzyme, the bioactive peptides self-assemble into ECM-like structure exerting various stimuli to regulate the cellular response via intracellular signaling, especially mechanotransduction. By briefly summarizing the properties and applications (e.g., wound healing, controlling cell motility and cell fate) of these peptides, we intend to illustrate the basic requirements and promises of the peptide assembly as a true bottom-up approach in the construction of artificial ECM.
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http://dx.doi.org/10.1021/acs.langmuir.2c00711DOI Listing
July 2022

SLC26A4 correlates with homologous recombination deficiency and patient prognosis in prostate cancer.

J Transl Med 2022 07 14;20(1):313. Epub 2022 Jul 14.

Department of Urology, Disorders of Prostate Cancer Multidisciplinary Team, Xiangya Hospital, Central South University, No. 87 Xiangya Road, Changsha, 410008, Hunan, People's Republic of China.

Background: Homologous recombination deficiency (HRD) is closely associated with patient prognosis and treatment options in prostate cancer (PCa). However, there is a lack of quantitative indicators related to HRD to predict the prognosis of PCa accurately.

Methods: We screened HRD-related genes based on the HRD scores and constructed an HRD cluster system to explore different clinicopathological, genomic, and immunogenomic patterns among the clusters. A risk signature, HRDscore, was established and evaluated by multivariate Cox regression analysis. We noticed that SLC26A4, a model gene, demonstrated unique potential to predict prognosis and HRD in PCa. Multi-omics analysis was conducted to explore its role in PCa, and the results were validated by qRT-PCR and immunohistochemistry.

Results: Three HRD clusters were identified with significant differences in patient prognosis, clinicopathological characteristics, biological pathways, immune infiltration characteristics, and regulation of immunomodulators. Further analyses revealed that the constructed HRDscore system was an independent prognostic factor of PCa patients with good stability. Finally, we identified a single gene, SLC26A4, which significantly correlated with prognosis in three independent cohorts. Importantly, SLC26A4 was confirmed to distinguish PCa (AUC for mRNA 0.845; AUC for immunohistochemistry score 0.769) and HRD (AUC for mRNA 0.911; AUC for immunohistochemistry score 0.689) at both RNA and protein levels in our cohort.

Conclusion: This study introduces HRDscore to quantify the HRD pattern of individual PCa patients. Meanwhile, SLC26A4 is a novel biomarker and can reasonably predict the prognosis and HRD in PCa.
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http://dx.doi.org/10.1186/s12967-022-03513-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9281181PMC
July 2022

Five-year incidence of primary glaucoma and related risk factors - The Handan eye study.

Acta Ophthalmol 2022 Jul 13. Epub 2022 Jul 13.

Beijing Tongren Eye Center, Beijing Key Laboratory of Ophthalmology and Visual Science, Beijing Tongren Hospital, Capital Medical University, Beijing, China.

Purpose: To determine the 5-year incidence of primary glaucoma and its associated risk factors in rural northern China.

Methods: Population-based cohort study. A total of 5184 participants aged 30 years and older, without glaucoma at baseline, were subjected to comprehensive standardized interviews and ophthalmic and systemic examinations at baseline and after a 5-year interval in the Handan Eye Study. Incident glaucoma was diagnosed by a consensus panel of five senior glaucoma specialists. Univariate and multivariable logistic regression analyses were performed to identify the baseline risk factors that could predict the incidence of glaucoma.

Results: During the 5-year follow-up, incident primary glaucoma developed in 82 subjects (1.6%; 95% confidence interval [CI], 1.2%-1.9%). The age- and gender-standardized incidence of glaucoma among subjects ≥40 years old was 2.1% (0.4% annually), calculated according to the 2010 Chinese census. A higher age (odds ratio [OR], 1.06; 95% CI, 1.04-1.09; p < 0.001), higher intraocular pressure (IOP) (OR, 1.11; 95% CI, 1.02-1.20; p = 0.017), and vertical cup disc ratio (VCDR) ≥ 0.60 (OR, 5.30; 95% CI, 3.22-8.73; p < 0.001) were found to be associated with an increased risk of incident glaucoma. For each year, older age and each mmHg higher IOP, the risks of primary glaucoma increased by 1.2% and 2.0% per year, respectively.

