Publications by authors named "Ye Wang"

1,234 Publications

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Phosphorylated α-synuclein aggregated in Schwann cells exacerbates peripheral neuroinflammation and nerve dysfunction in Parkinson's disease through TLR2/NF-κB pathway.

Cell Death Discov 2021 Oct 12;7(1):289. Epub 2021 Oct 12.

Department of Neurology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

To investigate the mechanism of peripheral neuropathy in Parkinson's disease (PD), we prepared a PD mice model by long-term exposure of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) to mimic PD pathology in humans and the sciatic nerves were taken for further research. It turned out that phosphorylated α-synuclein (p-α-syn) was significantly deposited in Schwann cells (SCs) of sciatic nerves possibly contributing to degenerated myelin SCs and atrophied axons in MPTP group. Further analysis confirmed that toll-like receptors (TLRs) were implicated with PD peripheral neuropathy, in which TLR2 exhibits the predominant expression. Increased expression of inflammatory factors about TLR2/nuclear factor kappa-B (NF-κB) pathway was noted in MPTP group compared to saline group, with proteins on other pathways showing no changes. Moreover, MPTP-challenged mice exhibited worse motor ability and damaged nerve conduction, implicating that p-α-syn neurotoxicity might be relevant to impairments of motor and sensory nerves. After the treatment of CU-CPT22, a TLR2 antagonist, p-α-syn accumulation, motor and sensory function were ameliorated in CU-CPT22 combined with MPTP group. Thus, we demonstrated that pathological p-α-syn might combine TLR2 to affect SCs activation, inflammatory response as well as motor and sensory function through TLR2/nuclear factor kappa-B (NF-κB) signaling pathway. This study firstly demonstrates a novel mechanism of p-α-syn accumulated in SCs of peripheral nerves, which extends our understanding on SCs-mediated peripheral neuroinflammation related to TLR2/NF-κB signaling pathway and sheds light on potential new therapeutic avenues for PD.
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http://dx.doi.org/10.1038/s41420-021-00676-wDOI Listing
October 2021

Solid-Contact Ion-Selective Electrodes for Histamine Determination.

Sensors (Basel) 2021 Oct 7;21(19). Epub 2021 Oct 7.

State Key Laboratory of Industrial Control Technology, College of Control Science and Engineering, Zhejiang University, Hangzhou 310027, China.

Solid-contact ion-selective electrodes for histamine (HA) determination were fabricated and studied. Gold wire (0.5 mm diameter) was coated with poly(3,4-ethlenedioxythiophene) doped with poly(styrenesulfonate) (PEDOT:PSS) as a solid conductive layer. The polyvinyl chloride matrix embedded with 5,10,15,20-tetraphenyl(porphyrinato)iron(iii) chloride as an ionophore, 2-nitrophenyloctyl ether as a plasticizer and potassium tetrakis(p-chlorophenyl) borate as an ion exchanger was used to cover the PEDOT:PSS layer as a selective membrane. The characteristics of the HA electrodes were also investigated. The detection limit of 8.58 × 10 M, the fast response time of less than 5 s, the good reproducibility, the long-term stability and the selectivity in the presence of common interferences in biological fluids were satisfactory. The electrode also performed stably in the pH range of 7-8 and the temperature range of 35-41 °C. Additionally, the recovery rate of 99.7% in artificial cerebrospinal fluid showed the potential for the electrode to be used in biological applications.
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http://dx.doi.org/10.3390/s21196658DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8512055PMC
October 2021

Towards Dendrite-Free Potassium-Metal Batteries: Rational Design of a Multifunctional 3D Polyvinyl Alcohol-Borax Layer.

Angew Chem Int Ed Engl 2021 Sep 14. Epub 2021 Sep 14.

International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education, Institute of Microscale Optoelectronics, Shenzhen University, 3688 Nanhai Avenue, Nanshan District, Shenzhen, 518060, China.

K metal is the optimal anode for K-ion batteries because of its high capacity and low operating potential, but it suffers from fast capacity fading and safety issues due to an unstable solid electrolyte interphase (SEI) and continuous K-dendrite growth. Herein, to obtain promising potassium-metal batteries, a 3D polyvinyl-alcohol (PVA)-borax layer is designed, which enables a dendrite-free K-plating/stripping process. The protective layer possesses good wettability, high K-ion diffusivity, and good structural stability, which enables a "uniform and underneath plating" behavior, therefore exhibiting a stable electrochemical performance. As a result, Cu current collector with PVA-borax ([email protected]) exhibits a stable cycling lifetime for 700 h at 0.5 mA cm and 500 h at 1 mA cm at 10 % depth of discharge (DOD) without dendrite formation. Even at a high utilization of 25 % DOD and 50 % DOD, the [email protected] shows a stable cycle for 180 h and 100 h, respectively.
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http://dx.doi.org/10.1002/anie.202111753DOI Listing
September 2021

IL (Interleukin)-17A Acts in the Brain to Drive Neuroinflammation, Sympathetic Activation, and Hypertension.

Hypertension 2021 Nov 11;78(5):1450-1462. Epub 2021 Oct 11.

Department of Internal Medicine (Y.C., Y.Y., S.-G.W.), University of Iowa Carver College of Medicine.

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http://dx.doi.org/10.1161/HYPERTENSIONAHA.121.18219DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8516065PMC
November 2021

Electrocatalytic reduction of CO and CO to multi-carbon compounds over Cu-based catalysts.

Chem Soc Rev 2021 Oct 5. Epub 2021 Oct 5.

State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials, National Engineering Laboratory for Green Chemical Productions of Alcohols, Ethers and Esters, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM), College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.

