Publications by authors named "Ye Lu"

292 Publications

Effects and mechanism of Chinese medicine Jiawei Yupingfeng in a mouse model of allergic rhinitis.

J Integr Med 2021 Jan 21. Epub 2021 Jan 21.

Department of Otolaryngology, Wangjing Hospital, China Academy of Chinese Medical Sciences, Beijing 100102, China.

Objective: Chinese medicine has the potential to modulate allergic rhinitis (AR). There have been studies investigating the treatment efficacy of Yupingfeng San, alone or in combination with other ingredients, in AR, though few have studied the potential mechanisms of these drugs. In the present study, we measured the effects of Jiawei Yupingfeng (JWYPF), a traditional Chinese medicine formula, on mice with ovalbumin-induced AR and explored its underlying mechanism of action.

Methods: Forty BALB/c mice were randomly divided into normal control, allergy control and two treatment groups of ten mice each. In the normal control group, mice were sensitized and challenged with saline. The mice in the allergy control and treatment groups were sensitized and challenged with ovalbumin and aluminum hydroxide gel. The treatments of JWYPF and Nasonex were administered intranasally in the AR mice for one week. Several signs of allergic inflammation, such as nasal eosinophils and inflammatory cytokines, were measured to determine the underlying mechanisms.

Results: Mice in the JWYPF and Nasonex groups had significantly lower AR symptom scores than those in the allergy control group (the mean differences between JWYPF and the allergy control, and Nasonex and the allergy control were -2.00 ± 0.35 and -2.40 ± 0.32). After treatment with JWYPF and Nasonex, the levels of ovalbumin-specific IgE and histamine were significantly reduced, as were the levels of interlukin-4 and transforming growth factor-β, while interferon-γ levels were increased (all P < 0.0001, vs. allergy control). These two treatments also significantly inhibited eosinophil and mast cell infiltration into the nasal cavity but were not statistically different from one-another.

Conclusion: JWYPF has a potential therapeutic effect on AR via adjusting the rebalance of T helper 1 and T helper 2.
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http://dx.doi.org/10.1016/j.joim.2021.01.012DOI Listing
January 2021

The effect of cognition and affect on preventive behaviors during the COVID-19 pandemic: a cross-sectional study in China.

BMC Public Health 2021 Apr 14;21(1):722. Epub 2021 Apr 14.

School of Journalism, Fudan University, Shanghai, 200433, China.

Background: The global outbreak of COVID-19 has become an international public health crisis. Specific antiviral treatments for COVID-19 are not yet available, and prevention is of particular importance to fight the virus. This study tends to explore and compare the roles of cognitive and affective factors in predicting preventive behavior adoption during the COVID-19 pandemic in China.

Methods: An online survey using a quota sampling method to collect responses from 3000 Chinese adults was conducted from March 2, 2020 to March 23, 2020. Questions included sociodemographic features, coronavirus knowledge, negative emotion, risk perception, and behavioral responses. Multiple regression analyses were conducted to examine the predictors of behavioral responses toward COVID-19.

Results: On average, respondents had low levels of knowledge about COVID-19 (the overall correct response rate was 7.5%). Most respondents reported moderate to strong negative emotions towards the virus (3.47 out of 5). The average reported perceived chance of infection was 23.89%. For behavioral responses, respondents reported low frequencies of going out for activities (1.98 out of 4) and high frequencies of taking preventive measures (3.22 out of 4). Behavioral responses toward COVID-19 were found to be determined by cognitive and affective variables. Knowledge was negatively related to frequency of going out for activities (β = - 0.11, p < .001). Negative emotion (β = 0.34, p < .001), and risk perception (β = 0.05, p = .007) were positively associated with going out for activities. The explanatory power of affective variables (ΔR = 12.1%) was greater than cognitive variables (ΔR = 1.0%). For preventive behaviors, knowledge was positively associated with preventive behaviors (β = 0.22, p < .001). Negative emotion (β = - 0.28, p < .001) and risk perception (β = - 0.05, p = .002) were all negatively associated with preventive measures. Affective variables still showed stronger explanatory power (ΔR = 8%) than cognitive variables (ΔR = 4.4%) in predicting preventive behaviors.

Conclusions: After the rising period of the COVID-19 outbreak in mainland China, cognitive and affective variables still played important roles in predicting behavioral responses. Compared with cognitive factors, affective factors demonstrated stronger explanatory power in predicting behavioral responses toward COVID-19. The findings may have implications for enhancing individual compliance with guidelines of adopting preventive behaviors in response to COVID-19.
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http://dx.doi.org/10.1186/s12889-021-10784-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8045573PMC
April 2021

hsa_circ_0001610 knockdown modulates miR-646-STAT3 axis to suppress endometrial carcinoma progression.

J Gene Med 2021 Apr 6:e3337. Epub 2021 Apr 6.

Department of Neurosurgery, The Affiliated Huai'an Hospital of Xuzhou Medical University, The Second People's Hospital of Huai'an, Huai'an, China.

Background: Endometrial carcinoma (EC) development is associated with dysregulated circular RNA profiles. The purpose of the current research is to study the role and mechanism of hsa_circ_0001610 (circ_0001610) in EC progression.

Methods: circ_0001610, microRNA (miR)-646, and signal transducer and activator of transcription 3 (STAT3) expression levels were measured in EC. Functional analyses were performed using Cell Counting Kit-8, colony formation, transwell, wound healing, flow cytometry, glycolysis, and xenograft analyses. Binding association was evaluated with dual-luciferase reporter assay.

Results: circ_0001610 levels were upregulated in EC samples (n = 30) and cells. circ_0001610 interference restrained cell proliferation, migration, and invasion, and promoted apoptosis. circ_0001610 downregulation constrained glycolysis through reducing glucose consumption, lactate production, and levels of adenosine triphosphate, extracellular acidification, hexokinase 2, and lactate dehydrogenase A, and increasing oxygen consumption rate. miR-646 is targeted by circ_0001610, and miR-646 inhibition attenuated interference of circ_0001610-mediated suppression of EC development. STAT3 was modulated by miR-646, and miR-646 upregulation restrained EC progression by decreasing STAT3. circ_0001610 silencing reduced STAT3 levels by sponging miR-646 and reduced the growth of xenograft tumor established by EC cells.

Conclusion: circ_0001610 knockdown represses EC progression through modulating the miR-646-STAT3 axis.
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http://dx.doi.org/10.1002/jgm.3337DOI Listing
April 2021

[Characteristics and Source Apportionment of Volatile Organic Compounds (VOCs) in the Automobile Industrial Park of Shanghai].

Huan Jing Ke Xue 2021 Feb;42(2):624-633

Jiading District Environmental Monitoring Station, Shanghai 201822, China.

