Publications by authors named "Yaxian Li"

7 Publications

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Identification of Therapeutic Targets and Prognostic Biomarkers Among CXC Chemokines in the Renal Cell Carcinoma Microenvironment.

Front Oncol 2019 5;9:1555. Epub 2020 Feb 5.

Department of Hepatology, Gaozhou People's Hospital, Maoming, China.

Renal cell carcinoma (RCC) is one of the most common malignances with an ever-increasing incidence and high mortality. Cross-talk between cancer cells and interstitial cells exerts significant effects on neoplasia and tumor development and is modulated in part by chemokines. CXC chemokines in the tumor microenvironment can modulate immune cell trafficking and regulate tumor cell activities, thus exerting anti-tumor immunological effects and affecting patient outcomes; however, the expression and prognostic values of CXC chemokines in RCC have not been clarified. ONCOMINE, GEPIA, UALCAN, cBioPortal, GeneMANIA, DAVID 6.8, Metascape, TRRUST, LinkedOmics, and TIMER were utilized in this study. The transcriptional levels of in RCC tissues were significantly elevated while the transcriptional levels of CXCL3/7/12/13 were significantly reduced. A significant correlation was found between the expression of and the pathological stage of RCC patients. RCC patients with low transcriptional levels of were associated with a significantly better prognosis. The functions of differentially expressed CXC chemokines are primarily related to the chemokine signaling pathway, cytokine-cytokine receptor interactions, and the ILK signaling pathway. Our data suggest that RELA, NFKB1, and SP1 are key transcription factors for CXC chemokines, and the SRC family of tyrosine kinases (LCK, LYN, and FYN), mitogen-activated protein kinases (MAPK1 and MAPK3), and CSNK1D are CXC chemokine targets. We found significant correlations among the expression of CXC chemokines and the infiltration of six types of immune cells (B cells, CD8 T cells, CD4 T cells, macrophages, neutrophils, and dendritic cells). Our results may provide novel insights for the selection of immunotherapeutic targets and prognostic biomarkers for renal cell carcinoma.
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http://dx.doi.org/10.3389/fonc.2019.01555DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7012904PMC
February 2020

Construction of a Competitive Endogenous RNA Network in Uterine Corpus Endometrial Carcinoma.

Med Sci Monit 2019 Oct 25;25:7998-8010. Epub 2019 Oct 25.

Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China (mainland).

BACKGROUND Long non-coding RNAs (lncRNAs) affect post-transcriptional regulation by interfering with microRNAs (miRNAs), and by acting as competitive endogenous RNAs (ceRNAs). The roles and mechanisms of lncRNAs as ceRNAs in the progression and prognosis of uterine corpus endometrial carcinoma are not well understood. MATERIAL AND METHODS We analyzed high-throughput transcriptome data downloaded from The Cancer Genome Atlas database for 548 patients with uterine corpus endometrial carcinoma, and the we constructed a ceRNA network. Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analyses of differentially expressed messenger RNAs (DE-mRNAs) were performed using R software. Kaplan-Meier survival curves were generated for all RNAs in the ceRNA network. RESULTS We identified 2612 messenger RNAs (mRNAs), 1111 lncRNAs, and 187 miRNAs that were differentially expressed in uterine corpus endometrial carcinoma. We then identified mutual regulatory relationships between lncRNA-miRNA pairs and miRNA-mRNA pairs. A ceRNA regulatory network for uterine corpus endometrial carcinoma was successfully constructed, and consisted of 87 lncRNAs, 74 mRNAs, and 20 miRNAs. Nine lncRNAs, 3 miRNAs, and 22 mRNAs were associated with prognosis of uterine corpus endometrial carcinoma. We also analyzed the linear relationships between the expression of the 9 DE-lncRNAs and 22 DE-mRNAs with prognostic value. CONCLUSIONS Our study showed that the lncRNAs C2orf48 and LINC00261 might be key regulators of uterine corpus endometrial carcinoma and might serve as prognostic indicators. Our study contributes to the understanding of the molecular mechanisms of uterine corpus endometrial carcinoma, and it identifies lncRNAs that might serve as prognostic markers and therapeutic targets.
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http://dx.doi.org/10.12659/MSM.915798DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6825398PMC
October 2019

Adsorptive Removal of Acetaldehyde from Propylene Oxide Produced by the Hydrogen Peroxide to Propylene Oxide Process.

