Publications by authors named "Ye Ma"

133 Publications

Long non-coding RNA HOTAIR/microRNA-761 sponge regulates PPME1 and further influences cell biological functions in thyroid carcinoma.

Laryngoscope Investig Otolaryngol 2021 Jun 26;6(3):438-445. Epub 2021 May 26.

Department of General Surgery Jiading District Central Hospital Affiliated to Shanghai University of Medicine & Health Sciences Shanghai China.

Background: Most well-differentiated thyroid carcinomas display good therapeutic outcomes, but there are still some patients who are not sensitive to the general treatments lose their treatment opportunities. Thus, it is important to understand the molecular mechanisms that cause thyroid carcinoma, so as to find effective diagnostic and therapeutic targets.

Aim Of The Study: To explore the role of homeobox transcript antisense RNA (HOTAIR) in thyroid carcinoma through protein phosphatase methylesterase 1 (PPME1) by sponging microRNA 761 (miR-761).

Methods: The regulation network amongst HOTAIR, miR-761 and PPME1 was predicted by online sources. RT-PCR was conducted to evaluate the expression of HOTAIR and miR-761 in tumor tissues. Clinical data was collected and analyzed by Chi-square test. Cell apoptosis and proliferation was evaluated using three types of cancer cells (HTh-7, CAL-62, BCPAP) after treated with si-HOTAIR and miR-761inhibitor. The binding site among HOTAIR, miR-761 and PPME1 was verified by dual luciferase reporter assay. PPME1 expression was measured after HOTAIR and miR-761 were suppressed by western blot. Survival time was measured in nude mice using log-rank test.

Results: HOTAIR was expressed to a significantly greater extent than miR-761 in thyroid tumor tissues ( < .001). miR-761 and PPME1 were negatively correlated (coef = -1.91,  < .001). HOTAIR competitively binds to miR-761 and miR-761 directly targets PPME1. HOTAIR was highly correlated with TNM (  = 5.797,  = .016), tumor size (  = 7.955,  = .005) and lymphatic metastasis (  = 6.0,  = .014). HOTAIR promoted cell proliferation and inhibited cell apoptosis, whereas miR-761 did not. HOTAIR elevated and miR-761 suppressed PPME1 expression. HOTAIR expression appears to affect the survival time in vivo.

Conclusion: HOTAIR regulated thyroid cancer cells by binding to miR-761 through PPME1.
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http://dx.doi.org/10.1002/lio2.593DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8223458PMC
June 2021

Hamartomatous polyposis syndrome associated malignancies: Risk, pathogenesis and endoscopic surveillance.

J Dig Dis 2021 Jun 18. Epub 2021 Jun 18.

Department of Gastroenterology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China.

Hamartomatous polyposis syndromes (HPS) are a heterogeneous spectrum of diseases that are characterized by diffuse hamartomatous polyps lining the gastrointestinal (GI) tract together with extra-GI manifestations. Classical HPS includes juvenile polyposis syndrome, Peutz-Jeghers syndrome, PTEN hamartoma tumor syndrome and hereditary mixed polyposis syndrome. Patients with HPS have a higher risk of GI and extra-GI malignancies than the general population, although the underlying mechanisms remain unclear and are obviously different from the carcinogenesis of classical adenocarcinoma and colitis-associated malignancy. In this review we aimed to clarify the risks, possible mechanism and endoscopic surveillance of HPS-associated GI malignancies.
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http://dx.doi.org/10.1111/1751-2980.13029DOI Listing
June 2021

Using photo-induced p-n junction interface effect of CoMnO/β-MnO oxidase mimetics for colorimetric determination of hydroquinone in seawater.

Anal Chim Acta 2021 Aug 26;1172:338695. Epub 2021 May 26.

Department of Materials Science and Engineering, Ocean University of China, 266100, Qingdao, PR China.

Detection of pollutants in seawater faces a great challenge of strong interference, and the facile detection method is lacked. The CoMnO/β-MnO p-n junction oxidase mimetics were successfully prepared for colorimetric detection of hydroquinone in seawater. The catalysis ability was enhanced significantly by the photo-induced p-n junction interface effect. It not only promoted the formation of HO by suppressing the recombination of photon-generated carriers, but also provided the driving force for electron transport. The colorimetric detection of hydroquinone was achieved by fading and exhibited good adaptability in seawater. The obtained good recovery rate (97.23%-101.37%) in seawater makes it an inspiring method for practical application. The photo-induced p-n junction interface effect provides an opportunity for developing the application of colorimetric sensing in seawater detection.
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http://dx.doi.org/10.1016/j.aca.2021.338695DOI Listing
August 2021

Self-assembling two-dimensional nanophotonic arrays for reflectivity-based sensing.

Chem Sci 2020 Aug 13;11(35):9563-9570. Epub 2020 Aug 13.

Department of Chemistry, Imperial College London, Molecular Science Research Hub, White City Campus 80 Wood Lane W12 0BZ UK

We propose a nanoplasmonic platform that can be used for sensing trace levels of heavy metals in solutions simple optical reflectivity measurements. The considered example is a lead sensor, which relies on the lead-mediated assembly of glutathione-functionalized gold nanoparticles (NPs) at a self-healing water/DCE liquid | liquid interface (LLI). Capillary forces tend to trap each NP at the LLI while the negatively charged ligands prevent the NPs settling too close to each other. In the presence of lead, due to chelation between the lead ion and glutathione ligand, the NPs assemble into a dense quasi-2D interfacial array. Such a dense assembly of plasmonic NPs can generate a remarkable broad-band reflectance signal, which is absent when NPs are adsorbed at the interface far apart from each other. The condensing effect of the LLI and the plasmonic coupling effect among the NP array gives rise to a dramatic enhancement of the reflectivity signals. Importantly, we show that our theory of the optical reflectivity from such an array of NPs works in perfect harmony with the physics and chemistry of the system with the key parameter being the interparticle distance at the interface. As a lead sensor, the system is fast, stable, and can achieve detection limits down to 14 ppb. Future alternative recognizing ligands can be used to build sister platforms for detecting other heavy metals.
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http://dx.doi.org/10.1039/d0sc02877kDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161679PMC
August 2020

Periodic mesoporous organosilica-coated magnetite nanoparticles combined with lipiodol for transcatheter arterial chemoembolization to inhibit the progression of liver cancer.