Volatile organic compounds (VOCs) are important precursors of ozone and fine particulate matter, and have attracted more and more research attention. There are few long-term observational studies of VOCs in automobile industry parks. From January 1 to December 31,2019, 79 kinds of VOCs were quantitatively detected by on-line gas chromatograph in an automobile industrial park in Shanghai. The composition, seasonal variation, and daily variation of VOCs were analyzed. The chemical reactivity of the atmosphere was estimated using the maximum incremental reactivity (MIR) and·OH radical loss rate. The sources of VOCs were analyzed using specific pollutant ratios and factor analysis. The results showed that the total VOCs concentration was 26.53×10, with alkanes, alkenes, aromatics, halo hydrocarbon, and alkynes accounting for 50.2%, 9.8%, 22.4%, 10.8%, and 6.8%, respectively. There was an obviously seasonal variation in VOCs concentrations, with the maximum occurring in winter and the minimum in summer. Ozone formation potential (OFP) was 73.2×10, of which alkanes accounted for 14.7%, alkenes 35.9%, and aromatics 45.2%. The·OH radical loss rate was 165.3 s, of which alkenes accounted for 30.4% and aromatics 48.9%. The components with the highest contributions to chemical reaction activity were -xylene, ethylene, propylene, toluene, and -xylene. By estimating toluene/benzene ratios (T/B) and ethane/acetylene ratios (E/E), the air mass at the observation site was fresh, site was close to the pollution source. The main sources of VOCs were gasoline exhaust emissions (19.4%), solvent use (30.8%), combustion processes (11.0%), diesel use (8.9%), and liquefied petroleum gas use (4.5%).
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http://dx.doi.org/10.13227/j.hjkx.202005207DOI Listing
February 2021

Synthesis and antimicrobial activity evaluation of imidazole-fused imidazo[2,1-b][1,3,4]thiadiazole analogues.

ChemMedChem 2021 Mar 18. Epub 2021 Mar 18.

Yanbian University, College of Pharmacy, Gongyuan, 133002, Yanji, CHINA.

Three series of new imidazole-fused imidazo[2,1-b][1,3,4]thiadiazole analogues (compounds 20a-g, 21a-g, and 22a-g) were synthesized and their antibacterial and antifungal activities were evaluated. All the target compounds showed strong antifungal activity and high selectivity for the test fungus Candida albicans (C. albicans) over Gram-positive and Gram-negative bacteria. N-((4-(2-Cyclopropyl-6-(4-fluorophenyl)imidazo[2,1-b][1,3,4]thiadiazol-5-yl)-5-(6-methyl-pyridin-2-yl)-1H-imidazol-2-yl)methyl)aniline (21a) showed the highest activity against C. albicans (MIC50 = 0.16 µg/mL), 13-fold and 3-fold that of the positive control compounds gatifloxacin and fluconazole, respectively. Compounds 21a and 20e did not show cytotoxicity against human foreskin fibroblast-1 cells, and compound 21a was as safe as the positive control compounds in hemolysis tests. These results strongly suggest that some of the compounds produced in this work have value for development as antifungal agents.
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http://dx.doi.org/10.1002/cmdc.202100122DOI Listing
March 2021

CIPK11: a calcineurin B-like protein-interacting protein kinase from Nitraria tangutorum, confers tolerance to salt and drought in Arabidopsis.

BMC Plant Biol 2021 Mar 1;21(1):123. Epub 2021 Mar 1.

Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, 210037, China.

Background: The CIPKs are a group of plant-specific Ser/Thr protein kinases acting in response to calcium signaling, which plays an important role in the physiological and developmental adaptation of plants to adverse environments. However, the functions of halophyte-derived CIPKs are still poorly understood, that limits a potential application of CIPKs from halophytes for improving the tolerance of glycophytes to abiotic stresses.

Results: In this study, we characterized the NtCIPK11 gene from the halophyte Nitraria tangutorum and subsequently analyzed its role in salt and drought stress tolerance, using Arabidopsis as a transgenic model system. NtCIPK11 expression was upregulated in N. tangutorum root, stem and blade tissues after salt or drought treatment. Overexpressing NtCIPK11 in Arabidopsis improved seed germination on medium containing different levels of NaCl. Moreover, the transgenic plants grew more vigorously under salt stress and developed longer roots under salt or drought conditions than the WT plants. Furthermore, NtCIPK11 overexpression altered the transcription of genes encoding key enzymes involved in proline metabolism in Arabidopsis exposed to salinity, however, which genes showed a relatively weak expression in the transgenic Arabidopsis undergoing mannitol treatment, a situation that mimics drought stress. Besides, the proline significantly accumulated in NtCIPK11-overexpressing plants compared with WT under NaCl treatment, but that was not observed in the transgenic plants under drought stress caused by mannitol application.

Conclusions: We conclude that NtCIPK11 promotes plant growth and mitigates damage associated with salt stress by regulating the expression of genes controlling proline accumulation. These results extend our understanding on the function of halophyte-derived CIPK genes and suggest that NtCIPK11 can serve as a candidate gene for improving the salt and drought tolerance of glycophytes through genetic engineering.
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http://dx.doi.org/10.1186/s12870-021-02878-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7919098PMC
March 2021

Genome-wide scan of long noncoding RNA single nucleotide polymorphisms and pancreatic cancer susceptibility.

Int J Cancer 2021 Jun 3;148(11):2779-2788. Epub 2021 Feb 3.

Department of Biology, University of Pisa, Pisa, Italy.

Pancreatic ductal adenocarcinoma (PDAC) is projected to become the second cancer-related cause of death by 2030. Identifying novel risk factors, including genetic risk loci, could be instrumental in risk stratification and implementation of prevention strategies. Long noncoding RNAs (lncRNAs) are involved in regulation of key biological processes, and the possible role of their genetic variability has been unexplored so far. Combining genome wide association studies and functional data, we investigated the genetic variability in all lncRNAs. We analyzed 9893 PDAC cases and 9969 controls and identified a genome-wide significant association between the rs7046076 SNP and risk of developing PDAC (P = 9.73 × 10 ). This SNP is located in the NONHSAG053086.2 (lnc-SMC2-1) gene and the risk allele is predicted to disrupt the binding of the lncRNA with the micro-RNA (miRNA) hsa-mir-1256 that regulates several genes involved in cell cycle, such as CDKN2B. The CDKN2B region is pleiotropic and its genetic variants have been associated with several human diseases, possibly though an imperfect interaction between lncRNA and miRNA. We present a novel PDAC risk locus, supported by a genome-wide statistical significance and a plausible biological mechanism.
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http://dx.doi.org/10.1002/ijc.33475DOI Listing
June 2021

Efficacy of topical administration for oral lichen planus: A network meta-analysis.

Oral Dis 2021 Feb 2. Epub 2021 Feb 2.