The electrocatalytic reduction of CO with HO to multi-carbon (C) compounds, in particular, C olefins and oxygenates, which have versatile applications in the chemical and energy industries, holds great potential to mitigate the depletion of fossil resources and abate carbon emissions. There are two major routes for the electrocatalytic CO reduction to C compounds, , the direct route and the indirect route CO. The electrocatalytic CO reduction to CO has been commercialised with solid oxide electrolysers, making the indirect route CO to C compounds also a promising alternative. This tutorial review focuses on the similarities and differences in the electrocatalytic CO and CO reduction reactions (CORR and CORR) into C compounds, including CH, CHOH, CHCOO and -CHOH, over Cu-based catalysts. First, we introduce the fundamental aspects of the two electrocatalytic reactions, including the cathode and anode reactions, electrocatalytic reactors and crucial performance parameters. Next, the reaction mechanisms, in particular, the C-C coupling mechanism, are discussed. Then, efficient catalysts and systems for these two reactions are critically reviewed. We analyse the key factors that determine the selectivity, activity and stability for the electrocatalytic CORR and CORR. Finally, the opportunities, challenges and future trends in the electrocatalytic CORR and CORR are proposed. These insights will offer guidance for the design of industrial-relevant catalysts and systems for the synthesis of C olefins and oxygenates.
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http://dx.doi.org/10.1039/d1cs00535aDOI Listing
October 2021

Electron penetration triggering interface activity of Pt-graphene for CO oxidation at room temperature.

Nat Commun 2021 Oct 4;12(1):5814. Epub 2021 Oct 4.

State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials (iChEM), College of Chemistry and Chemical Engineering, Xiamen University, 361005, Xiamen, China.

Achieving CO oxidation at room temperature is significant for gas purification but still challenging nowadays. Pt promoted by 3d transition metals (TMs) is a promising candidate for this reaction, but TMs are prone to be deeply oxidized in an oxygen-rich atmosphere, leading to low activity. Herein we report a unique structure design of graphene-isolated Pt from CoNi nanoparticles (PtǀCoNi) for efficiently catalytic CO oxidation in an oxygen-rich atmosphere. CoNi alloy is protected by ultrathin graphene shell from oxidation and therefore modulates the electronic property of Pt-graphene interface via electron penetration effect. This catalyst can achieve near 100% CO conversion at room temperature, while there are limited conversions over Pt/C and Pt/CoNiO catalysts. Experiments and theoretical calculations indicate that CO will saturate Pt sites, but O can adsorb at the Pt-graphene interface without competing with CO, which facilitate the O activation and the subsequent surface reaction. This graphene-isolated system is distinct from the classical metal-metal oxide interface for catalysis, and it provides a new thought for the design of heterogeneous catalysts.
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http://dx.doi.org/10.1038/s41467-021-26089-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8490350PMC
October 2021

Construction of Low-Cost Z-Scheme Heterostructure Cu O/PCN for Highly Selective CO Photoreduction to Methanol with Water Oxidation.

Small 2021 Oct 4:e2103558. Epub 2021 Oct 4.

Institute for New Energy Materials and Low Carbon Technologies, School of Materials Science and Engineering, School of Chemistry and Chemical Engineering, Tianjin University of Technology, Tianjin, 300384, China.

Solar-driven CO reaction with water oxidation into alcohols represents a promising approach to achieve real artificial photosynthesis. However, rapid recombination of photogenerated carriers seriously restricts the development of artificial photosynthesis. Herein, a facile method is explored to construct low-cost Z-Scheme heterostructure Cu O/polymeric carbon nitride (PCN) by in situ growth of Cu O hollow nanocrystal on PCN. The protective PCN layer and Z-schematic charge flow can make robust Cu O/PCN photocatalysts, and the spatial separation of electrons and holes with high redox potentials of E (-1.15 eV) and E (1.65 eV) versus NHE can efficiently drive CO photoreduction to methanol in pure water, which is further confirmed by DFT calculation. The Z-scheme heterostructure Cu O/PCN exhibits a high methanol yield of 276 µmol g in 8 h with ca. 100% selectivity, much superior to that of isolated Cu O and PCN, and all the reported Cu O-based heterostructures. This work provides a unique strategy to efficiently and selectively promote the conversion of CO and H O into high-value chemicals by constructing a low-cost Z-scheme heterostructure.
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http://dx.doi.org/10.1002/smll.202103558DOI Listing
October 2021

Robotic-assisted radical cystectomy is associated with lower perioperative mortality in octogenarians.

Urol Oncol 2021 Sep 30. Epub 2021 Sep 30.

Division of Urologic Surgery, Brigham and Women's Hospital, Boston, MA.

Objectives: To compare perioperative outcomes between robotic and open radical cystectomy in octogenarians using real-world data SUBJECTS AND METHODS: Using the Premier Healthcare Database, we found 13,127 patients who underwent robotic-assisted radical cystectomy (RARC) between 2008 and 2017, of which 15.1% were ≥80 years old. Perioperative mortality was the primary outcome of interest. Secondary outcomes include complications, hospital length of stay, readmission rates, and disposition after discharge. Multivariable regression analysis was used to adjust for patient and hospital characteristics.

Results: In octogenarians, mortality at the index admission was 2.2% in those who underwent RARC, compared to 4.6% in those who underwent open surgery (P = 0.027). On multivariable analysis, robotic surgery was associated with lower in-hospital mortality in octogenarians (OR 0.46, 95% CI 0.22-0.99, P = 0.047) even after controlling for patient, and hospital characteristics.

