Publications by authors named "Ye Tian"

1,938 Publications

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Proteomic Analysis of Human Milk Reveals Nutritional and Immune Benefits in the Colostrum from Mothers with COVID-19.

Nutrients 2022 Jun 17;14(12). Epub 2022 Jun 17.

Institute for Cell Analysis, Shenzhen Bay Laboratory, Shenzhen 518132, China.

Despite the well-known benefits of breastfeeding and the World Health Organization's breastfeeding recommendations for COVID-19 infected mothers, whether these mothers should be encouraged to breastfeed is under debate due to concern about the risk of virus transmission and lack of evidence of breastmilk's protective effects against the virus. Here, we provide a molecular basis for the breastfeeding recommendation through mass spectrometry (MS)-based proteomics and glycosylation analysis of immune-related proteins in both colostrum and mature breastmilk collected from COVID-19 patients and healthy donors. The total protein amounts in the COVID-19 colostrum group were significantly higher than in the control group. While casein proteins in COVID-19 colostrum exhibited significantly lower abundances, immune-related proteins, especially whey proteins with antiviral properties against SARS-CoV-2, were upregulated. These proteins were detected with unique site-specific glycan structures and improved glycosylation diversity that are beneficial for recognizing epitopes and blocking viral entry. Such adaptive differences in milk from COVID-19 mothers tended to fade in mature milk from the same mothers one month postpartum. These results suggest that feeding infants colostrum from COVID-19 mothers confers both nutritional and immune benefits, and provide molecular-level insights that aid breastmilk feeding decisions in cases of active infection.
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http://dx.doi.org/10.3390/nu14122513DOI Listing
June 2022

Nonivamide induces brown fat-like characteristics in porcine subcutaneous adipocytes.

Biochem Biophys Res Commun 2022 Jun 16;619:68-75. Epub 2022 Jun 16.

College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address:

Obesity, which is associated with type 2 diabetes, is a threat to human health. There are studies, which suggest that some compounds can induce browning of white adipocytes to combat obesity. In this study, we selected nonivamide, an analog of capsaicin, to detect whether it influenced the browning of porcine white adipocytes. First, we found 25 μM nonivamide promoted apoptosis of porcine subcutaneous pre-adipocytes. After pre-adipocytes differentiation, nonivamide inhibited adipogenesis by reducing the expressions of Pparγ, Cebpα, while it promoted lipolysis by up-regulating Hsl, Atgl. Nonivamide also induced browning of porcine subcutaneous adipocytes by up-regulating the expression of brown and beige adipocyte gene markers, such as Prdm16, Cidea, and Slc27a1. Additionally, thermogenesis gene markers Cpt1a and Cpt1b were significantly up-regulated by nonivamide. Furthermore, nonivamide promoted mitochondrial biogenesis by up-regulating the expression of Tfam, Nrf1, Nrf2, and Tomm20. In conclusion, nonivamide is a potent compound to induce porcine adipocyte browning for treating obesity.
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http://dx.doi.org/10.1016/j.bbrc.2022.06.047DOI Listing
June 2022

Multi-Information Model for Large-Flowered Chrysanthemum Cultivar Recognition and Classification.

Front Plant Sci 2022 6;13:806711. Epub 2022 Jun 6.

Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, Beijing Laboratory of Urban and Rural Ecological Environment, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, National Engineering Research Center for Floriculture, School of Landscape Architecture, Beijing Forestry University, Beijing, China.

The traditional Chinese large-flowered chrysanthemum is one of the cultivar groups of chrysanthemum ( × Ramat.) with great morphological variation based on many cultivars. Some experts have established several large-flowered chrysanthemum classification systems by using the method of comparative morphology. However, for many cultivars, accurate recognition and classification are still a problem. Combined with the comparative morphological traits of selected samples, we proposed a multi-information model based on deep learning to recognize and classify large-flowered chrysanthemum. In this study, we collected the images of 213 large-flowered chrysanthemum cultivars in two consecutive years, 2018 and 2019. Based on the 2018 dataset, we constructed a multi-information classification model using non-pre-trained ResNet18 as the backbone network. The model achieves 70.62% top-5 test accuracy for the 2019 dataset. We explored the ability of image features to represent the characteristics of large-flowered chrysanthemum. The affinity propagation (AP) clustering shows that the features are sufficient to discriminate flower colors. The principal component analysis (PCA) shows the petal type has a better interpretation than the flower type. The training sample processing, model training scheme, and learning rate adjustment method affected the convergence and generalization of the model. The non-pre-trained model overcomes the problem of focusing on texture by ignoring colors with the ImageNet pre-trained model. These results lay a foundation for the automated recognition and classification of large-flowered chrysanthemum cultivars based on image classification.
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http://dx.doi.org/10.3389/fpls.2022.806711DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208330PMC
June 2022

Probiotic Consortia and Their Metabolites Ameliorate the Symptoms of Inflammatory Bowel Diseases in a Colitis Mouse Model.

Microbiol Spectr 2022 Jun 22:e0065722. Epub 2022 Jun 22.

School of Public Health, Xinxiang Medical University, Xinxiang, China.

Inflammatory bowel disease (IBD) has become a global public health problem. Although the pathogenesis of the disease is unknown, a potential association between the gut microbiota and inflammatory signatures has been established. Probiotics, especially or , are orally taken as food supplements or microbial drugs by patients with IBD or gastrointestinal disorders due to their safety, efficacy, and power to restore the gut microenvironment. In the current study, we investigated the comprehensive effects of probiotic bacterial consortia consisting of Lactobacillus reuteri, Lactobacillus gasseri, Lactobacillus acidophilus ( spp.), and Bifidobacterium lactis ( spp.) or their metabolites in a dextran sodium sulfate (DSS)-induced colitis mouse model. Our data demonstrate that probiotic consortia not only ameliorate the disease phenotype but also restore the composition and structure of the gut microbiota. Moreover, the effect of probiotic consortia is better than that of any single probiotic strain. The results also demonstrate that mixed fermentation metabolites are capable of ameliorating the symptoms of gut inflammation. However, the administration of metabolites is not as effective as probiotic consortia with respect to phenotypic characteristics, such as body weight, disease activity index (DAI), and histological score. In addition, mixed metabolites led only to changes in intestinal flora composition. In summary, probiotic consortia and metabolites could exert protective roles in the DSS-induced colitis mouse model by reducing inflammation and regulating microbial dysbiosis. These findings from the current study provide support for the development of probiotic-based microbial products as an alternative therapeutic strategy for IBD. IBD is a chronic nonspecific inflammatory disease. IBD is characterized by a wide range of lesions, often involving the entire colon, and is characterized mainly by ulcers and erosions of the colonic mucosa. In the present study, we investigated the efficacy of probiotics on the recovery of gut inflammation and the restoration of gut microecology. We demonstrate that probiotic consortia have a superior effect in inhibiting inflammation and accelerating recovery compared with the effects observed in the control group or groups administered with a single strain. These results support the utilization of probiotic consortia as an alternative therapeutic approach to treat IBD.
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http://dx.doi.org/10.1128/spectrum.00657-22DOI Listing
June 2022