State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Oral Medicine, West China Hospital of Stomatology, Sichuan University, Chengdu, China.

Objective: To examine the comparative efficacy and safety of topical administration for oral lichen planus.

Materials And Methods: An electronic database search (1st January 1946 to 1st May 2020) for randomised controlled trials identified 34 studies involving eight interventions (clobetasol, betamethasone, triamcinolone, dexamethasone, fluocinolone, tacrolimus, pimecrolimus, and cyclosporine); these studies were subjected to network meta-analysis using direct and indirect comparisons [efficacy indicators: clinical response rate, symptom-reducing effect (visual analogue scale score), sign-reducing effect (Thongprasom-scale score) and relapse; safety indicator: adverse event occurrence].

Results: Compared with placebo, tacrolimus had the best clinical response rate (odds ratio (OR), 57.78 [95% CI 3.15-1060.52]; P-score, 0.8654) and cyclosporine had the worst (OR, 3.61[95% CI 0.20-66.62]; P-score, 0.2236); tacrolimus had the best symptom-reducing effect (standardised mean difference (SMD), 1.06 [95% CI 0.41-1.71]; P-score, 0.9323) and fluocinolone had the worst (SMD, -0.54 [95% CI -1.44-0.36]; P-score, 0.0157); dexamethasone had the best sign-reducing effect (SMD, 3.60 [95% CI 1.74-5.45]; P-score, 0.8306) and clobetasol had the worst (SMD, 2.63 [95% CI 1.66-3.61]; P-score, 0.2581); and pimecrolimus performed best (OR, 0.04 [95% CI 0.00-0.64]; P-score, 0.9227) and clobetasol performed the worst [OR, 0.60; 95% CI 0.15-2.45; P-score, 0.2545] in reducing relapse. Regarding safety, dexamethasone was the safest compared with placebo [OR, 0.37; 95% CI 0.05-2.57; P-score, 0.9337), whereas fluocinolone ranked low for safety [OR, 9.48; 95% CI 1.50- 60.03; P-score, 0.1189].

Conclusions: The relative ranking of topical administration varies according to the different indicators. Based on the joint consideration of clinical response rate and adverse event occurrence, dexamethasone, triamcinolone and betamethasone are recommended for better efficacy and safety. The optimal treatment for oral lichen patients varies under different conditions.
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http://dx.doi.org/10.1111/odi.13790DOI Listing
February 2021

MicroRNA miR-330-3p suppresses the progression of ovarian cancer by targeting RIPK4.

Bioengineered 2021 Dec;12(1):440-449

Department of Pathology, Jiangxi Maternal and Child Health Hospital , Nanchang, Jiangxi, China.

Previous studies reported that miR-330-3p was involved in the progression of several cancers, but the potential roles of miR-330-3p in ovarian cancer (OC) were unclear. In the current study, we aimed to explore the expression pattern and functions of miR-330-3p in OC. The expression level of miR-330-3p in OC tissues and cell lines was detected using RT-qPCR. The proliferation, migration and invasion of OC cells were detected using CCK-8 assay and transwell assay, respectively. Bioinformatics analysis and luciferase reporter assay were used to analyze the targeted binding site of miR-330-3p and RIPK4. The results showed that miR-330-3p was significantly downregulated in OC tissues and cell lines. Overexpression of miR-330-3p inhibited the proliferation, migration and invasion of OC cells. Mechanistically, a dual-luciferase reported assay showed that RIPK4 is a target gene of miR-330-3p. Furthermore, rescue experiments revealed that miR-330-3p suppressed the proliferation, migration and invasion of OC cells by targeting RIPK4. In summary, our findings indicated that miR-330-3p suppressed the progression of OC by targeting RIPK4. Our results indicated that miR-330-3p/RIPK4 axis might act as a novel therapeutic target for OC treatment.
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http://dx.doi.org/10.1080/21655979.2021.1871817DOI Listing
December 2021

Sleep quality and health-related quality of life in children who survived for more than 5 years after liver transplantation.

J Dig Dis 2020 Nov 10;21(11):668-670. Epub 2020 Sep 10.

Department of Liver Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

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http://dx.doi.org/10.1111/1751-2980.12931DOI Listing
November 2020

Network Pharmacology and Experimental Evidence Identify the Mechanism of Astragaloside IV in Oxaliplatin Neurotoxicity.

Drug Des Devel Ther 2021 12;15:99-110. Epub 2021 Jan 12.

Department of Traditional Chinese Medicine, Changzheng Hospital, Naval Medical University, Shanghai 200003, People's Republic of China.

Background And Objective: Neurotoxicity is a common side effect of oxaliplatin; the effect of current drugs such as methylcobalamin and gabapentine is not obvious. Astragaloside IV (AS-IV) is an important active ingredient of , which can protect the nervous system and inhibit tumor growth to a certain extent. However, whether AS-IV can reduce oxaliplatin neurotoxicity and its molecular mechanism remain unclear.

Methods: The network pharmacology method was used to determine the collective targets of AS-IV and oxaliplatin neurotoxicity. The model of neurotoxicity was established by intraperitoneal injection of oxaliplatin in rats. Bodyweight, mechanical withdrawal threshold (MWT), cold allodynia, and nerve conduction velocity (NCV) were examined, pathological changes were observed by hematoxylin-eosin staining, number of Nissl bodies were assessed by Nissl staining, the key collective targets were measured by spectrophotometry and immunohistochemistry.

Results: Through network pharmacological analysis, 25 collective targets of AS-IV and oxaliplatin neurotoxicity were identified, mainly related to inflammation and oxidative stress. AS-IV could increase body weight, elevate MWT, and reduce cold allodynia of model rats, it also raised NCV. Neuropathology was improved and the number of Nissl bodies was increased by AS-IV administration. It reduced TNF-α, IL-6, and IL-1β in the spinal cord of model rats to inhibit inflammation; it also decreased MDA, raised SOD, CAT, and GSH-Px in the spinal cord of model rats to block oxidative stress.

Conclusion: AS-IV improves oxaliplatin neurotoxicity by regulating neuroinflammation and oxidative stress; the results can provide a new perspective for the potential treatment strategy of oxaliplatin neurotoxicity.
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http://dx.doi.org/10.2147/DDDT.S262818DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7811377PMC
January 2021

The prevalence, risk factors, and prognostic value of venous thromboembolism in ovarian cancer patients receiving chemotherapy: a systematic review and meta-analysis.

World J Surg Oncol 2021 Jan 13;19(1):12. Epub 2021 Jan 13.

Department of Pathology, Jiangxi Maternal and Child Health Hospital, Nanchang, 330006, Jiangxi, China.

Background: Venous thromboembolism (VTE) in ovarian cancer (OC) patients has been widely investigated, but our knowledge on the role of VTE in OC patients receiving chemotherapy is limited. The aim of our study was to investigate the prevalence, risk factors, and prognostic value of chemotherapy-associated VTE in OC.