Conclusion: RARC is safe and feasible in octogenarians. Elderly patients may derive more benefit from minimally invasive radical cystectomy compared to a younger cohort.
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http://dx.doi.org/10.1016/j.urolonc.2021.08.027DOI Listing
September 2021

Clonidine as an Additive to Local Anesthetics in Caudal Block for Postoperative Analgesia in Pediatric Surgery: A Systematic Review and Meta-Analysis.

Front Med (Lausanne) 2021 14;8:723191. Epub 2021 Sep 14.

Anesthesia and Operation Centre, First Medical Centre of Chinese PLA General Hospital, Beijing, China.

Clonidine is an anesthetic with favorable efficacy and safety profiles for caudal epidural block, but comparisons with other adjuvants need to be confirmed in pediatric patients. To investigate the effects of clonidine as an adjuvant in caudal epidural block to improve the intraoperative and postoperative analgesia in pediatric surgery. PubMed, Embase, and the Cochrane Library were searched for available papers published up to February 2021. The outcomes were pain score, duration of analgesia, complications, and number of analgesic requirements. The meta-analysis was performed using random-effects models. Fifteen randomized controlled trials (RCTs) were included. There were no differences between clonidine and the control drug regarding the duration of analgesia (SMD = -0.71, 95%CI: -1.64, 0.23; = 95.5%, P < 0.001), pain score (SMD = 0.35, 95%CI: -0.28, 0.98; = 80.8%, P < 0.001), and requirement for additional analgesia (OR = 8.77, 95%CI: 0.70, 110.58, = 81.9%, P = 0.004), but using clonidine resulted in fewer complications than the control drugs (OR = 0.33, 95%CI: 0.20, 0.54, = 21.8%, P = 0.217). The sensitivity analysis showed that the results were robust. A publication bias was observed. Clonidine has the same efficacy as the other adjuvants for caudal epidural block for pediatric surgery but fewer complications. These results support clonidine as an adjuvant to local anesthetic, but additional studies should be conducted.
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http://dx.doi.org/10.3389/fmed.2021.723191DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8476839PMC
September 2021

Monolithic integration of nanorod arrays on microfluidic chips for fast and sensitive one-step immunoassays.

Microsyst Nanoeng 2021 17;7:65. Epub 2021 Aug 17.

College of Information Science and Electronic Engineering, Zhejiang University, 310027 Hangzhou, People's Republic of China.

Here, we present integrated nanorod arrays on microfluidic chips for fast and sensitive flow-through immunoassays of physiologically relevant macromolecules. Dense arrays of Au nanorods are easily fabricated through one-step oblique angle deposition, which eliminates the requirement of advanced lithography methods. We report the utility of this plasmonic structure to improve the detection limit of the cardiac troponin I (cTnI) assay by over 6 × 10-fold, reaching down to 33.9 fg mL (~1.4 fM), compared with an identical assay on glass substrates. Through monolithic integration with microfluidic elements, the device enables a flow-through assay for quantitative detection of cTnI in the serum with a detection sensitivity of 6.9 pg mL (~0.3 pM) in <6 min, which was 4000 times lower than conventional glass devices. This ultrasensitive detection arises from the large surface area for antibody conjugation and metal-enhanced fluorescent signals through plasmonic nanostructures. Moreover, due to the parallel arrangement of flow paths, simultaneous detection of multiple cancer biomarkers, including prostate-specific antigen and carcinoembryonic antigen, has been fulfilled with increased signal-to-background ratios. Given the high performance of this assay, together with its simple fabrication process that is compatible with standard mass manufacturing techniques, we expect that the prepared integrated nanorod device can bring on-site point-of-care diagnosis closer to reality.
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http://dx.doi.org/10.1038/s41378-021-00291-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433357PMC
August 2021

Predictive value of serum HCG concentrations for outcomes of vitrified-warmed blastocyst transfers in women of different ages.

Reprod Biomed Online 2021 Jul 21. Epub 2021 Jul 21.

Department of Reproductive Medicine, Xiamen Maternity and Child Health Hospital, Xiamen, China; Xiamen Key Laboratory of Reproduction and Genetics, Xiamen, China.

Research Question: Can serum human chorionic gonadotrophin (HCG) concentrations on day 10 after single-blastocyst transfer (SBT) in cryopreserved transfer cycles help to predict the cycle outcome in patients of different maternal ages?

Design: The study included 772 vitrified-warmed SBT cycles. The initial maternal serum HCG concentrations measured on day 10 after blastocyst transfer were evaluated using receiver operating characteristic (ROC) curves to predict clinical pregnancy and live birth. Threshold values for predicting a clinical pregnancy were established in three different age groups: group A (21-29 years old, n = 360), group B (30-34 years old, n = 290) and group C (35-47 years old, n = 122).

Results: The areas under the ROC curves for clinical pregnancy and live birth were 0.986 and 0.922, and the corresponding cut-off values were 113.28 and 146.37 mIU/ml, respectively. The optimal threshold values for clinical pregnancy as indicated by Youden index values for the three age groups were 145.15, 126.25 and 94.44 mIU/ml, respectively.

Conclusions: The study demonstrates that determination of initial serum β-HCG concentrations on day 10 after SBT in cryopreserved transfer cycles can help to predict cycle outcome in women of different ages. The optimal threshold value for clinical pregnancy for patients over 35 years of age was lower than that for the younger age groups.
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http://dx.doi.org/10.1016/j.rbmo.2021.07.004DOI Listing
July 2021

DNA-guided photoactivatable probe-based chemical proteomics reveals the reader protein of mRNA methylation.

iScience 2021 Sep 28;24(9):103046. Epub 2021 Aug 28.

The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, Key Laboratory of Breast Cancer Prevention and Therapy (Ministry of Education), Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Tianjin Medical University Cancer Institute and Hospital, Tianjin Medical University General Hospital, Tianjin Medical University, Tianjin 300070, China.