Prognosis of patients with poorly differentiated gastric neuroendocrine neoplasms: a multi-center study in China.

Future Oncol 2022 Jun 22. Epub 2022 Jun 22.

Department of Geriatric gastroenterology, The First Affiliated Hospital of Nanjing Medical University, 210029.

The incidence of poorly differentiated gastric neuroendocrine neoplasms (G-NENs) has been increasing during the past decades. A total of 183 patients diagnosed with poorly differentiated G-NENs were enrolled from eight hospitals during 2010-2019 in China. All cases included have accepted abdominal surgery in tertiary hospitals. T3 (HR: 2.66, p = 0.019), T4 (HR: 3.62, p = 0.005), stage IV (HR: 5.67, p < 0.001), vascular invasion (HR: 1.59, p = 0.048) were independent risk factors for poor prognosis of poorly differentiated G-NENs. In stratified analysis, for patients with stage III tumors, those treated with chemotherapy had significantly longer survival than those accepting surgery alone. T3/T4 stage, TNM stage IV and vascular invasion were independent negative prognostic factors for patients with poorly differentiated G-NENs. Patients with stage III tumors can benefit from chemotherapy. Highly selected patients with stage IV tumors may also benefit from surgery.
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http://dx.doi.org/10.2217/fon-2022-0140DOI Listing
June 2022

Final Overall Survival Analysis of Gemcitabine and Cisplatin Induction Chemotherapy in Nasopharyngeal Carcinoma: A Multicenter, Randomized Phase III Trial.

J Clin Oncol 2022 Jun 16:JCO2200327. Epub 2022 Jun 16.

Department of Head and Neck Oncology, The Cancer Center of the Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, China.

JCO We previously reported significantly improved failure-free survival using gemcitabine plus cisplatin induction chemotherapy in locoregionally advanced nasopharyngeal carcinoma. Here, we present the final overall survival (OS) analysis. In this multicenter, randomized trial, patients were assigned to be treated with concurrent chemoradiotherapy alone (standard therapy, n = 238) or gemcitabine and cisplatin induction chemotherapy before concurrent chemoradiotherapy (n = 242). With a median follow-up of 69.8 months, the induction chemotherapy group had a significantly higher 5-year OS (87.9% 78.8%, hazard ratio, 0.51 [95% CI 0.34 to 0.78]; = .001) and a comparable risk of late toxicities (≥ grade 3, 11.3% 11.4%). Notably, the depth of the tumor response to induction chemotherapy correlated significantly and positively with survival (complete response partial response stable/progressive disease, 5-year OS, 100% 88.4% 61.5%, = .005). Besides, patients with a low pretreatment cell-free Epstein-Barr virus DNA load (< 4,000 copies/mL) might not benefit from induction chemotherapy (5-year OS, 90.6% 91.4%, = .77). In conclusion, induction chemotherapy before concurrent chemoradiotherapy improved OS significantly in patients with locally advanced nasopharyngeal carcinoma, without increasing the risk of late toxicities. Tumor response to induction chemotherapy and pretreatment cell-free Epstein-Barr virus DNA might be useful to guide individualized treatment.
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http://dx.doi.org/10.1200/JCO.22.00327DOI Listing
June 2022

Protein disulfide isomerase PDI-6 regulates Wnt secretion to coordinate inter-tissue UPR activation and lifespan extension in C. elegans.

Cell Rep 2022 Jun;39(10):110931

State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100093, China; Center for Excellence in Animal Evolution and Genetics, Chinese Academy of Sciences, Kunming 650223, China. Electronic address:

Coordination of inter-tissue stress signaling is essential for organismal fitness. Neuronal mitochondrial perturbations activate the mitochondrial unfolded-protein response (UPR) in the intestine via the mitokine Wnt signaling in Caenorhabditis elegans. Here, we found that the protein disulfide isomerase PDI-6 coordinates inter-tissue UPR signaling via regulating the Wnt ligand EGL-20. PDI-6 is expressed in the endoplasmic reticulum (ER) and interacts with EGL-20 through disulfide bonds that are essential for EGL-20 stability and secretion. pdi-6 deficiency results in misfolded EGL-20, which leads to its degradation via ER-associated protein degradation (ERAD) machinery. Expression of PDI-6 declines drastically with aging, and animals with pdi-6 deficiency have decreased lifespan. Overexpression of PDI-6 is sufficient to maintain Wnt/EGL-20 protein levels during aging, activating the UPR, and significantly extending lifespan in a Wnt- and UPR-dependent manner. Our study reveals that protein disulfide isomerase facilitates Wnt secretion to coordinate the inter-tissue UPR signaling and organismal aging.
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http://dx.doi.org/10.1016/j.celrep.2022.110931DOI Listing
June 2022

Ticagrelor versus clopidogrel in reducing inflammatory cell infiltration of thrombus aspirated in patients with ST-elevation myocardial infarction.

Eur J Clin Pharmacol 2022 Jun 8. Epub 2022 Jun 8.

Department of Cardiology, the First Affiliated Hospital, Cardiovascular Institute, Harbin Medical University, Harbin, 150001, People's Republic of China.

Background: Ticagrelor provides more rapid, potent, and consistent anti-platelet efficacy than clopidogrel. This randomized trial aimed to evaluate the anti-inflammation effects of ticagrelor versus clopidogrel on thrombus aspirated from the ST-elevation myocardial infarction (STEMI) patients.