J Colloid Interface Sci 2021 Jun 10;591:211-220. Epub 2021 Feb 10.

Department of Interventional Therapy, Department of Radiology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing 210002, Jiangsu, PR China. Electronic address:

Transcatheter arterial chemoembolization (TACE) is standard locoregional therapy for hepatocellular carcinoma (HCC) that involves the injection of chemotherapeutic drugs with embolic agents into tumor tissues through intra-arterial transcatheter infusion. TACE technology using lipiodol emulsion has been most widely used in the treatment of human HCC. However, lipiodol emulsions with anticancer drugs do not stably maintain high drug concentrations at tumor sites. Herein, we developed a dual-modality imaging nanoplatform for the TACE treatment of liver cancer by integrating periodic mesoporous organosilica (PMO) with magnetite (FeO) nanoparticles and Cy5.5 molecules (denoted as [email protected]). [email protected] showed an excellent doxorubicin (Dox)-loading capacity, sensitive drug release behavior under acidic conditions, and good biocompatibility. Moreover, Cy5.5-mediated optical imaging showed that Dox-loaded [email protected] ([email protected]) could enter liver cancer cells and effectively inhibit their growth. In addition, [email protected] was used in combination with transarterial embolization for the treatment of in situ VX2 liver tumors in rabbits. Magnetic resonance imaging (MRI) evaluation showed that [email protected] perfused through arteries was deposited into liver tumors, and [email protected] combined with lipiodol to control liver tumors yielded the optimal therapeutic effect. In addition, histological analysis showed that compared with both lipiodol embolization and traditional lipiodol combined with Dox chemoembolization, [email protected] combined with lipiodol chemoembolization induced more complete tumor tissue necrosis. In summary, these results indicate that the [email protected] platform has the potential to become an advanced tool for the transarterial treatment of unresectable liver cancer.
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http://dx.doi.org/10.1016/j.jcis.2021.02.022DOI Listing
June 2021

Transcatheter tricuspid valve replacement in patients with severe tricuspid regurgitation.

Heart 2021 Jan 8. Epub 2021 Jan 8.

Department of Cardiovascular Surgery, Changhai Hospital, Second Military Medical University, Shanghai, China

Objective: Tricuspid regurgitation (TR) is a common valvular heart disease with unsatisfactory medical therapeutics and high surgical mortality. The present study aims to evaluate the safety and effectiveness of transcatheter tricuspid valve replacement (TTVR) in high-risk patients with severe TR.

Methods: This was a compassionate multicentre study. Between September 2018 and November 2019, 46 patients with TR who were not suitable for surgery received compassionate TTVR under general anaesthesia and the guidance of trans-oesophageal echocardiography and fluoroscopy in four institutions. Access to the tricuspid valve was obtained via a minimally invasive thoracotomy and transatrial approach. Patients' data at baseline, before discharge, 30 days and 6 months after the procedure were collected.

Results: All patients had severe TR with vena contracta width of 12.6 (11.0, 14.5) mm. Procedural success (97.8%) was achieved in all but one case with right ventricle perforation. The procedural time was 150.0 (118.8, 180.0) min. Intensive care unit time was 2.0 (1.0, 4.0) days. 6-month mortality was 17.4%. Device migration occurred in one patient (2.4%) during follow-up. Transthoracic echocardiography at 6 months after operation showed TR was significantly reduced (none/trivial in 33, mild in 4 and moderate in 1) and the primary safety end point was achieved in 38 cases (82.6%). Patients suffered from peripheral oedema and ascites decreased from 100.0% and 47.8% at baseline to 2.6% and 0.0% at 6 months.

Conclusions: The present study showed TTVR was feasible, safe and with low complication rates in patients with severe TR.
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http://dx.doi.org/10.1136/heartjnl-2020-318199DOI Listing
January 2021

Fabrication of the Ni-based composite wires for electrochemical detection of copper(Ⅱ) ions.

Anal Chim Acta 2021 Jan 24;1143:45-52. Epub 2020 Nov 24.

Department of Materials Science and Engineering, Ocean University of China, 266100, Qingdao, PR China.

Copper ions (Cu) pollution in the water environment poses a great threat to the health function of life-sustaining metabolic activities. However, the current detection methods need relatively expensive instruments, complex operation procedures and long time, so a facile and direct detection method is desired to be developed. In this work, the Ni-based composite wires with p-n junction (the Ni/NiO/ZnO/Chitosan wire) and Schottky junction (the Ni/NiO/Au/Chitosan wire) were fabricated, and the barrier driven electrochemical sensing mechanism was studied. The direct and facile detection of Cu was achieved with a wide linear range (0-6000 nM) and a low LOD (0.81 nM). The excellent stability and recovery in real water samples made the Ni-based composite wires a promising candidate for the practical application. The interfacial barriers of semiconductor can be used as a special sensing factor to develop novel sensors.
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http://dx.doi.org/10.1016/j.aca.2020.11.028DOI Listing
January 2021

miR-214 Attenuates Aortic Valve Calcification by Regulating Osteogenic Differentiation of Valvular Interstitial Cells.

Mol Ther Nucleic Acids 2020 Dec 15;22:971-980. Epub 2020 Oct 15.