Conclusion: We reported the 5-year incidence of primary glaucoma in a rural Chinese population and found that older age, higher IOP, and VCDR ≥ 0.60 at baseline could help in identifying those at highest risk of disease development.
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http://dx.doi.org/10.1111/aos.15216DOI Listing
July 2022

A Dof-CLE circuit controls phloem organization.

Nat Plants 2022 07 11;8(7):817-827. Epub 2022 Jul 11.

Graduate School of Science, Osaka University, Toyonaka, Osaka, Japan.

The phloem consists of sieve elements (SEs) and companion cells (CCs). Here we show that Dof-class transcription factors preferentially expressed in the phloem (phloem-Dofs) are not only necessary and sufficient for SE and CC differentiation, but also induce negative regulators of phloem development, CLAVATA3/EMBRYO SURROUNDING REGION-RELATED25 (CLE25), CLE26 and CLE45 secretory peptides. CLEs were perceived by BARELY ANY MERISTEM (BAM)-class receptors and CLAVATA3 INSENSITIVE RECEPTOR KINASE (CIK) co-receptors, and post-transcriptionally decreased phloem-Dof proteins and repressed SE and CC formation. Multiple mutations in CLE-, BAM- or CIK-class genes caused ectopic formation of SEs and CCs, producing an SE/CC cluster at each phloem region. We propose that while phloem-Dofs induce phloem cell formation, they inhibit excess phloem cell formation by inducing CLEs. Normal-positioned SE and CC precursor cells appear to overcome the effect of CLEs by reinforcing the production of phloem-Dofs through a positive feedback transcriptional regulation.
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http://dx.doi.org/10.1038/s41477-022-01176-0DOI Listing
July 2022

Peroxisome Proliferator-Activated Receptor Gene Knockout Promotes Podocyte Injury in Diabetic Mice.

Biomed Res Int 2022 30;2022:9018379. Epub 2022 Jun 30.

Guizhou Provincial Key Laboratory of Pathogenesis and Drug Research on Common Chronic Diseases, Guizhou Medical University, Guiyang, 550025 Guizhou, China.

Objective: To investigate the effects of peroxisome proliferator-activated receptor (PPAR) expression on renal podocyte in diabetic mice by conditionally knockout mouse PPAR gene.

Methods: Wild-type C57BL mice and PPAR gene knockout mice were used as research objects to establish the diabetic mouse model, which was divided into normal control group (NC group), normal glucose PPAR gene knockout group (NK group), diabetic wild-type group (DM group), and diabetic PPAR gene knockout group (DK group), with 8 mice in each group. After 16 weeks, the mice were sacrificed for renal tissue collection. Morphological changes of renal tissue were observed by HE and Masson staining, and ultrastructure of renal tissue was observed by transmission electron microscope. Protein expressions of PPAR, podocin, nephrin, collagen IV, and fibronectin (FN) in renal tissues were detected by immunohistochemistry and Western blot, and mRNA changes of PPAR, podocin, and nephrin in renal tissues were detected by qRT-PCR.

Results: Compared with the NC group, the protein and mRNA expressions of PPAR, podocin, and nephrin decreased in the kidney tissue of mice in the DM group, while the protein expressions of collagen IV and FN increased. The expression of various proteins in kidney tissues of the DK group was consistent with that of the DM group, and the difference was more obvious. The expression of PPAR protein and mRNA decreased in the NK group, while the expression of podocin, nephrin protein and mRNA, collagen IV, and FN protein showed no significant difference.

Conclusion: In diabetic renal tissue, the loss of PPAR can aggravate podocellular damage and thus promote the occurrence of diabetic renal fibrosis. Increasing the expression of PPAR may effectively relieve renal podocyte impairment in diabetic patients, which can be used for the treatment of diabetic nephropathy.
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http://dx.doi.org/10.1155/2022/9018379DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9262558PMC
July 2022

Esterase-responsive and size-optimized prodrug nanoparticles for effective intracranial drug delivery and glioblastoma treatment.