Method: A total of 98 patients with STEMI and intended percutaneous coronary intervention (PCI) were randomly assigned to receive clopidogrel (600-mg loading dose) or ticagrelor (180-mg loading dose), of whom 55 with large thrombus burden underwent thrombus aspiration during PCI. Thrombus specimens were successfully aspirated from 49 patients. Finally, 24 patients in the clopidogrel group and 23 in the ticagrelor group completed the study. Inflammatory cells within thrombi were assessed by hematoxylin-eosin and immunohistochemistry stainings.

Results: Compared with the clopidogrel group, the number of total inflammatory cells per mm thrombus area in the ticagrelor group was decreased by 28% (P = 0.009). The numbers of neutrophils and myeloperoxidase-positive cells per mm thrombus area in the ticagrelor group were respectively decreased by 35% (P = 0.016) and 28% (P = 0.047), as compared with those in the clopidogrel group. Moreover, ticagrelor treatment reduced the ratio of monocytes number higher than 250 per mm thrombus area compared with clopidogrel treatment (4% versus 29%, P = 0.048).

Conclusion: In patients with undergoing PCI for STEMI, the loading dose ticagrelor regimen was associated with a reduction in inflammatory cell infiltration within thrombus compared with the loading dose clopidogrel regimen.
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http://dx.doi.org/10.1007/s00228-022-03348-5DOI Listing
June 2022

The relationship between prostatic microvessel density and different concentrations of oestrogen/androgen in Sprague-Dawley rats.

Eur J Med Res 2022 Jun 7;27(1):87. Epub 2022 Jun 7.

Department of Urology, Guizhou Provincial People's Hospital, Guiyang, Guizhou, People's Republic of China.

Background: Currently, there are relatively few studies on the effects of changes in oestrogen and androgen levels on prostatic microvessel density (MVD). This article aimed to study the changes in prostatic MVD in Sprague-Dawley (SD) rats after castration under the effect of oestrogen/androgen at different concentrations.

Methods: Male SD rats aged 3-4 months were randomly divided into a control group, a castration group, and groups with different concentrations of oestrogen/androgen treatment after castration. Dihydrotestosterone (DHT) and oestradiol (E) were administered daily by subcutaneous injection for one month. All the rats were killed by cervical dislocation after one month, and the serum DHT and E concentrations of the rats in each group were measured by ELISA. Prostate tissue specimens were immunohistochemically stained with monoclonal antibodies against CD34 and factor VIII for MVD.

Results: Compared with the control group, the MVD decreased significantly in the castration group (P < 0.05). When the exogenous E concentration was constant, in general, the MVD of rats in all the groups increased with increasing exogenous DHT concentration. Compared with the castration group, the MVD increased significantly in the E0.05 + DHT0.015 mg/kg, E0.05 + DHT0.05 mg/kg, E0.05 + DHT0.15 mg/kg, E0.05 + DHT0.5 mg/kg, and E0.05 + DHT1.5 mg/kg groups (P < 0.05). In addition, when the exogenous DHT concentration was constant, the MVD increased with increasing exogenous E concentration in all the groups. Among them, compared with the control and castration groups, the MVD increased significantly in the DHT0.15 + E0.015 mg/kg, DHT0.15 + E0.15 mg/kg, and DHT0.15 + E0.5 mg/kg groups (P < 0.05).

Conclusions: Androgens play an important role in the regulation of prostatic MVD in SD rats, and a decrease in DHT concentration can induce a decrease in prostatic MVD. In contrast, prostatic MVD can be increased with increasing DHT concentration. In addition, prostatic MVD can be increased gradually with increasing oestrogen concentration.
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http://dx.doi.org/10.1186/s40001-022-00719-7DOI Listing
June 2022

Estrogen and G protein-coupled estrogen receptor accelerate the progression of benign prostatic hyperplasia by inducing prostatic fibrosis.

Cell Death Dis 2022 Jun 7;13(6):533. Epub 2022 Jun 7.

Department of Urology, Peking University First Hospital, 100034, Beijing, China.

Benign prostatic hyperplasia (BPH) is the most common and progressive urological disease in elderly men worldwide. Epidemiological studies have suggested that the speed of disease progression varies among individuals, while the pathophysiological mechanisms of accelerated clinical progression in some BPH patients remain to be elucidated. In this study, we defined patients with BPH as belonging to the accelerated progressive group (transurethral resection of the prostate [TURP] surgery at ≤50 years old), normal-speed progressive group (TURP surgery at ≥70 years old), or non-progressive group (age ≤50 years old without BPH-related surgery). We enrolled prostate specimens from the three groups of patients and compared these tissues to determine the histopathological characteristics and molecular mechanisms underlying BPH patients with accelerated progression. We found that the main histopathological characteristics of accelerated progressive BPH tissues were increased stromal components and prostatic fibrosis, which were accompanied by higher myofibroblast accumulation and collagen deposition. Mechanism dissection demonstrated that these accelerated progressive BPH tissues have higher expression of the CYP19 and G protein-coupled estrogen receptor (GPER) with higher estrogen biosynthesis. Estrogen functions via GPER/Gαi signaling to modulate the EGFR/ERK and HIF-1α/TGF-β1 signaling to increase prostatic stromal cell proliferation and prostatic stromal fibrosis. The increased stromal components and prostatic fibrosis may accelerate the clinical progression of BPH. Targeting this newly identified CYP19/estrogen/GPER/Gαi signaling axis may facilitate the development of novel personalized therapeutics to better suppress the progression of BPH.
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http://dx.doi.org/10.1038/s41419-022-04979-3DOI Listing
June 2022

Learning curves of flexible ureteroscopy (275 cases) and prone percutaneous nephrolithotomy (73 cases) in paediatric stones: data from 348 children.

J Endourol 2022 Jun 7. Epub 2022 Jun 7.

Capital Medical University Affiliated Beijing Friendship Hospital, 26455, No. 95, Yongan Road, Xicheng District, Beijing, China., Beijing, China, 100050;

Objectives: To evaluate learning curves in pediatric flexible ureteroscopy (FURS) and pediatric prone percutaneous nephrolithotomy (PCNL) by a single surgeon with experience in adult endourological procedures.