Department of Cardiothoracic Surgery, Changhai Hospital, Second Military Medical University, Shanghai, 200433, China.

Calcific aortic valve disease (CAVD) is a common heart valve disease in aging populations, and aberrant osteogenic differentiation of valvular interstitial cells (VICs) plays a critical role in the pathogenesis of ectopic ossification of the aortic valve. miR-214 has been validated to be involved in the osteogenesis process. Here, we aim to investigate the role and mechanism of miR-214 in CAVD progression. miR-214 expression was significantly downregulated in CAVD aortic valve leaflets, accompanied by upregulation of osteogenic markers. Overexpression of miR-214 suppressed osteogenic differentiation of VICs, while silencing the expression of miR-214 promoted this function. miR-214 directly targeted ATF4 and Sp7 to modulate osteoblastic differentiation of VICs, which was proved by dual luciferase reporter assay and rescue experiment. miR-214 knockout rats exhibited higher mean transvalvular velocity and gradient. The expression of osteogenic markers in aortic valve leaflets of miR-214 knockout rats was upregulated compared to that of the wild-type group. Taken together, our study showed that miR-214 inhibited aortic valve calcification via regulating osteogenic differentiation of VICs by directly targeting ATF4 and Sp7, indicating that miR-214 may act as a profound candidate of targeting therapy for CAVD.
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http://dx.doi.org/10.1016/j.omtn.2020.10.016DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7679242PMC
December 2020

Cyanine-modified near-infrared upconversion nanoprobe for ratiometric sensing of NH in living cells.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Feb 4;247:119153. Epub 2020 Nov 4.

College of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, PR China. Electronic address:

Although being as an important chemical material in industry, hydrazine (NH) is highly toxic to the humans and animals. The development of sensitive methods for the detection of hydrazine is meaningful. Herein, we develop a new organic-inorganic hybrid nanoprobe for the detection of NH based on luminescent resonance energy transfer (LRET) process. The nanoprobe contains NH-responsive NIR cyanine dye (CQM1) and α-cyclodextrin (CD) anchored on the surface of lanthanide-doped upconversion nanophosphors (UCNPs). In the presence of hydrazine, the hybrid materials (CQM1-UCNPs) showed the a large ratiometric luminescent signal change with high sensitivity and selectivity. More importantly, by taking advantage of ratiometric Upconversion luminescent (UCL) signal and the features of NIR emission/excitation, the nanoprobe was successfully applied for visualization of hydrazine in living cells for the first time.
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http://dx.doi.org/10.1016/j.saa.2020.119153DOI Listing
February 2021

ALS/FTLD-Linked Mutations in FUS Glycine Residues Cause Accelerated Gelation and Reduced Interactions with Wild-Type FUS.

Mol Cell 2020 11 6;80(4):666-681.e8. Epub 2020 Nov 6.

Program in Cell, Molecular, Developmental Biology, and Biophysics, Johns Hopkins University, 3400 N Charles St, Baltimore, MD 21218, USA; Department of Physics, Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Department of Biophysics, Johns Hopkins University, 3400 N Charles St, Baltimore, MD 21218, USA. Electronic address:

The RNA-binding protein fused in sarcoma (FUS) can form pathogenic inclusions in neurodegenerative diseases like amyotrophic lateral sclerosis (ALS) and frontotemporal lobar dementia (FTLD). Over 70 mutations in Fus are linked to ALS/FTLD. In patients, all Fus mutations are heterozygous, indicating that the mutant drives disease progression despite the presence of wild-type (WT) FUS. Here, we demonstrate that ALS/FTLD-linked FUS mutations in glycine (G) strikingly drive formation of droplets that do not readily interact with WT FUS, whereas arginine (R) mutants form mixed condensates with WT FUS. Remarkably, interactions between WT and G mutants are disfavored at the earliest stages of FUS nucleation. In contrast, R mutants physically interact with the WT FUS such that WT FUS recovers the mutant defects by reducing droplet size and increasing dynamic interactions with RNA. This result suggests disparate molecular mechanisms underlying ALS/FTLD pathogenesis and differing recovery potential depending on the type of mutation.
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http://dx.doi.org/10.1016/j.molcel.2020.10.014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7688085PMC
November 2020

Immune restoration in HIV-1-infected patients after 12 years of antiretroviral therapy: a real-world observational study.

Emerg Microbes Infect 2020 Dec;9(1):2550-2561

National Center for AIDS/STD Control and Prevention, China Center for Disease Control and Prevention, Beijing, People's Republic of China.

Using normalization of CD4 counts as the main evaluation parameter of complete immune restoration for HIV-1 patients under antiretroviral therapy (ART) might be not enough. A comprehensive evaluation system more accurately reflecting immune restoration are urgently needed. Totally, 91,805 HIV-1 patients from 17 tertiary hospitals in China during 2005-2018 were included in this study. Immune restoration and mortality were assessed. Patients initiated ART with baseline CD4 counts <50, 50-199, 200-349, 350-499, and ≥500 cells/μL, and results showed an increase in the median CD4 counts to 445 (12-year), 467 (12-year), 581 (11-year), 644 (7-year), and 768 cells/µL (5-year), as well as the CD4/CD8 ratio to 0.59 (12-year), 0.65 (12-year), 0.79 (11-year), 0.82 (7-year), 0.9 (5-year), respectively. The median CD8 count was relatively high (median range 732-845 cells/μL), regardless of the baseline CD4 counts. Furthermore, the probabilities of death in patients achieving CD4 counts ≥500 cells/μL and CD4/CD8 ratio ≥0.8 simultaneously were significantly lower than those in patients achieving either CD4 counts ≥500 cells/μL (2.77% vs 3.50%, =0.02) or CD4/CD8 ≥ 0.8 (2.77% vs 4.28%, <0.001) after 12-year of ART. In this study, a new binary-indicator would accurately assess immune restoration in the era of "treat all."
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http://dx.doi.org/10.1080/22221751.2020.1840928DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7733958PMC
December 2020

Impact of prosthesis-patient mismatch on early and late outcomes after mitral valve replacement: a meta-analysis.