Methods: Three databases (PubMed, Embase, and the Cochrane Library) were systematically searched from inception to October 14, 2020. The primary outcome was the prevalence of VTE in OC patients receiving chemotherapy. The risk factors and prognostic value of VTE were the secondary outcomes. The pooled prevalence of VTE was estimated using the generic inverse-variance method. The statistical heterogeneity was evaluated with Cochran's Q test and I statistic. Funnel plot, Begg's test, and Egger's test were used to assess the potential publication bias in the meta-analysis.

Results: A total of eleven observational studies with 4759 OC patients were included. The pooled prevalence of VTE was 9% (95% CI, 0.06-0.12) in OC patients receiving chemotherapy. The results of subgroup analysis and sensitivity analysis were basically consistent with the overall pooled estimate. Multiple significant risk factors associated with VTE were also identified including advanced age, D-dimer > 0.5 mg/mL, and tumor diameter > 10 cm. Only two included studies reported the prognostic value of VTE in OC patients receiving chemotherapy, but with inconsistent results. Funnel plot showed that there existed potential publication bias, which was further verified by statistical test, but the results of the trim-and-fill method showed the pooled estimate kept stable after adding two "missing" studies.

Conclusions: This current study revealed that the pooled prevalence of chemotherapy-related VTE in OC was approximately 9% in OC patients. Risk factors for chemotherapy-related VTE were also identified which may contribute to targeting potentially preventative measures for VTE in OC.
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http://dx.doi.org/10.1186/s12957-020-02101-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7807708PMC
January 2021

Fighting against COVID-19: preparedness and implications on clinical practice in primary care in Shenzhen, China.

BMC Fam Pract 2020 12 18;21(1):271. Epub 2020 Dec 18.

Department of Family Medicine and Primary Care, Li Ka Shing Faculty of Medicine, The University of Hong Kong, 3/F, Ap Lei Chau Clinic, 161 Main Street, Ap Lei Chau, Hong Kong.

Background: The new coronavirus pneumonia (NCP) caused by COVID-19 has affected more than 46 million people worldwide. In China, primary care has played a vital role during the COVID-19 outbreak, and it is important to examine the challenges faced by general practitioners (GPs). This study investigated the roles, preparedness and training needs of GPs in China in managing the NCP outbreak. Based on the outcomes of the study, we hope to take lessons and identify how GPs could be supported in delivering their gatekeeping roles and clinical duties in times of infectious disease outbreak.

Methods: An online survey on the official website of Shenzhen Continuing Education Center. It included questions on GPs' demographics, their awareness of COVID-19 and their preparedness in managing suspected cases of NCP, as well as referrals and their training needs. Conditional multi-variate logistic models were used to investigate the relationships between GPs' preparedness, situational confidence and anxiety.

Results: GPs' clinical practice was significantly affected. GPs endeavoured to answer a flood of COVID-19-related enquiries, while undertaking community preventive tasks. In addition to in-person consultations, GP promoted COVID-19 awareness and education through telephone consultations, physical posters and social media. Overall GPs in Shenzhen felt well supported with adequate Personal Protective Equipment (PPE) and resources from secondary care services. Higher levels of self-perceived preparedness (OR = 2.19; 95%CI, 1.04-4.61), lower level of anxiety (OR = 0.56; 95%CI, 0.29-1.09) and fewer perceived family worries (OR = 0.37; 95%CI, 0.12-1.12) were associated with better confidence in coping at work.

Conclusions: Training and supporting GPs while reducing their (and their families') anxiety increase their confidence in delivering the important roles of gatekeeping in face of major disease outbreaks.
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http://dx.doi.org/10.1186/s12875-020-01343-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7747774PMC
December 2020

Therapeutic Effects of 17β-Estradiol on Pelvic Organ Prolapse by Inhibiting Mfn2 Expression: An Study.

Front Endocrinol (Lausanne) 2020 25;11:586242. Epub 2020 Nov 25.

Department of Obstetrics and Gynecology, Peking University First Hospital, Beijing, China.

Objective: To assess the effects of 17β-estradiol (E2) on proliferation, apoptosis, and protein expressions of fibroblasts at different concentrations and time intervals to reveal the mechanism of E2 in the treatment of pelvic organ prolapse (POP).

Study Design: The uterosacral ligament fibroblasts were collected from seven POP patients for primary culture of fibroblasts. The culture media containing 0, 10, 10, 10, and 10 mol/L E2 were used for 24, 48, 72, and 96 h.

Main Outcome Measures: The cells were collected for cell counting kit-8 (CCK-8), apoptosis, quantitative reverse transcription polymerase chain reaction (qRT-PCR), and Western blotting assays.

Results: Compared with the control group, in the values of fibroblasts cultured in 10 mol/L E2 for 72 h, the proliferation, mRNA and protein expression of Mitofusin-2 (Mfn2) separately increased (P < 0.05), decreased (P<0.001) and decreased (P<0.001). However, the expression level of procollagen 1A1/1A2/3A1 and cyclinD1 markedly increased (P<0.001, all), which was consistent with the results of protein level. What's more, the expression of estrogen receptor α(ERα), estrogen receptor β(ERβ) and G protein-coupled receptor 30(GPR30) were significantly increased in 10 mol/L E2 group.

Conclusions: E2 can inhibit the progress of POP by inhibiting the expression level of Mfn2, as well as promoting expression of procollagens and proliferation of fibroblasts. This effect is time- and concentration-dependent. Only when the estrogen concentration reaches 10 mol/L, the therapeutic effect is the greatest after 72 h.
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http://dx.doi.org/10.3389/fendo.2020.586242DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7726108PMC
November 2020

Meta-Analysis Based on Clinical RCTs: The Effect of Molecular Epimerism on the Safety of Glycyrrhizic Acid.

Evid Based Complement Alternat Med 2020 30;2020:3869698. Epub 2020 Nov 30.

Department of Pharmacy, Suqian People's Hospital of Nanjing Drum Tower Hospital Group, Suqian 223800, Jiangsu, China.

Objective: To carry out the meta-analysis on the clinical safety of glycyrrhizic acid and the influencing factors between 18-glycyrrhizinate (18-GL) and 18-glycyrrhizinate (18-GL).

Methods: Magnesium isoglycyrrhizinate injection was used as the representative preparation of 18-GL, and compound glycyrrhizin injection was used as the representative preparation of 18-GL. The clinical control trial of magnesium isoglycyrrhizinate injection and compound glycyrrhizin injection was searched in a computer, which was published from January 2006 to December 2019 on the databases such as PubMed, China National Knowledge Infrastructure (CNKI), China Science and Technology Journal Database (CSTJ), and Wanfang Medical Network (Wanfang Data). The data associated with adverse drug reactions (ADRs) were extracted. RevMan5.3 was used for statistical analysis.