Chemical modification on mRNA can recruit specific binding proteins (readers/partners) to determine post-transcriptional gene regulation. However, the identification of the reader is extremely limited owing to the rather weak and highly dynamic non-covalent interactions between mRNA modification and reader, and therefore the sensitive and robust approaches are desirable. Here, we report a DNA-guided photoactivatable-based chemical proteomic approach for profiling the readers of mRNA methylation. By use of N-methyladenosine (mA), we illustrated that this method can be successfully utilized for labelling and enriching the readers of mRNA modification, as well as for the discovery of new partners. Thus we applied this strategy to a new modification 2'-O-methyladenosine. As a result, DDX1 was identified and verified as a potential binding protein. Our study therefore provides a powerful chemical proteomics tool for identifying the binding factors of mRNA modification and reveals the underlying function of mRNA modification.
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http://dx.doi.org/10.1016/j.isci.2021.103046DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8441146PMC
September 2021

α-Actinin1 promotes tumorigenesis and epithelial-mesenchymal transition of gastric cancer via the AKT/GSK3β/β-Catenin pathway.

Bioengineered 2021 12;12(1):5688-5704

Department of Gastrointestinal Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.

α-Actinin1 (ACTN1), an actin cross-linking protein, is implicated in cytokinesis, cell adhesion, and cell migration. In addition, it is involved in the tumorigenesis and development of certain cancers, such as breast cancer. We explored the function of ACTN1 in gastric cancer (GC), which has largely remained unclear. High-throughput sequencing and public microarray datasets from the Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) revealed the upregulation of ACTN1 in gastric cancer with a poor prognosis. These results were further verified by western blotting (WB), Real-Time Quantitative polymerase chain reaction (RT-qPCR), and immunohistochemistry. We constructed loss and gain of function gastric cancer cells, which revealed the effect of ACTN1 over-expression on promoting GC cell proliferation, invasion, migration, and inhibited apoptosis. Mechanistic studies revealed that ACTN1 regulates the epithelial-mesenchymal transition (EMT) and tumorigenesis of gastric cancer via the AKT/GSK3β/β-catenin pathway, confirmed by the inhibitor of AKT MK2206. Altogether, these results demonstrated that ACTN1 could be a promising candidate for gastric cancer treatment.
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http://dx.doi.org/10.1080/21655979.2021.1967713DOI Listing
December 2021

Zero-Dimensional Organic-Inorganic Hybrid Copper-Based Halides with Highly Efficient Orange-Red Emission.

Small 2021 Sep 21:e2103831. Epub 2021 Sep 21.

International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education, Institute of Microscale Optoelectronics, Shenzhen University, Shenzhen, 518060, China.

Lead-free metal halides are promising luminescent materials with low non-toxicity, tunable emission, and high luminescence quantum yield (PLQY). The development of red light-emitting materials with high PLQY to meet the color coordinate requirements for light-emitting diode (LED) lighting and wide color gamut displays remains a major scientific challenge. Herein, novel lead-free copper-based halide materials are reported. PEA Cu I is an efficient orange-red broad emission with a highest PLQY of 73.6%. All three materials, namely, those of ((R)-(+)-α-PEA) Cu I , ((S)-(-)-α-PEA) Cu I , and (β-PEA) Cu I with zero-dimensional structures have large exciton binding energies and Huang-Rhys factors, thus causing efficient self-trapped exciton emissions. A prototypical orange-red LED device is fabricated successfully that demonstrates the potential of copper-based halide materials in lighting and display applications.
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http://dx.doi.org/10.1002/smll.202103831DOI Listing
September 2021

Shock in China 2018 (SIC-study): a cross-sectional survey.

Ann Transl Med 2021 Aug;9(15):1219

Department of Cardiology, Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing, China.

Background: Shock is a critical illness that seriously threatens the lives of patients. This study explains the epidemiology of shock, mortality of shock, and identify factors that related to hospital death.

Methods: This is a multi-centre cross-sectional survey, which included 1,064 tertiary hospitals in 31 provinces, municipalities, and autonomous regions across China mainland. Totally 289,428 patients who diagnosed with shock based on the ICD-10 abstracted from the Hospital Quality Monitoring System (HQMS) in 2018, a national database administrated by National Health Commission of the PRC.

Results: Patients diagnosed with shock were screened and classified according to the type of shock. Regression analysis was used to identify factors that related to death. A total of 79,668,156 medical records were included in HQMS in 2018, from which a total of 289,428 records with shock were identified. Hypovolemic shock occurred in 128,436 cases (44.38%), septic shock occurred in 121,543 cases (41.99%), cardiogenic shock occurred in 44,597 cases (15.41), and obstructive shock occurred in 3,168 cases (1.09%). Of these, 8,147 cases (2.81%) had mixed shock, which means had two or more types of shock. For all the shock cases, the top three frequent concomitant diseases recorded were circulatory system diseases (55.22%), digestive system diseases (53.64%), and respiratory system diseases (53.31%). Of the four types of shock, cases with cardiogenic shock had the highest in-hospital mortality (31.6%), followed by those with obstructive shock (25.2%), septic shock (22.9%), and hypovolemic shock (15.5%). Interestingly, the combination of shock and malignant tumors is one of the major factors that related to hospital deaths.

Conclusions: Shock is a serious disease with a high fatality rate and huge clinical costs. According to this epidemiological survey of shock in China 2018, we should clarify the factors related to the hospital death in shock cases.
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http://dx.doi.org/10.21037/atm-21-310DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421935PMC
August 2021

Association between Serum Uric Acid and Hypertension in Han and Yugur of Gansu Province: The China National Health Survey.