Nanomedicine 2022 Jul 8;44:102581. Epub 2022 Jul 8.

Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, China; Central Laboratory, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, China. Electronic address:

Glioblastoma multiforme (GBM) is the intracranial malignancy with the highest rates of morbidity and mortality. Chemotherapy is often ineffective against GBM due to the presence of the blood-brain barrier (BBB); however, the application of nanotechnology is expected to overcome this limitation. Poly(lactic-co-glycolic acid) (PLGA) is a degradable and nontoxic functional polymer with good biocompatibility that is widely used in the pharmaceutical industry. Previous studies have shown that the ability of PLGA nanoparticles (NPs) to penetrate the BBB is largely determined by their size; however, determination of the optimal PLGA NP size requires further research. Here, we report a tandutinib-based prodrug (proTan), which responds to the GBM microenvironment, that was combined with NPs to overcome the BBB. AMD3100-PLGA NPs loaded with proTan inhibited tumor growth and effectively prolonged the survival of tumor-bearing mice.
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http://dx.doi.org/10.1016/j.nano.2022.102581DOI Listing
July 2022

Tellurium Nanotubes and Chemical Analogues from Preparation to Applications: A Minor Review.

Nanomaterials (Basel) 2022 Jun 22;12(13). Epub 2022 Jun 22.

School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China.

Tellurium (Te), the most metallic semiconductor, has been widely explored in recent decades owing to its fantastic properties such as a tunable bandgap, high carrier mobility, high thermal conductivity, and in-plane anisotropy. Many references have witnessed the rapid development of synthesizing diverse Te geometries with controllable shapes, sizes, and structures in different strategies. In all types of Te nanostructures, Te with one-dimensional (1D) hollow internal structures, especially nanotubes (NTs), have attracted extensive attention and been utilized in various fields of applications. Motivated by the structure-determined nature of Te NTs, we prepared a minor review about the emerging synthesis and nanostructure control of Te NTs, and the recent progress of research into Te NTs was summarized. Finally, we highlighted the challenges and further development for future applications of Te NTs.
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http://dx.doi.org/10.3390/nano12132151DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268052PMC
June 2022

Pericycle cell division competence underlies various developmental programs.

Plant Biotechnol (Tokyo) 2022 Mar;39(1):29-36

Department of Biology, Graduate School of Science, Osaka University, Osaka 560-0043, Japan.

Pericycle cells possess proliferative activity long after leaving the root apical meristem. Depending on the developmental stage and external stimuli, pericycle cell division leads to the production of lateral roots, vascular cambium and periderm, and callus. Therefore, pericycle cell division competence underlies root branching and secondary growth, as well as plant regeneration capacity. In this review, we first briefly present an overview of the molecular pathways of the four developmental programs originated, exclusively or partly, from pericycle cells. Then, we provide a review of up-to-date knowledge in the mechanisms determining pericycle cells' competence to undergo cell division. Furthermore, we discuss directions of future research to further our understanding of the pericycle's characteristics and functions.
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http://dx.doi.org/10.5511/plantbiotechnology.21.1202aDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200087PMC
March 2022

Applications and Achievements of Single-Cell Sequencing in Gastrointestinal Cancer.

Front Oncol 2022 16;12:905571. Epub 2022 Jun 16.

The Pathophysiology Department, School of Basic Medical Sciences, College of Medicine, Zhengzhou University, Zhengzhou, China.

Gastrointestinal cancer represents a public health concern that seriously endangers human health. The emerging single-cell sequencing (SCS) technologies are different from the large-scale sequencing technologies which provide inaccurate data. SCS is a powerful tool for deciphering the single-cell resolutions of cellular and molecular landscapes, revealing the features of single-cell genomes, transcriptomes, and epigenomes. Recently, SCS has been applied in the field of gastrointestinal cancer research for clarifying the origin and heterogeneity of gastrointestinal cancer, acquiring micro-environmental information, and improving diagnostic and treatment methods. This review outlines the applications of SCS in gastrointestinal cancer research and summarizes the most recent advances in the field.
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http://dx.doi.org/10.3389/fonc.2022.905571DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9245065PMC
June 2022
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