Results: Finally, 24 studies were included, and 2757 patients were involved, of which the experimental group was mainly treated with magnesium isoglycyrrhizinate, while the control group was mainly treated with compound glycyrrhizin. The results showed that the occurrence of ADRs was significantly lower in the experimental group than that in the control group, and the difference between two groups was statistically significant (RR = 0.26, 95% CI = (0.18, 0.38), < 0.00001). There was no heterogeneity among the studies ( = 0%, =1.00).

Conclusion: Compared with 18-GL, 18-GL had a lower incidence of adverse reactions and better clinical safety.
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http://dx.doi.org/10.1155/2020/3869698DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7721498PMC
November 2020

Antihypertensive drugs are associated with reduced fatal outcomes and improved clinical characteristics in elderly COVID-19 patients.

Cell Discov 2020 Oct 29;6(1):77. Epub 2020 Oct 29.

The State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Department of Biochemistry, Peking Union Medical College, Beijing, 100005, China.

The novel coronavirus (CoV) severe acute respiratory syndrome (SARS)-CoV-2 outbreak began at the end of 2019 in Wuhan, China, and has spread to over 200 countries. In this multicenter retrospective study, we identified 2190 adult patients admitted for laboratory-confirmed COVID-19 in three participating centers. Multivariate logistic regression was conducted in patients with comorbid hypertension to examine the potential association between clinical outcomes, disease severity, and clinical characteristics with the use of ACEI, ARB, calcium-channel blockers (CCB), beta-blockers (BB), and thiazide diuretics. The clinical outcome, dyspnea, and fatigue were significantly improved in patients, especially elderly patients who were older than 65 years, who took ARB drugs prior to hospitalization compared to patients who took no drugs. The reduction of disease severity of elderly COVID-19 patients was associated with CCB and ACEI users. Clinical indices, including CRP, lymphocyte count, procalcitonin D dimer, and hemoglobin, were significantly improved in elderly ARB users. In addition, the clinical outcomes were statistically significantly improved in patients who took antihypertension drugs ARB, BB, and CCB after statistical adjustment by all ages, gender, baseline of blood pressures, and coexisting medical conditions. Our data indicate that hypertension drugs ARB, ACEI, CCB, and BB might be beneficial for COVID-19 patients.
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http://dx.doi.org/10.1038/s41421-020-00221-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7595708PMC
October 2020

Distribution of serum specific IgE in children with allergic conjunctivitis and analysis of its concomitant allergic diseases.

Transl Pediatr 2020 Oct;9(5):636-644

Department of Optometry Center, Xi'an People's Hospital (Xi'an Fourth Hospital), Xi'an, China.

Background: Allergic conjunctivitis (AC) is predominantly caused by serum specific-IgE (sIgE)-mediated type I allergy. This study aims to analyze the distribution of sIgE in children with AC, and the concomitant allergic diseases.

Methods: The clinical data from 310 children, diagnosed with AC and admitted to our hospital from January 2017 to January 2019 were retrospectively collected. The children were divided into three groups: infant group (2 months to 1 year old, 91 cases), child group (1 to 3 years old, 112 cases), and preschool group (3 to 6 years old, 107 cases). Children in every group were analyzed for positive rates, the number of positive sIgE types, the distribution of positive inhaling and ingesting allergens and concomitant allergic diseases.

Results: The sIgE positive rate of infant was significantly lower than that of the other two groups, and the number of 18.75% sIgE positive species was 1. The number of sIgE positive species in the child group and preschool group was more than 2 (78.30%, 71.15%). The positive rate of sIgE to dust mites, house dust, animal dander, eggs, beef, mutton and mango in the preschool group was significantly higher than the other groups (P<0.05). The positive rate of sIgE to milk in infant group was significantly higher than the other two groups (P<0.05). Children in the preschool group showed the highest incidence of AC, AC + allergic rhinitis, AC + allergic rhinitis + wheezing, while those in the infant group displayed the lowest incidence (P<0.05). AC + gastrointestinal allergy, AC + atopic dermatitis, AC + gastrointestinal allergy + atopic dermatitis in infant group was significantly higher than the other two groups (P<0.05). Patients in the child group displayed a significantly higher incidence of AC + infant wheezing than the other two groups (P<0.05).

Conclusions: We correlated children's age with the positive rate and gradual increase in types of AC allergens. Concomitant allergic diseases of children with AC at different ages conform to the natural course of allergic diseases. In clinic, improving the diagnostic efficiency of AC in children, and early interventional treatment will positively contribute to their prognosis, and reduce the risk of other allergic diseases.
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http://dx.doi.org/10.21037/tp-20-216DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7658764PMC
October 2020

Concurrent identification of 11 major primary active compounds in Huangqin Qingfei Decoction by liquid chromatography tandem mass spectrometry via liquid chromatography tandem mass spectrometry.

Pak J Pharm Sci 2020 May;33(3):1005-1013

School of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Huangqin Qinfei Decoction (HQD) is a traditional Chinese medicine that is administered for acute pneumonia, bronchial inflammation, acute bronchitis and acute lung infection. In this study, we used liquid chromatography linked with tandem mass spectrometry (LC-MS/MS) for the concurrent identification of 11 bioactive compounds; namely, baicalin, baicalein, wogonoside, scutellarin, wogonin, oroxylin A, geniposide, genipin, geniposidic acid, chlorogenic acid, and crocin-I, for the quality control of HQD. The evaluation was conducted on an Agilent Poroshell 120 EC-C18 (2.1mm×100mm, 2.7μm) with gradient elution in the mobile phase with 0.1% formic acid and 1mM/L ammonium acetate in water as solvent A and methanol as solvent B at a flow rate of 0.3mL/min in under 12 min. Mass spectrometric detection was conducted in the selected reaction monitoring mode utilizing electro spray ionization in the positive and negative modes. Every one of the calibration curves had good linearity with R2 >0.9992. Intra-day and inter-day accuracies for every one of the evaluated components were expressed as the relative standard deviation (RSD) from 1.72%-5.02% and 0.63%-5.99%, respectively. The recuperation of the 11 compounds that were measured at the three concentrations was within 94.05%-105.18%, with the RSD ≤ 6.26%. The use of this method was determined through the effective evaluation of 11 compounds in 5 batches of HQD. The confirmed method is precise, sensitive, and effective for identifying the contents of the chosen compounds in HQD for quality control.
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May 2020

The influence of the COVID-19 epidemic on the gastrointestinal endoscopy practice in China: a national survey.

Surg Endosc 2020 Nov 11. Epub 2020 Nov 11.