J Geriatr Cardiol 2020 Aug;17(8):455-475

Department of Cardiothoracic Surgery, Changhai Hospital, Naval Military Medical University (The Second Military Medical University), Shanghai, China.

Background: Prognostic significance of prosthesis-patient mismatch (PPM) after mitral valve replacement (MVR) remains uncertain because of the limited studies reporting inconsistent or even contrary results. This meta-analysis pooled results of all available studies comparing early and late prognoses between patients with significant mitral PPM and those without.

Methods: Studies were identified by searching Pubmed, Excerpta Medica Database, Cochrane Central Register of Controlled Trials, and ClinicalTrials.gov. Impact of PPM on postoperative hemodynamic results, thirty-day mortality, overall mortality, mortality of thirty-day survivors, and primary morbidity after MVR was evaluated via meta-analysis. Robustness of pooled estimates, source of heterogeneity, and publication bias were assessed via sensitivity analyses, meta-regression as well as subgroup analysis stratified according to methodological or clinical heterogeneity, or sequential omission method, and funnel plot or Begg's and Egger's tests, respectively.

Results: Nineteen cohort studies involving 9302 individuals (PPM group: = 5109, Control group: = 4193) were included for meta-analysis. Total PPM and severe PPM prevalence were 3.8%-85.9% and 1%-27%, with a mean value of 54.9% and 14.1%, respectively. As compared with control group, mitral PPM group demonstrated a poorer postoperative hemodynamic status of higher mean and peak residual transprosthetic pressure gradients (TPG), higher postoperative systolic pulmonary artery pressure (SPAP) and less reduction, higher postoperative pulmonary hypertension (PH) prevalence and less PH regression, smaller net atrioventricular compliance, less NYHA class decrease, higher postoperative functional tricuspid regurgitation prevalence and less regression. The PPM group also revealed a higher thirty-day mortality, long-term overall mortality, mortality of thirty-day survivors, and postoperative congestive heart failure prevalence, which were positively correlated with the severity of PPM if it was classified into tri-level subgroups. Left ventricular end-diastolic diameter, postoperative atrial fibrillation (AF) prevalence, and the AF regression were analogous between groups. Most pooled estimates were robust according to sensitivity analyses. Male patients and bioprosthesis implantation proportion were prominent source of between-study heterogeneity on thirty-day mortality. Publication bias was not significant in tests for all the outcomes, except for SPAP and TPG.

Conclusions: Mitral PPM would result in poorer postoperative hemodynamics and worse early and late prognosis. Severe PPM must be avoided since deleterious impact of mitral PPM was severity dependent.
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http://dx.doi.org/10.11909/j.issn.1671-5411.2020.08.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7475218PMC
August 2020

Fabrication and Microstructure of ZnO/HA Composite with In Situ Formation of Second-Phase ZnO.

Materials (Basel) 2020 Sep 7;13(18). Epub 2020 Sep 7.

School of Materials Science and Engineering, Jiamusi University, Jiamusi 154007, China.

Nanometer hydroxyapatite (n-HA) powders were synthesized by the chemical precipitation method, and a novel ZnO/HA composite, which consisted of second-phase particles with different sizes and distributions, was successfully fabricated. ZnO/HA composites were prepared by using powder sintering with different Zn contents and a prefabrication pressure of 150 MPa. Microstructure and local chemical composition were analyzed by a scanning electron microscope (SEM) and energy-dispersive spectrometer (EDS), respectively. The phase composition and distribution of the composite were determined with electron back-scattered diffraction (EBSD) and an X-ray diffractometer (XRD), respectively. The experimental results of the XRD showed that the chemical precipitation method was a simple and efficient method to obtain high-purity n-HA powders. When the sintering temperature was lower than 1250 °C, the thermal stability of HA was not affected by the Zn in the sintering process. Due to sintering in an air atmosphere, the oxidation reaction of Zn took place in three stages, and ZnO as the second phase had two different sizes and distributions in the composites. The compressive strength of ZnO/HA composites, of which the highest was up to 332 MPa when the Zn content was 20%, was significantly improved compared with pure HA. The improvement in mechanical properties was mainly due to the distribution of fine ZnO particles among HA grains, which hindered the HA grain boundary migration and refinement of HA grains. As grain refinement increased the area of the grain boundary inside the material, both the grain boundary and second phase hindered crack development in different ways.
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http://dx.doi.org/10.3390/ma13183948DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7558110PMC
September 2020

Preparation of NiMnO/C necklace-like microspheres as oxidase mimetic for colorimetric determination of ascorbic acid.

Talanta 2020 Nov 26;219:121299. Epub 2020 Jun 26.

Department of Materials Science and Engineering, Ocean University of China, 266100, Qingdao, PR China.

The NiMnO/C necklace-like microspheres (NLM) were successfully prepared by hydrothermal method and oil bath. This unique necklace-like structure makes them exhibit the enhanced intrinsic oxidase-like activity, as the special interface can help capture electrons from 3,3',5,5'-tetramethylbenzidine. The fabricated NiMnO/C NLM were successfully used as the high-performance oxidase mimetic to catalyze the oxidation of TMB directly for the color reaction. A simple colorimetric method for detection of ascorbic acid by fading was developed, and the high sensitivity with the low detection limit (0.047 μM) was achieved. It is a facile route to fabricate the NiMnO/C NLM as the high-performance oxidase mimetic for colorimetric biosensing.
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http://dx.doi.org/10.1016/j.talanta.2020.121299DOI Listing
November 2020

HMGA2 regulates circular RNA ASPH to promote tumor growth in lung adenocarcinoma.