Materials And Methods: Children who were diagnosed with nephrolithiasis and treated with PCNL or FURS from June 2014 to April 2019 were analyzed. Patient demographics, stone characteristics, stone-free rate (SFR), complication rate (CR) were reported. Learning curves were generated to estimate the effect of a surgeon's experience on outcomes.

Results: Seventy-three children underwent PCNL on 86 sides in 77 operations and 275 children underwent FURS on 320 sides in 288 operations. The SFRs were 88.1% (282/320) for FURS procedures and 89.5% (77/86) for PCNL procedures. CRs were 19.8% (57/288) and 35.1% (27/77), respectively. Learning curves showed that the SFRs of the two procedures increased with the accumulation of cases. There was an apparent improvement of SFR for PCNL procedures after about 60 surgeries. A favourable SFR of FURS could be achieved at the start of learning. No apparently decreased CRs were observed for either PCNL or FURS.

Conclusions: Both PCNL and FURS could achieve satisfactory SFRs and accepted CRs in paediatric stones. Increased surgical experience was associated with improved SFRs of both PCNL and FURS procedures and the surgeon's adult FURS experience translated to paediatric FURS better than adult PCNL experience translated to the paediatric PCNL. A surgeon needs approximate 60 cases of PCNL to achieve competence. For FURS, a favourable SFR could be achieved at the start of learning.
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http://dx.doi.org/10.1089/end.2021.0757DOI Listing
June 2022

The Multi-Institutional Experience in Single-Port Robotic Transvesical Simple Prostatectomy for Benign Prostatic Hyperplasia Management. Letter.

J Urol 2022 Jun 3:101097JU0000000000002784. Epub 2022 Jun 3.

Department of Urology, Guizhou Provincial People's Hospital, Guiyang, China.

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http://dx.doi.org/10.1097/JU.0000000000002784DOI Listing
June 2022

Characterization and Performance Testing of an Intumescent Nanoinhibitor for Inhibiting Coal Spontaneous Combustion.

ACS Omega 2022 May 11;7(20):17202-17214. Epub 2022 May 11.

State Key Laboratory of High-Efficient Mining and Safety of Metal Mines (University of Science and Technology Beijing), Ministry of Education, Beijing 100083, China.

Considering disadvantages such as the low thermal stability and environmental pollution of existing gel inhibitors, a green and stable intumescent nanoinhibitor (INI) was prepared and tested. First, polyacrylamide (PAM), nano-silica, and intumescent flame retardant (IFR) were selected as raw materials. The INI was prepared by nanoparticle modification and cross-linking polymerization. Then, the structure and physical properties of INI were tested by Fourier transform infrared spectroscopy, scanning electron microscopy, and rheological experiments. Meanwhile, the inhibition performance of INI was studied through thermogravimetric analysis-Fourier transfer infrared spectroscopy (TGA-FTIR) analysis. The results suggest that the nanomodification improved the dispersibility of INI particles. The addition of modified nano-silica (MNS) and IFR enhances the strength of the reticular structure, thereby improving the transport convenience and covering ability of the INI gel. At high temperatures, IFR can generate a porous foamed carbon layer that further coats the coal. After INI inhibition treatment, the characteristic temperature and activation energy of coal were significantly improved, and the production of carbon monoxide and carbon dioxide decreased. Hence, irrespective of physical properties, physical inhibition performance, or chemical inhibition performance, INI performed well. Research results can provide valuable references for the preparation and performance study of a coal spontaneous combustion inhibitor.
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http://dx.doi.org/10.1021/acsomega.2c00998DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9134418PMC
May 2022

Corrigendum: Menthol Flavor in E-Cigarette Vapor Modulates Social Behavior Correlated With Central and Peripheral Changes of Immunometabolic Signalings.

Front Mol Neurosci 2022 12;15:913285. Epub 2022 May 12.

Guangdong Provincial Key Laboratory of Brain Connectome and Behavior, CAS Key Laboratory of Brain Connectome and Manipulation, Brain Cognition and Brain Disease Institute (BCBDI), Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.

[This corrects the article DOI: 10.3389/fnmol.2022.800406.].
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http://dx.doi.org/10.3389/fnmol.2022.913285DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9135120PMC
May 2022

The P.R.OS.T.A.T.E Nomogram for the Preoperative Prediction of Clinical Efficacy of Transurethral Resection of the Prostate in Benign Prostatic Hyperplasia Patients.

Clin Interv Aging 2022 23;17:845-855. Epub 2022 May 23.

Department of Urology, Guizhou Provincial People's Hospital, Guiyang, People's Republic of China.

Purpose: Transurethral resection of the prostate (TURP) is often indicated for benign prostatic hyperplasia (BPH). Some patients, however, fail to adequately respond to these interventions. Accordingly, a powerful prediction model for TURP efficacy is warranted. This study aimed to create a nomogram with preoperative parameters for the prediction of individual TURP efficacy.

Methods: Clinical data from 356 BPH subjects who underwent TURP were retrospectively collected between November 2015 and June 2021 for nomogram development. The prediction model was developed using multivariable logistic regression analysis and presented as a nomogram. Nomogram performance was assessed through calibration curves and the concordance index (C-index). An independent validation cohort containing 177 consecutive patients in the corresponding period was used for external validation. The optimal cutoff value was determined through receiver operating characteristic curve (ROC) analysis by maximizing the Youden index, and its accuracy was assessed through sensitivity, specificity and predictive values.

Results: In multivariate analysis of the primary cohort, the independent factors for TURP efficacy were age, International Prostate Symptom Score (IPSS), intravesical prostatic protrusion (IPP), bladder wall thickness (BWT), peripheral zone thickness (PT) and transitional zone thickness (TT), all of which were included in the nomogram. The calibration curve for survival probability showed good agreement between the nomogram predictions and actual observations. The C-index for predicting TURP efficacy was 0.860 (95% confidence interval [CI], 0.808-0.911). The optimal cutoff total nomogram score was 177, with a maximum Youden index of 0.643. The sensitivity, specificity, positive predictive value, and negative predictive value for predicting TURP efficacy were 70.6%, 75.6%, 90.6%, and 43.7% in the validation cohort, respectively. Logistic regression analysis in the validation cohort demonstrated that the area under the curve (AUC) was 0.806 (95% CI, 0.733-0.879).