Department of Gastroenterology, The First Medical Center of Chinese PLA General Hospital, No. 28, Fuxing Road, Haidian District, Beijing, 100853, China.

Background And Aim: With the worldwide spread of coronavirus disease 2019 (COVID-19), it has devastated the economy and taken a toll on people' life in every aspects. In this study, we aimed to evaluate the influence of the COVID-19 epidemic on the GI endoscopy practice in China.

Methods: The nationwide survey conducted from 13 to 19 April, 2020. A predesigned standard structured questionnaire was sent to all members of the Chinese Society of Digestive Endoscopy (CSDE) in mainland China by email. Number of various GI endoscopic procedures and participants getting endoscopic training from January 1 to April 10, 2020 (the COVID-19 period) and the same period of 2019 were collected and analyzed.

Results: A total of 468 hospitals responded to this survey, and most of them (85.4%) were tertiary referral hospitals. The sum number of GI endoscopic procedures deceased significantly from 3,203,594 in 2019 to 1,512,619 in 2020, including 2,996,779 to 1,401,665 of diagnostic procedures and 206,815 to 110,954 of therapeutic procedures. More than half of centers (57.1%) reduced about 1,000-5,000 endoscopic activities. Of 271 hospitals (57.9%) providing endoscopic training, the total number of participants decreased from 2,977 in 2019 to 1,131 in 2020. Most of hospitals (93.8%) adhered to the recommendation of endoscopy practice issued by CSDE during the outbreak of COVID-19, and there was no cases of infection in endoscopic departments of all surveyed hospitals.

Conclusion: With the influence of the COVID-19 epidemic, there has been significant decease of GI endoscopy practice in mainland China.
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http://dx.doi.org/10.1007/s00464-020-08149-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7657378PMC
November 2020

Downregulation of PHLPP1 ameliorates high glucose-evoked injury in retinal ganglion cells by attenuating apoptosis and oxidative stress through enhancement of Nrf2 activation.

Exp Cell Res 2020 12 24;397(2):112344. Epub 2020 Oct 24.

Ophthalmology Department, The Second Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an, 710004, PR China. Electronic address:

High glucose (HG)-induced oxidative stress contributes significantly to the pathogenesis of diabetic retinopathy. Pleckstrin homology domain and leucine rich repeat protein phosphatase 1 (PHLPP1) has emerged as a key regulator of oxidative stress implicated in various pathological processes. However, whether PHLPP1 participates in the regulation of HG-induced oxidative stress injury of retinal ganglion cells (RGCs) in diabetic retinopathy is undetermined. The purpose of this study was to explore the potential role and molecular mechanism of PHLPP1 in regulating HG-induced injury of RGCs. Our data showed that PHLPP1 expression was markedly elevated in RGCs from diabetic rats and HG-exposed RGCs. Our functional assay elucidated that knockdown of PHLPP1 improved cell viability and decreased cell apoptosis and reactive oxygen species (ROS) production in HG-exposed RGCs. Additionally, upregulation of PHLPP1 lowered cell viability and increased cell apoptosis and ROS production in HG-exposed RGCs. Mechanistically, knockdown of PHLPP1 resulted in an increase in nuclear factor erythroid-2 related factor 2 (Nrf2) nuclear expression and Nrf2/antioxidant response element (ARE)-mediated transcription associated with upregulation of glycogen synthase kinase-3β (GSK-3β) phosphorylation. Moreover, inhibition of GSK-3β significantly reversed the suppressive effect of PHLPP1 overexpression on Nrf2/ARE activation. Notably, the protective effect of PHLPP1 knockdown on HG-induced injury in RGCs was markedly abolished by Nrf2 inhibition. In conclusion, Our findings demonstrate that downregulation of PHLPP1 activates Nrf2/ARE signaling to protect RGCs from HG-induced apoptosis and oxidative stress. This study indicates a potential role of PHLPP1 in regulating HG-induced injury of RGCs during the development and progression of diabetic retinopathy.
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http://dx.doi.org/10.1016/j.yexcr.2020.112344DOI Listing
December 2020

Sustainable control of the rice pest, Nilaparvata lugens, using the entomopathogenic fungus Isaria javanica.

Pest Manag Sci 2021 Mar 28;77(3):1452-1464. Epub 2020 Nov 28.

Key Laboratory of Bio-Pesticide Creation and Application of Guangdong Province, Department of Entomology, College of Plant Protection, South China Agricultural University, Guangzhou, China.

Background: The brown planthopper (Nilaparvata lugens) is an insect pest of rice (Oryza sativa) that is distributed worldwide and is responsible for significant crop yield losses. Of particular concern, N. lugens has developed high resistance to several commonly used insecticides. The entomopathogenic fungus, Isaria javanica, offers an alternative to insecticides for the control of rice plant pests.

Results: We show that I. javanica caused high mortality of N. lugens nymphs and reduced the survival and fecundity of newly emerged adults in I. javanica-treated insects. I. javanica persisted on plants up to 17 days after its inoculation under greenhouse conditions carried out in summer and autumn. Rice metabolites supported conidial germination and mycelia growth of I. javanica. A granular formulation of I. javanica conidia controlled N. lugens populations in a field experiment. The granular formulation promoted fungal survival and growth in the field up to 28 days post inoculation, resulting in increased fungal persistence and greater pest control coverage.

Conclusion: These data indicate that a granular formulation of I. javanica is able to control N. lugens populations in rice fields. Therefore, this granular formulation can be an alternative to insecticides and can be used in organic fields. © 2020 Society of Chemical Industry.
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http://dx.doi.org/10.1002/ps.6164DOI Listing
March 2021

Comparison of the Efficacy of Two Laparoscopic Surgical Procedures Combined with Hysteroscopic Incision in the Treatment of Cesarean Scar Diverticulum.

J Invest Surg 2020 Oct 15:1-6. Epub 2020 Oct 15.

Department of Obstetrics and Gynecology, Peking University First Hospital, Beijing, China.

Objective: To investigate the efficacy of two different surgical procedures in combined hysteroscopic and laparoscopic surgery-the "folding suture method" and the "muscle flap filling suture method"-in the treatment of cesarean scar diverticulum (CSD).

Methods: The clinical data of 24 patients with CSD who underwent surgery in the Peking University First Hospital from August 2016 to December 2018 were retrospectively analyzed.

Results: There was no difference in age, vaginal bleeding time, thickness of the lower uterine segment, operative time and intraoperative bleeding between the two groups. At three months after the operation, the patients of the folding suture and muscle flap groups had an average menstrual period of 6.9 ± 1.8 days and 7.5 ± 3.0 days, respectively, which was 5.8 ± 4.2 days and 4.4 ± 3.8 days, respectively, shorter than that before the operation, as well as a lower segment thickness of the uterus of 6.7 ± 1.8 mm and 6.3 ± 1.7 mm, respectively. Among the patients in the folding suture and muscle flap groups, 8 and 6 cases were cured, and 3 and 6 cases were improved, respectively, resulting in an effective rate of 100%. There was no significant difference in any indicator between the two groups.