Cell Death Dis 2020 07 27;11(7):593. Epub 2020 Jul 27.

Department of Thoracic Surgery, General Hospital of Western Theater Command, Chengdu, China.

In this study, we identified a circular form of ASPH RNA (circASPH), expression of which was upregulated in lung adenocarcinoma and the human lung adenocarcinoma cell lines. We also found a positive correlation between circASPH level and the T and N stages of lung adenocarcinoma patients. Patients with higher levels of circASPH had a shorter overall survival. Moreover, we demonstrated that circASPH was directly regulated by HMGA2 and Twist1. The direct positive regulation of circASPH by Twist1 was dependent on the presence of HMGA2. Functional assays indicated that circASPH promoted the proliferation, migration, and invasion of lung adenocarcinoma cell lines in vitro. The promoting effect of tumor growth by circASPH was also observed in vivo. Mechanistically, circASPH was identified to act as a molecular sponge for miR-370 and abrogate miR-370-mediated inhibition of HMGA2. Finally, we demonstrated that the oncogenic function of circASPH was HMGA2-dependent. These findings reveal the oncogenic functions of the HMGA2-circASPH-HMGA2 axis and may be useful in developing circRNA-based therapeutic strategies for lung adenocarcinoma.
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http://dx.doi.org/10.1038/s41419-020-2726-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7385491PMC
July 2020

Synthesis of Aluminophosphate Molecular Sieves in Alkaline Media.

Chemistry 2020 Sep 7;26(50):11408-11411. Epub 2020 Aug 7.

Key Lab of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, P. R. China.

Unlike conventional aluminosilicate zeolites synthesized in alkaline media, aluminophosphate molecular sieves (AlPOs) have always been prepared under acidic conditions in the past three decades; this has been regarded as one of essential factors for synthesis, except for the case of silica-substituted analogues (SAPOs). For the first time, we demonstrate herein a simple and generalized route for synthesizing various types of aluminophosphate molecular sieves in alkaline media. A series of aluminophosphate sieves and their analogues have been prepared with different quaternary ammonium cations as structure-directing agents in this manner. The above successes have extended the systematic media from acidic or neutral to alkaline for the preparation of a series of aluminophosphate molecular sieves, which possibly open an alternative route for the synthesis of aluminophosphate molecular sieves.
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http://dx.doi.org/10.1002/chem.202001050DOI Listing
September 2020

Reduction of NgR in perforant path decreases amyloid-β peptide production and ameliorates synaptic and cognitive deficits in APP/PS1 mice.

Alzheimers Res Ther 2020 04 24;12(1):47. Epub 2020 Apr 24.

Liaoning Provincial Key Laboratory of Cerebral Diseases, Department of Physiology, College of Basic Medical Sciences, Dalian Medical University, Dalian, China.

Background: Amyloid beta (Aβ) which is recognized as a main feature of Alzheimer's disease (AD) has been proposed to "spread" through anatomically and functionally connected brain regions. The entorhinal cortex and perforant path are the earliest affected brain regions in AD. The perforant path is the most vulnerable circuit in the cortex with respect to both aging and AD. Previous data show that the origins and terminations of the perforant path are susceptible to amyloid deposition at the younger age in AD. Nogo receptor (NgR) plays an essential role in limiting injury-induced axonal growth and experience-dependent plasticity in the adult brain. It has been suggested that NgR is involved in AD pathological features, but the results have been conflicting and the detailed mechanism needs further investigation. In this study, the effect of NgR in the perforant path on the pathological and functional phenotype of APP/PS1 transgenic mice was studied.

Methods: To genetically manipulate NgR expression, adeno-associated virus (AAV) with short hairpin (shRNA) against NgR was injected into the perforant path of APP/PS1 transgenic mice, followed by an assessment of behavioral, synaptic plasticity and neuropathological phenotypes. NgR was overexpressed or knockdown in neuroblastoma N2a cells and APPswe/HEK293 cells to investigate the interaction between NgR and amyloid precursor protein (APP).

Results: It is shown that reduction of NgR in the perforant path rescued cognitive and synaptic deficits in APP/PS1 transgenic mice. Concurrently, Aβ production in the perforant path and levels of soluble Aβ and amyloid plaques in the hippocampus were significantly decreased. There was a positive correlation between the total APP protein level and NgR expression both in transgenic mice and in cultured cells, where the α-secretase and β-secretase cleavage products both changed with APP level in parallel. Finally, NgR might inhibit APP degradation through lysosome by Rho/Rho-associated protein kinases (ROCK) signaling pathway.

Conclusions: Our findings demonstrate that perforant path NgR plays an important role in regulating APP/Aβ level and cognitive functions in AD transgenic mice, which might be related to the suppression of APP degradation by NgR. Our study suggests that NgR in the perforant path could be a potential target for modulating AD progression.
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http://dx.doi.org/10.1186/s13195-020-00616-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7181577PMC
April 2020

Deep Learning-Based Upper Limb Functional Assessment Using a Single Kinect v2 Sensor.

Sensors (Basel) 2020 Mar 30;20(7). Epub 2020 Mar 30.

Faculty of Sports Science, Ningbo University, Ningbo 315000, China.