Conclusion: The P.R.OS.T.A.T.E nomogram objectively and accurately predicted TURP efficacy, thereby facilitating the clinical decision-making process.
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http://dx.doi.org/10.2147/CIA.S365282DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138692PMC
June 2022

Multi-Signal Detection Framework: A Deep Learning Based Carrier Frequency and Bandwidth Estimation.

Sensors (Basel) 2022 May 21;22(10). Epub 2022 May 21.

National Space Science Center, Chinese Academy of Sciences, Beijing 100190, China.

Multi-signal detection is of great significance in civil and military fields, such as cognitive radio (CR), spectrum monitoring, and signal reconnaissance, which refers to jointly detecting the presence of multiple signals in the observed frequency band, as well as estimating their carrier frequencies and bandwidths. In this work, a deep learning-based framework named SigdetNet is proposed, which takes the power spectrum as the network's input to localize the spectral locations of the signals. In the proposed framework, Welch's periodogram is applied to reduce the variance in the power spectral density (PSD), followed by logarithmic transformation for signal enhancement. In particular, an encoder-decoder network with the embedding pyramid pooling module is constructed, aiming to extract multi-scale features relevant to signal detection. The influence of the frequency resolution, network architecture, and loss function on the detection performance is investigated. Extensive simulations are carried out to demonstrate that the proposed multi-signal detection method can achieve better performance than the other benchmark schemes.
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http://dx.doi.org/10.3390/s22103909DOI Listing
May 2022

2D-DOA Estimation in Switching UCA Using Deep Learning-Based Covariance Matrix Completion.

Sensors (Basel) 2022 May 14;22(10). Epub 2022 May 14.

National Space Science Center, Chinese Academy of Sciences, Beijing 100190, China.

In this paper, we study the two-dimensional direction of arrival (2D-DOA) estimation problem in a switching uniform circular array (SUCA), which means performing 2D-DOA estimation with a reduction in the number of radio frequency (RF) chains. We propose a covariance matrix completion algorithm for 2D-DOA estimation in a SUCA. The proposed algorithm estimates the complete covariance matrix of a fully sampled UCA (FUCA) from the sample covariance matrix of the SUCA through a neural network. Afterwards, the MUSIC algorithm is performed for 2D-DOA estimation with the completed covariance matrix. We conduct Monte Carlo simulations to evaluate the performance of the proposed algorithm in various scenarios; the performance of 2D-DOA estimation in the SUCA gradually approaches that in the FUCA as the SNR or the number of snapshots increases, which means that the advantages of a FUCA can be preserved with fewer RF chains. In addition, the proposed algorithm is able to implement underdetermined 2D-DOA estimation.
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http://dx.doi.org/10.3390/s22103754DOI Listing
May 2022

The Cause of Ribbon Fluctuation in Magnetorheological Finishing and Its Influence on Surface Mid-Spatial Frequency Error.

Micromachines (Basel) 2022 Apr 29;13(5). Epub 2022 Apr 29.

College of Intelligence Science and Technology, National University of Defense Technology, 109 Deya Road, Changsha 410073, China.

In the high-power laser system, the mid-spatial frequency error of the surface of the high-power laser component will affect the normal operation of the high-power laser system. In order to improve the mid-spatial frequency error of the high-power laser component after magnetorheological finishing, the causes and influencing factors of the ribbon fluctuation in magnetorheological finishing are studied, and the influence of different ribbon fluctuation on the mid-spatial frequency error of the surface is studied. Firstly, the influence of different ribbon fluctuations on the mid-spatial frequency error of the machined surface is simulated by a computer. Secondly, the magnetic field in the circumferential direction of the polishing wheel, the fluctuation amount and frequency of the magnetorheological polishing ribbon are measured, and then the causes of the fluctuation of the magnetorheological polishing ribbon are analyzed. Moreover, through the principle of a single variable, the influence of process parameters on the fluctuation of magnetorheological polishing ribbon is explored. Finally, the fused silica component is scanned uniformly under the process parameters of magnetorheological polishing ribbon fluctuation of 40 μm, 80 μm, 150 μm, and 200 μm. The experimental results show that the greater the ribbon fluctuation, the greater the surface mid-spatial frequency error of the component, and the ribbon fluctuation is approximately linear with the RMS of the PSD2 in the mid-spatial frequency band on the surface of the component. Therefore, the fluctuation of the ribbon can be controlled by controlling the magnetorheological processing parameters, and the mid-spatial frequency band error on the surface of the high-power laser component can be significantly reduced by optimizing process parameters after magnetorheological finishing.
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http://dx.doi.org/10.3390/mi13050697DOI Listing
April 2022

Nearly Lattice-Matched GaN Distributed Bragg Reflectors with Enhanced Performance.

Materials (Basel) 2022 May 14;15(10). Epub 2022 May 14.

Department of Electronic and Electrical Engineering, The University of Sheffield, Sheffield S1 3JD, UK.

Heavy silicon-doping in GaN generally causes a rough surface and saturated conductivity, while heavily silicon-doped -AlGaN with ≤5% aluminum can maintain an atomically flat surface and exhibit enhanced conductivity. Given this major advantage, we propose using multiple pairs of heavily silicon-doped -AlGaN and undoped GaN instead of widely used multiple pairs of heavily silicon-doped -GaN and undoped GaN for the fabrication of a lattice-matched distributed Bragg reflector (DBR) by using an electrochemical (EC) etching technique, where the lattice mismatch between AlGaN and GaN can be safely ignored. By means of using the EC etching technique, the -layers can be converted into nanoporous (NP) layers whilst the undoped GaN remains intact, leading to a significantly high contrast in refractive index between NP-layer and undoped GaN and thus forming a DBR. Our work demonstrates that the NP-AlGaN/undoped GaN-based DBR exhibits a much smoother surface, enhanced reflectivity and a wider stopband than the NP-GaN/undoped GaN-based DBR. Furthermore, the NP-AlGaN/undoped GaN-based DBR sample with a large size (up to 1 mm in width) can be obtained, while a standard NP-GaN/undoped GaN-based DBR sample obtained is typically on a scale of a few 100 μm in width. Finally, a series of DBR structures with high performance, ranging from blue to dark yellow, was demonstrated by using multiple pairs of -AlGaN and undoped GaN.
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http://dx.doi.org/10.3390/ma15103536DOI Listing
May 2022

A biodegradable 3D woven magnesium-based scaffold for orthopedic implants.