Kidney Blood Press Res 2021 Sep 10:1-11. Epub 2021 Sep 10.

Department of Epidemiology and Statistics, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing, China.

Introduction: Serum uric acid (SUA) has been found correlated with an increased risk of hypertension, but evidence is sparse regarding the association in Gansu Province, especially in Yugur people. This study aimed to explore the nonlinear relationship between SUA levels and hypertension in Han and Yugur people in China.

Methods: The cross-sectional study samples (n = 5,327) were from the China National Health Survey (CNHS) in Gansu Province. Participants were selected using a multistage stratified cluster sampling method. SUA was measured by enzymatic methods. The restricted cubic spline regression was performed to evaluate the shape of the association.

Results: The overall prevalence of hypertension and hyperuricemia was 28.4% and 17.0%, respectively, in this study. Comparing the highest (>416.4 μmol/L) to the lowest (<254.1 μmol/L) SUA level groups, the multivariable adjusted differences and 95% confidence intervals (CIs) in blood pressure (BP) were 6.15 (4.22, 8.08) mm Hg and 4.87 (3.51, 6.23) mm Hg for SBP and DBP in Han, and 2.22 (-0.73, 5.18) mm Hg and 2.56 (0.38, 4.75) mm Hg for SBP and DBP in Yugur people, respectively. The corresponding odds ratios (95% CIs) for hypertension were 3.16 (2.26, 4.43) and 2.37 (1.46, 3.89) in Han and Yugur people, respectively. The restricted cubic spline regression models illustrated that both BP level and the risk of hypertension increased with elevated SUA levels in Han and Yugur people.

Conclusions: SUA was significantly and independently associated with an increased risk of hypertension in Han and Yugur people. Prospective studies are needed to confirm these findings.
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http://dx.doi.org/10.1159/000518768DOI Listing
September 2021

Analysis of the role of METTL5 as a hub gene in lung adenocarcinoma based on a weighted gene co-expression network.

Math Biosci Eng 2021 08;18(5):6608-6619

Clinical Laboratory, Qingdao Central Hospital, The Second Affiliated Hospital of Medical College of Qingdao University, Qingdao, Shandong Province 266042, China.

Lung adenocarcinoma (LUAD) is a frequently diagnosed malignant tumor that is highly invasive and lethal. The prognosis of patients with LUAD still needs to be improved, as conventional treatment is remarkably well tolerated. In this study, the expression profile of LUAD in the TCGA database was used for differential expression analysis, and differential expression genes were determined to construct a weighted gene co-expression network analysis (WGCNA) for dividing and finding the gene modules with the highest correlation with tumor stage. Here, METTL5, DDX23, GPSM2, CEP95, WDCP, and METL17 were identified as hub genes. According to the relation degree, METTL5 was determined as the candidate gene in this study. Difference analysis and receiver operating characteristic (ROC) curve were applied to identify the predictive performance of METTL5 in LUAD, and Kaplan-Meier (KM) analysis showed that the prognosis of LUAD patients with high METTL5 expression was poor. Further GSEA analysis showed that high-expressed METTL5 was related to epithelial-mesenchymal transition and other pathways. Therefore, METTL5 may be involved in the occurrence and malignant progression of LUAD. The current findings provide an effective molecular target for early diagnosis of LUAD, helping monitor the malignant progression of LUAD and improve the prognosis of LUAD patients.
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http://dx.doi.org/10.3934/mbe.2021327DOI Listing
August 2021

Ultrafast laser surgery probe with a calcium fluoride miniaturized objective for bone ablation.

Biomed Opt Express 2021 Aug 12;12(8):4779-4794. Epub 2021 Jul 12.

The University of Texas at Austin, Department of Mechanical Engineering, 204 East Dean Keeton Street, Stop C2200, Austin, Texas 78712, USA.

We present a miniaturized ultrafast laser surgery probe with improved miniaturized optics to deliver higher peak powers and enable higher surgical speeds than previously possible. A custom-built miniaturized CaF objective showed no evidence of the strong multiphoton absorption observed in our previous ZnS-based probe, enabling higher laser power delivery to the tissue surface for ablation. A Kagome fiber delivered ultrashort pulses from a high repetition rate fiber laser to the objective, producing a focal beam radius of 1.96 μm and covering a 90×90 μm scan area. The probe delivered the maximum available fiber laser power, providing fluences >6 J/cm at the tissue surface at 53% transmission efficiency. We characterized the probe's performance through a parametric ablation study on bovine cortical bone and defined optimal operating parameters for surgery using an experimental- and simulation-based approach. The entire opto-mechanical system, enclosed within a 5-mm diameter housing with a 2.6-mm diameter probe tip, achieved material removal rates >0.1 mm/min, however removal rates were ultimately limited by the available laser power. Towards a next generation surgery probe, we simulated maximum material removal rates when using a higher power fiber laser and found that removal rates >2 mm/min could be attained through appropriate selection of laser surgery parameters. With future development, the device presented here can serve as a precise surgical tool with clinically viable speeds for delicate applications such as spinal decompression surgeries.
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http://dx.doi.org/10.1364/BOE.426149DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8407840PMC
August 2021

Ensemble Learning Based on Policy Optimization Neural Networks for Capability Assessment.

Sensors (Basel) 2021 Aug 28;21(17). Epub 2021 Aug 28.

School of Computer Science, Jilin University, No. 2699, Qianjing Rd., Changchun 130012, China.