We develop a deep learning refined kinematic model for accurately assessing upper limb joint angles using a single Kinect v2 sensor. We train a long short-term memory recurrent neural network using a supervised machine learning architecture to compensate for the systematic error of the Kinect kinematic model, taking a marker-based three-dimensional motion capture system (3DMC) as the golden standard. A series of upper limb functional task experiments were conducted, namely hand to the contralateral shoulder, hand to mouth or drinking, combing hair, and hand to back pocket. Our deep learning-based model significantly improves the performance of a single Kinect v2 sensor for all investigated upper limb joint angles across all functional tasks. Using a single Kinect v2 sensor, our deep learning-based model could measure shoulder and elbow flexion/extension waveforms with mean CMCs >0.93 for all tasks, shoulder adduction/abduction, and internal/external rotation waveforms with mean CMCs >0.8 for most of the tasks. The mean deviations of angles at the point of target achieved and range of motion are under 5° for all investigated joint angles during all functional tasks. Compared with the 3DMC, our presented system is easier to operate and needs less laboratory space.
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http://dx.doi.org/10.3390/s20071903DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7180801PMC
March 2020

Surgical Outcomes of Stent-Related Type A Dissection Compared with Spontaneous Type A Dissection.

Ann Thorac Cardiovasc Surg 2020 Oct 10;26(5):270-275. Epub 2020 Mar 10.

Department of Cardiovascular Surgery, Changhai Hospital, Second Military Medical University, Shanghai, China.

Objective: Aortic endovascular stent implantation includes thoracic endovascular aortic repair (TEVAR), hybrid aortic repair (HAR), and ascending aorta stent implantation (AASI). In this study, we compared the surgical outcomes of stent-related type A dissection (SRTAD) compared with spontaneous type A dissection (STAD).

Methods: From July 2011 to July 2014, we identified 17 SRTAD patients received surgical repair in our institution. Propensity score-matching was used to identify 34 STAD patients as controls.

Results: Preoperative data of SRTAD group and STAD group had no statistical difference. Selective cerebral perfusion (SCP) time was longer in SRTAD group than in STAD group (P <0.05). SRTAD group had a longer cross-clamp time compared with STAD group (P <0.05). No intraoperative deaths in two groups. No differences in CPB time and concomitant procedures between two groups. In-hospital mortality was 11.76% (2 of 17) in SRTAD group and 2.9% (1 of 34) in STAD group (P <0.05). No differences were found in intensive care unit (ICU) time, ventilation, paraparesis, and other postoperative complications between SRTAD and STAD groups. No difference was found in survival rate between SRTAD and STAD groups in the postoperative 1-year follow-up.

Conclusions: SRTAD patients received surgical repair had a higher in-hospital mortality compared with STAD, but no differences were found in postoperative complications and mid-term outcomes.
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http://dx.doi.org/10.5761/atcs.oa.19-00216DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7641883PMC
October 2020

The Influence of Gender, Age, and Body Mass Index on Arch Height and Arch Stiffness.

J Foot Ankle Surg 2020 Mar - Apr;59(2):298-302

Lecturer, Research Academy of Grand Health, Faculty of Sport Science, Ningbo University, Zhejiang, China.

Arch structure and arch function play an important role in maintaining balance, bearing body weight, and absorbing ground reaction forces. However, arch structure and arch function are known to vary extensively and may be affected by factors such as gender, age, and obesity. Therefore, the purpose of this study was to examine the influence of gender, age, and body mass index (BMI) on arch height and arch stiffness. A total of 173 participants (aged 57.60 ± 11.19 years, mean BMI 25.12 ± 3.93 kg/m) participated in this cross-sectional study. A 3-dimensional laser scanner was used to measure foot structure information in each subject, from which arch height and arch stiffness were calculated. The results showed that women had low-arched feet compared with men (p = .001), and no arch stiffness difference was found. Older individuals tended to have a stiffer arch than middle-aged and younger individuals (p < .05), and no arch height difference was found. BMI had an impact on arch height (p < .05) but not arch stiffness. Finally, a weak positive relationship existed between arch height and arch stiffness (r = 0.32, p < .01). The findings suggest that gender, age, and obesity have a certain impact on arch structure and arch stiffness. Figuring out the relationship between these factors and arch structure may be helpful in understanding the bases of foot deformity and foot dysfunction.
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http://dx.doi.org/10.1053/j.jfas.2019.08.022DOI Listing
January 2021

Effects of Chronic Ephedrine Toxicity on Functional Connections, Cell Apoptosis, and CREB-Related Proteins in the Prefrontal Cortex of Rhesus Monkeys.

Neurotox Res 2020 Mar 20;37(3):602-615. Epub 2019 Dec 20.

Shantou University Medical College, No. 22 Xinling Road, Shantou, Guangdong, 515041, People's Republic of China.

Ephedrine abuse has spread in many parts of the world, severely threatening human health. The mechanism of ephedrine toxicity is still unclear. To explore the possible neural mechanisms of ephedrine toxicity, this study established a non-human primate model of ephedrine exposure, analyzed the functional connectivity changes in its prefrontal cortex through resting state BOLD-fMRI, and then inspected the pathophysiological changes as well as the expression of the cyclic adenosine monophosphate response element-binding protein (CREB), phosphorylated CREB (P-CREB), and CREB target proteins (c-fos and fosB) in the prefrontal cortex. After ephedrine toxicity, we found that the prefrontal cortex of monkeys strengthened its functional connectivity with the brain regions that perform motivation, drive, reward, and learning and memory functions and weakened its functional connectivity with the brain regions that perform cognitive control. These results suggest that ephedrine toxicity causes abnormal neural circuits that lead to the amplification and enhancement of drug-related cues and the weakening and damage of cognitive control function. Histology showed that the neurocytotoxicity of ephedrine can cause neuronal degeneration and apoptosis. Real-time PCR and Western blot showed increased expression of CREB mRNA and CREB/P-CREB/c-fos/fosB protein in the prefrontal cortex after ephedrine toxicity. Collectively, the present study indicates that the enhancement of drug-related cues and the weakening of cognitive control caused by abnormal neural circuits after drug exposure may be a major mechanism of brain function changes caused by ephedrine. These histological and molecular changes may be the pathophysiological basis of brain function changes caused by ephedrine.
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http://dx.doi.org/10.1007/s12640-019-00146-3DOI Listing
March 2020

Comparative evaluation of the physicochemical properties of nano-hydroxyapatite/collagen and natural bone ceramic/collagen scaffolds and their osteogenesis-promoting effect on MC3T3-E1 cells.