Biofabrication 2022 Jun 13;14(3). Epub 2022 Jun 13.

Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD, United States of America.

Porous Magnesium (Mg) is a promising biodegradable scaffold for treating critical-size bone defects, and as an essential element for human metabolism, Mg has shown sufficient biocompatibility. Its elastic moduli and yield strengths are closer to those of cortical bone than common, inert metallic implants, effectively reducing stress concentrations around host tissue as well as stress shielding. More importantly, Mg can degrade and be absorbed in the human body in a safe and controlled manner, thereby reducing the need for second surgeries to remove implants. The development of porous Mg scaffolds via conventional selective laser melting techniques has been limited due to Mg's low boiling point, high vapor pressures, high reactivity, and non-ideal microstructures in additively manufactured parts. Here we present an exciting alternative to conventional additive techniques: 3D weaving with Mg wires that have controlled chemistries and microstructures. The weaving process offers high throughput manufacturing as well as porous architectures that can be optimized for stiffness and porosity with topology optimization. Once woven, we dip-coat the weaves with polylactic acid to enhance their strength and corrosion resistance. Following fabrication, we characterize their mechanical properties, corrosion behavior, and cell compatibility, and we use an intramuscular implantation model to evaluate theircorrosion behavior and tissue response.
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http://dx.doi.org/10.1088/1758-5090/ac73b8DOI Listing
June 2022

Percutaneous transluminal forceps biopsy with an adjustable curved sheath for obstructive jaundice.

Minim Invasive Ther Allied Technol 2022 May 26:1-7. Epub 2022 May 26.

Department of Interventional Treatment, First Hospital of Qinhuangdao, Qinhuangdao, Hebei Province, P. R. China.

Purpose: To evaluate the feasibility and safety of percutaneous transluminal forceps biopsy (PTFB) with an adjustable curved sheath in patients with obstructive jaundice.

Material And Methods: Forty-two patients who underwent PTFB with an adjustable curved sheath were analyzed retrospectively. Sensitivity, specificity, negative predictive value (NPV), positive predictive value (PPV) and accuracy were calculated for all populations and in different situations. Technical success and safety were evaluated.

Results: The technical success rate was 100%. Thirty-five of 42 cases were diagnosed malignant diseases, the sensitivity of PTFB with an adjustable curved sheath was 74.29% (26/35), the specificity was 100%, the positive predictive value was 100%, the negative predictive value was 43.75% (7/16), and the accuracy rate was 78.57% (33/42). There was a better sensitivity for bile duct malignancies when compared with non-bile duct malignancies ( = 0.012). No statistical difference was found in the sensitivity of the upper part of the biliary tree and the lower part of the biliary tree, and none in the sensitivity of different approaches (left vs. right). The complication rate was 11.90%, and no serious complications were observed.

Conclusions: PTFB with an adjustable curved sheath is an effective and safe technique, without being limited by approaches and obstruction sites.
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http://dx.doi.org/10.1080/13645706.2022.2079381DOI Listing
May 2022

Anatomical limitations and factors influencing molar distalization.

Angle Orthod 2022 May 23. Epub 2022 May 23.

Objectives: To analyze the anatomical limitations and characteristics of maxillary and mandibular retromolar regions affecting molar distalization using cone-beam computed tomography (CBCT).

Materials And Methods: A total of 120 qualifying patients were classified into equal groups of skeletal Class II and Class III and stratified by vertical growth pattern, age, sex, and third molar presence. The available distance along the axis of distalization and cortical bone thickness (CBT) were measured in the maxillary and mandibular retromolar regions of Class II and Class III patients, respectively. One-way analysis of variance was used to examine the effects of the factors on the measured data.

Results: The minimum available distance of the Class II maxilla was observed at a level 3 mm from the cementoenamel junction (CEJ), while that of the Class III mandible was at a level 9 mm from the CEJ. The average available distance at the limit level was 4.06 ± 1.93 mm in the Class II maxilla, and the average corresponding CBT was 1.00 mm. The average available distance at the limit level in the Class III mandible was 2.80 ± 1.96 mm, and the corresponding CBT was 2.24 mm. In both skeletal Class II and Class III patients, hyperdivergent groups had the least available distance for molar distalization.

Conclusions: The limit for available distance in the Class II maxilla is closer to the coronal level, while that of the Class III mandible is closer to the apical level. A hyperdivergent growth pattern in a patient is indicative of less potential for molar distalization. Axial slices of CBCT images provide valuable evaluation for molar distalization regarding limit levels.
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http://dx.doi.org/10.2319/092921-731.1DOI Listing
May 2022

Synthesis and biological evaluation of new series of benzamide derivatives containing urea moiety as sEH inhibitors.

Bioorg Med Chem Lett 2022 Aug 19;70:128805. Epub 2022 May 19.

Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, PR China. Electronic address:

The pharmacological inhibition of soluble epoxide hydrolase (sEH) was shown to reduce inflammation and pain. Herein, we described a series of newly synthesized sEH inhibitors with the trident-shaped skeleton. Intensive structural modifications led to the identification of compound B15 as a potent sEH inhibitor with an IC value of 0.03 ± 0.01 nM. Furthermore, compound B15 showed satisfactory metabolic stability in human liver microsomes with a half-time of 197 min. In carrageenan-induced inflammatory pain rat model, compound B15 exhibited a better therapeutic effect compared to t-AUCB and Celecoxib, which demonstrated the proof of potential as anti-inflammatory agents for pain relief.
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http://dx.doi.org/10.1016/j.bmcl.2022.128805DOI Listing
August 2022

DNA-Mediated Assembly of Carbon Nanomaterials.

Chempluschem 2022 Apr 26;87(5):e202200089. Epub 2022 Apr 26.

College of Engineering and Applied Sciences, State Key Laboratory of Analytical Chemistry for Life Science, Jiangsu Key Laboratory of Artificial Functional Materials, Chemistry and Biomedicine Innovation Center, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, 210023, Nanjing, P. R. China.