Capability assessment plays a crucial role in the demonstration and construction of equipment. To improve the accuracy and stability of capability assessment, we study the neural network learning algorithms in the field of capability assessment and index sensitivity. Aiming at the problem of overfitting and parameter optimization in neural network learning, the paper proposes an improved machine learning algorithm-the Ensemble Learning Based on Policy Optimization Neural Networks (ELPONN) with the policy optimization and ensemble learning. This algorithm presents an optimized neural network learning algorithm through different strategies evolution, and builds an ensemble learning model of multi-intelligent algorithms to assess the capability and analyze the sensitivity of the indexes. Through the assessment of capabilities, the algorithm effectively avoids parameter optimization from entering the minimum point in performance to improve the accuracy of equipment capability assessment, which is significantly better than previous neural network assessment methods. The experimental results show that the mean relative error is 4.10%, which is better than BP, GABP, and early stopping. The ELPONN algorithm has better accuracy and stability performance, and meets the requirements of capability assessment.
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http://dx.doi.org/10.3390/s21175802DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8434125PMC
August 2021

Liquid metal coated copper micro-particles to construct core-shell structure and multiple heterojunctions for high-efficiency microwave absorption.

J Colloid Interface Sci 2021 Sep 1;607(Pt 1):210-218. Epub 2021 Sep 1.

Chongqing Key Laboratory of Soft-Matter Material Chemistry and Function Manufacturing, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China. Electronic address:

Facing the inherent defects of magnetic materials, the research of non-magnetic absorbers has gradually become a new direction in the research of microwave absorbers to fit the requirements of a new generation for high strength, wide effective absorption bandwidth. Herein, the liquid metal and copper (LC) composite micro-particles with multiple heterojunctions and core-shell structure, which have an excellent performance of microwave absorption (MA), were prepared by simply coating liquid metal on copper and then annealing. These special LC composite micro-particles exhibit excellent MA performance with the optimal reflection loss of -39.6 dB at thickness of 2.1 mm and a maximum effective absorption bandwidth of 4.96 GHz at thickness of 2.5 mm. The high MA performance of the LC composite particles are due to the enhancement of dielectric loss, including dipolar, interfacial, and dielectric polarization, which is caused by the special core-shell structure, multiple interfaces and heterojunctions. Furthermore, the multiple reflection/scattering of microwaves among particles or on the surface of particles also benefit to the high MA performance. Therefore, this study provides a facile method to construct multiple metal heterojunctions which have great prospects in microwave absorption applications.
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http://dx.doi.org/10.1016/j.jcis.2021.08.206DOI Listing
September 2021

Comparison of the Sedative and Analgesic Effects of Dexmedetomidine-Remifentanil and Dexmedetomidine-Sufentanil for Liposuction: A Prospective Single-Blind Randomized Controlled Study.

Aesthetic Plast Surg 2021 Sep 9. Epub 2021 Sep 9.

Department of Anesthesiology, National Center for Cardiovascular Diseases, Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: Dexmedetomidine had sedative and analgesic effects and did not produce significant respiratory depression at therapeutic doses.

Aims: To compare the sedative and analgesic effects and safety of dexmedetomidine combined with remifentanil or sufentanil in patients undergoing liposuction.

Methods: A total of 100 subjects were randomized 1:1 to two groups: Group R and Group S. First, patients were administered midazolam 0.02 mg·kg. Anesthesia was induced with an intravenous infusion of dexmedetomidine 1 µg kg (15 min) and remifentanil 0.1 µg kg min (Group R) or sufentanil 0.1 µg kgh (Group S). Anesthesia was maintained with an intravenous infusion of dexmedetomidine 1.0 µg kgh, midazolam 0.015 mg kgh, remifentanil 0.1 µg kgmin (Group R), or sufentanil 0.1 µg kgh (Group S). Hemodynamic and respiratory changes, modified OAA/S score and BIS values, postoperative Visual Analogue Scale pain scores, satisfaction of the patient and surgical team with the procedure, and adverse events and recovery time were recorded.

Results: Group R received significantly less midazolam and midazolam per hour compared to Group S (Group R vs. Group S: 3.4 ± 1.7 mg vs. 5.1 ± 2.0 mg, P < 0.0001; 1.5 ± 0.7 mg/h vs. 1.9 ± 0.6 mg/h, P = 0.002). The incidence of physical or verbal expressions of pain at the start of surgery was significantly lower in Group R compared to Group S (2 [4.3%] vs. 12 [26.7%], P = 0.003). Patient satisfaction with the procedure was significantly higher in Group R compared to Group S (3.9 ± 0.3 vs. 3.1 ± 0.3, P < 0.0001).

Conclusion: Dexmedetomidine-remifentanil and dexmedetomidine-sufentanil were effective and safe sedative and analgesic agents for liposuction. Hemodynamic stability was maintained. Dexmedetomidine-remifentanil might be associated with improved analgesic effects compared to dexmedetomidine-sufentanil.

Level Of Evidence Ii: Evidence was obtained from at least one properly designed randomized controlled trial. This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
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http://dx.doi.org/10.1007/s00266-021-02566-zDOI Listing
September 2021

Syngas Production via CO Reforming of Methane over Aluminum-Promoted NiO-10AlO-ZrO Catalyst.

ACS Omega 2021 Aug 18;6(34):22383-22394. Epub 2021 Aug 18.

College of Chemical Engineering, Sichuan University, Chengdu, Sichuan 610065, P. R. China.