Regen Biomater 2019 Dec 5;6(6):361-371. Epub 2019 Oct 5.

National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.

The use of various types of calcium phosphate has been reported in the preparation of repairing materials for bone defects. However, the physicochemical and biological properties among them might be vastly different. In this study, we prepared two types of calcium phosphates, nano-hydroxyapatite (nHA) and natural bone ceramic (NBC), into 3D scaffolds by mixing with type I collagen (CoL), resulting in the nHA/CoL and NBC/CoL scaffolds. We then evaluated and compared the physicochemical and biological properties of these two calcium phosphates and their composite scaffold with CoL. Scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD) and compressive tests were used to, respectively, characterize the morphology, composition, distribution and the effect of nHA and NBC to collagen. Next, we examined the biological properties of the scaffolds using cytotoxicity testing, flow cytometry, immunofluorescence staining, biocompatibility testing, CCK-8 assays and RT-PCR. The results reflected that the Ca released from nHA and NBC could bind chemically with collagen and affect its physicochemical properties, including the infrared absorption spectrum and compression modulus, among others. Furthermore, the two kinds of scaffolds could promote the expression of osteo-relative genes, but showed different gene induction properties. In short, NBC/CoL could promote the expression of early osteogenic genes, while nHA/CoL could upregulate late osteogenic genes. Conclusively, these two composite scaffolds could provide MC3T3-E1 cells with a biomimetic surface for adhesion, proliferation and the formation of mineralized extracellular matrices. Moreover, nHA/CoL and NBC/CoL had different effects on the period and extent of MC3T3-E1 cell mineralization.
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http://dx.doi.org/10.1093/rb/rbz026DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6897342PMC
December 2019

Electrotunable Nanoplasmonics for Amplified Surface Enhanced Raman Spectroscopy.

ACS Nano 2020 Jan 16;14(1):328-336. Epub 2019 Dec 16.

Department of Chemistry , Imperial College London , Molecular Sciences Research Hub, White City Campus , London W12 0BZ , U.K.

Tuning the properties of optical metamaterials in real time is one of the grand challenges of photonics. Being able to do so will enable a class of adaptive photonic materials for use in applications such as surface enhanced Raman spectroscopy and reflectors/absorbers. One strategy to achieving this goal is based on the electrovariable self-assembly and disassembly of two-dimensional nanoparticle arrays at a metal | liquid interface. As expected, the structure results in plasmonic coupling between NPs in the array but perhaps as importantly between the array and the metal surface. In such a system, the density of the nanoparticle array can be reversibly controlled by the variation of electrode potential. Theory suggests that due to a collective plasmon-coupling effect  less than 1 V variation of electrode potential can give rise to a dramatic simultaneous change in optical reflectivity from ∼93% to ∼1% and the amplification of the SERS signal by up to 5 orders of magnitude. This is experimentally demonstrated using a platform based on the voltage-controlled assembly of 40 nm Au-nanoparticle arrays at a TiN/Ag electrode in contact with an aqueous electrolyte. We show that all the physics underpinning the behavior of this platform works precisely as suggested by the proposed theory, setting the electrochemical nanoplasmonics as a promising direction in photonics research.
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http://dx.doi.org/10.1021/acsnano.9b05257DOI Listing
January 2020

Gait Characteristics of Children with Spastic Cerebral Palsy during Inclined Treadmill Walking under a Virtual Reality Environment.

Appl Bionics Biomech 2019 19;2019:8049156. Epub 2019 Aug 19.

Department of Exercise Sciences, The University of Auckland, New Zealand.

Objective: To investigate gait characteristics in children with spastic cerebral palsy during inclined treadmill walking under a virtual reality environment.

Methods: Ten (CP) children and ten (TD) children were asked to walk at their comfortable speed on a treadmill at a ground level and 10° inclined. Three-dimensional kinematic data and ground reaction force data were captured in a computer-assisted rehabilitation environment system. Kinetic parameters and dynamic balance parameters were calculated using a standard biomechanical approach.

Results: During uphill walking, both groups decreased walking speed and stride length and increased peak pelvis tilt, ankle dorsiflexion, and hip flexion. Compared with TD children, CP children had decreased walking speed and stride length, decreased peak hip abduction moment, increased stance phase percentage, increased peak ankle dorsiflexion and knee flexion, and increased peak hip extension moment. The peak trunk rotation angle, ankle angle at initial contact, and stride length showed a significant group∗walking condition interaction effect.

Conclusions: CP children showed similar adjustments for most gait parameters during uphill walking as TD children. With a lower walking speed, CP children could maintain similar dynamic balance as TD children. Uphill walking magnifies the existing abnormal gait patterns of the cerebral palsy children. We suggest that during a treadmill training with an inclination, the walking speed should be carefully controlled in the case of improving peak joint loading too much.
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http://dx.doi.org/10.1155/2019/8049156DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6721380PMC
August 2019

Expression of NIS, VEGF-A and Thyroid Autoantibody in Papillary Thyroid Carcinoma with or without Hashimoto's Disease.

ORL J Otorhinolaryngol Relat Spec 2019;81(5-6):281-286. Epub 2019 Sep 3.

Department of Surgery, Jiading District Central Hospital, Shanghai University of Medicine and Health Sciences, Shanghai, China.

Background: Since the incidence of papillary thyroid carcinoma (PTC) combined with Hashimoto's thyroiditis (HT) has increased year by year, but the mechanism is still unknown, the goal of this study was to investigate the expression of Na+/I- symporter (NIS), vascular endothelial growth factor (VEGF)-A and thyroid autoantibodies in HT combined with PTC, and to explore the relationship between HT and PTC.