Carbon nanomaterials (CNMs) have attracted extensive attentions on account of their superior electrical, mechanical, optical, and biological properties. However, the dimensional limit and irregular arrangement have hampered their further application. It is necessary to find an easy, efficient and controllable way to assemble CNMs into well-ordered array. DNA nanotechnology, owning to the advantages of precise programmability, highly structural predictability and spatial addressability, has been widely applied in the assembly of CNMs. Summarizing the progress and achievements in this field will be of great value to related studies. Herein, based on the different dimensions of CNMs containing 0-dimensional (0D) carbon dots (CDs), fullerenes, 1-dimensional (1D) carbon nanotubes (CNTs) and 2-dimensional (2D) graphene, we introduced the conjugation strategies between DNA and CNMs, their different assembly methods and their applications. In addition, we also discuss the existing challenges and future opportunities in the field.
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http://dx.doi.org/10.1002/cplu.202200089DOI Listing
April 2022

Microfluidic Platform for Time-Resolved Characterization of Protein Higher-Order Structures and Dynamics Using Top-Down Mass Spectrometry.

Anal Chem 2022 May 18;94(21):7520-7527. Epub 2022 May 18.

Research Center for Biomedical Optics and Molecular Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.

Characterization of protein higher-order structures and dynamics is essential for understanding the biological functions of proteins and revealing the underlying mechanisms. Top-down mass spectrometry (MS) accesses structural information at both the intact protein level and the peptide fragment level. Native top-down MS allows analysis of a protein complex's architecture and subunits' identity and modifications. Top-down hydrogen/deuterium exchange (HDX) MS offers high spatial resolution for conformational or binding interface analysis and enables conformer-specific characterization. A microfluidic chip can provide superior performance for front-end reactions useful for these MS workflows, such as flexibility in manipulating multiple reactant flows, integrating various functional modules, and automation. However, most microchip-MS devices are designed for bottom-up approaches or top-down proteomics. Here, we demonstrate a strategy for designing a microchip for top-down MS analysis of protein higher-order structures and dynamics. It is suitable for time-resolved native MS and HDX MS, with designs aiming for efficient ionization of intact protein complexes, flexible manipulation of multiple reactant flows, and precise control of reaction times over a broad range of flow rates on the submicroliter per minute scale. The performance of the prototype device is demonstrated by measurements of systems including monoclonal antibodies, antibody-antigen complexes, and coexisting protein conformers. This strategy may benefit elaborate structural analysis of biomacromolecules and inspire method development using the microchip-MS approach.
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http://dx.doi.org/10.1021/acs.analchem.2c00077DOI Listing
May 2022

Psoralidin protects against cerebral hypoxia/reoxygenation injury: Role of GAS6/Axl signaling.

Phytother Res 2022 Jun 18;36(6):2628-2640. Epub 2022 May 18.

Xi'an Key Laboratory of Cardiovascular and Cerebrovascular Diseases, Xi'an No. 3 Hospital, The Affiliated Hospital of Northwest University, School of Life Sciences and Medicine, Northwest University, Xi'an, China.

Psoralidin (PSO) is a natural phenolic coumarin extracted from the seeds of Psoralea corylifolia L. Growing preclinical evidence indicates that PSO has anti-inflammatory, anti-vitiligo, anti-bacterial, and anti-viral effects. Growth arrest-specific gene 6 (GAS6) and its receptor, Axl, modulate cellular oxidative stress, apoptosis, survival, proliferation, migration, and mitogenesis. Notably, the neuroprotective role of the GAS6/Axl axis has been identified in previous studies. We hypothesize that PSO ameliorates cerebral hypoxia/reoxygenation (HR) injury via activating the GAS6/Axl signaling. We first confirmed that PSO was not toxic to the cells and upregulated GAS6 and Axl expression after HR injury. Moreover, PSO exerted a marked neuroprotective effect against HR injury, represented by restored cell viability and cell morphology, decreased lactate dehydrogenase (LDH) release, and reactive oxygen species (ROS) generation. Furthermore, PSO pretreatment also elevated the levels of nuclear factor-related factor 2 (Nrf-2), NAD(P)H dehydrogenase quinone-1 (NQO1), heme oxygenase-1 (HO-1), silent information regulator 1 (SIRT1), peroxisome proliferator-activated receptor coactivator 1α (PGC-1α), nuclear respiratory factor 1 (NRF1), uncoupling protein 2 (UCP2), and B-cell lymphoma 2 (BCl2) both in the condition of baseline and HR injury. However, GAS6 siRNA or Axl siRNA inhibited the neuroprotective effects of PSO. Our findings suggest that PSO pretreatment attenuated HR-induced oxidative stress, apoptosis, and mitochondrial dysfunction in neuroblastoma cells through the activation of GAS6/Axl signaling.
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http://dx.doi.org/10.1002/ptr.7481DOI Listing
June 2022

Effect of PPP1R14D gene high expression in lung adenocarcinoma knocked out on proliferation and apoptosis of DMS53 cell.

Clin Transl Oncol 2022 May 17. Epub 2022 May 17.

Ward 2, Department of Respiratory Medicine, The Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, 161000, China.

Purpose: Globally, lung cancer remains the most commonly diagnosed cancer and the leading cause of cancer-related mortality. Lung adenocarcinoma (LUAD) is a common subtype of lung cancer and carries a poor prognosis. Treatment outcomes biomarkers in LUAD are critical, and there is currently a paucity of data; therefore, there is a need for novel biomarkers and newer therapeutic targets.

Methods: Bayesian analysis was used to obtain the whole-genome t value of LUAD. Gene set enrichment analysis (GSEA) was conducted to obtain the normalized enrichment scores (NES) of the whole genome, and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway was analyzed using the Gene Set Analysis Toolkit. Herein, we investigated the PPP1R14D expression level at the protein level in LUAD and the impact of PPP1R14D knockdown on the proliferation and apoptosis of LUAD cells in vitro.

Results: A total of 483 LUAD samples and 59 normal control samples were included, and 904 differentially expressed genes (DEGs) and 504 LUAD-related genes reported in the literature were obtained. The DEGs showed that PPP1R14D was the most significantly up-regulated gene. Western blot of 30 cases of LUAD tissue and adjacent normal tissue also found that PPP1R14D was significantly highly expressed in cancer tissues. Lentivirus-mediated shRNA strategy effectively inhibited PPP1R14D expression in human LUAD cells DMS53, while PPP1R14D knockdown induced apoptosis and cell proliferation in DMS53 cells.