CO reforming of methane was studied at medium temperature (700 °C) using a GSHV of 48,000 h over nickel catalysts supported on ZrO promoted by alumina. The catalysts were prepared by a one-step synthesis method and characterized by BET, H-TPR, XRD, XPS, TEM, Raman spectroscopy, and TGA. The NiO-10AlO-ZrO catalyst exhibited higher catalytic performance in comparison with the NiO-ZrO catalyst. The enhancement of catalytic activity in dry reforming could be associated with the alterations in surface properties due to Al promotion. First, the Al promoter could modify the structure of ZrO, leading to an increase of its pore volume and pore diameter. Second, the NiO-10AlO-ZrO catalyst exhibited high resistance to sintering. Third, the NiO-10AlO-ZrO catalyst showed high suppression to the loss of nickel during a long-term catalytic test. Finally, the addition of Al could inhibit the reduction of ZrO during the reduction and reaction, endowing further the stability.
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http://dx.doi.org/10.1021/acsomega.1c03174DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8412958PMC
August 2021

Effect of thoracic paravertebral block on intraoperative hypotension and postoperative pain in patients undergoing breast cancer surgery under general anesthesia: a retrospective study.

Ann Palliat Med 2021 Aug;10(8):8930-8938

Department of Anesthesiology, Wuxi 9th People's Hospital Affiliated to Soochow University, Wuxi, China.

Background: To retrospectively compare the effects of general anesthesia (GA) and thoracic paravertebral block (TPVB) combined with general anesthesia on the incidence of hypotension and postoperative pain in breast cancer (BC) surgery.

Methods: We retrospectively collected the medical records of patients who underwent BC surgery under general anesthesia from January 2018 to December 2020, and divided them into 2 groups according to the patient's anesthesia management method: GA group (Group G) and TPVB combined with GA group (Group T). During the operation, the use of boosting drugs and ephedrine, amount of fluid infusion, amount of bleeding, and operation time of the 2 participant groups were recorded, as well as the pain score in the resting state.

Results: During anesthesia, the bispectral index (BIS) value of Group G was significantly lower than that of Group T, the use of sufentanil and the use rate of ephedrine were significantly higher than that of Group T, and the difference was statistically significant (P<0.05). At the T4 time point, the blood pressure [systolic blood pressure/diastolic blood pressure (SBP/DBP)] of Group G was higher than that of Group T; at time point T3, the blood pressure (SBP/DBP) of Group G was lower than that of Group T. At the T4 time point, the heart rate of G group was higher than that of Group T, and the heart rate of G group was lower than that of Group T at the time points T2 and T3. The difference between the 2 groups was statistically significant. The change trend of the visual analogue scale (VAS) scores of the 2 participant groups was basically the same when they were resting peacefully, and there were statistical differences in the VAS scores at 1, 2, 4, and 8 h after surgery (P<0.05).

Conclusions: When TPVB is combined with GA, there is a lower incidence of hypotension, more stable circulatory state, and better postoperative analgesic effect.
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http://dx.doi.org/10.21037/apm-21-1803DOI Listing
August 2021

Case Report: Identification of Two Rare Fusions, and , That Coexist in a Lung Adenocarcinoma Patient and the Response to Alectinib.

Front Oncol 2021 13;11:722843. Epub 2021 Aug 13.

Department of Respiratory and Critical Care Medicine, West China Hospital, Sichuan University, Chengdu, China.

Several double fusions coexisting in one patient have been reported. However, few studies have reported the clinical efficacy of ALK inhibitors in rare double fusions. Here, we described a rare , double-fusion variant in a patient with metastatic lung adenocarcinoma. The patient responded well to alectinib (600 mg) twice daily. This case shows a promising treatment option for patients with rare double-fusion variants.
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http://dx.doi.org/10.3389/fonc.2021.722843DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8415000PMC
August 2021

A Multi-Sensor Mini-Bioreactor to Preselect Silage Inoculants by Tracking Metabolic Activity During Fermentation.

Front Microbiol 2021 12;12:673795. Epub 2021 Aug 12.

Department of Agricultural Engineering, University of Bonn, Bonn, Germany.

The microbiome in silage may vary substantially from the onset to the completion of fermentation. Improved additives and inoculants are being developed to accelerate the ensiling process, to enhance fermentation quality, and to delay spoilage during feed-out. However, current methods for preselecting and characterizing these amendments are time-consuming and costly. Here, we have developed a multi-sensor mini-bioreactor (MSMB) to track microbial fermentation and additionally presented a mathematical model for the optimal assessment among candidate inoculants based on the Bolza equation, a fundamental formula in optimal control theory. Three sensors [pH, CO, and ethanol (EtOH)] provided data for assessment, with four additional sensors (O, gas pressure, temperature, and atmospheric pressure) to monitor/control the fermentation environment. This advanced MSMB is demonstrated with an experimental method for evaluating three typical species of lactic acid bacteria (LAB), (LB) alone, and LB mixed with (LBLP) or with (LBEF), all cultured in De Man, Rogosa, and Sharpe (MRS) broth. The fermentation process was monitored over 48 h with these candidate microbial strains using the MSMB. The experimental results combine acidification characteristics with production of CO and EtOH, optimal assessment of the microbes, analysis of the metabolic sensitivity to pH, and partitioning of the contribution of each species to fermentation. These new data demonstrate that the MSMB associated with the novel rapid data-processing method may expedite development of microbial amendments for silage additives.
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http://dx.doi.org/10.3389/fmicb.2021.673795DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8406527PMC
August 2021

A dynamical study of SARS-COV-2: A study of third wave.

Results Phys 2021 Oct 22;29:104705. Epub 2021 Aug 22.

Department of Mathematics, College of Sciences, King Khalid University, Abha 61413, Saudi Arabia.