ACS Omega 2018 Nov 12;3(11):15272-15280. Epub 2018 Nov 12.

State Key Laboratory of Heavy Oil Processing, College of Chemical Engineering, China University of Petroleum, No. 66 West Changjiang Road, Qingdao 266580, P. R. China.

Adsorption method was first introduced into the propene oxide production via hydrogen peroxide process to remove the microimpurity in the propylene oxide (PO) product solution. It could replace the reactive distillation in separating acetaldehyde with less energy consumption and PO loss. A series of adsorbents (e.g., 3A, 4A, 5A, 10X, and Y) are first used to remove the impurity (i.e., acetaldehyde). It is found that 5A molecular sieves shows the best performance due to uniform porous channels with suitable pore size. Various techniques such as X-ray diffraction, scanning electron microscopy, thermogravimetric analysis, Fourier transform infrared, and N physisorption are employed to investigate the structural properties of the adsorbent. Furthermore, effects of space velocity and temperature are also investigated. Cyclic desorption and adsorption tests indicate the PO yield is 92.2%, and 96.3% of acetaldehyde was removed. The acetaldehyde concentration of PO product was 0.0187%, indicating this method can produce industrial-quality PO that meets the first-level technical requirements.
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http://dx.doi.org/10.1021/acsomega.8b02297DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6644169PMC
November 2018

Effect of β-endoxylanase and α-arabinofuranosidase enzymatic hydrolysis on nutritional and technological properties of wheat brans.

Food Chem 2020 Jan 5;302:125332. Epub 2019 Aug 5.

Department of Food Science and Nutrition, College of Food and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210023, PR China; Huaian Institute of Nanjing Normal University, Huaian 223200, PR China.

Wheat bran (WB) was treated using xylanase and arabinofuranosidase from Thermotoga maritima and added to steamed breads on 15% flour weight basis. The antioxidant capacity and water and oil retention capacity of brans were increased while their soluble xylooligosaccharides and phenolic acids content were increased. Two enzymes treatment was found to be more effective in decreasing the resistance to extension, softening degree, water absorption and development time, and in increasing the extensibility, stability time, porosity and sensorial characteristics of the steamed breads. Two enzymes treatment had significantly (P < 0.05) greater specific volume, springiness and cohesiveness and lower crumb firmness, gumminess, chewiness than single enzyme treatment. All results highlighted that combination of xylanase and arabinofuranosidase can improve the degrees hydrolysis of WB and its soluble AX xylooligosaccharides produced, having a synergetic effect on the dough rheology and nutritional and quality characteristics of steamed bread.
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http://dx.doi.org/10.1016/j.foodchem.2019.125332DOI Listing
January 2020

A 7-lncRNA signature predict prognosis of Uterine corpus endometrial carcinoma.

J Cell Biochem 2019 10 6;120(10):18465-18477. Epub 2019 Jun 6.

Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Current research indicate that long noncoding RNAs (lncRNAs) are associated with the progression of various cancers and can be used as prognostic biomarkers. This study aims to construct a prognostic lncRNA signature for the risk assessment of Uterine corpus endometrial carcinoma (UCEC). The RNA-Seq expression profile and corresponding clinical data of UCEC patients obtained from The Cancer Genome Atlas database. First, some prognosis-related lncRNAs were obtained by univariate Cox analysis. The minimum absolute contraction and selection operator (LASSO) regression and the Cox proportional hazard regression method were used to further identify the lncRNA prognostic model. Finally, seven lncRNAs (AC110491.1, AL451137.1, AC005381.1, AC103563.2, AC007422.2, AC108025.2, and MIR7-3HG) were identified as potential prognostic factors. According to the model constructed by the above analysis, the risk score of each UCEC patient was calculated, and the patients were classified into high and low-risk groups. The low-risk group had significant survival benefits. Moreover, we constructed a nomogram that incorporated independent prognostic factors (age, tumor stage, tumor grade, and risk score). The c-index value for evaluating the predictive nomogram model was 0.801. The area under the curve was 0.797 (3-year survival). The calibration curve also showed that there was a satisfactory agreement between the predicted and observed values in the probability of 1-, 3-, and 5-year overall survival. On the basis of the coexpression relationship, we established a coexpression network of lncRNA-messenger RNA (mRNA) of the 7-lncRNA. The Kyoto Encyclopedia of Genes and Genomes analysis of the coexpressing mRNAs showed that the main pathways related to the 7-lncRNA signature were neuroactive ligand-receptor interaction, serotonergic synapse, and gastric cancer pathway. Therefore, our study revealed that the 7-lncRNA could be used to predict the prognosis of UCEC and for postoperative treatment and follow-up.
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http://dx.doi.org/10.1002/jcb.29164DOI Listing
October 2019