Conclusion: As two new surgical methods that preserve uterine integrity, the laparoscopic "muscle flap filling suture method" and "folding suture method" combined with hysteroscopic incision are safe and effective treatments for repairing CSD.
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http://dx.doi.org/10.1080/08941939.2020.1830319DOI Listing
October 2020

Heterogeneous photocatalytic anaerobic oxidation of alcohols to ketones by Pt-mediated hole oxidation.

Chem Commun (Camb) 2020 Oct 4;56(79):11847-11850. Epub 2020 Sep 4.

Inner Mongolia Key Laboratory of Carbon Nanomaterials, Nano Innovation Institute (NII), College of Chemistry and Materials Science, Inner Mongolia University for Nationalities Tongliao, 028000, China.

We report a platinum nanocluster/graphitic carbon nitride (Pt/g-CN) composite solid catalyst with a photocatalytic anaerobic oxidation function for highly active and selective transformation of alcohols to ketones. The desirable products were successfully obtained in good to excellent yields from various functionalized alcohols at room temperature, including unactivated alcohols. Mechanistic studies indicated that the reaction could proceed through a Pt-mediated hole oxidation initiating an α-alcohol radical intermediate followed by a two-electron oxidation pathway. The merit of this strategy offers a general approach towards green and sustainable organic synthetic chemistry.
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http://dx.doi.org/10.1039/d0cc03325aDOI Listing
October 2020

: A Calcineurin B-Like Protein-Interacting Protein Kinase From the Halophyte , Enhances Arabidopsis Salt Tolerance.

Front Plant Sci 2020 21;11:1112. Epub 2020 Aug 21.

Key Laboratory of Forestry Genetics & Biotechnology of Ministry of Education, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China.

Calcineurin B-like protein-interacting protein kinases () play essential roles in plant abiotic stress response. In order to better understand salt tolerance, we cloned and analyzed the gene from the halophyte . Phylogenetic analysis shows that belongs to a sister clade with the gene and is thought to localize to the plasma membrane. shows the highest expression level in the root under normal growth conditions, whereas after NaCl treatment, the highest expression was found in the blade. -overexpressing Arabidopsis plants have a higher seed germination rate, longer root length, and displayed higher salt tolerance than wild type seedlings under salt stress conditions. Furthermore, overexpression might enhance the expression of genes related to K transportation after NaCl treatment. Thus, we conclude that increases transgenic plant salt tolerance and reduces damage associated with salt stress by promoting the expression of genes controlling ion homeostasis. Our results suggest that could serve as an ideal candidate gene to genetically engineer salt-tolerant plants.
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http://dx.doi.org/10.3389/fpls.2020.01112DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7472804PMC
August 2020

Deletion of Bmal1 Impairs Pancreatic -Cell Function via Mitochondrial Signaling Pathway.

Biomed Res Int 2020 7;2020:9803024. Epub 2020 Sep 7.

Department of Rheumatology, School of Medicine, The Second Affiliated Hospital of Zhejiang University, No. 88, Jiefang Road, Hangzhou 310009, China.

Several studies have demonstrated that brain and muscle Arnt-like protein-1 (Bmal1) acts as a core clock gene for maintaining normal cell function, including hepatocytes and cardiomyocytes. Loss of Bmal1 is associated with type 2 diabetes due to pancreatic -cell failure. However, little information is available about its role and mechanism in pancreatic -cell. To address this, we investigated the consequences of Bmal1 inhibition in an insulinoma cell line (INS-1) by using small interfering RNA (siRNA). We observed that knockout of Bmal1 impaired glucose-stimulated insulin secretion in -cell. Meanwhile, the depletion of Bmal1 in -cell caused an adverse change in mitochondrial membrane potential and mitochondrial architecture. Deletion of Bmal1 attenuated mRNA and protein expression of mitofusin 1 (Mfn1) and mitofusin 2 (Mfn2) and enhanced the expression of fission 1 (Fis1). In summary, the deletion of Bmal1 impaired -cell function may be via the mitochondrial signaling pathway in INS-1 cells.
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http://dx.doi.org/10.1155/2020/9803024DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7492957PMC
September 2020

Comparative efficacy and safety of anti-vascular endothelial growth factor regimens for neovascular age-related macular degeneration: systematic review and Bayesian network meta-analysis.

Ther Adv Chronic Dis 2020 4;11:2040622320953349. Epub 2020 Sep 4.

Ophthalmology Department, The Second Affiliated Hospital, Medical College, Xi'an Jiaotong University, No. 157 Xiwu Road, Xi'an, 710004, PR China.

Background: As a debilitating neurodegenerative disease, neovascular age-related macular degeneration (nAMD) accounts for more than 90% of severe visual loss or legal blindness among AMD patients. Anti-vascular endothelial growth factor (VEGF) had been applied widely in nAMD treatment. To date, debate regarding efficacy and safety still exists among different anti-VEGF regimens as management of nAMD. To provide substantial evidence for clinical nAMD treatment, this study ranks the priority of anti-VEGF regimens Bayesian network meta-analysis (NMA), comparing data collected from randomized controlled trials (RCTs).

Methods: We searched PubMed Central, MEDLINE Ovid, Embase Ovid, ISRCTN, ICTRP and ClinicalTrials. gov from a database established until 1 April 2019 systematically for anti-VEGF regimens. Bayesian NMA with random-effect was conducted to compare efficacy and safety and rank priority of anti-VEGF regimens. The primary efficacy and safety outcomes were the proportion of patients gaining 15 or more letters, and the incidence of arterial thromboembolic (ATC) events. The effect measure is the standard mean difference (SMD), or the odds ratio (OR) with their 95% confidence interval (CI). The study protocol is registered with PROSPERO, number CRD42019132243.

Results: We obtained 6467 citations and identified 29 RCTs including 13,596 participants; 86% of these trials were low risk or of uncertain risk bias. In NMA, ORs compared with sham injection for the proportion of patients gaining 15 or more letters (12,699 participants from 23 trials) ranged from 4.05 [95% Bayesian credible interval (CrI) 1.62-10.11] for ranibizumab quarterly regimen to 8.57 (95% CrI 4.66-15.73) for a ranibizumab treat-and-extend regimen. No difference was found between sham injection and anti-VEGF regimens for ATC events (11,500 participants from 18 trials). Results for the primary outcome did not substantially change in sensitivity analyses after removing studies at high risk of bias and small sample size ( < 100), respectively.