Conclusion: Abnormally up-regulated PPP1R14D promotes the survival and proliferation of tumor cells in human LUAD and may serve as a therapeutic and diagnostic target for LUAD.
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http://dx.doi.org/10.1007/s12094-022-02842-7DOI Listing
May 2022

Bioinspired Superspreading Surface: From Essential Mechanism to Application.

Acc Chem Res 2022 Jun 15;55(11):1467-1479. Epub 2022 May 15.

Key Laboratory of Bioinspired Smart Interfacial Science, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China.

ConspectusThe dynamic behavior of liquids on surfaces is ubiquitous in nature and has aroused wide attention from researchers. Among them, the superspreading surface has been extensively investigated and applied in areas ranging from film fabrication to antibiofouling, separation, etc. However, the traditional equilibrium contact angle (CA) at the thermodynamic steady-state cannot completely depict the dynamic spreading process of liquids, because the performance of these surfaces is controlled not only by the final steady superhydrophilicity (CA < 5°) but also by the superspreading speed of liquids with a CA of ∼0°. Moreover, as the most basic prerequisite for superspreading, the long-held intrinsic wetting threshold (IWT) of 90°, which divides hydrophobic and hydrophilic surfaces, is also controversial.In this Account, we summarize and condense the commonalities of our related research, further formally propose the concept of "superspreading", and recommend using "superspreading time (ST)" and "curve of superspreading radius versus spreading time (SRST)" to quantify its performance. Learning from nature is the most effective way to artificially fabricate superspreading surfaces. To begin, we first review some typical superspreading surfaces we found in nature and introduce the strategies adopted by the surfaces for surviving or realizing special functions. Then, we systematically review our recent understanding of the essential mechanism of superspreading surfaces across multiple length scales─from the molecular origin of the newly found IWT of ∼65° for water to the macroscopic respective functions of nanostructure and microstructure in superspreading. Armed with the in-depth fundamental mechanism, we propose the designing principle of high-performance superspreading surfaces. Following that, we summarize the commonly utilized methods, including modifying surface composition to give the surface intrinsic hydrophilicity and changing surface structure to improve the superspreading performance. Subsequently, we introduce the recently developed practical applications by virtue of the outstanding property of the superspreading surface, including the fabrication of a self-assembled film on the solid-gas surface and solid-liquid interface, a self-assembled water barrier for antibiofouling and oil repellency, high-efficiency separation and heat dissipation, etc. Finally, we discuss the remaining major challenges and the future development trends in the superspreading field. This Account serves to arouse wide attention and efforts in the superspreading field to strengthen mechanism research and promote practical large-area applications.
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http://dx.doi.org/10.1021/acs.accounts.2c00042DOI Listing
June 2022

Magnetic Self-Healing Hydrogel from Difunctional Polymers Prepared via the Kabachnik-Fields Reaction.

ACS Macro Lett 2022 01 16;11(1):39-45. Epub 2021 Dec 16.

The Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, People's Republic of China.

The development of high quality magnetic self-healing hydrogels containing well-dispersed magnetic nanoparticle has been a challenging procedure due to unavailable methods of facilely introducing groups that can efficiently stabilize these magnetic nanoparticles in the self-healing hydrogels. In this research, a polymer containing both phenylboronic acid (PBA) and phosphonic acid (PA) groups has been developed by the Kabachnik-Fields (KF) reaction. This polymer well disperses iron oxide nanoparticles (IONPs) through the strong interactions between the PA groups and the surface of the IONPs; thus, this polymer effectively mixed IONPs and poly(vinyl alcohol) (PVA) to form a hydrogel containing well-dispersed IONPs. The resulting hydrogel is self-healing, owing to the dynamic borate ester linkages. Moreover, the presence of the IONPs endowed the hydrogel with magnetic properties, also making it heat-responsive in an alternating magnetic field and expanding its application as a contrast agent for magnetic resonance imaging. The magnetic self-healing hydrogel showed excellent biosafety properties in animal experiments, suggesting its potential as an injectable implant material for biological and medical applications. This research exploits a biocompatible magnetic self-healing hydrogel with well-dispersed IONPs, demonstrating the value of the KF reaction in the development of functional polymers and smart materials, which might prompt a broad study of multicomponent reactions in interdisciplinary fields.
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http://dx.doi.org/10.1021/acsmacrolett.1c00720DOI Listing
January 2022

Kudzu Celery Decoction Exerts Protection against Sepsis-Induced Myocardial Injury.

Oxid Med Cell Longev 2022 4;2022:2886932. Epub 2022 May 4.

Xi'an Key Laboratory of Cardiovascular and Cerebrovascular Diseases, Xi'an No. 3 Hospital, The Affiliated Hospital of Northwest University, School of Life Sciences and Medicine, Northwest University, 10 Fengcheng Three Road, Xi'an, China.

Myocardial dysfunction is well-recognized manifestations of organ dysfunction in sepsis, which is the leading cause of death in critically ill patients. The underlying mechanisms associated with sepsis-induced myocardial injury (SIMI) include cardiac contractility, inflammatory response, oxidative stress, and apoptosis. Kudzu celery decoction (KCD) is composed of a variety of traditional Chinese medicine (TCM) such as kudzu and celery. The previous study found that the main ingredients in kudzu and celery have also been proved to have anti-inflammatory, antioxidative, and other biological activities. In this study, the therapeutic effects of KCD were evaluated in the cecal ligation and puncture (CLP) model of BALB/c mice. The effects of KCD on cardiac function, myocardium damage, inflammation, and fibrosis in CLP-injured mice were analyzed with echocardiography, histological staining, and quantitative real-time PCR. The results showed that KCD treatment improved the anal temperature, sepsis score, blood routine parameters, and blood biochemical parameters in CLP-injured mice. Then, we observed that KCD could remarkably alleviate cardiac dysfunction, myocardial fibrosis, oxidative stress, and inflammation in CLP-injured mice. In this study, we confirmed that KCD has a significant protective effect on SIMI, which may favor KCD a potential cardioprotective drug candidate to alleviate SIMI and further amplify the application of TCM prescription in clinic.
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http://dx.doi.org/10.1155/2022/2886932DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9095356PMC
May 2022
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