Methods: Real-time fluorescence quantitative polymerase chain reaction was used to detect the expression of VEGF-A and NIS in 38 cases of PTC and HT combined with PTC and in 20 cases of normal tissues; preoperative serum TPOAb and TgAb levels in each group were detected by ELISA.

Results: The expression of VEGF-A in PTC and HT combined with PTC was significantly higher than that in normal tissues (p < 0.05), and the expression of VEGF-A in HT combined with PTC was higher than that in PTC (p < 0.05). The expression of NIS mRNA in PTC and HT combined with PTC was significantly decreased compared to that in normal tissues (p < 0.05), and NIS mRNA in HT combined with PTC tissue was lower than that in PTC tissue (p < 0.05). The preoperative levels of TPOAb and TgAb in the HT combined with PTC group were higher than those in the normal group and the PTC group (p < 0.05).

Conclusion: The presence of HT may inhibit iodine uptake and cause a decrease in NIS. Increased levels of TPOAb and TgAb in HT patients lead to a high expression of TSH and stimulate VEGF secretion. This may explain why HT could accelerate the occurrence and development of PTC.
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http://dx.doi.org/10.1159/000501620DOI Listing
July 2020

Behavioral Changes and Neuronal Damage in Rhesus Monkeys after 10 Weeks of Ketamine Administration Involve Prefrontal Cortex Dopamine D2 Receptor and Dopamine Transporter.

Neuroscience 2019 09 19;415:97-106. Epub 2019 Jul 19.

Department of Radiology, The First Affiliated Hospital of Shantou University Medical College, No. 57 Changping Road, Shantou 515041, Guangdong, China; Shantou University Medical College, No. 22 Xinling Road, Shantou, Guangdong 515041, China; Guangdong Key Laboratory of Medical Molecular Imaging, No. 57 Changping Road, Shantou, Guangdong 515041, China. Electronic address:

The dopamine D2 receptor (DRD2) and dopamine transporter (DAT) play a regulatory role in dopaminergic neurotransmission and thus play an important role in drug addiction. The prefrontal cortex (PFC), a critical part of the mesencephalic dopaminergic system, is thought to be involved in the development and maintenance of drug addiction. The addiction to ketamine is thought to induce behavioral effects primarily through actions on the central nervous system. However, the neural mechanism underlying the effects of ketamine addiction remains unclear. In this study, we investigate the involvement of PFC DRD2 and DAT in ketamine addiction effects after ketamine administration for 10 weeks in nonhuman primates. To this end, after administering ketamine to rhesus monkeys for 10 weeks, we assessed changes in body weight and behavior. Additionally, neuronal changes in the PFC were examined by hematoxylin and eosin (HE) staining; the DRD2 and DAT mRNA and protein expression levels in the PFC were determined by real-time PCR and Western blot analysis, respectively. After 10-week ketamine administration, the assessment of the manifestations of toxicity in rhesus monkeys revealed significant changes in body weight and behavior, decreased DRD2 and DAT mRNA and protein expression in the PFC, and histological abnormalities including neuronal eosinophilia, pyknosis and disorderly arrangement of neurons in the PFC. These results suggest that the reduced expression of DRD2 and DAT in PFC could be involved in the behavioral and the neurological changes induced by ketamine administration, which may play an important role in the molecular mechanisms of ketamine addiction.
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http://dx.doi.org/10.1016/j.neuroscience.2019.07.022DOI Listing
September 2019

Fight against background noise in stimulated emission depletion nanoscopy.

Authors:
Ye Ma Taekjip Ha

Phys Biol 2019 07 5;16(5):051002. Epub 2019 Jul 5.

Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, United States of America.

STimulated emission depletion (STED) nanoscopy has been proposed to extend greatly our capability of using light to study a variety of biological problems with nanometer-scale resolution. However, in practice the unwanted background noise degrades the STED image quality and precludes quantitative analysis. Here, we discuss the underlying sources of the background noise in STED images, and review current approaches to alleviate this problem, such as time-gating, anti-Stokes excitation removal, and off-focus incomplete depletion suppression. Progress in correcting uncorrelated background photons in fluorescence correlation spectroscopy combined with STED (STED-FCS) will also be discussed.
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http://dx.doi.org/10.1088/1478-3975/ab255cDOI Listing
July 2019

Auxetic Thermoresponsive Nanoplasmonic Optical Switch.

ACS Appl Mater Interfaces 2019 Jun 11;11(25):22754-22760. Epub 2019 Jun 11.

Department of Chemistry , Imperial College London , London SW7 2AZ , United Kingdom.

Development and use of metamaterials have been gaining prominence in large part due to the possibility of creating platforms with "disruptive" and unique optical properties. However, to date, the majority of such systems produced using micro or nanotechnology are static and can only perform certain target functions. Next-generation multifunctional smart optical metamaterials are expected to have tunable elements with the possibility of controlling the optical properties in real time via variation in parameters such as pressure, mechanical stress, and voltage or through nonlinear optical effects. Here, we address this challenge by developing a thermally controlled optical switch, based on the self-assembly of poly( N-isopropylacrylamide)-functionalized gold nanoparticles on a planar macroscale gold substrate. We show that such meta-surfaces can be tuned to exhibit substantial changes in the optical properties in terms of both wavelength and intensity, through the temperature-controlled variation of the interparticle distance within the nanoparticle monolayer as well as its separation from the substrate. This change is based on temperature-induced auxetic expansion and contraction of the functional ligands. Such a system has potential for numerous applications, ranging from thermal sensors to regulated light harnessing.
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http://dx.doi.org/10.1021/acsami.9b05530DOI Listing
June 2019
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