Conclusion: The treat-and-extend regimen of ranibizumab and aflibercept are the preferred anti-VEGF regimens for nAMD. Bevacizumab treat-and-extend regimens need more head-to-head comparisons with other regimens or sham injection for advanced application. The treat-and-extend regimen proved to be the most effective regimen for each anti-VEGF drug in the NMA. Pegaptanib every 6 weeks and Conbercept quarterly are unable to satisfy the best corrected visual acuity (BCVA) improvement requirement of nAMD patients.
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http://dx.doi.org/10.1177/2040622320953349DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7475790PMC
September 2020

Risk prediction of in-hospital mortality among patients with type 2 diabetes mellitus and concomitant community-acquired pneumonia.

Ann Palliat Med 2020 Sep 8;9(5):3313-3325. Epub 2020 Sep 8.

Department of Pulmonary and Critical Care Medicine, Shengjing Hospital of China Medical University, Shenyang, China.

Background: The aim of the present study was to investigate the risk factors for in-hospital mortality among patients with type 2 diabetes mellitus (T2DM) and concomitant community-acquired pneumonia (CAP) and establish a risk prediction score.

Methods: Data from 1,360 adult patients with T2DM and concomitant CAP hospitalized in two grade 3A hospitals between 2009 and 2019 were collected through electronic medical records. Data obtained included the status of diabetes mellitus, comorbidities, laboratory and imaging findings, and treatment outcomes. Statistical analysis was conducted to investigate the risk factors affecting prognosis, and a clinical risk prediction score was designed.

Results: Based on the patients' treatment outcomes (deceased, improved and cured), the cohort was divided into two groups: deceased and improved; 16 parameters were significant after segmentation. However, the following nine parameters were independent predictors of mortality: neutrophil-lymphocyte ratio (NLR) ≥4, pulse rate ≥125 bpm, change in state of consciousness, arterial blood pH ≤7.35, age ≥65 years, serum sodium ≤130 mmol/L, initial fasting blood glucose ≥9 mmol/L, multilobar involvement, and diabetic nephropathy. Based on these findings, a risk prediction score was established, and bootstrap validation was performed. The risk prediction score was significantly superior to CURB-65 [confusion, urea >7 mmol/L, respiratory rate >30/min, low blood pressure (systolic <90 mmHg or diastolic <60 mmHg), age >65 years] and slightly superior than the pneumonia severity index (PSI).

Conclusions: The influencing factors for in-hospital mortality among patients with T2DM and concomitant CAP included advanced age, change in state of consciousness, increased pulse rate, acidosis, high NLR, high platelet-lymphocyte ratio, hyponatremia, hyperglycemia, and diabetic nephropathy. These parameters should be recognized in clinical practice, with active interventions to improve the treatment success rate. The risk prediction score effectively differentiated the mortality risk of inpatients, thereby providing guidance on clinical decision-making.
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http://dx.doi.org/10.21037/apm-20-1489DOI Listing
September 2020

Hidden Blade Scalpel: A Useful Tool for the Treatment of Axillary Osmidrosis.

Plast Surg (Oakv) 2020 Aug 8;28(3):156-160. Epub 2020 Apr 8.

Beijing Shijitan Hospital, Capital Medical University, Beijing, China.

Background: Axillary osmidrosis is a distressing problem that can particularly affect a patient's social life. In severe cases, patients may seek a surgical treatment to achieve a permanent effect. Many treatment techniques involving destruction or removal of the apocrine and eccrine glands have been developed. However, previous treatments have been hindered by surgical or aesthetic concerns. The purpose of this study was to evaluate the effect of the hidden blade scalpel procedure in treating axillary osmidrosis.

Methods: From December 2012 to December 2016, 372 patients with axillary osmidrosis underwent the hidden blade scalpel procedure. One 5-mm incision was made at approximately 1 cm beyond the axillary hairline. A hidden blade scalpel was then used to remove the subcutaneous tissue, including the plexus, apocrine and eccrine glands, and hair follicles, from the skin. The clinical efficacy was evaluated using a questionnaire.

Results: The hidden blade scalpel procedure resulted in a high percentage of patient satisfaction, a much shorter recovery time, and a low complication rate.

Conclusion: The hidden blade scalpel procedure is an effective method for the treatment of axillary osmidrosis.
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http://dx.doi.org/10.1177/2292550320914080DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7436846PMC
August 2020

Evaluation of disinfection procedures in a designated hospital for COVID-19.

Am J Infect Control 2021 04 22;49(4):447-451. Epub 2020 Aug 22.

Infection Control Department, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China. Electronic address:

Background: Coronavirus disease 2019 has spread globally and been a public health emergency worldwide. It is important to reduce the risk of healthcare associated infections among the healthcare workers and patients. This study aimed to investigate the contamination of environment in isolation wards and sewage, and assess the quality of routine disinfection procedures in our hospital.

Methods: Routine disinfection procedures were performed 3-times a day in general isolation wards and 6-times a day in isolated ICU wards in our hospital. Environmental surface samples and sewage samples were collected for viral RNA detection. Severe acute respiratory syndrome coronavirus 2 RNA detection was performed with quantitative reverse transcription polymerase chain reaction.

Results: A total of 163 samples were collected from February 6 to April 4. Among 122 surface samples, 2 were positive for severe acute respiratory syndrome coronavirus 2 RNA detection. One was collected from the flush button of the toilet bowl, and the other was collected from a hand-basin. Although 10 of the sewage samples were positive for viral RNA detection, all positive samples were negative for viral culture.

Conclusion: These results revealed the routine disinfection procedures in our hospital were effective in reducing the potential risk of healthcare associated infection. Two surface samples were positive for viral detection, suggesting that more attention should be paid when disinfecting places easy to be ignored.
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http://dx.doi.org/10.1016/j.ajic.2020.08.028DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7443056PMC
April 2021

Stereoselective Synthesis of a Topologically Chiral Solomon Link.

J Am Chem Soc 2020 08 31;142(32):13667-13671. Epub 2020 Jul 31.

State Key Laboratory of Molecular Engineering of Polymers, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Department of Chemistry, Fudan University, Shanghai 200433, People's Republic of China.

With the help of retrosynthetic analysis, we have realized the highly anticipated stereoselective synthesis of a topologically chiral Solomon link, by taking advantage of coordination-driven self-assembly and chiral induction by axially chiral ligands. Combination of the ligands ( or )-2,2'-diethoxy-1,1'-binaphthyl-6,6'-bis(4-vinylpyridine) ( or ) with the binuclear iridium complex [Cp*Ir(DHBQ)(OTf)] ((OTf), HDHBQ = 2,5-dihydroxy-1,4-benzoquinone) allows diastereoselective synthesis of the topological enantiomers () or () of a Solomon link, respectively. The main driving force for the formation of the Solomon link is the π-π interactions between chiral ligands.
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http://dx.doi.org/10.1021/jacs.0c05366DOI Listing
August 2020