Characterization of a uronate dehydrogenase from Thermobispora bispora for production of glucaric acid from hemicellulose substrate.

World J Microbiol Biotechnol 2018 Jun 23;34(7):102. Epub 2018 Jun 23.

Department of Food Science and Nutrition, GinLing College, Nanjing Normal University, Nanjing, 210097, People's Republic of China.

A thermostable uronate dehydrogenase Tb-UDH from Thermobispora bispora was over-expressed in Escherichia coli using the T7 polymerase expression system. The Tb-UDH was purified by metal affinity chromatography, and gave a single band on SDS-PAGE. The maximum activity on glucuronic acid was found at 60 °C and pH 7.0. The purified enzyme retained over 58% of its activity after holding a pH ranging from 7.0 to 7.5 for 1 h at 60 °C. The K and V values of the purified Tb-UDH for Glucuronic acid (GluUA) were 0.165 mM and 117.7 U mg, respectively, those for galacturonic acid (GalUA) were 0.115 mM and 104.2 U mg, respectively, and those for NAD were 0.120 mM and 133.3 U mg, respectively; the turnover number (k) with GluUA as a substrate was higher than that with GalUA; however, the Michaelis constant (K) for GalUA was lower than that for GluUA. After 60 min of incubation at 50 °C, Tb-UDH exhibited a conversion ratio for glucuronic acid to the glucaric acid of 84% on chemical reagent and 81.3% on hydrolysates from breech xylans formed by xylanase and α-glucuronidase. This work shows that biocatalytic routes have great potential for the conversion of hemicellulose substrate into value-added products derived from renewable biomass. TOC GRAPHIC: (A) The structure of the xylan is described and the site of action of the xylan degrading enzyme is indicated. (B) The effect of substrate concentration on recombinant Tb-UDH activity when galacturonic acid was used as substrate. (C) SDS-PAGE analysis of E. coli BL21 (DE3) harboring pET-20b(+) and pET-20b-Tb-UDH. (D) Oxidative conversion of glucuronic acid from a beechwood xylan to glucaric acid.
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http://dx.doi.org/10.1007/s11274-018-2486-8DOI Listing
June 2018

Cloning, expression and identification of KTX-Sp4, a selective Kv1.3 peptidic blocker from .

Cell Biosci 2017 6;7:60. Epub 2017 Nov 6.

School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan, 430074 People's Republic of China.

Background: Specific and selective peptidic blockers of Kv1.3 channels can serve as a valuable drug lead for treating T cell-mediated autoimmune diseases, and scorpion venom is an important source of kv1.3 channel inhibitors. Through conducting transcriptomic sequencing for the venom gland of from Xizang province of China, this research aims to discover a novel functional gene encoding peptidic blocker of Kv1.3, and identify its function.

Results: We screened out a new peptide toxin KTX-Sp4 which had 43 amino acids including six cysteine residues. Electrophysiological experiments indicated that recombinant expression products of KTX-Sp4 blocked both endogenous and exogenous Kv1.3 channel concentration-dependently, and exhibited good selectivity on Kv1.3 over Kv1.1, Kv1.2, respectively. Mutation experiments showed that the Kv1 turret region was responsible for the selectivity of KTX-Sp4 peptide on Kv1.3 over Kv1.1.

Conclusions: This work not only provided a novel lead compound for the development of anti autoimmune disease drugs, but also enriched the molecular basis for the interaction between scorpion toxins and potassium channels, serving as an important theoretical basis for designing high selective Kv1.3 peptide inhibitors.
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http://dx.doi.org/10.1186/s13578-017-0187-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5674823PMC
November 2017
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