The coronavirus still an epidemic in most countries of the world and put the people in danger with so many infected cases and death. Considering the third wave of corona virus infection and to determine the peak of the infection curve, we suggest a new mathematical model with reported cases from March 06, 2021, till April 30, 2021. The model provides an accurate fitting to the suggested data, and the basic reproduction number calculated to be . We study the stability of the model and show that the model is locally as well as globally asymptotically stable when , for the disease free case. The parameters that are sensitive to the basic reproduction number, their effect on the model variables are shown graphically. We can observe that the suggested parameters can decrease efficiently the infection cases of the third wave in Pakistan. Further, our model suggests that the infection peak is to be May 06, 2021. The present results determine that the model can be useful in order to predict other countries data.
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http://dx.doi.org/10.1016/j.rinp.2021.104705DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8380310PMC
October 2021

SOX10 ablation severely impairs the generation of postmigratory neural crest from human pluripotent stem cells.

Cell Death Dis 2021 08 27;12(9):814. Epub 2021 Aug 27.

VIP Medical Service Center, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Animal studies have indicated that SOX10 is one of the key transcription factors regulating the proliferation, migration and differentiation of multipotent neural crest (NC), and mutation of SOX10 in humans may lead to type 4 Waardenburg syndrome (WS). However, the exact role of SOX10 in human NC development and the underlying molecular mechanisms of SOX10-related human diseases remain poorly understood due to the lack of appropriate human model systems. In this study, we successfully generated SOX10-knockout human induced pluripotent stem cells (SOX10 hiPSCs) by the CRISPR-Cas9 gene editing tool. We found that loss of SOX10 significantly inhibited the generation of p75HNK1/CD49D postmigratory neural crest stem cells (NCSCs) and upregulated the cell apoptosis rate during NC commitment from hiPSCs. Moreover, we discovered that both the neuronal and glial differentiation capacities of SOX10 NCSCs were severely compromised. Intriguingly, we showed that SOX10 hiPSCs generated markedly more TFAP2Cnonneural ectoderm cells (NNE) than control hiPSCs during neural crest differentiation. Our results indicate that SOX10 is crucial for the transition of premigratory cells to migrating NC and is vital for NC survival. Taken together, these results provide new insights into the function of SOX10 in human NC development, and the SOX10-knockout hiPSC lines may serve as a valuable cell model to study the pathogenesis of SOX10-related human neurocristopathies.
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http://dx.doi.org/10.1038/s41419-021-04099-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8397771PMC
August 2021

The impact of Helicobacter pylori infection, eradication therapy, and probiotics intervention on gastric microbiota in young adults.

Helicobacter 2021 Aug 26:e12848. Epub 2021 Aug 26.

Department of Gastroenterology, Peking University Third Hospital, Beijing, China.

Background: The impact of probiotics on non-Helicobacter pylori gastric microbiota and its role in microbial restoration after eradication were relatively unknown. We aimed to explore the effect of H. pylori eradication and probiotic intervention on gastric microbiota in young adults.

Methods: Fifty-six H. pylori-negative and 95 H. pylori-positive subjects aged 19-30 were included in this study. H. pylori-infected individuals were randomly assigned to quadruple therapy, probiotics supplemented quadruple therapy, or probiotics monotherapy group. Gastric mucosa and gastric juice samples were collected before and 2 months after treatment for 16SrRNA gene sequencing.

Results: The gastric microbial community structure and composition differed from H. pylori-negative subjects 2 months after successful H. pylori eradication. The α diversity of gastric mucosal microbiota significantly increased and was higher than H. pylori-negative subjects, while the α diversity of gastric juice microbiota decreased and was lower than the H. pylori-negative. After probiotics supplemented eradication treatment, Bifidobacterium was enriched in gastric mucosa, Lactobacillus was enriched in gastric juice, potentially pathogenic bacteria such as Fusobacterium and Campylobacter decreased, and the microbial diversity was closer to that of H. pylori-negative subjects compared to quadruple therapy group. Probiotics monotherapy significantly altered the diversity, community structure, and composition of gastric microbiota but showed no advantage in H. pylori inhibition and upregulating beneficial bacteria such as Bifidobacterium and Lactobacillus and related metabolism pathways. Certain potentially pathogenic bacteria such as Fusobacterium increased after probiotic monotherapy.

Conclusion: H. pylori eradication significantly disrupted gastric microbiota in young adults and could not be restored in a short time. Probiotics supplementation partially helped restore the gastric dysbiosis caused by eradication therapy, but it might be unnecessary for H. pylori-infected young adults to take probiotics alone.
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http://dx.doi.org/10.1111/hel.12848DOI Listing
August 2021

Opportunity Cost of Environmental Regulation in China's Industrial Sector.

Int J Environ Res Public Health 2021 08 13;18(16). Epub 2021 Aug 13.

Department of Public Policy, College of Liberal Arts and Social Sciences, City University of Hong Kong, Hong Kong, China.

In this paper, we employ a directional distance function to estimate the opportunity cost arising from environmental regulations in China's industrial sector. The change of opportunity cost is decomposed mathematically into two components including technical change and input change. Our results show that the opportunity cost attributed to environmental regulation is nil in some regions. The change of opportunity cost is marginal at the national level, as the positive effect of technical change is canceled out by the negative impact of input change on opportunity cost. Built on our mathematical decomposition, we further estimate the effects of environmental regulations on opportunity cost using a mediation model. It shows that environmental regulation has a significantly positive direct effect and a significantly negative indirect effect through foreign direct investment on opportunity cost. Our findings suggest, firstly, that inward FDI in China's industrial sector represents relatively dirty production technology; and, secondly, industrial production has transited towards a less carbon-intensive input mix. This paper, therefore, provides new insights for the recent dynamics of carbon abatement performance of China's industrial sector with policy implications.
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http://dx.doi.org/10.3390/ijerph18168579DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8392170PMC
August 2021
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